CN108935333A - The method of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm - Google Patents

The method of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm Download PDF

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Publication number
CN108935333A
CN108935333A CN201810637443.XA CN201810637443A CN108935333A CN 108935333 A CN108935333 A CN 108935333A CN 201810637443 A CN201810637443 A CN 201810637443A CN 108935333 A CN108935333 A CN 108935333A
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China
Prior art keywords
bee
card
trichogramma
bollworm
protectiveness
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CN108935333B (en
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宋凯
王玉波
李和平
李积铭
方美娟
何晓庆
刘冬
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Dry Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences
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Dry Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants

Abstract

The present invention relates to the methods of a kind of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm; include: that (i) production mixes the long-acting bee card stage: (1) making protectiveness bee card; protectiveness bee blocks the brown paper production of a face hair side one side smooth surface, and every protectiveness bee card includes 10~100 single bee cards.It (2) is that host breeds Trichogramma chilonis and Trichogramma Ostriniae respectively with gelechiid ova;(3) bee card is prepared, prepares bee card in two batches;Uniformly mixed parasitic wasp is uniformly sprinkling upon on card when preparation bee card.(ii) it puts the bee stage: (1) putting the bee time, first is in summer corn ear period are as follows: summer corn ear period, corn borer, which sprouts wings, to be started to contain fashionable;Second batch and first bee card interval discharge for 5 days.(2) bee method is put, and per hectare corn field of summer hangs 150 single bee cards, puts bee point apart from 4~5m of the edge of a field, put between bee point 7~9m meters of distance.The present invention, which can be reduced, puts bee number, effectively increases trichogramma in the effect of the time-to-live in field and prevention and treatment corn borer and bollworm.

