CN101766132A

CN101766132A - Test device for memory behavior of aquatic animal

Info

Publication number: CN101766132A
Application number: CN200810208111A
Authority: CN
Inventors: 侯俊利; 庄平; 章龙珍; 张涛; 刘鉴毅; 冯广朋; 赵峰; 黄晓荣; 闫文罡; 冯琳
Original assignee: East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Current assignee: East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Priority date: 2008-12-29
Filing date: 2008-12-29
Publication date: 2010-07-07

Abstract

The invention relates to a test device for the memory behavior of an aquatic animal and solves the technical problem of providing the test device for influence of different water environment factors on the memory behavior of the aquatic animal. The invention adopts a technical scheme that: upper part opened troughs, a light intensity adjustable light source box, a camera, and a PC are adopted; and the PC is provided with software for acquiring and analyzing images and tracking a motion track of the aquatic animal. The test device is characterized in that: four troughs and one light source box are arranged in an outer box, the bottom of each trough is transparent, and the troughs are arranged on the light intensity adjustable light source box; and the camera is arranged at a top opening of the outer box above the four troughs, and video information which is acquired by the camera is transmitted to the PC through a USB data line. The test device is used for testing the memory behavior of the aquatic animal.

Description

The experimental rig of aquatic animal memory behavior

Technical field

The present invention relates to the aquatic animal experimental rig.

Background technology

The whole world much water environments changes or pollutes aggravation, and therefore the mankind extremely pay close attention to the influence of various envirment factors to aquatic animal.Some envirment factors influence nervous system and the memory of aquatic animal.When carrying out envirment factor, animal subject need be placed the tank under the envirment factor conditions of different meterings, observe and the record lively thing memory behavior that tried the water the influence of aquatic animal memory behavior test.This test method exist respectively illumination to animal subject disturb, people's activity to animal subject agitation, different disposal group experimental animal reciprocal effect, writing time difference, workload greatly and on put light source can not good conformity animal behavior analysis software to the open defects such as effective tracking of aquatic animal movement locus.But, do not see the experimental rig of research various dose envirment factor at present both at home and abroad as yet to the influence of aquatic animal memory behavior.

Summary of the invention

The experimental rig that the purpose of this invention is to provide a kind of different water environment factor pair aquatic animal memory behavior influences.

Technical scheme of the present invention adopts tank, the light-source box that can regulate luminous intensity, camera, the PC of opened upper end, PC is installed IMAQ, analysis and aquatic animal movement locus and is followed the tracks of software, it is characterized in that being equipped with 4 tanks and 1 light-source box places among the outer container, bottom of gullet is transparent, and tank places on the light-source box that can regulate luminous intensity; The open top dress camera of the outer container above 4 tanks, the video information of camera collection is sent to PC through the connection of usb data line.

The relative prior art of the present invention has the following advantages and effect: the present invention can be applied to the multiple water environment factor to the test that envirment factor influences the aquatic animal memory behavior, for example salinity, pH value, electric shock intensity, aquatic products medicine, inorganic ions and organic test.The observation of aquatic animal memory behavior in the process of the test, record and analysis can be selected random time point, detection in real time or automatic video recording backup post analysis, thereby realize that envirment factor influences the automatic processing of animal memory behavior test.This envirment factor influences the experimental rig of aquatic animal memory behavior and eliminates the light source difference of prior art and disturb the envirment factor effect of four dosage of analyzing and processing simultaneously.

Description of drawings:

Fig. 1 is the structural representation of experimental rig.

Fig. 2 is the vertical view of experimental tank and a schematic diagram of throwing something and feeding.

Fig. 3 is the plan structure schematic diagram of light-source box.

Fig. 4 is the structural representation of light-source box leading flank.

Fig. 5 is a tank side structure schematic diagram.

Embodiment

The present invention is described in further detail below in conjunction with accompanying drawing, and the composite figure that 4 the tank specifications of the present invention and the back of arranging successively form is fit to IMAQ, analysis and fish movement locus and follows the tracks of the acquisition field of view of software to image.One of optimum specifications of tank 2 (Fig. 1, Fig. 2) is long 30cm * wide 20cm * high 8cm.For avoiding the vision between each tank Mesichthyes to disturb and video acquisition, the side 18 (Fig. 5) of each tank is opaque, transparent, the opened upper end in bottom 17 (Fig. 5).

In order to reduce room natural lighting and people's activity animal subject is disturbed or agitation, outer container 1 (Fig. 1) adopts opaque material to make, the size specification that cooperates above-mentioned tank 2, the main body of outer container 1 is long 65cm * wide 45cm * high 45cm, outer container 1 side is provided with the door that spurs up and down and open and close, and outer container 1 bottom part body is opened and covered on tank 2 and the light-source box 4 (Fig. 1); The top of outer container 1 is taper, the high 15cm of long 65cm * wide 45cm * bore.Camera 5 (Fig. 1) is settled at outer container 1 top, and camera 5 is connected to PC 8 (Fig. 1) by USB mouth video equipment data wire 7 (Fig. 1), and PC is installed animal behavior trajectory analysis software (for example LoliTrack Quatro 1.0).

