CN108948150A - A kind of anti-aphid synergy small peptide and its application - Google Patents
A kind of anti-aphid synergy small peptide and its application Download PDFInfo
- Publication number
- CN108948150A CN108948150A CN201810797415.4A CN201810797415A CN108948150A CN 108948150 A CN108948150 A CN 108948150A CN 201810797415 A CN201810797415 A CN 201810797415A CN 108948150 A CN108948150 A CN 108948150A
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- CN
- China
- Prior art keywords
- aphid
- small peptide
- synergy
- chitinase
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of small peptides of anti-aphid synergy and its application, the small peptide can be used for aphid prevention and control, as the anti-aphid synergy small peptide of chitinase, solves the problems, such as that chitinase is insufficient to aphid inhibitory effect.
Description
Technical field
The present invention relates to a kind of anti-aphid synergy small peptide and its applications.
Background technique
Aphid is the piercing sucking insect of a kind of figure very little, can pass through the modes such as feeding, honeydew pollution, transmitted virus
The plants such as grain, cotton and tobacco are endangered, cause plant strain growth is slow, wilts, and induces a variety of virosis, black points etc., sternly
It can lead to plant death when weight, be important agriculture and forestry pest.So anti-eliminate aphis with the double meaning for controlling worm diseases prevention.
Chitinase is the enzyme for being catalyzed chitin hydrolysis and generating N-acetyl-glucosamine reaction.Part chitinase has aphid
There is inhibiting effect, but inhibitory effect is not obvious, and cannot still be applied to the anti-aphid of production estimation.
Summary of the invention
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of anti-aphid synergy small peptide and its answer
With solving the problems in above-mentioned background technique.
The technical solution adopted by the present invention to solve the technical problems is: providing a kind of small peptide of anti-aphid synergy, forms
The amino acid sequence of small peptide is SEQ ID No:01, i.e. Asn Ser Ser Val Ser Ile Ile.
The present invention also provides application of the above-mentioned small peptide in aphid prevention and control.
In a preferred embodiment of the present invention, the small peptide kills aphid effect for synergy chitinase.
In a preferred embodiment of the present invention, the small peptide is used in conjunction with chitinase, the small peptide and chitin
The concentration ratio of enzyme is X:1, and wherein X is greater than 1.
The technical program compared with the background art, it has the following advantages:
The small peptide can be used for aphid prevention and control, as the anti-aphid synergy small peptide of chitinase, effectively increase chitinase to aphid
Worm inhibitory effect makes it have the practicability in production.
Specific embodiment
Embodiment 1
A kind of small peptide of anti-aphid synergy of the present embodiment, the small peptide utilize solid-state chemical reaction method, form the amino of small peptide
Acid sequence is SEQ ID No:01, i.e. Asn Ser Ser Val Ser Ile Ile.
Embodiment 2
Application of the small peptide of embodiment 1 in aphid prevention and control, be mainly used for synergy chitinase kills aphid effect.
Concrete operations are to be used in conjunction with the small peptide with chitinase, and the concentration ratio of the small peptide and chitinase is 2:
1。
Synergistic effect of the above-mentioned small peptide to chitinase, using chitinase Tma12, experimental procedure is as follows:
(1) 180 4 age black peach aphid is divided into 12 groups, every group 15；
(2) membrane vesicle method is raised:
1. a membrane vesicle to be covered on to one end of a tubular bracket, artificial medium/nutrient solution is filled in membrane vesicle；
2. several aphids are placed on the membrane vesicle in above-mentioned tubular bracket by writing brush；
3. the other end in above-mentioned tubular bracket covers one layer of light transmission ventilation film；
4. 18~20 DEG C indoors, raising 3 days under the conditions of humidity 50-75%, illumination 16L:8D, it is average that observation calculates black peach aphid
Survival rate.
