CN108949877B - 一种从大蒜中提取分离大蒜低聚肽的方法 - Google Patents
一种从大蒜中提取分离大蒜低聚肽的方法 Download PDFInfo
- Publication number
- CN108949877B CN108949877B CN201810764196.XA CN201810764196A CN108949877B CN 108949877 B CN108949877 B CN 108949877B CN 201810764196 A CN201810764196 A CN 201810764196A CN 108949877 B CN108949877 B CN 108949877B
- Authority
- CN
- China
- Prior art keywords
- garlic
- filtrate
- oligopeptide
- solution
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 240000002234 Allium sativum Species 0.000 title claims abstract description 88
- 235000004611 garlic Nutrition 0.000 title claims abstract description 88
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 51
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000000706 filtrate Substances 0.000 claims abstract description 79
- 239000000047 product Substances 0.000 claims abstract description 48
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 36
- 239000000463 material Substances 0.000 claims abstract description 31
- 238000001914 filtration Methods 0.000 claims abstract description 28
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 28
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 108091005804 Peptidases Proteins 0.000 claims abstract description 25
- 239000004365 Protease Substances 0.000 claims abstract description 25
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000967 suction filtration Methods 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 16
- 238000002791 soaking Methods 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 239000012065 filter cake Substances 0.000 claims description 24
- 238000005374 membrane filtration Methods 0.000 claims description 12
- 238000001728 nano-filtration Methods 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 238000000108 ultra-filtration Methods 0.000 claims description 10
- 108090000145 Bacillolysin Proteins 0.000 claims description 9
- 108091005507 Neutral proteases Proteins 0.000 claims description 9
- 102000035092 Neutral proteases Human genes 0.000 claims description 9
- 239000000919 ceramic Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 5
- 101710189683 Alkaline protease 1 Proteins 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 19
- 238000010298 pulverizing process Methods 0.000 abstract description 7
- 239000000243 solution Substances 0.000 description 27
- 239000002994 raw material Substances 0.000 description 19
- XUHLIQGRKRUKPH-GCXOYZPQSA-N Alliin Natural products N[C@H](C[S@@](=O)CC=C)C(O)=O XUHLIQGRKRUKPH-GCXOYZPQSA-N 0.000 description 17
- XUHLIQGRKRUKPH-UHFFFAOYSA-N S-allyl-L-cysteine sulfoxide Natural products OC(=O)C(N)CS(=O)CC=C XUHLIQGRKRUKPH-UHFFFAOYSA-N 0.000 description 17
- XUHLIQGRKRUKPH-DYEAUMGKSA-N alliin Chemical compound OC(=O)[C@@H](N)C[S@@](=O)CC=C XUHLIQGRKRUKPH-DYEAUMGKSA-N 0.000 description 17
- 235000015295 alliin Nutrition 0.000 description 17
- 230000000052 comparative effect Effects 0.000 description 17
- 239000012528 membrane Substances 0.000 description 14
- 235000018102 proteins Nutrition 0.000 description 13
- 102000004169 proteins and genes Human genes 0.000 description 13
