CN108949621B - Microbial agent for removing TVOC and formaldehyde and preparation method thereof - Google Patents
Microbial agent for removing TVOC and formaldehyde and preparation method thereof Download PDFInfo
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- CN108949621B CN108949621B CN201810740122.2A CN201810740122A CN108949621B CN 108949621 B CN108949621 B CN 108949621B CN 201810740122 A CN201810740122 A CN 201810740122A CN 108949621 B CN108949621 B CN 108949621B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/38—Removing components of undefined structure
- B01D53/44—Organic components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01D2251/95—Specific microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/708—Volatile organic compounds V.O.C.'s
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- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention relates to a microbial agent for removing TVOC and formaldehyde, which comprises the following raw materials in parts by weight: 3-8 parts of EM composite strain, 1-5 parts of lactic acid bacteria, 3-7 parts of composite bacterial liquid, 9-18 parts of plant extract, 18-28 parts of molasses, 3-8 parts of peptone, 3-8 parts of beef extract, 3-8 parts of yeast extract, 3-8 parts of agar and 803-8 parts of tween; during preparation: (1) taking EM composite strain, lactobacillus and composite bacteria liquid, sterilizing, adding purified water, molasses and performing anaerobic fermentation; (2) taking plant extract, adding molasses and EM composite strain, lactobacillus and composite bacterial liquid, and inoculating the plant extract to the mixed culture medium in the step (1) according to a certain volume percentage for fermentation; (3) and (3) filtering the substance in the step (2), centrifuging the filtered bacterial liquid, taking the supernatant, and concentrating the supernatant. Through the test of an authority organization, the removal rate of formaldehyde and TVOC reaches 95%, and the invention has low manufacturing cost and simple working procedure and can be widely applied to the technical field of indoor and outdoor harmful gas removal.
Description
Technical Field
The invention relates to the technical field of toxic and harmful gas treatment in indoor and outdoor air environments, in particular to a microbial agent for removing TVOC and formaldehyde and a preparation method thereof.
Background
TVOC is an organic matter with saturated vapor pressure exceeding 133.32pa at room temperature, has boiling point between 50 ℃ and 250 ℃, has certain toxicity, irritation, carcinogenicity and special odor, affects skin and mucous membrane, and can cause acute damage to human body. The world health organization, the national academy of sciences/national research council, and other agencies have been emphasizing that TVOCs are an important class of air pollutants. The outdoor environment mainly comes from fuel combustion and transportation, and the indoor environment mainly comes from combustion products such as coal and natural gas, smoke of smoking, heating and cooking, building and decorative materials, furniture, household appliances, detergents and the like. Experts and others have pointed out that, because of their strong volatility, TVOCs generally volatilize 90% within 10 hours after paint application, while TVOCs in solvents release only 25% of the total amount during paint drying. Formaldehyde is also a volatile organic compound, but formaldehyde is easily soluble in water, is different from other volatile organic compounds, is mainly from indoor sources, and causes great harm to human bodies after long-term formaldehyde inhalation.
The technology aiming at removing formaldehyde and TVOC at present is still the main object of our research, and the removing means is still a blank in the prior art.
Disclosure of Invention
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a microbial agent for removing TVOC and formaldehyde comprises the following raw materials in parts by weight: 3-8 parts of EM composite strain, 1-5 parts of lactic acid bacteria, 3-7 parts of composite bacterial liquid, 9-18 parts of plant extract, 18-28 parts of molasses, 3-8 parts of peptone, 3-8 parts of beef extract, 3-8 parts of yeast extract, 3-8 parts of agar and 803-8 parts of tween.
Preferably, the compound bacterial liquid comprises EM bacteria, bacillus subtilis, photosynthetic bacteria and cellulase, and the plant extract is prepared by adding water into wheat bran, bean sprouts, tomatoes and starch, decocting at high temperature to obtain juice and cooling.
