CN109170060A - A kind of beverage and preparation method thereof for improving mood - Google Patents

Abstract

The present invention provide it is a kind of for improving the beverage of mood, by as follows at being grouped as: safflower 0.01-0.02 parts by weight, rose 0.5-1.5 parts by weight, bitter orange flower 0.5-1.5 parts by weight, arillus longan 0.5-1.5 parts by weight.The present invention also provides the preparation methods of above-mentioned beverage, comprising: 1) mixes safflower with two taste medicinal material of rose, beat powder, mixture I is made in 20~80 mesh of partial size, spare；2) arillus longan is dried under vacuum to moisture < 8%, the mixing of equivalent bitter orange flower is added and beats powder to 20~80 mesh of particle size range, mixture II is made；3) mixture I is uniformly mixed with mixture II, is made and brews mixture, can also be further processed into capsule or oral solution.The beverage has resolving stagnation for tranquilization, and improvement is depressed, improves the effect of sleep.

Description

A kind of beverage and preparation method thereof for improving mood

Technical field

The invention belongs to field of food, are related to a kind of instant tea, and in particular to a kind of beverage for improving mood and its Preparation method.

Background technique

Instant tea powder is a kind of solid drink tea that can dissolve in water.With made tea, semi-finished product tea, tealeaves by-product Product or fresh tea leaves and herbaceous plant class, cereals etc. are raw material, produce work by extraction, filtering, purification, concentration, drying etc. Skill, that is processed into a kind of is dissolved in water and crystal grain shape, powdered or strip new beverage without tea grounds.

Instant tea it have drink conveniently (adding water i.e. drinkable), no rubbish, relative volume (or weight) is small, fortune easy to maintain It is defeated, be free of harmful substance^[2]；Contain the nutritional ingredient and flavor substance that millet paste is able to enter in traditional tea；With tea to human body All effects, it is currently in the world, especially rather popular in American-European countries, be a kind of very promising drink.China's speed Molten tea development work starts from the early 1960s, being developed so far, and production of instant tea has begun to take shape, and Product Marketing is external, deep By the favorable comment of foreign trader and overseas Chinese.

The research and development of instant tea powder experienced continuous development, the process constantly improve, in product category, product quality and product Sale etc. makes a breakthrough.Currently, there are about more than 50 for the provinces and cities such as Fujian, Zhejiang, Hunan, Shenzhen, Jiangxi, Jiangsu Plant produced instant tea powder and its series of products (seasoning class, herbaceous plant class, functional form instant tea powder etc.), China's instant tea powder Production constantly develop.Tea beverage, tealeaves carbonated drink, tealeaves sugar, tealeaves ice cream, tealeaves chewing gum, cakes and sweetmeats product at present, And as natural food additive, nearly 40% market share is occupied, and China's tea beverage is every year with 30% speed Increase, the instant tea powder demand of beverage is also correspondingly increased with annual 3%~5% speed.

In the development to bealth-care instant tea, Ruan Meijuan thinks, using tea and hawthorn as the instant plain tea of raw material and instant mountain Short, bristly hair or beard tea production technology, manufactured goods be it is light yellow, quality is loose, the strongly fragrant dense powder of tea perfume, have good brew.Instant mountain The healthcare function and one of short, bristly hair or beard tea collection hawthorn and tea are that a kind of healthcare function is strong, solid beverage of unique flavor.In research eight treasures (choice ingredients of certain special dishes) When instant tea, Bai Weidong etc. is pointed out, eight treasures (choice ingredients of certain special dishes) instant tea be after assorted, extracted, be concentrated using black tea as raw material, and add longan, The extract of jujube, chrysanthemum, lichee etc., through graininess instant solid beverage made of granulating and drying.With good hot melt with Cold soluble, it had not only improved the color of tea, but also increased the health of tea, there is good development prospect.

As a kind of commodity of new development, instant tea is fast-developing in many countries and regions in recent years and comes into vogue, Due to it is a kind of drink of health, have the advantages that convenient, healthy, quick.The processing technology of external instant tea has become In maturation, and China is in development period, and the instant tea of China's production at present is lacked competitiveness in the international market, main cause Be flavour it is light, it is cold soluble it is poor, fragrance is low, sale and the quality problems such as the storage in process of consumption, packaging, this is still instant from now on Tea, iced tea, the developmental research of liquid tea beverage and the upper problems faced of production.The kind analogy of tealeaves functional component novel foodstuff at present More single, market scale is still smaller, and industrialization level is not high, and the ability of resisting risk is weaker, lacks product diversity and market Flexibility is not able to satisfy the market demand of different hierarchies of consumption.

