CN109822705A - The colouring method of prothenchyma (of wood) and parenchyma cell in a kind of difference timber - Google Patents
The colouring method of prothenchyma (of wood) and parenchyma cell in a kind of difference timber Download PDFInfo
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- CN109822705A CN109822705A CN201910180766.5A CN201910180766A CN109822705A CN 109822705 A CN109822705 A CN 109822705A CN 201910180766 A CN201910180766 A CN 201910180766A CN 109822705 A CN109822705 A CN 109822705A
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000002023 wood Substances 0.000 title claims abstract description 26
- 238000004040 coloring Methods 0.000 title claims abstract description 21
- 238000004043 dyeing Methods 0.000 claims abstract description 50
- 238000005554 pickling Methods 0.000 claims abstract description 35
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000010828 elution Methods 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- 229910001753 sapphirine Inorganic materials 0.000 claims description 7
- 229960000583 acetic acid Drugs 0.000 claims description 6
- 239000012362 glacial acetic acid Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000000386 microscopy Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000000975 dye Substances 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 8
- 239000003086 colorant Substances 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 36
- 239000000243 solution Substances 0.000 description 24
- 235000019441 ethanol Nutrition 0.000 description 9
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 235000005456 Pinus sylvestris var mongolica Nutrition 0.000 description 5
- 241000114025 Pinus sylvestris var. mongolica Species 0.000 description 5
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 4
- 241000018646 Pinus brutia Species 0.000 description 4
- 235000011613 Pinus brutia Nutrition 0.000 description 4
- 241000018650 Pinus massoniana Species 0.000 description 4
- 235000008578 Pinus strobus Nutrition 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 240000007320 Pinus strobus Species 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 229920005610 lignin Polymers 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 235000005638 Austrian pine Nutrition 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 235000008565 Pinus banksiana Nutrition 0.000 description 2
- 241001236247 Pinus bungeana Species 0.000 description 2
- 235000013264 Pinus jeffreyi Nutrition 0.000 description 2
- 240000007263 Pinus koraiensis Species 0.000 description 2
- 235000011615 Pinus koraiensis Nutrition 0.000 description 2
- 235000011609 Pinus massoniana Nutrition 0.000 description 2
- 235000011610 Pinus tabuliformis Nutrition 0.000 description 2
- 235000008585 Pinus thunbergii Nutrition 0.000 description 2
- 235000014030 Podocarpus spicatus Nutrition 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 230000036755 cellular response Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000012137 double-staining Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
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- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 235000017985 rocky mountain lodgepole pine Nutrition 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- 241000218300 Liriodendron Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000992309 Strobus Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
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- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cosmetics (AREA)
- Coloring (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The step of the present invention provides the colouring methods of prothenchyma (of wood) and parenchyma cell in a kind of difference timber, are related to wood materials property studying technological domain, the colouring method includes: to be dyed with 1% sarranine dyeing liquor to Wooden slice, obtains sarranine stained slice；Gradient elution is successively carried out to sarranine stained slice with the ethanol water of mass percent concentration 30~70%, obtains de- stained slice；De- stained slice is cleaned with pickling solution, obtains pickling slice；Pickling is sliced with the A Li Xinlan dyeing liquor of mass percent concentration 1% and is dyed, the ethanol water elution of mass percent concentration 65~80%, mounting.The present invention dyes the sclerenchyma and parenchyma cell of Wooden slice using sarranine-A Li Xinlan, dye activity is improved by way of adjusting pH before the dyeing of A Li Xinlan, so that dyestuff effectively colours sclerenchyma, to obtain the dyeing effect of clarity height, good resolution, be conducive to wood materials Quality Research.
Description
Technical field
The present invention relates to prothenchyma (of wood)s in wood materials property studying technological domain more particularly to a kind of difference timber and thin
The colouring method of parietal cell.
Background technique
In the research of wood anatomy, in different cell or tissue levels, the microsection of high quality is necessary basis
Work.In microsection production at this stage, it to be either used for the paraffin section of observation, slip slice or semithin section, dye
Color is all committed step.In decades, has more report for the research of timber microsection colouring method.With coloring agent
Diversification, the colouring method that multiple coloring agents redye microsection is also more and more concerned.
