CN109134037A - A method of Nitrate is reduced using wormcast - Google Patents
A method of Nitrate is reduced using wormcast Download PDFInfo
- Publication number
- CN109134037A CN109134037A CN201810863691.6A CN201810863691A CN109134037A CN 109134037 A CN109134037 A CN 109134037A CN 201810863691 A CN201810863691 A CN 201810863691A CN 109134037 A CN109134037 A CN 109134037A
- Authority
- CN
- China
- Prior art keywords
- vermicompost
- leafy vegetables
- nitrate
- nitrate content
- bacillus megaterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910002651 NO3 Inorganic materials 0.000 title claims abstract description 27
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000013311 vegetables Nutrition 0.000 claims abstract description 16
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 14
- 239000000618 nitrogen fertilizer Substances 0.000 claims abstract description 10
- 239000002689 soil Substances 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 5
- 238000009331 sowing Methods 0.000 claims abstract description 4
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 8
- 244000221633 Brassica rapa subsp chinensis Species 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 4
- 241000208822 Lactuca Species 0.000 claims description 2
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 2
- 241001233061 earthworms Species 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 230000001603 reducing effect Effects 0.000 abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 125000001477 organic nitrogen group Chemical group 0.000 abstract description 4
- 238000012364 cultivation method Methods 0.000 abstract 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 13
- 239000003337 fertilizer Substances 0.000 description 9
- 235000000318 Bindesalat Nutrition 0.000 description 6
- 244000106835 Bindesalat Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 241000361919 Metaphire sieboldi Species 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 239000004202 carbamide Substances 0.000 description 4
- 239000010410 layer Substances 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 150000002823 nitrates Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 108010025915 Nitrite Reductases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
本发明公开了一种利用蚯蚓粪降低叶菜硝酸盐含量的方法，具体为：在叶菜播种前，将蚯蚓粪、巨大芽孢杆菌和氮肥按照一定比例混合，并均匀撒施在土壤表层，再用旋耕机耙匀，使之与土壤充分混合。叶菜生长期间其他管理与常规管理模式相同。本发明栽培方法一方面利用蚯蚓粪提供的有机态氮替代部分无机态氮，另一方面通过添加巨大芽孢杆菌协同降低硝酸盐，从而达到降低叶菜硝酸盐含量、改善叶菜品质的目的。该发明适用于叶菜，可直接在叶菜生产中推广应用。The invention discloses a method for reducing nitrate content of leafy vegetables by utilizing vermicompost, specifically: before sowing the leafy vegetables, mixing vermicompost, Bacillus megaterium and nitrogen fertilizer according to a certain proportion, uniformly spreading them on the soil surface, and then Rake with a rotary tiller to mix well with the soil. Other management during the growth of leafy vegetables is the same as the conventional management mode. The cultivation method of the present invention utilizes organic nitrogen provided by vermicompost to replace part of inorganic nitrogen on the one hand, and synergistically reduces nitrate by adding Bacillus megaterium, thereby achieving the purpose of reducing the nitrate content of leafy vegetables and improving the quality of leafy vegetables. The invention is suitable for leafy vegetables and can be directly popularized and applied in the production of leafy vegetables.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of side that Nitrate is reduced using wormcast
Method.
Background technique
Leaf vegetables is victual indispensable in people's daily life, easily enrichment nitrate.Nitrate itself is nontoxic, but
During long-time storage, under enzyme and microbial action, it is easily reduced into nitrite, and nitrite is absorbed by the body
Afterwards, it can be reacted with protein breakdown products secondary amine under gastric acid effect and generate carcinogenicity substance --- nitrosamine, and then threatened
Human health.There is 72-94% from vegetables in the nitrate of human body intake, and leaf vegetables occupies China's vegetables overall supplies
24.6%.Therefore, nitrate content in leaf vegetables is reduced, is the important channel for guaranteeing food safety and human health.
