CN109090337A - A kind of additive for microbe feedstuff and preparation method thereof improving cultivated animals immunity of organisms - Google Patents
A kind of additive for microbe feedstuff and preparation method thereof improving cultivated animals immunity of organisms Download PDFInfo
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- CN109090337A CN109090337A CN201810731183.2A CN201810731183A CN109090337A CN 109090337 A CN109090337 A CN 109090337A CN 201810731183 A CN201810731183 A CN 201810731183A CN 109090337 A CN109090337 A CN 109090337A
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- additive
- microbe feedstuff
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The present invention provides a kind of additive for microbe feedstuff for improving cultivated animals immunity of organisms, it is characterized in that, the additive for microbe feedstuff is that the raw material matched by following component and weight percent is formed by composite sync solid fermentation: composite bacteria agent 5-35%, carbon source 2-25%, nitrogen source 5-30%, inorganic salts 0.5-5%, biotin 0.01-0.1%, water 5-35%；The present invention also provides a kind of preparation methods of the additive for microbe feedstuff of above-mentioned raising cultivated animals immunity of organisms.Additive for microbe feedstuff of the invention can effectively promote antibody level in cultivated animals blood circulation, improve cultivated animals immunity of organisms.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff more particularly to one kind can effectively promote cultivated animals circulating
Antibody level, the additive for microbe feedstuff for improving cultivated animals immunity of organisms and preparation method thereof in liquid.
Background technique
Nineteen sixty-five September 17 days, synthetic insulin was found in first Chinese, this is also first protein in the world
It is fully synthetic.This achievement promotes the development of life science, opens the epoch of synthetic protein.This works complete
At, it is considered to be sixties peptide and protein synthesizes the most important achievement in field, greatly improves the section of our countries
Reputation is learned, positive impetus has been risen to research of the China in terms of proteins and peptides synthesis.The conjunction of artificial bovine insulin
At, indicate the mankind understanding life, explore life secret journey in, stepped a critical step, produced and its huge
Big meaning and influence.
Active peptide is the general name of more than 1,000 kinds of peptides (such as soybean peptide, sea cucumber peptide etc. are one of active peptides).It is people's
Growth and development, metabolism, disease and aging play key effect in dead process.
The production method of polypeptide mainly has 3 kinds: (1) being formed by breaks down proteins: including enzymatic isolation method, chemical hydrolysis, micro-
Biological fermentation process.(2) it is extracted from plant or microorganism.(3) free amino acid is synthesized through artificial chemistry.The method of polypeptide production
Respectively there are advantage and disadvantage, the selection of method depends primarily on the length and quantity of required peptide.Extraction method is due to resource is limited and at high cost
It is unfavorable for applying in feed.Chemical synthesis is widely used in the pharmaceutical grade peptide for being short to middle length of production high value, such as hydrolyzed casein
With junket kassinin kinin etc., the disadvantage is that it is at high cost, and during the reaction may be harmful to health and environment.
Relative maturity, domestic and international existing procucts list technology in terms of Production by Microorganism Fermentation peptide, such as soybean peptide.
Enzymatic isolation method can preferably control production since reaction condition is easily controllable, become the main direction of development of peptides products.Research
Show that immune-active peptides have various physiological functions, it can not only enhance the immunocompetence of body, lifting in animal body
The immunoregulation effect wanted；And the proliferation of body lymphocyte can also be stimulated and enhance the phagocytic activity of macrophage, it improves
Resistivity of the body to extraneous pathogen matter.
For cultivated animals feed on existing market due to Easy dosing, Long term Animal often generates generation after eating feed
The case where immunity function decline, easily ill, great loss is caused to operator.
Summary of the invention
The object of the present invention is to provide one kind can effectively promote antibody level in cultivated animals blood circulation, improves cultivation
The additive for microbe feedstuff and preparation method thereof of animal body immunity.
In order to achieve the above object, technical solution of the present invention provides following technical solution:
On the one hand, the present invention provides a kind of additive for microbe feedstuff for improving cultivated animals immunity of organisms, feature
It is, the additive for microbe feedstuff is that the raw material matched by following component and weight percent passes through composite sync solid fermentation
It forms:
In order to further optimize the above technical scheme, technical measures adopted by the present invention further include:
Further, the composite bacteria agent includes lactobacillus plantarum, lactobacillus acidophilus and S. cervisiae.