The fast green double dye methods of sarranine-are most common double stainings in plant sections, and dyeing formality is simple and can be clearly
Show the structure of cell.It is fast green that China early in the organic section making handbook of nineteen sixty Jiangxi college of education has just had recorded sarranine-
Dual staining；1979 Nian Rongshou cypresses attempt to attempt the whole dye improvement of one step of dichromatism on its basis；Then very to scholar to sarranine-
Fast green double dyes have carried out reducing improvement that is time-consuming and improving dyeing effect.So far the fast green classical double dye methods of sarranine-extensive utilization
In all kinds of researchs.The fast green double dye fados of sarranine-distinguish biggish cell section for cytochemistry ingredient, and it is aobvious such as to make bark
Micro- slice is taken on a red color using the fast green double dye method microscopic examination lithocytes of sarranine-, make tension wood Liriodendron microsection when using kind
Gelatinous fiber content is high in red-fast green double greeny regions of dye method microscopic examination.And cytochemistry component difference is lesser thin
Born of the same parents' slice, the fast green classical double dye methods of sarranine-will appear not applicable situation.
Parenchyma cell is that the living cells type of the composition plant elementary organization of thin, the non-lignifying of a kind of cell wall has perhaps
More important function, such as photosynthesis, storage, secretion.Parenchyma cell is present in most plants body, and maturation is subsequent to renew work,
Only very thin primary wall without secondary wall, the generally almost equal polyhedron of diameter, but can also be divided into asterism, divide
Branch and arm etc..There are many function of parenchyma cell, such as hoard food, carry out photosynthesis and aerobic respiration.Prothenchyma (of wood)
It is the mechanical tissue in plant, is made of dead cell, plays a part of to reinforce plant.Cell wall uniform thickened, it is mainly wooden
The deposition of quality, also referred to as lignifying.
Since parenchyma cell and prothenchyma (of wood) are assembled by natural polymer, difficult to realize pair of conventional double-staining
The two distinguishes dyeing and observation, and the clarity and resolution ratio dyed in addition be not high, and the prior art lacks a kind of simple and effective
Distinguish the staining method of parenchyma cell and prothenchyma (of wood) in timber.
Summary of the invention
Existing colouring method is not clear enough to parenchyma cell in timber and prothenchyma (of wood) differentiation, differentiates in order to overcome by the present invention
The not high defect of rate provides a kind of colouring method for distinguishing prothenchyma (of wood) and parenchyma cell in timber.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides the colouring methods of prothenchyma (of wood) and parenchyma cell in a kind of difference timber, comprising the following steps:
(1) 45~75min is dyed to Wooden slice with the sarranine dyeing liquor of mass percent concentration 1%, obtains sarranine dye
Color slice；
(2) successively with the ethanol water of mass percent concentration 30%, 45%, 60% and 70% to sarranine stained slice
Gradient elution is carried out, de- stained slice is obtained；
(3) de- stained slice is cleaned with pickling solution, obtains pickling slice；
The pickling solution is the ethanol solution containing 13~15% glacial acetic acid of mass percent concentration；
(4) 1~3min of dyeing, quality percentage are sliced to pickling with the A Li Xinlan dyeing liquor of mass percent concentration 1%
The ethanol water of specific concentration 65~80% elutes, mounting.
Preferably, in the step (1), the solvent of sarranine dyeing liquor is water.
Preferably, in the step (2), the ethanol water of mass percent concentration 30%, 45%, 60% and 70%
Elution time is independently 1~3min.
Preferably, in the step (3), the solvent of the pickling solution is the ethanol water of mass percent concentration 65~75%
Solution.
Preferably, in the step (3), the time of cleaning is 0.5~2min.
Preferably, in the step (4), the solvent of A Li Xinlan dyeing liquor is the second of mass percent concentration 65~75%
Alcohol solution.
Preferably, in the step (4), mounting is carried out with glycerol.
