CN1656885A - Technology for cultivation garlic sexual propagation embryo - Google Patents
Technology for cultivation garlic sexual propagation embryo Download PDFInfo
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- CN1656885A CN1656885A CN 200510042524 CN200510042524A CN1656885A CN 1656885 A CN1656885 A CN 1656885A CN 200510042524 CN200510042524 CN 200510042524 CN 200510042524 A CN200510042524 A CN 200510042524A CN 1656885 A CN1656885 A CN 1656885A
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Abstract
A technique for culturing the sexual propagation embryo of garlic to obtain the garlic plant is disclosed. The culture medium for garlic ovaria and the culture medium for sexual embryo are provided.
Description
Technical field
The present invention relates to technology and method that garlic sexual is cultivated, belong to the vegetables sciemtifec and technical sphere.
Background technology
Owing under the conventional cultivation condition, can not normally bloom, set seeds, can not sexual hybridization, so can only lean on kind of a garlic clove vegetative propagation for a long time.Can not carry out sexual propagation, cause garlic genetic background narrow, can't utilize the different garlic germ plasm resource of genetic background to cultivate new varieties; The continuous vegetative propagation of garlic simultaneously causes virus to accumulate, cause garlic kind " degeneration " serious in vivo, and yield and quality descends.Obtain the focus that garlic sexual propagation embryo and complete plant are world technology worker tackling key problem.The research garlic sexual propagation there are important economic implications and learning value.
External report (Etho, 1983) was once collected and can be educated the garlic germplasm, had carried out the research that selfing or hybridization obtained the garlic seed, but but they collect the breeding matter of utilization and belong to wild relatives more, the domestication difficulty more is difficult to utilize.The domestic report of not seeing the garlic sexual propagation success.
Summary of the invention
The invention provides garlic ovary medium and sexual embryo culture base, and the application by this garlic ovary medium and embryo culture base, successfully obtain the breeding method of garlic sexual propagation embryo and whole plant.
Can tissue culture achieve success, and medium is a committed step, below is ovary medium and embryo culture base.
Ovary medium: B5+Macro-+Micro-+Sucrose 40-12g/L
Embryo culture base: MS+BA0.01-0.05mg/L+NAA0.05-0.1mg/L+Sucrose30-60g/L
It below is the operating process of garlic ovary medium and sexual embryo culture base.
Ovary is cultivated operating process:
The garlic ovary of select normal development, obviously expanding, the base portion that connects from scape downcuts, running water flushing 30-40m., 75% alcohol-pickled 1-2s, 0.1% mercuric chloride soaks 3-5m., uses aseptic water washing again 3 times.Wiping the water of doing on the ovary with sterilizing paper on the superclean bench, the ovary otch is downward, be seeded on the ovary medium, put 25 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 70-80%.After cultivating 44-56d, the ovule of ovary has growing in various degree, and the rataria of the different developmental phases of growing is arranged, and what have grows complete embryo.
Embryo is cultivated operating process:
Under the aseptic condition, observe, separate the rataria and the complete embryo of different developmental phases, be seeded on the embryo culture base, put 23 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 65-75% by 40 times of anatomical lens.Rataria is reached maturity after cultivating 44d, and complete embryonic development has become plantlet.
Sucrose (Sucrose) content is the key factor of this medium in the ovary medium of the present invention.The ovary of using this medium culture keeps green, and expands, and the ovule in it continues to grow, and has formed the embryo of different developmental phases.Ovary flavescence on the low medium of other sucrose (Sucrose) concentration is until withered, dead.Sugared concentration and suitable low auxin concentration suitably high in the embryo culture base are beneficial to embryonic development.Embryonic development on the medium that other sucrose (Sucrose) concentration is on the low side and auxin concentration is higher is subjected to the different inhibition of degree, if any arrest of development, " vitrifying " that has etc.The present invention cultivates into complete plant from the garlic embryo of fertilization, need not be through stage of substantive seed, need not cultivation domestication, and can shorten theoretical research and crossbreeding cycle; Be fit to multiple cultivation garlic germplasm, applied range has overcome in the world prior art and has collected germplasm and spend a large amount of human and material resources and time, and range of application is narrow, and the deficiency of domestication difficulty.
Specific embodiment
Now the embodiments of the present invention mode is described with embodiment:
Embodiment 1
The medium that ovary is cultivated: B5+Macro
-+ Micro+Sucrose 26g/L
The medium of sexual embryo culture: MS+BA0.03mg/L+NAA0.07mg/L+Sucrose45/L
The garlic ovary of select normal development, obviously expanding, the base portion that connects from scape downcuts, running water flushing 30m., 75% alcohol-pickled 1s., 0.1% mercuric chloride soaks 3m., uses aseptic water washing again 3 times.Wiping the water of doing on the ovary with sterilizing paper on the superclean bench, the ovary otch is downward, be seeded on the ovary medium, put 25 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 75%.After cultivating 52d, the ovule of ovary has growing in various degree, and the rataria of the different developmental phases of growing is arranged, and what have grows complete embryo.Under the aseptic condition, observe, separate the rataria and the complete embryo of different developmental phases, be seeded on the embryo culture base, put 23 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 65-70% by 40 times of anatomical lens.Rataria is reached maturity after cultivating 50d, and complete embryonic development has become plantlet.
