CN108949381A - A kind of medical instrument multienzyme cleaning agent - Google Patents
A kind of medical instrument multienzyme cleaning agent Download PDFInfo
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- CN108949381A CN108949381A CN201811046808.8A CN201811046808A CN108949381A CN 108949381 A CN108949381 A CN 108949381A CN 201811046808 A CN201811046808 A CN 201811046808A CN 108949381 A CN108949381 A CN 108949381A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/22—Carbohydrates or derivatives thereof
- C11D3/222—Natural or synthetic polysaccharides, e.g. cellulose, starch, gum, alginic acid or cyclodextrin
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11D3/36—Organic compounds containing phosphorus
- C11D3/364—Organic compounds containing phosphorus containing nitrogen
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/37—Polymers
- C11D3/3703—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C11D3/3707—Polyethers, e.g. polyalkyleneoxides
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/722—Ethers of polyoxyalkylene glycols having mixed oxyalkylene groups; Polyalkoxylated fatty alcohols or polyalkoxylated alkylaryl alcohols with mixed oxyalkylele groups
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
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Abstract
The invention discloses a kind of medical instrument multienzyme cleaning agents, belong to field of medical technology.The present invention be added 4- formylphenyl boronic acid in storage can effective protease inhibition vigor, prevent the reduction of enzyme activity；With beta-cyclodextrin in aqueous solution and nonionic surfactant Pluronic-F88 by Host-guest Recognition, adjacent beta-cyclodextrin then passes through cavity periphery hydroxyl and forms hydrogen bond the present invention, will not influence the performance of other cleaning active agent functions；With high containing hydrogen silicone oil, hexamethyldisiloxane etc. for raw material, the tertiary amine groups and sodium chloroacetate in intermediate prevent from carrying out secondary pollution to equipment surfaces the present invention through quaternization reaction；Scutelloside and Hemarisin is added in the present invention, reduce due to microbial load it is excessively high caused by rejection and pyrogen reaction, increase the safety in utilization of medical instrument.The present invention solves the other biological enzyme in current multienzyme cleaning agent in protease energy decomposing solution, causes the bioenzyme activity in enzyme cleaning agent low, the problem of cleaning effect difference.
Description
Technical field
The invention belongs to field of medical technology, and in particular to a kind of medical instrument multienzyme cleaning agent.
Background technique
In recent years, for the generation for reducing inside-hospital infection, the cleaning for polluting medical instrument is increasingly taken seriously.It is many expensive
There is the accurate medical instrument of weight tiny hollow lacuna to be infected with many mucus secretions, blood etc., commonly into after human body
Biological cleaner tend not to remove.In addition, the substances such as mucus secretion, blood are easily adsorbed in the lacunas such as endoscope
Wall prevents disinfectant from substantially arriving at, in turn results in disinfection failure.After use, surface often remains bloodstain, oil to surgical instrument
The organic pollutants such as adipose tissue's liquid.And surgical instrument will lead to the accumulation for bringing biomembrane, biomembrane tool in Reusability
There is very big harmfulness, it is estimated that 65% human body bacterium infection is all related to biomembrane.After biofilm formation, 2400 times are needed
Antibiotic dosage, lethality is up to 50% ~ 60%.If relying on the modes such as disinfection/sterilizing merely kills the bacterium in biomembrane
Be it is far from being enough, only thoroughly remove biomembrane can just be effectively reduced infection risk.There is no the cleaning of high quality just not
Qualified sterilizing is had, therefore, bactericidal effect when cleaning is to guarantee that later period sterilizing is successfully crucial.The main function of cleaning is
Remove article surface be greater than 95% dirt and biological load, reduce to the maximum extent due to microbial load it is excessively high caused by row
Different and pyrogen reaction, therefore the cleaning to medical instrument are intended to reach clean standard every time.If these biomembranes and bloodstain
Equal dirts cleaning is not thorough, and will lead to dirt remains in equipment surfaces, can also be substantially damaged instrument, and that shortens instrument uses the longevity
Life.
It is noted that general cleaning method is difficult to be thoroughly removed biomembrane.It preferably goes on the market at present
Except Biofilm Environment is to use enzyme cleaning solution when surgical instrument is cleaned.It, which can effectively be decomposed, is infected on the medical instrument
Various secretion, blood, including protein, mucopolysaccharide, lipopolysaccharides etc., it is corrosion-free to medical instrument and degradable, not to environment
It pollutes.But since the regulation of medical cleaning agent is not perfect, more or less all there are some problems in the enzyme of many producers,
For example foam is excessively high that automatic occasion of rinsing device is caused to be alarmed；Detergency does not cause dirt to remain in equipment surfaces by force；Only use one
Kind enzyme causes removal biomembrane to be not thorough；It is rinsability it is poor cause cleaning agent part to remain in equipment surfaces, lead to equipment surfaces
Obfuscation, it is possible to bring human body into the use residual cleaner of surgical instrument and cause rejection.
