CN109043232A - A kind of raising snakehead survival rate and the feed of anti-stress ability and preparation method thereof - Google Patents
A kind of raising snakehead survival rate and the feed of anti-stress ability and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of raising snakehead survival rate and the feeds of anti-stress ability, including following components: krill meal, fish meal, dehulled soybean meal, chicken meal, brewer's yeast, peanut press pulp, flour, fish oil, soya-bean oil, soybean lecithin, multi-vitamins, composite mineral salt, calcium dihydrogen phosphate, concentration dephenolization cottonseed protein, vitamin C phosphoric ester, choline chloride, haematococcus pluvialis, DL- METHIONINE, lysine hydrochloride, threonine and taurine.A kind of new Cottonseed Meal protein sources-concentration dephenolization cottonseed protein is added in feed of the invention, its main feature is that protein content is close with fish meal, without containing excessive anti-nutritional factors, after amino acid balance amino acid ratio is added, it can achieve the purpose that reduce fish meal usage amount, not will cause the growth retardation of snakehead, the effect for also acting as and saving fish meal resource, reduce feed cost, discharge of the snakehead for nitrogen phosphorus is reduced simultaneously, the eutrophication of anti-water-stop body is a kind of environment-friendly feed.
Description
Technical field
The invention belongs to aquatic feeds technical fields, and in particular, to a kind of to reduce fish using concentration dephenolization cottonseed protein
Powder dosage simultaneously adds environment-friendly type mixed feed and its preparation that haematococcus pluvialis improves snakehead cultivation survival rate and anti-stress ability
Method.
Background technique
Snakehead is commonly called as snakeheaded fish, raw fish, and natural disposition is violent, and reproductive capacity is strong, is a kind of meat that cultivation range is very wide in China
Property fish.Its delicious meat, the speed of growth is fast, and premunition is strong, strong to the tolerance of severe aquatic environment, since the success of its fry
The popularization cultivation of large area is obtained after cultivating, it has also become the important freshwater aquiculture product of the one kind in China or even Southeast Asia.Make
Optimum dietary protein level for predacious fish, murrel flying fish is usually relatively high, in order to meet higher protein requirement, in feed
Fish meal can be largely used, causes the raising of feed cost, while the high fish meal in feed will increase aquatic animal and be discharged into water body
In ammonia nitrogen and phosphorus content, the alga eruption for easily causing water body environment causes water eutrophication, has to ecological environment
Undesirable influence.
Current snakehead cultural technique is higher and higher, and the snakehead output demand of unit of water body area is also gradually increased, in pearl
Triangular portions cultivation area, single per mu yield of snakehead have been increased to 40000 pounds/acre from 10000 pounds/acre.In such high density
In the case of cultivation, the stress of various environmental factors (temperature change, precipitation lead to salinity altercation, density etc.) is so that being immunized for snakehead
Ability and anti-stress ability require to improve, and the precipitation of especially Pearl River Delta Area during Summer is easy to lead to the salinity of breeding water body
Change dramatically, which occurs, influences the growth of snakehead, and is easy to generate dead and corresponding complication because of immunity deficiency.
Therefore the feed of a kind of immunocompetence that snakehead can be improved and anti-stress ability is urgently studied.
Summary of the invention
A kind of ring lower the object of the present invention is to provide fish meal content and that snakehead survival rate and anti-stress ability can be improved
Protect mixed feed.
To achieve the goals above, the present invention takes following scheme:
A kind of feed improving snakehead survival rate and anti-stress ability, the component including following weight percent: krill meal 1
~10%, fish meal 10~30%, dehulled soybean meal 15~40%, chicken meal 1~15%, brewer's yeast 1~10%, peanut press pulp 1~
15%, flour 10~20%, fish oil 1~10%, soya-bean oil 0.5~5%, soybean lecithin 1~8%, multi-vitamins 0.5~5%,
Dephenolization cottonseed protein 0~20%, vitamin C phosphoric ester 0.05 is concentrated in composite mineral salt 0.5~6%, calcium dihydrogen phosphate 1~8%
~5%, choline chloride 0.1~3%, haematococcus pluvialis 0~3%, DL- METHIONINE 0~4%, lysine hydrochloride 0~4%, Soviet Union
Propylhomoserin 0~3% and taurine 0~3%.
