CN109042291A - A method of novel vegetable is created using Chinese cabbage and wild cabbage distant hybridization - Google Patents

A method of novel vegetable is created using Chinese cabbage and wild cabbage distant hybridization Download PDF

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Publication number
CN109042291A
CN109042291A CN201810849131.5A CN201810849131A CN109042291A CN 109042291 A CN109042291 A CN 109042291A CN 201810849131 A CN201810849131 A CN 201810849131A CN 109042291 A CN109042291 A CN 109042291A
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China
Prior art keywords
cabbage
chinese cabbage
vegetable
seedling
wild
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CN201810849131.5A
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Chinese (zh)
Inventor
张大为
严明理
刘丽莉
周定港
程成
伍小方
高国应
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Hunan University of Science and Technology
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Hunan University of Science and Technology
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Priority to CN201810849131.5A priority Critical patent/CN109042291A/en
Publication of CN109042291A publication Critical patent/CN109042291A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses it is a kind of using Chinese cabbage and wild cabbage distant hybridization creation novel vegetable method, including step (1) choose vegetable with Chinese cabbage (B.rapa) and wild cabbage (B.oleracea) hybridization；(2) in pollination 15 days or so, silique is taken to carry out embryo rescue；(3) chromosome doubling is carried out to seedling using colchicine；(4) it is placed in field cultivation.The advantages that this method, which obtains distant hybrid progeny, has easily cultivation, and growth is vigorous, rich in nutrition content, can be used for creating novel vegetable kind.