Further, in the composite bacteria agent each strain according to weight percent composition are as follows:
Lactobacillus plantarum 20-50%
Lactobacillus acidophilus 20-40%
S. cervisiae 20-50%.
Further, the carbon source includes at least one of starch, glucose, molasses, corn flour.
Further, the nitrogen source includes at least one of beancake powder, mucedin, corn pulp.
Further, the inorganic salts include at least one of ammonium salt, nitrate.
On the other hand, the present invention also provides a kind of additive for microbe feedstuff of above-mentioned raising cultivated animals immunity of organisms
Preparation method, include the following steps:
Step 1, the production of hybrid seeds is produced
(1.1), lactobacillus plantarum, lactobacillus acidophilus and S. cervisiae that Culture Collection Center is bought are inoculated in respectively
MRS is supported in base, while adding the biotin of 0.01-0.1% ratio, cultivates 20-25 hours at a temperature of 35-40 DEG C, then
S. cervisiae is inoculated in PDA rouge culture medium, carries out scribing line culture, select growth fast, the apparent saccharomyces cerevisiae of thallus feature,
Lactobacillus plantarum lactobacillus acidophilus, saves in the lab；
(1.2), step (1.1) resulting saccharomyces cerevisiae is transferred in the eggplant bottle of PDA agar medium, resulting object
Lactobacillus and lactobacillus acidophilus are transferred respectively in the eggplant bottle equipped with MRS agar medium, are cultivated at a temperature of 35-40 DEG C
40-50 hours, it is covered with when eggplant bottle surface lawn and when white middle band Huang can be taken off, be put into 2-6 DEG C of refrigerator and protected
It deposits；
Step 2, ferment making
(2.1), by the step 1 obtained strain of culture by following weight percent with taking: it is lactobacillus plantarum 20-50%, thermophilic
Lactobacillus lactis 20-40%, S. cervisiae 20-50%, obtain composite bacteria agent；
(2.2), (2.1) weighed strain is added by the 5-20% of composite bacteria agent weight percent in composite bacteria agent
In culture medium, fermentation obtains compound microorganism ferments liquid；
Step 3, prepared by finished product
(3.1), the compound microorganism ferments liquid obtained step 2 fermentation is 12-15 hours dry at 40-45 DEG C, dry
Product is crushed afterwards, and smashed product crosses 80 meshes, and the coarse fodder sifted out is crushed and is sieved again, obtains fermented product；
(3.2), the fermented product is packed, obtains finished product additive for microbe feedstuff.
In order to further optimize the above technical scheme, technical measures adopted by the present invention further include:
Further, composite bacteria agent culture medium example by weight percentage are as follows: molasses 0.5-1%, glucose 1-2%,
Sodium chloride 0-1%, beancake powder 10-20%, mucedin 5-15%, corn pulp 3-10%, magnesium sulfate 0-1%, biotin 0.01-
0.1%, water 50-80.5%.
Further, the fermentation condition in (2.2) of the step 2 are as follows: pH value is adjusted into 6-8, is put into container and heats
Sterilizing 20-40 minutes is carried out to 110-130 DEG C, ferments 2-3 days, is fermenting in inoculation fermentation tank after being cooled to 25-35 DEG C
It samples in the process three times, detects pH value 3.2-4.
Further, the finished product in (3.2) of the step 3 is pale yellow powder shape solid, detection parameters are as follows: moisture content≤
12.0%, viable count >=5,000,000,000/g, content of peptides >=25%.
Strain of the present invention is to be commercially available from Culture Collection Center, corresponding deposit number are as follows: plant cream bar
Bacterium Lactobacillus plantarum (ATCC8014), lactobacillus acidophilus Lactobacillusacidophilus
(ATCC4356), S. cervisiae Saccharomyces cerevisiae (IFO0203).
Soya-bean polypeptides, the gluten polypeptide obtained using above-mentioned strain fermentation beancake powder of the invention, mucedin, corn pulp
The in vitro PBL proliferation of the piglet that can stimulate PHA to activate with Semen Maydis polypeptide；In additive for microbe feedstuff of the invention also have Ah
The rice elastoidin (Oryzatensin, Gly-Tyr-Pro-Met-Tyr-Pro-Leu-Pro-Arg) of piece antagonistic activity, can
Enhance the phagocytic function of people PMNL by complement C 3 receptor pathway, people's peripheral leukocytes is promoted to generate O2-, mice spleen leaching can be improved
Bar cell Proliferation, the phagocytic activity of peritoneal macrophage and NK cell activity promote Thl response and macrophage to generate H2O2；This
Invention can promote cultivated animals Development of Immune Organs, remove free radical, effectively prevent animal due to free radical infringement, immune function
It stress caused by a variety of difficult and complicated cases and many reasons caused by capable of lowly waiting.
