CN109006273A - It is a kind of improve corn seed salt tolerance culture solution and its application - Google Patents

It is a kind of improve corn seed salt tolerance culture solution and its application Download PDF

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Publication number
CN109006273A
CN109006273A CN201810937383.3A CN201810937383A CN109006273A CN 109006273 A CN109006273 A CN 109006273A CN 201810937383 A CN201810937383 A CN 201810937383A CN 109006273 A CN109006273 A CN 109006273A
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China
Prior art keywords
corn seed
salt tolerance
culture solution
seed salt
improving corn
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CN201810937383.3A
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Chinese (zh)
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CN109006273B (en
Inventor
李宗新
刘开昌
张慧
高英波
薛艳芳
钱欣
代红翠
张华斌
温立玉
韩小伟
于正贵
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Maize Research Institute of Shandong Academy of Agricultural Sciences
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Maize Research Institute of Shandong Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention belongs to crop planting technical fields, and in particular to a kind of culture solution for improving corn seed salt tolerance, and a kind of method for effectively improving corn seed salt tolerance is further disclosed.Culture solution of the present invention is with HgCl2It is active constituent with choline chloride, impregnating active is carried out to the corn seed by culture solution, the salt resistant character of corn seed can be effectively improved, the plantation yield under saline-alkali environment greatly improves.Culture solution of the present invention further adds cholinesterase, further improves the salt tolerance that the culture solution improves corn seed, and salt affected soil plantation yield further increases.