Recognized by the research institution of many countries using the raw material of instant tea powder as drug, cosmetics, antioxidant Together.Therefore, it is raw material using tealeaves functional components, tea juice concentrating, instant tea powder, can develops with the natural of high added value The end products such as drug, personal care articles, functional form instant tea powder, healthy food mention for multiple functions type instant tea powder product Infinite commercial opportunities have been supplied, wide development prospect is provided with.

Under the social background of the burden of high pressure height instantly, the emotional problem of people is increasingly taken seriously, depression hair Sick rate increases year by year, and psychological problem will affect physiology, and the generation of these problems is not stranghtforward, small emotional problem Shi Keneng pays no attention to, but accumulation will develop as disease in the future, the physical and mental health of people be seriously endangered, so we should be first from every It has a good mood to start, and currently on the market with health-care efficacy instant tea mostly with weight-reducing, lipid-loweringing, build up health Improve traditional physiological function, based on the sub-health state for adjusting body, there is no be in a very depressed state for modern, be dysthymia, The drinks for the problems such as appetite is poor, sleep is poor.

Summary of the invention

To solve the above-mentioned problems, the purpose of the present invention is to provide a kind of for improving the beverage of mood, and the beverage is easy It is dissolved in water, belongs to instant tea type, is easy to brew, there is resolving stagnation for tranquilization, improvement is depressed, improves the effect of sleep.

Another object of the present invention is to provide a kind of preparation methods of above-mentioned beverage.

To achieve the goals above, the present invention provides a kind of for improving the beverage of mood, by as follows at being grouped as: safranine Flower 0.01-0.02 parts by weight, rose 0.5-1.5 parts by weight, bitter orange flower 0.5-1.5 parts by weight, arillus longan 0.5-1.5 weight Part.

The dosage form that the present invention provides the above-mentioned beverage is capsule or oral solution.

The present invention also provides the preparation methods of above-mentioned beverage, include the following steps:

1) safflower is mixed with two taste medicinal material of rose, beating powder to particle size range is 20~80 mesh, mixture I is made, It is spare；

2) arillus longan is dry to moisture < 8%, be added bitter orange flower use equivalent gradually-increased room temperature beat powder to particle size range for The mixture II of 20~80 mesh；

3) mixture I is uniformly mixed with mixture II, is made and brews mixture.

Brew mixture can directly boiled water brew reference, can also be further processed into be suitable for carry form.

Preferably, further comprise, step 4): this being brewed into mixture and is fitted into coffee capsule softgel shell, seal, packaging is Can, capule size can be 3g/.

Or, further comprising, step 4): mixture addition will be brewed and be equivalent to 8-12 times of recipe quantity 80 ± 5 DEG C of heat measured In water, temperature extraction takes 25min, filters, discards filter residue, cooling, and purified water is added to be settled to every bottle of corresponding crude drug amount 1.5-4.6g, then Secondary filtering, 135-137 DEG C of ultra high temperature short time sterilization 15-20s is filling, again 135-137 DEG C of ultra high temperature short time sterilization 15-20s, Oral solution is made.

Wherein, the step 2) drying is vacuum drying or freeze-drying.

Preferably, the vacuum drying condition are as follows: 50 DEG C~60 DEG C, vacuum degree 0.08~0.1MPa, 20h.

Preferably, the freeze-drying condition are as follows: -40 DEG C, setting time 60min of freezing control, retention time 90min；10 DEG C of primary drying set temperature, setting time 180min, retention time 1800min；Parsing-desiccation set temperature 20min, setting time 60min, retention time 360min.

Preferably, the operating method that the step 2) equivalent is progressively increased are as follows: same volume is added in arillus longan after the drying The bitter orange flower of amount, beats powder, screens out the mixed powder of arillus longan and bitter orange flower；Unsifted arillus longan bulky grain, adds same volume The bitter orange flower mixing of amount continues to play powder, sieving；Finally, remaining bitter orange flower mixing, which is added, in unsifted arillus longan bulky grain beats powder, Sieve is crossed until complete, and arillus longan and bitter orange flower mix three times to be beaten the partial size of powder and require and be sieving through 20~80 mesh, and arillus longan is made With the mixture II of bitter orange flower.

Arillus longan is high, viscous containing sugared part, is not easy to beat powder, single beats powder and easily clings pulverizer blade, can not rotate.The present invention In the first dry special processing method for beating powder again is used for arillus longan: it is first that arillus longan is dry, first remove moisture removal, then control System beats powder temperature, we in such a way that equivalent is progressively increased, are carried out beating powder, be efficiently solved with dried arillus longan and bitter orange flower The problem of viscous blade.

The present invention handles material by freeze-drying and vacuum drying mode respectively.

