CN107142231A - A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon and preparation method thereof - Google Patents
A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon and preparation method thereof Download PDFInfo
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- CN107142231A CN107142231A CN201710405387.2A CN201710405387A CN107142231A CN 107142231 A CN107142231 A CN 107142231A CN 201710405387 A CN201710405387 A CN 201710405387A CN 107142231 A CN107142231 A CN 107142231A
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Abstract
The invention belongs to Techniques for Indoor Air Purification field, and in particular to a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon and preparation method thereof.Preparing the raw material of the probiotics includes following material：35 parts of Sphingol single-cell, 13 parts of Lactobacillus casei, 13 parts of bifidobacterium breve, 13 parts of lactobacillus acidophilus, 40 80 parts of medium component, 30 40 parts of trehalose, 10 20 parts of xylose, 80 100 parts of plant extraction liquid, 2,500 3000 parts of distilled water.The beneficial microbes such as Sphingol single-cell, Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus in the probiotics, some active materials are produced by its body metabolism, the volatile aromatic hydrocarbon pollutants such as formaldehyde, benzene, dimethylbenzene, the phenol that can be degraded in air ambient, suppress the breeding of harmful bacteria in environment, so as to improve ambient air quality；Action effect persistently, can reach the purpose for removing volatile contaminant.
Description
Technical field
The invention belongs to Techniques for Indoor Air Purification field, and in particular to a kind of micro- life for dispelling in-car formaldehyde and aromatic hydrocarbon
State preparation and preparation method thereof.
Background technology
The lifting realized with the improvement of people ' s living standards and to landscaping, people increase the need to upholstery
Ask, but contain the arene compound such as substantial amounts of formaldehyde and volatile benzene, phenol in finishing material, they have strong cause
Cancer and tumor promotion, are also one of potential mutagen, have had a strong impact on daily life.So, people increase
To the improvement consciousness of the pollutant such as formaldehyde, benzene, toluene in automobile.
At present, it is to the major measure that in-car gas pollutant is administered both at home and abroad：Cover closing method, ventilation method, plant
Thing extraction method, photocatalyst technology, negative aeroion technology etc..But due to by in-vehicle exposure gas source extensively, purification product and
Limitation of administering method etc. influences, PARA FORMALDEHYDE PRILLS(91,95) to dispel efficiency different, there are some drawbacks, constrain people's PARA FORMALDEHYDE PRILLS(91,95)
The selection of administering method.
(1) closing method is covered
Go to cover another smell using a kind of smell, the poisonous and hazardous aromatic hydrocarbon such as benzene, phenol is covered up, such as delicate fragrance
Agent etc..
Shortcoming：The noxious materials such as in-car formaldehyde, benzene, dimethylbenzene are not degraded, and the concentration of pollutant is not reduced,
Still there is toxicity.
(2) photocatalyst catalysis method
Photocatalyst method refers to that light-catalyzed reaction occurs under conditions of illumination, produces with very strong photoredox energy
The free hydroxy and active oxygen of power, the oxidable various formaldehyde of decomposition, nitrobenzene, toluene, phenol, anthracene etc. are poisonous and hazardous organic
Thing, and these organic pollutions are resolved into free of contamination H2O and CO2。
Shortcoming：Cost is higher, valuable product.
(3) anion method
The anion that gas discharge is produced is made by high pressure, the pollutants such as in-car formaldehyde, benzene of degrading, so as to reduce pollution
The concentration of thing.
Shortcoming：Pollution degradation species and less efficient, and product price is higher, it is necessary to which larger equipment, influence is in-car
Overall appearance.
(4) absorption method
Aromatic hydrocarbon, alkynes class poisonous and harmful substance are adsorbed by sorbing materials such as activated carbon, silica gel, reached in a short time
To a kind of method of dispelling abnormal flavor.
Shortcoming：The easy saturation of adsorbent, easily loses suction-operated, need to often change adsorbent.
(5) plant extraction method
Containing some chemical combination analytes with functions such as degradation of formaldehyde, benzene, phenol in plant, by extraction, purifying etc.