After beneficial microbe in additive for microbe feedstuff of the invention enters animal and bird intestines, can have in conjunction with original
Beneficial bacteria quickly forms superior microorganism flora, inhibits the growth of harmful intestinal tract bacteria, beneficial microbe therein is around intestinal walls
It is combined closely by teichoic acid and intestinal epithelial cell, forms mycoderm barrier, prevent the field planting of harmful bacteria；Complex microorganism is also
It can produce antibacterial material, lactolin, bacitracin etc., inhibit the harmful bacterias such as Escherichia coli, salmonella numerous in enteron aisle
It grows, be colonized and adsorb, neutralize toxic product, inhibit the synthesis of ammonia and amine, promote the highly reactive energy of gut-associated lymphoid tissue
Power.
For product of the present invention using carbon source, nitrogen source, inorganic salts and casein as Spawn incubation raw material, they are that microorganism is thin
The important component of born of the same parents' structure and enzyme can also maintain the activity of enzyme, can promote microbial growth, stimulating activity enzyme and small
The generation of peptide.
The present invention starts with from the purpose that ecological ideas cultivates, and using agricultural raw material as culture medium, is fermented by multistage enrichment,
Being beneficial to Multiple components raising animal body immune factor in feed for high concentration is generated, while generating a large amount of beneficial bacteriums and promoting
Plant fiber decomposes the additive of the substances such as multiple active enzymes and small peptide, directly adds in feed, it is good can to form animal body
Property ecological environment, is a kind of safe and efficient green feed additive.
The present invention by adopting the above technical scheme, compared with prior art, has the following technical effect that
1) additive for microbe feedstuff of the invention is capable of the proliferation of effective stimulus animal body lymphocyte and is enhanced huge
Cytophilic phagocytic activity helps to improve body to the resistivity of extraneous pathogen matter.
2) Development of Immune Organs that can be improved animal makes body quickly generate antibody, improves antibody titer, generated
Antibody more uniformly and is able to maintain that higher level, and compared to antibody level under normal circumstances, immunopotentiation is bright
It is aobvious.
3) lactic acid bacteria used in can improve the enteron aisle digestion and absorption function of animal body, and it is dynamic that saccharomycete can improve synthesis egg
Single cell protein needed for object orgotein, the present invention help to ensure that animal intestinal tract health by a variety of flora comprehensive functions.
4) it can significantly improve the state of mind of illness livestock and poultry, improve the feed intake of illness livestock and poultry.
5) there is significant growth promoting function to young animal.
6) additive for microbe feedstuff of the invention is fermented using the strain of 2045 bulletins in feed industry, is being guaranteed
Improve its own immune system and growth and development in the case where livestock and poultry animal nutritional need, in animal body without any antibiotic and
Heavy-metal residual meets the requirement of the ecological agriculture, ensure that the health of eater.
Specific embodiment
Technical solution of the present invention is described further below in conjunction with specific embodiment.