Preferably, stained slice, microscopy will be obtained after step (4) described mounting, wherein red is that sclerenchyma contaminates, brilliant blue
Color is parenchyma cell dye.
Compared with prior art, beneficial effects of the present invention:
It is dense with mass percent the present invention provides the colouring method of prothenchyma (of wood) and parenchyma cell in a kind of difference timber
The sarranine dyeing liquor of degree 1% dyes 45~75min to Wooden slice, obtains sarranine stained slice；It is successively dense with mass percent
The ethanol water of degree 30%, 45%, 60% and 70% carries out gradient elution to sarranine stained slice, obtains de- stained slice；
De- stained slice is cleaned with pickling solution, obtains pickling slice；The pickling solution is to contain 13~15% ice of mass percent concentration
The ethanol solution of acetic acid；1~3min of dyeing, quality are sliced to pickling with the A Li Xinlan dyeing liquor of mass percent concentration 1%
The ethanol water of percent concentration 65~80% elutes, mounting.
A Li Xinlan is carboxylic group coloring agent.In timber xylem, carboxyl is located at cellulose and hemicellulose mostly
In, it is considered that carboxyl is not present in lignin, thus A Li Xinlan is generally not used for wood staining.The present invention is using pickling
Mode adjusts the pH value in dyeing medium and changes coloring agent activity, as long as control it is proper as long as to reach two kinds of cellular responses different,
Chemically ingredient angle distinguishes sclerenchyma and parenchyma cell in timber.As shown in the embodiment of the present invention and attached drawing, use
The method of the invention dyes pinus sylvestris var. mongolica slice, and not only heavy wall tracheid (pinus sylvestris var. mongolica prothenchyma (of wood)) is uniformly dyed red
Color, ray parenchyma cell are also uniformly dyed sapphirine, including including that the resinosis cell in resin canal is also dyed to sapphirine.Energy
Enough forms for clearly observing ray parenchyma cell and cross trachield.Being clearly observed cross trachield inner wall has zigzag to add
Thickness, zigzag thickening are present in hard pine, such as masson pine, Chinese pine and black pine.And in the soft pine such as Korean pine, Huashan pine and lacebark pine
Cross trachield interior walls be smooth in class.Zigzag, which is thickeied, is of great significance to timber identification with identification.
For the tissue division research similar in the prothenchyma (of wood) and this structure of parenchyma cell, dyeing provided by the invention
Method is more simple and effective compared with the existing technology.
Detailed description of the invention
Fig. 1 is the dyeing flow figure of embodiment 1；
Fig. 2 is the dyeing effect figure of embodiment 1；Wherein, a figure is cross section, and b figure is tangential section, and c figure is radial longitudinal section；Figure
Middle T indicates tracheid；R indicates wood radiaftive rays；RC indicates resin canal；RT indicates cross trachield；RP indicates ray parenchyma cell；
Fig. 3 is the dyeing effect partial enlarged view of embodiment 1；Wherein, d figure is cross section, and e figure is tangential section, and f figure is diameter
Section.
Specific embodiment
The present invention provides the colouring methods of prothenchyma (of wood) and parenchyma cell in a kind of difference timber, comprising the following steps:
(1) 45~75min is dyed to Wooden slice with the sarranine dyeing liquor of mass percent concentration 1%, obtains sarranine dye
Color slice；
(2) successively with the ethanol water of mass percent concentration 30%, 45%, 60% and 70% to sarranine stained slice
Gradient elution is carried out, de- stained slice is obtained；
(3) de- stained slice is cleaned with pickling solution, obtains pickling slice；
The pickling solution is the ethanol solution containing 13~15% glacial acetic acid of mass percent concentration；
(4) 1~3min of dyeing, quality percentage are sliced to pickling with the A Li Xinlan dyeing liquor of mass percent concentration 1%
The ethanol water of specific concentration 65~80% elutes, mounting.
