CN108935345A - A kind of device for testing small-sized moth class insect olfaction behavior - Google Patents
A kind of device for testing small-sized moth class insect olfaction behavior Download PDFInfo
- Publication number
- CN108935345A CN108935345A CN201811049840.1A CN201811049840A CN108935345A CN 108935345 A CN108935345 A CN 108935345A CN 201811049840 A CN201811049840 A CN 201811049840A CN 108935345 A CN108935345 A CN 108935345A
- Authority
- CN
- China
- Prior art keywords
- gas
- docked
- guide tube
- filter column
- test worm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
Abstract
The invention discloses a kind of devices for testing small-sized moth class insect olfaction behavior, it includes to be located at mini air pump (1), it is docked by gas-guide tube with active carbon Filter column (3) outlet side of the mini air pump (1), it is docked by gas-guide tube with gas flowmeter (4) outlet side of the active carbon Filter column (3), it is docked by gas-guide tube with water Filter column (6) outlet side of the gas flowmeter (4), it is docked by gas-guide tube with threeway (18) outlet side of the water Filter column (6), it is docked by gas-guide tube performance testing device at the other both ends of the threeway (18).The present invention can once test 20-30 test worms to the behavior reaction of odoring substance to be measured simultaneously, and working efficiency significantly improves；The care intensity that cold light source incandescent lamp experiences the indoor test worm of activity in all directions always, can effectively avoid the movement orientation of the differentia influence test worm because of bright and dark light, make action selection to different odor stimulation to influence test worm.
Description
Technical field
The present invention relates to the relevant technical fields of Bioexperiment equipment, and in particular to be a kind of small-sized moth class of test
The device of insect olfaction behavior.
Background technique
Insect obtains sensitive nasal receptor organ in long-term evolutionary process, can be by olfactory system insect
Certain volatile infochemicals are specifically identified in complex environment.Many behaviors of insect such as seek a spouse mating, post
Master positioning, ovipotion site selection, the predation of escape natural enemy, aggregation, migration etc. are by the induction of odoring substance and regulation.People's root
According to this theory, the behavior for how regulating and controlling insect to odoring substance is had conducted extensive research, and lures or walk quickly and keep away work it is expected that will have
Odoring substance is applied in the Unpolluting management of pest.
Lepidoptera pest is to carry out Unpolluting management insect the most successful currently with gyplure and botanical attractant
Monoid.The gyplure of lepidopterous insects is only capable of luring male worm, does not have attractive activity to female adult, in addition most of lepidopterous insects tools
There is the habit of multiple mating, in the farmland or orchard of the male worm population quantity polar region of gyplure prevention and treatment, female adult can also be obtained
Higher mating rate causes the control efficiency of gyplure to substantially reduce.In order to trap female adult, researcher is desirable in evil
The odoring substance that can lure female adult is screened in the most thermophilic host plant of worm, passes through host plant volatile and sex pheromone
After synergy attractive activity can be all had to male worm and female adult.Currently, screening there is the host of attractive activity to plant female adult
Object volatile matter is the hot and difficult issue problem of research.In the biological test of the small-sized the moth pests Olfactory behavior of Lepidoptera, at present
It is mainly carried out by Y-shaped olfactometer, Y-shaped olfactometer is only capable of 1 test worm of test, since working efficiency is lower, test every time
It measures larger, it is desirable that test worm makes a choice within 10min, and the interference of the external experimental facilities noise such as air pump, leads to test worm in addition
Selection rate it is lower.In addition, the contingency that test worm is orientated selection to the movement of control tube and smell pipe is larger, result reliability compared with
Difference.
Summary of the invention
A kind of survey provided the invention aims to preferably study the Olfactory behavior of the small-sized moth class insect of Lepidoptera
The device of small-sized moth class insect olfaction behavior is tried, the problem of it can be efficiently solved in the presence of background technique.
In the presence of solving the problems, such as background technique, it includes to be located at mini air pump 1, the mini air pump 1
Outlet side is docked by gas-guide tube with active carbon Filter column 3, and the outlet side of the active carbon Filter column 3 passes through gas-guide tube and gas
Flowmeter 4 docks, and the outlet side of the gas flowmeter 4 is docked by gas-guide tube with water Filter column 6, and the water Filter column 6 goes out
Gas end is docked by gas-guide tube with threeway 18, and the other both ends of the threeway 18 are docked by gas-guide tube performance testing device, described
Performance testing device include the first odor source trapping chamber 8 and the second odor source trapping chamber 20 and test worm recreation room 9, described the
One odor source trapping chamber 8 and the second odor source trapping chamber 20 are symmetricly set on the end of test worm recreation room 9, first odor source
Trapping chamber 8 is docked by the first tubaeform connecting tube 10 with test worm recreation room 9, and the second odor source trapping chamber 20 passes through second
Tubaeform connecting tube 19 is docked with test worm recreation room 9, the test worm recreation room 9 and the first odor source trapping chamber 8 and the second smell
The end of 20 opposite side of source trapping chamber is provided with test worm liberation port 12；
Mini air pump 1, active carbon Filter column 3, gas flowmeter 4, water Filter column 6 and the threeway 18 and performance testing
Device is arranged in light control room bracket 14, and the light control room bracket 14 is generally mutually spliced by bar shaped profile
The cuboid framework structure constituted, 14 upper cover of light control room bracket are buckled with a floor gobo 17.