Preferably, the feed of snakehead survival rate and anti-stress ability, the component including following weight percent: krill are improved
Powder 3~7%, fish meal 15~25%, dehulled soybean meal 20~30%, chicken meal 5~10%, brewer's yeast 2~6%, peanut press pulp 3~
10%, flour 11~17%, fish oil 1~10%, soya-bean oil 1~3%, soybean lecithin 2~8%, multi-vitamins 0.5~3%, again
Conjunction mineral salt 0.5~4%, calcium dihydrogen phosphate 2~5%, concentration dephenolization cottonseed protein 0~8%, vitamin C phosphoric ester 0.1~
1%, choline chloride 0.1~1%, haematococcus pluvialis 0.1~1.5%, DL- METHIONINE 0.1~0.8%, lysine hydrochloride 0.1
~1%, threonine 0.05~0.6% and taurine 0.05~0.7%.
Preferably, the feed of snakehead survival rate and anti-stress ability, the component including following weight percent: krill are improved
Powder 5%, fish meal 22%, dehulled soybean meal 25%, chicken meal 7%, brewer's yeast 3%, peanut press pulp 6%, flour 13.84%, fish oil
2.43%, soya-bean oil 1%, soybean lecithin 1.5%, multi-vitamins 1%, composite mineral salt 1%, calcium dihydrogen phosphate 2.19% is dense
Contracting dephenolization cottonseed protein 8%, vitamin C phosphoric ester 0.1%, choline chloride 0.2%, haematococcus pluvialis 0.33%, DL- METHIONINE
0.08%, lysine hydrochloride 0.22%, threonine 0.06% and taurine 0.05%.
Preferably, the fish meal selects imported fish meal.
Preferably, every kilogram of multi-vitamins contain following component: 450-500mg thiamine, 1200-2000mg core
Flavine, 600-1200mg pyridoxal, 2500-3500mg niacinamide, 1200-2000mg calcium pantothenate, 1-6mg biotin, 50-
100mg folic acid, 3500-5000mg inositol, 2500-4000mg vitamin E, 350000-450000IU vitamin A, 2-8mg dimension life
Plain B12,200-300mg Vitamin K3,70000-90000IU vitamine D3 and 5000-6500mg vitamin C.
It is highly preferred that every kilogram of multi-vitamins contain following component: 480mg thiamine;1600mg riboflavin;
1000mg pyridoxal;3000mg niacinamide;1700mg calcium pantothenate;4mg biotin;70mg folic acid;4000mg inositol;3200mg dimension
Raw element E;400000IU vitamin A;4mg vitamin B12;250mg Vitamin K3;80000IU vitamine D3;6000mg vitamin
C。
Preferably, every kilogram of composite mineral salt contains following component: ZnSO4·H2O15000-17000mg、
MnSO4·H2O2400-2550mg、Ca(IO3)2350-400mg、FeSO4·H2O5000-5800mg、MgSO4·H2O17000-
18000mg、CuSO4·H2O900-1000mg、CoSO4·H2O100-200mg and NaSeO3·H2O 20-30mg。
It is highly preferred that every kilogram of composite mineral salt contains following component: ZnSO4·H2O16423mg、MnSO4·
H2O2456mg、Ca(IO3)2384mg、FeSO4·H2O5462mg、MgSO4·H2O17298mg、CuSO4·H2O973mg、
CoSO4·H2O160mg and NaSeO3·H2O26mg。
Cottonseed Meal is the byproduct of cottonseed oil expression industry, its Chinese yield only this be Chinese third in dregs of beans and rapeseed meal
Big oilseed extraction product, has the advantages that other plant protein sources, i.e. stable yield and cheap, is suitable as substituting
The protein sources of fish meal.But there is a big difference for the amino acid composition and ratio and fish meal in common Cottonseed Meal albuminoid source, and contains
A large amount of anti-nutritional factors limits its application in aquatic feeds.The high-quality protein of fish meal is in aquatic animal body simultaneously
The important source material of various antioxidases is synthesized, the previous immunity that frequently can lead to aquatic animal using vegetable protein substitution fish meal
The case where capable of declining.
