CN103299944A - Fear memory behavior case for small size aquatic animal - Google Patents
Fear memory behavior case for small size aquatic animal Download PDFInfo
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- CN103299944A CN103299944A CN2013101879825A CN201310187982A CN103299944A CN 103299944 A CN103299944 A CN 103299944A CN 2013101879825 A CN2013101879825 A CN 2013101879825A CN 201310187982 A CN201310187982 A CN 201310187982A CN 103299944 A CN103299944 A CN 103299944A
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- casing
- aquatic animal
- box body
- fear memory
- memory behavior
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a fear memory behavior case for a small size aquatic animal, which comprises a box body and a baffle vertically arranged in the middle of the box body, wherein a strip-shaped passage is reserved between the baffle and the bottom surface of the box body; the baffle divides the box body into a first box body and a second box body; vertical stripes are uniformly arranged on the inner surface of the first box body; horizontal stripes are uniformly arranged on the inner surface of the second box body; and electrodes are arranged on the side surface of the second box body, and are connected with a power supply appliance. The fear memory behavior case has a simple structure, is low in cost and excellent in repeatability of experiment.
Description
Technical field
The present invention relates to the aquatic animal experimental rig, particularly a kind of minitype aquatic animal fear memory behavior case.
Background technology
Minitype aquatic animal such as zebra fish, tadpole, goldfish are widely used in biological study, particularly at research water environment toxin during on the affecting of embryo's nervous system development and cognitive function, often to carry out the learning and memory behavior and detect, but lack at present the experimental rig of studying these minitype aquatic animal learning and memory behaviors.
Summary of the invention
The purpose of this invention is to provide a kind of simple to operate, with low cost, the minitype aquatic animal fear memory behavior case that experimental repeatability is good.
To achieve the above object of the invention, the technical solution used in the present invention is: a kind of minitype aquatic animal fear memory behavior case, comprise casing, vertically be arranged on the dividing plate at described casing middle part, leave strip channel between described dividing plate and described tank floor, described dividing plate is divided into the first casing, the second casing with described casing, described the first interior is equipped with vertical striped, described the second interior is equipped with horizontal stripe, the side of described the second casing is provided with electrode, and described electrode is connected with charger.
Preferred technical scheme, described vertical striped and described horizontal stripe are paster or pad pasting.
Preferred technical scheme, described electrode is provided with two.
Preferred technical scheme, described charger is dc power supply.
The present invention mainly for body length about 3 centimetres, the minitype aquatic animal of being good at moving about.Set up two kinds of different environment in the minitype aquatic animal behaviour area, wherein a kind of environment is to stick chequered with black and white vertical striped in the first interior, another kind of environment is to stick chequered with black and white horizontal stripe in the second interior, the brightness of two kinds of environment is the same, two kinds of environment present in the animal vision is respectively horizontal grating and grating longitudinally, has passage freely to come in and go out for animal between two casings.Then direct current on leading in the second casing, animal training combines the striped of seeing and this fear memory of electric shock.After after a while training, animal is put back to the behavior case, the environment that animal can initiatively be avoided and electric shock combines.
Because technique scheme is used, the present invention compared with prior art has following advantages:
1, the present invention is directed to behavioral characteristic and the habit of minitype aquatic animal, utilize the fear memory behavior of observing minitype aquatic animal to study the water environment toxin to the impact of embryo's nervous system development and cognitive function;
2, the present invention is simple to operate, and is with low cost, and experimental repeatability is good.
Description of drawings
Fig. 1 is structural representation of the present invention;
Fig. 2 is orthogonal view of the present invention.
Wherein: 1, the first casing; 2, the second casing; 3, dividing plate; 4, passage; 5, electrode; 6, dc power supply; 7, vertical striped; 8, horizontal stripe; 9, casing.
Embodiment
The invention will be further described below in conjunction with drawings and Examples:
Embodiment one:
As shown in Figure 1, 2, a kind of minitype aquatic animal fear memory behavior case, comprise casing 9, vertically be arranged on the dividing plate 3 at casing 9 middle parts, leave strip channel 4 between dividing plate 3 and casing 9 bottom surfaces, dividing plate 3 is divided into the first casing 1, the second casing 2 with casing 9, the first casing 1 inner surface is equipped with vertical striped 7, the side that the second casing 2 inner surfaces are equipped with horizontal stripe 8, the second casings 2 is provided with two cube electrodes 5, and electrode 5 is connected with dc power supply 6.
Vertically striped 7 is pad pasting with horizontal stripe 8.
Casing 9 adopts polyethylene to make long 20cm, wide 8.5cm, high 6cm; Dividing plate 3 is opaque polyethylene board; Passage 4 highly is 3cm; Vertically striped 7, horizontal stripe 8 width are 5mm.