The bottom of the gas flowmeter 4 is provided with the gas flowmeter bracket 5 that cooperation is socketed with it.
The bottom setting recreation room bracket 13 of the test worm recreation room 9, the generally rectangular parallelepiped structure of recreation room bracket 13,
Its middle part offers a groove for the insertion of 9 gap of test worm recreation room.
Cold light source white heat 15 and timing control switch 16 are installed on the inner wall of light control room bracket 14.
Due to using above technical scheme, the invention has the following advantages:
1) 20-30 test worms can be once tested simultaneously to the behavior reaction of odoring substance to be measured, and working efficiency significantly improves.
2) the care intensity that cold light source incandescent lamp experiences the indoor test worm of activity in all directions always, can effectively be kept away
Exempt from the movement orientation of the differentia influence test worm because of bright and dark light, action selection is made to different odor stimulation to influence test worm.
3) active carbon Filter column, which can effectively remove, mixes aerial background odoring substance, obtains relatively pure air
Stream.
4) on the one hand the first tubaeform connecting tube and the second tubaeform connecting tube increase test worm in recreation room and experience smell
The area of material incentive makes test worm be easier to discriminative stimulus source to induce it to make action selection；Secondly, being trapped into odor source
The test worm of room limits its return recreation room using the small-bore of tubaeform connection tube end, this design can accurately reflect test worm to gas
The behavior reaction of taste substance, experimental result are relatively reliable.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention without any creative labor, may be used also for those of ordinary skill in the art
To obtain other drawings based on these drawings.
Fig. 1 is decomposition texture schematic diagram of the invention；
Fig. 2 is the assembly structure diagram of invention.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below will
In conjunction with the attached drawing in the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described.
Embodiment 1
Referring to Fig. 1-2, present embodiment, which adopts the following technical solutions, to be achieved, it includes to be located at mini air pump 1,
The outlet side of the mini air pump 1 is docked by gas-guide tube with active carbon Filter column 3, the outlet side of the active carbon Filter column 3
It is docked by gas-guide tube with gas flowmeter 4, the outlet side of the gas flowmeter 4 is docked by gas-guide tube with water Filter column 6, institute
The outlet side for stating water Filter column 6 is docked by gas-guide tube with threeway 18, and the other both ends of the threeway 18 are surveyed by gas-guide tube behavior
Docking is set in trial assembly, and the performance testing device includes the first odor source trapping chamber 8 and the second odor source trapping chamber 20 and examination
Worm recreation room 9, the first odor source trapping chamber 8 and the second odor source trapping chamber 20 are symmetricly set on the end of test worm recreation room 9
Portion, the first odor source trapping chamber 8 are docked by the first tubaeform connecting tube 10 with test worm recreation room 9, second smell
Source trapping chamber 20 is docked by the second tubaeform connecting tube 19 with test worm recreation room 9, the test worm recreation room 9 and the first odor source
The end of 20 opposite side of trapping chamber 8 and the second odor source trapping chamber is provided with test worm liberation port 12；
Mini air pump 1, active carbon Filter column 3, gas flowmeter 4, water Filter column 6 and the threeway 18 and performance testing
Device is arranged in light control room bracket 14, and the light control room bracket 14 is generally mutually spliced by bar shaped profile
The cuboid framework structure constituted, 14 upper cover of light control room bracket are buckled with a floor gobo 17.
The bottom of the gas flowmeter 4 is provided with the gas flowmeter bracket 5 that cooperation is socketed with it.
The bottom setting recreation room bracket 13 of the test worm recreation room 9, the generally rectangular parallelepiped structure of recreation room bracket 13,
Its middle part offers a groove for the insertion of 9 gap of test worm recreation room.
Cold light source white heat 15 and timing control switch 16 are installed on the inner wall of light control room bracket 14.
