CN208925005U - A kind of device of the small-sized moth class insect olfaction behavior of novel test - Google Patents
A kind of device of the small-sized moth class insect olfaction behavior of novel test Download PDFInfo
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- CN208925005U CN208925005U CN201821473705.5U CN201821473705U CN208925005U CN 208925005 U CN208925005 U CN 208925005U CN 201821473705 U CN201821473705 U CN 201821473705U CN 208925005 U CN208925005 U CN 208925005U
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Abstract
The utility model discloses a kind of devices of the small-sized moth class insect olfaction behavior of novel test, it includes to be located at mini air pump (1), it is docked by gas-guide tube with active carbon Filter column (3) outlet side of the mini air pump (1), it is docked by gas-guide tube with gas flowmeter (4) outlet side of the active carbon Filter column (3), it is docked by gas-guide tube with water Filter column (6) outlet side of the gas flowmeter (4), it is docked by gas-guide tube with threeway (18) outlet side of the water Filter column (6), it is docked by gas-guide tube performance testing device at the other both ends of the threeway (18).The utility model can once test 20-30 test worms to the behavior reaction of odoring substance to be measured simultaneously, and working efficiency significantly improves；The care intensity that cold light source incandescent lamp experiences the indoor test worm of activity in all directions always, can effectively avoid the movement orientation of the differentia influence test worm because of bright and dark light, make action selection to different odor stimulation to influence test worm.
Description
Technical field
The utility model relates to the relevant technical fields of Bioexperiment equipment, and in particular to be a kind of novel test
The device of small-sized moth class insect olfaction behavior.
Background technique
Insect obtains sensitive nasal receptor organ in long-term evolutionary process, can be by olfactory system insect
Certain volatile infochemicals are specifically identified in complex environment.Many behaviors of insect such as seek a spouse mating, post
Master positioning, ovipotion site selection, the predation of escape natural enemy, aggregation, migration etc. are by the induction of odoring substance and regulation.People's root
According to this theory, the behavior for how regulating and controlling insect to odoring substance is had conducted extensive research, and lures or walk quickly and keep away work it is expected that will have
Odoring substance is applied in the Unpolluting management of pest.
Lepidoptera pest is to carry out Unpolluting management insect the most successful currently with gyplure and botanical attractant
Monoid.The gyplure of lepidopterous insects is only capable of luring male worm, does not have attractive activity to female adult, in addition most of lepidopterous insects tools
There is the habit of multiple mating, in the farmland or orchard of the male worm population quantity polar region of gyplure prevention and treatment, female adult can also be obtained
Higher mating rate causes the control efficiency of gyplure to substantially reduce.In order to trap female adult, researcher is desirable in evil
The odoring substance that can lure female adult is screened in the most thermophilic host plant of worm, passes through host plant volatile and sex pheromone
After synergy attractive activity can be all had to male worm and female adult.Currently, screening there is the host of attractive activity to plant female adult
Object volatile matter is the hot and difficult issue problem of research.In the biological test of the small-sized the moth pests Olfactory behavior of Lepidoptera, at present
It is mainly carried out by Y-shaped olfactometer, Y-shaped olfactometer is only capable of 1 test worm of test, since working efficiency is lower, test every time
It measures larger, it is desirable that test worm makes a choice within 10min, and the interference of the external experimental facilities noise such as air pump, leads to test worm in addition
Selection rate it is lower.In addition, the contingency that test worm is orientated selection to the movement of control tube and smell pipe is larger, result reliability compared with
Difference.
Summary of the invention
The utility model aim is one provided to preferably study the Olfactory behavior of the small-sized moth class insect of Lepidoptera
The problem of device of kind of the small-sized moth class insect olfaction behavior of novel test, it can be efficiently solved in the presence of background technique.