Currently, the major measure for reducing nitrates accumulation includes: to reduce nitrogen fertilizer amount, selection Fertilizers and form, match
Close applied nitrogen inhibitor etc..Wherein compounding application nitrogenous fertilizer inhibitor is although nitrate reducing effect is preferable, by additive amount,
Addition time and soil environment are affected, and can gradually decrease as Crop growing stage extends its effect.Therefore it reduces nitrogenous fertilizer and uses
Organic nitrogenous fertilizer is measured and applied, is the important channel for reducing leaf vegetables nitrates accumulation.Wormcast is a kind of good organic fertilizer,
Rich in nutriments such as organic nitrogen, humus, nutrient is abundant and comprehensive.Bacillus megaterium is then by nitrate nitrogen with microorganism nitrogen
Form be fixed up, make nitrogen slow release, while generating nitrite reductase, weaken nitrogen deoxidation and oxidation process, from
And reduce nitrate content.One aspect of the present invention addition can provide the wormcast of organic nitrogen and weaken the huge of nitrogen oxidation process
Bacterium anthracoides, on the other hand reduce amount of application of nitrogen fertilizer, and the two cooperation reduces crop nitrate content.
Summary of the invention
In view of the problems of the existing technology, Nitrate is reduced using wormcast the present invention provides a kind of
Method.
The present invention is realized especially by following technical scheme:
A method of Nitrate is reduced using wormcast, comprising the following steps:
Before leaf vegetables sowing, according to wormcast 60-120t/hm2, bacillus megaterium 5-7kg/hm2, nitrogenous fertilizer 65-90kg/
hm2Amount of application, it is mixed, and uniformly spreads fertilizer over the fields in upper soll layer, then with rotary cultivator harrow it is even, be allowed to be sufficiently mixed with soil.
Other management of leaf vegetables growth period of the present invention are identical as Routine Management mode.
Further, the wormcast is that earthworm eats granule substance caused by cow dung.
Further, the wormcast is Zi production in use, no more than 3 moon standing time period.
Further, the bacillus megaterium living bacteria count is greater than or equal to 5 × 109/g。
Further, the leaf vegetables is any in romaine lettuce or pakchoi.
The invention has the benefit that
1) wormcast used in the present invention is rich in organic nitrogen, and the nitrogen that crop can be made to absorb more assimilates into amino acid
With soluble protein, thus nitrates accumulation caused by reducing because of excessive application inorganic states nitrogenous fertilizer.
2) wormcast used in the present invention is the granule substance eating cow dung using earthworm and generating, than common organic fertilizer
It is more clean, sanitary and safe, while also solving the problems, such as that discarded cow dung resourceization utilizes indirectly.
3) bacillus megaterium used in the present invention can not only reduce Nitrate, and can also will be in soil
Fixed phosphorus K released comes out, and promotes leaf vegetables growth, improves leaf vegetables quality.
Detailed description of the invention
Fig. 1 is influence of the different wormcast amounts of application to Nitrate Content in Lettuce；
Fig. 2 is influence of the different wormcast amounts of application to romaine lettuce yield；
Fig. 3 is influence of the different wormcast amounts of application to Nitrate Content in Pakchoi；
Fig. 4 is influence of the different wormcast amounts of application to yield of pakchoi.
Specific embodiment
Below in conjunction with specific embodiment of the present invention, technical solution of the present invention is clearly and completely described, is shown
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
The wormcast 11.99kg that standing time is less than 3 months is weighed, living bacteria count is greater than 5 × 109The huge bud of/g
Spore bacillus 1.00g, urea 38.73g are uniformly mixed, are spread fertilizer over the fields in 2m2On upper soll layer, then it is even with rotary cultivator rake.
Embodiment 2
The wormcast 17.99kg that standing time is less than 3 months is weighed, living bacteria count is greater than 5 × 109The huge bud of/g
Spore bacillus 1.20g, urea 33.66g are uniformly mixed, are spread fertilizer over the fields in 2m2On upper soll layer, then it is even with rotary cultivator rake.
Embodiment 3
The wormcast 23.98kg that standing time is less than 3 months is weighed, living bacteria count is greater than 5 × 109The huge bud of/g
Spore bacillus 1.40g, urea 28.59g are uniformly mixed, are spread fertilizer over the fields in 2m2On upper soll layer, then it is even with rotary cultivator rake.