In the present invention, the sarranine dyeing liquor is used for the prothenchyma (of wood) of stained wood, shows in the slice after the completion of dyeing
For red.In the present invention, the solvent of the sarranine dyeing liquor is preferably water, and sarranine is mixed with water to obtain the final product.In the present invention,
The dyeing time of the sarranine dyeing liquor is preferably 60min.
After obtaining sarranine stained slice, the present invention is successively with the second of mass percent concentration 30%, 45%, 60% and 70%
Alcohol solution carries out gradient elution to sarranine stained slice, obtains de- stained slice.The present invention uses 30~70% ethanol water
It is to remove the sarranine dyeing liquor unless combining on sclerenchyma that solution, which carries out gradient elution,.In the present invention, the quality hundred
Divide the elution time of the ethanol water of specific concentration 30%, 45%, 60% and 70% independent preferably 1~3min, more preferably
For 2min.After obtaining de- stained slice, the present invention cleans de- stained slice with pickling solution, obtains pickling slice；The pickling solution
For the ethanol solution containing 13~15% glacial acetic acid of mass percent concentration.The present invention cleans de- stained slice using pickling solution
Purpose is the pH value in order to adjust cell to be dyed, and pH value is made to maintain 5.5~6.5 range, for activating A Lixin
The activity of blue dyestuff, make its it is stable be attached on the parenchyma cell of timber, realize that clarity is high, dyeing of high resolution, into
And realize the differentiation to timber parenchyma cell and prothenchyma (of wood).
In the present invention, the solvent of the pickling solution is preferably the ethanol water of mass percent concentration 65~75%,
The more preferably ethanol water of mass percent concentration 70%.In the present invention, the time of the pickling is 0.5~2min,
More preferably 1min.
After obtaining pickling slice, the present invention will be sliced pickling with the A Li Xinlan dyeing liquor of mass percent concentration 1%
Dye 1~3min, the ethanol water elution of mass percent concentration 65~80%, mounting.
In the present invention, the parenchyma cell that A Li Xinlan dyeing liquor is used for as Wooden slice completes obtained dye in dyeing
In color slice, parenchyma cell is contaminated for sapphirine.In the present invention, the solvent of A Li Xinlan dyeing liquor is preferably quality hundred
Divide the ethanol water of specific concentration 65~75%, the more preferably ethanol water of mass percent concentration 70%.In the present invention
In, the dyeing time of A Li Xinlan dyeing liquor is preferably 2min.
After the present invention dyes A Li Xinlan dyeing liquor using the ethanol water of mass percent concentration 65~80%
Slice is eluted, and the Alcian blue dyes being attached on non-parenchyma cell are washed away；More preferably mass percent concentration 70%
Ethanol water.
In the present invention, the mounting preferably uses glycerol to carry out mounting, can also be known in the art using other
Mountant carries out mounting.
Obtain stained slice after colouring method mounting of the present invention, microscopy, it is red that prothenchyma (of wood), which is contaminated, in stained slice
Color, parenchyma cell are contaminated for sapphirine.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
(1) test material
A, about 22 years pinus sylvestris var. mongolica timber of the age of tree is taken, 10 centimetres of thick disks are taken at breastheight, laboratory gas is taken back and does, then make
15~20 μm of slices are cut with the formula slice rice that slips, stress release (pressing from both sides smooth, 70 DEG C of heating water bath 4h with plectrum) is spare afterwards；
B, medical fluid is configured, sarranine dyeing liquor is the sarranine aqueous solution of mass percent concentration 1%；A Li Xinlan coloring agent
In, solvent is 70% ethanol solution, and solute/(solvent+solute) is 1%；In pickling solution, solute is glacial acetic acid, solvent 70%
Ethanol solution, solute/(solvent+solute) are 13~15%.
(2) process is tested
Dyeing flow as shown in Figure 1, take the pinus sylvestris var. mongolica cured be sliced, with sarranine dyeing liquor dye 60min, obtain sarranine dye
Color slice；2min is successively eluted respectively with the ethanol water of mass percent concentration 30%, 45%, 60%, 70%, obtains de- dye
Color slice；
With pickling solution to de- stained slice pickling 1min, pickling slice is obtained；Pickling is sliced with A Li Xinlan dyeing liquor
After dyeing 2min, loose colour is cleaned with 70% ethyl alcohol of mass percent concentration, with glycerol mounting, microscopically observation.
