CN109123189A - A kind of secondary solid fermented black tea slag and its preparation method and application - Google Patents
A kind of secondary solid fermented black tea slag and its preparation method and application Download PDFInfo
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- CN109123189A CN109123189A CN201811026336.XA CN201811026336A CN109123189A CN 109123189 A CN109123189 A CN 109123189A CN 201811026336 A CN201811026336 A CN 201811026336A CN 109123189 A CN109123189 A CN 109123189A
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Abstract
The invention discloses a kind of preparation method of secondary solid fermented black tea slag, include the following steps: fermenting microbe is added, ferments in 1000ml triangular flask after 75% black tea slag and 15% rapeseed meal after high pressure sterilization, the mixing of 20% cotton dregs；Wherein, 75% black tea slag, 15% rapeseed meal, 20% cotton dregs are mass percent.The invention also discloses a kind of secondary solid fermented black tea slag, a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old and feed.The present invention has the advantage that a kind of pH in digestive tract of the meat wild pheasant of 1-2 week old is effectively reduced in a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed compared with prior art, inhibit harmful microbe growth, promote beneficial microorganism breeding, optimize gut flora, improve nutritive digestibility, enhance the meat wild pheasant anti-heat stress ability of 1-2 week old, enhances its immunity and disease resistance, improve growth performance and survival rate.
Description
The present invention be application No. is: 201510579680.1, a kind of applying date are as follows: 2015-09-11, patent name are as follows: 1-
The divisional application of the meat wild pheasant premix material of anti heat stress of 2 week old and feed
Technical field
The present invention relates to a kind of meat wild pheasant feed more particularly to a kind of secondary solid fermented black tea slag and its systems
Preparation Method and application.
Background technique
It stress a kind of (stress) nonspecific response that be livestock and poultry generate poor environment or the stimulation of the internal body factor
Reaction, causes body nerve and endocrine anomalous variation, leads to metabolic, immune and antioxidant system disorder, to drop
Low breeding performonce fo animals, will lead to animal dead when serious.During modernization, intensive and scale animal and poultry cultivation, poultry
Fowl faces increasingly complex changeable stressor, such as environmental factor (drinking-water, dust, temperature, radiation, pernicious gas), disease because
Plain (harmful microorganism, diarrhea, inflammation etc.), feeding management factor (stocking density, transport, turn group, controlled feeding etc.).In short,
All cause body uncomfortable and the factor of body injury are caused to can be described as stressor.These stress factors constantly influence body mind
Through and Hormone system, generate a variety of bad physiological stresses, reduce its immunity and oxidation resistance, the element that has additional nutrients disappears
Consumption, finally declines livestock and poultry production performance, causes larger economic loss.
As consumer is higher and higher to meat quality requirement, wild pheasant meat at present obviously increases in China's number of animals raised
Add, cultivation situation is excellent, economic benefits.But meat wild pheasant nutrition and feed are studied in China also in first step
Section, is especially a lack of the feed formula of the meat wild pheasant of 1-2 week old, so that open country pheasant growth performance early period reduction of brooding, dead
Rate is higher, undoubtedly will cause biggish economic loss.
1-2 week old open country pheasant is very quick to stressor since the development such as physiological stage especially gastrointestinal function is unsound
Sense, is easy to happen stress reaction, to reduce production performance, improves the death rate, causes biggish economic loss.And 1-2 week old
Wild pheasant is very sensitive to various stressors, and especially nutritional ingredient needed for body is gradually switched to by yolk offer by nutriment
It provides, will cause enteron aisle stress.In addition, environmental change, transport, turn group etc. can wild pheasant young to meat cause different degrees of answer
Swash, reduces growth performance and survival rate.Therefore according to feeding standard, it would be highly desirable to develop that a kind of effect is obvious, effect is stablized, method letter
Just, a kind of meat wild pheasant premix material of anti heat stress of safe and non-toxic, noresidue 1-2 week old and feed.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of secondary solid fermented black tea slag and its systems
Preparation Method and application play resisting stress effect in terms of intestinal health, oxidative stress, neurological levels three, improve 1-2 week old meat
With wild mountain anti-heat stress ability.
