CN109105236A - Garlic cultivar seedling germination method and its application - Google Patents
Garlic cultivar seedling germination method and its application Download PDFInfo
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- 240000002234 Allium sativum Species 0.000 title claims abstract description 121
- 235000004611 garlic Nutrition 0.000 title claims abstract description 119
- 238000000034 method Methods 0.000 title claims abstract description 43
- 230000035784 germination Effects 0.000 title claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 238000005286 illumination Methods 0.000 claims abstract description 14
- 238000009402 cross-breeding Methods 0.000 claims abstract description 6
- 230000008569 process Effects 0.000 claims abstract description 5
- 241001290610 Abildgaardia Species 0.000 claims description 33
- 239000007640 basal medium Substances 0.000 claims description 28
- 210000001672 ovary Anatomy 0.000 claims description 24
- 229930006000 Sucrose Natural products 0.000 claims description 19
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 19
- 239000005720 sucrose Substances 0.000 claims description 19
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 16
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 239000000443 aerosol Substances 0.000 claims description 13
- 230000010152 pollination Effects 0.000 claims description 12
- 238000009395 breeding Methods 0.000 claims description 11
- 239000004471 Glycine Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 238000012549 training Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 241000383638 Allium nigrum Species 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 2
- 108010079058 casein hydrolysate Proteins 0.000 claims 10
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 claims 4
- 235000009508 confectionery Nutrition 0.000 claims 4
- 238000004140 cleaning Methods 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 abstract description 13
- 230000001954 sterilising effect Effects 0.000 abstract description 7
- 230000014639 sexual reproduction Effects 0.000 abstract description 4
- 241000557833 Hua gabonii Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 238000002560 therapeutic procedure Methods 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000234282 Allium Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Inorganic materials [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- BZSXEZOLBIJVQK-UHFFFAOYSA-N 2-methylsulfonylbenzoic acid Chemical compound CS(=O)(=O)C1=CC=CC=C1C(O)=O BZSXEZOLBIJVQK-UHFFFAOYSA-N 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229910004619 Na2MoO4 Inorganic materials 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 230000011681 asexual reproduction Effects 0.000 description 1
- 238000013465 asexual reproduction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000020706 garlic extract Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 229940064880 inositol 100 mg Drugs 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 229910052603 melanterite Inorganic materials 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses garlic cultivar seedling germination method and its applications.For the technical solution for obtaining garlic seedling by garlic sexual seed is lacked in the prior art, the method for obtaining seedling is sprouted via the sexual seed of garlic cultivar the present invention provides a kind of.This method is seeded in No. 1 culture medium of seed after sterilizing the fresh seeds of garlic cultivar, and 15d~30d is cultivated under preference temperature, illumination condition；No. 2 culture mediums of seed are set in switching, and 15d~30d is cultivated under preference temperature, illumination condition；No. 3 culture mediums of seed are set in switching, and culture to seed sprouts seedling out under proper temperature temperature, illumination condition.The present invention also provides application of the garlic cultivar seedling germination method in garlic crossbreeding.Present approach provides the sexual seed of garlic cultivar to be seeded in the method sprouted on culture medium and obtain garlic seedling, prior art vacancy is filled up, has kept entire garlic sexual reproduction technique process complete.
Description
Technical field
The present invention relates to cultivation method of garlic, hybridize more particularly to garlic cultivar seedling breeding method with garlic
Breeding method belongs to agricultural technology, vegetable growing breeding technical field.
Background technique
Garlic (Allium sativum L.) is the second largest allium object that current cultivated area in the world is only second to onion
Kind.Common garlic cultivar cannot form seed since field cannot normally bloom under field conditions (factors), and then infertility.It is existing
There is garlic cultivation that can only carry out vegetative propagation by bulbil or aerosol index, cannot achieve sexual propagation.Sexual reproduction technique
Lack, limits and cultivated in garlic cultivation using the means of the sexual hybridization polymerization limited genetic diversity of Garlic Germplasm Resources
A possibility that improved Varieties.Thus, garlic breeding methods are mainly limited to nature or induced mutation at present and body cell is asexual
The asexual reproduction methods such as system's variation.Long-term vegetative propagation infects garlic susceptible viral, and easily causes kind of a sexual involution, leads to garlic
Yield and quality decline.