1 embodiment of table, 2 experimental result
The nutrient solution is equivalent to the water of dissolution small peptide, chitinase, and the small peptide of freeze-drying or chitinase powder are weighed
Adequate nutrition liquid is added after a certain amount of, is configured to 100 μ g/mL small peptides and 50 μ g/mL chitinases for measuring and kills aphid activity, institute
The main configuration method for stating nutrient solution is as follows :)
Each nutriment is weighed according to table 2,3,4, while 30g sucrose need to be added in every 100mL nutrient solution, it is molten with deionized water
Solution mix, be settled to 100mL, in super-clean bench with 0.22 or 0.45 μm of filtering with microporous membrane, -20 DEG C of freezen protectives.
2 aphid nutrient solution prescription (amino acid) of table
3 aphid nutrient solution prescription (vitamin) of table
4 aphid nutrient solution prescription (inorganic salts) of table
The above results show that small peptide of the present invention can significantly increase chitinase to the inhibiting effect of black peach aphid.
The above is only the preferred embodiment of the present invention, the range implemented of the present invention that therefore, it cannot be limited according to, i.e., according to
Equivalent changes and modifications made by the invention patent range and description, should still be within the scope of the present invention.
Sequence table
<110>Huaqiao University
<120>a kind of anti-aphid synergy small peptide and its application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 7
<212> PRT
<213>artificial sequence (Artificial Sequence)
<400> 1
Asn Ser Ser Val Ser Ile Ile
1 5
Claims (4)
1. a kind of small peptide of anti-aphid synergy, it is characterised in that: the amino acid sequence for forming small peptide is SEQ ID No:01.
2. application of the small peptide described in claim 1 in aphid prevention and control.
3. application according to claim 2, it is characterised in that: the small peptide kills aphid effect for synergy chitinase.
4. application according to claim 2, it is characterised in that: the small peptide is used in conjunction with chitinase, it is described short
The concentration of peptide and chitinase ratio is X:1, and wherein X is greater than 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810797415.4A CN108948150B (en) | 2018-07-19 | 2018-07-19 | Anti-aphid synergistic short peptide and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810797415.4A CN108948150B (en) | 2018-07-19 | 2018-07-19 | Anti-aphid synergistic short peptide and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108948150A true CN108948150A (en) | 2018-12-07 |
| CN108948150B CN108948150B (en) | 2021-06-29 |
Family
ID=64481799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810797415.4A Active CN108948150B (en) | 2018-07-19 | 2018-07-19 | Anti-aphid synergistic short peptide and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108948150B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597938A (en) * | 2004-09-22 | 2005-03-23 | 山西大学 | Recombined rhabdovirus containing double valence insect resisting gene |
| CN106967629A (en) * | 2016-11-21 | 2017-07-21 | 云南大学 | One plant of soil actinomycete and its anti-application that eliminates aphis |
| CN107085094A (en) * | 2017-06-15 | 2017-08-22 | 华侨大学 | A kind of indoor bioassay method for killing aphid albumen for being directed to aphid |
-
2018
- 2018-07-19 CN CN201810797415.4A patent/CN108948150B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597938A (en) * | 2004-09-22 | 2005-03-23 | 山西大学 | Recombined rhabdovirus containing double valence insect resisting gene |
| CN106967629A (en) * | 2016-11-21 | 2017-07-21 | 云南大学 | One plant of soil actinomycete and its anti-application that eliminates aphis |
| CN107085094A (en) * | 2017-06-15 | 2017-08-22 | 华侨大学 | A kind of indoor bioassay method for killing aphid albumen for being directed to aphid |
Non-Patent Citations (2)
| Title |
|---|
| 温扬帆: "害虫体内Cry结合蛋白的鉴定及其与Cry毒素的互作研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 蔡亚君: "几丁质酶和杀虫晶体蛋白对蚊虫协同毒杀作用研究", 《中国优秀博士学位论文全文数据库 农业科技辑》 * |
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| Publication number | Publication date |
|---|---|
| CN108948150B (en) | 2021-06-29 |
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