Technique produces plant extract formula liquid, is then sprayed in space, poisonous and harmful substance is chemically reacted rapidly with it, will
Noxious material is degraded to nontoxic, tasteless H2O and CO2, so as to purify air.
Shortcoming：Extraction, purifying process are cumbersome, and price is of a relatively high.
(6) ventilation method
By ventilation, exchanged in the short time with ambient atmos rapidly, reach the purpose of reduction poisonous gas.
Shortcoming：Pollutant is long deenergized period, it is impossible to thoroughly eliminates organic pollution release, easily causes secondary environmental pollution.
The content of the invention
To solve the above-mentioned problems in the prior art, the present invention, which is provided, a kind of dispels the micro- of in-car formaldehyde and aromatic hydrocarbon
Ecological agent and preparation method thereof, this method is mainly primary raw material using Sphingol single-cell and acidophil, realize PARA FORMALDEHYDE PRILLS(91,95),
The degradation treatment of the pollutants such as benzene, dimethylbenzene, action effect is lasting, pollution-free, can reduce the peculiar smell in surrounding air, realizes
Air cleaning, is effectively improved air quality.
The technical solution adopted by the present invention is as follows：
A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, preparing the raw material of the probiotics includes following thing
Matter：Sphingomonas.3-5 parts of Sphingol single-cell, 1-3 parts of Lactobacillus casei Lactobacillus casei, short bifid
1-3 parts of bacillus Bifidobacterium breve, 1-3 parts of lactobacillus acidophilus Lactobacillus acidophilus, training
Support 40-80 parts of based component, 30-40 parts of trehalose, 10-20 parts of xylose, 80-100 parts of plant extraction liquid, distilled water 2500-3000
Part.
The medium component includes aerobic fluid nutrient medium, aerobic solid medium, anaerobic liquid culture medium and anaerobism
Solid medium.
The Sphingol single-cell be aerobic fermentation, aerobic Liquid Culture based component be peptone 3.0g, beef extract 1g,
NaCl 10g, distilled water 1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
The aerobic solid culture based component is agar 15g, peptone 3.0g, beef extract 1g, NaCl 10g, distilled water
1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
The Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus are anaerobic fermentation, and anaerobic liquid medium component is
Dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80 0.1mL, 5% bean cake powder leachate 80mL, distillation
Water 1000mL, 115 DEG C, sterilize 20min.
The anaerobic solids medium component is agar 15g, dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO4
0.2g, Tween80 0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
The plant extraction liquid is that the one or more in lemon, banana, apple, pickled cucumbers, tomato are smashed, and adds distillation
Water boils 0.5h, is cooled to suction filtration after room temperature, takes supernatant standby.
A kind of preparation method of the probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon as described above, including following step
Suddenly：
(1) prepare：It is as described above to prepare oxygen liquid culture medium, aerobic solid medium, anaerobic liquid culture medium, detest
Oxygen solid medium and plant extraction liquid；
(2) activate：By Sphingol single-cell strain water-bath activation, dilution spread is in aerobic solid medium culture；Will be dry
Lactobacillus paracasei, bifidobacterium breve and Lactobacillus acidophilus species' difference water-bath activation, dilution spread is in anaerobic solids culture medium respectively
Culture；
(3) it is inoculated with：Picking Sphingol single-cell strain single bacterium colony is seeded to the purebred culture of aerobic fluid nutrient medium, and culture is extremely
Logarithmic phase；Picking Lactobacillus casei, bifidobacterium breve and Lactobacillus acidophilus species' single bacterium colony are seeded to anaerobic liquid respectively respectively
The purebred culture of culture medium, culture to logarithmic phase；
(4) fermented and cultured：The Sphingol single-cell of logarithmic phase is linked into aerobic fluid nutrient medium in 1%-5% ratio
In, sealing is passed through filtrated air, fermented and cultured；By the Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus of logarithmic phase, press
1%-5% ratio is linked into anaerobic liquid culture medium, sealing, fermented and cultured；
(5) centrifuge：Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution after having fermented, centrifuge, obtain supernatant respectively, mixing；
(6) it is filling：Add trehalose, xylose, plant extraction liquid to be sufficiently mixed, working solution is made filling.