Description

It is a kind of improve corn seed salt tolerance culture solution and its application
Technical field
The invention belongs to crop planting technical fields, and in particular to a kind of culture solution for improving corn seed salt tolerance, And a kind of method for effectively improving corn seed salt tolerance is further disclosed.
Background technique
Corn also known as maize, ear of maize, maize etc. belong to grass family Zea.Corn rich in carbohydrate, protein, The human bodies required nutriment such as vitamin, dietary fiber, minerals, especially its luteole rich in, linoleic acid and Glutathione.Have shown that luteole be the constituent of human retina, linoleic acid is the required rouge of needed by human body in relation to research Fat acid, glutathione have with raising immunity of organisms and anticancer function, dietary fiber delays blood glucose rise and removing enteron aisle The functions such as rubbish.It may be said that corn whole body is treasured, play a significant role to human body abnormal metabolism is adjusted.
It is important grain required for world population that corn, which is important cereal crops, forage crop, light industry crop, One of one of food and the raw material of industry, and be distributed widest cereal crops in the world, cultivated area is only second to wheat and water Rice two occupies third position.China is located in corn gold growth band, is the second-biggest-in-the-world maize production and consumption for being only second to the U.S. Big country, in China, corn is even more to have evolved into the first big industrial crops.
The salinization of soil is a global resource and ecological problem, is the main abiotic stress factor of agricultural production One of.It is 3.6 × 10 that, which there is varieties in saline-alkali areas area in China,7hm2, wherein the salinized soil area about 1.3 with agricultural use potentiality × 107hm2, account for about the 10% of total area under cultivation, be mainly distributed on arid, semiarid zone and coastal region.Excavate crop itself Salt resistance ability, screening salt-enduring cultivars are considered as accelerating to utilize salt affected soil approach the most cost-effective.With Soil Secondary Salinization of soil phenomenon is on the rise, and the salinization of soil, which has become, restricts one of main factor of salt-soda soil maize production.Therefore, carry out The salt tolerance overall merit and salt-enduring cultivars of corn germ plasm resource are screened, and improve the salt tolerance of corn seed, for effective Using salt-soda soil, salt-soda soil maize culture is promoted to have important practical significance.
Summary of the invention
For this purpose, technical problem to be solved by the present invention lies in provide a kind of culture solution for improving corn seed salt tolerance;
Second technical problem to be solved by this invention is to provide a kind of side for effectively improving corn seed salt tolerance Method.
In order to solve the above technical problems, a kind of culture solution for improving corn seed salt tolerance of the present invention, including such as Lower component: HgCl21-2g/L, choline chloride 0.5-1.2g/L.
The culture solution of the raising corn seed salt tolerance, the component including following mass concentration: HgCl21.5g/L、 Choline chloride 0.8g/L.
The culture solution of the raising corn seed salt tolerance, further includes the cholinesterase of 0.1-0.3g/L.
The culture solution of the raising corn seed salt tolerance, the concentration of the cholinesterase are 0.2g/L.
The invention also discloses the culture solutions of the raising corn seed salt tolerance to improve corn seed salt tolerance neck Purposes in domain.
It is described including corn seed to be impregnated in the invention also discloses a kind of method for improving corn seed salt tolerance Improve the step in the culture solution of corn seed salt tolerance.
The solid-liquid ratio of the corn seed and the culture solution is 200-500g/L.
The dip time of the impregnation steps is 2-5h.
The impregnation steps are to carry out under room temperature.
The frequency of the ultrasonic step is 20-25KHZ.
Culture solution of the present invention is with HgCl2It is active constituent with choline chloride, by culture solution to the corn seed Impregnating active is carried out, the salt resistant character of corn seed can be effectively improved, the plantation yield under saline-alkali environment greatly improves.This It invents the culture solution and further adds cholinesterase, further improve the salt tolerant that the culture solution improves corn seed Property, salt affected soil plantation yield further increases.
Culture solution of the present invention, which only passes through dipping, can be realized the activation raising of corn seed salt tolerance, and further lead to The mode impregnated under ultrasound condition is crossed, its salt resistant character is further increased.
Specific embodiment
Embodiment 1
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21g/L, chlorination gallbladder Alkali 1.2g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 200g/L Enter into the culture solution, 5h is impregnated under room temperature, and clean with clear water.
Embodiment 2
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment22g/L, chlorination gallbladder Alkali 0.5g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 500g/L Enter into the culture solution, 2h is impregnated under room temperature, and clean with clear water.
Embodiment 3
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, 3h is impregnated under room temperature, and clean with clear water.
Embodiment 4
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L, cholinesterase 0.1g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, 3h is impregnated under room temperature, and clean with clear water.
Embodiment 5
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L, cholinesterase 0.3g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, 3h is impregnated under room temperature, and clean with clear water.
Embodiment 6
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L, cholinesterase 0.2g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, 3h is impregnated under room temperature, and clean with clear water.
Embodiment 7
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L, cholinesterase 0.2g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, in room temperature, (frequency 20KHZ) impregnates 3h under ultrasound condition, and is cleaned with clear water.
Embodiment 8
The culture solution of corn seed salt tolerance, including following component: HgCl are improved described in the present embodiment21.5g/L, chlorination Choline 0.8g/L, cholinesterase 0.2g/L.
The present embodiment selects the beautiful 339 kind corn seeds of nation to be handled, and adds corn seed according to solid-liquid ratio 350g/L Enter into the culture solution, in room temperature, (frequency 25KHZ) impregnates 3h under ultrasound condition, and is cleaned with clear water.
Experimental example
Indoor water culture experiment is in May, 2015 to June in wheat and corn national engineering laboratory of Shandong Academy of Agricultural Sciences It carries out.Crop field qualification test in May, 2015 in October, 2016 is in Huanghe delta doctor Hui Bang section of Shandong Academy of Agricultural Sciences Work station (118 ° of 37'E, 37 ° of 47'N) progress is ground, experimental field soil is saline-alkali soil, salt content 2.49 ‰, 0-40cm soil foundation Nutrient situation is pH value 8.0, the content of organic matter 18.4g/kg, full nitrogen 1.1g/kg, available nitrogen 24.8mg/kg, rapid available phosphorus 10.1mg/kg, available potassium 168.5mg/kg.
The test of field situ identification, is all made of completely randomized design, zone leader 15m, line-spacing 65cm, each kind kind two seasons 12 rows are planted, planting density is 60000 plants/hm2, it is repeated 3 times.Preceding stubble Wheat stalk-covering returning to the field, no-tillage mechanical planting apply nitrogen 240kg/hm2, P2O560kg/hm2, K2O 180kg/hm2, other cultivation managements carry out by common crop field high yield requirement.
Field yield measurement: removing flash trimming row, and every 4 row of cell harvesting corn (every row 5m) claims all fruit ears total fresh weight, by flat Fresh fringe weight randomly selects 20 fringes from received fruit ear, and indoor species test, investigation spike length, fringe is thick, ear barren tip is long, tassel row number, row grain number And mass of 1000 kernel, while grain water content is measured, actual production (by 14% conversion moisture content) is calculated, and record.
1, identical corn variety treatment effect
With treated in above-described embodiment 1-8, beautiful 339 corn seeds of nation carry out salt-soda soil plantation respectively, and with untreated Beautiful 339 corn seeds of nation carry out plantation control, in the manner described above with its actual production of criterion calculation and be recorded in the following table 1.
The identical corn variety treatment effect of table 1
From 1 data of table it is found that culture solution of the present invention can effectively improve the salt resistant character of corn seed, it is more suitable for salt It plants to alkali and is used.
2, different corn variety treatment effects
Selecting the featured corn variety in 30, Huang-Huai-Hai summer corn area is material to be tested (see the table below 2).
Selected difference corn variety seeds 50, impregnate 2h with culture solution described in embodiment 6, then use distilled water flushing 3- It is 5 times, spare (experimental group);And it is tested as a control group with the corn seed being not handled by.In the manner described above and mark Standard calculates its actual production and is recorded in the following table 2.
The different corn variety treatment effects of table 2
From upper table data it is found that under field condition, with culture solution of the present invention to 30 corn varieties of existing routine Salt tolerance processing is carried out, the yield performance in salt-soda soil increases.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or It changes still within the protection scope of the invention.