The present invention is achieved by the following technical solutions: a kind of preparation method of secondary solid fermented black tea slag, including
Following steps:
(1) after with 75% black tea slag and 15% rapeseed meal after high pressure sterilization, the mixing of 20% cotton dregs, zymophyte is added
Kind, it ferments in 1000ml triangular flask；Wherein, 75% black tea slag, 15% rapeseed meal, 20% cotton dregs are mass percent；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis
With short and small spore bacillus, and bacillus subtilis (bacterial concentration) and short and small spore bacillus (bacterial concentration) are mixed with volume ratio 1:2 ratio
It closes, with the ratio mixed fermentation of 100-120g:1-1.05ml, the mixture that step (1) obtains is being 32 for fermentation material and Mixed Microbes
DEG C culture 3 days, one time fermentation is carried out, it is primary every 1h overturning in one time fermentation incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus, mixing secondary fermentation: are taken out
Fungi includes aspergillus oryzae and aspergillus niger, and aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, every 100g fermentation material inoculation
Mixed fungus.It is cultivated 3 days at 30 DEG C, carries out secondary fermentation, it is primary every 5h overturning in secondary fermentation incubation；
(4) it dries: after fermentation through step (3), the fermentation of the fermentation material that baking step (3) obtains, as secondary solid
Black tea slag.
The invention also discloses a kind of secondary solid fermented black tea slag, the secondary solid fermented black tea slag is wanted by right
A kind of preparation method of secondary solid fermented black tea slag described in asking 1 is prepared.
The invention also discloses a kind of applications of secondary solid fermented black tea slag, it is characterised in that: the secondary solid hair
Ferment black tea slag is used to prepare the pre- feed of the meat wild pheasant anti-heat stress of 1-2 week old.
The invention also discloses a kind of application of secondary fermentation black tea slag, the secondary solid fermented black tea slag is used to prepare
The meat wild pheasant premix material of anti heat stress of 1-2 week old.
The invention also discloses a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old, which is characterized in that with parts by weight
Meter, it is composed of the following components: fumaric acid 5, citric acid 10, sodium Diacetate 2, sodium acetate 2, sodium butyrate 4, phenylacetic acid 1, benzoic acid
6, glutamine 100, yeast selenium 30, astaxanthin 3, γ-aminobutyric acid 12, composite oligosaccharide 130, secondary solid fermented black tea slag
695, the secondary solid fermented black tea slag passes through a kind of preparation method of secondary solid fermented black tea slag described in claim 1
It is prepared.
As advanced optimizing for above scheme,
In parts by weight, composed of the following components: fumaric acid 10, citric acid 8, sodium Diacetate 3, sodium acetate 2, sodium butyrate
4, phenylacetic acid 1, benzoic acid 6, glutamine 90, yeast selenium 30, astaxanthin 3, γ-aminobutyric acid 10, composite oligosaccharide 120, two
Secondary solid fermentation black tea slag 713.
As advanced optimizing for above scheme,
The composite oligosaccharide is that mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soybean are low
Two kinds in glycan, xylo-oligosaccharide and its more than the oligosaccharide that is mixed with arbitrary proportion.
The invention also discloses a kind of meat wild pheasant anti-heat stress feeds of 1-2 week old, in parts by weight, by following components
Composition: corn 40-50, dregs of beans 15-20, rapeseed meal 5-10, fish meal 1-2, bone meal 1-2, wheat 15-20, earthworm protein powder 0-1, wheat
Bran 3-5；Soya-bean oil 0-1.5, rape oil 0-0.5, palm oil 0-0.5, calcium carbonate 1-1.8, calcium monohydrogen phosphate 1-1.5, DL- METHIONINE 0-2,
Additive premix 2-2.5 and premix material of anti heat stress 1-2, the premix material of anti heat stress are claim 5-7 any described
A kind of meat wild pheasant premix material of anti heat stress of 1-2 week old.
As advanced optimizing for above scheme,
In parts by weight, composed of the following components: corn 42.1, dregs of beans 16, rapeseed meal 7, fish meal 2, bone meal 1-2, wheat 20,
Earthworm protein powder 1, wheat bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil 0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix
Material 2.5 and premix material of anti heat stress 2.
As advanced optimizing for above scheme,
In parts by weight, composed of the following components: corn 41.5, dregs of beans 15.6, rapeseed meal 8, fish meal 2, bone meal 1, wheat 20,
Earthworm protein powder 1, wheat bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil 0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix
Material 2.5 and premix material of anti heat stress 1.6.