Wherein it is freeze-dried key parameter: -40 DEG C, setting time 60min, retention time 90min of freezing control；It is primary dry 10 DEG C, setting time 180min, retention time 1800min of dry set temperature；Parsing-desiccation set temperature 20min, setting time 60min, retention time 360min；

Wherein it is dried in vacuo key parameter: 50 DEG C~60 DEG C of drying temperature, 0.08~0.1MPa of vacuum degree, drying time 20h。

Comparative result: vacuum drying arillus longan is stronger, freeze-drying it is more crisp, but be dried in vacuo speed faster. Two methods can reach drying purpose.

Powder is beaten after arillus longan is dry, has investigated two ways: low-temperature grinding and ambient ground.Wherein low-temperature grinding (parameter: Instrument amplitude 6mm, -20 DEG C of temperature, grinding time 10min) after arillus longan due to sugar content height, largely attach to machine On, only small part is recycled, and will be cohered into one piece under the arillus longan powder room temperature of recycling, be failed to efficiently separate.Therefore Ambient ground mode is used in the present invention, i.e., carries out beating powder with equivalent addition with dried arillus longan and bitter orange flower, crush effect Fruit is preferable, is avoided that viscous knife problem.

Safflower used also known as west safflower, safron in the present invention, originate in Spain, Greece, southern Europe various countries and she The safranine Huadu on the ground such as bright, the world 80% originates from Iran.Also there are cultivation in India, Japan, are passed to China Tibet through India, thus claim For safflower, there is cultivation on the Tibet in China, Zhejiang, Jiangsu, Shandong, Beijing and other places.Safflower is also largely used to daily use chemicals, food The industries such as product, dyestuff, enjoy the three worlds most: most expensive medicinal plant, best dyestuff, most high-grade fragrance, by western class Tooth people is described as " red gold ".Shanghai Chongming plants safflower since the beginning of the eighties in last century, is the safflower of Largest In China Planting site, wherein 90% safflower originates in this place.The expensive traditional invigorant of safflower generic name, is entered with the style and column cap of gynoecium Medicine, traditional medicine ancient books, which records safflower, has the effect of activating microcirculation and removing stasis medicinal, swelling and pain relieving, removing pattogenic heat from the blood and toxic material from the body, resolving stagnation for tranquilization, can be used for The diseases such as Jing Bi Disorder lump in the abdomen, postpartum stasis, febrile virulent maculae, melancholy ruffian are bored, palpitation with fear is gone mad.Modern pharmacology research shows that safflower has The effect of keeping fit and healthy.More and more Scientific Articles confirm that safflower has treatment mental disorder, nervus retrogression disease Disease, learning memory disorder, cardiovascular disease, atherosclerosis, hyperlipidemia, diabetes, hypertension, gastric ulcer, fatty liver And the various actives such as anti-epileptic, anticonvulsion.Safflower is in Christian era in external earliest record as medicinal plant safflower Among 1550 or so " Ai Baisi sudd books ".Safflower has unique curative effect in terms of adjusting mood, improving, It there is no the drinks using safflower as core material currently on the market.

Rose acrid flavour, sweet, slightly warm in nature.Regulating the flow of vital energy and dispeling melancholy, promoting blood circulation to remove blood stasis.Rose adjustable mood but also can not only improve Sleep, for liver-stomach disharmony, hypochondriac pain gastral cavity is bored, epigastric distending pain；Irregular menstruation, premenstrual swollen breasts；Traumatic injury, pain caused by ecchymoma etc. All effectively.Bitter orange flower alias blood teacher, ochre, cold nature, bitter, acid, effect ventilating eliminate indigestion and phlegm, bored for chest and abdomen swollen Bitterly, accumulation of food in the stomach and intes tine due to indigestion, phlegm retention, rectal prolapse are effective, and have effect of weight reducing.The two can all cooperate safflower to play regulating the flow of vital energy and dispeling melancholy tranquilizing the mind Effect, rose can cooperate safflower to increase the effect of vital energy regualting and blood circulation-promoting, solve dysthymia crowd and be in a very depressed state, uncomfortable in chest not easypro Problem, while can be with activating microcirculation and removing stasis medicinal, the problems such as dim complexion, dysmenorrhea caused by qi depression to blood stasis, bitter orange flower can cooperate safflower, rose Rare flower solves to digest the problems such as poor after food since the gloomy caused appetite of mood is poor.Arillus longan sweet, warm.Heart-spleen boosting fills blood, Calm the nerves, with palpitaition, amnesia, diarrhea, the oedema etc. for controlling heart and spleen deficiency；Insufficiency of vital energy and blood and induced insomnia, metrorrhagia and metrostaxis, menstrual vertigo；Lung yin Virtual loss, lung lose profit drop and dry cough, phlegm are caused to stick white, hoarseness, dry mouth and throat etc. less.The work of the heart-spleen boosting of arillus longan, qi and blood invigorating With the above vital energy regualting and blood circulation-promoting medicine can be cooperated, it can prevention and treatment vital energy regualting and blood circulation-promoting too caused by gas consumption blood trouble, and can with nourishing blood and tranquilization, Sleep disturbance caused by improving because of mood pent-up, can also adjust mouthfeel.Therefore the beverage compatibility mend and rush down and combine, relieve stagnation peace is played altogether Mind, nourshing blood and promoting blood circulation, the function of resolving phlegm and dissipating blood stasis, four traditional Chinese medicine object come under integration of drinking and medicinal herbs, are suitble to take for a long time.