The invention also provides a technical scheme that: a preparation method of a microbial agent for removing TVOC and formaldehyde comprises the following steps:
(1) respectively sterilizing 3-8 parts of EM composite strain, 1-5 parts of lactic acid bacteria and 3-7 parts of composite bacterial liquid, adding 40 parts of purified water and 20 parts of molasses, and performing anaerobic fermentation and activation for 7 days to form a mixed fermentation culture medium;
(2) taking 10 parts of plant extract, adding 20-27 parts of molasses and EM composite strain, 1-3 parts of lactic acid bacteria and 3-7 parts of composite bacteria liquid, inoculating the mixture into the mixed fermentation culture medium in the step (1) according to the inoculation amount of 30% by volume percentage, performing shake cultivation for 7 days at the temperature of 30 ℃ and at the speed of 180r/min, then loading the mixture into an anaerobic fermentation tank for secondary anaerobic fermentation for 30 days, controlling the temperature within the range of 25-30 ℃ in the fermentation process, and adjusting the pH value to be 4.5-6;
(3) and (3) after the fermentation is finished, filtering the substance obtained by the fermentation in the step (2), centrifuging the filtered bacterium liquid for 20min at the rotating speed of 10000r/min, taking the supernatant, and concentrating the supernatant by 3 times to obtain the microbial agent for removing TVOC and formaldehyde.
After the microbial agent for removing formaldehyde and TVOC is adopted, the invention has the following advantages: by using the method for removing TVOC and formaldehyde, the removal rate reaches more than 95 percent, the national air environment quality standard is reached, and the problem that the removal rate is not high or can not be removed in the existing market removal technology is effectively solved; the invention has low production cost, simple manufacturing process, simple and convenient implementation means, easy operation and great market prospect; the invention has the advantages of no hormone, no toxicity, no harm, no pollution, no deterioration and high safety performance.
Detailed Description
The present invention is described in further detail below.
The first embodiment is as follows: a microbial agent for removing TVOC and formaldehyde comprises the following raw materials in parts by weight: 3 parts of EM composite strain, 1 part of lactic acid bacteria, 3 parts of composite bacteria liquid with EM bacteria, bacillus subtilis, photosynthetic bacteria and cellulase, 10 parts of extracting solution containing wheat bran, bean sprouts, tomatoes and starch liquid, 20 parts of molasses, 4 parts of peptone, 4 parts of beef extract, 4 parts of yeast extract, 4 parts of agar and 804 parts of tween.
The raw materials are utilized to prepare the microbial solution for removing formaldehyde and TVOC, and the preparation steps are as follows:
(1) respectively sterilizing 3 parts of EM composite strain, 1 part of lactic acid bacteria and 3 parts of composite bacteria liquid, adding 40 parts of purified water and 20 parts of molasses, and performing anaerobic fermentation and activation for 7 days to form a mixed fermentation culture medium;
(2) taking 10 parts of plant extract, adding 20 parts of molasses and EM composite strain, 1 part of lactic acid bacteria and 3 parts of composite bacteria liquid, inoculating the mixture into the mixed fermentation culture medium in the step (1) according to the inoculation amount of 30% of volume percentage, performing shake cultivation for 7 days at the temperature of 30 ℃ and at the speed of 180r/min, then loading the mixture into an anaerobic fermentation tank for secondary anaerobic fermentation for 30 days, controlling the temperature in the fermentation process within the range of 25-30 ℃, and adjusting the pH value to be 4.5-6;
(3) and (3) after the fermentation is finished, filtering the substance obtained by the fermentation in the step (2), centrifuging the filtered bacterium liquid for 20min at the rotating speed of 10000r/min, taking the supernatant, and concentrating the supernatant by 3 times to obtain the microbial agent for removing TVOC and formaldehyde.
When the microbial agent is used in concrete implementation, the microbial agent for removing formaldehyde and TVOC provided by the invention is put into a spraying instrument, diluted by water according to the proportion of 30 times, and sprayed on a position to be treated, and the removal rate reaches 95%.
In the action principle, the microbial agent for removing formaldehyde and TVOC provided by the invention combines the biological absorption and the reaction action of biological enzyme, so that TVOC and formaldehyde are fundamentally removed. In the process of interaction, the compound microbial agent is utilized to promote the transformation and dissolution of toxic and harmful and volatile organic substances such as TVOC and formaldehyde in a mode of generating oxidation, reduction, adsorption or degradation, and by treating the TVOC and formaldehyde organic compounds, organic compounds which are difficult to dissolve in air are oxidized and reduced under the action of microbial solution and plant extract, so that the purpose of removing TVOC and formaldehyde is achieved.