In the presence of solving the problems, such as background technique, it includes to be located at mini air pump 1, the mini air pump 1
Outlet side is docked by gas-guide tube with active carbon Filter column 3, and the outlet side of the active carbon Filter column 3 passes through gas-guide tube and gas
Flowmeter 4 docks, and the outlet side of the gas flowmeter 4 is docked by gas-guide tube with water Filter column 6, and the water Filter column 6 goes out
Gas end is docked by gas-guide tube with threeway 18, and the other both ends of the threeway 18 are docked by gas-guide tube performance testing device, described
Performance testing device include the first odor source trapping chamber 8 and the second odor source trapping chamber 20 and test worm recreation room 9, described the
One odor source trapping chamber 8 and the second odor source trapping chamber 20 are symmetricly set on the end of test worm recreation room 9, first odor source
Trapping chamber 8 is docked by the first tubaeform connecting tube 10 with test worm recreation room 9, and the second odor source trapping chamber 20 passes through second
Tubaeform connecting tube 19 is docked with test worm recreation room 9, the test worm recreation room 9 and the first odor source trapping chamber 8 and the second smell
The end of 20 opposite side of source trapping chamber is provided with test worm liberation port 12；
Mini air pump 1, active carbon Filter column 3, gas flowmeter 4, water Filter column 6 and the threeway 18 and behavior
Test device is arranged in light control room bracket 14, and the light control room bracket 14 is generally mutual by bar shaped profile
Splice constituted a cuboid framework structure, bracket 14 upper cover in the light control room is buckled with a floor gobo 17.
The bottom of the gas flowmeter 4 is provided with the gas flowmeter bracket 5 that cooperation is socketed with it.
The bottom setting recreation room bracket 13 of the test worm recreation room 9, the generally rectangular parallelepiped structure of recreation room bracket 13,
Its middle part offers a groove for the insertion of 9 gap of test worm recreation room.
Cold light source white heat 15 and timing control switch 16 are installed on the inner wall of light control room bracket 14.
Due to using above technical scheme, the utility model has the following beneficial effects:
1) 20-30 test worms can be once tested simultaneously to the behavior reaction of odoring substance to be measured, and working efficiency significantly improves.
2) the care intensity that cold light source incandescent lamp experiences the indoor test worm of activity in all directions always, can effectively be kept away
Exempt from the movement orientation of the differentia influence test worm because of bright and dark light, action selection is made to different odor stimulation to influence test worm.
3) active carbon Filter column, which can effectively remove, mixes aerial background odoring substance, obtains relatively pure air
Stream.
4) on the one hand the first tubaeform connecting tube and the second tubaeform connecting tube increase test worm in recreation room and experience smell
The area of material incentive makes test worm be easier to discriminative stimulus source to induce it to make action selection；Secondly, being trapped into odor source
The test worm of room limits its return recreation room using the small-bore of tubaeform connection tube end, this design can accurately reflect test worm to gas
The behavior reaction of taste substance, experimental result are relatively reliable.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, before not making the creative labor property
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the decomposition texture schematic diagram of the utility model；
Fig. 2 is the assembly structure diagram of utility model.
Specific embodiment
In order to be easy to understand the technical means, creative features, achievement of purpose, and effectiveness of the utility model, under
Face will combine the attached drawing in the utility model embodiment, carry out to the technical scheme in the embodiment of the utility model clear, complete
Ground description.
Embodiment 1
Referring to Fig. 1-2, present embodiment, which adopts the following technical solutions, to be achieved, it includes to be located at miniature air
The outlet side of pump 1, the mini air pump 1 is docked by gas-guide tube with active carbon Filter column 3, and the active carbon Filter column 3 goes out
Gas end is docked by gas-guide tube with gas flowmeter 4, and the outlet side of the gas flowmeter 4 is right by gas-guide tube and water Filter column 6
It connects, the outlet side of the water Filter column 6 is docked by gas-guide tube with threeway 18, and the other both ends of the threeway 18 pass through gas-guide tube row
For test device docking, the performance testing device include the first odor source trapping chamber 8 and the second odor source trapping chamber 20 with
And test worm recreation room 9, the first odor source trapping chamber 8 and the second odor source trapping chamber 20 are symmetricly set on test worm recreation room 9
End, the first odor source trapping chamber 8 docked with test worm recreation room 9 by the first tubaeform connecting tube 10, described second
Odor source trapping chamber 20 is docked by the second tubaeform connecting tube 19 with test worm recreation room 9, the test worm recreation room 9 and the first gas
The end of 20 opposite side of taste source trapping chamber 8 and the second odor source trapping chamber is provided with test worm liberation port 12；
Mini air pump 1, active carbon Filter column 3, gas flowmeter 4, water Filter column 6 and the threeway 18 and behavior
Test device is arranged in light control room bracket 14, and the light control room bracket 14 is generally mutual by bar shaped profile
Splice constituted a cuboid framework structure, bracket 14 upper cover in the light control room is buckled with a floor gobo 17.
