CN109010662A - A kind of rush digestion growth promotion probiotics and preparation method thereof - Google Patents

A kind of rush digestion growth promotion probiotics and preparation method thereof Download PDF

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CN109010662A
CN109010662A CN201811276825.0A CN201811276825A CN109010662A CN 109010662 A CN109010662 A CN 109010662A CN 201811276825 A CN201811276825 A CN 201811276825A CN 109010662 A CN109010662 A CN 109010662A
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cfu
probiotics
bacillus
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张丽
刘应鹏
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Henan Hou Yi Industry Group Co Ltd
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    • A61K36/18—Magnoliophyta (angiosperms)
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Abstract

The present invention provides a kind of rush digestion growth promotion probiotics, the probiotics are by traditional Chinese medicinal components, prebiotics and fermenting microbe composition, the traditional Chinese medicinal components are composed of the following raw materials by weight: 50-60 parts of Divine Comedy, 40-55 parts of hawthorn, 40-50 parts of malt, 30-40 parts of radish seed, prebiotics are composed of the following raw materials by weight: 10-20 parts of galactooligosaccharide, 10-20 parts of xylo-oligosaccharide, 20-30 parts of oligoisomaltose, fermenting microbe includes 0.5-0.8 parts of S. cervisiae, 0.1-0.3 parts of bacillus subtilis, 0.05-0.2 parts of bacillus licheniformis, 0.05-0.2 parts of bacillus coagulans, 0.1-0.3 parts of clostridium butyricum, 0.01-0.02 parts of lactobacillus plantarum, lactobacillus acidophilus 0.02-0.0 4 parts, 0.02-0.04 parts of enterococcus faecium.The present invention is by eight kinds of probiotics, three kinds of prebiotics and four kinds of Chinese medicines through mixing, animal digestion can be helped to absorb, enhancing animal body resistance, prevention disease, save aquaculture cost, and it is easy to operate, utilization rate is high, feasibility is good, it is suitble to large-scale industrial production, has good promotion and application value.

Description

A kind of rush digestion growth promotion probiotics and preparation method thereof
Technical field
The present invention relates to a kind of poultry poultry micro-ecological preparations, and in particular to a kind of rush digestion growth promotion probiotics and its Preparation method.
Background technique
Promote Animal nutrition substance to digest and assimilate, improve food conversion ratio, be improved breeding performonce fo animals, saves and raise This is formed, has been more and more raisers problem of interest.Especially young animal digestive function and resistance development is endless Entirely, loss of appetite can be caused due to the sudden change of living condition, increase weight slow or even weight loss or illness, diarrhea of having loose bowels, The problems such as indigestion, and late growth is influenced, production cost is improved, causes serious economic loss to aquaculture.Due to Nonreactive green cultivation is advocated at present, and the safe natural additive such as probiotics is concerned.
Summary of the invention
To solve the above problems, the present invention provides a kind of rush digestion growth promotion probiotics and preparation method thereof.
To achieve the above object, described the present invention adopts the following technical scheme: a kind of rush digests growth promotion probiotics Probiotics are made of 160-205 parts of traditional Chinese medicinal components, 40-70 parts of prebiotics and 0.85-1.9 parts of fermenting microbes, the Chinese medicine Component is composed of the following raw materials by weight: 50-60 parts of Divine Comedy, 40-55 parts of hawthorn, 40-50 parts of malt, 30-40 parts of radish seed, benefit Raw member is composed of the following raw materials by weight: 10-20 parts of galactooligosaccharide, 10-20 parts of xylo-oligosaccharide, oligoisomaltose 20-30 Part, fermenting microbe includes 0.5-0.8 parts of S. cervisiae, 0.1-0.3 parts of bacillus subtilis, bacillus licheniformis 0.05-0.2 Part, 0.05-0.2 parts of bacillus coagulans, 0.1-0.3 parts of clostridium butyricum, 0.01-0.02 parts of lactobacillus plantarum, lactobacillus acidophilus 0.02-0.04 parts, 0.02-0.04 parts of enterococcus faecium.