The removal effect of the invention for removing ammonia from TVOC and air is shown in Table 1:
Claims (2)
1. the microbial agent for removing TVOC and formaldehyde is characterized by comprising the following raw materials in parts by weight: 3-8 parts of EM composite strain, 1-5 parts of lactic acid bacteria, 3-7 parts of composite bacterial liquid, 9-18 parts of plant extract, 18-28 parts of molasses, 3-8 parts of peptone, 3-8 parts of beef extract, 3-8 parts of yeast extract, 3-8 parts of agar and 803-8 parts of tween;
the preparation method of the microbial agent comprises the following steps:
(1) respectively sterilizing 3-8 parts of EM composite strain, 1-5 parts of lactic acid bacteria and 3-7 parts of composite bacterial liquid, adding 40 parts of purified water and 20 parts of molasses, and performing anaerobic fermentation and activation for 7 days to form a mixed fermentation culture medium;
(2) taking 10 parts of plant extract, adding 20-27 parts of molasses and EM composite strain, 1-3 parts of lactic acid bacteria and 3-7 parts of composite bacteria liquid, inoculating the mixture into the mixed fermentation culture medium in the step (1) according to the inoculation amount of 30% by volume percentage, performing shake cultivation for 7 days at the temperature of 30 ℃ and at the speed of 180r/min, then loading the mixture into an anaerobic fermentation tank for secondary anaerobic fermentation for 30 days, controlling the temperature within the range of 25-30 ℃ in the fermentation process, and adjusting the pH value to be 4.5-6;
(3) and (3) after the fermentation is finished, filtering the substance obtained by the fermentation in the step (2), centrifuging the filtered bacterium liquid for 20min at the rotating speed of 10000r/min, taking the supernatant, and concentrating the supernatant by 3 times to obtain the microbial agent for removing TVOC and formaldehyde.
2. The microbial inoculant for removing TVOC and formaldehyde as claimed in claim 1, wherein said compound bacteria liquid comprises EM bacteria, Bacillus subtilis, photosynthetic bacteria and cellulase, said plant extract is prepared by decocting wheat bran, bean sprouts, tomatoes and starch with water at high temperature to obtain juice and cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810740122.2A CN108949621B (en) | 2018-07-07 | 2018-07-07 | Microbial agent for removing TVOC and formaldehyde and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810740122.2A CN108949621B (en) | 2018-07-07 | 2018-07-07 | Microbial agent for removing TVOC and formaldehyde and preparation method thereof |
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| CN108949621A CN108949621A (en) | 2018-12-07 |
| CN108949621B true CN108949621B (en) | 2022-08-30 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105000675A (en) * | 2015-07-13 | 2015-10-28 | 上海新张卫生用品有限公司 | Method for controlling toilet odor with microbial agent |
| CN105107001A (en) * | 2015-08-29 | 2015-12-02 | 湖南普泰尔微科环保科技有限公司 | Preparation method of efficient microorganism deodorant |
| CN107142231A (en) * | 2017-05-31 | 2017-09-08 | 大工（青岛）新能源材料技术研究院有限公司 | A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100551977B1 (en) * | 2005-02-14 | 2006-02-20 | 담양군 | Silver-ionized tabasheer and use thereof |
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- 2018-07-07 CN CN201810740122.2A patent/CN108949621B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105000675A (en) * | 2015-07-13 | 2015-10-28 | 上海新张卫生用品有限公司 | Method for controlling toilet odor with microbial agent |
| CN105107001A (en) * | 2015-08-29 | 2015-12-02 | 湖南普泰尔微科环保科技有限公司 | Preparation method of efficient microorganism deodorant |
| CN107142231A (en) * | 2017-05-31 | 2017-09-08 | 大工（青岛）新能源材料技术研究院有限公司 | A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon and preparation method thereof |
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