The bottom of the gas flowmeter 4 is provided with the gas flowmeter bracket 5 that cooperation is socketed with it.
The bottom setting recreation room bracket 13 of the test worm recreation room 9, the generally rectangular parallelepiped structure of recreation room bracket 13,
Its middle part offers a groove for the insertion of 9 gap of test worm recreation room.
Cold light source white heat 15 and timing control switch 16 are installed on the inner wall of light control room bracket 14.
Embodiment 2
Before experiment, the active carbon filled in active carbon Filter column 3 is first handled to 2 h De contaminations in 220 DEG C of baking oven
After use；When experiment, outside air is compressed in the active carbon Filter column 3 be sent into and be made of active carbon by mini air pump 1 first
Filtering, filtered clean gas flow carry out into one gas by entering water Filter column 6 after the adjusting air flow rate of gas flowmeter 4
The washing impurity-removing of step, the air-flow after second of cleaning enter the first odor source trapping chamber 8 and the second smell of performance testing device
Source trapping chamber 20, and carry respective sample smell in the first odor source trapping chamber 8 and the second odor source trapping chamber 20 and pass through respectively
First tubaeform connecting tube 10 and the second tubaeform connecting tube 19 enter in test worm recreation room 9, then use test worm liberation port 12
The sealing of monolayer medical gauze can be discharged with the mixed gas ensured in test worm recreation room 9.
Test worm introduces at recreation room liberation port 12, and test sample is placed on the first odor source trapping chamber 8, and control sample is put
It sets in the second odor source trapping chamber 20, the odoring substance of test sample volatilization passes through the first loudspeaker from the first odor source trapping chamber 8
Shape connecting tube 10 enters test worm recreation room 9；The odoring substance of reference substance volatilization passes through the second loudspeaker from the second odor source trapping chamber 20
Shape connecting tube 19 enters test worm recreation room 9；
In order to prevent because light unevenly has an impact the action selection of test worm, performance testing device is placed in illumination control
In room bracket 14 processed, control room top surface, two sides and front respectively arrange that the cold light source incandescent lamp tube of 38 W, control outdoor use are black
The processing of color black-out cloth.Test worm generates behavior reaction after experiencing odoring substance: if odoring substance has attracting action to test worm, examination
Worm can enter the first odor source trapping chamber 8 against the tubaeform connecting tube 10 in direction first of olfactory flow；If odoring substance
There is avoidance effect to test worm, test worm can escape Odor stimulation, and the phenomenon that collecting around to test worm liberation port 12 is presented；If smell
Substance does not act on test worm, and test worm will not show the phenomenon that significantly selecting or escaping.This device both can be with blank
Control tests single smell sample to the behavior reaction of test worm, can also behavior reaction of the more different samples to test worm two-by-two.
Experiment is advisable with 6-8 h, after test worm number in recording odor source trapping chamber 8 and for statistical analysis.
It needs thoroughly to clean recreation room 9, the first odor source trapping chamber 8 and the second odor source with dehydrated alcohol before experiment every time and lure
Collect room 20 and all connecting tubes, then wash with distilled water for several times after dried in 50-60 DEG C of baking oven, after being sealed with aluminium-foil paper
It is spare.In order to guarantee the adsorption capacity of active carbon, experiment needs the active carbon after filled high-temperature De contamination every time.