Haematococcus pluvialis is a kind of highest microalgae of natural astaxanthin content, and astaxanthin is a Carotenoids, can be improved
The oxidation resistance and strengthen immunity of fish shrimp crab, while the survival rate and growth of cultivated animals in breeding process can be improved
Energy.Astaxanthin simultaneously in haematococcus pluvialis is most of based on trans-, phase consistent with the intracorporal astaxanthin form of aquatic animal
It is easier to be absorbed by animals compared with artificial synthesized astaxanthin.
A kind of preparation method for the feed improving snakehead survival rate and anti-stress ability, comprising the following steps:
(1) each component raw material is weighed according to the proportion, and by krill meal therein, fish meal, dehulled soybean meal, chicken meal, peanut
Bran and flour grinding and sieving;
(2) by krill meal, fish meal, dehulled soybean meal, chicken meal, peanut press pulp, flour and the chlorination gallbladder after sieving in step (1)
Alkali, multi-vitamins, calcium dihydrogen phosphate and composite mineral salt are uniformly mixed, and material after mixing is pulverized and sieved again,
Obtain component raw material;
(3) component raw material after being sieved in step (2) and concentration dephenolization cottonseed protein, vitamin C phosphoric ester, rain is raw red
Ball algae, soya-bean oil, fish oil, soybean lecithin, DL~methionine, lysine hydrochloride, threonine, brewer's yeast and taurine mixing;
(4) by mixed material in step (3) be passed through in modem anhydrous steam modulation, then by material into
Row granulation;
(5) particle obtained is dried, then the feed for improving snakehead survival rate and anti-stress ability is made.
Preferably, in the step (1) and (2), crushing is carried out in pulverizer, the mesh number that when sieving uses is 90~
120 mesh.
Preferably, in the step (3), mixing to the uniformity is 95%~100%.
Preferably, in the step (4), the temperature of anhydrous steam modulation is the time of 95~110 DEG C and modulation are as follows: 8~
10 minutes.
Preferably, in the step (4), 100~120 DEG C at a temperature of will material put into extrusion granulator in make
Grain;
Preferably, in the step (5), particle obtained is dried at a temperature of 80~95 DEG C.
The beneficial effects of the present invention are:
1, a kind of new Cottonseed Meal protein sources-concentration dephenolization cottonseed protein is taken, its main feature is that protein content and fish
Powder is close, and does not contain excessive anti-nutritional factors.
2, after amino acid (threonine, methionine, lysine and taurine) amino acid ratio being added, reduction can be reached
The purpose of fish meal usage amount, and not will cause the growth retardation of snakehead, oxidation resistance is unaffected.It plays and saves fish
Powder resource, the effect for reducing feed cost, while reducing discharge of the snakehead for nitrogen phosphorus, the eutrophication of anti-water-stop body.
3, haematococcus pluvialis is added on the basis of low fish-meal to further increase surviving for snakehead in breeding process
Rate and for stress tolerance.
Specific embodiment
The present invention is made below in conjunction with specific embodiment and further being elaborated, the embodiment is served only for explaining this
Invention, is not intended to limit the scope of the present invention.Test method as used in the following examples is normal unless otherwise specified
Rule method;Used material, reagent etc., unless otherwise specified, for the reagent and material commercially obtained.
The present embodiment is to be concentrated dephenolization cottonseed protein as the protein sources of substitution fish meal.It is added after obtaining most suitable substitution amount
Haematococcus pluvialis compares two gradient haematococcus pluvialis to snakehead growth performance and immune influence.
It is tested according to the design formula feed of table 1.Wherein embodiment 1 is control group, and 2~embodiment of embodiment 6 is
Fish meal alternate sets, test result are compared to Evaluated effect with alternate sets and control group respectively.7~embodiment of embodiment 8 is
Haematococcus pluvialis group, test result is respectively compared with haematococcus pluvialis group and embodiment 3 carrys out Evaluated effect.
