CN203675831U

CN203675831U - Training testing device for mice learning memory ethology

Info

Publication number: CN203675831U
Application number: CN201420009812.8U
Authority: CN
Inventors: 金戈; 张忠; 邹丹; 陈魁敏; 严丹丹; 孙艳; 李晓秀; 殷世亮
Original assignee: Shenyang Medical College
Current assignee: Shenyang Medical College
Priority date: 2014-01-08
Filing date: 2014-01-08
Publication date: 2014-07-02
Anticipated expiration: 2024-01-08

Abstract

The utility model provides a training testing device for the mice learning memory ethology. The problems that an existing device is tedious in operation, prone to being disturbed and single in test item, and therefore experimental data are affected are solved. The device comprises a testing box, a testing bottom plate and a tested object. The training testing device is characterized in that due to the height of the testing box, outside disturbance is avoided, a lighting device provides an appropriate light source for an ethology test, and the testing bottom plate is used for marking and counting in the testing process to facilitate processing of test results. The design of the tested object facilitates experimental error reduction, and operation is convenient for a tester. The device is simple in structure, reasonable in design, convenient to operate and safe and reliable in using, one device can be used for multiple experiments, the accuracy of the experimental results is guaranteed, an experimental animal is effectively prevented from being disturbed, and material resources are saved.

Description

Learning and memory of little mouse behaviouristics training test device

Technical field

The utility model relates to a kind of animal medicine experimental provision that is applicable to central nervous system pathology model, particularly a kind of learning and memory of little mouse behaviouristics training test device for the research such as neuro-physiology, Neuropharmacology.Be mainly used in the diagnosis of central nervous system pathology model and the screening of medicine drug effect.

Background technology

Animal Behavior Science is to specialize in a science of animal behavior, has correlation with multiple subjects.Along with scientific and technical development, the medical experiment of Animal Behavior Science has become the indispensable important means of research higher brain function, has critical role in the research field such as neuro-physiology, Neuropharmacology.In traditional Animal Behavior Science medical experiment task, need them how to do as the animal of can informing of pathological model experiment itself.In research process, can be divided into reference memory and working memory.Animal, in training test process, first needs device by experiment to complete reference memory, and it has reflected the study (such as spatial cues position, experimental rules etc.) of animal to each test phase relevant information in task.Therefore, animal, forming in the process of reference memory, should be present in each experimentation with reference to information.In training test process, the working memory of animal refers in each conditioned reflex test, retains the ability of information between different examinations is inferior.Laboratory animal after an examination is inferior can wipe current specific working memory, but keeps reference memory constant.Therefore working memory only works in once testing, and reference memory belongs to long-term memory.At present, for aspect learning and memory behavior training test devices such as neuro-physiology, Neuropharmacology, the general test that adopts simple test box to carry out individual event, and carry out manual record, artificial disturbance many factors, reliable experiment result is low, can not reach the object that desirable behavior training is tested.

Existing training test device and existing problems: often adopt easy wooden box.This device is all generally uncovered test casing, and general size is 50 × 50 × 15cm(length × wide × height).It can not avoid extraneous interference, and laboratory animal has the situation of the casing that jumps out.In addition, because researcher needs color, the shape of alternative condition reflection measurement object voluntarily, between the different examination of each conditioned reflex test is inferior, can not repeat to present the content of learning and memory behavior, exist subjective limitation.Also without unified regulation, therefore there is certain human factor, bring deviation to experimental result at aspects such as manual record experimental datas.Therefore, learn the actual needs of training test model according to setting up animal learning memory behavior, develop a learning and memory behaviouristics experimental provision multi-functional, easy and simple to handle for improving quality of experiments, reduce experimenter's labour intensity important in inhibiting.

Utility model content

The purpose of this utility model is to provide a kind of learning and memory of little mouse behaviouristics training test device, solve the subjective limitation that exists in existing training test device, can not repeat to present the problems such as learning and memory content of the act, its specification for structure, reasonable in design, easy to operate, safe and reliable, meet multinomial learning and memory behaviouristics test request, further improve the true and reliable property of test data.

The technical scheme that the utility model adopts is: this learning and memory of little mouse behaviouristics training test device comprises uncovered test casing and puts into the test object of casing, it is characterized in that: described casing is of a size of length × wide × height and is not less than 50 × 50 × 30cm, light source is set in casing, and the test object of putting into casing adopts two cylindrical body that color is identical, size shape is identical or two color differences, cylindrical body that size shape is different.

The described test object of putting into casing is replaced by and adopts a tape square graduated base.

thisthe advantage that utility model has and positive technique effect are: be not less than 50 × 50 × 30cm because the uncovered casing of the utility model employing is of a size of length × wide × height, light source is set in casing and highly increases, not only avoid the laboratory animal casing that jumps out, and can effectively avoid the interference of extraneous sound and light, so its specification for structure is reasonable in design, easy to operate, safe and reliable, further improve the true and reliable property of test data.In addition, put into the selectivity variation of the test object that causes conditioned reflex of casing, can utilize the congenital principle that new test object is had to the tendency explored of animal, the learning and memory testing arrangement of setting up has the feature that allows laboratory animal carry out action learning memory training test under free movement state, more approx the learning and memory behavior of simulating human.By the conversion to new test object (as size shape, color etc.), be conducive to the formation of experiment test animal short-term or long-term memory mechanism and the impact judgement that medicine forms memory in moment, thereby further improve the true and reliable property of data.