Application publication number is that the Chinese patent application of 103858754 A of CN discloses a kind of anti-season garlic new varieties
Mating system.This method rubs scattered threshing in nursery step, by the aerosol index in involucre, chooses the gas life for meeting certain index
Bulb is sowed as seed, is transplanted after obtaining seedling.In harvest step, involucre is cut, aerosol index is rubbed scattered into threshing, selected
For the seed first generation.These operations are repeated in later cultivation.This method although declare its realize garlic from vegetative propagation to
The dramatic shift of sexual propagation, but in fact, " seed " used in this method operation is to rub scattered threshing by aerosol index to obtain
, rather than solid acquisition is developed by ovule fertilization.Therefore, " seed " alleged by this method is not really to anticipate in plant developmental biology
" seed " concept of justice, the seedling turned out nor seedling.The mating system is substantially nutrition organs (the raw squama of gas by garlic
Stem) to be detached from after parent differentiation and development to be substantially again a kind of to utilize nutrition organs realization at a complete garlic plant
Nourish and generate, belong to vegetative propagation rather than sexual propagation.
The prior art wishes to solve the technical issues of obtaining garlic seedling by garlic sexual seed always, to be
Garlic cultivar advantage is persistently saved, and is laid the foundation to obtain more excellent garlic cultivar by crossbreeding.Garlic sexual kind
Son is developed in garlic ovary by the ovule after inseminating.The prior art, which has been resolved, implements people for garlic cultivar
Work is pollinated (CN1656883A), sexual embryo cultivates (CN1656885A, CN101396003A), the anti-pollution cultivation of ovary
(CN102257966A) the problems such as finally harvesting garlic seed.But the problem of obtaining seedling (emerging) from the sprouting of garlic seed
Not yet solve.It is existing research only obtain " generally speaking garlic true seed viability is weaker, some must on culture medium ability
The descriptive conclusion of germination " is (referring to Xu Peiwen etc., garlic germplasm innovation and Research Advances on Breeding, " China's Vegetable " 2006 (6)；
Guo Ruifeng etc., garlic rapeseed breeding technology Evolution, " Agriculture of Anhui science " 2011,39 (5)), lack real technical solution.
Summary of the invention
The purpose of the present invention is to the deficiencies in the prior art, provide one kind and sprout via the sexual seed of garlic cultivar
The method that hair obtains seedling.
To achieve the above object, the present invention provides a kind of garlic cultivar seedling germination method, and technical solution is such as
Under:
Garlic cultivar seedling germination method, it is characterised in that: after sterilizing the fresh seeds of garlic cultivar
It is seeded in No. 1 culture medium of seed, 15d~30d is cultivated under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d；Kind is set in switching
Sub No. 2 culture mediums, cultivate 15d~30d under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d；Seed 3 trainings are set in switching
Base is supported, culture to seed sprouts seedling out under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d；The garlic cultivation
Kind is bloom type garlic cultivar；No. 1 culture medium of the seed is the addition hydrolysis junket egg in every liter of B5 basal medium
White 100g~800g, glycine 0.5mg~3.0mg, sucrose 25g~60g, every liter agar solidified with 6g~7g, adjusts pH to 5.8
~6.0；No. 2 culture mediums of the seed are that 6-BA0.01mg~2.00mg, hydrolysis junket egg are added in every liter of B5 basal medium
White 100g~800g, glycine 0.5mg~3.0mg, sucrose 25g~60g, every liter agar solidified with 6g~7g, adjusts pH to 5.8
~6.0；No. 3 culture mediums of the seed be in every liter of B5 basal medium add 6-BA0.5mg~2.0mg, NAA0.01mg~
1.00mg, sucrose 25g~45g, every liter agar solidified with 6g~7g, adjusts pH to 5.8~6.0.
Above-mentioned garlic cultivar seedling breeding method acquires in the prior art and obtains garlic cultivar fresh seeds
On the basis of, by garlic seed plant in promote on culture medium its germination and growth until obtain seedling.3 stages of incubation point
Different nutrients is provided for seed sprouting activity, solves the problems, such as that garlic true seed viability is weaker with synthesis.Garlic kind
The acquisition of son is first to cultivate garlic cultivar to flower a kind of sedge phase, cuts colored a kind of sedge (with petal), rejects aerosol index, then
Flower a kind of sedge is cultivated to when garlic bud is open, pollen scatters, artificial pollination is implemented；Continue to cultivate, ovary full to ovary increasing
In fertilized ovule develop into black seeds；Clip contains the ovary of black garlic seed；Ovary is sterilized, by garlic seed from
It is separated in ovary.In the method for the present invention, seedling is obtained by then passing through cultivating seeds, thus used garlic cultivates product
Kind, which refers to, overcomes nature kind defect by artificially breeding, spends the garlic cultivar that a kind of sedge is good, can blossom and have seeds.This kind of cultivar
It is a kind of type garlic cultivar that blossoms and has seeds through artificially breeding.