Test index described in each embodiment includes: rate of body weight gain WG (%), specific growth rate SGR (%d~1), feed coefficient
(FCR), survival rate SR (%), protein efficiency ratio, PER (PER).Index calculation formula is as follows:
Specific growth rate SGR (%d~1)=100 × (the ln terminal counterpoise~initial counterpoise of ln)/experiment number of days
The first counterpoise in rate of body weight gain WG (%)=(terminal counterpoise~initial counterpoise) × 100/
Sheng Xia mantissa × 100/ experiment starts Shi Fang shrimp mantissa at the end of survival rate SR (%)=experiment
Feed coefficient (FCR)=feedstuff feeding amount/(terminal weight~original body mass)
The full fish body composition and liver anti-oxidant relevant immune indexes SOD, CAT, T of each embodiment snakehead are measured simultaneously
~AOC and lipid peroxidation product MDA content.
1 feed embodiment of table and Analysis of Nutritive Composition (%)
In the multi-vitamins that embodiment uses, every kg multi-vitamins contain following component: thiamine 480mg;Core yellow
Plain 1600mg;Pyridoxal 1000mg;Niacinamide 3000mg;Calcium pantothenate 1700mg;Biotin 4mg;Folic acid 70mg;Inositol
4000mg;3 200mg of vitamin E;Vitamin A 400000IU;Vitamin B12 4mg;Vitamin K3 250mg;Vitamine D3
80000IU;Vitamin C 6000mg.
In the composite mineral salt that embodiment uses, every kg composite mineral salt contains following component: ZnSO4
H2O16243mg;MnSO4·H2O2458mg;Ca(IO3)2384mg;FeSO4·H2O5462mg;MgSO4·H2O17298mg;
CuSO4·H2O973mg;CoSO4·H2O160mg;NaSeO3·H2O26mg。
The feeding for improving snakehead survival rate and anti-stress ability is prepared in the raw material of above-described embodiment 1-8 in following manner
Material:
A kind of preparation method for the feed improving snakehead survival rate and anti-stress ability, comprising the following steps:
(1) each component raw material is weighed according to the proportion, and by krill meal therein, fish meal, dehulled soybean meal, chicken meal, peanut
Bran and flour grinding and sieving;
(2) by krill meal, fish meal, dehulled soybean meal, chicken meal, peanut press pulp, flour and the chlorination gallbladder after sieving in step (1)
Alkali, multi-vitamins, calcium dihydrogen phosphate and composite mineral salt are uniformly mixed, and material after mixing is pulverized and sieved again,
Obtain component raw material;
(3) component raw material after being sieved in step (2) and concentration dephenolization cottonseed protein, vitamin C phosphoric ester, rain is raw red
Ball algae, soya-bean oil, fish oil, soybean lecithin, DL- METHIONINE, lysine hydrochloride, threonine, brewer's yeast and taurine are sufficiently mixed
It closes uniformly, mixture homogeneity 98%;
(4) material in step (3) after mixing is passed through in modem 100 DEG C of anhydrous steams and modulates 10 points
Clock, then make material 120 DEG C at a temperature of enter extrusion granulator in pelletize;
(5) it at a temperature of 95 DEG C, by particle obtained drying, cooling, is made and improves snakehead survival rate and anti-stress ability
Feed.
Influence of 2 mixed feed of table to snakehead growth performance
1. Examples 1 to 6 is that the embodiment of dephenolization cottonseed protein is concentrated, comparative example 1, embodiment 2 can see
With embodiment 3, it is seen that survival rate, specific growth rate, growth rate and feed coefficient are not significantly different (P > 0.05), illustrate to raise
Fish meal has no adverse effect the growth of snakehead after reducing in material, that is, thinks with 8% concentration dephenolization cottonseed protein substitution fish meal not
It will affect the growth of snakehead.
2. the haematococcus pluvialis embodiment 7~8 of two kinds of gradients of addition is compared with Example 3 significantly increased to weight gain, but
0.66% does not increase significantly relative to 0.33% group.Two groups of survival rate has reached 100%.In view of the raw red ball of rain
The cost of algae, 0.33% additive amount can meet production requirement.