Embodiment 2
The medium that ovary is cultivated: B5+Macro
-+ Micro+Sucrose 40g/L
The medium of sexual embryo culture: MS+BA0.01mg/L+NAA0.02mg/L+Sucrose30/L
The garlic ovary of select normal development, obviously expanding, the base portion that connects from scape downcuts, running water flushing 30m., 75% alcohol-pickled 1s., 0.1% mercuric chloride soaks 3m., uses aseptic water washing again 3 times.Wiping the water of doing on the ovary with sterilizing paper on the superclean bench, the ovary otch is downward, be seeded on the ovary medium, put 25 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 75%.After cultivating 52d, the ovule of ovary has growing in various degree, and the rataria of the different developmental phases of growing is arranged, and what have grows complete embryo.Under the aseptic condition, observe, separate the rataria and the complete embryo of different developmental phases, be seeded on the embryo culture base, put 23 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 65-70% by 40 times of anatomical lens.Rataria is reached maturity after cultivating 44d, and complete embryonic development has become plantlet.
Embodiment 3
The medium that ovary is cultivated: B5+Macro
-+ Micro+Sucrose 12g/L
The medium of sexual embryo culture: MS+BA0.05mg/L+NAA0.1mg/L+Sucrose60/L
The garlic ovary of select normal development, obviously expanding, the base portion that connects from scape downcuts, running water flushing 30m., 75% alcohol-pickled 1s., 0.1% mercuric chloride soaks 3m., uses aseptic water washing again 3 times.Wiping the water of doing on the ovary with sterilizing paper on the superclean bench, the ovary otch is downward, be seeded on the ovary medium, put 25 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 75%.After cultivating 43d, the ovule of ovary has growing in various degree, and the rataria of the different developmental phases of growing is arranged, and what have grows complete embryo.Under the aseptic condition, observe, separate the rataria and the complete embryo of different developmental phases, be seeded on the embryo culture base, put 23 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 65-70% by 40 times of anatomical lens.Rataria is reached maturity after cultivating 45d, and complete embryonic development has become plantlet, and the plant base portion that has expands, and forms clove.
Claims (5)
1. a technology for cultivation garlic sexual propagation embryo is characterized in that preparing ovary medium and embryo culture base, and utilizes medium successfully to obtain the operating process of garlic sexual propagation embryo and whole plant.
2. according to the described technology for cultivation garlic sexual propagation embryo of claim 1, it is characterized in that ovary medium: B5+Macro
-+ Micro+Sucrose 40-12g/L
3. according to the described technology for cultivation garlic sexual propagation embryo of claim 1, it is characterized in that embryo culture base MS+BA0.01-0.05mg/L+NAA0.05-0.1mg/L+Sucrose30-60g/L
4. the operating process of cultivating: with normal development, the garlic ovary of obviously expanding according to claim 1 and 2 described ovarys, the base portion that connects from scape downcuts, running water flushing 30m. (scope), 75% alcohol-pickled 1s (scope), 0.1% mercuric chloride soaks 3-5min., uses aseptic water washing again 3 times.Wiping the water of doing on the ovary with sterilizing paper on the superclean bench, the ovary otch is downward, be seeded on the ovary medium, put 25 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 70-80%.Cultivate 47-56d
5. the operating process of cultivating: under aseptic condition according to claim 1 and 2 described ovarys, observe, separate the rataria and the complete embryo of different developmental phases by 40 times of anatomical lens, be seeded on the embryo culture base, put 23 ± 2 ℃ of temperature, illumination 13-16h/d, the cultivation indoor cultivation of relative moisture 65-75%.Rataria is reached maturity after cultivating 44d, and complete embryonic development has become plantlet.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510042524 CN1656885A (en) | 2005-03-01 | 2005-03-01 | Technology for cultivation garlic sexual propagation embryo |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200510042524 CN1656885A (en) | 2005-03-01 | 2005-03-01 | Technology for cultivation garlic sexual propagation embryo |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396003B (en) * | 2008-08-13 | 2011-03-23 | 山东省农业科学院蔬菜研究所 | Solid culture medium for culturing garlic sexual embryo |
| CN102257966A (en) * | 2011-07-01 | 2011-11-30 | 山东省农业科学院蔬菜研究所 | Method for preventing pollution of garlic ovary culture |
| CN103004596A (en) * | 2012-12-17 | 2013-04-03 | 南京工业大学 | Method for rapidly obtaining garlic seedlings by using intermittent immersion culture mode |
| CN109105236A (en) * | 2018-09-27 | 2019-01-01 | 山东省农业科学院蔬菜花卉研究所 | Garlic cultivar seedling germination method and its application |
-
2005
- 2005-03-01 CN CN 200510042524 patent/CN1656885A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101396003B (en) * | 2008-08-13 | 2011-03-23 | 山东省农业科学院蔬菜研究所 | Solid culture medium for culturing garlic sexual embryo |
| CN102257966A (en) * | 2011-07-01 | 2011-11-30 | 山东省农业科学院蔬菜研究所 | Method for preventing pollution of garlic ovary culture |
| CN102257966B (en) * | 2011-07-01 | 2013-01-16 | 山东省农业科学院蔬菜研究所 | Method for preventing pollution of garlic ovary culture |
| CN103004596A (en) * | 2012-12-17 | 2013-04-03 | 南京工业大学 | Method for rapidly obtaining garlic seedlings by using intermittent immersion culture mode |
| CN103004596B (en) * | 2012-12-17 | 2014-07-16 | 南京工业大学 | Method for rapidly obtaining garlic seedlings by using intermittent immersion culture mode |
| CN109105236A (en) * | 2018-09-27 | 2019-01-01 | 山东省农业科学院蔬菜花卉研究所 | Garlic cultivar seedling germination method and its application |
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