Preferably, the probiotics are by 195 parts of traditional Chinese medicinal components, 50 parts of prebiotics and 1.275 parts of fermenting microbe groups At the traditional Chinese medicinal components are composed of the following raw materials by weight: 60 parts of Divine Comedy, 50 parts of hawthorn, 50 parts of malt, 35 parts of radish seed, benefit Raw member is composed of the following raw materials by weight: 10 parts of galactooligosaccharide, 10 parts of xylo-oligosaccharide, 30 parts of oligoisomaltose, zymophyte Kind include 0.6 part of S. cervisiae, 0.2 part of bacillus subtilis, 0.1 part of bacillus licheniformis, 0.1 part of bacillus coagulans, 0.2 part of clostridium butyricum, 0.015 part of lactobacillus plantarum, 0.03 part of lactobacillus acidophilus, 0.03 part of enterococcus faecium.
Fermenting microbe is made of the strain of following clump count: S. cervisiae 5.0 × 109-8.0×109CFU/g, withered grass bud Spore bacillus 2.0 × 109-5.0×109CFU/g, bacillus licheniformis 2.0 × 109-5.0×109CFU/g, bacillus coagulans 2.0×109-5.0×109CFU/g, clostridium butyricum 2.0 × 109-5.0×109CFU/g, lactobacillus plantarum 2.0 × 108-5.0× 108CFU/g, lactobacillus acidophilus 2.0 × 108-5.0×108CFU/g, enterococcus faecium 2.0 × 108-5.0×108CFU/g。
Preferably, fermenting microbe is made of the strain of following clump count: S. cervisiae 6.0 × 109CFU/g, withered grass Bacillus 2.0 × 109CFU/g, bacillus licheniformis 2.0 × 109CFU/g, bacillus coagulans 2.0 × 109CFU/g, butyric acid Clostridium 2.0 × 109CFU/g, lactobacillus plantarum 3.0 × 108CFU/g, lactobacillus acidophilus 3.0 × 108CFU/g, enterococcus faecium 3.0 ×108CFU/g。
Promote the preparation method of digestion growth promotion probiotics, comprising the following steps: (1) by S. cervisiae, withered grass bud Spore bacillus, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in the nutrient broth medium of 5L, shaking table culture It to 14-18h, is then inoculated in fermentor according to the additive amount of nutrient broth medium 3-5%, ferment 40-60h, obtains bacterium solution Ⅰ；(2) lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS culture medium, shaking table culture to 14-18h, then It is inoculated in fermentor according to the additive amount of MRS culture medium 3-5%, ferment 40-60h, obtains bacterium solution II；(3) by bacterium solution I and bacterium The addition protective agent of liquid II carries out spray drying and bacterium powder is made, spare；(4) weigh respectively the Divine Comedy of each parts by weight, hawthorn, malt, Radish seed, galactooligosaccharide, xylo-oligosaccharide and oligoisomaltose crushed 100-150 mesh；It (5) will be each in step (4) Raw material is added in the bacterium powder prepared, and mixing is sufficiently stirred, spare.
The protective agent is by 2-8 portions of skimmed milk powers, 10-20 portions of trehaloses, 2-8 parts of glycerol, 5-15 parts of shellfish spy's cyclodextrin groups At.
Preferably, the protective agent is by 5 portions of skimmed milk powers, 15 portions of trehaloses, 5 parts of glycerol, 10 parts of shellfish spy's cyclodextrin groups At.
Each effective component of the present invention has the advantage that be with beneficial effect:
Saccharomycete can secrete a variety of nutriments such as synthesis multivitamin, protein, amino acid, polypeptide and somatomedin, Animal nutrition is supplied, body growth is promoted.
Bacillus can secrete the protease of high activity, maltose, lipase, amylase, B- dextranase, plant A variety of enzymes such as sour enzyme and cellulase, can certain more complex carbohydrate in degrading plant feed, supplement in animal The deficiency of source enzyme, bacillus can be synthesized in the metabolic process of gastrointestinal tract multivitamin, protein, amino acid, polypeptide and A variety of nutriments such as somatomedin improve the digestibility and utilization rate of protein, starch, cellulose, calcium, phosphorus in feed.
Lactic acid bacteria secretes various digestive ferments in vivo, macro-molecular protein in feed can be degraded to polypeptide and amino acid, It reduces lactose as small molecule compound, is conducive to gastro-intestinal digestion and absorbs, the organic acid generated makes the elements such as calcium, phosphorus, iron be in easy The ionic condition of absorption, increases operation rate.
