CN203226125U - Fear memory behavior box for small-sized aquatic animals - Google Patents

Fear memory behavior box for small-sized aquatic animals Download PDF

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Publication number
CN203226125U
CN203226125U CN 201320277846 CN201320277846U CN203226125U CN 203226125 U CN203226125 U CN 203226125U CN 201320277846 CN201320277846 CN 201320277846 CN 201320277846 U CN201320277846 U CN 201320277846U CN 203226125 U CN203226125 U CN 203226125U
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China
Prior art keywords
casing
memory behavior
box body
aquatic animal
electrode
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Expired - Fee Related
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CN 201320277846
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Chinese (zh)
Inventor
车轶
崔勇华
朱玉芳
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Suzhou University
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Suzhou University
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Priority to CN 201320277846 priority Critical patent/CN203226125U/en
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Abstract

The utility model discloses a fear memory behavior box for small-sized aquatic animals. The fear memory behavior box comprises a box body and a clapboard vertically arranged in the middle part of the box body, wherein a strip-type passage is reserved between the clapboard and the bottom surface of the box body, the clapboard divides the box body into a first box body and a second box body, the inner surfaces of the first box body are provided with vertical stripes, the inner surfaces of the second box body are provided with horizontal stripes, the side of the second box body is provided with an electrode, and the electrode is connected with a power supply. The fear memory behavior box disclosed by the utility model is simple in structure, low in cost and good in experiment repeatability.

Description

The frightened memory behavior case of a kind of minitype aquatic animal
Technical field
The utility model relates to the aquatic animal experimental rig, particularly the frightened memory behavior case of a kind of minitype aquatic animal.
Background technology
Minitype aquatic animal such as zebra fish, tadpole, goldfish are widely used in biological study, particularly at research water environment toxin during to the influencing of embryo's nervous system development and cognitive function, often to carry out the learning and memory behavior and detect, but lack the experimental rig of studying these minitype aquatic animal learning and memory behaviors at present.
Summary of the invention
The purpose of this utility model provides a kind of simple to operate, and is with low cost, the frightened memory behavior case of the minitype aquatic animal that experimental repeatability is good.
For achieving the above object, the technical solution adopted in the utility model is: the frightened memory behavior case of a kind of minitype aquatic animal, comprise casing, vertically be arranged on the dividing plate at described casing middle part, leave strip channel between described dividing plate and described tank floor, described dividing plate is divided into first casing, second casing with described casing, described first interior is equipped with vertical striped, described second interior is equipped with horizontal stripe, the side of described second casing is provided with electrode, and described electrode is connected with charger.
Optimized technical scheme, described vertical striped and described horizontal stripe are paster or pad pasting.
Optimized technical scheme, described electrode are provided with two.
Optimized technical scheme, described charger are dc power supply.
The utility model is primarily aimed at body length about 3 centimetres, the minitype aquatic animal of being good at moving about.Set up two kinds of different environment in the minitype aquatic animal behaviour area, wherein a kind of environment is to stick chequered with black and white vertical striped in first interior, another kind of environment is to stick chequered with black and white horizontal stripe in second interior, the brightness of two kinds of environment is the same, two kinds of environment present in the animal vision is respectively horizontal grating and grating longitudinally, has passage freely to come in and go out for animal between two casings.Direct current on leading in second casing then, animal training combines the striped of seeing and this frightened memory of electric shock.After training after a while, animal is put back to the behavior case, the environment that animal can initiatively be avoided and electric shock combines.
Because technique scheme is used, the utility model compared with prior art has following advantage:
1, the utility model utilizes the frightened memory behavior of observing minitype aquatic animal to study the water environment toxin to the influence of embryo's nervous system development and cognitive function at behavioral characteristic and the habit of minitype aquatic animal;
2, the utility model is simple to operate, and is with low cost, and experimental repeatability is good.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the utility model orthogonal view.
Wherein: 1, first casing; 2, second casing; 3, dividing plate; 4, passage; 5, electrode; 6, dc power supply; 7, vertical striped; 8, horizontal stripe; 9, casing.
Embodiment
Below in conjunction with drawings and Examples the utility model is further described:
Embodiment one:
As shown in Figure 1, 2, the frightened memory behavior case of a kind of minitype aquatic animal, comprise casing 9, vertically be arranged on the dividing plate 3 at casing 9 middle parts, leave strip channel 4 between dividing plate 3 and casing 9 bottom surfaces, dividing plate 3 is divided into first casing 1, second casing 2 with casing 9, first casing, 1 inner surface is equipped with vertical striped 7, the side that second casing, 2 inner surfaces are equipped with horizontal stripe 8, the second casings 2 is provided with two cube electrodes 5, and electrode 5 is connected with dc power supply 6.
Vertically striped 7 is pad pasting with horizontal stripe 8.
Casing 9 adopts polyethylene to make long 20cm, wide 8.5cm, high 6cm; Dividing plate 3 is opaque polyethylene board; Passage 4 highly is 3cm; Vertically striped 7, horizontal stripe 8 width are 5mm.
The frightened memory behavior training of minitype aquatic animal and the frightened memory behavior case of minitype aquatic animal using method:
1, in casing 9, adds water to depth of water 4cm, put into;
2, the observation zebra fish is recorded 10 fens clock time zebra fishs in the time of staying of two casings in the time of staying of first casing 1 and second casing 2, and observed and recorded is put back to fish jar after finishing;
3, installing electrodes 5 in second casing 2, electrode 5 connects dc power supply 6, dc power supply 6 parameters are set in 5 volts, single stimulation time is at 100 milliseconds, stimulation mode is continued stimulus, stimulus intervals is 500 milliseconds, zebra fish is put into first casing 1, switch on immediately whenever zebra fish enters second casing 2, alarm response can appear in zebra fish, move about fast, flee from electric shock, stop electric shock immediately when zebra fish leaves second casing 2, through training in 20 minutes, average number of clicks is about 15 times, and training is put back to fish jar with zebra fish after finishing;
4, take out electrode in second casing 2 after 5,24 hours, just zebra fish is put into casing 9, records 10 fens clock time zebra fishs in the time of staying of first casing 1 and second casing 2;
5, calculate rate of avoidance, in rate of avoidance=step 2 zebra fish second casing 2 the time of staying/step 4 in zebra fish in the time of staying of second casing 2, resulting rate of avoidance and 100% relatively has significant difference to show that zebra fish has formed frightened memory.
Present embodiment is simple to operate, with low cost, experimental repeatability is good, be primarily aimed at body length about 3 centimetres, the minitype aquatic animal of being good at moving about, animal training combines the striped of seeing and this frightened memory of electric shock, after training after a while, animal is put back to the behavior case, the environment that animal can initiatively be avoided and electric shock combines.