In the step (2), the water-bath activation condition of Sphingol single-cell is：37 DEG C, activate 1-2min, condition of culture
For：28-37 DEG C, cultivate 24h；Lactobacillus casei, bifidobacterium breve and the water-bath of lactobacillus acidophilus activation condition are：It is 37 DEG C, living
Change 1-2min, condition of culture is：28-37 DEG C, cultivate 24h.
In the step (3), the purebred condition of culture of Sphingol single-cell is：28-37 DEG C, 50-80r/min；Cheese breast
The purebred condition of culture of bacillus, bifidobacterium breve and lactobacillus acidophilus is：28-37 DEG C, 50-80r/min.
In the step (4), the fermentation culture conditions of Sphingol single-cell are：28-37 DEG C, 150-180r/min fermentations,
4-7d is cultivated, to pH 6.5-7.5；Lactobacillus casei, bifidobacterium breve and the fermentation culture conditions of lactobacillus acidophilus are：28-37
DEG C, 50-100r/min fermentations cultivate 4-7d, to pH 4.5-6.0.
In the step (4), Lactobacillus casei, bifidobacterium breve and the inoculative proportion of lactobacillus acidophilus are 1.5-2:2-3:
1-3。
In the step (5), the centrifugal condition of aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution is：4 DEG C, 6000-8000r/
Min, centrifuges 5min；Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution press 3:1 mixing.
In the step (6), 30-40 parts of trehalose, 10-20 parts of xylose, 80-100 parts of plant extraction liquid are added.
Compared with prior art, the present invention has following excellent technique effect：
(1) beneficial micro- life such as Sphingol single-cell, Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus in the present invention
Thing, formaldehyde, benzene, dimethylbenzene, phenol in some active materials, the air ambient that can degrade etc. are produced by its body metabolism and is waved
Hair property aromatic hydrocarbon pollutant, suppresses the breeding of harmful bacteria in environment, so as to improve ambient air quality；Action effect persistently, can
Reach the purpose for removing volatile contaminant.
(2) plant extraction liquid in the present invention, pure natural nuisanceless, environmental protection contains the distinctive natural faint scent of plant
And nutritional ingredient, poisonous and hazardous material will not be produced, to environment without any damage.
(3) present invention can be used for removing in-car with the arene compound such as volatile formaldehyde and benzene, toluene, involved
Constituent be pure natural, without artificial chemistry synthetic ingredient, secondary pollution will not be caused to environment；Can effectively be degraded car
Interior escaping gas, without obvious peculiar smell, improves car indoor air quality and comfort.
Embodiment
The present invention is further explained with reference to embodiments：
Embodiment 1
A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, preparing the raw material of the probiotics includes following thing
Matter：Sphingomonas.5 parts of Sphingol single-cell, 1.5 parts of Lactobacillus casei Lactobacillus casei, short bifid bar
1 part of 2 parts of bacterium Bifidobacterium breve, lactobacillus acidophilus Lactobacillus acidophilus, culture medium into
Divide 60 parts, 30 parts of trehalose, 20 parts of xylose, 80 parts of plant extraction liquid, 2500 parts of distilled water.
A kind of preparation method for the probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, comprises the following steps：
(1) prepare：
Prepare oxygen liquid culture medium, aerobic solid medium, anaerobic liquid culture medium, anaerobic solids culture medium and plant
Extract solution.
Aerobic Liquid Culture based component is peptone 3.0g, beef extract 1g, NaCl 10g, distilled water 1000mL, and tune pH is
6.0-7.5,121 DEG C, sterilize 30min.
Aerobic solid culture based component is agar 15g, peptone 3.0g, beef extract 1g, NaCl 10g, distilled water
1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
Anaerobic liquid medium component is dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80
0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Anaerobic solids medium component is agar 15g, dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g,
Tween800.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Plant extraction liquid is that the one or more in lemon, banana, apple, pickled cucumbers, tomato are smashed, and adds distilled water and boils
0.5h is boiled, suction filtration after room temperature is cooled to, takes supernatant standby.
