CN103651594A - Compound biological pesticide - Google Patents
Compound biological pesticide Download PDFInfo
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- CN103651594A CN103651594A CN201310699916.6A CN201310699916A CN103651594A CN 103651594 A CN103651594 A CN 103651594A CN 201310699916 A CN201310699916 A CN 201310699916A CN 103651594 A CN103651594 A CN 103651594A
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Abstract
The invention discloses a compound biological pesticide and a preparation method thereof. The compound biological pesticide is prepared by components in parts by weight as follows: 0.1 part of lentinan, 1-5 parts of amino acid, 10-20 parts of bupleurum, 10-30 parts of codonopsis pilosula, 10-20 parts of aconitine, 15-20 parts of radix euphorbiae lantu, 15-25 parts of ephedra and 1-5 parts of a suspending aid. Compared with ordinary chemical pesticide in the prior art, the prepared compound biological pesticide has the characteristics of good effect and low toxicity and can be used as an ideal environment-friendly agricultural plant protection drug; meanwhile, two or more pesticides are compounded, so that on one hand, the bactericidal and insecticidal spectrum can be widened, and on the other hand, the pesticide effect can be improved; and more importantly, the problem of drug resistance due to the fact that a single pesticide is used for a long term is solved.
Description
Technical field
The present invention relates to a kind of biopesticide, be specifically related to a kind of mainly by the made composite biological insecticide of plurality of Chinese, be applied to agriculture field.
Background technology
Biological living and metabolite and the transgene product of the pests such as biopesticide refers to for anti-curing the disease, worm, grass, and can be made into the biogenic preparation of commodity, comprise the genetically modified plants of bacterium, virus, fungi, nematode, plant, Natural Enemies of Insects, farm antibiotics, plant growth regulator and Resistant crop smothering etc.Biopesticide is applied to the existing semicentennial history of agricultural production, but development is always comparatively slow for various reasons.In recent years, people more and more note the sustainable development of agricultural production and the coordination of human and environment, particularly difficult due to the toxic and side effect of chemical pesticide and screening novel pesticide, makes enterprise and researcher start notice to turn to research and development and the use of biopesticide.
Normally used chemical pesticide toxicity is large, in crops, have certain residual, thereby the mankind are had to potential threat.And often use the insecticide of single formula, not satisfactory for the effect of desinsection.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of composite biological insecticide.
Technical scheme: for achieving the above object, a kind of composite biological insecticide provided by the invention, it is mainly made by the component of following weight ratio: 0.1 part of lentinan, amino acid/11-5 part, radix bupleuri 10-20 part, Radix Codonopsis 10-30 part, aconitine 10-20 part, root of langdu 15-20 part, Chinese ephedra 15-25 part, suspending agent 1-5 part.
Preferably, above-mentioned composite biological insecticide is mainly made by the component of following weight ratio: 0.1 part of lentinan, amino acid 2-4 part, radix bupleuri 14-18 part, Radix Codonopsis 15-25 part, aconitine 12-18 part, root of langdu 16-18 part, Chinese ephedra 18-22 part, suspending agent 2-4 part.
Preferably, described suspending agent is a kind of in sodium alginate, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, gum Arabic.
The present invention also provides the preparation method of above-mentioned composite biological insecticide, and it is made by following steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 6-8 times of volume, decoct 2-3 time, each 2-4 hour, merges extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.05-1.10;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add suspending agent, obtain.
Beneficial effect: the present invention is with respect to prior art, and the composite biological insecticide of gained, compares with general chemical pesticide, has the feature effective, toxicity is low, can be used as desirable environmentally friendly agricultural plant protection medicine.Meanwhile, the composite one side of two or more agricultural chemicals can expand sterilization and disinsection spectrum, can also promote drug effect on the other hand, the more important thing is and can solve the drug-fast problem that the single agricultural chemicals of long-term use causes.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
Prescription: 0.1 part of lentinan, amino acid/11 part, 10 parts of radix bupleuri, 10 parts of Radix Codonopsis, 10 parts of aconitines, 15 parts of the roots of langdu, 15 parts, Chinese ephedra, 1 part of sodium alginate.