Claims (10)

1. a kind of culture solution for improving corn seed salt tolerance, which is characterized in that including following component: HgCl21-2g/L, chlorination Choline 0.5-1.2g/L.
2. the culture solution according to claim 1 for improving corn seed salt tolerance, which is characterized in that dense including following quality The component of degree: HgCl21.5g/L, choline chloride 0.8g/L.
3. the culture solution according to claim 1 or 2 for improving corn seed salt tolerance, which is characterized in that further include 0.1- The cholinesterase of 0.3g/L.
4. the culture solution according to claim 3 for improving corn seed salt tolerance, which is characterized in that the cholinesterase Concentration is 0.2g/L.
5. the described in any item culture solutions for improving corn seed salt tolerance of claim 1-4 are improving corn seed salt tolerance neck Purposes in domain.
6. a kind of method for improving corn seed salt tolerance, which is characterized in that including corn seed is impregnated in claim 1-4 Step in described in any item culture solutions for improving corn seed salt tolerance.
7. the method according to claim 6 for improving corn seed salt tolerance, which is characterized in that the corn seed and institute The solid-liquid ratio for stating culture solution is 200-500g/L.
8. the method according to claim 6 or 7 for improving corn seed salt tolerance, which is characterized in that the impregnation steps Dip time be 2-5h.
9. according to the described in any item methods for improving corn seed salt tolerance of claim 6-8, which is characterized in that the dipping Step is to carry out under room temperature.
10. the method according to claim 9 for improving corn seed salt tolerance, which is characterized in that the ultrasonic step Frequency is 20-25KHZ.
CN201810937383.3A 2018-08-16 2018-08-16 Culture solution for improving salt tolerance of corn seeds and application thereof Active CN109006273B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111418612A (en) * 2020-05-29 2020-07-17 山东省农业科学院作物研究所 Regulating agent for improving salt tolerance of crops and preparation method thereof
CN113924840A (en) * 2021-10-11 2022-01-14 温州科技职业学院 Seed soaking and germination accelerating method capable of improving performance of rice seeds

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JPH0638744A (en) * 1992-07-21 1994-02-15 Nakano Vinegar Co Ltd New heat-resistant glutamic acid dehydrogenase and its production
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CN106717289A (en) * 2017-03-01 2017-05-31 民勤县全盛永泰农业有限公司 A kind of method for improving arid salt-soda soil edible sunflower percentage of seedgermination
US20170158598A1 (en) * 2012-12-19 2017-06-08 Dow Agrosciences Llc Pesticidal compositions and processes related thereto
CN106879471A (en) * 2017-04-10 2017-06-23 云南天泉生物科技股份有限公司 A kind of bletilla tissue-cultured seedling production method of improvement
CN107172919A (en) * 2017-06-06 2017-09-19 山东省农业科学院玉米研究所 A kind of method for improving corn germ plasm resource seed Saline alkali tolerance energy
CN107820779A (en) * 2017-11-20 2018-03-23 蚌埠市禹会区马城胡本号家庭农场 A kind of modification processing method of corn seed
CN107996574A (en) * 2018-01-18 2018-05-08 佛山市盈辉作物科学有限公司 A kind of plant growth regulator of choline chloride and indolebutyric acid
CN108271339A (en) * 2014-12-30 2018-07-10 靛蓝农业公司 Seed endophyte, compositions related and its application method between cultivar and species

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0638744A (en) * 1992-07-21 1994-02-15 Nakano Vinegar Co Ltd New heat-resistant glutamic acid dehydrogenase and its production
CN103589749A (en) * 2003-09-29 2014-02-19 孟山都技术有限公司 Methods for enhancing stress tolerance in plants
CN104010505A (en) * 2011-10-26 2014-08-27 陶氏益农公司 Pesticidal compositions and processes related thereto
US20170158598A1 (en) * 2012-12-19 2017-06-08 Dow Agrosciences Llc Pesticidal compositions and processes related thereto
CN103493802A (en) * 2013-10-10 2014-01-08 苏州雅玳生物科技有限公司 Agricultural biological additive and purpose thereof
CN108271339A (en) * 2014-12-30 2018-07-10 靛蓝农业公司 Seed endophyte, compositions related and its application method between cultivar and species
CN106717289A (en) * 2017-03-01 2017-05-31 民勤县全盛永泰农业有限公司 A kind of method for improving arid salt-soda soil edible sunflower percentage of seedgermination
CN106879471A (en) * 2017-04-10 2017-06-23 云南天泉生物科技股份有限公司 A kind of bletilla tissue-cultured seedling production method of improvement
CN107172919A (en) * 2017-06-06 2017-09-19 山东省农业科学院玉米研究所 A kind of method for improving corn germ plasm resource seed Saline alkali tolerance energy
CN107820779A (en) * 2017-11-20 2018-03-23 蚌埠市禹会区马城胡本号家庭农场 A kind of modification processing method of corn seed
CN107996574A (en) * 2018-01-18 2018-05-08 佛山市盈辉作物科学有限公司 A kind of plant growth regulator of choline chloride and indolebutyric acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111418612A (en) * 2020-05-29 2020-07-17 山东省农业科学院作物研究所 Regulating agent for improving salt tolerance of crops and preparation method thereof
CN113924840A (en) * 2021-10-11 2022-01-14 温州科技职业学院 Seed soaking and germination accelerating method capable of improving performance of rice seeds

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