Compared with the prior art, a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed has
Beneficial effect is embodied in:
It 1, include fumaric acid, lemon in a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed
Lemon acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid and benzoic acid, relative to the single acidulant in existing formula, effect
It is good, a kind of pH in digestive tract of the meat wild pheasant of 1-2 week old is effectively reduced, inhibits harmful microbe growth, promotes beneficial to micro- life
The breeding of object optimizes gut flora, improves nutritive digestibility, the meat wild pheasant anti-heat stress ability of enhancing 1-2 week old.
It wherein, also include glutamy in a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed
Amine, yeast selenium, shrimp clear element, composite oligosaccharide, γ-aminobutyric acid, secondary solid fermented black tea slag, the above each element is in the present invention
A kind of meat wild pheasant premix material of anti heat stress of 1-2 week old and feed in effect it is as follows:
Glutamine: improving the meat wild pheasant anti-stress effect of 1-2 week old and immunity, promotes the meat wild mountain of 1-2 week old
The growth and development of chicken small intestine and intestinal villus morphosis are normal, promote the anabolism of a variety of nutrients, alleviate more caused by heat stress
Kind stress damage.
Yeast selenium: being high-quality organic selenium source, and absorptivity is high, has significant anti-stress effect, and it is meat to enhance 1-2 week old
The immunity of wild pheasant.
Astaxanthin i.e. 3,3 '-dihydroxy -4,4 '-diketo-β, β '-carrotene, be the strongest antioxidant of nature it
One, can effective removing machine interior free yl, improve the anti-stress ability of the meat wild pheasant of 1-2 week old, raising stress when 1-2 week old
The growth performance of meat open country pheasant.
Composite oligosaccharide is also known as chemical probiotic, can improve micro-ecological environment, be conducive to beneficial bacterium such as bacillus licheniformis,
The proliferation of bacillus subtilis etc., metabolite organic acid reduce intestinal pH, inhibit harmful bacteria such as salmonella, Escherichia coli
With the breeding of spoilage organisms, intestinal health is safeguarded, improve the meat wild pheasant anti-stress ability of 1-2 week old.
Secondary solid fermented black tea slag, cost is relatively low, containing a certain amount of tea polyphenols, including flavanol compound (catechin),
Anthocyanin class, flavonoids, flavonols and phenolic acid class etc. can remove the meat wild pheasant interior free yl of 1-2 week old, have relatively strong
Removing toxic substances and the effect of anti-oxidation stress.In addition, secondary solid fermented black tea slag has special aroma, 1-2 week old meat can be improved
With wild pheasant feed intake, promote nutrient absorption.
A kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and the compound resisting stress additive of feed conversion
Improve dorking heat-resistant stress ability, fumaric acid in additive, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid,
Benzoic acid, glutamine and oligosaccharide can improve livestock raised for meat open country pheasant anti-stress ability in terms of safeguarding intestinal health；Yeast selenium,
Astaxanthin, secondary solid fermented black tea slag (effective component: tea polyphenols etc.) can remove livestock raised for meat open country pheasant machine interior free yl, improve
Anti-oxidation stress ability；γ-aminobutyric acid can play anti-stress ability from neurological levels.A kind of 1-2 week old of the invention is meat
Wild pheasant premix material of anti heat stress and feed play resisting stress function in terms of intestinal health, oxidative stress, neurological levels three
Effect has preferable application effect.
2, a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed, optimize 1-2 week old open country pheasant
Intestinal microflora promotes the growth of beneficial bacterium, safeguards intestinal health, and the meat wild pheasant of 1-2 week old is made to adapt to feed as early as possible
Behavior.
3, a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed, improve the meat open country of 1-2 week old
The anti-oxidation stress ability of pheasant, enhances its immunity and disease resistance, improve the meat wild pheasant of 1-2 week old growth performance and
Survival rate；
4, a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed, the feedstuff used, paddy
Glutamine, sodium Diacetate, black tea slag, mixed bacteria etc. meet forage health standard, the Ministry of Agriculture and national standard, will not be to meat
The nutritive value of chicken causes adverse effect, more safety and environmental protection.