Influence (part) of 3 mixed feed of table to the full nutrition components of snakehead
Pass through the full nutrition components result of comparative example 1, embodiment 2 and embodiment 3, it can be seen that utilize concentration dephenolize
Cottonseed protein has no significant effect the crude protein of the full fish of snakehead after reducing the fish meal in feed, illustrates that the protein sources can be as having
The protein sources of the substitution fish meal of effect.Comparative example 3 and embodiment 7, the full fish body of embodiment 8 form result, it can be seen that rain is raw
Haematococcus does not influence the body composition of fish.
Influence of the low fish-meal of table 4 to snakehead liver pancreas Antioxidant Indexes
The low fish meal Snakeheaded fish feeding stuff is assessed by the Antioxidant Indexes in measurement snakehead liver pancreas to cultivation snakehead
The influence of oxidation resistance.
Known to comparative example 1 and embodiment 2, the result of embodiment 3: feeding snakehead with the low fish-meal will not drop
The activity of oxidation resistant enzyme, shows that its antioxygenic property is not affected in its low liver pancreas.Comparative example 3 and embodiment
7, the oxidation resistance of snakehead liver can be significantly improved by adding haematococcus pluvialis known to embodiment 8 in feed, and can be significant
Reduce the generation of lipid peroxidation product MDA in liver.0.66% haematococcus pluvialis and 0.33% haematococcus pluvialis compare nothing
The advantage of conspicuousness, therefore the appropriate haematococcus pluvialis of addition can significantly improve the oxidation resistance of snakehead liver, rather than
It is more, it significantly improves bigger.The raising of snakehead activities of antioxidant enzymes can promote its reply stress when ability.
Claims (10)
1. a kind of feed for improving snakehead survival rate and anti-stress ability, which is characterized in that the group including following weight percent
Point: krill meal 1~10%, fish meal 10~30%, dehulled soybean meal 15~40%, chicken meal 1~15%, brewer's yeast 1~10%,
Peanut press pulp 1~15%, flour 10~20%, fish oil 1~10%, soya-bean oil 0.5~5%, soybean lecithin 1~8%, multi-vitamins
0.5~5%, dephenolization cottonseed protein 0~20%, vitamin C is concentrated in composite mineral salt 0.5~6%, calcium dihydrogen phosphate 1~8%
Phosphoesterase 30 .05~5%, choline chloride 0.1~3%, haematococcus pluvialis 0~3%, DL- METHIONINE 0~4%, lysine hydrochloric acid
Salt 0~4%, threonine 0~3% and taurine 0~3%.
2. the feed according to claim 1 for improving snakehead survival rate and anti-stress ability, which is characterized in that including following
The component of weight percent: krill meal 3~7%, fish meal 15~25%, dehulled soybean meal 20~30%, chicken meal 5~10%, beer
Brewer yeast 2~6%, peanut press pulp 3~10%, flour 11~17%, fish oil 1~10%, soya-bean oil 1~3%, soybean lecithin 2~
8%, multi-vitamins 0.5~3%, composite mineral salt 0.5~4%, calcium dihydrogen phosphate 2~5%, concentration dephenolization cottonseed protein 0
~8%, vitamin C phosphoric ester 0.1~1%, choline chloride 0.1~1%, haematococcus pluvialis 0.1~1.5%, DL- METHIONINE
0.1~0.8%, lysine hydrochloride 0.1~1%, threonine 0.05~0.6% and taurine 0.05~0.7%.
3. the feed according to claim 1 for improving snakehead survival rate and anti-stress ability, which is characterized in that including following
The component of weight percent: krill meal 5%, fish meal 22%, dehulled soybean meal 25%, chicken meal 7%, brewer's yeast 3%, peanut press pulp
6%, flour 13.84%, fish oil 2.43%, soya-bean oil 1%, soybean lecithin 1.5%, multi-vitamins 1%, composite mineral salt 1%,
Calcium dihydrogen phosphate 2.19%, concentration dephenolization cottonseed protein 8%, vitamin C phosphoric ester 0.1%, choline chloride 0.2%, rain life are red
Ball algae 0.33%, DL- METHIONINE 0.08%, lysine hydrochloride 0.22%, threonine 0.06% and taurine 0.05%.