Accompanying drawing explanation

Below in conjunction with accompanying drawing, the utility model is further described.

Fig. 1 is a kind of structural representation that in the utility model test casing, test object is cylindrical body;

Fig. 2 is the cutaway view of Fig. 1 along A-A line;

Fig. 3 is that in the utility model test casing, test object is a kind of structural representation of tape square graduated base.

Fig. 4 is the cutaway view of Fig. 1 along B-B line.

Sequence number explanation in figure: 1 casing, 2 cylindrical body, 3 light sources, 4 tape square graduated bases.

Embodiment

Describe concrete structure of the present utility model in detail according to Fig. 1～4.This learning and memory of little mouse behaviouristics training test device comprises uncovered test casing 1 and puts into the parts such as the test object of casing 1.Wherein casing 1 is of a size of length × wide × height and is not less than 50 × 50 × 30cm, above the sidewall in casing 1 or casing 1, light source 3 is set.Compared with existing traditional casing, this casing 1 is interior arranges light source 3 and highly increase, not only avoids the laboratory animal casing 1 that jumps out, and can effectively avoid the interference of extraneous sound and light.Putting into the test object of casing 1 can select according to the actual needs of learning and memory of little mouse behaviouristics training test, both can adopt two cylindrical body that color is identical, size shape is identical 2 or two color differences, cylindrical body 2 that size shape is different.Also can according to actual needs the test object for cylindrical body 2 of putting into casing 1 be replaced by and adopt a tape square graduated base 4.

Mouse carries out three learning and memory behaviouristics and trains the concrete operation step of test as follows:

Two cylindrical body that color is identical, size shape is identical 2 of the present embodiment employing are A, B, C totally three groups of test objects of a group.A group in three groups of test objects is two red square cylinders, and B group is two yellow cylinders, and C group is two green hexagonal prisms.The sidewall configuration 10W illuminating lamp of test casing 1 is as light source 3.

One, learning and memory behaviouristics test phase

1, the phase that conforms is put into test casing 1 in the current period by experimental mouse, makes experimental mouse freely movable.Adapt to two days continuously, 10 minutes every days (being the laundering period in behaviouristics).

2, the 3rd day training period, was positioned over experimental mouse separately in test casing 1, put into A(or B or C) group test object, allow experimental mouse freely explore, each 10 minutes.For the reference memory of training experiment mouse.

3, the testing period after training finishes 1 hour by one in the group of A wherein test object one of changing in B group test object, experimental mouse is put into test casing 1 again, two test object time used and number of times explored in record.The investigation time is similarly 10 minutes.After 24h, then B is organized to of changing in C test object in test object.

For realizing automatic control, in the top of test casing 1 configuration camera (not shown).Explore number of times and time by camera record.Data, after related software is processed, are analyzed and are shown result.Tri-groups of cylindrical body of A, B, C that test object is made up of different colours and shape, have avoided test mouse affected by color and shape hobby, bring deviation to experiment, and the test object of these three groups of shapes is easy to be positioned in test casing 1 and tests.This test is investigated for ability of learning and memory.

Two, social interaction behaviouristics test phase

1, conform: laboratory animal is conformed separately 2 days, each 10 minutes.

2, test phase: select two test experiments mouse, be divided into and initiatively test mouse and Passive Test mouse, the same sex mouse that certain choosing is waited upon from different mouse cages.Two test mouse are distinguished by different marks.Two test mouse are put into test casing 1 simultaneously, and emphasis records frequency of exposure and the time of initiatively testing mouse.Passive Test mouse carries out experimental data statistics as reference.This test is for contacts and adaptive mensuration.

Three, open environmental behaviour is learned test phase

When test, tape square graduated base 4 is put into test casing 1 with the one side of scale.Experimental mouse is put on the tape square graduated base 4 of test casing 1, allows it freely explore, and the time is 10 minutes.Numeration experimental mouse, at the movable grid number of periphery and central authorities, is analyzed experimental mouse and whether is had the state of mind such as anxiety, anxiety.Square scale on base plate can be used for mark and the counting in experimentation.The moods such as scale is for detection of whether existential anxiety of experimental mouse, nervous.This test experiments mouse enters the various actions after open environment.

The present invention is for diagnosis and the curative effect of medication screening of central nervous system pathology model.According to behaviouristics related data, carry out interpretation of result and data statistics draws experiment conclusion through software.

Claims

1. a learning and memory of little mouse behaviouristics training test device, comprise uncovered test casing and put into the test object of casing, it is characterized in that: described casing is of a size of length × wide × height and is not less than 50 × 50 × 30cm, light source is set in casing, and the test object of putting into casing adopts two cylindrical body that color is identical, size shape is identical or two color differences, cylindrical body that size shape is different.

2. learning and memory of little mouse behaviouristics training test device according to claim 1, is characterized in that: described in put into casing test object be replaced by and adopt a tape square graduated base.