A kind of concentration multienzyme medical cleaning agent is disclosed at present, and the protease in enzyme cleaning agent is with other biological enzyme same
In solution, due to the other biological enzyme in protease energy decomposing solution, cause the bioenzyme activity in enzyme cleaning agent low, enzyme cleaning
The cleaning effect of agent is poor, and the sterilizing ability of enzyme cleaning agent is weak.Therefore a kind of efficient medical instrument multienzyme cleaning need to be developed
Agent.
Summary of the invention
The technical problems to be solved by the invention: for other in current multienzyme cleaning agent in protease energy decomposing solution
Biological enzyme causes the bioenzyme activity in enzyme cleaning agent low, the problem of cleaning effect difference, provides a kind of medical instrument multienzyme cleaning
Agent.
In order to solve the above technical problems, the present invention is using technical solution as described below:
A kind of medical instrument multienzyme cleaning agent, including following component, 4 ~ 7 parts of lecithin, 10 ~ 20 parts of Polyethylene glycol-2000s, 80 ~
100 parts of sterile waters, further include 10 ~ 20 parts of activated basic albumen enzyme additives, 5 ~ 9 parts of surfactants, 2 ~ 6 parts go heat source to add
Agent, 5 ~ 10 parts of auxiliary enzyme additives.
The activated basic albumen enzyme additive the preparation method comprises the following steps:
(1) according to the mass fraction, 3 ~ 6 parts of calcium chloride, 0.5 ~ 0.8 part of 4- formylphenyl boronic acid, 1 ~ 3 part of propylene glycol monomethyl ether, 5 are taken
~ 10 parts of glycerine, 3 ~ 8 parts of alkali proteases are stirred, and obtain stirring mixture, take Pluronic-F88 1 ~ 3:20 in mass ratio
~ 30 are added distilled water mixing, obtain mixed liquor a, take beta-cyclodextrin 1 ~ 3:90 in mass ratio ~ 100 that distilled water mixing is added, must mix
Liquid b；
(2) it takes stirring mixture 2 ~ 5:80 in mass ratio ~ 100 that mixed liquor b is added, is stirred in 20 ~ 25 DEG C, then stirring is added dropwise
The mixed liquor a of mixture quality 70 ~ 80% controls 30 ~ 50min of time for adding, keeps the temperature in 25 ~ 30 DEG C, and centrifugation takes pellet frozen
Drying is to get activated basic albumen enzyme additive.
Stirring mixture is stirred condition in the step (1) are as follows: is stirred 30 ~ 50min in 25 ~ 30 DEG C.
The surfactant the preparation method comprises the following steps:
S1. according to the mass fraction, take 3 ~ 8 parts of octamethylcy-clotetrasiloxanes, 2 ~ 6 parts of hexamethyldisiloxane, 10 ~ 20 parts of height hydrogeneous
Silicone oil is passed through nitrogen protection, is stirred, and must stir mixture a, takes stirring mixture a 40 ~ 50:1 in mass ratio ~ 3 that sulphur is added
Acid keeps the temperature in 65 ~ 75 DEG C, is cooled to room temperature, obtains cooled material, takes cooled material 10 ~ 20:3 in mass ratio ~ 5 that sodium bicarbonate is added and stirs
Mixing is mixed, is filtered, is taken filtrate decompression to distill, obtain vacuum distillation object；
S2. N is taken, vacuum distillation object is added in N- dimethylallylamine 3 ~ 5:8 in mass ratio ~ 10, it is passed through nitrogen protection, then plus
Entering to be evaporated under reduced pressure 1,3- divinyl -1,1 of amount of substance 3 ~ 5%, 3,3- tetramethyl disiloxane platinum are kept the temperature in 110 ~ 120 DEG C,
Vacuum distillation, obtains vacuum distillation object, takes vacuum distillation object 3 ~ 6:8 in mass ratio ~ 10 that isopropanol is added, is passed through nitrogen protection, drips
Plus-minus pressure distillation amount of substance 30 ~ 40% sodium chloroacetate be stirred, rotary evaporation, take rotary evaporation object 1 ~ 3:7 in mass ratio ~
10 are added acetone, keep the temperature in 2 ~ 5 DEG C, take precipitating dry to get surfactant.
