CN106323722A - Formula of tissue fixing liquid for coloring tissue and usage method thereof - Google Patents
Formula of tissue fixing liquid for coloring tissue and usage method thereof Download PDFInfo
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- CN106323722A CN106323722A CN201510380746.4A CN201510380746A CN106323722A CN 106323722 A CN106323722 A CN 106323722A CN 201510380746 A CN201510380746 A CN 201510380746A CN 106323722 A CN106323722 A CN 106323722A
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Abstract
The invention discloses a formula of tissue fixing liquid for coloring tissue and a usage method thereof. The invention aims at solving problems that small specimens in a process of slicing are not easy to discover and identify due to faded or shallow colors, after the small tissue specimens are prepared into wax blocks with eosin-containing tissue fixing liquid used in hospital at present. The invention discloses the reasonable formula of fixing liquid for coloring tissue and the usage method thereof, and main components comprise magenta, formalin, acetate acid gracial, alcohol, etc. The formula is suitable for pathological slide preparation of small specimens within 2mm. The fixing liquid has the characteristics of short specimen fixing time, good fixing effects, more stable coloring of tissue specimens, and the like, and specimens are easy to discover and identify.
Description
Technical field
The present invention relates to a kind of pathological tissue fixative, specifically one and make tissue coloring tissue fixative solution formula and using method thereof.
Background technology
The Hospitals at Present mode taked fixing to small sample coloring tissue within vitro tissue 2mm is dropping eosin stain in tissue fixative solution, small sample can be allowed to a certain extent to colour, the effect finding and identifying can be played before being manufactured into paraffin embedded tissues, and the tissue specimen coloured with Yihong, after forming wax stone through tissue dewatering, transparent, waxdip, embedding etc., Yihong color fading on part small sample or lighter, during tissue makes pathological section in wax stone, do not have the discovery to small sample and recognition reaction.
Summary of the invention
The present invention, in order to preferably find small sample within 2mm during pathological section and identify, through clinical trial for many years, adds biological dye azo-fuchsine in preparation tissue fixative solution.So that tissue specimen coloring is more stable, it is prone to find, identify in wax stone specimen carries out slicing processes.Improve accuracy and the film-making satisfaction of section.
The mode that the present invention realizes:
1 makes tissue colour tissue fixative solution formula: the 1) magenta of 0.5%, the formalin of 8-10%, 5% glacial acetic acid, 60-70% ethanol, and outward appearance be blueness to red liquid, pH value 6-7.
2) by biochemical investigation qualified products in being distributed into the most transparent plastic containers of 5-20ml, come into the market through vanning, instruct the scientific and reasonable use of medical care.
2 usings method
Fixing: 2mm fixes with inner tissue and i.e. can reach coloring effect in more than 20-30 minute.
Detailed description of the invention
Below by embodiment, the present invention is further described
Embodiment 1:
Taking out fresh gastric mucosa under gastroscope, put in tissue fixative solution more than 20-30 minute and i.e. reach fixed coloring effect, after pathology sample conventional treatment, in wax stone, tissue specimen coloring is substantially, it is easy to find to identify.
Embodiment 2
Under bronchoscope, take out fresh lung tissue, put in tissue fixative solution more than 20-30 minute and i.e. reach fixed coloring effect.After pathology sample conventional treatment, in wax stone, tissue specimen coloring is substantially, it is easy to find to identify.
Claims (2)
1. one kind makes tissue coloring tissue fixative solution formula and using method thereof, which is mainly characterized in that, one makes tissue coloring tissue fixative solution formula and using method thereof, formula is the magenta of 0.5%, the formalin of 8-10%, 5% glacial acetic acid, 60-70% ethanol, outward appearance is blue to red liquid, pH value 6-7.
2. using method described in claim 1 is, it is characterised in that 2mm fixes with inner tissue and within more than 20-30 minute, reaches fixed coloring effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510380746.4A CN106323722A (en) | 2015-06-30 | 2015-06-30 | Formula of tissue fixing liquid for coloring tissue and usage method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510380746.4A CN106323722A (en) | 2015-06-30 | 2015-06-30 | Formula of tissue fixing liquid for coloring tissue and usage method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106323722A true CN106323722A (en) | 2017-01-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510380746.4A Pending CN106323722A (en) | 2015-06-30 | 2015-06-30 | Formula of tissue fixing liquid for coloring tissue and usage method thereof |
Country Status (1)
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| CN (1) | CN106323722A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142008A (en) * | 2018-08-31 | 2019-01-04 | 江苏世泰实验器材有限公司 | A kind of fixer colouring pathological tissue specimen |
| CN111670896A (en) * | 2020-04-28 | 2020-09-18 | 浙江大学医学院附属妇产科医院 | Neutral buffer tissue fixing solution |
| IT201900023115A1 (en) * | 2019-12-05 | 2021-06-05 | Bio Optica Milano S P A | Solution and method for controlling the processing of a histological sample |
-
2015
- 2015-06-30 CN CN201510380746.4A patent/CN106323722A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142008A (en) * | 2018-08-31 | 2019-01-04 | 江苏世泰实验器材有限公司 | A kind of fixer colouring pathological tissue specimen |
| IT201900023115A1 (en) * | 2019-12-05 | 2021-06-05 | Bio Optica Milano S P A | Solution and method for controlling the processing of a histological sample |
| EP3832286A1 (en) | 2019-12-05 | 2021-06-09 | Bio-Optica Milano S.p.A. | Solution and method for controlling the processing of a histological sample |
| CN111670896A (en) * | 2020-04-28 | 2020-09-18 | 浙江大学医学院附属妇产科医院 | Neutral buffer tissue fixing solution |
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Legal Events
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Application publication date: 20170111 |