Specific embodiment
It elaborates below to the embodiment of the present invention, the embodiment of the present invention carries out under premised on the technical program
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of meat wild pheasant premix material of anti heat stress of 1-2 week old, it is in parts by weight, composed of the following components: rhizoma corydalis
Acid 5, citric acid 10, sodium Diacetate 2, sodium acetate 2, sodium butyrate 4, phenylacetic acid 1, benzoic acid 6, glutamine 100, yeast selenium 30,
Astaxanthin 3, γ-aminobutyric acid 12, composite oligosaccharide 130, secondary solid fermented black tea slag 695.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 1
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 1 is the mixing oligosaccharide of mannan-oligosaccharides and oligomeric maltose, and sweet dew is oligomeric
Sugar and oligomeric maltose are with the mixing of 1:1 ratio.
Secondary solid fermented black tea slag in embodiment 1 is prepared by following processing technologys:
(1) after with 75% black tea slag and 15% rapeseed meal after high pressure sterilization, the mixing of 20% cotton dregs, zymophyte is added
Kind, it ferments in 1000ml triangular flask；Wherein, 75% black tea slag, 15% rapeseed meal, 20% cotton dregs are mass percent.
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis
With short and small spore bacillus, and bacillus subtilis (bacterial concentration OD600=0.5-1) and short and small spore bacillus (bacterial concentration OD600=
0.5-1) with the mixing of volume ratio 1:2 ratio, fermentation material and Mixed Microbes with the ratio mixed fermentation of 100-120g:1-1.05ml, step
Suddenly the mixture that (1) obtains is cultivated 3 days for 32 DEG C, carries out one time fermentation, is overturn in one time fermentation incubation every 1h
Once；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus, mixing secondary fermentation: are taken out
Fungi includes aspergillus oryzae and aspergillus niger, and aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, every 100g fermentation material inoculation 1
×106-1×108A mixed fungus.It is cultivated 3 days at 30 DEG C, carries out secondary fermentation, turned in secondary fermentation incubation every 5h
Turn primary；
(4) it dries: after fermentation through step (3), the fermentation of the fermentation material that baking step (3) obtains, as secondary solid
Black tea slag.
Embodiment 2
A kind of meat wild pheasant premix material of anti heat stress of 1-2 week old, in parts by weight, composed of the following components:
Fumaric acid 10, citric acid 8, sodium Diacetate 3, sodium acetate 2, sodium butyrate 4, phenylacetic acid 1, benzoic acid 6, glutamine
90, yeast selenium 30, astaxanthin 3, γ-aminobutyric acid 10, composite oligosaccharide 120, secondary solid fermented black tea slag 713.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 2
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 2 is isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide
Mixing oligosaccharide, wherein isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide are mixed with the ratio of 1:2:1:2
It closes.
Secondary solid fermented black tea slag in embodiment 2 is prepared by following processing technologys:
(1) after with 80% black tea slag and 13% rapeseed meal after high pressure sterilization, the mixing of 7% cotton dregs, zymophyte is added
Kind, it ferments in 1000ml triangular flask；Wherein, 80% black tea slag, 13% rapeseed meal, 7% cotton dregs are mass percent.
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis
With short and small spore bacillus, and bacillus subtilis (bacterial concentration OD600=0.5-1) and short and small spore bacillus (bacterial concentration OD600=
0.5-1) with the mixing of volume ratio 1:1 ratio, fermentation material and Mixed Microbes with the ratio mixed fermentation of 100-120g:1-1.05ml, step
Suddenly the mixture that (1) obtains is cultivated 2.5 days for 32 DEG C, is carried out one time fermentation, is turned in one time fermentation incubation every 1h
Turn primary；
(3) secondary fermentation: after one time fermentation, the fermentation material of step (2) is taken out, renewed vaccination mixed fungus is described
Mixed fungus includes aspergillus oryzae and aspergillus niger, wherein aspergillus oryzae and aspergillus niger are with the mixing of 1:2 ratio, every 100g fermentation material inoculation 1
×106-1×108A mixed fungus.It is cultivated 4 days at 28 DEG C, carries out secondary fermentation, turned in secondary fermentation incubation every 4h
Turn primary；
(4) it dries: after fermentation through step (3), the fermentation of the fermentation material that baking step (3) obtains, as secondary solid
Black tea slag.