Embodiment 1
Step 1, the production of hybrid seeds is produced
(1.1), lactobacillus plantarum, lactobacillus acidophilus and S. cervisiae that Culture Collection Center is bought are inoculated in respectively
MRS is supported in base, while adding the biotin of 0.05% ratio, is cultivated 24 hours at a temperature of 35 DEG C, then by S. cervisiae
It is inoculated in PDA rouge culture medium, carries out scribing line culture, it is thermophilic to select growth fast, the apparent saccharomyces cerevisiae of thallus feature, lactobacillus plantarum
Lactobacillus lactis saves in the lab；
(1.2), the saccharomyces cerevisiae of step (1.1) is transferred in the eggplant bottle of PDA agar medium, lactobacillus plantarum and
Lactobacillus acidophilus is transferred respectively in the eggplant bottle equipped with MRS agar medium, is cultivated 40 hours at a temperature of 35 DEG C, to eggplant
Bottle surface lawn is covered with, and can be taken off when white middle band Huang, is then placed in 2-6 DEG C of refrigerator and is saved；The present invention uses
Following strain: lactobacillus plantarum Lactobacillus plantarum (ATCC8014), lactobacillus acidophilus Lactobacillus
Acidophilus (ATCC4356), S. cervisiae Saccharomyces cerevisiae (IFO0203)；
Step 2, ferment making
(2.1), it is weighed in eggplant bottle by following weight proportion and respectively produces strain: lactobacillus plantarum 20%, lactobacillus acidophilus
40%, S. cervisiae 40%；
(2.2), production strain is mixed, by mixed strain by 15% addition of composite bacteria agent weight ratio by sugar
Sweet 0.75%, glucose 1.5%, sodium chloride 0.8%, beancake powder 15%, mucedin 8%, corn pulp 5%, magnesium sulfate
0.01%, biotin 0.01%, water 68.93% form composite bacteria agent culture medium in, adjust pH value to 6.8, be put into container
It is heated up to 121 DEG C and carries out sterilizing 25 minutes, ferment 2-3 days in inoculation fermentation tank after being cooled to 30 DEG C；It is sampled in fermentation process
Three times, pH value 3.3 is detected；
Step 3, prepared by finished product
(3.1), the product fermented is 13 hours dry at 40 DEG C, product is crushed after drying, smashed production
Product cross 80 meshes, and the coarse fodder sifted out is crushed and is sieved again；
(3.2), it is fitted into packaging bag after being sieved, is transferred to warehouse for finished product and orderly stacks, notify quality control department sample detection, mainly
Parameter: moisture content≤12.0%, viable count >=8,000,000,000/g, content of peptides >=25%；Physical behavior: pale yellow powder shape solid.
Embodiment 2
Step 1, the production of hybrid seeds is produced
(1.1), lactobacillus plantarum, lactobacillus acidophilus and S. cervisiae that Culture Collection Center is bought are inoculated in respectively
MRS is supported in base, while adding the biotin of 0.05% ratio, is cultivated 22 hours at a temperature of 37 DEG C, then by S. cervisiae
It is inoculated in PDA rouge culture medium, carries out scribing line culture, it is thermophilic to select growth fast, the apparent saccharomyces cerevisiae of thallus feature, lactobacillus plantarum
Lactobacillus lactis saves in the lab；
(1.2), the saccharomyces cerevisiae of step (1.1) is transferred in the eggplant bottle of PDA agar medium, lactobacillus plantarum and
Lactobacillus acidophilus is transferred respectively in the eggplant bottle equipped with MRS agar medium, is cultivated 42 hours at a temperature of 37 DEG C, to eggplant
Bottle surface lawn is covered with, and can be taken off when white middle band Huang, is then placed in 2-6 DEG C of refrigerator and is saved；
Step 2, ferment making
(2.1), it is weighed in eggplant bottle by following weight proportion and respectively produces strain: lactobacillus plantarum 30%, lactobacillus acidophilus
30%, S. cervisiae 40%；
(2.2), production strain is mixed, by mixed strain by 12% addition of composite bacteria agent weight ratio by sugar
Sweet 0.1%, glucose 2.0%, sodium chloride 0.9%, beancake powder 12%, mucedin 10%, corn pulp 8%, magnesium sulfate
0.01%, biotin 0.01%, water 66.98% form composite bacteria agent culture medium in, adjust pH value to 7.2, be put into container
It is heated up to 121 DEG C and carries out sterilizing 30 minutes, ferment 2-3 days in inoculation fermentation tank after being cooled to 30 DEG C；It is sampled in fermentation process
Three times, pH value 3.5 is detected；
Step 3, prepared by finished product
(3.1), the product fermented is 13 hours dry at 40 DEG C, product is crushed after drying, smashed production
Product cross 80 meshes, and the coarse fodder sifted out is crushed and is sieved again；
(3.2), it is fitted into packaging bag after being sieved, is transferred to warehouse for finished product and orderly stacks, notify quality control department sample detection, mainly
Parameter: moisture content≤12.0%, viable count >=10,000,000,000/g, content of peptides >=25%；Physical behavior: pale yellow powder shape solid.
Embodiment 3
The present embodiment is verifying embodiment.
Additive for microbe feedstuff of the invention is added in egg feedstuff and is stirred evenly in ‰ ratio of 0.5-1.0,
This product closed at dry and ventilated need to seal up for safekeeping, should use as early as possible behind Kaifeng.