That mixture a is stirred in the step S1 is stirred condition are as follows: is stirred 20 ~ 30min in 60 ~ 80 DEG C.
The source additive that reduces phlegm and internal heat are as follows: take scutelloside 2 ~ 5:1 in mass ratio ~ 3 be added Hemarisin mixing to get.
The auxiliary enzyme additive are as follows: take lysozyme, amylase 6 ~ 9:2 in mass ratio ~ 5:2 ~ 6 that lipase mixing is added,
To obtain the final product.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention be added 4- formylphenyl boronic acid in storage can effective protease inhibition vigor, prevent protease itself water
Solution or by other enzyme hydrolysis, it is dilute with enzyme preparation when in use, enzyme activity again can fast quick-recovery, protection is played to enzyme activity and is made
With propylene glycol monomethyl ether, which is added, can play the other materials that auxiliary dissolves with prevents liquid of protease from contacting to multienzyme cleaning, and press down
The formation of protomere is made or avoids, the ability that glycerine formation hydrogen bond is added is smaller than water, breaks to protein molecule hydrogen bond structure
Bad power is also smaller, water as solvent can be replaced to have stabilization to protease, damage of the mating reaction reduction to enzyme is formed, to multienzyme
Cleaning active object forms protection, prevents the reduction of enzyme activity；
(2) present invention passes through Host-guest Recognition with beta-cyclodextrin in aqueous solution and nonionic surfactant Pluronic-F88,
Under the hydrophobic forces, hydrogen bond the effects of, hydrophobic chain PPO is entered in the hydrophobic cavity of beta-cyclodextrin, forms building oversubscription
The basic unit of sub- tiny balloon, the hydrophilic chain PEO at the both ends Pluronic-F88 become flexible chain in aqueous solution, and centre is dredged
Water chain PPO is rigidity or stiff chain, and Pluronic-F88 has stronger self aggregation or self-assembly ability, enters beta-cyclodextrin sky
The part hydrophobic chain PPO of chamber will act as a hard end, become the axle center of Supramolecular self assembly, hydrophilic chain PEO as soft end, into
Row Supramolecular self assembly, adjacent beta-cyclodextrin then pass through cavity periphery hydroxyl and form hydrogen bond, link together, alkali protease meeting
It is spontaneous to enter in tiny balloon, it is wrapped up, to be separated with other enzymes in cleaning agent, will not influence other cleanings and live
The performance of property agent function；
(3) present invention is urged with high containing hydrogen silicone oil, octamethylcy-clotetrasiloxane, hexamethyldisiloxane etc. for raw material in the concentrated sulfuric acid
It is reacted under change effect, adds N, N- dimethylallylamine carries out hydrosilylation under catalytic action, obtains
Mesosome, tertiary amine groups and sodium chloroacetate in intermediate obtain surfactant, contain carboxyl, sulfo group etc. through quaternization reaction
Anionic hydrophilic group, it may have quaternary ammonium cationic groups, the characteristic with amphoteric surfactant, surfactant obtained
The presence of middle carboxybetaine can assign its excellent anti-protein absorption property, and siloxane main chain can assign its good physiology
Inertia and it is nontoxic the features such as, there is it in cleaning preferable water-soluble, surface tension can be effectively reduced, so that some albumen
Matter molecule is removed from medical instrument, and hydrone will form one layer of fine and close water molecule layer in hydrophilic surface after cleaning, thus
Barrier action is played, so that impedance protein molecule is adsorbed in medical apparatus surface redeposition, prevents the protein material after washing
Secondary pollution is carried out Deng to equipment surfaces；
(4) scutelloside and Hemarisin is added in the present invention, has the function of preferable anti-microbial property and antiendotoxin, scutelloside
With preferable antibacterial action, and Hemarisin energy activating macrophage generates the expression of anti -inflammatory cytokine, and the two is assisted
Same-action prevents the residual of some bacteriums after cleaning, and preventing from entering will cause wound infection after human body and cause inflammation, reduce due to
Rejection and pyrogen reaction, increase the safety in utilization of medical instrument caused by microbial load is excessively high.
Specific embodiment
Pluronic-F88:PEO133-PPO39-PEO133, polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer.