The utility model is designed primarily directed to small-sized Lepidoptera moth class insect, by the stream for changing gas flowmeter 4
The bore of speed and the first tubaeform connecting tube 10 and the second tubaeform connecting tube 19, is suitable for the small-sized moth class elder brother of numerous Lepidopteras
The odorant-binding protein of worm.But not after tested to the behavior reaction of the insects such as other mesh such as coleoptera, Orthoptera, Semiptera,
Action selection effect is unclear
Finally, it should be noted that above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that,
It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc.
With replacement；And these are modified or replaceed, various embodiments of the utility model technology that it does not separate the essence of the corresponding technical solution
The spirit and scope of scheme.
Claims (3)
1. a kind of device of the small-sized moth class insect olfaction behavior of novel test, it is characterised in that it includes to be located at mini air pump
(1), the outlet side of the mini air pump (1) is docked by gas-guide tube with active carbon Filter column (3), the active carbon Filter column
(3) outlet side dock with gas flowmeter (4) by gas-guide tube, the outlet side of the gas flowmeter (4) pass through gas-guide tube and
The outlet side of water Filter column (6) docking, the water Filter column (6) is docked by gas-guide tube with threeway (18), the threeway (18)
In addition both ends are docked by gas-guide tube performance testing device, and the performance testing device includes the first odor source trapping chamber (8)
With the second odor source trapping chamber (20) and test worm recreation room (9), the first odor source trapping chamber (8) lures with the second odor source
Collection room (20) is symmetricly set on the end of test worm recreation room (9), and the first odor source trapping chamber (8) passes through the first tubaeform company
Adapter tube (10) is docked with test worm recreation room (9), and the second odor source trapping chamber (20) passes through the second tubaeform connecting tube (19)
It is docked with test worm recreation room (9), the test worm recreation room (9) and the first odor source trapping chamber (8) and the second odor source trapping chamber
(20) end of opposite side is provided with test worm liberation port (12)；
Mini air pump (1), active carbon Filter column (3), gas flowmeter (4), water Filter column (6) and the threeway (18) with
And performance testing device is arranged in light control room bracket (14), the light control room bracket (14) is generally by item
Shape profile mutually splices constituted a cuboid framework structure, and the light control room bracket (14) upper cover is buckled with floor screening
Light black cloth (17).
2. a kind of device of the small-sized moth class insect olfaction behavior of novel test according to claim 1, it is characterised in that institute
The bottom for stating gas flowmeter (4) is provided with the gas flowmeter bracket (5) that cooperation is socketed with it.
3. a kind of device of the small-sized moth class insect olfaction behavior of novel test according to claim 1, it is characterised in that institute
Recreation room bracket (13) are arranged in the bottom for stating test worm recreation room (9), recreation room bracket (13) the generally rectangular parallelepiped structure, wherein
Portion offers a groove for the insertion of test worm recreation room (9) gap.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821473705.5U CN208925005U (en) | 2018-09-10 | 2018-09-10 | A kind of device of the small-sized moth class insect olfaction behavior of novel test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821473705.5U CN208925005U (en) | 2018-09-10 | 2018-09-10 | A kind of device of the small-sized moth class insect olfaction behavior of novel test |
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| Publication Number | Publication Date |
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| CN208925005U true CN208925005U (en) | 2019-06-04 |
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| CN201821473705.5U Active CN208925005U (en) | 2018-09-10 | 2018-09-10 | A kind of device of the small-sized moth class insect olfaction behavior of novel test |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935345A (en) * | 2018-09-10 | 2018-12-07 | 延安大学 | A kind of device for testing small-sized moth class insect olfaction behavior |
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2018
- 2018-09-10 CN CN201821473705.5U patent/CN208925005U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935345A (en) * | 2018-09-10 | 2018-12-07 | 延安大学 | A kind of device for testing small-sized moth class insect olfaction behavior |
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