(2) activate：
By Sphingol single-cell strain in 37 DEG C of water-bath activation 1min, dilution spread is in aerobic solid medium, in 37 DEG C
Cultivate 24h；By Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus respectively at 37 DEG C of water-bath activation 1min, dilution respectively is applied
Anaerobic solids culture medium is distributed in, 24h is cultivated in 37 DEG C.
(3) it is inoculated with：
Picking Sphingol single-cell strain single bacterium colony is seeded to the purebred culture of aerobic fluid nutrient medium, in 37 DEG C, 80r/min
Cultivate to logarithmic phase；Picking Lactobacillus casei, bifidobacterium breve and Lactobacillus acidophilus species' single bacterium colony are seeded to detest respectively respectively
The purebred culture of oxygen liquid culture medium, is cultivated to logarithmic phase in 37 DEG C, 50r/min.
(4) fermented and cultured：
The Sphingol single-cell of logarithmic phase is linked into aerobic fluid nutrient medium in 3% ratio, seals, is passed through sterile
Air, ferments in 37 DEG C, 150r/min, 4d is cultivated, to pH 6.5；By the Lactobacillus casei of logarithmic phase, bifidobacterium breve and thermophilic
Lactobacillus lactis, is linked into anaerobic liquid culture medium in 3% ratio, wherein, Lactobacillus casei, bifidobacterium breve and acidophilus breast
The inoculative proportion of bacillus is 1.5:2:1, sealing is fermented in 37 DEG C, 80r/min, 4d is cultivated, to pH 5.0.
(5) centrifuge：
Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution after having fermented, respectively at 4 DEG C, 6000r/min centrifugation 5min, obtain supernatant
Liquid, by 3:1 mixing.
(6) it is filling：
Add 30 parts of trehalose, 20 parts of xylose, 80 parts of plant extraction liquid to be sufficiently mixed, working solution is made filling.
As shown in table 1, formaldehyde, aromatic hydrocarbon burst size are carried out to the automobile just fitted up to detect, under conditions of closed, passed through
The probiotics is sprayed, slightly humidity is defined by surface, the clearance rate that formaldehyde and aromatic hydrocarbon (benzene) are detected after 48h is
92.50%th, 93.40%, wherein this arene compound is using benzene as detection benchmark, and measured result meets in-car in one hour
Air quality, free from extraneous odour with fresh air, is effectively improved in-car air quality.
The formaldehyde of table 1, the detection of aromatic hydrocarbon burst size
Embodiment 2
A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, preparing the raw material of the probiotics includes following thing
Matter：Sphingomonas.3 parts of Sphingol single-cell, 2 parts of Lactobacillus casei Lactobacillus casei, bifidobacterium breve
1.5 parts of 2.5 parts of Bifidobacterium breve, lactobacillus acidophilus Lactobacillus acidophilus, culture medium
80 parts of composition, 40 parts of trehalose, 15 parts of xylose, 85 parts of plant extraction liquid, 2500 parts of distilled water.
A kind of preparation method for the probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, comprises the following steps：
(1) prepare：
Prepare oxygen liquid culture medium, aerobic solid medium, anaerobic liquid culture medium, anaerobic solids culture medium and plant
Extract solution.
Aerobic Liquid Culture based component is peptone 3.0g, beef extract 1g, NaCl 10g, distilled water 1000mL, and tune pH is
6.0-7.5,121 DEG C, sterilize 30min.
Aerobic solid culture based component is agar 15g, peptone 3.0g, beef extract 1g, NaCl 10g, distilled water
1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
Anaerobic liquid medium component is dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80
0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Anaerobic solids medium component is agar 15g, dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g,
Tween80 0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Plant extraction liquid is that the one or more in lemon, banana, apple, pickled cucumbers, tomato are smashed, and adds distilled water and boils
0.5h is boiled, suction filtration after room temperature is cooled to, takes supernatant standby.