- 108090000623 proteins and genes Proteins 0.000 description 13
- ZFAHNWWNDFHPOH-YFKPBYRVSA-N S-allylcysteine Chemical compound OC(=O)[C@@H](N)CSCC=C ZFAHNWWNDFHPOH-YFKPBYRVSA-N 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 12
- 239000008399 tap water Substances 0.000 description 12
- 235000020679 tap water Nutrition 0.000 description 12
- 239000002131 composite material Substances 0.000 description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 238000007605 air drying Methods 0.000 description 8
- 229920001184 polypeptide Polymers 0.000 description 8
- 102000004196 processed proteins & peptides Human genes 0.000 description 8
- 238000010992 reflux Methods 0.000 description 8
- 238000005303 weighing Methods 0.000 description 8
- 238000000926 separation method Methods 0.000 description 7
- ZFAHNWWNDFHPOH-UHFFFAOYSA-N S-Allyl-L-cystein Natural products OC(=O)C(N)CSCC=C ZFAHNWWNDFHPOH-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000002390 rotary evaporation Methods 0.000 description 6
- 238000001291 vacuum drying Methods 0.000 description 6
- 108091005658 Basic proteases Proteins 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- 239000008107 starch Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 150000005837 radical ions Chemical class 0.000 description 4
- 238000010025 steaming Methods 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 229910017053 inorganic salt Inorganic materials 0.000 description 2
- 238000005360 mashing Methods 0.000 description 2
- 239000012982 microporous membrane Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 235000019633 pungent taste Nutrition 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019604 hot taste sensations Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001433 sodium tartrate Substances 0.000 description 1
- 229960002167 sodium tartrate Drugs 0.000 description 1
- 235000011004 sodium tartrates Nutrition 0.000 description 1
- 235000019614 sour taste Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810764196.XA CN108949877B (zh) | 2018-07-12 | 2018-07-12 | 一种从大蒜中提取分离大蒜低聚肽的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810764196.XA CN108949877B (zh) | 2018-07-12 | 2018-07-12 | 一种从大蒜中提取分离大蒜低聚肽的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108949877A CN108949877A (zh) | 2018-12-07 |
CN108949877B true CN108949877B (zh) | 2020-09-29 |
Family
ID=64482919
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810764196.XA Active CN108949877B (zh) | 2018-07-12 | 2018-07-12 | 一种从大蒜中提取分离大蒜低聚肽的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108949877B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112760350A (zh) * | 2021-02-06 | 2021-05-07 | 巨野恒丰果蔬有限公司 | 一种大蒜降压肽的制备方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09309838A (ja) * | 1996-05-20 | 1997-12-02 | Riken Health Kk | 生体防御機能増強剤 |
JPH10306032A (ja) * | 1997-05-08 | 1998-11-17 | Riken Health Kk | 精力増強剤 |
CN102443617A (zh) * | 2011-11-18 | 2012-05-09 | 江苏大学 | 一种大蒜降血压功能因子的超声辅助酶解制备方法 |
CN102911992A (zh) * | 2012-10-17 | 2013-02-06 | 常州康和生物技术有限公司 | 一种酶解玉米蛋白制备玉米低聚肽螯合物的方法 |
