CN109082394A - A kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust - Google Patents

A kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust Download PDF

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Publication number
CN109082394A
CN109082394A CN201810950317.XA CN201810950317A CN109082394A CN 109082394 A CN109082394 A CN 109082394A CN 201810950317 A CN201810950317 A CN 201810950317A CN 109082394 A CN109082394 A CN 109082394A
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dissolved oxygen
parts
ammonia nitrogen
preparation
activation
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巩华
张洪业
朱宇航
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Guangzhou Haicheng Biotechnology Co Ltd
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Guangzhou Haicheng Biotechnology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry

Abstract

The present invention provides a kind of leavening that can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted, the leavening include bacterial content account for the nitrobacteria of total bacteria count 10%~30%, 30%~50% bacillus, 20%~40% lactic acid bacteria, 5%~15% yeast.The present invention also provides it is a kind of can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted with and preparation method thereof, the biological agent is mainly by the above-mentioned activated post-fermentation raw material preparation gained of leavening.The present invention is prepared into biological agent by reasonably combined a variety of probiotics fermentation raw materials, cooperate to obtain a kind of biological agent for effectively adjusting pond ammonia nitrogen and dissolved oxygen using different strain, the preparation method of said preparation is simple, it is not high to equipment requirement, strong operability is promoted the use of easily in scale raising or in aquaculture cooperative society.

Description

A kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust
Technical field
The present invention relates to aquaculture water quality regulation and control fields, and in particular to a kind of to can be used for what pond ammonia nitrogen and dissolved oxygen were adjusted Complex microorganism preparations.
Background technique
Studies have shown that when the cultured fishes load in pond is more than 333 kgs/acre, the method for traditional " supporting algae water transfer " Do not have an effect of regulating water quality, and the Pearl River Delta area pond per unit area yield that is averaged is 700 kgs/acre, some cultivation hybridization lifes Fish reaches as high as 30000 kgs/acre.Such highdensity aquaculture is easy to cause excessive bait throwing in, excreta accumulation, thus Cause ammonia nitrogen, content of nitrite exceeded.
Nitrite has been to endanger one of most important water body murder by poisoning factor of aquatic products health, gently then influences the life of aquatic products It is long, it is heavy then lead to morbidity or death.The presence of nitrite easily make aquaculture pond occur the phenomenon of falling algae, occur water colour overrich, nigrescence, Aging phenomena such as rubescent, muddy, and then breed toxic pathogenic bacteria cause to cultivate object morbidity and even die of illness, such as the red leg, blind of shrimp Eye, rotten limb phenomenon；The black gill of ocean fish and fresh-water fishes, the gill be swollen, gill rot, blind, it is disconnected must phenomena such as；Under the survival rates such as sea cucumber, shellfish Drop；And then lead to the hypoimmunity of biology, cultured output is influenced, cultivation risk is brought, is caused damages.
Ammonia nitrogen in breeding water body, nitrite treatments at present mainly use physics, chemistry and biological means (Wheaton；Lin et al,2002).And physical processing techniques are current research and apply more processing technique, it is main to wrap Include the solid-liquid separation technique (Wheaton based on mechanical filter (gravity flow screen, rotary screen, vibrating screen, sand filter), gravitational settling； Lin et al, 2002), foam separating technology (Wheaton；Lin et al, 2002) and adsorption technology (Ali et al, 2005) etc..Mechanical filter technology has the advantages that cost and operating cost are low etc. in physical treatment facility, but can only remove cultivation Suspended matter in waste water can only remove a small amount of dissolubility pollutant by foam separating technology.
In chemical treatment method, ozonation technology more application is in intensive culture wastewater treatment, ozone oxidation energy Power is strong, not only can quickly kill virus, bacterium and microalgae in water body, but also can decompose organic matter and ammonia, nitrous in water body The reducing inorganics such as hydrochlorate matter (Suantika et al, 2001).But ozone treatment cost is relatively high, and ozone residual is for supporting Growing object has certain toxic side effect (Martin et al, 2003).Compared to ozone treatment mode, ultraviolet irradiation processing is one The water body processing method of kind lower cost, but its main function is sterilization, and due to by ultraviolet light penetration capacity and water body The influence of irradiation time is stopped, the bactericidal effect of ultraviolet irradiation processing is not so good as ozone.
Biological treatment is a kind of processing technique risen this nearly year, soluble albumen, carbon hydrate in breeding wastewater The NH of object, fatty acid and inorganic states4 +、NO2 -、NO3 -Salt, these organic matters and inorganic matter are suitable for the side with biological treatment Method removal.Due to being influenced by biological interaction, biological species and biological self reproducing metabolic rule, the validity of biological treatment It once fell under suspicion with stability, but with the progress of biotechnology and the relevant technologies, the validity and stabilization of biological treatment Property is constantly enhanced.Still microbiological treatment technology (Stams et currently most in the application of biological wastewater treatment field Al, 1997), the diversification of microbe species and metabolic function is utilized in microbiological treatment, sufficiently excavation microbial resources.Micro- life The principle of object processing is: using the albumen, carbohydrate, fatty acid of heterotrophicy bacteria mineralising solubility at inorganic salts, utilizing Nitrobacteria is by NH4 +、NO2 -It is converted into the NO of low toxicity3 -, recycle denitrifying bacteria by NO3 -Denitrogenation is to N2.Breeding wastewater at present In biochemical treatment using it is more be bio-contact oxidation (Liao Shaoan etc., 2008), biological rotating disk (Brazil, 2006), biology The techniques such as fluidized bed (Bortone et al, 2003), biofacies is greatly mostly from nature or artificial environment in these processes Ligotrophic group, does not adapt to the nutrient environment of breeding wastewater, and the unit volume processing load of microbiological treatment module is practical not Height is greatly affected the application of traditional microbiological Treatment process.
Summary of the invention
In view of this, the present invention provides a kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust, the life Compound formulation is made by the scientific matching using nitrobacteria, bacillus, lactic acid bacteria and yeast in object preparation, and said preparation is exchanged Saving pond ammonia nitrogen and dissolved oxygen has good effect, and easy to use, and effect is obvious.
To adjust pond ammonia nitrogen and dissolved oxygen, first aspect present invention, which provides one kind, can be used for pond ammonia nitrogen and dissolved oxygen adjusting Complex microorganism preparations leavening, including compound bacteria A and compound bacteria B；
Wherein, the compound bacteria A includes one of nitrobacteria, bacillus or a variety of；The compound bacteria B includes cream One of sour bacterium, yeast are a variety of.
In an embodiment of the present invention, when a kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust Leavening when including nitrobacteria, bacillus, lactic acid bacteria and yeast, the nitrobacteria content accounts for the percentage of total bacteria count It is 10%~30%；The percentage that the bacillus content accounts for total bacteria count is 30%~50%；The content of lactic acid bacteria accounts for always The percentage of bacterium number is 20%~40%；The percentage that the amount of yeast accounts for total bacteria count is 5%~15%.
In a preferred embodiment, when a kind of complex microorganism that can be used for pond ammonia nitrogen and dissolved oxygen and adjust When the leavening of preparation includes nitrobacteria, bacillus, lactic acid bacteria and yeast, the nitrobacteria content accounts for the hundred of total bacteria count Divide than being 20%；The percentage that the bacillus content accounts for total bacteria count is 40%；The content of lactic acid bacteria accounts for the hundred of total bacteria count Divide than being 30%；The percentage that the amount of yeast accounts for total bacteria count is 10%.
It is understood that those skilled in the art can be according to strain formula provided by the invention, bacterium according to routine Additive amount (mass percent of bacterium is 1~2%) is added corresponding culture medium and is mixed and made into bacterium solution or bacterium powder, is specifically commercially answering In, the leavening for the complex microorganism preparations that the present invention protected can be used for pond ammonia nitrogen and dissolved oxygen is adjusted can be bacterium solution or Bacterium powder.
In an embodiment of the present invention, the present invention provides a kind of composite microbials that can be used for pond ammonia nitrogen and dissolved oxygen and adjust The leavening of object preparation, what the leavening included that can be used for of providing of first aspect present invention, pond ammonia nitrogen and dissolved oxygen adjusted answers The leavening for closing microorganism formulation, further includes fluid nutrient medium, solid medium or powdered medium.
Second aspect of the present invention provides a kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust, mainly The activated post-fermentation raw material preparation gained of leavening provided by first aspect present invention, wherein the raw material includes that weight is matched Than the dregs of beans and wheat bran for 1:1.
In an embodiment of the present invention, the complex microorganism preparations, including compound bacteria A and compound bacteria B；
Wherein, the compound bacteria A includes one of nitrobacteria, bacillus or a variety of；The compound bacteria B includes cream One of sour bacterium, yeast are a variety of.
In an embodiment of the present invention, when a kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust Leavening when including nitrobacteria, bacillus, lactic acid bacteria and yeast, the nitrobacteria content accounts for the percentage of total bacteria count It is 10%~30%；The percentage that the bacillus content accounts for total bacteria count is 30%~50%；The content of lactic acid bacteria accounts for always The percentage of bacterium number is 20%~40%；The percentage that the amount of yeast accounts for total bacteria count is 5%~15%.
In a preferred embodiment, when a kind of complex microorganism that can be used for pond ammonia nitrogen and dissolved oxygen and adjust When the leavening of preparation includes nitrobacteria, bacillus, lactic acid bacteria and yeast, the nitrobacteria content accounts for the hundred of total bacteria count Divide than being 20%；The percentage that the bacillus content accounts for total bacteria count is 40%；The content of lactic acid bacteria accounts for the hundred of total bacteria count Divide than being 30%；The percentage that the amount of yeast accounts for total bacteria count is 10%.
In an embodiment of the present invention, leavening activation activation medium used includes the group of following parts by weight Point: 1000 parts of cultivating pool water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of epsom salt, 0.5 part of dipotassium hydrogen phosphate, carbon 14.29 parts of sour calcium, 2 parts of sodium chloride, 1 part of brine, 0.02 part of a water calcium chloride, 0.05 part of ferrous sulfate heptahydrate, cupric sulfate pentahydrate 0.05 part.
Third aspect present invention provides a kind of system that can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted Preparation Method, comprising the following steps:
(1) pretreatment of fermentation raw material: dregs of beans and wheat bran that weight proportion is 1:1 are crushed, the first mixing is obtained Material；
(2) activation of leavening: compound bacteria A and compound bacteria B are added into activation medium respectively, must be fermented after culture activation Liquid A and fermentation liquid B；
(3) it ferments: step (2) is added toward step (1) resulting first mixture and prepare resulting fermentation liquid A, obtain second Mixture, aerobic fermentation are added fermentation liquid B, obtain third mixture, sealed fermenting.
(4) low molecule fulvic acid is added toward step (3) resulting third mixture, stirs and evenly mixs, can must can be used for pond The complex microorganism preparations that pool ammonia nitrogen and dissolved oxygen are adjusted.
In an embodiment of the present invention, in the step (2), activation medium is comprised the following components in parts by weight: cultivation 1000 parts of pond water, 20 parts of brown sugar, 0.5 part of ammonium sulfate, 0.05 part of epsom salt, 0.5 part of dipotassium hydrogen phosphate, calcium carbonate 14.29 parts, 2 parts of sodium chloride, 1 part of brine, 0.02 part of a water calcium chloride, 0.05 part of ferrous sulfate heptahydrate, cupric sulfate pentahydrate 0.05 Part.
In an embodiment of the present invention, in the step (2), the activation culture temperature of compound bacteria A is 27-30 DEG C, culture Time is 24-48 hours, is activated by the way of having oxygen activation.
In an embodiment of the present invention, in the step (2), the activation culture temperature of compound bacteria B is 27-30 DEG C, culture Time is 24-48 hours, is activated by the way of sealing activation.
In an embodiment of the present invention, in the step (3), the weight proportion of the first mixture and fermentation liquid A are 1:2.
In an embodiment of the present invention, in the step (3), the time of the second mixture aerobic fermentation is 2-3 days.
In an embodiment of the present invention, in the step (3), the time of third mixture sealed fermenting is 5-7 days.
In an embodiment of the present invention, in the step (4), the additional amount of low molecule fulvic acid is the 1 of third mixture ~2%.
Fourth aspect present invention provide as described in the first aspect of the invention can be used for that pond ammonia nitrogen and dissolved oxygen adjust answer Leavening, the composite microbial that can be used for pond ammonia nitrogen and dissolved oxygen and adjust as described in respect of the second aspect of the invention for closing microorganism formulation Object preparation or the preparation as described in the third aspect of the present invention that can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted Application of the method in aquaculture water quality regulation and control field.
The present invention has the beneficial effect that
One, by reasonably combined various probiotics, using all kinds of fermentation raw materials, and meanwhile it is living by some biologies of fermentation generation Sex factor can be used as effective carbon source in environment, and the probiotics in directive breeding environment can fully demonstrate hair used Synergistic effect between yeast-like fungi kind, the reduction ammonia nitrogen harm of highly effective and safe, improves water body safety guarantee, improves the various physics and chemistry of water body Index improves water quality and substrate.
Two, by algae-bacteria symbiotic system, amino acid, the dimension of the biological organic matter rich in easily absorbed and balance are introduced A large amount of active materials such as raw element, microelement and a large amount of beneficial viable bacterias, various nutrition are complete, and proportion rationally, is applied in aquaculture Breeding water body active organic matter content can be obviously increased after, improves the various physical and chemical indexes of water body, improve water quality and substrate, Balance water body algae phase and bacterium phase.
Three, whole simple process, the requirement to equipment is not also high, strong operability, easily in scale raising or It is to be promoted the use of in aquaculture cooperative society.
Specific embodiment
The following is a preferred embodiment of the present invention, it is noted that for those skilled in the art For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as Protection scope of the present invention.
Below by a preferred embodiment of the present invention will be described in detail.The experiment of actual conditions is not specified in embodiment Method, usually according to normal condition.In the embodiment of the present invention unless otherwise noted, agents useful for same and consumptive material are commercial goods.
Embodiment 1
1, the preparation of leavening
1) preparation of activation medium
Leavening activation activation medium used comprises the following components in parts by weight: 1000 parts of cultivating pool water, brown sugar 20 parts, 0.5 part of ammonium sulfate, 0.05 part of epsom salt, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, halogen 1 part of water, 0.02 part of a water calcium chloride, 0.05 part of ferrous sulfate heptahydrate, 0.05 part of cupric sulfate pentahydrate.
2) compound bacteria A is activated:
1) addition prepares resulting activation medium by the way of having oxygen activation, 30 DEG C of cultures are activated for 36 hours, Obtain fermentation liquid A.
3) activation of compound bacteria B:
1) addition prepares resulting activation medium by the way of sealing activation, 30 DEG C of cultures are activated for 36 hours, Obtain fermentation liquid B.
2, it can be used for the preparation for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted
1) pretreatment of fermentation raw material
Dregs of beans and wheat bran that weight proportion is 1:1 are crushed, the first mixture is obtained；
2) it ferments
The fermentation liquid A of 2 times of quality is added toward resulting first mixture of step 1), stirs evenly, obtains the second mixture, 30 DEG C, aerobic fermentation 3 days, be then added with the fermentation liquid B of fermentation liquid equivalent, obtain third mixture, sealed fermenting 7 days.
3) 1.5% low molecule fulvic acid is added, stirs and evenly mixs, is in Umber pulp-water shape organic liquid, under have fermentation heavy It forms sediment, stirring is in suspension, there is fermenting aroma, as can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted.Gained Biological agent use when with pool water dilute 10 times, full pool spilling head.
Embodiment 2
1, the preparation of leavening
1) preparation of activation medium
Leavening activation activation medium used comprises the following components in parts by weight: 1000 parts of cultivating pool water, brown sugar 20 parts, 0.5 part of ammonium sulfate, 0.05 part of epsom salt, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, halogen 1 part of water, 0.02 part of a water calcium chloride, 0.05 part of ferrous sulfate heptahydrate, 0.05 part of cupric sulfate pentahydrate.
2) compound bacteria A is activated:
1) addition prepares resulting activation medium by the way of having oxygen activation, 27 DEG C of cultures are activated for 48 hours, Obtain fermentation liquid A.
3) activation of compound bacteria B: 1) addition prepares resulting activation medium by the way of sealing activation, 27 DEG C of cultures It is activated within 48 hours, obtains fermentation liquid B.
2, it can be used for the preparation for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted
1) pretreatment of fermentation raw material
Dregs of beans and wheat bran that weight proportion is 1:1 are crushed, the first mixture is obtained；
2) it ferments
The fermentation liquid A of 2 times of quality is added toward resulting first mixture of step 1), stirs evenly, obtains the second mixture, 30 DEG C, aerobic fermentation 3 days, be then added with the fermentation liquid B of fermentation liquid equivalent, obtain third mixture, sealed fermenting 7 days.
3) be added 2% low molecule fulvic acid, stir and evenly mix, be in Umber pulp-water shape organic liquid, under have fermentation precipitate, Stirring is in suspension, there is fermenting aroma, as can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted.Resulting life 15 times are diluted with the pool water when object preparation uses, full pool spilling head.
Embodiment 3
1, the preparation of leavening
1) preparation of activation medium
Leavening activation activation medium used comprises the following components in parts by weight: 1000 parts of cultivating pool water, brown sugar 20 parts, 0.5 part of ammonium sulfate, 0.05 part of epsom salt, 0.5 part of dipotassium hydrogen phosphate, 14.29 parts of calcium carbonate, 2 parts of sodium chloride, halogen 1 part of water, 0.02 part of a water calcium chloride, 0.05 part of ferrous sulfate heptahydrate, 0.05 part of cupric sulfate pentahydrate.
2) compound bacteria A is activated:
1) addition prepares resulting activation medium by the way of having oxygen activation, 30 DEG C of cultures are activated for 24 hours, Obtain fermentation liquid A.
3) activation of compound bacteria B:
1) addition prepares resulting activation medium by the way of sealing activation, 30 DEG C of cultures are activated for 24 hours, Obtain fermentation liquid B.
2, it can be used for the preparation for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted
1) pretreatment of fermentation raw material
The dregs of beans and wheat bran for being 1:1 by weight proportion) it crushes, obtain the first mixture；
2) it ferments
The fermentation liquid A of 4 times of quality is added toward resulting first mixture of step 1), stirs evenly, obtains the second mixture, 30 DEG C, aerobic fermentation 2 days, be then added with the fermentation liquid B of fermentation liquid equivalent, obtain third mixture, sealed fermenting 5 days.
3) be added 1% low molecule fulvic acid, stir and evenly mix, be in Umber pulp-water shape organic liquid, under have fermentation precipitate, Stirring is in suspension, there is fermenting aroma, as can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted.Resulting life 20 times are diluted with the pool water when object preparation uses, full pool spilling head.
Application Example
Application test of the complex microorganism preparations provided by the invention in raw fish culture
1, test products: the biological agent of embodiment 1, total viable count is in 1,000,000,000/milliliter or more.
2, raw fish: stocking size 5-6cm is tested, per acre about 5000 tail.If 2 experiment pools.
3, test site: fish culture field is given birth in Shunde District, Fushan City, Guangdong Province.
4, test method: when daily raising, 1 biological agent of Example closes pond and uniformly splashes.It uses once, often within every five days Mu breeding water body dosage is 1~2L, when water quality deterioration double using.And control group does not use biological agent.
5, test result:
The using effect for the biological agent that embodiment 1 provides, which is mainly reflected in, to be improved water quality, adjusts pond ammonia nitrogen and dissolved oxygen With controlling disease etc., specifically:
1) improve water quality, when water quality aging, complex microorganism preparations of splashing, visible water colour is salubrious, tender, living within second day, During use, water colour is preferable, and pH stable, algal grown is vigorous, has no down that algae etc. occurs；
2) complex microorganism preparations periodically provided using embodiment 1, pond ammonia nitrogen maintain reduced levels, breeding process Substantially disinfectant, the whole not used any antibiotic of cultivation are not used；
3) cultivation is whole, and 2 raw fishes of experiment pool of the biological agent provided using embodiment 1 have no disease substantially, The raw fish of two two jin of ponds or more specification has reached most probably half, averagely procurement price 14 yuan of fish per jin, finally two mouthfuls of lifes through calculating Proceed 2,000,000 in fishpond.
The above embodiments are merely illustrative of the technical solutions of the present invention rather than limiting the scope of the invention, although ginseng The present invention is explained in detail according to preferred embodiment, those skilled in the art should understand that, it can be to of the invention Technical solution is modified or replaced equivalently, without departing from the spirit and scope of technical solution of the present invention.

Claims (10)

1. a kind of leavening that can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted, which is characterized in that including multiple Combined bacteria A and compound bacteria B；Wherein, the compound bacteria A includes one of nitrobacteria, bacillus or a variety of；The compound bacteria B includes one of lactic acid bacteria, yeast or a variety of.
2. leavening as described in claim 1, which is characterized in that the percentage that the nitrobacteria content accounts for total bacteria count is 10%~30%；The percentage that the bacillus content accounts for total bacteria count is 30%~50%；The content of lactic acid bacteria accounts for total bacterium Several percentage is 20%~40%；The percentage that the amount of yeast accounts for total bacteria count is 5%~15%.
3. a kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust, which is characterized in that mainly wanted by such as right The activated post-fermentation raw material preparation gained of leavening described in asking 1, wherein the raw material includes the dregs of beans that weight proportion is 1:1 And wheat bran.
4. biological agent as claimed in claim 3, which is characterized in that leavening activation activation medium packet used Include the component of following parts by weight: 800-1200 parts of cultivating pool water, 10-30 parts of brown sugar, 0.1-1 parts of ammonium sulfate, epsom salt 0.01-0.1 parts, 0.2-0.8 parts of dipotassium hydrogen phosphate, 10-20 parts of calcium carbonate, 1-3 parts of sodium chloride, 0.5-2 parts of brine, a water chlorination 0.01-0.05 parts of calcium, 0.01-0.1 parts of ferrous sulfate heptahydrate, 0.01-0.1 parts of cupric sulfate pentahydrate.
5. a kind of preparation method that can be used for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted, which is characterized in that including Following steps:
(1) pretreatment of fermentation raw material: dregs of beans, wheat bran that weight ratio is 1:1 are crushed, the first mixture is obtained；
(2) activation of leavening: compound bacteria A and compound bacteria B are added into activation medium respectively, obtain fermentation liquid A after culture activation And fermentation liquid B；
(3) it ferments: step (2) is added toward step (1) resulting first mixture and prepare resulting fermentation liquid A, obtain the second mixing Material, aerobic fermentation are added fermentation liquid B, obtain third mixture, sealed fermenting；
(4) low molecule fulvic acid is added toward step (3) resulting third mixture, stirs and evenly mixs, can must can be used for pond ammonia The complex microorganism preparations that nitrogen and dissolved oxygen are adjusted.
6. preparation method as claimed in claim 5, which is characterized in that in the step (2), the activation culture temperature of compound bacteria A Degree is 27-30 DEG C, and incubation time is 24-48 hours, is activated by the way of having oxygen activation.
7. preparation method as claimed in claim 5, which is characterized in that in the step (2), the activation culture temperature of compound bacteria B Degree is 27-30 DEG C, and incubation time is 24-48 hours, is activated by the way of sealing activation.
8. preparation method as claimed in claim 5, which is characterized in that in the step (3), the first mixture, fermentation liquid A and The weight proportion of fermentation liquid B is 1:2:2.
9. preparation method as claimed in claim 5, which is characterized in that in the step (3), the second mixture aerobic fermentation Time is 2-3 days, and the time of third mixture sealed fermenting is 5-7 days.
10. can be used for the leavening for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted as described in claim 1, such as weighing Benefit can be used for the complex microorganism preparations or such as claimed in claim 5 may be used that pond ammonia nitrogen and dissolved oxygen are adjusted described in requiring 3 Preparation method answering in aquaculture water quality regulation and control field for the complex microorganism preparations that pond ammonia nitrogen and dissolved oxygen are adjusted With.
CN201810950317.XA 2018-08-20 2018-08-20 A kind of complex microorganism preparations that can be used for pond ammonia nitrogen and dissolved oxygen and adjust Pending CN109082394A (en)

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Application publication date: 20181225