Description

A method of novel vegetable is created using Chinese cabbage and wild cabbage distant hybridization
Technical field
The present invention relates to agriculture application fields, are especially to provide a kind of method for creating novel vegetable.
Background technique
Brassica genus (Brassica) be Cruciferae (Brassicaceae) the big category of trunk, including it is many have it is important The oil plant of economic value, feed, vegetable crop.
Vegetables in Brassica is rich in human bodies institutes such as dietary fiber, carrotene, vitamin C, minerals and glucosinolates Required nutritional ingredient, while having good inhibiting effect to anti-curing oncoma, cardiovascular disease, edible value is higher.Brassica genus vegetable Vegetables type is extremely abundant.Cabbage (B. rapa) vegetables include the mutation such as pakchoi, Chinese cabbage, Zicaitai, Wuta-tsai, cabbage heart. Wild cabbage class (B. oleracea) vegetable then includes cabbage mustard, cauliflower, broccoli, cabbage, violet cabbage, broccoli, spore Sub- wild cabbage etc..
Cabbage type rape (B. napus, AACC, 2n=38) by Chinese cabbage (B. rapa, AA, 2n=20) and wild cabbage (B. oleracea, CC, 2n=18) and hybridization chromosome doubling, the phase in terms of growth potential, setting percentage, disease-resistant, degeneration-resistant, adaptability There is inherent advantage than Chinese cabbage wild cabbage.However cabbage type rape is used as oil plant or feeding work more during long-term artificial selection Object utilizes, and rarely has the report as vegetables.
Therefore, using Chinese cabbage abundant and wild cabbage resource, there is the Wild cabbage type oil of vegetable value by distant hybridization creation Dish promotes the multi-functional utilization of rape, has most important theories and practice significance.
Summary of the invention
For above situation, this method can efficiently use existing Chinese cabbage and wild cabbage Vegetable Resources, create novel vegetable.
This method technical solution are as follows: a method of using Chinese cabbage and wild cabbage distant hybridization creation novel vegetable, including with Lower step
(1) choosing vegetable with Chinese cabbage and wild cabbage is parent, and Post flowering selects fine day to carry out hybridization pollination；
(2) in pollination 15 days or so, after silique is expanded, silique is taken to carry out disinfection, strips rataria and be put into MS culture medium and cultivates To seedling；
(3) when the seedling 3-4 leaf phase, move into the MS of colchicine containing 50mg/L culture medium in cultivate one week or so, to hybrid seedling into Row chromosome doubling；
(4) it doubles rear seedling to then move to without in colchicine MS culture medium, when the leaf phase to be grown to 4-5, transplanting is planted in field Training.
In the step (1) parent be vegetable with Chinese cabbage (B. rapa) and wild cabbage (B. oleracea) kind one or more Subspecies；
Culture medium can be added in colchicine by chromosome doubling in the step (3), directly processing test tube seedling.
Chromosome doubling in the step (3) can dig out plant in step (4), with 1g/L colchicine from soil Solution soaks root 6-8 hours, is planted again after flushing in field.
Beneficial effects of the present invention:
(1) Chinese cabbage and wild cabbage vegetable are more with resource, and parent draws materials convenient；
(2) Chinese cabbage and wild cabbage filial generation growth potential are vigorous, and yield and resistance are high；
(3) it is edible to can be used as novel vegetable for Chinese cabbage and wild cabbage filial generation, promotes the multi-functional utilization of rape；
(4) Chinese cabbage hybridizes to obtain novel cabbage type rape with wild cabbage, can widen its germplasm money with existing Brassica napus hybrid Source；
(5) multiple advantages such as this method has technical feasibility, easy to operate, and expense is low.
Specific embodiment
The present invention is described in detail With reference to embodiment.
Embodiment 1
It (1) is female parent with Chinese cabbage, cabbage mustard is male parent, and Post flowering selection fine day carries out taking the fresh anther pollination Chinese cabbage of cabbage mustard；
(2) in pollination 14-16 days, after silique is expanded, silique is taken to carry out disinfection, rataria is stripped on superclean bench and is put into MS training It supports and is cultured to be a seedling in base；
(3) it when seedling to the 3-4 leaf phase, moves into the MS of colchicine containing 50mg/L culture medium and cultivates one week or so；
(4) it doubles rear seedling to then move to without in colchicine MS culture medium, be expanded by tissue cultures numerous；
(5) it takes and doubles rear seedling leaves, flow cytometry has doubled；
(6) it when growth of seedling to the 4-5 leaf phase, transplants in field cultivation, plant forms do not have between parents' osculant, blade Show the balling characteristic of Chinese cabbage；
(7) it after plant buddings, takes ovary cell to carry out cytological observation and confirms that its chromosome number is 38, determination doubles success；
(8) Synthetic rapeseed stem tongue fur compares the obvious thickening of parents (30.23 ± 3.14cm), vitamin C (236.3 ~ 315.9 μ G/g) and soluble sugar (18.28 ~ 18.72mg/g) content is higher (table 1), can be used as novel vegetable application.
Compared with 1 wild cabbage of table and Chinese cabbage filial generation and parent tongue fur stem thickness be thin and vitamin C soluble sugar
Chinese cabbage Wild cabbage Synthetic rapeseed
Tongue fur stem thickness is thin (cm) 18.83±1.63b 17.62±1.81b 30.23±3.14a
Soluble sugar content (mg/g) in bud 16.03±2.38 b 21.81±3.64 a 18.72±2.40 ab
Soluble sugar content (mg/g) in stalk 10.95±2.25c 30.42±10.17 a 18.28±1.63b
Vitamin C content (μ g/g) in bud 167.4±41.05 b 390.3±59.57 a 315.9±51.76 a
Vitamin C content (μ g/g) in stalk 224.0±33.43 a 153.7±27.35 b 236.3±15.58 a
Note: abc letter is 0.05 level of signifiance
Embodiment 2
It (1) is female parent with Chinese cabbage, cabbage mustard is male parent, hybridization；
(2) Synthetic rapeseed (detailed in Example 1) is obtained after chromosome doubling；
(3) using Synthetic rapeseed and Natural Cabbage type napus hybrid, F is obtained1.Its growth potential is vigorous, tongue fur stem vitamin C (154.1 ~ 162.2 μ g/g) and soluble sugar (15.99 ~ 24.07mg/g) content are also higher (table 2), have and answer as novel vegetable Potentiality.
The synthesis of table 2 and Natural Cabbage type napus hybrid F1Tongue fur stem vitamin C soluble sugar is compared with Chinese cabbage wild cabbage
Chinese cabbage Wild cabbage Hybridize F1
Soluble sugar content (mg/g) in bud 16.03±2.38 b 21.81±3.64 a 15.99±1.80 b
Soluble sugar content (mg/g) in stalk 10.95±2.25b 30.42±10.17 a 24.07±1.81ab
Vitamin C content (μ g/g) in bud 167.4±41.05 b 390.3±59.57 a 154.1±18.98 b
Vitamin C content (μ g/g) in stalk 224.0±33.43 a 153.7±27.35 b 162.2±12.96 b
Note: abc letter is 0.05 level of signifiance.