Activated basic albumen enzyme additive the preparation method comprises the following steps:
(1) according to the mass fraction, 3 ~ 6 parts of calcium chloride, 0.5 ~ 0.8 part of 4- formylphenyl boronic acid, 1 ~ 3 part of propylene glycol monomethyl ether, 5 are taken
~ 10 parts of glycerine, 3 ~ 8 parts of alkali proteases, are stirred 30 ~ 50min in 25 ~ 30 DEG C, obtain stirring mixture, take
Distilled water mixing is added in Pluronic-F88 1 ~ 3:20 in mass ratio ~ 30, obtains mixed liquor a, takes beta-cyclodextrin in mass ratio 1 ~ 3:
90 ~ 100 are added distilled water mixing, obtain mixed liquor b；
(2) it takes stirring mixture 2 ~ 5:80 in mass ratio ~ 100 that mixed liquor b is added, is stirred 30 ~ 50min in 20 ~ 25 DEG C, then
Dropwise addition is stirred the mixed liquor a of amount of substance 70 ~ 80%, controls 30 ~ 50min of time for adding, in 25 ~ 30 DEG C of 1 ~ 3h of heat preservation, from
The heart takes pellet frozen dry to get activated basic albumen enzyme additive.
Surfactant the preparation method comprises the following steps:
S1. according to the mass fraction, take 3 ~ 8 parts of octamethylcy-clotetrasiloxanes, 2 ~ 6 parts of hexamethyldisiloxane, 10 ~ 20 parts of height hydrogeneous
Silicone oil is passed through nitrogen protection, is stirred 20 ~ 30min in 60 ~ 80 DEG C, must stir mixture a, takes stirring mixture a by quality
The sulfuric acid that mass fraction is 98% is added than 40 ~ 50:1 ~ 3 to be cooled to room temperature in 65 ~ 75 DEG C of 6 ~ 9h of heat preservation, obtain cooled material, take cold
But sodium bicarbonate is added in object 10 ~ 20:3 in mass ratio ~ 5, is stirred 20 ~ 50min, filters, takes filtrate decompression to distill, must depressurize
Distillation；
S2. N is taken, vacuum distillation object is added in N- dimethylallylamine 3 ~ 5:8 in mass ratio ~ 10, it is passed through nitrogen protection, then plus
Enter to be evaporated under reduced pressure 1,3- divinyl -1,1 of amount of substance 3 ~ 5%, 3,3- tetramethyl disiloxane platinum, keep the temperature 3 in 110 ~ 120 DEG C ~
5h, vacuum distillation, obtains vacuum distillation object, takes vacuum distillation object 3 ~ 6:8 in mass ratio ~ 10 that isopropanol is added, is passed through nitrogen protection,
The sodium chloroacetate of vacuum distillation amount of substance 30 ~ 40% is added dropwise, is stirred 2 ~ 5h, rotary evaporation takes rotary evaporation object in mass ratio
Acetone is added in 1 ~ 3:7 ~ 10, in 2 ~ 5 DEG C of 12 ~ 18h of heat preservation, takes precipitating dry to get surfactant.
Reduce phlegm and internal heat source additive: take scutelloside 2 ~ 5:1 in mass ratio ~ 3 be added Hemarisin mixing to get.
Auxiliary enzyme additive: take lysozyme, amylase 6 ~ 9:2 in mass ratio ~ 5:2 ~ 6 be added lipase mixing to get.
A kind of medical instrument multienzyme cleaning agent, according to the mass fraction, including 10 ~ 20 parts of activated basic albumen enzyme additives, 5
~ 9 parts of surfactants, 2 ~ 6 parts of source additives that reduce phlegm and internal heat, 5 ~ 10 parts of auxiliary enzyme additives, 4 ~ 7 parts of lecithin, 10 ~ 20 parts of poly- second two
Alcohol -2000,80 ~ 100 parts of sterile waters.
Pluronic-F88:PEO133-PPO39-PEO133, polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer.
Activated basic albumen enzyme additive the preparation method comprises the following steps:
(1) according to the mass fraction, 3 parts of calcium chloride, 0.5 part of 4- formylphenyl boronic acid, 1 part of propylene glycol monomethyl ether, 5 parts the third three are taken
Alcohol, 3 parts of alkali proteases, are stirred 30min in 25 DEG C, obtain stirring mixture, take Pluronic-F88 1:20 in mass ratio
Distilled water mixing is added, obtains mixed liquor a, takes beta-cyclodextrin 1:90 in mass ratio that distilled water mixing is added, obtains mixed liquor b；
(2) it takes stirring mixture 2:80 in mass ratio that mixed liquor b is added, is stirred 30min in 20 DEG C, then be added dropwise and be stirred
The mixed liquor a of amount of substance 70% controls time for adding 30min, and in 25 DEG C of heat preservation 1h, centrifugation takes pellet frozen dry to get work
Property basic protein enzyme additive.