Claims (4)

1. the frightened memory behavior case of a minitype aquatic animal, comprise casing, vertically be arranged on the dividing plate at described casing middle part, it is characterized in that: leave strip channel between described dividing plate and described tank floor, described dividing plate is divided into first casing, second casing with described casing, and described first interior is equipped with vertical striped, and described second interior is equipped with horizontal stripe, the side of described second casing is provided with electrode, and described electrode is connected with charger.
2. the frightened memory behavior case of a kind of minitype aquatic animal according to claim 1, it is characterized in that: described vertical striped and described horizontal stripe are paster or pad pasting.
3. the frightened memory behavior case of a kind of minitype aquatic animal according to claim 1, it is characterized in that: described electrode is provided with two.
4. the frightened memory behavior case of a kind of minitype aquatic animal according to claim 1, it is characterized in that: described charger is dc power supply.
CN 201320277846 2013-05-20 2013-05-20 Fear memory behavior box for small-sized aquatic animals Expired - Fee Related CN203226125U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201320277846 CN203226125U (en) 2013-05-20 2013-05-20 Fear memory behavior box for small-sized aquatic animals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201320277846 CN203226125U (en) 2013-05-20 2013-05-20 Fear memory behavior box for small-sized aquatic animals

Publications (1)

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CN203226125U true CN203226125U (en) 2013-10-09

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103299944A (en) * 2013-05-20 2013-09-18 苏州大学 Fear memory behavior case for small size aquatic animal
CN105532489A (en) * 2015-12-10 2016-05-04 中国科学院深圳先进技术研究院 Animal experimental device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103299944A (en) * 2013-05-20 2013-09-18 苏州大学 Fear memory behavior case for small size aquatic animal
CN105532489A (en) * 2015-12-10 2016-05-04 中国科学院深圳先进技术研究院 Animal experimental device
CN105532489B (en) * 2015-12-10 2018-12-21 中国科学院深圳先进技术研究院 A kind of animal experiment device

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20131009

Termination date: 20160520