Experimental subjects: the laying hen that feed research and development centre of Shanghai academy of agricultural sciences carries out feeds comparative experiments, by close growth
The laying hen of state is divided into 3 groups, respectively the common chicken feed of feeding and the chicken feed for being added to additive for microbe feedstuff of the invention.
Experimental method: the common chicken feed of control group feeding；1 feeding of experimental group is added to 0.5 ‰ microorganism of the invention
The chicken feed of feed addictive, 2 feeding of experimental group are added to the chicken feed of 1 ‰ additive for microbe feedstuff of the invention；Hello
Food 3 weeks measures weekly IgG content in Laying-hen Serum, and specific experiment the results are shown in Table 1:
Influence (mg/ml) of the 1 antimicrobial polypeptide preparation of table to Laying-hen Serum IgG content
It can see by the measurement data of table 1, after a week compared to control group, IgG contains in Laying-hen Serum in experimental group 2
Amount improves 64.81%；Compared to control group after two weeks, IgG content mentions respectively in Laying-hen Serum in experimental group 1 and experimental group 2
It is high by 37.79% and 79.90%；Compared to control group after two weeks, IgG content point in Laying-hen Serum in experimental group 1 and experimental group 2
56.60% and 102.12% are not improved.Above-mentioned data explanation, additive for microbe feedstuff of the invention can significantly improve egg
The immunocompetence of chicken.Meanwhile additive for microbe feedstuff of the invention can also increase every Day feeding amount of laying hen.
Embodiment 4
The present embodiment is verifying embodiment.
Additive for microbe feedstuff of the invention is added in egg feedstuff and is stirred evenly, this production in 1.0 ‰ ratios
Product closed at dry and ventilated need to be sealed up for safekeeping, should use as early as possible behind Kaifeng.
Experimental subjects: the laying hen that feed research and development centre of Shanghai academy of agricultural sciences carries out feeds comparative experiments, by close growth
The laying hen of state is divided into 2 groups, respectively the common chicken feed of feeding and the chicken feed for being added to additive for microbe feedstuff of the invention.
Experimental method: the common chicken feed of control group feeding；Experimental group feeding is added to 1 ‰ microbiological feed of the invention
The chicken feed of additive；Thymus index, index and spleen index and bursal index, specific experiment are measured after feeding 2 weeks the results are shown in Table 2.
2 microorganism of table raises influence (g/kg) of the polypeptide formulations to laying hen Immune Organs Index
It can see by the measurement data of table 2, after feeding 2 weeks, compared to control group, experimental group thymus index, spleen
Index and bursal index have been respectively increased 66.52%, 40.72% and 36.94%.Above-mentioned data explanation, micro- life of the invention
Object feed addictive can significantly improve the immunocompetence of laying hen.Meanwhile additive for microbe feedstuff of the invention can also increase
Add every Day feeding amount of laying hen.
Embodiment 5
The present embodiment is verifying embodiment.
Additive for microbe feedstuff of the invention is added in pannage and is stirred evenly in 1.0 ‰ ratios, this product
It closed at dry and ventilated need to seal up for safekeeping, should be used as early as possible behind Kaifeng.
Experimental subjects: the piglet of close growth conditions is divided into 2 by the piglet immunological comparative experiments that the Fengxian pig farm Hong Miao carries out
Group the common pannage of feeding and is added to the pannage of additive for microbe feedstuff of the invention respectively.
Experimental method: the common pannage of control group feeding；Experimental group feeding is added to 1 ‰ microbiological feed of the invention
The pannage of additive；Piglet thymus gland, spleen and inguinal lymph nodes index, specific experiment are measured after feeding 3 weeks the results are shown in Table 3.
Influence (g/kg) of the 3 antimicrobial polypeptide preparation of table to piglet immunological tissue and organ weight
It can see by the measurement data of table 3, after feeding 3 weeks, compared to control group, piglet thymus gland, spleen and abdomen stock
The absolute weight of ditch lymph node slightly increases compared with control group, increases by 20.00%, 29.34% and 48.37% compared with control group respectively.On
Data explanation is stated, additive for microbe feedstuff of the invention can significantly improve the development of the immune organ of piglet, improve piglet
Immunocompetence.Meanwhile additive for microbe feedstuff of the invention can also increase every Day feeding amount of piglet.
The growth and development of cultivated animals can be promoted by showing additive for microbe feedstuff of the invention by above-described embodiment,
Improve the immunocompetence of cultivated animals.