Surfactant the preparation method comprises the following steps:
S1. according to the mass fraction, 3 parts of octamethylcy-clotetrasiloxanes, 2 parts of hexamethyldisiloxane, 10 parts of high containing hydrogen silicone oils are taken, are led to
Enter nitrogen protection, be stirred 20min in 60 DEG C, mixture a must be stirred, takes stirring mixture a 40:1 in mass ratio that matter is added
The sulfuric acid that amount score is 98% is cooled to room temperature in 65 DEG C of heat preservation 6h, obtains cooled material, takes cooled material 10:3 in mass ratio that carbon is added
Sour hydrogen sodium, is stirred 20min, and filtering takes filtrate decompression to distill, obtains vacuum distillation object；
S2. it takes N, N- dimethylallylamine 3:8 in mass ratio that vacuum distillation object is added, is passed through nitrogen protection, adds decompression
1,3- divinyl -1,1 of amount of substance 3% is distilled, 3,3- tetramethyl disiloxane platinum, in 110 DEG C of heat preservation 3h, vacuum distillation is obtained
It is evaporated under reduced pressure object, takes vacuum distillation object 3:8 in mass ratio that isopropanol is added, is passed through nitrogen protection, vacuum distillation amount of substance is added dropwise
30% sodium chloroacetate is stirred 2h, and rotary evaporation takes rotary evaporation object 1:7 in mass ratio that acetone is added, and keeps the temperature in 2 DEG C
12h takes precipitating dry to get surfactant.
Reduce phlegm and internal heat source additive: take scutelloside 2:1 in mass ratio be added Hemarisin mixing to get.
Auxiliary enzyme additive: take lysozyme, amylase 6:2:2 in mass ratio be added lipase mixing to get.
A kind of medical instrument multienzyme cleaning agent, according to the mass fraction, including 10 parts of activated basic albumen enzyme additives, 5 parts
Surfactant, 2 parts of source additives that reduce phlegm and internal heat, 5 parts of auxiliary enzyme additives, 4 parts of lecithin, 10 parts of Polyethylene glycol-2000s, 80 parts of nothings
Bacterium water.
Pluronic-F88:PEO133-PPO39-PEO133, polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer.
Activated basic albumen enzyme additive the preparation method comprises the following steps:
(1) according to the mass fraction, 5 parts of calcium chloride, 0.6 part of 4- formylphenyl boronic acid, 2 parts of propylene glycol monomethyl ethers, 8 parts the third three are taken
Alcohol, 6 parts of alkali proteases, are stirred 40min in 28 DEG C, obtain stirring mixture, take Pluronic-F88 2:25 in mass ratio
Distilled water mixing is added, obtains mixed liquor a, takes beta-cyclodextrin 2:95 in mass ratio that distilled water mixing is added, obtains mixed liquor b；
(2) it takes stirring mixture 4:90 in mass ratio that mixed liquor b is added, is stirred 40min in 22 DEG C, then be added dropwise and be stirred
The mixed liquor a of amount of substance 75% controls time for adding 40min, and in 28 DEG C of heat preservation 2h, centrifugation takes pellet frozen dry to get work
Property basic protein enzyme additive.
Surfactant the preparation method comprises the following steps:
S1. according to the mass fraction, 6 parts of octamethylcy-clotetrasiloxanes, 4 parts of hexamethyldisiloxane, 15 parts of high containing hydrogen silicone oils are taken, are led to
Enter nitrogen protection, be stirred 25min in 70 DEG C, mixture a must be stirred, takes stirring mixture a 45:2 in mass ratio that matter is added
The sulfuric acid that amount score is 98% is cooled to room temperature in 70 DEG C of heat preservation 6h, obtains cooled material, takes cooled material 15:4 in mass ratio that carbon is added
Sour hydrogen sodium, is stirred 40min, and filtering takes filtrate decompression to distill, obtains vacuum distillation object；
S2. it takes N, N- dimethylallylamine 4:9 in mass ratio that vacuum distillation object is added, is passed through nitrogen protection, adds decompression
1,3- divinyl -1,1 of amount of substance 4% is distilled, 3,3- tetramethyl disiloxane platinum, in 115 DEG C of heat preservation 4h, vacuum distillation is obtained
It is evaporated under reduced pressure object, takes vacuum distillation object 5:9 in mass ratio that isopropanol is added, is passed through nitrogen protection, vacuum distillation amount of substance is added dropwise
35% sodium chloroacetate is stirred 4h, and rotary evaporation takes rotary evaporation object 2:8 in mass ratio that acetone is added, and keeps the temperature in 4 DEG C
16h takes precipitating dry to get surfactant.