The preferred oral solution of dosage form or capsule in the present invention, wherein capsule is coffee capsule form.

For coffee capsule from capsule, English meaning is exactly capsule, although drink, has been risen a as medicine Name.But this name is really lively the characteristics of reflecting coffee capsule, as conventional capsule and colloid packaging In contain flour.

But also there is certain difference therebetween, from capsule itself: coffee capsule volume is big, and shell is by aluminium foil, modeling The materials such as material are made, and conventional capsule is small in size, and shell is made of materials such as gelatin, celluloses；Powder is filled in coffee capsule Matter, conventional capsule fill powdery or liquid substance；Coffee capsule generally fills the substances such as instant coffee, tea, conventional capsule filling Drug, health food；Coffee capsule is in a circular table shape, and conventional capsule is cylindrical；Coffee capsule is rushed using coffee capsule making device Bubble, filler discharge in coffee capsule making device, and conventional capsule is directly swallowed, and content is discharged in gastrointestinal tract.

From fill process: coffee capsule: coffee capsule is the arrangement of capsule shells, is calibrated, filling, the process of sealer. Conventional capsule: soft capsule is filmogen and content simultaneous processing into product；The production of hard capsule is Capsules shell Arrangement, calibration, the process of separation and filling fitting.

The benefit of coffee capsule is, because capsule wall quality is more hard, keeps prototype well at high temperature, therefore can To inject high-pressure water vapor in capsule, so that filler be made to analyse completely under the effect of the pressure, can preferably guarantee so interior The precipitation of tolerant ingredient.

The form of coffee capsule can be used in the beverage of offer of the invention, due to the preparation process according to coffee capsule, Inert gas is filled in capsule, can effectively extend the shelf lives of beverage provided by the invention, and is had and to be sterilized again Effect.The beverage can be made into the capsule form for being suitble to various coffee capsule making devices to use, by coffee capsule making device by the list infused coffee One Enlargement is to instant tea field.

The dosage form for the oral solution that the present invention uses can directly be taken, and without being brewed again, be more suitable for outgoing etc. Lack the environment that hot water or capsule machine brew, convenience and sanitation for drinking.

The beneficial effects of the present invention are:

1, beverage provided by the invention first present invention employs oral solution or makes similar coffee compared with traditional tea-drinking The technique of coffee capsule, both methods, which can effectively seal original effective component and smell in formula up for safekeeping, to be lost, and can The requirement of a variety of environment such as facilitate routine office work, go on business.

2, traditional instant tea adds a certain amount of sucrose or white granulated sugar flavor adjustment more, but unsuitable diabetes patient It takes for a long time.Beverage provided by the invention does not add sugar additionally, with the sugar flavor adjustment of arillus longan itself, compares addition Sucrose or white granulated sugar, more healthy, safety.

3, the prescription of the four traditional Chinese medicine object in beverage provided by the invention is relatively simple, and but compatibility is reasonable, mend and rush down and combine, mutually auxiliary It coordinates, can play and adjust mood, improve dysthymia, improve the multiple efficacies such as poor, appetite is poor, dysmenorrhea of sleeping, four traditional Chinese medicine object is more It is integration of drinking and medicinal herbs, it is very safe.The formula both considered the function taken, it is contemplated that the mouthfeel taken, is suitble to height The long-term use of pressure duty life crowd.There is no currently on the market with the components compatibilities such as safflower at for modern's mood It is depressed, dysthymia, appetite is poor, sleep difference the problems such as drinks.The present invention is to adjust based on the sub-health state of mood Function is wanted, improves the dysthymic mood of people and its caused related discomfort, prevents it and be evolved into heavier mental disease.

Detailed description of the invention

Fig. 1 is the result figure that beverage provided by the invention moves vertically before modeling in rat pharmacodynamic experiment.

Fig. 2 is the result figure that beverage provided by the invention moves vertically after modeling in rat pharmacodynamic experiment.

Fig. 3 is the result figure of beverage provided by the invention horizontal movement before modeling in rat pharmacodynamic experiment.

Fig. 4 is the result figure of beverage provided by the invention horizontal movement after modeling in rat pharmacodynamic experiment.

Fig. 5 is the result figure of beverage provided by the invention total distance before modeling in rat pharmacodynamic experiment.

