CN109001016A - 一种用于体外诊断试剂的加样变色的样本稀释液 - Google Patents

一种用于体外诊断试剂的加样变色的样本稀释液 Download PDF

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CN109001016A
CN109001016A CN201810883355.8A CN201810883355A CN109001016A CN 109001016 A CN109001016 A CN 109001016A CN 201810883355 A CN201810883355 A CN 201810883355A CN 109001016 A CN109001016 A CN 109001016A
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宣生良
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One Drop Biotechnology (changzhou) Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/302—Stain compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G01N2001/386—Other diluting or mixing processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G01N2001/386—Other diluting or mixing processes
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Abstract

本发明公开了一种用于体外诊断试剂的加样变色的样本稀释液，属于医疗检测领域。所述稀释液中含有的成分以及浓度如下：氯化钠6.0‑10.0g/L，磷酸氢二钠1.9‑3.9g/L，氯化钾2.0‑3.0g/L，磷酸二氢钾1.6‑3.0g/L，Tris 0.1‑0.6g/L，溴百里酚蓝0.2‑0.7g/L，叠氮化钠0.4‑0.6g/L。本发明加样稀释后，稀释剂会发生明显的颜色变化，由黄绿色变成蓝色，应用于流式免疫荧光法检测，具有高通量，高灵敏度，并行检测等特点。

Description

一种用于体外诊断试剂的加样变色的样本稀释液
技术领域
本发明涉及一种用于体外诊断试剂的加样变色的样本稀释液，属于医疗检测领域。
背景技术
在样品检测领域，稀释剂作为一种缓冲溶剂广泛使用，其将样品稀释至指定浓度然后进行检测，由于检测过程中仅需要微量的样本，每次测试会进行多个样本测试，测试过程可能会存在漏稀释一个或多个样本，使用常规稀释剂在检测过程中难于发现，因为微量的样本对于总体积而言基本不存在差异，因此存在漏加以及重复添加的风险。
发明内容
本发明所要解决的技术问题在于：提供一种用于体外诊断试剂的加样变色的样本稀释液，它解决了目前的稀释液添加后不易识别、存在漏加以及重复添加的问题。
本发明所要解决的技术问题采取以下技术方案来实现：
一种用于体外诊断试剂的加样变色的样本稀释液，所述样本稀释液中含有的成分以及浓度如下：
氯化钠 6.0-10.0g/L，
磷酸氢二钠 1.9-3.9g/L，
氯化钾 2.0-3.0g/L，
磷酸二氢钾 1.6-3.0g/L，
Tris 0.1-0.6g/L，
溴百里酚蓝 0.2-0.7g/L，
叠氮化钠 0.4-0.6g/L。
作为优选实例，所述溴百里酚蓝 浓度为0.3-0.6g/L。
作为优选实例，所述Tris的浓度为0.15-0.5g/L。
本发明的有益效果是：加样稀释后，稀释剂会发生明显的颜色变化，由黄绿色变成蓝色。
具体实施方式
为了对本发明的技术手段、创作特征、达成目的与功效易于明白了解，下面结合具体实施例，进一步阐述本发明。
一种用于体外诊断试剂的加样变色的样本稀释液，所述稀释液中含有的成分以及浓度如下：
氯化钠 6.0-10.0g/L，
磷酸氢二钠 1.9-3.9g/L，
氯化钾 2.0-3.0g/L，
磷酸二氢钾 1.6-3.0g/L，
Tris 0.1-0.6g/L，
溴百里酚蓝 0.2-0.7g/L，
叠氮化钠 0.4-0.6g/L。
所述溴百里酚蓝 浓度为0.3-0.6g/L。
所述Tris的浓度为0.15-0.5g/L。
实施例一
稀释液中含有的成分以及浓度如下：氯化钠6.0g/L，磷酸氢二钠 1.9 g/L，氯化钾 2.0g/L，磷酸二氢钾 1.6 g/L，Tris 0.1g/L，溴百里酚蓝 0.2g/L，叠氮化钠 0.4g/L。
实施例二
稀释液中含有的成分以及浓度如下：氯化钠8.0g/L，磷酸氢二钠 2.5g/L，氯化钾2.2g/L，磷酸二氢钾 1.8 g/L，Tris 0.2g/L，溴百里酚蓝 0.3g/L，叠氮化钠 0.45g/L。
实施例三
稀释液中含有的成分以及浓度如下：氯化钠7.0g/L，磷酸氢二钠 2.8g/L，氯化钾2.4g/L，磷酸二氢钾2.2 g/L，Tris 0.3g/L，溴百里酚蓝 0.4g/L，叠氮化钠 0.5g/L。
实施例四
稀释液中含有的成分以及浓度如下：氯化钠9.0g/L，磷酸氢二钠 3.2g/L，氯化钾2.6g/L，磷酸二氢钾2.6 g/L，Tris 0.4g/L，溴百里酚蓝 0.5g/L，叠氮化钠 0.55g/L。
实施例五
稀释液中含有的成分以及浓度如下：氯化钠10.0g/L，磷酸氢二钠 3.9g/L，氯化钾3.0g/L，磷酸二氢钾2.8 g/L，Tris 0.5g/L，溴百里酚蓝 0.7g/L，叠氮化钠 0.6g/L。
实施例六
稀释液中含有的成分以及浓度如下：氯化钠8.0g/L，磷酸氢二钠 3.0g/L，氯化钾2.3g/L，磷酸二氢钾2.3g/L，Tris 0.15g/L，溴百里酚蓝 0.3g/L，叠氮化钠 0.5g/L。
实施例七
稀释液中含有的成分以及浓度如下：氯化钠7.0g/L，磷酸氢二钠 2.5g/L，氯化钾2.2g/L，磷酸二氢钾2.0g/L，Tris 0.5g/L，溴百里酚蓝 0.6g/L，叠氮化钠 0.5g/L。
本发明中，稀释液母液即为基础缓冲液。
本发明加样稀释后，稀释剂会发生明显的颜色变化，由黄绿色变成蓝色。
以上显示和描述了本发明的基本原理和主要特征和本发明的优点。本行业的技术人员应该了解，本发明不受上述实施例的限制，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，这些变化和改进都落入本发明要求保护的范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。