The preparation method of above-mentioned composite biological insecticide, it comprises the steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 6 times of volumes, decoct 2 times, each 2 hours, merge extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.05;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add sodium alginate, obtain.
Embodiment 2:
Prescription: 0.1 part of lentinan, 5 parts, amino acid, 20 parts of radix bupleuri, 30 parts of Radix Codonopsis, 20 parts of aconitines, 20 parts of the roots of langdu, 25 parts, Chinese ephedra, 5 parts of methylcellulose.
The preparation method of above-mentioned composite biological insecticide, it comprises the steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 8 times of volumes, decoct 3 times, each 4 hours, merge extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.10;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add methylcellulose, obtain.
Embodiment 3:
Prescription: 0.1 part of lentinan, 3 parts, amino acid, 15 parts of radix bupleuri, 20 parts of Radix Codonopsis, 15 parts of aconitines, 18 parts of the roots of langdu, 20 parts, Chinese ephedra, 3 parts of sodium carboxymethylcelluloses.
The preparation method of above-mentioned composite biological insecticide, it comprises the steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 7 times of volumes, decoct 2 times, each 3 hours, merge extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.08;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add sodium carboxymethylcellulose, obtain.
Embodiment 4:
Prescription: 0.1 part of lentinan, 2 parts, amino acid, 14 parts of radix bupleuri, 15 parts of Radix Codonopsis, 12 parts of aconitines, 16 parts of the roots of langdu, 18 parts, Chinese ephedra, 2 parts of hydroxypropyl celluloses.
The preparation method of above-mentioned composite biological insecticide, it comprises the steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 6 times of volumes, decoct 2 times, each 4 hours, merge extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.06;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add hydroxypropyl cellulose, obtain.
Embodiment 5:
Prescription: 0.1 part of lentinan, 4 parts, amino acid, 18 parts of radix bupleuri, 25 parts of Radix Codonopsis, 18 parts of aconitines, 18 parts of the roots of langdu, 22 parts, Chinese ephedra, 4 parts of gum Arabics.
The preparation method of above-mentioned composite biological insecticide, it comprises the steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 7 times of volumes, decoct 3 times, each 3 hours, merge extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.08;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add gum Arabic, obtain.
Experimental example gained insecticide of the present invention the results are shown in Table 1 to the effect experiment of diamondback moth larvae
Test is the diamond-back moth of raising in indoor continuous generation for examination worm source, and test method adopts blade infusion process;
Common lentinan solution, common Chinese extract solution, lentinan and the simple mixed solution of Chinese medical extract all refer to and add according to a conventional method the made solution of pure water, be made as respectively control group A, control group B and control group C.
Above-mentioned Chinese medical extract refers to that Chinese medicine described in the present invention is according to the gained of extracting method described in the present invention.
Drug dose is desinsection routine dose;
Lethality after medicine: refer to after drug administration that, after certain hour, death toll accounts for the percentage of total test number.
The effect experiment result of table 1 gained insecticide of the present invention to diamondback moth larvae
Note: with A control group, B control group and the comparison of C control group, * P<0.05
As seen from the above table, control group C compares with control group A, control group B, and after medicine, behind 1 day, medicine, after 3 days and medicine, 7 days lethality all do not have marked change.And the composite biological insecticide of gained of the present invention is compared with control group A, B and C, after medicine, behind 1 day, medicine, after 3 days and medicine, 7 days lethality all obviously improve, and illustrate that insecticide insecticidal effect of the present invention significantly improves.
Claims (4)
1. a composite biological insecticide, it is characterized in that, it is mainly made by the component of following weight ratio: 0.1 part of lentinan, amino acid/11-5 part, radix bupleuri 10-20 part, Radix Codonopsis 10-30 part, aconitine 10-20 part, root of langdu 15-20 part, Chinese ephedra 15-25 part, suspending agent 1-5 part.
2. a kind of composite biological insecticide according to claim 1, is characterized in that: it is mainly made by the component of following weight ratio: 0.1 part of lentinan, amino acid 2-4 part, radix bupleuri 14-18 part, Radix Codonopsis 15-25 part, aconitine 12-18 part, root of langdu 16-18 part, Chinese ephedra 18-22 part, suspending agent 2-4 part.