Fig. 6 is the result figure of beverage provided by the invention total distance after modeling in rat pharmacodynamic experiment.

Fig. 7 is the result figure of beverage provided by the invention modeling front overhang tail dead time in rat pharmacodynamic experiment.

Fig. 8 is the result figure of beverage provided by the invention modeling rear overhang tail dead time in rat pharmacodynamic experiment.

Fig. 9 is the result figure of the beverage forced swimming dead time before modeling in rat pharmacodynamic experiment provided.

Figure 10 is the result figure of the beverage forced swimming dead time after modeling in rat pharmacodynamic experiment provided.

Specific embodiment

Embodiment 1

Safflower 0.13g, rose 6g, bitter orange flower 8g, arillus longan 7g.

The preparation method comprises the following steps:

1) safflower is mixed with two taste medicinal material of rose, beats powder to 40 mesh of partial size, mixture I is made, spare；

2) arillus longan vacuum drying (50 DEG C, vacuum degree 0.08MPa) is added bitter orange flower progress equivalent and progressively increases to moisture < 8% Beat the mixture II of powder to 40 mesh of particle size range；

3) mixture I is uniformly mixed with mixture II, is made and brews mixture；

4) it brews mixture to be fitted into coffee capsule softgel shell, seal, packaging.Specification is 3g/.

Embodiment 2

Safflower 0.18g, rose 14g, bitter orange flower 12g, arillus longan 13g

The preparation method comprises the following steps:

1) safflower is mixed with two taste medicinal material of rose, beats powder, mixture I is made in 40 mesh of partial size, spare；

2) by arillus longan vacuum drying (55 DEG C, vacuum degree 0.1MPa) to moisture < 8%, bitter orange flower progress equivalent is added and passs Add the mixture II for beating powder to 40 mesh of particle size range；

3) mixture I is uniformly mixed with mixture II, is made and brews mixture；

4) mixture will be brewed to be added in 80 DEG C of hot water for being equivalent to 10 times of formula ratio amounts, temperature extraction takes 25min, mistake Filter, discards filter residue, cooling, adds purified water to be settled to 0.09g/mL, filters again, 135 DEG C of ultra high temperature short time sterilization 20s, filling, 135 DEG C of ultra high temperature short time sterilization 20s again, are made oral solution.

3 long term toxicity test of embodiment

Rat is bought from Beijing Vital River Experimental Animals Technology Co., Ltd..

Animal；SPF grades of SD male rats, weight 200g-210g, week old: 7 weeks, 24.

Environment temperature is (24 ± 1) DEG C, and humidity is (55 ± 5) %, after adaptable fed 3 days.

1.1 experimental procedures:

It is randomly divided into control group and beverage group, every group each 12.Control group stomach-filling drinking water, beverage group use embodiment 2 Formula, according to implementing 2 method and steps 3) brewing for preparation be directly dissolved in purified water preparation stomach-filling liquid after mixture, according to per capita Weight 70kg, drinking 150mL oral solution daily and calculating the daily dosage of 200-210g/ rat is about crude drug 0.48-0.5g, folding 0.24g/mL/100g weight is closed, stomach-filling liquid is prepared, to detect formula toxicity.

Beverage group gastric infusion, 1 time a day, continuous 28 days, dosage 1mL/100g, administration concentration 0.24g/ mL.Control group stomach-filling drinking water, once a day, continuous 28 days, dosage 1mL/100g.Administration 28 days, rats by intraperitoneal injection 4% Chloraldurate 1g/100g anesthesia, take blood abdominal aortic blood after anesthesia, carry out the inspection of blood routine, liver kidney function；Coring, liver, The weighing of the important organs such as spleen, lung, kidney, stomach, testis, calculates organ coefficient；It takes liver, kidney to immerse in formalin, does pathology Slice, whether there is or not pathological changes for observation.

1.2 experimental results:

1) to the influence of blood routine:

Continuously take beverage 28 days as shown in table 1, compared with the control group, no statistics is poor for the blood routine indices of rat Different, beverage provided by the invention has no significant effect WBC, RBC, HGB, PLT, NEUT, LYMP, MONO of rat.

The comparison of the blood routine of 1 beverage of table and control group

As it can be seen from table 1 taking the blood routine indices and control group of rat after beverage four weeks provided by the invention Without significant difference, beverage provided by the invention is to blood routine WBC, RBC, HGB, PLT, NEUT, LYMP, MONO of rat without obvious It influences.

2) to the influence of liver function:

The liver function in rat blood serum is measured using automatic clinical chemistry analyzer, as shown in table 2, continuously takes this Beverage 28 days of invention offer, compared with the control group, no difference of science of statistics, the beverage is to rat for the liver function indices of rat ALT, AST, TBIL, ALP, TO, ALB, G have no significant effect.