The physiological function of the oligomeric oligosaccharides of prebiotics is mainly to pass through the breeding for promoting enteral human body beneficial bacterium, in paraphyte Beneficial bacterium reduces harmful bacteria, and optimization flora carrys out the health of balanced human, and oligosaccharide also has the function of dietary fiber, can increase The retentiveness and capacity of stool play the role of " enteral street cleaner " to be easy to be discharged, and play two-way tune to constipation, diarrhea Section.
Divine Comedy is most widely used one kind in traditional zymotic class Chinese medicine on Chinese history, while being also earliest in history A kind of fungus solids fermented product.By in flour or wheat bran, rde bean, semen armeniacae amarae, fresh sweet wormwood, fresh polygonum flaccidum, six kinds of fresh Siberian cocklebur seedling Spontaneous fermentation forms medicine by a certain percentage, sweet in flavor, pungent, warm-natured, returns spleen, stomach meridian, and character is square or rectangular bulk, table Face khaki, coarse, matter is hard and crisp, frangibility, section are uneven, and off-white color has the faint scent of sweet wormwood, the visible rde bean having Exosper.Saccharomycete, mould, enzyme, ergosterol, vitamin B complex, volatile oil and glycoside etc. are mainly contained, is had There is invigorating the spleen warm stomach, help digestion lower gas, in tune the effect of antidiarrheal.
Hawthorn, sour, sweet, slightly warm in nature.Hawthorn is containing multivitamin, hawthorn acid, tartaric acid, citric acid, malic acid etc., also Containing the minerals such as flavonoids, lactone, carbohydrate, protein, fat and calcium, phosphorus, iron, contained lipolytic enzyme can promote fats to eat The digestion of object.Promote gastric secretion and increases the functions such as enzyme element in stomach.Chinese medicine thinks, hawthorn have stagnant disperse accumulationization, convergence and dysentery, Activate blood circulation and disperse blood clots and other effects.Cure mainly the diseases such as eating accumulation, chest diaphragm feeling of fullness, hernia blood stasis amenorrhoea.Contain mountain terpene and brass in hawthorn The drug ingedients such as class have significant expansion blood vessel and antihypertensive effect, have enhancing cardiac muscle, arrhythmia, adjust blood lipid and gallbladder The function of sterol content.
Malt, sweet and neutral.Returns spleen, stomach meridian.Promoting the circulation of qi helps digestion, spleen benefiting and stimulating the appetite, moves back newborn dissipate-swelling.For dyspepsia, stomach duct and abdomen is swollen Bitterly, spleen eating less, sweating, swollen breasts, weaning women, Fructus Hordei Germinatus strengthening the spleen and stomach promote lactation.For spleen eating less, newborn sweat is strongly fragrant Product.Coloured malt promoting the circulation of qi helps digestion delaactation.For dyspepsia, weaning women.Melanoidin malt relieving dyspepsia.For dyspepsia, stomach duct and abdomen Distending pain.
Radish seed, it is mild-natured, it is acrid flavour, sweet.Major function: help digestion and eliminate flatulence, lowering the adverse-rising QI to resolve phlegm.It is swollen for retention of food and drink, stomach duct and abdomen Bitterly, constipation, stagnation and diarrhea, phlegm choking, dyspnea and cough.Enter spleen, stomach, lung channel, can help digestion and eliminate flatulence, obvious effect, have " rush wall fall wall " it Claim.Clinic is commonly used in real (eating, the wet, stagnant) card for the treatment of.However, this product not be only help digestion and eliminate flatulence, to asthenic symptoms with it, obtain effect Also good, because of its property peace, smell not high, the disadvantage of unbiased victory again can not be limited to saying for " rushing wall fall wall ", there is the suspicion (reality of relieving stagnant Qi It is then having a surplus for flat gas), and give it up.
Rush digestion growth promotion probiotics of the invention are by eight kinds of probiotics, three kinds of prebiotics and four kinds of Chinese medicines through mixed It closes, animal digestion can be helped to absorb, enhancing animal body resistance, prevention disease, save aquaculture cost, and operate letter Just, utilization rate is high, feasibility is good, is suitble to large-scale industrial production, has good promotion and application value.
Specific embodiment
The present invention is further explained in the light of specific embodiments, but is not limited to following embodiment.