Claims (4)

1. a kind of method using Chinese cabbage and wild cabbage distant hybridization creation novel vegetable, characterized in that the following steps are included:
(1) choosing vegetable with Chinese cabbage and wild cabbage is parent, and Post flowering selects fine day to carry out hybridization pollination；
(2) in pollination 15 days or so, after silique is expanded, silique is taken to carry out disinfection, strips rataria and be put into MS culture medium and cultivates To seedling；
(3) when the seedling 3-4 leaf phase, move into the MS of colchicine containing 50mg/L culture medium in cultivate one week or so, to hybrid seedling into Row chromosome doubling；
(4) it doubles rear seedling to then move to without in colchicine MS culture medium, when the leaf phase to be grown to 4-5, transplanting is planted in field Training.
2. the method according to claim 1 using Chinese cabbage and wild cabbage distant hybridization creation novel vegetable, characterized in that institute State in step (1) parent be vegetable with Chinese cabbage (B. rapa) or wild cabbage (B. oleracea) kind one or more of subspecies.
3. utilizing the method for Chinese cabbage and wild cabbage distant hybridization creation novel vegetable according to claim 1, characterized in that the step (3) culture medium can be added in colchicine by chromosome doubling in, directly processing test tube seedling.
4. utilizing the method for Chinese cabbage and wild cabbage distant hybridization creation novel vegetable according to claim 1, characterized in that the step (3) chromosome doubling can dig out plant in step (4) from soil in, be soaked root 6-8 hours with 1g/L colchicine solution, punching It is planted again after washing in field.
CN201810849131.5A 2018-07-28 2018-07-28 A method of novel vegetable is created using Chinese cabbage and wild cabbage distant hybridization Pending CN109042291A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112189563A (en) * 2020-10-19 2021-01-08 西南大学 Method for accelerating transformation of cytoplasmic male sterile line of brassica napus

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US20100222605A1 (en) * 2007-06-13 2010-09-02 Syngenta Participations Ag New hybrid system for brassica napus
CN102301946A (en) * 2011-07-26 2012-01-04 江苏省农业科学院 Method for creating yellow-seeded brassica napus germplasm
CN103190335A (en) * 2013-04-07 2013-07-10 湖南农业大学 Simple method for artificially synthesizing cabbage type rape
KR20140018028A (en) * 2012-08-03 2014-02-12 권오하 Brassica rapa. l plant with red color and method for breeding the same
CN106035059A (en) * 2016-05-26 2016-10-26 西北农林科技大学 Method for artificially synthesizing novel anti-clubroot brassica napus material
CN106134982A (en) * 2016-07-13 2016-11-23 河南省农业科学院经济作物研究所 The breeding method of cabbage type rape new lines
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Publication number Priority date Publication date Assignee Title
US20100222605A1 (en) * 2007-06-13 2010-09-02 Syngenta Participations Ag New hybrid system for brassica napus
CN102301946A (en) * 2011-07-26 2012-01-04 江苏省农业科学院 Method for creating yellow-seeded brassica napus germplasm
KR20140018028A (en) * 2012-08-03 2014-02-12 권오하 Brassica rapa. l plant with red color and method for breeding the same
CN103190335A (en) * 2013-04-07 2013-07-10 湖南农业大学 Simple method for artificially synthesizing cabbage type rape
CN106035059A (en) * 2016-05-26 2016-10-26 西北农林科技大学 Method for artificially synthesizing novel anti-clubroot brassica napus material
CN106134982A (en) * 2016-07-13 2016-11-23 河南省农业科学院经济作物研究所 The breeding method of cabbage type rape new lines
CN106613972A (en) * 2016-12-14 2017-05-10 江苏省农业科学院 Production method of club-root-resisting brassica oleracea-Chinese cabbage distant hybrid

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INOUE等: "Colchicine-induced tetraploid in Chinese cabbage（Brassica pekinensis RUPR.）", 《JAP.JOUR.GENET》 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112189563A (en) * 2020-10-19 2021-01-08 西南大学 Method for accelerating transformation of cytoplasmic male sterile line of brassica napus

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