Embodiment 2
Before experiment, make after the active carbon filled in active carbon Filter column 3 is first handled 2 h De contaminations in 220 DEG C of baking oven
With；When experiment, outside air is sent into the active carbon Filter column 3 being made of active carbon by the compression of mini air pump 1 filters first,
Filtered clean gas flow carries out further gas by entering water Filter column 6 after the adjusting air flow rate of gas flowmeter 4
Washing impurity-removing, the air-flow after second of cleaning enters the first odor source trapping chamber 8 of performance testing device and the second odor source lures
Collect room 20, and carries respective sample smell in the first odor source trapping chamber 8 and the second odor source trapping chamber 20 and pass through first respectively
Tubaeform connecting tube 10 and the second tubaeform connecting tube 19 enter in test worm recreation room 9, and test worm liberation port 12 is then used single layer
Hospital gauze sealing can be discharged with the mixed gas ensured in test worm recreation room 9.
Test worm introduces at recreation room liberation port 12, and test sample is placed on the first odor source trapping chamber 8, and control sample is put
It sets in the second odor source trapping chamber 20, the odoring substance of test sample volatilization passes through the first loudspeaker from the first odor source trapping chamber 8
Shape connecting tube 10 enters test worm recreation room 9；The odoring substance of reference substance volatilization passes through the second loudspeaker from the second odor source trapping chamber 20
Shape connecting tube 19 enters test worm recreation room 9；
In order to prevent because light unevenly has an impact the action selection of test worm, performance testing device is placed in light control room
In bracket 14, control room top surface, two sides and front respectively arrange that the cold light source incandescent lamp tube of 38 W, control outdoor use black hide
The processing of light cloth.Test worm generates behavior reaction after experiencing odoring substance: if odoring substance has attracting action, test worm meeting to test worm
Enter the first odor source trapping chamber 8 against the tubaeform connecting tube 10 in direction first of olfactory flow；If odoring substance is to examination
Worm has avoidance effect, and test worm can escape Odor stimulation, and the phenomenon that collecting around to test worm liberation port 12 is presented；If odoring substance
Test worm is not acted on, test worm will not show the phenomenon that significantly selecting or escaping.This device both can be control with blank
Single smell sample is tested to the behavior reaction of test worm, it can also behavior reaction of the more different samples to test worm two-by-two.Experiment
Be advisable with 6-8 h, after test worm number in recording odor source trapping chamber 8 and for statistical analysis.
It needs thoroughly to clean recreation room 9, the first odor source trapping chamber 8 and the second odor source with dehydrated alcohol before experiment every time and lure
Collect room 20 and all connecting tubes, then wash with distilled water for several times after dried in 50-60 DEG C of baking oven, after being sealed with aluminium-foil paper
It is spare.In order to guarantee the adsorption capacity of active carbon, experiment needs the active carbon after filled high-temperature De contamination every time.
The present invention is designed primarily directed to small-sized Lepidoptera moth class insect, by change gas flowmeter 4 flow velocity and
The bore of first tubaeform connecting tube 10 and the second tubaeform connecting tube 19, is suitable for the small-sized moth class insect of numerous Lepidopteras
Odorant-binding protein.But not after tested to the behavior reaction of the insects such as other mesh such as coleoptera, Orthoptera, Semiptera, behavior
Select effect unclear
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although reference
Invention is explained in detail for previous embodiment, those skilled in the art should understand that, it still can be right
Technical solution documented by foregoing embodiments is modified or equivalent replacement of some of the technical features；And this
It modifies or replaces, the spirit and model of technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (3)
1. a kind of device for testing small-sized moth class insect olfaction behavior, it is characterised in that it includes to be located at mini air pump (1), should
The outlet side of mini air pump (1) is docked by gas-guide tube with active carbon Filter column (3), and the active carbon Filter column (3) goes out
Gas end is docked by gas-guide tube with gas flowmeter (4), and the outlet side of the gas flowmeter (4) passes through gas-guide tube and water Filter column
(6) it docks, the outlet side of the water Filter column (6) is docked by gas-guide tube with threeway (18), the other both ends of the threeway (18)
It is docked by gas-guide tube performance testing device, the performance testing device includes the first odor source trapping chamber (8) and the second gas
Taste source trapping chamber (20) and test worm recreation room (9), the first odor source trapping chamber (8) and the second odor source trapping chamber (20)
It is symmetricly set on the end of test worm recreation room (9), the first odor source trapping chamber (8) passes through the first tubaeform connecting tube (10)
It is docked with test worm recreation room (9), the second odor source trapping chamber (20) is living by the second tubaeform connecting tube (19) and test worm
Dynamic room (9) docking, the test worm recreation room (9) and the first odor source trapping chamber (8) and the second odor source trapping chamber (20) are opposite
The end of one end is provided with test worm liberation port (12)；
Mini air pump (1), active carbon Filter column (3), gas flowmeter (4), water Filter column (6) and the threeway (18) with
And performance testing device is arranged in light control room bracket (14), the light control room bracket (14) is generally by item
Shape profile mutually splices constituted a cuboid framework structure, and the light control room bracket (14) upper cover is buckled with floor screening
Light black cloth (17).