4. the feed according to any one of claim 1-3 for improving snakehead survival rate and anti-stress ability, feature exist
In every kilogram of multi-vitamins contain following component: 450-500mg thiamine, 1200-2000mg riboflavin, 600-
1200mg pyridoxal, 2500-3500mg niacinamide, 1200-2000mg calcium pantothenate, 1-6mg biotin, 50-100mg folic acid,
3500-5000mg inositol, 2500-4000mg vitamin E, 350000-450000IU vitamin A, 2-8mg vitamin B12,200-
300mg Vitamin K3,70000-90000IU vitamine D3 and 5000-6500mg vitamin C.
5. the feed according to any one of claim 1-3 for improving snakehead survival rate and anti-stress ability, feature exist
In every kilogram of composite mineral salt contains following component: ZnSO4·H2O15000-17000mg、MnSO4·H2O2400-
2550mg、Ca(IO3)2350-400mg、FeSO4·H2O5000-5800mg、MgSO4·H2O17000-18000mg、CuSO4·
H2O900-1000mg、CoSO4·H2O100-200mg and NaSeO3·H2O20-30mg。
6. the preparation method of described in any item feeds for improving snakehead survival rate and anti-stress ability according to claim 1~5,
Characterized by comprising the following steps:
(1) weigh each component raw material according to the proportion, and by krill meal therein, fish meal, dehulled soybean meal, chicken meal, peanut press pulp and
Flour grinding and sieving;
(2) by after sieving in step (1) krill meal, fish meal, dehulled soybean meal, chicken meal, peanut press pulp, flour and choline chloride,
Multi-vitamins, calcium dihydrogen phosphate and composite mineral salt are uniformly mixed, and material after mixing is pulverized and sieved again, is obtained
Component raw material;
(3) by the component raw material and the raw red ball of concentration dephenolization cottonseed protein, vitamin C phosphoric ester, rain after sieving in step (2)
Algae, soya-bean oil, fish oil, soybean lecithin, DL- METHIONINE, lysine hydrochloride, threonine, brewer's yeast and taurine mixing;
(4) mixed material in step (3) is passed through anhydrous steam modulation in modem, then by material system
Grain;
(5) particle obtained is dried, then the feed for improving snakehead survival rate and anti-stress ability is made.
7. preparation method according to claim 6, which is characterized in that in the step (1) and (2), crushing is to crush
It is carried out in machine, the mesh number that when sieving uses is 90~120 mesh.
8. preparation method according to claim 6, which is characterized in that in the step (3), mixing to the uniformity is 95%
~100%.
9. preparation method according to claim 6, which is characterized in that in the step (4), the temperature of anhydrous steam modulation
For 95~110 DEG C and modulation time are as follows: 8~10 minutes.
10. preparation method according to claim 6, which is characterized in that in the step (4), in 100~120 DEG C of temperature
Material is put into extrusion granulator under degree and is pelletized;In step (5), particle obtained is dried at a temperature of 80~95 DEG C
It is dry.
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Cited By (2)
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CN110810674A (en) * | 2019-12-20 | 2020-02-21 | 广州观星农业科技有限公司 | Raw fish compound feed and preparation method thereof |
CN111436546A (en) * | 2020-04-20 | 2020-07-24 | 广东康达尔农牧科技有限公司 | Raw fish compound feed, preparation method and application thereof |
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CN105614159A (en) * | 2015-12-25 | 2016-06-01 | 湖州海皇生物科技有限公司 | Puffed mixed feed for local ophicephalus argus in whole process |
CN107467415A (en) * | 2017-08-15 | 2017-12-15 | 兰溪市酉泽饲料技术服务有限公司 | The preparation method of environmental type shallow sea carp compound feed |
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CN104522416A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for juvenile fish of bostrichthys sinensis and preparation method of granular feed |
CN105614159A (en) * | 2015-12-25 | 2016-06-01 | 湖州海皇生物科技有限公司 | Puffed mixed feed for local ophicephalus argus in whole process |
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