Embodiment 1
A kind of rush digestion growth promotion probiotics, probiotics are by 195 parts of traditional Chinese medicinal components, 50 parts of prebiotics and 1.275 parts Fermenting microbe composition, traditional Chinese medicinal components are composed of the following raw materials by weight: 60 parts of Divine Comedy, 50 parts of hawthorn, 50 parts of malt, radish seed 35 Part, prebiotics are composed of the following raw materials by weight: 10 parts of galactooligosaccharide, 10 parts of xylo-oligosaccharide, 30 parts of oligoisomaltose, hair Yeast-like fungi kind includes 0.6 part of S. cervisiae, 0.2 part of bacillus subtilis, 0.1 part of bacillus licheniformis, bacillus coagulans 0.1 Part, 0.2 part of clostridium butyricum, 0.015 part of lactobacillus plantarum, 0.03 part of lactobacillus acidophilus, 0.03 part of enterococcus faecium.
Fermenting microbe is made of the strain of following clump count: S. cervisiae 6.0 × 109CFU/g, bacillus subtilis 2.0×109CFU/g, bacillus licheniformis 2.0 × 109CFU/g, bacillus coagulans 2.0 × 109CFU/g, clostridium butyricum 2.0 ×109CFU/g, lactobacillus plantarum 3.0 × 108CFU/g, lactobacillus acidophilus 3.0 × 108CFU/g, enterococcus faecium 3.0 × 108CFU/g。
Promote the preparation method of digestion growth promotion probiotics, comprising the following steps: (1) by S. cervisiae, withered grass bud Spore bacillus, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in the nutrient broth medium of 5L, shaking table culture It to 16h, is then inoculated in fermentor according to the additive amount of nutrient broth medium 3%, ferment 48h, obtains bacterium solution I；(2) will Lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS culture medium, then shaking table culture to 16h is cultivated according to MRS The additive amount of base 3% is inoculated in fermentor, and ferment 48h, obtains bacterium solution II；(3) by bacterium solution I and bacterium solution II be added protective agent into Bacterium powder is made in row spray drying, and spare, protective agent is by 5 portions of skimmed milk powers, 15 portions of trehaloses, 5 parts of glycerol, 10 parts of shellfish spy's cyclodextrin Composition；(4) Divine Comedy, hawthorn, malt, radish seed, galactooligosaccharide, xylo-oligosaccharide and the oligomeric different wheat of each parts by weight are weighed respectively Bud sugar, crushed 120 meshes；(5) each raw material in step (4) is added in the bacterium powder prepared, mixing is sufficiently stirred, it is standby With.
Embodiment 2
A kind of rush digestion growth promotion probiotics, probiotics are by 160 parts of traditional Chinese medicinal components, 40 parts of prebiotics and 1.775 parts Fermenting microbe composition, traditional Chinese medicinal components are composed of the following raw materials by weight: 50 parts of Divine Comedy, 40 parts of hawthorn, 40 parts of malt, radish seed 30 Part, prebiotics are composed of the following raw materials by weight: 10 parts of galactooligosaccharide, 10 parts of xylo-oligosaccharide, 20 parts of oligoisomaltose, hair Yeast-like fungi kind includes 0.8 part of S. cervisiae, 0.3 part of bacillus subtilis, 0.15 part of bacillus licheniformis, bacillus coagulans 0.15 part, 0.3 part of clostridium butyricum, 0.015 part of lactobacillus plantarum, 0.03 part of lactobacillus acidophilus, 0.03 part of enterococcus faecium.
Fermenting microbe is made of the strain of following clump count: S. cervisiae 5.0 × 109CFU/g, bacillus subtilis 3.0×109CFU/g, bacillus licheniformis 3.0 × 109CFU/g, bacillus coagulans 3.0 × 109CFU/g, clostridium butyricum 4.0 ×109CFU/g, lactobacillus plantarum 4.0 × 108CFU/g, lactobacillus acidophilus 4.0 × 108CFU/g, enterococcus faecium 4.0 × 108CFU/g。
Promote the preparation method of digestion growth promotion probiotics, comprising the following steps: (1) by S. cervisiae, withered grass bud Spore bacillus, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in the nutrient broth medium of 5L, shaking table culture It to 15h, is then inoculated in fermentor according to the additive amount of nutrient broth medium 3%, ferment 45h, obtains bacterium solution I；(2) will Lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS culture medium, then shaking table culture to 15h is cultivated according to MRS The additive amount of base 3% is inoculated in fermentor, and ferment 45h, obtains bacterium solution II；(3) by bacterium solution I and bacterium solution II be added protective agent into Bacterium powder is made in row spray drying, and spare, protective agent is by 3 portions of skimmed milk powers, 10 portions of trehaloses, 3 parts of glycerol, 7 parts of shellfish spy's cyclodextrin Composition；(4) Divine Comedy, hawthorn, malt, radish seed, galactooligosaccharide, xylo-oligosaccharide and the oligomeric different wheat of each parts by weight are weighed respectively Bud sugar, crushed 120 meshes；(5) each raw material in step (4) is added in the bacterium powder prepared, mixing is sufficiently stirred, it is standby With.
