WO2009148549A3 - Novel biosensors for live cell imaging of intracellular proteins - Google Patents

Novel biosensors for live cell imaging of intracellular proteins Download PDF

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Publication number
WO2009148549A3
WO2009148549A3 PCT/US2009/003297 US2009003297W WO2009148549A3 WO 2009148549 A3 WO2009148549 A3 WO 2009148549A3 US 2009003297 W US2009003297 W US 2009003297W WO 2009148549 A3 WO2009148549 A3 WO 2009148549A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
intracellular proteins
mctimer
multifluorescent
monomeric
Prior art date
Application number
PCT/US2009/003297
Other languages
French (fr)
Other versions
WO2009148549A2 (en
Inventor
Peter Drain
Original Assignee
University Of Pittsburgh-Of The Commonwealth System Of Higher Education
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Pittsburgh-Of The Commonwealth System Of Higher Education filed Critical University Of Pittsburgh-Of The Commonwealth System Of Higher Education
Publication of WO2009148549A2 publication Critical patent/WO2009148549A2/en
Publication of WO2009148549A3 publication Critical patent/WO2009148549A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/655—Somatostatins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/507—Pancreatic cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/042—Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Endocrinology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

This invention is related to the field of imaging intracellular protein processing. Fluorescent probes capable of changing color over time are used to identify and track the synthesis, trafficking, and secretion of intracellular proteins. One such probe useful in this invention is a monomeric multifluorescent protein, specifically a mcTimer probe as a biosensor for secretory proteins. After expression, mcTimer sequentially changes from green to red over a twenty- four hour period. In particular, a monomeric multifluorescent protein is inserted into the C-linker portion of the insulin prepropeptide as a marker for normal insulin responses to glucose in cultured donor beta cells.
PCT/US2009/003297 2008-05-29 2009-05-29 Novel biosensors for live cell imaging of intracellular proteins WO2009148549A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5713108P 2008-05-29 2008-05-29
US61/057,131 2008-05-29

Publications (2)

Publication Number Publication Date
WO2009148549A2 WO2009148549A2 (en) 2009-12-10
WO2009148549A3 true WO2009148549A3 (en) 2010-01-28

Family

ID=41398714

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/003297 WO2009148549A2 (en) 2008-05-29 2009-05-29 Novel biosensors for live cell imaging of intracellular proteins

Country Status (1)

Country Link
WO (1) WO2009148549A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8232451B1 (en) 2011-02-15 2012-07-31 Yorktown Technologies, L.P. Blue transgenic fluorescent ornamental fish
US8232450B1 (en) 2011-02-15 2012-07-31 Yorktown Technologies, L.P. Purple transgenic fluorescent ornamental fish
US8975467B2 (en) 2012-06-12 2015-03-10 Yorktown Technologies, L.P. Green transgenic fluorescent ornamental fish
US9392776B2 (en) 2012-07-26 2016-07-19 Yorktown Technologies, L.P. Green transgenic fluorescent ornamental fish
US8987546B2 (en) 2012-11-06 2015-03-24 Yorktown Technologies, L.P. Orange transgenic fluorescent ornamental fish
US9380768B2 (en) 2014-01-31 2016-07-05 Yorktown Technologies, L.P. Red transgenic fluorescent ornamental fish
US9363986B2 (en) 2014-01-31 2016-06-14 Yorktown Technologies, L.P. Orange transgenic fluorescent ornamental fish
US9282729B2 (en) 2014-01-31 2016-03-15 Yorktown Technologies, L.P. Purple transgenic fluorescent ornamental fish
US9295238B2 (en) 2014-01-31 2016-03-29 Yorktown Technologies, L.P. Pink transgenic fluorescent ornamental fish
US9968077B2 (en) 2014-11-07 2018-05-15 Glofish Llc Blue transgenic fluorescent ornamental fish
KR101896610B1 (en) * 2017-02-24 2018-09-07 홍익대학교 산학협력단 Novel far-red fluorescent protein
EP4279901A1 (en) * 2022-05-17 2023-11-22 MEON Functional Diagnostics GmbH Method and apparatus for classifying white blood cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050196768A1 (en) * 2001-02-26 2005-09-08 Campbell Robert E. Monomeric and dimeric fluorescent protein variants and methods for making same
US7217789B2 (en) * 2000-06-14 2007-05-15 Clontech Laboratories, Inc. Fluorescent timer proteins and methods for their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217789B2 (en) * 2000-06-14 2007-05-15 Clontech Laboratories, Inc. Fluorescent timer proteins and methods for their use
US20050196768A1 (en) * 2001-02-26 2005-09-08 Campbell Robert E. Monomeric and dimeric fluorescent protein variants and methods for making same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BERTERA ET AL.: "Body window-enabled in vivo multicolor imaging of transplanted mouse islets expressing an insulin-Timer fusion protein.", BIOTECHNIQUES, vol. 35, no. 4, October 2003 (2003-10-01), pages 718 - 722 *

Also Published As

Publication number Publication date
WO2009148549A2 (en) 2009-12-10

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