3. a kind of composite biological insecticide according to claim 1, is characterized in that: described suspending agent is a kind of in sodium alginate, methylcellulose, sodium carboxymethylcellulose, hydroxypropyl cellulose, gum Arabic.
4. the preparation method of a kind of composite biological insecticide claimed in claim 1, is characterized in that: it is made by following steps:
(1) radix bupleuri, Radix Codonopsis, aconitine, the root of langdu, Chinese ephedra are cleaned, added the pure water of 6-8 times of volume, decoct 2-3 time, each 2-4 hour, merges extract, the liquid extract that while being concentrated into 30 ℃, relative density is 1.05-1.10;
(2) lentinan and amino acid are dissolved with pure water, obtain lentinan solution;
(3) above-mentioned liquid extract and lentinan solution are mixed, 4 ℃ of refrigerations are placed and are spent the night, and obtain mixed liquor;
(4) above-mentioned mixed liquor is filtered, add suspending agent, obtain.
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| CN201310699916.6A CN103651594B (en) | 2013-12-18 | 2013-12-18 | A kind of composite biological insecticide |
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| CN201310699916.6A CN103651594B (en) | 2013-12-18 | 2013-12-18 | A kind of composite biological insecticide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104522060A (en) * | 2014-12-31 | 2015-04-22 | 江苏健神生物农化有限公司 | Environment-friendly plant pesticide and preparation method thereof |
| CN104886156A (en) * | 2015-05-13 | 2015-09-09 | 卞佳林 | Cotton bollworm pesticide |
| CN105248488A (en) * | 2015-09-30 | 2016-01-20 | 界首市保华种植专业合作社 | Biological insecticide containing brucea javanica |
| CN106719936A (en) * | 2016-11-30 | 2017-05-31 | 河池市金城江区科学技术情报研究所 | It is exclusively used in insecticide of mulberry tree plantation and preparation method thereof |
| KR20190057010A (en) * | 2017-11-17 | 2019-05-27 | 한국생명공학연구원 | Pest control composition comprising Codonopsis pilosula (Franch.) Nannf. extract as active ingredient |
| CN110074142A (en) * | 2019-04-24 | 2019-08-02 | 河北省科学院生物研究所 | Application and insecticide and preparation method thereof of the lentinus edodes strain stick in pest control |
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| CN1100594A (en) * | 1993-09-23 | 1995-03-29 | 李百川 | Pesticide for fruit trees and method for prepn. of same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104522060A (en) * | 2014-12-31 | 2015-04-22 | 江苏健神生物农化有限公司 | Environment-friendly plant pesticide and preparation method thereof |
| CN104886156A (en) * | 2015-05-13 | 2015-09-09 | 卞佳林 | Cotton bollworm pesticide |
| CN105248488A (en) * | 2015-09-30 | 2016-01-20 | 界首市保华种植专业合作社 | Biological insecticide containing brucea javanica |
| CN106719936A (en) * | 2016-11-30 | 2017-05-31 | 河池市金城江区科学技术情报研究所 | It is exclusively used in insecticide of mulberry tree plantation and preparation method thereof |
| KR20190057010A (en) * | 2017-11-17 | 2019-05-27 | 한국생명공학연구원 | Pest control composition comprising Codonopsis pilosula (Franch.) Nannf. extract as active ingredient |
| KR102255904B1 (en) * | 2017-11-17 | 2021-05-26 | 한국생명공학연구원 | Pest control composition comprising Codonopsis pilosula (Franch.) Nannf. extract as active ingredient |
| CN110074142A (en) * | 2019-04-24 | 2019-08-02 | 河北省科学院生物研究所 | Application and insecticide and preparation method thereof of the lentinus edodes strain stick in pest control |
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| CN103651594B (en) | 2015-08-05 |
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Effective date of registration: 20201216 Address after: No.1, community 10, shizitian village, Lijiang Town, Hejiang County, Luzhou City, Sichuan Province, 646000 Patentee after: Luzhou Yiyun Biotechnology Co.,Ltd. Address before: 266005 room 219, Shenxian Road, Shinan District, Qingdao, Shandong, 219 Patentee before: QINGDAO BAIRUIJI BIOTECHNOLOGY Co.,Ltd. |
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