Embodiment 3
A kind of meat wild pheasant anti-heat stress feed of 1-2 week old, in parts by weight, composed of the following components: corn 42.1,
Dregs of beans 16, rapeseed meal 7, fish meal 2, bone meal 1-2, wheat 20, earthworm protein powder 1, wheat bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil
0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix 2.5 and premix material of anti heat stress 2.
Wherein additive premix includes vitamin, microelement and enzyme preparation.Vitamin is selected from vitamin A, vitamin
E, one of vitamine D3, vitamin B1, vitamin B2, vitamin, vitamin, niacin, pantothenic acid, folic acid and biotin, two
Kind is a variety of；Microelement is in copper sulphate, ferrous sulfate, manganese sulfate, zinc sulfate, sodium selenite, calcium iodate and cobalt chloride
One kind, two or more.
Embodiment 4
A kind of meat wild pheasant anti-heat stress feed of 1-2 week old, in parts by weight, composed of the following components: corn 41.5,
Dregs of beans 15.6, rapeseed meal 8, fish meal 2, bone meal 1, wheat 20, earthworm protein powder 1, wheat bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil
0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix 2.5 and premix material of anti heat stress 1.6.
Experiment one:
It is compared using single factor test, utilizes a kind of meat wild pheasant anti-heat stress feed of 1-2 week old and routine of the invention
Meat wild pheasant forage feed 1 day meat wild pheasant of 1-2 week old, every group 180, tests 2 weeks, free water and feeding, experiment
Feed intake, weight and survival rate are recorded in the process, referring specifically to table 1.
Table 1
Wherein: a, b-difference represent significant difference (P < 0.05) .n=180 broiler chicken；SEM-standard error
Experimental group and control group initial weight are without significant difference, after cycling hot stress be handled, addition resisting stress premix
End weight, weight gain, feed intake and the survival rate of test group are significantly higher than control group.Heat stress is substantially reduced meat wild pheasant productivity
Can, and a kind of meat wild pheasant anti-heat stress feed of 1-2 week old of the invention larger can alleviate this negative effect, have compared with
Good anti-heat stress effect.
Experiment two:
It is compared using single factor test, utilizes a kind of meat wild pheasant anti-heat stress feed of 1-2 week old and routine of the invention
Meat wild pheasant forage feed 1 day meat wild pheasant of 1-2 week old, every group 100, tests 120 days, free water and feeding are real
Record feed intake and weight during testing.Wherein, first 15 days, the 1-2 week old that experimental group feeds the preferred embodiment of the present invention 4 was meat
The meat wild pheasant premix material of anti heat stress of the 1-2 week old of wild pheasant anti-heat stress feed+embodiment 2, control group feed routine 1-2
The meat wild pheasant feed of week old.From -120 age in days of 15 age in days, experimental group and control group feed identical perfect compound feed.
The result of test two: when 14 age in days, test group average weight is 74.5g, and feedstuff-meat ratio is 1.73, the death rate
0.81%；Control group average weight is 70.5g, and feedstuff-meat ratio is 1.78, the death rate 1.92%.When 120 age in days, test group is averaged body
Weight is 1638g, and feedstuff-meat ratio is 3.22, the death rate 1.42%；Control group average weight is 1512g, and feedstuff-meat ratio is 3.43, the death rate
2.19%.
As can be seen from the results, it feeds a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old of the invention and feed is effective
The weight for improving meat wild pheasant, reduces its death rate and feedstuff-meat ratio, can create bigger economic benefit.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of preparation method of secondary solid fermented black tea slag, which comprises the steps of:
(1) after with 75% black tea slag and 15% rapeseed meal after high pressure sterilization, the mixing of 20% cotton dregs, fermenting microbe is added,
It ferments in 1000ml triangular flask；Wherein, 75% black tea slag, 15% rapeseed meal, 20% cotton dregs are mass percent；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis and short
Small spore bacillus, and bacillus subtilis (bacterial concentration OD600=0.5-1) and short and small spore bacillus (bacterial concentration OD600=0.5-1)
With the mixing of volume ratio 1:2 ratio, with the ratio mixed fermentation of 100-120g:1-1.05ml, step (1) is obtained for fermentation material and Mixed Microbes
The mixture arrived is cultivated 3 days for 32 DEG C, carries out one time fermentation, primary every 1h overturning in one time fermentation incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus, mixed fungus secondary fermentation: are taken out
Including aspergillus oryzae and aspergillus niger, and aspergillus oryzae and aspergillus niger be with the mixing of volume ratio 1:1 ratio, and every 100g fermentation material inoculation 1 ×
106-1×108A mixed fungus.It is cultivated 3 days at 30 DEG C, carries out secondary fermentation, overturn in secondary fermentation incubation every 5h
Once；
(4) it dries: after fermentation through step (3), the fermentation material that baking step (3) obtains, as secondary solid fermented black tea
Slag.