Specific embodiments of the present invention are described in detail above, but it is merely an example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, any couple of present invention carries out equivalent modifications and
Substitution is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of additive for microbe feedstuff for improving cultivated animals immunity of organisms, which is characterized in that the microbiological feed
Additive is that the raw material matched by following component and weight percent is formed by composite sync solid fermentation:
2. additive for microbe feedstuff according to claim 1, which is characterized in that the composite bacteria agent includes plant cream bar
Bacterium, lactobacillus acidophilus and S. cervisiae.
3. additive for microbe feedstuff according to claim 2, which is characterized in that each strain is pressed in the composite bacteria agent
According to the composition of weight percent are as follows:
Lactobacillus plantarum 20-50%
Lactobacillus acidophilus 20-40%
S. cervisiae 20-50%.
4. additive for microbe feedstuff according to claim 1, which is characterized in that the carbon source include starch, glucose,
At least one of molasses, corn flour.
5. additive for microbe feedstuff according to claim 1, which is characterized in that the nitrogen source includes beancake powder, gluten
At least one of albumen, corn pulp.
6. additive for microbe feedstuff according to claim 1, which is characterized in that the inorganic salts include ammonium salt, nitric acid
At least one of salt.
7. a kind of preparation method of such as additive for microbe feedstuff of any of claims 1-6, which is characterized in that packet
Include following steps:
Step 1, the production of hybrid seeds is produced
(1.1), lactobacillus plantarum, lactobacillus acidophilus and S. cervisiae that Culture Collection Center is bought are inoculated in MRS respectively
It supports in base, while adding the biotin of 0.01-0.1% ratio, cultivate at a temperature of 35-40 DEG C 20-25 hours, then will make
Brewer yeast bacterium is inoculated in PDA rouge culture medium, carries out scribing line culture, selects and grows fast, the apparent saccharomyces cerevisiae of thallus feature, plant
Lactobacillus lactobacillus acidophilus, saves in the lab；
(1.2), step (1.1) resulting saccharomyces cerevisiae is transferred in the eggplant bottle of PDA agar medium, resulting object cream bar
Bacterium and lactobacillus acidophilus are transferred respectively in the eggplant bottle equipped with MRS agar medium, cultivate 40- at a temperature of 35-40 DEG C
50 hours, it is covered with when eggplant bottle surface lawn and when white middle band Huang can be taken off, be put into 2-6 DEG C of refrigerator and saved；
Step 2, ferment making
(2.1), the strain that step 1 culture obtains is matched by following weight percent and is taken: lactobacillus plantarum 20-50%, acidophilus cream
Bacillus 20-40%, S. cervisiae 20-50%, obtain composite bacteria agent；
(2.2), (2.1) weighed strain is added by the 5-20% of composite bacteria agent weight percent in composite bacteria agent culture
In base, fermentation obtains compound microorganism ferments liquid；
Step 3, prepared by finished product
(3.1), the compound microorganism ferments liquid obtained step 2 fermentation is 12-15 hours dry at 40-45 DEG C, produces after dry
Product are crushed, and smashed product crosses 80 meshes, and the coarse fodder sifted out is crushed and is sieved again, obtain fermented product；
(3.2), the fermented product is packed, obtains finished product additive for microbe feedstuff.
8. preparation method according to claim 7, which is characterized in that composite bacteria agent culture medium example by weight percentage
Are as follows: molasses 0.5-1%, glucose 1-2%, sodium chloride 0-1%, beancake powder 10-20%, mucedin 5-15%, corn pulp 3-
10%, magnesium sulfate 0-1%, biotin 0.01-0.1%, water 50-80.5%.
9. preparation method according to claim 7, which is characterized in that the fermentation condition in (2.2) of the step 2 are as follows:
PH value is adjusted into 6-8, be put into container be heated up to 110-130 DEG C carry out sterilizing 20-40 minutes, after being cooled to 25-35 DEG C
It ferments 2-3 days in inoculation fermentation tank, samples during the fermentation three times, detect pH value 3.2-4.
10. preparation method according to claim 7, which is characterized in that the finished product in (3.2) of the step 3 is yellowish
Color pulverulent solids, detection parameters are as follows: moisture content≤12.0%, viable count >=5,000,000,000/g, content of peptides >=25%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931682A (en) * | 2020-12-29 | 2021-06-11 | 南京惠牧生物科技有限公司 | Microbial feed additive and preparation method thereof |
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