CN103598544A (zh) * | 2013-11-19 | 2014-02-26 | 徐州绿之野生物食品有限公司 | 以大蒜为原料进行综合利用的提取工艺 |
CN106579462A (zh) * | 2016-12-15 | 2017-04-26 | 得利斯集团有限公司 | 一种黑蒜低聚肽的提取方法 |
CN108164611A (zh) * | 2017-11-28 | 2018-06-15 | 中国农业科学院农产品加工研究所 | 一种大蒜综合提取利用的方法 |
-
2018
- 2018-07-12 CN CN201810764196.XA patent/CN108949877B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH09309838A (ja) * | 1996-05-20 | 1997-12-02 | Riken Health Kk | 生体防御機能増強剤 |
JPH10306032A (ja) * | 1997-05-08 | 1998-11-17 | Riken Health Kk | 精力増強剤 |
CN102443617A (zh) * | 2011-11-18 | 2012-05-09 | 江苏大学 | 一种大蒜降血压功能因子的超声辅助酶解制备方法 |
CN102911992A (zh) * | 2012-10-17 | 2013-02-06 | 常州康和生物技术有限公司 | 一种酶解玉米蛋白制备玉米低聚肽螯合物的方法 |
CN103598544A (zh) * | 2013-11-19 | 2014-02-26 | 徐州绿之野生物食品有限公司 | 以大蒜为原料进行综合利用的提取工艺 |
CN106579462A (zh) * | 2016-12-15 | 2017-04-26 | 得利斯集团有限公司 | 一种黑蒜低聚肽的提取方法 |
CN108164611A (zh) * | 2017-11-28 | 2018-06-15 | 中国农业科学院农产品加工研究所 | 一种大蒜综合提取利用的方法 |
Non-Patent Citations (3)
Title |
---|
Garlic Allicin as a Potential Agent for Controlling Oral Pathogens;Bachrach, Gilad等;《JOURNAL OF MEDICINAL FOOD》;20111130;第14卷(第11期);第1338-1343页 * |
大蒜渣ACEI活性肽的制备及其稳定性研究;丁青芝等;《时珍国医国药》;20120131;第23卷(第1期);第87-90页 * |
膜分离提取大蒜SOD及其水溶性多肽研究;谌威;《化学工程与装备》;20081030(第10期);第14-18页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108949877A (zh) | 2018-12-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108653417B (zh) | 一种黑果花楸的提取方法及其提取物 | |
CN101461514A (zh) | 苦瓜提取物及其制备方法 | |
CN101744326A (zh) | 枸杞清汁及其制备方法 | |
CN112812196B (zh) | 一种利用大蒜萃取后的萃取液或大蒜切片水生产大蒜多糖的工业化方法 | |
CN106317243A (zh) | 一种枸杞多糖的制备方法 | |
KR100797016B1 (ko) | 면역증강 활성을 나타내는 인삼다당체 고농축정제액과 그제조방법 | |
CN109180755A (zh) | 一种从甜茶叶中提取甜茶苷的方法 | |
CN105693680B (zh) | 一种蓝靛果花青素的提取方法 | |
CN110183541A (zh) | 红车轴草多糖及总异黄酮的制备方法 | |
CN108949877B (zh) | 一种从大蒜中提取分离大蒜低聚肽的方法 | |
CN109593110B (zh) | 一种利用化橘红制备柚皮苷的方法 | |
CN115197287A (zh) | 综合提取甜茶中的甜茶苷、槲皮素和鞣花酸的方法及其应用 | |
CN109369733B (zh) | 一种从苦荞叶中同时提取多种黄酮类化合物的方法 | |
AU2017416080A1 (en) | Method for preparing betanin | |
CN115057900B (zh) | 一种从黑果腺肋花楸中提取花色苷的方法 | |
CN114712416B (zh) | 一种水媒法高效同步提取荷叶中黄酮、生物碱和多酚的方法 | |
CN116694711A (zh) | 一种高纯度牡蛎肽的制备方法 | |
CN114904294A (zh) | 一种高得率茶黄酮的制备方法 | |
RU2613294C1 (ru) | Способ получения меланина из лузги подсолнечника | |
CN113845564A (zh) | 刺梨抗氧化寡肽及其制备方法、应用和抗氧化制品 | |
CN111171170A (zh) | 一种绿萝花多糖的提取方法 | |
CN110585339A (zh) | 一种白芨多个有效成分的分离制备方法 | |
CN103141829A (zh) | 一种浓缩蒲公英汁的制备方法 | |
TWI828340B (zh) | 蜂膠的處理方法 | |
CN112106989A (zh) | 一种甘薯茎叶多酚及其提取方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240510 Address after: 712000, Unit 1-029, Building 3, Xi'an Airport Industrial Port, Zhongnan High Tech, No. 8 Zhaorong South Street, Airport New City, Xixian New District, Xi'an City, Shaanxi Province Patentee after: Shaanxi Jinweifang Biopharmaceutical Technology Co.,Ltd. Country or region after: China Patentee after: Li Duowei Address before: 4-3-2, building 9, science and technology enterprise accelerator Park, west 2, Qinling Avenue, Caotang science and technology industrial base, high tech Zone, Xi'an City, Shaanxi Province, 710000 Patentee before: XI'AN YOUTH HEALTHY BIOTECHNOLOGY Co.,Ltd. Country or region before: China Patentee before: Li Duowei |