Claims (3)

1.一种用于体外诊断试剂的加样变色的样本稀释液，其特征在于：所述稀释液中含有的成分以及浓度如下：
氯化钠 6.0-10.0g/L，
磷酸氢二钠 1.9-3.9g/L，
氯化钾 2.0-3.0g/L，
磷酸二氢钾 1.6-3.0g/L，
Tris 0.1-0.6g/L，
溴百里酚蓝 0.2-0.7g/L，
叠氮化钠 0.4-0.6g/L。
2.根据权利要求1所述的一种用于体外诊断试剂的加样变色的样本稀释液，其特征在于:所述溴百里酚蓝 浓度为0.3-0.6g/L。
3.根据权利要求1所述的一种用于体外诊断试剂的加样变色的样本稀释液，其特征在于:所述Tris的浓度为0.15-0.5g/L。
CN201810883355.8A 2018-08-06 2018-08-06 一种用于体外诊断试剂的加样变色的样本稀释液 Withdrawn CN109001016A (zh)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1222677A (zh) * 1997-10-31 1999-07-14 中国人民武装警察部队江苏省总队医院 一种血细胞计数仪的稀释液、溶血剂清洗液及其制备方法
CN101603091A (zh) * 2009-05-22 2009-12-16 兰州慧盟生物科技有限公司 西瓜细菌性果斑病菌免疫pcr的检测试剂盒和试剂盒使用方法
US20100304998A1 (en) * 2009-06-02 2010-12-02 Marquette University Chemical Proteomic Assay for Optimizing Drug Binding to Target Proteins
CN102928597A (zh) * 2012-10-30 2013-02-13 北京维德维康生物技术有限公司 同时检测磺胺类和喹诺酮类药物的elisa方法及其试剂盒
CN103278631A (zh) * 2013-04-03 2013-09-04 中国农业科学院油料作物研究所 黄曲霉毒素b1流动滞后免疫时间分辨荧光速测试剂盒及其应用
CN103323308A (zh) * 2013-05-23 2013-09-25 北京博晖创新光电技术股份有限公司 一种处理微量全血的试剂及其应用
CN107677664A (zh) * 2017-08-23 2018-02-09 中粮（成都）粮油工业有限公司 一种稻谷新鲜度快速测试方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1222677A (zh) * 1997-10-31 1999-07-14 中国人民武装警察部队江苏省总队医院 一种血细胞计数仪的稀释液、溶血剂清洗液及其制备方法
CN101603091A (zh) * 2009-05-22 2009-12-16 兰州慧盟生物科技有限公司 西瓜细菌性果斑病菌免疫pcr的检测试剂盒和试剂盒使用方法
US20100304998A1 (en) * 2009-06-02 2010-12-02 Marquette University Chemical Proteomic Assay for Optimizing Drug Binding to Target Proteins
CN102928597A (zh) * 2012-10-30 2013-02-13 北京维德维康生物技术有限公司 同时检测磺胺类和喹诺酮类药物的elisa方法及其试剂盒
CN103278631A (zh) * 2013-04-03 2013-09-04 中国农业科学院油料作物研究所 黄曲霉毒素b1流动滞后免疫时间分辨荧光速测试剂盒及其应用
CN103323308A (zh) * 2013-05-23 2013-09-25 北京博晖创新光电技术股份有限公司 一种处理微量全血的试剂及其应用
CN107677664A (zh) * 2017-08-23 2018-02-09 中粮（成都）粮油工业有限公司 一种稻谷新鲜度快速测试方法

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