Embodiment 3
A kind of rush digestion growth promotion probiotics, probiotics are by 180 parts of traditional Chinese medicinal components, 55 parts of prebiotics and 0.93 part of hair Yeast-like fungi kind composition, traditional Chinese medicinal components are composed of the following raw materials by weight: 55 parts of Divine Comedy, 45 parts of hawthorn, 45 parts of malt, radish seed 35 Part, prebiotics are composed of the following raw materials by weight: 15 parts of galactooligosaccharide, 15 parts of xylo-oligosaccharide, 25 parts of oligoisomaltose, hair Yeast-like fungi kind includes 0.5 part of S. cervisiae, 0.1 part of bacillus subtilis, 0.08 part of bacillus licheniformis, bacillus coagulans 0.1 part, 0.1 part of clostridium butyricum, 0.01 part of lactobacillus plantarum, 0.02 part of lactobacillus acidophilus, 0.02 part of enterococcus faecium.
Fermenting microbe is made of the strain of following clump count: S. cervisiae 7.0 × 109CFU/g, bacillus subtilis 4.0×109CFU/g, bacillus licheniformis 3.0 × 109CFU/g, bacillus coagulans 5.0 × 109CFU/g, clostridium butyricum 3.0 ×109CFU/g, lactobacillus plantarum 5.0 × 108CFU/g, lactobacillus acidophilus 2.0 × 108CFU/g, enterococcus faecium 5.0 × 108CFU/g。
Promote the preparation method of digestion growth promotion probiotics, comprising the following steps: (1) by S. cervisiae, withered grass bud Spore bacillus, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in the nutrient broth medium of 5L, shaking table culture It to 18h, is then inoculated in fermentor according to the additive amount of nutrient broth medium 5%, ferment 60h, obtains bacterium solution I；(2) will Lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS culture medium, then shaking table culture to 18h is cultivated according to MRS The additive amount of base 5% is inoculated in fermentor, and ferment 60h, obtains bacterium solution II；(3) by bacterium solution I and bacterium solution II be added protective agent into Bacterium powder is made in row spray drying, and spare, protective agent is by 8 portions of skimmed milk powers, 20 portions of trehaloses, 8 parts of glycerol, 15 parts of shellfish spy's cyclodextrin Composition；(4) Divine Comedy, hawthorn, malt, radish seed, galactooligosaccharide, xylo-oligosaccharide and the oligomeric different wheat of each parts by weight are weighed respectively Bud sugar, crushed 150 meshes；(5) each raw material in step (4) is added in the bacterium powder prepared, mixing is sufficiently stirred, it is standby With.
Embodiment 4
A kind of rush digestion growth promotion probiotics, probiotics are by 205 traditional Chinese medicinal components, 65 parts of prebiotics and 1.9 parts of fermentations Strain composition, traditional Chinese medicinal components are composed of the following raw materials by weight: 60 parts of Divine Comedy, 55 parts of hawthorn, 40 parts of malt, 40 parts of radish seed, Prebiotics are composed of the following raw materials by weight: 15 parts of galactooligosaccharide, 20 parts of xylo-oligosaccharide, 30 parts of oligoisomaltose, fermentation Strain includes 0.8 part of S. cervisiae, 0.3 part of bacillus subtilis, 0.2 part of bacillus licheniformis, bacillus coagulans 0.2 Part, 0.3 part of clostridium butyricum, 0.02 part of lactobacillus plantarum, 0.04 part of lactobacillus acidophilus, 0.04 part of enterococcus faecium.
