CN109142008A - A kind of fixer colouring pathological tissue specimen - Google Patents
A kind of fixer colouring pathological tissue specimen Download PDFInfo
- Publication number
- CN109142008A CN109142008A CN201811007215.0A CN201811007215A CN109142008A CN 109142008 A CN109142008 A CN 109142008A CN 201811007215 A CN201811007215 A CN 201811007215A CN 109142008 A CN109142008 A CN 109142008A
- Authority
- CN
- China
- Prior art keywords
- fixer
- tissue specimen
- pathological tissue
- colouring
- liquid device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000001575 pathological effect Effects 0.000 title claims abstract description 37
- 238000004040 coloring Methods 0.000 title claims abstract description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 30
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 10
- 229910000397 disodium phosphate Inorganic materials 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 10
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 10
- 229910000162 sodium phosphate Inorganic materials 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 18
- 238000000338 in vitro Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
- 230000003834 intracellular effect Effects 0.000 abstract description 3
- 238000003908 quality control method Methods 0.000 abstract description 3
- 238000010186 staining Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000003915 air pollution Methods 0.000 abstract description 2
- 239000003086 colorant Substances 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000010827 pathological analysis Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Abstract
The present invention relates to a kind of fixer for colouring pathological tissue specimen, the component for including and proportion are as follows: formalin solution: 90-130ml；Yihong: 0.015-0.025g；Na2HPO4·12H2O:14-17g；NaH2PO4·2H2O:4.4-4.7g;NaCL:8-10g；Acetic acid: 27-32ml.The present invention can make pathological tissue specimen coloring be easy to find and identify, can improve the fixed effect of pathological tissue, reduce the influence to intracellular reactive substance, improve the accuracy of slice.This product is prepackage fixer simultaneously, without preparing, plug and play, it is easy to operate, it largely facilitates the operation sequence of lab assistant or medical staff, avoid air pollution, and play the role of standardization and standardized, the sample quality control before being conducive to staining analysis.
Description
Technical field
The present invention relates to a kind of fixers for colouring pathological tissue specimen, belong to sample disposal technical field.
Background technique
Pathology technique is the basis of pathological diagnosis, and modern pathological diagnosis is increasingly dependent on the development of pathology technique.With point
The fast development of sub- pathology technique, the requirement to tissue fixed effect are higher and higher.The quality of pathological section manufacturing technology is related to
Multiple links, wherein cell especially puncturing tissue has the phenomenon that can not being found and identifying during processing, causes certain
A little serious consequences.
Traditional cell is wrapped up commonly using fibrous paper etc., but while will appear bad tissue dewatering, waxdip is difficult to seep
Thoroughly etc..
Summary of the invention
The technical problem to be solved by the present invention is in view of the above-mentioned drawbacks in the prior art, provide a kind of instant, solid
Determine the fixer that effect is good and puncturing tissue coloring can be made to find and identify convenient for tissue.
In order to solve the above technical problems, a kind of fixer for colouring pathological tissue specimen provided by the invention, special
Sign is that the component for including and proportion are as follows:
Formalin solution: 90-130ml；
Yihong: 0.015-0.025g；
Na2HPO4·12H2O:14-17g；
NaH2PO4·2H2O:4.4-4.7g；
NaCL:8-10g；
Acetic acid: 27-32ml.
As further embodiment, it is described make pathological tissue specimen colour fixer, the component for including and proportion it is as follows:
Formalin solution: 100-120ml；
Yihong: 0.018-0.025g；
Na2HPO4·12H2O:14-16g；
NaH2PO4·2H2O:4.4-4.6g；
NaCL:8.5-10g；
Acetic acid: 28-31ml.
As further scheme, the fixer for colouring pathological tissue specimen, the component for including and with such as
Under:
Formalin solution: 120ml；
Yihong: 0.02g；
Na2HPO4·12H2O:15g；
NaH2PO4·2H2O:4.5g；
NaCL:8.5g；
Acetic acid: 30ml.
The fixer that the present invention colours pathological tissue specimen also further characterized in that
1, the pH value 7.0 ± 0.2 for the fixer for colouring pathological tissue specimen.
2, the mass percent concentration of formaldehyde is 37%-40% in formalin solution.