The comparison of the liver function of 2 beverage of table and control group

From table 2 it can be seen that taking the liver function indices and control group of rat after beverage four weeks provided by the invention Without significant difference, which has no significant effect ALT, AST, TBIL, ALP, TO, ALB, G of rat.

3) influence to renal function, blood lipid and blood glucose:

Renal function, blood lipid and the blood glucose in rat blood serum are measured using automatic clinical chemistry analyzer, such as 3 institute of table Show, continuously take the beverage 28 days, compared with the control group, no statistics is poor for the renal function indices of rat, blood glucose, blood lipid Different, which has no significant effect UREA, BUN, CREA, UA, GLU, TCH of rat.

The comparison of the renal function, blood lipid, blood glucose of 3 beverage of table and control group

From table 3 it can be seen that take the renal function, blood lipid, blood glucose indices and control group of rat after beverage four weeks without Significant difference, beverage have no significant effect UREA, BUN, CREA, UA, GLU, TCH of rat.

4) to the influence of organ index:

After rat is put to death, solution takes the weighing of its important organ, and calculates internal organs with organ weights/rat body weight * 100 and refer to Number.As shown in table 4, continuously take beverage 28 days, the important organs such as the heart, liver, spleen, lung, kidney, stomach, testis of rat index with it is right It is compared according to group, no difference of science of statistics, beverage has no significant effect the important organs such as the heart of rat, liver, spleen, lung, kidney, stomach, testis.

The comparison of 4 beverage of table and control group important organ index

Refer to from table 4, it can be seen that taking the renal function, blood lipid of rat, blood glucose items after beverage four weeks provided by the invention Without significant difference, beverage has no significant effect the important organs such as the heart of rat, liver, spleen, lung, kidney, stomach, testis for mark and control group.

1.3 experiment conclusions:

It can be obtained by the result of above-mentioned long term toxicity test, beverage provided by the invention is continuously taken 28 days, to the blood of rat Routine, liver function, renal function, blood lipid, blood glucose are influenced without apparent, to important organs such as the heart, liver, spleen, lung, kidney, stomach, testis Index all has no significant effect, and beverage oral administration illustrates beverage safety provided by the invention without apparent toxic side effect, nontoxic Side effect.

4 pharmacodynamic experiment of embodiment

Rat is bought from Beijing Vital River Experimental Animals Technology Co., Ltd..

Animal；SPF grades of SD male rats, weight 200g-210g, week old: 7 weeks, 40.

Drug: reserpine injection (1mg/1mL), fluoxetine HCl capsules (Suzhou Li Lai pharmaceutical Co. Ltd, specification: 20mg), aqueous beverage solution.

Equipment: spacious field experimental box, forced swimming bucket.

Spacious field experiment is also known as Open field test, is evaluation experimental animal independent behaviour, exploratory behavior and tight in strange environment A kind of method of tonicity.With experimental animal, the occurrence frequency of certain behaviors and duration etc., reaction are real among novel environment Test independent behaviour and exploratory behavior of the animal in foreign environment.

Horizontal movement is the lattice number that rat crosses in spacious field activity, and vertical movement is that rat lifts two in spacious field activity The number of a forelimb, the summation of both of which score are exactly total distance.

Tail-suspention test (Tail suspension test, TST) be it is a kind of classical and can Fast Evaluation antidepressant, The method of analeptic drug, downern drug effect.Its principle is to be escaped but can not be escaped using attempt after rat tail suspension, to put It abandons and struggles, record the animal dead time into the distinctive motionless state of depression, in experimentation to reflect depressive state, antidepression Drug, analeptic drug, which can significantly shorten, changes its state.

Forced swim test (Forced Swimming Test, FST) is mainly used for antidepression, calmness and analgesic class medicine The research of object.It is placed in the environment of a limitation (such as in water) by rat, rat risks one's life to struggle in this context and make an attempt at escaping It can not escape again, to provide one without avoidable compressing environment, after the experiment of a period of time, animal shows typical case " motionless state ", reflect one kind and be referred to as " behavioral despair state ", recorded in experimentation the animal dead time come it is anti- Depressive state is reflected, antidepressant, analeptic drug, which can significantly shorten, changes its state.

1.1 experimental animal modelings and administration:

1) environment temperature is (24 ± 1) DEG C, and humidity is (55 ± 5) %, after adaptable fed 3 days.

2) measurement, forced swim test, spacious field experiment, the outstanding motionless experiment of tail of weight are carried out after 3 days.Reject spacious field activity Rat on the high side and partially long the forced swimming time.

3) remaining to be randomly divided into 4 groups, respectively blank group, model group, positive controls, beverage group.