In the above method, flower a kind of sedge phase garlic cuts flower a kind of sedge with petal from away from ground 5cm~25cm, thereafter until son
Big garlic flower a kind of sedge (having petal) culture of room maturation can be trained in all kinds of liquid to be completed.Garlic adaptability nutrient is imposed in nutrient solution.?
The aerosol index for removing remaining in time is needed in cultivating process, it is therefore an objective to reduce the competition with bud nutrient.When artificial pollination, with point
Head tweezers remove the anther of the pollen that scatters, on then column cap that the pollen on anther is awarded to different buds, if column cap cannot be stretched
It is long, break petal into two with one's hands pollination.
Garlic cultivar seedling germination method of the present invention is to develop into the fertilization of garlic ovule using artificial pollination
Seed culture Germination And Seedling belongs to complete sexual reproduction process on biological significance, thus can be used for garlic crossbreeding.
Therefore the application present invention simultaneously provides garlic cultivar seedling germination method in garlic crossbreeding.
Compared with prior art, the sexual seed of garlic cultivar is inoculated with the beneficial effects of the present invention are: providing
The method for obtaining garlic seedling is sprouted on culture medium, has been filled up prior art vacancy, has been made entire garlic sexual reproduction technique
Process is complete, lays a good foundation for garlic crossbreeding.
Specific embodiment
Below with reference to embodiment, the preferred technical solution of the present invention is further described.
Embodiment one
It is emerged with the method for the present invention culture garlic cultivar seedling.
1, material agents
1.1 garlic plant materials
Garlic cultivar G398, the long-term breed conservation material of academy of agricultural sciences of Shandong Province vegetable or flower institute；Garlic cultivar
09-3, vegetable or flower institute of academy of agricultural sciences of Shandong Province breed conservation material.
1.2 culture medium
Flower a kind of sedge phase nutrient solution: KH2PO450mg/L, KNO3500mg/L, (NH4)2SO450mg/L, MgSO25mg/L,
CaCl230mg/L, H3BO40.1mg/L, Sucrose 25g/L.
B5 basal medium: KNO32500mg/L, MgSO4·7H2O 250mg/L, CaCl2·2H2O 150mg/L,
(NH4)2SO4134mg/L, NaH2PO4.H2O 150mg/L, KI 0.75, H3BO33.0, MnSO4·4H2O, 10mg/L,
ZnSO4·7H2O 2.0mg/L, Na2MoO4·2H2O 0.25mg/L, CoCl2·6H2O 0.025mg/L, CuSO4·5H2,
0.025mg/L, Na2- EDTA 37.3mg/L, FeSO4·7H2O 27.8mg/L, inositol 100mg/L, niacin 1.0mg/L, hydrochloric acid
Pyridoxol (VB6) 1.0mg/L, thiamine hydrochloride (VB1)10.0mg/L。
Seed 1, No. 2, No. 3 culture mediums are the components shown in addition table 1 respectively into 1L B5 basal medium, and adjust
PH to 5.8~6.0, high pressure sterilization are spare.
1 embodiment of table, one seed 1, No. 2, addO-on therapy in No. 3 culture mediums
2, culture operation
2.1 cultivate garlics to flower a kind of sedge phase
Garlic cultivar G398,09-3 are sowed with bulbil, and culture to garlic extracts flower a kind of sedge out under the conditions of field cultivation.
2.2. big garlic flower a kind of sedge liquid training
2d~18d after big garlic flower a kind of sedge extraction, to observe that aerosol index and bud are grown to index simultaneously, respectively from away from
Flower a kind of sedge with petal is cut at the 5cm~25cm of ground, rejects aerosol index, and tap water rinses scape notch 20min, distilled water
It rinses, flower a kind of sedge notch is put into downward in the non-toxic plastic container for filling 1000ml~2000ml flower a kind of sedge phase nutrient solution and carries out liquid
Training.Plastic containers are put into worker's incubator, and condition of culture is 25 ± 2 DEG C of temperature, illumination 13h/d~16h/d, relative humidity 75%
~80%.The aerosol index spent a kind of sedge to rot and remove remaining in time is prevented in incubation.