Reduce phlegm and internal heat source additive: take scutelloside 4:2 in mass ratio be added Hemarisin mixing to get.
Auxiliary enzyme additive: take lysozyme, amylase 8:4:5 in mass ratio be added lipase mixing to get.
A kind of medical instrument multienzyme cleaning agent, according to the mass fraction, including 15 parts of activated basic albumen enzyme additives, 7 parts
Surfactant, 4 parts of source additives that reduce phlegm and internal heat, 8 parts of auxiliary enzyme additives, 5 parts of lecithin, 15 parts of Polyethylene glycol-2000s, 90 parts of nothings
Bacterium water.
Pluronic-F88:PEO133-PPO39-PEO133, polyoxyethylene-poly-oxypropylene polyoxyethylene block copolymer.
Activated basic albumen enzyme additive the preparation method comprises the following steps:
(1) according to the mass fraction, 6 parts of calcium chloride, 0.8 part of 4- formylphenyl boronic acid, 3 parts of propylene glycol monomethyl ethers, 10 parts the third three are taken
Alcohol, 8 parts of alkali proteases, are stirred 50min in 30 DEG C, obtain stirring mixture, take Pluronic-F88 3:30 in mass ratio
Distilled water mixing is added, obtains mixed liquor a, takes beta-cyclodextrin 3:100 in mass ratio that distilled water mixing is added, obtains mixed liquor b；
(2) it takes stirring mixture 5:100 in mass ratio that mixed liquor b is added, is stirred 50min in 25 DEG C, then stirring is added dropwise and mixes
Close the mixed liquor a of amount of substance 80%, control time for adding 50min, in 30 DEG C of heat preservation 3h, centrifugation, take pellet frozen to dry to get
Activated basic albumen enzyme additive.
Surfactant the preparation method comprises the following steps:
S1. according to the mass fraction, 8 parts of octamethylcy-clotetrasiloxanes, 6 parts of hexamethyldisiloxane, 20 parts of high containing hydrogen silicone oils are taken, are led to
Enter nitrogen protection, be stirred 30min in 80 DEG C, mixture a must be stirred, takes stirring mixture a 50:3 in mass ratio that matter is added
The sulfuric acid that amount score is 98% is cooled to room temperature in 75 DEG C of heat preservation 9h, obtains cooled material, takes cooled material 20:5 in mass ratio that carbon is added
Sour hydrogen sodium, is stirred 50min, and filtering takes filtrate decompression to distill, obtains vacuum distillation object；
S2. it takes N, N- dimethylallylamine 5:10 in mass ratio that vacuum distillation object is added, is passed through nitrogen protection, adds and subtract
1,3- divinyl -1,1 of pressure distillation amount of substance 5%, 3,3- tetramethyl disiloxane platinum are evaporated under reduced pressure in 120 DEG C of heat preservation 5h,
It must be evaporated under reduced pressure object, take vacuum distillation object 6:10 in mass ratio that isopropanol is added, be passed through nitrogen protection, vacuum distillation substance is added dropwise
The sodium chloroacetate of amount 40% is stirred 5h, and rotary evaporation takes rotary evaporation object 3:10 in mass ratio that acetone is added, and protects in 5 DEG C
Warm 18h takes precipitating dry to get surfactant.
Reduce phlegm and internal heat source additive: take scutelloside 5:3 in mass ratio be added Hemarisin mixing to get.
Auxiliary enzyme additive: take lysozyme, amylase 9:5:6 in mass ratio be added lipase mixing to get.
A kind of medical instrument multienzyme cleaning agent, according to the mass fraction, including 20 parts of activated basic albumen enzyme additives, 9 parts
Surfactant, 6 parts of source additives that reduce phlegm and internal heat, 10 parts of auxiliary enzyme additives, 7 parts of lecithin, 20 parts of Polyethylene glycol-2000s, 100 parts
Sterile water.
Comparative example 1: it is essentially identical with the preparation method of embodiment 1, it has only the difference is that lacking activated basic protease and adds
Add agent.
Comparative example 2: it is essentially identical with the preparation method of embodiment 1, it has only the difference is that lacking surfactant.