Beverage group uses the formula of embodiment 1, and capsule is not perfused, will directly brew made of step 3) mixture be dissolved in it is pure Change water and stomach-filling liquid is made, it is daily to be calculated using 5 capsules according to weight 70kg per capita, calculate 200-210g/ rat per daily Dose is about crude drug 0.48-0.5g, is converted into 0.24g/mL/100g weight, prepares stomach-filling liquid.

Blank group refuses any stimulation, normal water diet；Configured reserpine is injected intraperitoneally in remaining group rat daily Solution 0.2mg/kg, continuous 28 days；30min is administered before daily every rats by intraperitoneal injection, and every group of difference gastric infusion is continuously given Medicine 28 days.

Beverage group gastric infusion beverage, 1 time a day, continuous 28 days, dosage 1mL/100g, administration concentration was 0.24g/mL.Model group stomach-filling drinking water, 1 time a day, continuous 28 days, dosage 1mL/100g.Positive controls stomach-filling fluorine west Spit of fland solution, 1 time a day, continuous 28 days, dosage 1mL/100g, administration concentration 0.21mg/mL.

4) variation of weight is monitored weekly

5) 120min has been injected intraperitoneally for the last time and has carried out behaviouristics detection, forced swim test, spacious field experiment, outstanding tail are not Dynamic experiment.

1.2 experimental result

1) to the influence of spacious field experiment

Vertical movement:

As shown in Figure 1, indifference before modeling between vertical movement each group, as shown in Fig. 2, after vertical movement modeling, model group Have an extremely significant statistical difference with blank group, model group and positive controls have an extremely significant statistical difference, model group and Beverage group has statistical difference.

Influence to horizontal movement:

As shown in figure 3, indifference before modeling between horizontal movement each group, as shown in figure 4, after horizontal movement modeling, model group Have an extremely significant statistical difference with blank group, model group and positive controls have an extremely significant statistical difference, model group and Beverage group has statistical difference.

Influence to total distance:

Fig. 5 shows between total distance each group indifference before modeling, after Fig. 6 shows total distance modeling, model group and blank group There is extremely significant statistical difference, model group and positive controls have extremely significant statistical difference, and model group and beverage group have Significant statistical difference.

Can be obtained by Fig. 1,3,5, before modeling each group rat spacious field experiment in vertical movement, horizontal movement and total distance it is equal No significant difference, it is seen that independent behaviour and exploratory behavior of each group in foreign environment are without difference between obviously group before modeling 's.

It can be obtained by Fig. 2,4,6, after modeling between blank group and model group in terms of vertical movement, horizontal movement and total distance There is extremely significant difference, vertical movement, horizontal movement and the total distance of model group significantly reduce, and illustrate blank group and model group Independent behaviour and exploratory behavior are variant in foreign environment, and model group rats show the guard significantly to foreign environment, The exploration to novel environment is reduced, a kind of state of depression is shown, prompts modeling success, the drug induced depression model of rat Success.

Occur extremely showing in terms of vertical movement, horizontal movement and total distance between model group and positive controls after modeling Difference is write, positive controls movement obviously increases, and prompts positive control medicine that can alleviate the depressed shape of rat to a certain extent State restores exploratory behaviour of the depression rat for foreign environment.

There is statistical difference in terms of vertical movement, horizontal movement between model group and beverage group after modeling, in total distance Aspect has significant difference, and beverage group move distance also increases compared with model group, prompts the beverage that can alleviate to a certain extent The depressive state of rat restores exploratory behaviour and independent behaviour of the depression rat for foreign environment.Confirm the beverage for The improvement of depression rat is depressed, alleviates the effect of depression.

2) to the influence of outstanding tail dead time

Fig. 7 shows between outstanding tail dead time each group indifference before modeling, after Fig. 8 shows outstanding tail dead time modeling, mould Type group and blank group have statistical difference, model group and positive controls no difference of science of statistics, and model group and beverage group are without statistics Learn difference.

It can be seen from figure 7 that before modeling the outstanding tail dead time of each group rat be not have discrepant, therefore baseline is flat Weighing apparatus.

It can be obtained by Fig. 8, blank group and model group are variant after modeling, illustrate that the dead time of rat tail suspension after modeling is obvious Extend, shows that the rat depressed dead time extends, illustrate modeling success, the success of rat drug depression model.

Model group and positive controls, beverage group do not have difference after modeling, illustrate positive control medicine and drink after modeling Material does not effectively shorten its depressed dead time, improves the rat state under desperate mode.

3) influence to the forced swimming dead time:

Fig. 9 shows that indifference, Figure 10 show the forced swimming dead time before modeling between forced swimming dead time each group After modeling, model group and blank group have significant statistical difference, and model group and positive controls have statistical difference, model group With beverage group no difference of science of statistics.

It can be obtained by Fig. 9, the forced swimming dead time of each group rat is that do not have discrepant, therefore baseline before modeling before modeling It is balance.