For described 4 tank 2 (Fig. 1, Fig. 2) daylighting is even, help fish swimming track following software the swimming behavior feature is carried out trace analysis, tank 2 is placed on the light-source box 4 (Fig. 1) that can regulate luminous intensity, the long 65cm of light-source box * wide 45cm * high 15cm by above-mentioned tank specification outfit, open and the arrangement thickness 0.5cm ground glass 3 (Fig. 1 in light-source box 4 tops, Fig. 4), the wall of light-source box and the end 16 (Fig. 4) are opaque, settle 15-25W annular fluorescent lamp 6 (Fig. 1 in the light-source box, Fig. 2, Fig. 3, Fig. 4) illumination, fluorescent lamp 6 is by lead 13 (Fig. 3) polyphone variable resistance 15 (Fig. 3, Fig. 4), adjusting knob 14 (Fig. 3, Fig. 4) with controlling timer 12 (Fig. 3, Fig. 4) regulate the control luminous intensity, make tank 2 (Fig. 1) bottom light intensity be fit to the software track analysis.

With Pb in the test water body ²⁺To the ingest influence of memory behavior of mandarin sturgeon postlarva (average weight 0.2g) is example.In order to reduce the difference of bait throwing in site on intensity of illumination and uniformity coefficient between each tank, set three bait throwing in positions in every tank, respectively according to circular fluorescent lamp radian direction difference in every tank, select 2 at corresponding diagonal angle and central point as the bait throwing in point, centre position 10 (Fig. 2) is for throwing something and feeding at random a little, and the test initial stage throws something and feeds at random in this site; Diagonal position 9,11 (Fig. 2) is respectively the random time point and carries out throwing something and feeding a little of memory training.In order to study of the influence of various dose envirment factor,, add the envirment factor solution of various dose in other 3 tanks at one of 4 tanks dress contrast solution (not containing selected envirment factor) of merely hitting to the fish memory behavior; Envirment factor can be salinity, pH value, electric shock intensity, aquatic products medicine, inorganic ions and organic matter.On-test, in 4 experimental tanks, add Pb respectively ²⁺Concentration is 0,0.2,0.8 and 1.6mg/L solution.Every built-in 9 tail tested fish of tank (mandarin sturgeon postlarva for example, body weight specification 0.35 ± 0.02g).Duration of test by controlling timer 12 (Fig. 3, Fig. 4) control light application time every day be the morning 7:00 to late 19:00.The water silk earthworm of the percentage of liveweight of throwing something and feeding every day 1% gives tested fish (mandarin sturgeon postlarva) as bait.Test first 10d and water silk earthworm is thrown a little 10 (Fig. 2) that throw something and feed at random in each experimental tank at 12:00; At 9:00 water silk earthworm was thrown a little 9 (Fig. 2) that throw something and feed the morning of each experimental tank on the 11st to the 40th day, 19:00 throws a little 11 (Fig. 2) that throw something and feed the afternoon of each experimental tank with water silk earthworm in the afternoon; Do not throw something and feed at the 41st day, but at the 41st day respectively the morning throw something and feed throw something and feed in time and afternoon the time statistical unit in the time tested fish (mandarin sturgeon postlarva) appear at the morning and throw something and feed a little 9 and a little 11 the frequency of throwing something and feeding afternoon, with the movement locus of path of animal movement trace analysis software analysis test fish.

Claims

1. the experimental rig of aquatic animal memory behavior, adopt tank, the light-source box that can regulate luminous intensity, camera, the PC of opened upper end, PC is installed IMAQ, analysis and aquatic animal movement locus and is followed the tracks of software, it is characterized in that being equipped with 4 tanks and 1 light-source box places among the outer container, bottom of gullet is transparent, and tank places on the light-source box that can regulate luminous intensity; The open top dress camera of the outer container above 4 tanks, the video information of camera collection is sent to PC through the connection of usb data line.

2. the experimental rig of aquatic animal memory behavior according to claim 1 is characterized in that the tank specification is long 30cm * wide 20cm * high 8cm.

3. the experimental rig of aquatic animal memory behavior according to claim 1 is characterized in that outer box main body is long 65cm * wide 45cm * high 45cm, and outer box top is taper, the high 15cm of long 65cm * wide 45cm * bore.

4. the experimental rig of aquatic animal memory behavior according to claim 1 is characterized in that the long 65cm of light-source box * wide 45cm * high 15cm, the open and arrangement thickness 0.5cm ground glass in light-source box top.

5. the experimental rig of aquatic animal memory behavior according to claim 1 is characterized in that settling in the light-source box 15-25W annular fluorescent lamp.

6. according to claim 1 or 2 or 3 or the experimental rig of 4 or 5 described aquatic animal memory behaviors, it is characterized in that adding the envirment factor solution of various dose in other 3 tanks at one of 4 tanks dress contrast solution of merely hitting; Envirment factor is salinity or pH value or electric shock intensity or aquatic products medicine or inorganic ions and organic matter.