Comparative example 3: it is essentially identical with the preparation method of embodiment 1, it has only the difference is that lacking the source additive that reduces phlegm and internal heat.
Comparative example 4: it is essentially identical with the preparation method of embodiment 1, it has only the difference is that lacking auxiliary enzyme additive.
Comparative example 5: the multienzyme cleaning agent of Hangzhou company production.
By embodiment and comparative example multienzyme cleaning agent according to QB/T 1803-1993, GB 15979-2002 standard into
Row test, test result are as shown in table 1.
Table 1:
| Test item | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| Soil removal efficiency (%) | 99.5 | 98.4 | 98.9 | 97.1 | 97.6 | 96.5 | 97.2 | 83.4 |
| Protein residues rate (%) | 0.2 | 0.6 | 0.4 | 3.3 | 3.1 | 3.2 | 3.7 | 4 |
| Bloodstain residual rate (%) | 0.01 | 0.05 | 0.03 | 0.15 | 0.13 | 0.17 | 0.29 | 0.8 |
| Sterilizing rate (%) | 99.8 | 99.3 | 99.5 | 97.2 | 96.5 | 96.3 | 95.4 | 81.6 |
The foregoing is merely preferred modes of the invention, are not intended to limit the invention, it is all the spirit and principles in the present invention it
Interior, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.In summary, originally
The medical instrument multienzyme cleaning agent of invention is more preferable compared to commercial product effect, is worth promoting.
Claims (7)
1. a kind of medical instrument multienzyme cleaning agent, according to parts by weight, including following component, 4 ~ 7 parts of lecithin, 10 ~ 20 parts it is poly-
Ethylene glycol -2000,80 ~ 100 parts of sterile waters, which is characterized in that further include 10 ~ 20 parts of activated basic albumen enzyme additives, 5 ~ 9 parts
Surfactant, 2 ~ 6 parts of source additives that reduce phlegm and internal heat, 5 ~ 10 parts of auxiliary enzyme additives.
2. medical instrument multienzyme cleaning agent according to claim 1, which is characterized in that the activated basic protease addition
Agent the preparation method comprises the following steps:
(1) according to the mass fraction, 3 ~ 6 parts of calcium chloride, 0.5 ~ 0.8 part of 4- formylphenyl boronic acid, 1 ~ 3 part of propylene glycol monomethyl ether, 5 are taken
~ 10 parts of glycerine, 3 ~ 8 parts of alkali proteases are stirred, and obtain stirring mixture, take Pluronic-F88 1 ~ 3:20 in mass ratio
~ 30 are added distilled water mixing, obtain mixed liquor a, take beta-cyclodextrin 1 ~ 3:90 in mass ratio ~ 100 that distilled water mixing is added, must mix
Liquid b；
(2) it takes stirring mixture 2 ~ 5:80 in mass ratio ~ 100 that mixed liquor b is added, is stirred in 20 ~ 25 DEG C, then stirring is added dropwise
The mixed liquor a of mixture quality 70 ~ 80% controls 30 ~ 50min of time for adding, keeps the temperature in 25 ~ 30 DEG C, and centrifugation takes pellet frozen
Drying is to get activated basic albumen enzyme additive.
3. medical instrument multienzyme cleaning agent according to claim 2, which is characterized in that be stirred in the step (1)
Object is stirred condition are as follows: is stirred 30 ~ 50min in 25 ~ 30 DEG C.
4. medical instrument multienzyme cleaning agent according to claim 1, which is characterized in that the preparation side of the surfactant
Method are as follows:
S1. according to the mass fraction, take 3 ~ 8 parts of octamethylcy-clotetrasiloxanes, 2 ~ 6 parts of hexamethyldisiloxane, 10 ~ 20 parts of height hydrogeneous
Silicone oil is passed through nitrogen protection, is stirred, and must stir mixture a, takes stirring mixture a 40 ~ 50:1 in mass ratio ~ 3 that sulphur is added
Acid keeps the temperature in 65 ~ 75 DEG C, is cooled to room temperature, obtains cooled material, takes cooled material 10 ~ 20:3 in mass ratio ~ 5 that sodium bicarbonate is added and stirs
Mixing is mixed, is filtered, is taken filtrate decompression to distill, obtain vacuum distillation object；
S2. N is taken, vacuum distillation object is added in N- dimethylallylamine 3 ~ 5:8 in mass ratio ~ 10, it is passed through nitrogen protection, then plus
Entering to be evaporated under reduced pressure 1,3- divinyl -1,1 of amount of substance 3 ~ 5%, 3,3- tetramethyl disiloxane platinum are kept the temperature in 110 ~ 120 DEG C,
Vacuum distillation, obtains vacuum distillation object, takes vacuum distillation object 3 ~ 6:8 in mass ratio ~ 10 that isopropanol is added, is passed through nitrogen protection, drips
Plus-minus pressure distillation amount of substance 30 ~ 40% sodium chloroacetate be stirred, rotary evaporation, take rotary evaporation object 1 ~ 3:7 in mass ratio ~
10 are added acetone, keep the temperature in 2 ~ 5 DEG C, take precipitating dry to get surfactant.