(2) activate：
By Sphingol single-cell strain in 37 DEG C of water-bath activation 1min, dilution spread is in aerobic solid medium, in 30 DEG C
Cultivate 24h；By Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus respectively at 37 DEG C of water-bath activation 1min, dilution respectively is applied
Anaerobic solids culture medium is distributed in, 24h is cultivated in 37 DEG C.
(3) it is inoculated with：
Picking Sphingol single-cell strain single bacterium colony is seeded to the purebred culture of aerobic fluid nutrient medium, in 30 DEG C, 80r/min
Cultivate to logarithmic phase；Picking Lactobacillus casei, bifidobacterium breve and Lactobacillus acidophilus species' single bacterium colony are seeded to detest respectively respectively
The purebred culture of oxygen liquid culture medium, is cultivated to logarithmic phase in 37 DEG C, 60r/min.
(4) fermented and cultured：
The Sphingol single-cell of logarithmic phase is linked into aerobic fluid nutrient medium in 1.5% ratio, seals, is passed through nothing
Bacterium air, ferments in 30 DEG C, 120r/min, 5d is cultivated, to pH 6.8；By the Lactobacillus casei of logarithmic phase, bifidobacterium breve and
Lactobacillus acidophilus, is linked into anaerobic liquid culture medium in 2% ratio, wherein, Lactobacillus casei, bifidobacterium breve and acidophilus
The inoculative proportion of lactobacillus is 2:2.5:1.5, sealing is fermented in 37 DEG C, 80r/min, 4d is cultivated, to pH 4.8.
(5) centrifuge：
Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution after having fermented, respectively at 4 DEG C, 6000r/min centrifugation 5min, obtain supernatant
Liquid, by 3:1 mixing.
(6) it is filling：
Add 40 parts of trehalose, 15 parts of xylose, 85 parts of plant extraction liquid to be sufficiently mixed, working solution is made filling.
As shown in table 2, formaldehyde, aromatic hydrocarbon burst size are carried out to the automobile for fitting up half a year to detect, under conditions of closed, led to
Cross and spray the probiotics, slightly humidity is defined by surface, the clearance rate that formaldehyde and aromatic hydrocarbon (toluene) are detected after 48h is
89.60%th, 78.56%, wherein this arene compound is using toluene as detection benchmark, and measured result meets car in one hour
Interior air quality.Free from extraneous odour with fresh air, is effectively improved in-car air quality after processing.
The formaldehyde of table 2, the detection of aromatic hydrocarbon burst size
Embodiment 3
A kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, preparing the raw material of the probiotics includes following thing
Matter：Sphingomonas.5 parts of Sphingol single-cell, 1.5 parts of Lactobacillus casei Lactobacillus casei, short bifid bar
1.5 parts of 2 parts of bacterium Bifidobacterium breve, lactobacillus acidophilus Lactobacillus acidophilus, culture medium
60 parts of composition, 35 parts of trehalose, 15 parts of xylose, 80 parts of plant extraction liquid, 2500 parts of distilled water.
A kind of preparation method for the probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, comprises the following steps：
(1) prepare：
Prepare oxygen liquid culture medium, aerobic solid medium, anaerobic liquid culture medium, anaerobic solids culture medium and plant
Extract solution.
Aerobic Liquid Culture based component is peptone 3.0g, beef extract 1g, NaCl 10g, distilled water 1000mL, and tune pH is
6.0-7.5,121 DEG C, sterilize 30min.
Aerobic solid culture based component is agar 15g, peptone 3.0g, beef extract 1g, NaCl 10g, distilled water
1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
Anaerobic liquid medium component is dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80
0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Anaerobic solids medium component is agar 15g, dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g,
Tween80 0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
Plant extraction liquid is that the one or more in lemon, banana, apple, pickled cucumbers, tomato are smashed, and adds distilled water and boils
0.5h is boiled, suction filtration after room temperature is cooled to, takes supernatant standby.
(2) activate：
By Sphingol single-cell strain in 37 DEG C of water-bath activation 1.5min, dilution spread is in aerobic solid medium, in 37
DEG C culture 24h；By Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus respectively at 37 DEG C of water-bath activation 1.5min, difference is dilute
Release and be coated on anaerobic solids culture medium, 24h is cultivated in 37 DEG C.