2.3 garlics bloom pollination
Culture implements artificial pollination to when garlic bud is open, pollen scatters.The flower that scatters is removed with tip tweezers when pollination
The anther of powder, on then column cap that the pollen on anther is awarded to different buds, what column cap cannot extend, it needs to break into two with one's hands on petal
Pollination, guarantees to award on the column cap of pollen and is stained with pollen.Pollination operation refers to CN1656883A.
2.4 Ovary cultures, acquisition garlic seed
Referring to CN102257966A method culture garlic ovary after artificial pollination.It is full to observe that garlic ovary expands, plants
(10d~50d, average out to 30d are cultivated from after artificial pollination) when son develops into black, clip garlic ovary.
Garlic ovary is aseptically used 75% alcohol impregnate 1s, aseptic water washing 3 times, 1%~1.5% time
Sodium chlorate solution sterilize 8min~15min, aseptic water washing 3 times.Garlic ovary is aseptically peeled off, by black garlic kind
Son is stripped out from ovary.
The emergence culture of 2.5 garlic seedlings
It is seeded on No. 1 culture medium of seed from the garlic fresh seeds being stripped out in ovary, each seed is individually put
It puts.15d~30d is cultivated under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d.
No. 2 culture mediums of seed are set in switching, and 15d~30d is cultivated under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d.
No. 3 culture mediums of seed are set in switching, and culture to seed is sprouted under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d
Issue seedling.
3, data statistics
The germinating to seedling rate of garlic G398, garlic 09-3 seed, respectively 77%~83%, 75% are counted after culture 120d
~82%, average value is all larger than 80%.
Embodiment two
It is emerged with the method for the present invention culture garlic cultivar seedling.
1, material agents
1.1 garlic plant materials are the same as embodiment one.
1.2 culture medium
Flower a kind of sedge phase nutrient solution is the same as embodiment one.
B5 basal medium is the same as embodiment one.Seed 1, No. 2, No. No. 3 culture mediums be into 1LB5 basal medium point
Not Tian Jia component shown in table 2, and adjust pH to 5.8~6.0, high pressure sterilization is spare.
2 embodiment of table, two seed 1, No. 2, addO-on therapy in No. 3 culture mediums
2, culture operation
2.1~2.4 the same as embodiment one.
2.5 garlic seedling cultures
The garlic seed being stripped out from ovary is seeded in No. 1 culture medium of seed, cultivates under the conditions of same embodiment one
15d~20d.
No. 2 culture mediums of seed are set in switching, and 15d~25d is cultivated under the conditions of same embodiment one.
No. 3 culture mediums of seed are set in switching, and culture to seed sprouts seedling out under the conditions of same embodiment one.
3, data statistics
The germinating to seedling rate of garlic G398, garlic 09-3 seed, respectively 81%~83%, 80% are counted after culture 100d
~82%.
Embodiment three
It is emerged with the method for the present invention culture garlic cultivar seedling.
1, material agents
1.1 garlic plant materials are the same as embodiment one.
1.2 culture medium
Flower a kind of sedge phase nutrient solution is the same as embodiment one.
B5 basal medium is the same as embodiment one.Seed 1, No. 2, No. No. 3 culture mediums be into 1LB5 basal medium point
Not Tian Jia component shown in table 3, and adjust pH to 5.8~6.0, high pressure sterilization is spare.
3 embodiment of table, three seed 1, No. 2, addO-on therapy in No. 3 culture mediums
2, culture operation
2.1~2.4 the same as embodiment one.
2.5 garlic seedling cultures
The garlic seed being stripped out from ovary is seeded in No. 1 culture medium of seed, cultivates under the conditions of same embodiment one
20d~25d.
No. 2 culture mediums of seed are set in switching, and 15d~30d is cultivated under the conditions of same embodiment one.
No. 3 culture mediums of seed are set in switching, and culture to seed sprouts seedling out under the conditions of same embodiment one.
3, data statistics
The germinating to seedling rate of garlic G398, garlic 09-3 seed, respectively 79%~81%, 79% are counted after culture 100d
~80%.
Example IV
It is emerged with the method for the present invention culture garlic cultivar seedling.
1, material agents
1.1 garlic plant materials are the same as embodiment one.