Further, the invention further relates to the application method of the above-mentioned fixer for colouring pathological tissue specimen, features
It is: selects the specification of fixed liquid device according to puncturing tissue size when in use, unscrews the pipe lid of fixed liquid device, it will be in vitro
Puncturing tissue is immediately placed in fixed liquid device (in 10min), is completely submerged in puncturing tissue in fixer, screws bottle
Lid, set time 2-4h.
Compared with prior art, the beneficial effects of the present invention are:
The present invention can make pathological tissue specimen coloring be easy to find and identify, can improve the fixed effect of pathological tissue, reduction pair
The influence of intracellular reactive substance improves the accuracy of slice.This product is prepackage fixer is opened i.e. without preparing simultaneously
With, it is easy to operate, it largely facilitates the operation sequence of lab assistant or medical staff, avoid air pollution, and
Play the role of standardization and standardized, the sample quality control before being conducive to staining analysis.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, right With reference to embodiment
The technical solution of this patent is described in further details.
Embodiment 1
The present embodiment make pathological tissue specimen colour fixer, according to comprising component and proportion it is as follows: the Fu Er of 40% concentration
Malin's solution: 90-130ml, Yihong 0.015-0.025g, Na2HPO4·12H2O:14-17g, NaH2PO4·2H2O:4.4-
4.7g, NaCL:8-10g, acetic acid 27-32ml；The above ingredient mixes solution, as make tissue specimen colour fixer,
In vitro pathological tissue specimen is placed in fixer, for fixed and temporary storage pathological tissue specimen.
The fixer that the present embodiment colours pathological tissue specimen when in use, selects the fixation liquid device of 3ml, and unscrewing is solid
In vitro puncturing tissue is immediately placed in fixed liquid device (in 10min), fixer and tissue specimen by the pipe lid for determining liquid device
Volume ratio is not less than 4 times, is completely submerged in puncturing tissue in fixer, screws bottle cap, set time 2-4h.
Embodiment 2
The present embodiment make pathological tissue specimen colour fixer, according to comprising component and proportion it is as follows: the Fu Er of 37% concentration
Malin's solution: 100-120ml, Yihong 0.018-0.025g, Na2HPO4·12H2O:14-16g, NaH2PO4·2H2O:4.4-
4.6g, NaCL:8.5-10g, acetic acid 28-31ml；The above ingredient mixes solution, as the fixation for colouring tissue specimen
In vitro pathological tissue specimen is placed in fixer by liquid, for fixed and temporary storage pathological tissue specimen.
The fixer that the present embodiment colours pathological tissue specimen when in use, selects the fixation liquid device of 3ml, and unscrewing is solid
In vitro puncturing tissue is immediately placed in fixed liquid device (in 10min), fixer and tissue specimen by the pipe lid for determining liquid device
Volume ratio is not less than 4 times, is completely submerged in puncturing tissue in fixer, screws bottle cap, set time 2-4h.
Embodiment 3
The present embodiment make pathological tissue specimen colour fixer, according to comprising component and proportion it is as follows: the Fu Er of 40% concentration
Malin's solution: 120ml, Yihong 0.02g, Na2HPO4·12H2O:15g, NaH2PO4·2H2O:4.5g, NaCL:8.5g, acetic acid
30ml；The above ingredient mixes solution, and as the fixer for colouring tissue specimen, in vitro pathological tissue specimen is placed on
In fixer, for fixed and temporary storage pathological tissue specimen.
The fixer that the present embodiment colours pathological tissue specimen when in use, selects the fixation liquid device of 3ml, and unscrewing is solid
In vitro puncturing tissue is immediately placed in fixed liquid device (in 10min), fixer and tissue specimen by the pipe lid for determining liquid device
Volume ratio is not less than 4 times, is completely submerged in puncturing tissue in fixer, screws bottle cap, set time 2-4h.
The present invention can make pathological tissue specimen coloring be easy to find and identify, can improve the fixed effect of pathological tissue, subtract
Few influence to intracellular reactive substance, improves the accuracy of slice.This product is prepackage fixer simultaneously, without preparing, i.e.,
It opens and uses, it is easy to operate, it largely facilitates the operation sequence of lab assistant or medical staff, avoid air dirty
Dye, and play the role of standardization and standardized, the sample quality control before being conducive to staining analysis.