Fermenting microbe is made of the strain of following clump count: S. cervisiae 8.0 × 109CFU/g, bacillus subtilis 5.0×109CFU/g, bacillus licheniformis 5.0 × 109CFU/g, bacillus coagulans 2.0 × 109CFU/g, clostridium butyricum 5.0 ×109CFU/g, lactobacillus plantarum 2.0 × 108CFU/g, lactobacillus acidophilus 5.0 × 108CFU/g, enterococcus faecium 2.0 × 108CFU/g。
Promote the preparation method of digestion growth promotion probiotics, comprising the following steps: (1) by S. cervisiae, withered grass bud Spore bacillus, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in the nutrient broth medium of 5L, shaking table culture It to 14h, is then inoculated in fermentor according to the additive amount of nutrient broth medium 4%, ferment 40h, obtains bacterium solution I；(2) will Lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS culture medium, then shaking table culture to 14h is cultivated according to MRS The additive amount of base 4% is inoculated in fermentor, and ferment 40h, obtains bacterium solution II；(3) by bacterium solution I and bacterium solution II be added protective agent into Bacterium powder is made in row spray drying, and spare, protective agent is by 2 portions of skimmed milk powers, 10 portions of trehaloses, 2 parts of glycerol, 5 parts of shellfish spy's cyclodextrin Composition；(4) Divine Comedy, hawthorn, malt, radish seed, galactooligosaccharide, xylo-oligosaccharide and the oligomeric different wheat of each parts by weight are weighed respectively Bud sugar, crushing sieve with 100 mesh sieve；(5) each raw material in step (4) is added in the bacterium powder prepared, mixing is sufficiently stirred, it is standby With.
Embodiment 5
Influence of the probiotics to Growth Performance of Weaning Piglets
It selects the ternary hybrid weanling pig that health similar in 100 35 age in days weight is grown up and is randomly divided into 5 groups, control group fed Basic drawing, 4 test groups add 2% embodiment 1,4 institute of embodiment 2, embodiment 3 and embodiment in basal diet respectively The product of preparation, using average daily gain, average daily gain, feed-weight ratio, diarrhea rate and apparent digestibility as index, research 4 Influence of the kind scheme to Growth Performance of Weaning Piglets, determines optimum combination.
One each group Growth Performance of Weaning Piglets of table compares
Two each group weanling pig apparent digestibility of table compares
The experimental results showed that 2 groups of embodiment and embodiment 4 are relative to control group and other two test groups, average daily gain is put down Equal daily ingestion amount significantly improves (P < 0.05), and feed-weight ratio and diarrhea rate significantly reduce (P < 0.05), example 1 group and embodiment 3 groups are not significantly different (P > 0.05) compared with control group.For feed apparent digestibility index, 2 groups of embodiment and 4 groups of embodiment Relative to control group and other two test groups, to dry matter, the digestibility of crude fibre and phosphorus is significantly improved (P < 0.05), real 4 groups of example are applied compared with other 4 groups, is significantly increased (P < 0.05) to nutritive digestibility, and example 1 group, 3 groups of embodiment and Without significant changes (P > 0.05) between control group.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art, the present invention It is not restricted to the described embodiments, under the premise of not departing from general idea of the present invention, several changes and improvements can also be made, These also should be regarded as protection scope of the present invention.

Claims (7)

1. a kind of rush digests growth promotion probiotics, it is characterised in that: the probiotics are by 160-205 parts of Chinese medicine groups Divide, 40-70 parts of prebiotics and 0.85-1.9 parts of fermenting microbes compositions, the traditional Chinese medicinal components are composed of the following raw materials by weight: mind 50-60 parts bent, 40-55 parts of hawthorn, 40-50 parts of malt, 30-40 parts of radish seed, prebiotics are composed of the following raw materials by weight: low Poly- galactolipin 10-20 parts, 10-20 parts of xylo-oligosaccharide, 20-30 parts of oligoisomaltose, fermenting microbe includes S. cervisiae 0.5-0.8 parts, 0.1-0.3 parts of bacillus subtilis, 0.05-0.2 parts of bacillus licheniformis, bacillus coagulans 0.05-0.2 Part, 0.1-0.3 parts of clostridium butyricum, 0.01-0.02 parts of lactobacillus plantarum, 0.02-0.04 parts of lactobacillus acidophilus, enterococcus faecium 0.02-0.04 parts.