It can be obtained by Figure 10, blank group and model group have significant difference after modeling, illustrate rat forced swimming after modeling Dead time is obviously prolonged, and shows that the rat depressed dead time extends, illustrates modeling success, the success of rat drug depression model.

Model group and positive controls are variant after modeling, and positive control medicine can shorten depression rat after illustrating modeling The motionless time in water can improve depressive state to a certain extent.

Model group and beverage group do not have difference after modeling, which does not effectively shorten its depressed dead time, Bu Nenggai Rat state under kind despair mode.

By above-mentioned experiment it can be concluded that model group and blank group, which compare every behavioral indexes, difference, therefore model group is made Mould is successful, the success of chronic depression model caused by reserpine.Positive control and the most of behavioral indexes of model group have difference, say This bright model can be treated by positive drug, further demonstrate model modeling success.Beverage group and model group are tested in spacious field Difference is shown, illustrates that the beverage can improve depression to a certain extent, increase and explore to foreign environment, changing in behavioral despair It is apt to unobvious.Beverage provided by the invention improves depressive state to a certain extent, can improve mood after taking really.

From above-described embodiment as can be seen that the Chinese medicine compatibility provided by the invention with a variety of integration of drinking and medicinal herbs such as safflower, It is made up of exclusive formulation and technology, the pre- making drinks of the independent outer packing of a variety of flower compositions have resolving stagnation for tranquilization, slow down pressure The effect of power, improvement is depressed, improves sleep, beverage of the invention is using oral solution or the dosage form of coffee capsule, side Portable belt is drunk at any time convenient for daily, and capsule form has also expanded the application range of coffee capsule making device.

Claims (9)

1. a kind of for improving the beverage of mood, which is characterized in that by as follows at being grouped as: safflower 0.01-0.02 parts by weight, Rose 0.5-1.5 parts by weight, bitter orange flower 0.5-1.5 parts by weight, arillus longan 0.5-1.5 parts by weight.

2. beverage as described in claim 1, which is characterized in that the dosage form of the beverage is capsule or oral solution.

3. a kind of preparation method of beverage as described in claim 1, which comprises the steps of:

1) safflower is mixed with two taste medicinal material of rose, beating powder to particle size range is 20~80 mesh, and mixture I is made, spare；

2) arillus longan is dry to moisture < 8%, be added bitter orange flower use equivalent gradually-increased room temperature beat powder to particle size range for 20~ The mixture II of 80 mesh；

3) mixture I is uniformly mixed with mixture II, is made and brews mixture.

4. the preparation method of beverage as claimed in claim 3, which is characterized in that further comprise, step 4): this is brewed mixing Material is fitted into coffee capsule softgel shell, is sealed, packaging.

5. such as the preparation method of beverage as claimed in claim 3, which is characterized in that further comprise step

4): mixture addition will be brewed and be equivalent in 80 ± 5 DEG C of hot water that 8-12 times of formula ratio is measured, temperature extraction takes 25min, mistake Filter, discards filter residue, cooling, adds purified water to be settled to every 50mL and corresponds to crude drug amount 1.5-4.6g, filters again, and 135-137 DEG C super High-temperature short-time sterilization 15-20s, filling, 135-137 DEG C of ultra high temperature short time sterilization 15-20s, is made oral solution again.

6. the preparation method of beverage as described in claim any one of 3-5, which is characterized in that the step 2) drying is dry for vacuum Dry or freeze-drying.

7. the preparation method of beverage as claimed in claim 4, which is characterized in that the vacuum drying condition are as follows: 50 DEG C~60 DEG C, Vacuum degree 0.08~0.1MPa, 20h.

8. the preparation method of beverage as claimed in claim 4, which is characterized in that the freeze-drying condition are as follows: freezing control - 40 DEG C, setting time 60min, retention time 90min of system；It 10 DEG C of primary drying set temperature, setting time 180min, keeps Time 1800min；Parsing-desiccation set temperature 20min, setting time 60min, retention time 360min.

9. such as the preparation method of the described in any item beverages of claim 3-5, which is characterized in that the step 2) equivalent is progressively increased Operating method are as follows: the bitter orange flower of same volume accumulated amount is added in arillus longan after the drying, beats powder, screens out arillus longan and bitter orange flower Mixed powder；Unsifted arillus longan bulky grain adds the bitter orange flower of same volume accumulated amount to mix and continues to beat powder, sieving；Finally, non-mistake The arillus longan bulky grain of sieve is added remaining bitter orange flower mixing and beats powder, until complete cross sieve, arillus longan and bitter orange flower mix beat three times The partial size of powder, which requires, is sieving through 20~80 mesh, and the mixture II of arillus longan and bitter orange flower is made.