(3) it is inoculated with：
Picking Sphingol single-cell strain single bacterium colony is seeded to the purebred culture of aerobic fluid nutrient medium, in 37 DEG C, 60r/min
Cultivate to logarithmic phase；Picking Lactobacillus casei, bifidobacterium breve and Lactobacillus acidophilus species' single bacterium colony are seeded to detest respectively respectively
The purebred culture of oxygen liquid culture medium, is cultivated to logarithmic phase in 37 DEG C, 80r/min.
(4) fermented and cultured：
The Sphingol single-cell of logarithmic phase is linked into aerobic fluid nutrient medium in 3% ratio, seals, is passed through sterile
Air, ferments in 37 DEG C, 150r/min, 4d is cultivated, to pH 7.2；By the Lactobacillus casei of logarithmic phase, bifidobacterium breve and thermophilic
Lactobacillus lactis, is linked into anaerobic liquid culture medium in 2% ratio, wherein, Lactobacillus casei, bifidobacterium breve and acidophilus breast
The inoculative proportion of bacillus is 2:1.5:1.5, sealing is fermented in 37 DEG C, 80r/min, 4d is cultivated, to pH 5.0.
(5) centrifuge：
Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution after having fermented, respectively at 4 DEG C, 6000r/min centrifugation 5min, obtain supernatant
Liquid, by 3:1 mixing.
(6) it is filling：
Add 35 parts of trehalose, 15 parts of xylose, 80 parts of plant extraction liquid to be sufficiently mixed, working solution is made filling.
As shown in table 3, PARA FORMALDEHYDE PRILLS(91,95), aromatic hydrocarbon burst size distinguish exceeded twice of automobile progress formaldehyde, aromatic hydrocarbon burst size
Detection, under conditions of closed, the probiotics is sprayed by air humidifier, slightly humidity is defined by surface, is detected after 24h
To formaldehyde and aromatic hydrocarbon (toluene) clearance rate be 86.60%, 80.76%, this aromatic hydrocarbon using toluene as detection project benchmark,
Measured result meets average in one hour.The free from extraneous odour with fresh air after processing, repeatedly matches somebody with somebody and is effectively improved in-car air matter after applying
Amount, reaches indoor air quality standard.
The formaldehyde of table 3, the detection of aromatic hydrocarbon burst size
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (14)
1. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon, it is characterised in that prepare the raw material of the probiotics
Including following material：Sphingomonas.3-5 parts of Sphingol single-cell, Lactobacillus casei Lactobacillus casei 1-3
Part, 1-3 parts of bifidobacterium breve Bifidobacterium breve, lactobacillus acidophilus Lactobacillus acidophilus
1-3 parts, 40-80 parts of medium component, 30-40 parts of trehalose, 10-20 parts of xylose, 80-100 parts of plant extraction liquid, distilled water
2500-3000 parts.
2. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 1, it is characterised in that described
Medium component includes aerobic fluid nutrient medium, aerobic solid medium, anaerobic liquid culture medium and anaerobic solids culture medium.
3. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 1 or 2, it is characterised in that
The Sphingol single-cell be aerobic fermentation, aerobic Liquid Culture based component be peptone 3.0g, beef extract 1g, NaCl 10g,
Distilled water 1000mL, tune pH are 6.0-7.5, and 121 DEG C, sterilize 30min.
4. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 3, it is characterised in that described
Aerobic solid culture based component be agar 15g, peptone 3.0g, beef extract 1g, NaCl 10g, distilled water 1000mL, adjust pH be
6.0-7.5,121 DEG C, sterilize 30min.
5. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 1 or 2, it is characterised in that
The Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus are anaerobic fermentation, and anaerobic liquid medium component is dusty yeast
1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80 0.1mL, 5% bean cake powder leachate 80mL, distilled water
1000mL, 115 DEG C, sterilize 20min.
6. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 5, it is characterised in that described
Anaerobic solids medium component is agar 15g, dusty yeast 1.5g, lactose 1g, glucose 1g, K2HPO40.2g, Tween80
0.1mL, 5% bean cake powder leachate 80mL, distilled water 1000mL, 115 DEG C, sterilize 20min.
7. a kind of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 1, it is characterised in that described
Plant extraction liquid is that the one or more in lemon, banana, apple, pickled cucumbers, tomato are smashed, and adds distilled water and boils 0.5h,
Suction filtration after room temperature is cooled to, takes supernatant standby.
8. a kind of preparation side of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon as described in claim 1-7 any one
Method, it is characterised in that comprise the following steps：
(1) prepare：Oxygen liquid culture medium, aerobic solid medium, anaerobic liquid culture are prepared as described in claim 3-7
Base, anaerobic solids culture medium and plant extraction liquid；
(2) activate：By Sphingol single-cell strain water-bath activation, dilution spread is in aerobic solid medium culture；By cheese breast
Bacillus, bifidobacterium breve and Lactobacillus acidophilus species' difference water-bath activation, dilution spread is in anaerobic solids medium culture respectively；
(3) it is inoculated with：Picking Sphingol single-cell strain single bacterium colony is seeded to the purebred culture of aerobic fluid nutrient medium, culture to logarithm
Phase；Picking Lactobacillus casei, bifidobacterium breve and Lactobacillus acidophilus species' single bacterium colony are seeded to anaerobic liquid culture respectively respectively
The purebred culture of base, culture to logarithmic phase；
(4) fermented and cultured：The Sphingol single-cell of logarithmic phase is linked into aerobic fluid nutrient medium in 1%-5% ratio,
Sealing, is passed through filtrated air, fermented and cultured；By the Lactobacillus casei, bifidobacterium breve and lactobacillus acidophilus of logarithmic phase, by 1%-
5% ratio is linked into anaerobic liquid culture medium, sealing, fermented and cultured；
(5) centrifuge：Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution after having fermented, centrifuge, obtain supernatant respectively, mixing；
(6) it is filling：Add trehalose, xylose, plant extraction liquid to be sufficiently mixed, working solution is made filling.
9. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8, it is special
Levy and be, in the step (2), the water-bath activation condition of Sphingol single-cell is：37 DEG C, 1-2min is activated, condition of culture is：
28-37 DEG C, cultivate 24h；Lactobacillus casei, bifidobacterium breve and the water-bath of lactobacillus acidophilus activation condition are：37 DEG C, activate 1-
2min, condition of culture is：28-37 DEG C, cultivate 24h.
10. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8, it is special
Levy and be, in the step (3), the purebred condition of culture of Sphingol single-cell is：28-37 DEG C, 50-80r/min；Cheese breast bar
The purebred condition of culture of bacterium, bifidobacterium breve and lactobacillus acidophilus is：28-37 DEG C, 50-80r/min.
11. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8, it is special
Levy and be, in the step (4), the fermentation culture conditions of Sphingol single-cell are：28-37 DEG C, 150-180r/min fermentations, training
4-7d is supported, to pH 6.5-7.5；Lactobacillus casei, bifidobacterium breve and the fermentation culture conditions of lactobacillus acidophilus are：28-37
DEG C, 50-100r/min fermentations cultivate 4-7d, to pH 4.5-6.0.
12. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8 or 11,
Characterized in that, in the step (4), Lactobacillus casei, bifidobacterium breve and the inoculative proportion of lactobacillus acidophilus are 1.5-2:
2-3:1-3。
13. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8, it is special
Levy and be：In the step (5), the centrifugal condition of aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution is：4 DEG C, 6000-8000r/min,
Centrifuge 5min；Aerobic bacteria bacterium solution and anaerobic bacteria bacterium solution press 3:1 mixing.
14. a kind of preparation method of probiotics for dispelling in-car formaldehyde and aromatic hydrocarbon according to claim 8, it is special
Levy and be：In the step (6), 30-40 parts of trehalose, 10-20 parts of xylose, 80-100 parts of plant extraction liquid are added.
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