2. as described in claim 1 promote digestion growth promotion probiotics, it is characterised in that: the probiotics are by 195 Part traditional Chinese medicinal components, 50 parts of prebiotics and 1.275 parts of fermenting microbes compositions, the traditional Chinese medicinal components are composed of the following raw materials by weight: 60 parts of Divine Comedy, 50 parts of hawthorn, 50 parts of malt, 35 parts of radish seed, prebiotics are composed of the following raw materials by weight: galactooligosaccharide 10 Part, 10 parts of xylo-oligosaccharide, 30 parts of oligoisomaltose, fermenting microbe include 0.6 part of S. cervisiae, bacillus subtilis 0.2 Part, 0.1 part of bacillus licheniformis, 0.1 part of bacillus coagulans, 0.2 part of clostridium butyricum, 0.015 part of lactobacillus plantarum, acidophilus cream 0.03 part of bacillus, 0.03 part of enterococcus faecium.
3. as claimed in claim 1 or 2 promote digestion growth promotion probiotics, it is characterised in that: fermenting microbe is by following bacterium Fall several strain composition: S. cervisiae 5.0 × 109-8.0×109CFU/g, bacillus subtilis 2.0 × 109-5.0× 109CFU/g, bacillus licheniformis 2.0 × 109-5.0×109CFU/g, bacillus coagulans 2.0 × 109-5.0×109CFU/g、 Clostridium butyricum 2.0 × 109-5.0×109CFU/g, lactobacillus plantarum 2.0 × 108-5.0×108CFU/g, lactobacillus acidophilus 2.0 ×108-5.0×108CFU/g, enterococcus faecium 2.0 × 108-5.0×108CFU/g。
4. as claimed in claim 3 promote digestion growth promotion probiotics, it is characterised in that: fermenting microbe is by following clump count Strain composition: S. cervisiae 6.0 × 109CFU/g, bacillus subtilis 2.0 × 109CFU/g, bacillus licheniformis 2.0 × 109CFU/g, bacillus coagulans 2.0 × 109CFU/g, clostridium butyricum 2.0 × 109CFU/g, lactobacillus plantarum 3.0 × 108CFU/g, lactobacillus acidophilus 3.0 × 108CFU/g, enterococcus faecium 3.0 × 108CFU/g。
5. a kind of prepare the as described in claim 1 method for promoting digestion growth promotion probiotics, it is characterised in that: including with Lower step: (1) S. cervisiae, bacillus subtilis, bacillus licheniformis, bacillus coagulans, clostridium butyricum are inoculated in In the nutrient broth medium of 5L, then shaking table culture to 14-18h is inoculated with according to the additive amount of nutrient broth medium 3-5% In fermentor, ferment 40-60h, obtains bacterium solution I；(2) lactobacillus plantarum, lactobacillus acidophilus, enterococcus faecium are inoculated in MRS In culture medium, then shaking table culture to 14-18h is inoculated in fermentor according to the additive amount of MRS culture medium 3-5%, and ferment 40- 60h obtains bacterium solution II；(3) bacterium solution I and the addition protective agent of bacterium solution II are carried out spray drying and bacterium powder is made, it is spare；(4) respectively Divine Comedy, hawthorn, malt, radish seed, galactooligosaccharide, xylo-oligosaccharide and the oligoisomaltose for weighing each parts by weight, crushed 100-150 mesh；(5) each raw material in step (4) is added in the bacterium powder prepared, mixing is sufficiently stirred, it is spare.
6. the preparation method as claimed in claim 5 for promoting digestion growth promotion probiotics, it is characterised in that: the protective agent It is made of 2-8 portions of skimmed milk powers, 10-20 portions of trehaloses, 2-8 parts of glycerol, 5-15 parts of shellfish spy's cyclodextrin.
7. the preparation method as claimed in claim 6 for promoting digestion growth promotion probiotics, it is characterised in that: the protective agent It is made of 5 portions of skimmed milk powers, 15 portions of trehaloses, 5 parts of glycerol, 10 parts of shellfish spy's cyclodextrin.
CN201811276825.0A 2018-10-30 2018-10-30 A kind of rush digestion growth promotion probiotics and preparation method thereof Pending CN109010662A (en)

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CN113061558A (en) * 2021-05-10 2021-07-02 长沙和光生物科技有限公司 Composite probiotics and feed additive containing bacillus coagulans HALO178
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Application publication date: 20181218