2. a kind of secondary solid fermented black tea slag, it is characterised in that: the secondary solid fermented black tea slag passes through claim 1 institute
A kind of preparation method for the secondary solid fermented black tea slag stated is prepared.
3. a kind of application of secondary solid fermented black tea slag, it is characterised in that: the secondary solid fermented black tea slag is used to prepare
The pre- feed of the meat wild pheasant anti-heat stress of 1-2 week old.
4. a kind of application of secondary fermentation black tea slag, it is characterised in that: the secondary solid fermented black tea slag is used to prepare 1-2 weeks
Age meat wild pheasant premix material of anti heat stress.
5. a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old, which is characterized in that in parts by weight, by following components group
At: fumaric acid 5, citric acid 10, sodium Diacetate 2, sodium acetate 2, sodium butyrate 4, phenylacetic acid 1, benzoic acid 6, glutamine 100,
Yeast selenium 30, astaxanthin 3, γ-aminobutyric acid 12, composite oligosaccharide 130, secondary solid fermented black tea slag 695, it is described secondary solid
Body fermented black tea slag is prepared by a kind of preparation method of secondary solid fermented black tea slag described in claim 1.
6. a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old according to claim 5, which is characterized in that with weight
Part meter is measured, it is composed of the following components: fumaric acid 10, citric acid 8, sodium Diacetate 3, sodium acetate 2, sodium butyrate 4, phenylacetic acid 1, benzene
Formic acid 6, glutamine 90, yeast selenium 30, astaxanthin 3, γ-aminobutyric acid 10, composite oligosaccharide 120, secondary solid fermentation are red
Tea grounds 713.
7. a kind of meat wild pheasant premix material of anti heat stress of 1-2 week old according to claim 5, it is characterised in that: described
Composite oligosaccharide is mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide
In two kinds and its more than with arbitrary proportion mix oligosaccharide.
8. a kind of meat wild pheasant anti-heat stress feed of 1-2 week old, which is characterized in that in parts by weight, composed of the following components:
Corn 40-50, dregs of beans 15-20, rapeseed meal 5-10, fish meal 1-2, bone meal 1-2, wheat 15-20, earthworm protein powder 0-1, wheat bran 3-5；
Soya-bean oil 0-1.5, rape oil 0-0.5, palm oil 0-0.5, calcium carbonate 1-1.8, calcium monohydrogen phosphate 1-1.5, DL- METHIONINE 0-2, additive
Premix 2-2.5 and premix material of anti heat stress 1-2, the premix material of anti heat stress are any described one kind of claim 5-7
The meat wild pheasant premix material of anti heat stress of 1-2 week old.
9. a kind of meat wild pheasant anti-heat stress feed of 1-2 week old according to claim 5, which is characterized in that with weight
Part meter, it is composed of the following components: corn 42.1, dregs of beans 16, rapeseed meal 7, fish meal 2, bone meal 1-2, wheat 20, earthworm protein powder 1, wheat
Bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil 0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix 2.5 and anti-heat stress
Premix 2.
10. a kind of meat wild pheasant anti-heat stress feed of 1-2 week old according to claim 5, which is characterized in that with weight
Part meter, it is composed of the following components: corn 41.5, dregs of beans 15.6, rapeseed meal 8, fish meal 2, bone meal 1, wheat 20, earthworm protein powder 1, wheat
Bran 3；Soya-bean oil 1.5, rape oil 0.5, palm oil 0.5, calcium carbonate 1.5, calcium monohydrogen phosphate 1.4, additive premix 2.5 and anti-heat stress
Premix 1.6.
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| CN105076863B (en) | 2018-10-19 |
| CN109170315A (en) | 2019-01-11 |
| CN105076863A (en) | 2015-11-25 |
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