(3) test result
The chemical component of pinus sylvestris var. mongolica tracheid and parenchyma cell has differences.Tracheid degree of lignification is higher than parenchyma cell, pipe
Born of the same parents' cell wall contains a large amount of lignin and the higher cellulose of crystallinity, parenchyma cell cell wall inner cellulose and hemicellulose level
Higher than lignin.Regulate and control the pH in dyeing medium and change coloring agent activity, two kinds of cellular responses can be reached as long as control is proper
Different, chemically ingredient angle distinguishes heavy wall pipe parenchyma cell in timber.
Microscopic examination result is as shown in Figures 2 and 3, it can be seen that not only heavy wall tracheid is uniformly dyed red, ray parenchyma
Cell is also uniformly dyed sapphirine, is also dyed to sapphirine including the resinosis cell in resin canal.It can clearly observe
To the form of ray parenchyma cell and cross trachield.Being clearly observed cross trachield inner wall has zigzag thickening, and zigzag adds
Thickness is present in hard pine, such as masson pine, Chinese pine and black pine.And the ray tube in the soft pines such as Korean pine, Huashan pine and lacebark pine
Born of the same parents' interior walls be smooth.Zigzag, which is thickeied, is of great significance to timber identification with identification.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. the colouring method of prothenchyma (of wood) and parenchyma cell in a kind of difference timber, comprising the following steps:
(1) 45~75min is dyed to Wooden slice with the sarranine dyeing liquor of mass percent concentration 1%, obtains sarranine dyeing and cuts
Piece；
(2) successively sarranine stained slice is carried out with the ethanol water of mass percent concentration 30%, 45%, 60% and 70%
Gradient elution obtains de- stained slice；
(3) de- stained slice is cleaned with pickling solution, obtains pickling slice；
The pickling solution is the ethanol solution containing 13~15% glacial acetic acid of mass percent concentration；
(4) 1~3min of dyeing is sliced to pickling with the A Li Xinlan dyeing liquor of mass percent concentration 1%, mass percent is dense
The ethanol water elution of degree 65~80%, mounting.
2. colouring method according to claim 1, which is characterized in that in the step (1), the solvent of sarranine dyeing liquor is
Water.
3. colouring method according to claim 1, which is characterized in that in the step (2), mass percent concentration
30%, the elution time of 45%, 60% and 70% ethanol water is independently 1~3min.
4. colouring method according to claim 1, which is characterized in that in the step (3), the solvent of the pickling solution is
The ethanol water of mass percent concentration 65~75%.
5. colouring method according to claim 1 or 4, which is characterized in that in the step (3), the time of cleaning is 0.5
~2min.
6. colouring method according to claim 1, which is characterized in that in the step (4), A Li Xinlan dyeing liquor it is molten
Agent is the ethanol water of mass percent concentration 65~75%.
7. colouring method according to claim 1 or 6, which is characterized in that in the step (4), carry out mounting with glycerol.
8. colouring method according to claim 1, which is characterized in that cut the dyeing obtained after step (4) described mounting
Piece carries out microscopy, wherein red is that sclerenchyma contaminates, sapphirine is parenchyma cell dye.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110954384A (en) * | 2019-12-16 | 2020-04-03 | 贵州大学 | Preparation method of masson pine resin tract paraffin section |
| CN111855360A (en) * | 2020-07-27 | 2020-10-30 | 安徽农业大学 | Pear fruit stone cell specific histochemical localization method |
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| CN104861724A (en) * | 2014-02-26 | 2015-08-26 | 哈尔滨市工艺美术有限责任公司 | Preparation formula and production method for pale turquoise wheat straw dye |
| CN106078990A (en) * | 2016-08-12 | 2016-11-09 | 梅州市汇胜木制品有限公司 | The imitative precious colouring method of a kind of timber |
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