The behavior training of minitype aquatic animal fear memory and minitype aquatic animal fear memory behavior case using method:
1, in casing 9, adds water to depth of water 4cm, put into;
2, the observation zebra fish is recorded 10 minutes zebra fishs in the time of staying of two casings in the time of staying of the first casing 1 and the second casing 2, and observed and recorded is put back to fish jar after finishing;
3, installing electrodes 5 in the second casing 2, electrode 5 connects dc power supply 6, dc power supply 6 setting parameters are at 5 volts, single stimulation time is at 100 milliseconds, stimulation mode is continued stimulus, stimulus intervals is 500 milliseconds, zebra fish is put into the first casing 1, switch on immediately whenever zebra fish enters the second casing 2, alarm response can appear in zebra fish, move about fast, flee from electric shock, stop immediately electric shock when zebra fish leaves the second casing 2, through training in 20 minutes, average number of clicks is about 15 times, and training is put back to fish jar with zebra fish after finishing;
4, the electrode in taking-up the second casing 2 is after 5,24 hours, and just zebra fish is put into casing 9, records 10 minutes zebra fishs in the time of staying of the first casing 1 and the second casing 2;
5, calculate rate of avoidance, in rate of avoidance=step 2 zebra fish the second casing 2 the time of staying/step 4 in zebra fish in the time of staying of the second casing 2, resulting rate of avoidance and 100% relatively has significant difference to show that zebra fish has formed fear memory.
The present embodiment is simple to operate, with low cost, experimental repeatability is good, mainly for body length about 3 centimetres, the minitype aquatic animal of being good at moving about, animal training combines the striped of seeing and this fear memory of electric shock, after after a while training, animal is put back to the behavior case, the environment that animal can initiatively be avoided and electric shock combines.
Claims (4)
1. minitype aquatic animal fear memory behavior case, comprise casing, vertically be arranged on the dividing plate at described casing middle part, it is characterized in that: leave strip channel between described dividing plate and described tank floor, described dividing plate is divided into the first casing, the second casing with described casing, and described the first interior is equipped with vertical striped, and described the second interior is equipped with horizontal stripe, the side of described the second casing is provided with electrode, and described electrode is connected with charger.
2. a kind of minitype aquatic animal fear memory behavior case according to claim 1, it is characterized in that: described vertical striped and described horizontal stripe are paster or pad pasting.
3. a kind of minitype aquatic animal fear memory behavior case according to claim 1, it is characterized in that: described electrode is provided with two.
4. a kind of minitype aquatic animal fear memory behavior case according to claim 1, it is characterized in that: described charger is dc power supply.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101879825A CN103299944A (en) | 2013-05-20 | 2013-05-20 | Fear memory behavior case for small size aquatic animal |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101879825A CN103299944A (en) | 2013-05-20 | 2013-05-20 | Fear memory behavior case for small size aquatic animal |
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| CN103299944A true CN103299944A (en) | 2013-09-18 |
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| CN2013101879825A Pending CN103299944A (en) | 2013-05-20 | 2013-05-20 | Fear memory behavior case for small size aquatic animal |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221970A (en) * | 2014-09-25 | 2014-12-24 | 山东省科学院生物研究所 | Device for researching learning and memory ability of zebra fish |
| CN111296329A (en) * | 2020-03-03 | 2020-06-19 | 中国人民解放军第四军医大学 | Zebra fish pain sensation memory and addiction behavior experimental device and method thereof |
| CN111802307A (en) * | 2020-07-29 | 2020-10-23 | 湖南工商大学 | Zebra fish training device |
Citations (2)
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| CN101766132A (en) * | 2008-12-29 | 2010-07-07 | 中国水产科学研究院东海水产研究所 | Test device for memory behavior of aquatic animal |
| CN203226125U (en) * | 2013-05-20 | 2013-10-09 | 苏州大学 | Fear memory behavior box for small-sized aquatic animals |
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2013
- 2013-05-20 CN CN2013101879825A patent/CN103299944A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766132A (en) * | 2008-12-29 | 2010-07-07 | 中国水产科学研究院东海水产研究所 | Test device for memory behavior of aquatic animal |
| CN203226125U (en) * | 2013-05-20 | 2013-10-09 | 苏州大学 | Fear memory behavior box for small-sized aquatic animals |
Non-Patent Citations (7)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104221970A (en) * | 2014-09-25 | 2014-12-24 | 山东省科学院生物研究所 | Device for researching learning and memory ability of zebra fish |
| CN104221970B (en) * | 2014-09-25 | 2016-08-24 | 山东省科学院生物研究所 | A kind of device for studying Brachydanio rerio ability of learning and memory |
| CN111296329A (en) * | 2020-03-03 | 2020-06-19 | 中国人民解放军第四军医大学 | Zebra fish pain sensation memory and addiction behavior experimental device and method thereof |
| CN111802307A (en) * | 2020-07-29 | 2020-10-23 | 湖南工商大学 | Zebra fish training device |
| CN111802307B (en) * | 2020-07-29 | 2022-03-29 | 湖南工商大学 | Zebra fish training device |
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Application publication date: 20130918 |