1.2 culture medium
Flower a kind of sedge phase nutrient solution is the same as embodiment one.
B5 basal medium is the same as embodiment one.Seed 1, No. 2, No. No. 3 culture mediums be into 1LB5 basal medium point
Not Tian Jia component shown in table 4, and adjust pH to 5.8~6.0, high pressure sterilization is spare.
4 example IV seed of table 1, No. 2, addO-on therapy in No. 3 culture mediums
2, culture operation
2.1~2.4 the same as embodiment one.
2.5 garlic seedling cultures
The garlic seed being stripped out from ovary is seeded in No. 1 culture medium of seed, cultivates under the conditions of same embodiment one
20d~25d.
No. 2 culture mediums of seed are set in switching, and 15d~30d is cultivated under the conditions of same embodiment one.
No. 3 culture mediums of seed are set in switching, and culture to seed sprouts seedling out under the conditions of same embodiment one.
3, data statistics
The germinating to seedling rate of garlic G398, garlic 09-3 seed, respectively 77%~79%, 75% are counted after culture 100d
~78%.
Embodiment five
It is emerged with the method for the present invention culture garlic cultivar seedling.
1, material agents
1.1 garlic plant materials are the same as embodiment one.
1.2 culture medium
Flower a kind of sedge phase nutrient solution is the same as embodiment one.
B5 basal medium is the same as embodiment one.Seed 1, No. 2, No. No. 3 culture mediums be into 1LB5 basal medium point
Not Tian Jia component shown in table 5, and adjust pH to 5.8~6.0, high pressure sterilization is spare.
5 embodiment of table, five seed 1, No. 2, addO-on therapy in No. 3 culture mediums
2, culture operation
2.1~2.4 the same as embodiment one.
2.5 garlic seedling cultures
The garlic seed being stripped out from ovary is seeded in No. 1 culture medium of seed, cultivates under the conditions of same embodiment one
15d~25d.
No. 2 culture mediums of seed are set in switching, and 15d~30d is cultivated under the conditions of same embodiment one.
No. 3 culture mediums of seed are set in switching, and culture to seed sprouts seedling out under the conditions of same embodiment one.
3, data statistics
The germinating to seedling rate of garlic G398, garlic 09-3 seed, respectively 79%~80%, 78% are counted after culture 100d
~79%.
Claims (9)
1. garlic cultivar seedling germination method, it is characterised in that:
It is seeded in No. 1 culture medium of seed after the fresh seeds of garlic cultivar are sterilized, in 23 ± 2 DEG C of temperature, illumination 13h/d
15d~30d is cultivated under the conditions of~16h/d；No. 2 culture mediums of seed are set in switching, in 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d
Under the conditions of cultivate 15d~30d；No. 3 culture mediums of seed are set in switching, are trained under the conditions of 23 ± 2 DEG C of temperature, illumination 13h/d~16h/d
It supports to seed and sprouts seedling out；The garlic cultivar is the type garlic cultivar of blossoming and having seeds through artificially breeding；
No. 1 culture medium of the seed is addition caseinhydrolysate 100g~800g, glycine in every liter of B5 basal medium
0.5mg~3.0mg, sucrose 25g~60g, every liter agar solidified with 6g~7g, adjusts pH to 5.8~6.0；The seed 2 trainings
Feeding base is addition 6-BA0.01mg~2.00mg, caseinhydrolysate 100g~800g, glycine in every liter of B5 basal medium
0.5mg~3.0mg, sucrose 25g~60g, every liter agar solidified with 6g~7g, adjusts pH to 5.8~6.0；The seed 3 trainings
Feeding base be in every liter of B5 basal medium add 6-BA 0.5mg~2.0mg, NAA0.01mg~1.00mg, sucrose 25g~
45g, every liter agar solidified with 6g~7g, adjusts pH to 5.8~6.0.