In addition to the implementation, the present invention can also have other embodiments.It is all to use equivalent substitution or equivalent transformation shape
At technical solution, fall within the scope of protection required by the present invention.
Claims (8)
1. a kind of fixer for colouring pathological tissue specimen, it is characterised in that the component and proportion for including are as follows:
Formalin solution: 90-130ml；
Yihong: 0.015-0.025g；
Na2HPO4·12H2O:14-17g；
NaH2PO4·2H2O:4.4-4.7g；
NaCL:8-10g；
Acetic acid: 27-32ml.
2. the fixer according to claim 1 for colouring pathological tissue specimen, it is characterised in that the component that includes and match
Than as follows:
Formalin solution: 100-120ml；
Yihong: 0.018-0.025g；
Na2HPO4·12H2O:14-16g；
NaH2PO4·2H2O:4.4-4.6g；
NaCL:8.5-10g；
Acetic acid: 28-31ml.
3. the fixer according to claim 1 for colouring pathological tissue specimen, it is characterised in that the component that includes and match
Than as follows:
Formalin solution: 120ml；
Yihong: 0.02g；
Na2HPO4·12H2O:15g；
NaH2PO4·2H2O:4.5g；
NaCL:8.5g；
Acetic acid: 30ml.
4. it is according to claim 1 make pathological tissue specimen colour fixer, it is characterised in that: pH value be 7.0 ±
0.2。
5. the fixer according to claim 1-4 for colouring pathological tissue specimen, it is characterised in that: the good fortune
The mass percent concentration of formaldehyde is 37%-40% in your Malin's solution.
6. the application method for the fixer for colouring pathological tissue specimen described in claim 5, it is characterised in that: root when in use
The specification that fixed liquid device is selected according to puncturing tissue size unscrews the pipe lid of fixed liquid device, in vitro puncturing tissue is put into solid
Determine in liquid device, be completely submerged in puncturing tissue in fixer, screws bottle cap, set time 2-4h.
7. the application method of the fixer according to claim 6 for colouring pathological tissue specimen, it is characterised in that: fixed
Liquid device specification is 3ml.
8. the application method of the fixer according to claim 6 for colouring pathological tissue specimen, it is characterised in that: unscrewing
After the pipe lid of fixed liquid device, being put into for puncturing tissue is completed in 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811007215.0A CN109142008A (en) | 2018-08-31 | 2018-08-31 | A kind of fixer colouring pathological tissue specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811007215.0A CN109142008A (en) | 2018-08-31 | 2018-08-31 | A kind of fixer colouring pathological tissue specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109142008A true CN109142008A (en) | 2019-01-04 |
Family
ID=64825741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811007215.0A Pending CN109142008A (en) | 2018-08-31 | 2018-08-31 | A kind of fixer colouring pathological tissue specimen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109142008A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106323722A (en) * | 2015-06-30 | 2017-01-11 | 长春市致和生物科技有限公司 | Formula of tissue fixing liquid for coloring tissue and usage method thereof |
| CN107421796A (en) * | 2011-06-17 | 2017-12-01 | 罗氏血液诊断股份有限公司 | Solution for the tissue processing of biological sample |
| CN107478484A (en) * | 2010-11-10 | 2017-12-15 | 罗氏血液诊断股份有限公司 | Prepare the automated system and method for the biological sample for inspection |
-
2018
- 2018-08-31 CN CN201811007215.0A patent/CN109142008A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107478484A (en) * | 2010-11-10 | 2017-12-15 | 罗氏血液诊断股份有限公司 | Prepare the automated system and method for the biological sample for inspection |
| CN107421796A (en) * | 2011-06-17 | 2017-12-01 | 罗氏血液诊断股份有限公司 | Solution for the tissue processing of biological sample |
| CN106323722A (en) * | 2015-06-30 | 2017-01-11 | 长春市致和生物科技有限公司 | Formula of tissue fixing liquid for coloring tissue and usage method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王德田等: "《实用现代病理学技术》", 31 January 2012 * |
| 裴正礼: "《细胞超微结构基础》", 31 December 1986 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20190104 |