Technical effect of the invention is illustrated below with reference to specific experimental program:
1, for trying leaf vegetables kind: romaine lettuce, pakchoi
2, experimental design
Processing 1: fertilizer treatment is according to embodiment 1
Processing 2: fertilizer treatment is according to embodiment 2
Processing 3: fertilizer treatment is according to embodiment 3
CK: urea 48.89g
It tests and is sowed on May 11st, 2018, June 16, harvest, lasted 35 days.Experimental design 4 processing, 3 repetitions,
Plot area is 2m × 1m=2m2.Fertilizer used disposable surface layer that is manured into soil before leaf vegetables sowing is tested, and uses rotary cultivator
It harrows even.Other management of leaf vegetables growth period are identical as Routine Management mode.When harvesting, Nitrate and yield are measured,
Correlated results is shown in Fig. 1-4.
By Fig. 1, Fig. 3 it is found that application wormcast and romaine lettuce can be effectively reduced in bacillus megaterium, pakchoi nitrate contains
Amount, wherein it is best to handle 2 reducing effects, compared with the control, can reduce respectively romaine lettuce, Nitrate Content in Pakchoi 38.98%,
45.86%.Wormcast and bacillus megaterium also have apparent effect to leaf vegetables yield is promoted.By Fig. 2, Fig. 4 it is found that application earthworm
The processing of earthworm excrement and bacillus megaterium, cell production are above control, and best with the effect of increasing production for handling 2.It follows that
Application wormcast and bacillus megaterium can effectively reduce romaine lettuce, Nitrate Content in Pakchoi, increase its yield, wherein wormcast
Amount of application is 90t/hm2, bacillus megaterium amount of application is 6kg/hm2Shi Xiaoguo is best.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understand without departing from the principles and spirit of the present invention can to these examples carry out it is a variety of variation, modification, replacement and
Modification, the scope of the present invention is defined by the appended.
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810863691.6A CN109134037A (en) | 2018-08-01 | 2018-08-01 | A method of Nitrate is reduced using wormcast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810863691.6A CN109134037A (en) | 2018-08-01 | 2018-08-01 | A method of Nitrate is reduced using wormcast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109134037A true CN109134037A (en) | 2019-01-04 |
Family
ID=64799475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810863691.6A Pending CN109134037A (en) | 2018-08-01 | 2018-08-01 | A method of Nitrate is reduced using wormcast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109134037A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948321A (en) * | 2012-11-01 | 2013-03-06 | 南京农业大学 | Method for reducing nitrate content in vegetable |
| CN103214306A (en) * | 2013-04-03 | 2013-07-24 | 广东省昆虫研究所 | Wormcast biological medicine fertilizer and preparation method thereof |
| CN104311347A (en) * | 2014-10-22 | 2015-01-28 | 山东宝源生物有限公司 | Microbial fertilizer as well as preparation method and application thereof |
| CN106386438A (en) * | 2016-08-31 | 2017-02-15 | 马山盛世生态种养专业合作社 | Spinach planting method capable of decreasing nitrate content |
-
2018
- 2018-08-01 CN CN201810863691.6A patent/CN109134037A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948321A (en) * | 2012-11-01 | 2013-03-06 | 南京农业大学 | Method for reducing nitrate content in vegetable |
| CN103214306A (en) * | 2013-04-03 | 2013-07-24 | 广东省昆虫研究所 | Wormcast biological medicine fertilizer and preparation method thereof |
| CN104311347A (en) * | 2014-10-22 | 2015-01-28 | 山东宝源生物有限公司 | Microbial fertilizer as well as preparation method and application thereof |
| CN106386438A (en) * | 2016-08-31 | 2017-02-15 | 马山盛世生态种养专业合作社 | Spinach planting method capable of decreasing nitrate content |
Non-Patent Citations (2)
| Title |
|---|
| 吴志行 等: "《蔬菜无公害生产技术》", 31 March 2009, 中原农民出版社 * |
| 贾秀英: "《环境科学研究进展》", 31 May 2008, 浙江大学出版社 * |
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| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |