CN109072203A - 镜像核酸复制体系 - Google Patents

镜像核酸复制体系 Download PDF

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Publication number
CN109072203A
CN109072203A CN201780026142.7A CN201780026142A CN109072203A CN 109072203 A CN109072203 A CN 109072203A CN 201780026142 A CN201780026142 A CN 201780026142A CN 109072203 A CN109072203 A CN 109072203A
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nucleic acid
polymerase
dna
mirror
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CN109072203B (zh
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朱听
刘磊
徐维亮
王子谋
姜文君
王佳星
俞林萍
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Tsinghua University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07048—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

提供了对镜像核酸进行复制的方法，包括：在镜像核酸聚合酶、镜像核酸模板、镜像核酸引物以及镜像dNTPs/rNTPs的存在下进行反应，以获得所述镜像核酸。

Description

PCT国内申请，说明书已公开。

Claims (28)

  1. PCT国内申请，权利要求书已公开。
CN201780026142.7A 2015-08-18 2017-02-15 镜像核酸复制体系 Active CN109072203B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201510507780.3A CN106467910A (zh) 2015-08-18 2015-08-18 L-dna/l-rna聚合酶及其应用
CNPCT/CN2016/079006 2016-04-11
PCT/CN2016/079006 WO2017028548A1 (zh) 2015-08-18 2016-04-11 镜像核酸复制体系
PCT/CN2017/073573 WO2017177759A1 (zh) 2015-08-18 2017-02-15 镜像核酸复制体系

Publications (2)

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CN109072203A true CN109072203A (zh) 2018-12-21
CN109072203B CN109072203B (zh) 2023-04-04

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CN201510507780.3A Pending CN106467910A (zh) 2015-08-18 2015-08-18 L-dna/l-rna聚合酶及其应用
CN201780026142.7A Active CN109072203B (zh) 2015-08-18 2017-02-15 镜像核酸复制体系

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US (2) US11371027B2 (zh)
EP (1) EP3444341A4 (zh)
CN (2) CN106467910A (zh)
WO (2) WO2017028548A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114891868A (zh) * 2022-05-31 2022-08-12 广州市金圻睿生物科技有限责任公司 一种基于ngs平台的微生物定量方法及试剂盒

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106467910A (zh) 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
CA3094598A1 (en) * 2018-04-05 2019-10-10 Tsinghua University Methods of sequencing and producing nucleic acid sequences
CN109022387B (zh) * 2018-08-29 2020-12-22 华南理工大学 一种突变型Pfu DNA聚合酶及其制备方法和应用
CN111217892A (zh) * 2019-07-26 2020-06-02 翔宇药业股份有限公司 卡贝缩宫素的合成方法
WO2023148646A1 (en) * 2022-02-03 2023-08-10 Tsinghua University Mirror-image selection of l-nucleic acid aptamers

Citations (3)

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Publication number Priority date Publication date Assignee Title
US20110229976A1 (en) * 2008-10-29 2011-09-22 Noxxon Pharma Ag Sequencing of nucleic acid molecules by mass spectrometry
CN104379748A (zh) * 2012-05-16 2015-02-25 诺松制药股份公司 L-核酸的酶促合成
WO2015074756A1 (en) * 2013-11-20 2015-05-28 Noxxon Pharma Ag Synthesis of l-nucleic acids by means of an enzyme

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JP5616584B2 (ja) * 2009-01-09 2014-10-29 富士フイルム株式会社 核酸配列の複製方法
JP6138684B2 (ja) * 2010-08-31 2017-05-31 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． マイクロ流体デバイスのインシステムプライミングのための組成物及び方法
CN102517241B (zh) * 2011-12-19 2013-06-19 中国药科大学 一种含有d型氨基酸的双羰基还原酶的突变体的制备方法
CN106467910A (zh) 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110229976A1 (en) * 2008-10-29 2011-09-22 Noxxon Pharma Ag Sequencing of nucleic acid molecules by mass spectrometry
CN104379748A (zh) * 2012-05-16 2015-02-25 诺松制药股份公司 L-核酸的酶促合成
WO2015074756A1 (en) * 2013-11-20 2015-05-28 Noxxon Pharma Ag Synthesis of l-nucleic acids by means of an enzyme

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GREGORY A. GRANT: "《Synthetic Peptides A User"s Guide》", 31 December 2002, OXFORD UNIVERSITY PRESS *
SUSANNE LEVA等: "GnRH binding RNA and DNA Spiegelmers: a novel approach toward GnRH antagonism", 《CHEMISTRY & BIOLOGY》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114891868A (zh) * 2022-05-31 2022-08-12 广州市金圻睿生物科技有限责任公司 一种基于ngs平台的微生物定量方法及试剂盒

Also Published As

Publication number Publication date
WO2017028548A1 (zh) 2017-02-23
EP3444341A4 (en) 2019-09-04
EP3444341A1 (en) 2019-02-20
WO2017177759A1 (zh) 2017-10-19
US20190376050A1 (en) 2019-12-12
US20220325260A1 (en) 2022-10-13
US11371027B2 (en) 2022-06-28
CN109072203B (zh) 2023-04-04
CN106467910A (zh) 2017-03-01

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