2. germination method according to claim 1, it is characterised in that: No. 3 seed 1, seed 2, seed culture medium groups
Dividing is one of following scheme:
Scheme one: No. 1 culture medium of the seed is that caseinhydrolysate 700g~800g, sweet is added in every liter of B5 basal medium
Propylhomoserin 0.5mg~1.0mg, sucrose 50g~60g, No. 2 culture mediums of the seed are to add 6- in every liter of B5 basal medium
BA0.01mg~0.50mg, caseinhydrolysate 400g~600g, glycine 0.5mg~1.0mg, sucrose 35g~45g, described kind
Sub No. 3 culture mediums are addition 6-BA 0.5mg~0.8mg, NAA0.01mg~0.25mg, sucrose in every liter of B5 basal medium
25g~30g；
Scheme two: No. 1 culture medium of the seed is that caseinhydrolysate 350g~500g, sweet is added in every liter of B5 basal medium
Propylhomoserin 1.1mg~1.6mg, sucrose 40g~50g, No. 2 culture mediums of the seed are to add 6- in every liter of B5 basal medium
BA0.51mg~1.00mg, caseinhydrolysate 300g~400g, glycine 1.1mg~1.6mg, sucrose 25g~35g, described kind
Sub No. 3 culture mediums are addition 6-BA 0.9mg~1.2mg, NAA0.26mg~0.50mg, sucrose in every liter of B5 basal medium
31g~35g；
Scheme three: No. 1 culture medium of the seed is that caseinhydrolysate 500g~600g, sweet is added in every liter of B5 basal medium
Propylhomoserin 1.7mg~2.4mg, sucrose 25g~35g, No. 2 culture mediums of the seed are to add 6- in every liter of B5 basal medium
BA1.01mg~1.50mg, caseinhydrolysate 100g~300g, glycine 1.7mg~2.4mg, sucrose 40g~50g, described kind
Sub No. 3 culture mediums are addition 6-BA 1.3mg~1.6mg, NAA0.51mg~0.75mg, sucrose in every liter of B5 basal medium
36g~40g；
Scheme four: No. 1 culture medium of the seed is that caseinhydrolysate 100g~350g, sweet is added in every liter of B5 basal medium
Propylhomoserin 2.5mg~3.0mg, sucrose 35g~45g, No. 2 culture mediums of the seed are to add 6- in every liter of B5 basal medium
BA1.51mg~2.00mg, caseinhydrolysate 600g~800g, glycine 2.5mg~3.0mg, sucrose 50g~60g, described kind
Sub No. 3 culture mediums are addition 6-BA 1.7mg~2.0mg, NAA0.76mg~1.00mg, sucrose in every liter of B5 basal medium
41g~45g.
3. germination method according to claim 2, it is characterised in that:
In the scheme one, material is cultivating 15d~20d on No. 1 culture medium of seed, cultivates 15d on No. 2 culture mediums of seed
~25d；
In the scheme two, material is cultivating 20d~25d on No. 1 culture medium of seed, cultivates 15d on No. 2 culture mediums of seed
~30d；
In the scheme three, material is cultivating 20d~25d on No. 1 culture medium of seed, cultivates 15d on No. 2 culture mediums of seed
~30d；
In the scheme four, material is cultivating 15d~25d on No. 1 culture medium of seed, cultivates 15d on No. 2 culture mediums of seed
~30d.
4. germination method according to claim 1, it is characterised in that: the acquisition of the fresh seeds of the garlic cultivar
It is to cultivate garlic cultivar to flower a kind of sedge phase, cuts flower a kind of sedge with petal, cultivates after rejecting aerosol index to garlic bud
Open, pollen scatters, and implements artificial pollination；It is full to ovary increasing to continue culture, occurs black seeds in ovary；Clip contains
The ovary of black garlic seed；Ovary is sterilized, garlic seed is separated from ovary.
5. germination method according to claim 4, it is characterised in that: flower a kind of sedge phase garlic 2d~18d after existing a kind of sedge, from away from
Cut flower a kind of sedge with petal at the 5cm~25cm of ground, reject aerosol index, be put into after cleaning in colored a kind of sedge phase nutrient solution cultivate to
Garlic bud is open, pollen scatters；Colored a kind of sedge phase nutrient solution is common garlic nutrient liquid.
6. breeding method according to claim 5, it is characterised in that: flower a kind of sedge phase garlic 2d~18d after existing a kind of sedge, to see
It observes aerosol index and bud is grown to index simultaneously, cut flower a kind of sedge with petal.
7. germination method according to claim 4, it is characterised in that: flower a kind of sedge with petal is cultivated in flower a kind of sedge phase nutrient solution
Condition is 25 ± 2 DEG C of temperature, illumination 13h/d~16h/d, relative humidity 75%~80%.
8. germination method according to claim 4, it is characterised in that: removed in time in flower a kind of sedge cultivating process with petal residual
The aerosol index deposited.
9. application of any garlic cultivar seedling germination method of claim 1~8 in garlic crossbreeding.
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