2. a kind of device for testing small-sized moth class insect olfaction behavior according to claim 1, it is characterised in that the gas
The bottom of flowmeter body (4) is provided with the gas flowmeter bracket (5) that cooperation is socketed with it.
3. a kind of device for testing small-sized moth class insect olfaction behavior according to claim 1, it is characterised in that the examination
Recreation room bracket (13) are arranged in the bottom of worm recreation room (9), recreation room bracket (13) the generally rectangular parallelepiped structure, and middle part is opened
Groove equipped with one for the insertion of test worm recreation room (9) gap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811049840.1A CN108935345A (en) | 2018-09-10 | 2018-09-10 | A kind of device for testing small-sized moth class insect olfaction behavior |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811049840.1A CN108935345A (en) | 2018-09-10 | 2018-09-10 | A kind of device for testing small-sized moth class insect olfaction behavior |
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| Publication Number | Publication Date |
|---|---|
| CN108935345A true CN108935345A (en) | 2018-12-07 |
Family
ID=64476361
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811049840.1A Pending CN108935345A (en) | 2018-09-10 | 2018-09-10 | A kind of device for testing small-sized moth class insect olfaction behavior |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111937813A (en) * | 2020-07-13 | 2020-11-17 | 河南工业大学 | Insect behavior testing arrangement that keeps away |
| CN113973781A (en) * | 2021-08-31 | 2022-01-28 | 河南工业大学 | Device for selectively observing hosts by grain storage insects |
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| CN101766132A (en) * | 2008-12-29 | 2010-07-07 | 中国水产科学研究院东海水产研究所 | Test device for memory behavior of aquatic animal |
| CN202232595U (en) * | 2011-06-23 | 2012-05-30 | 中国人民解放军海军医学研究所 | Testing device for olfactory behaviors of insects |
| KR20120005927U (en) * | 2011-02-15 | 2012-08-23 | 한국광기술원 | Action analysis apparatus for the insect species habit |
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| CN203675831U (en) * | 2014-01-08 | 2014-07-02 | 沈阳医学院 | Training testing device for mice learning memory ethology |
| CN105815275A (en) * | 2015-01-06 | 2016-08-03 | 苏州卓凯生物技术有限公司 | System carrying out high throughput analysis for insect athletic ability |
| CN107691383A (en) * | 2017-11-12 | 2018-02-16 | 云南林业职业技术学院 | A kind of device for being used to distinguish insect smell hobby |
| CN207720993U (en) * | 2017-11-12 | 2018-08-14 | 云南林业职业技术学院 | A kind of device for distinguishing insect smell hobby |
| CN208925005U (en) * | 2018-09-10 | 2019-06-04 | 延安大学 | A kind of device of the small-sized moth class insect olfaction behavior of novel test |
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2018
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Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2008117094A2 (en) * | 2007-03-24 | 2008-10-02 | William Anthony Jonfia-Essien | A modified petterson olfactometer |
| CN101766132A (en) * | 2008-12-29 | 2010-07-07 | 中国水产科学研究院东海水产研究所 | Test device for memory behavior of aquatic animal |
| KR20120005927U (en) * | 2011-02-15 | 2012-08-23 | 한국광기술원 | Action analysis apparatus for the insect species habit |
| CN202232595U (en) * | 2011-06-23 | 2012-05-30 | 中国人民解放军海军医学研究所 | Testing device for olfactory behaviors of insects |
| CN203618079U (en) * | 2013-11-18 | 2014-05-28 | 华南农业大学 | Insect behavior observing and recording device |
| CN203675831U (en) * | 2014-01-08 | 2014-07-02 | 沈阳医学院 | Training testing device for mice learning memory ethology |
| CN105815275A (en) * | 2015-01-06 | 2016-08-03 | 苏州卓凯生物技术有限公司 | System carrying out high throughput analysis for insect athletic ability |
| CN107691383A (en) * | 2017-11-12 | 2018-02-16 | 云南林业职业技术学院 | A kind of device for being used to distinguish insect smell hobby |
| CN207720993U (en) * | 2017-11-12 | 2018-08-14 | 云南林业职业技术学院 | A kind of device for distinguishing insect smell hobby |
| CN208925005U (en) * | 2018-09-10 | 2019-06-04 | 延安大学 | A kind of device of the small-sized moth class insect olfaction behavior of novel test |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111937813A (en) * | 2020-07-13 | 2020-11-17 | 河南工业大学 | Insect behavior testing arrangement that keeps away |
| CN113973781A (en) * | 2021-08-31 | 2022-01-28 | 河南工业大学 | Device for selectively observing hosts by grain storage insects |
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