5. medical instrument multienzyme cleaning agent according to claim 4, which is characterized in that stir mixture in the step S1
A's is stirred condition are as follows: is stirred 20 ~ 30min in 60 ~ 80 DEG C.
6. medical instrument multienzyme cleaning agent according to claim 1, which is characterized in that the source additive that reduces phlegm and internal heat are as follows: take
Scutelloside 2 ~ 5:1 in mass ratio ~ 3 be added Hemarisin mixing to get.
7. medical instrument multienzyme cleaning agent according to claim 1, which is characterized in that the auxiliary enzyme additive are as follows: take
Lysozyme, amylase 6 ~ 9:2 in mass ratio ~ 5:2 ~ 6 be added lipase mixing to get.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113930293A (en) * | 2021-10-14 | 2022-01-14 | 山东消博士消毒科技股份有限公司 | Rapid and efficient multienzyme cleaning agent and preparation method thereof |
| CN114106943A (en) * | 2021-11-08 | 2022-03-01 | 山东消博士消毒科技股份有限公司 | Multienzyme cleaning agent and processing equipment thereof |
| CN114214125A (en) * | 2021-12-24 | 2022-03-22 | 江苏视科新材料股份有限公司 | Optical resin lens mold cleaning agent |
| CN114276873A (en) * | 2021-12-24 | 2022-04-05 | 江苏视科新材料股份有限公司 | Preparation method of optical resin lens mold cleaning agent |
| CN116425979A (en) * | 2023-05-24 | 2023-07-14 | 西南石油大学 | Betaine polysiloxane, preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106281761A (en) * | 2016-08-19 | 2017-01-04 | 南京巨鲨显示科技有限公司 | A kind of medical apparatus and instruments multienzyme cleaning agent and preparation method thereof |
| CN106893647A (en) * | 2017-02-28 | 2017-06-27 | 长安大学 | A kind of multienzyme medicine equipment cleaning agent and preparation method thereof |
| US20170216471A1 (en) * | 2015-11-10 | 2017-08-03 | American Sterilizer Company | Cleaning and disinfecting composition |
-
2018
- 2018-09-08 CN CN201811046808.8A patent/CN108949381B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170216471A1 (en) * | 2015-11-10 | 2017-08-03 | American Sterilizer Company | Cleaning and disinfecting composition |
| CN108350396A (en) * | 2015-11-10 | 2018-07-31 | 美国消毒公司 | Cleaning and sanitizing composition |
| CN106281761A (en) * | 2016-08-19 | 2017-01-04 | 南京巨鲨显示科技有限公司 | A kind of medical apparatus and instruments multienzyme cleaning agent and preparation method thereof |
| CN106893647A (en) * | 2017-02-28 | 2017-06-27 | 长安大学 | A kind of multienzyme medicine equipment cleaning agent and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113930293A (en) * | 2021-10-14 | 2022-01-14 | 山东消博士消毒科技股份有限公司 | Rapid and efficient multienzyme cleaning agent and preparation method thereof |
| CN114106943A (en) * | 2021-11-08 | 2022-03-01 | 山东消博士消毒科技股份有限公司 | Multienzyme cleaning agent and processing equipment thereof |
| CN114214125A (en) * | 2021-12-24 | 2022-03-22 | 江苏视科新材料股份有限公司 | Optical resin lens mold cleaning agent |
| CN114276873A (en) * | 2021-12-24 | 2022-04-05 | 江苏视科新材料股份有限公司 | Preparation method of optical resin lens mold cleaning agent |
| CN114276873B (en) * | 2021-12-24 | 2023-08-29 | 江苏视科新材料股份有限公司 | Preparation method of optical resin lens mold cleaning agent |
| CN116425979A (en) * | 2023-05-24 | 2023-07-14 | 西南石油大学 | Betaine polysiloxane, preparation method and application thereof |
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