Description

The method of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm
Technical field
The invention belongs to agricultural pests technical field of biological control, are related to a kind of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer With the method for bollworm.
Background technique
Corn borer and bollworm are the important grain and cotton pests in China, and in China, the Yellow River and Huai He River sea region is made on corn, cotton throughout the year At serious harm.In recent years, as Cotton in China main producing region is shifted, bollworm is on the rise in corn field harm, in Huang-Huai-Hai Summer corn growing area, corn borer and bollworm have become the most important pest of summer corn ear period.In the control of insect process of many years In, China has developed the technical method of a variety of prevention and treatment corn borers and bollworm, wherein discharging its parasitics day in the pest egg-laying season Opposing trichogramma is technical method more effective and the most environmentally protective in all multi-methods.The Yellow River and Huai He River sea region important grain in China's is made The biological prevention of primary pest corn borer and bollworm on object summer corn.
Currently, China has been successfully realized with a variety of trichogrammas of vector large-scale breeding, wherein Trichogramma Ostriniae It is the sociales parasitic wasp of the Yellow River and Huai He River sea area corn borer and bollworm respectively with Trichogramma chilonis.Since trichogramma is to target pest ovum Parasitism have certain obligate, it is limited to the control effect of another non-target insects to discharge single honeybee kind, in addition the Yellow River and Huai He River sea region Bollworm lags compared with corn borer peak period throughout the year, therefore conventional method prevents and treats two kinds of pests and needs to discharge two kinds of trichogrammas respectively, It causes field adaptability number, put the increase of the bee amount of labour used, increase control of insect cost.Bee card on do not sprout wings bee be easy by ladybug, The predators feeding such as Chrysopa, reduces the quantity of bee, affects the effect for preventing pest.
Summary of the invention
The object of the present invention is to provide the methods of a kind of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm, solve yellow The insect pest problem of Huai-Hai region summer corn ear period corn borer and bollworm, with reduce put bee number, protect bee card on bee not by Natural enemy predation improves the effect of mixing trichogramma prevention and treatment corn borer and bollworm.
The technical scheme is that the method for release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm, including preparation It mixes long-acting bee card and puts two stages of bee, the specific steps are as follows:
Production mixes long-acting bee card
(1) production protectiveness bee card, protectiveness bee block a face hair side, the brown paper production of another side smooth surface, every guarantor Shield property bee card includes 10~100 single bee cards, and paper quality is 150~200g/m2.The smooth surface of brown paper carry out printing template and Card is cut, template includes gluing position, fold line, notch, trapezoid-shaped openings and perforation line.The middle part cross cutting of protectiveness bee card extrudes wide by 2 ~3mm fold line, it is convenient for folding.It is die cut out perforation line between single bee card, to facilitate the tearing off.Single bee blocks to be cut out close to top one end Notch apart from each 0.5~1.0cm in both sides, single bee card bottom two corners respectively have a trapezoid-shaped openings, and the trapezoidal protrusion of formation is used for It is inserted into notch.The gluing on the hair side under bee card fold line, glue sites are placed in the middle, 5 ± 0.5cm2 of every card gluing, coated face with from The covering of type paper;
It (2) is that host breeds Trichogramma chilonis and Trichogramma Ostriniae respectively with gelechiid ova with laying off eggs breeding bee method;
(3) preparation bee card, prepares bee card in two batches, each single bee card of first bee card containing Trichogramma Ostriniae 1000 ± 200, Trichogramma chilonis 500 ± 100, the every card of second batch bee card contains Trichogramma Ostriniae 500 ± 100, Trichogramma chilonis 1000 ± 200.The Trichogramma Ostriniae and Trichogramma chilonis that every batch of uses include it is parasitic after at 25 DEG C~28 DEG C development 2~ 3 days, 3~4 days, 4~5 days and 5~6 days parasitic wasps account for 30%, 20%, 20% and 30% respectively;Bee is removed when preparation bee card Uniformly mixed parasitic wasp is uniformly sprinkling upon on card, sweeps off the parasitic wasp not being sticked in glue surface with banister brush by the release paper on card.
(ii) bee is put
(1) the bee time is put, the field adaptability time of first bee card are as follows: summer corn ear period, corn borer emergence start to contain emerging When;The release time of second batch bee card and first bee card are spaced 5 days, i.e., discharge within the 6th day after the card release of first bee;
(2) bee place and quantity are put, bee is put by the amount that per hectare corn field of summer hangs 150 bees card, puts bee point apart from the edge of a field 4 ~5m, putting distance between bee point is 7~9m.
Alternatively, the specification of brown paper used in long-term effect protectiveness bee is 35cm × 14cm, each protectiveness bee Ka Bao Block containing 20 bees, single bee card size is 3.5cm × 7cm.Notch close to top one end 0.6cm, notch apart from each 0.7cm in both sides, The long 0.6cm in the upper bottom edge of trapezoid-shaped openings, go to the bottom side length 0.8cm, high 0.6cm.The glue of gluing is based on oiliness acrylic adhesive Want the oiliness non-setting adhesive cement of composition.The application method of protectiveness bee card are as follows: single bee card tears it down when putting bee, bee blocks from folding Line position folds, and by the trapezoidal protrusion partial insertion top cut away of bee card bottom, is placed in parasitic wasp between two layers of paper jam, by bee Jam (slotting) is in the blade lower part close to corncob position.
The present invention provides two kinds of honeybee kinds different to target pest efficiency of mixing to be discharged, while preventing and treating summer corn fringe Phase corn borer and bollworm.Bollworm is prevented and treated in application Trichogramma Ostriniae prevention and treatment corn field corn borer, using Trichogramma chilonis On the basis of, occur the phase in conjunction with the ovum of Huang-Huai-Hai summer corn ear period corn borer and bollworm and closes on, and peak period area Other actual conditions are discharged simultaneously by mixing honeybee kind and put bee number to reduce field, difference bee when by clearly putting bee every time The burst size of kind meets the needs of field different target control of insect.The trichogramma that the present invention uses includes being bred with gelechiid ova Ostrinia furnacalis trichogramma Trichogramma ostriniae Pang et Chen and Trichogramma chilonis Trichogramma Chilonis Ishii can be used for the Yellow River and Huai He River sea region corn borer, the corn field of summer that bollworm mixing occurs.
The present invention discharges the method for mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm according to the Yellow River and Huai He River sea area summer corn ear period Corn borer and bollworm time of origin feature and trichogramma law of development change the bee card and method for releasing of trichogramma Into, can be reduced and put bee number, while protect bee block on bee do not preyed on by natural enemy, improve mixing trichogramma prevention and treatment corn borer and The effect of bollworm.
Detailed description of the invention
Fig. 1 is the expanded schematic diagram of protectiveness bee card;
Fig. 2 is the inhibiting effect effect picture that trichogramma endangers corn borer;
Fig. 3 is the inhibiting effect effect picture that trichogramma endangers bollworm;
Wherein: 1-gluing position, 2-notch, 3-mono- bee cards, 4-fold lines, 5-perforation lines, 6-trapezoid-shaped openings, 7- Trapezoidal protrusion.
Specific embodiment
Below with reference to embodiment and attached drawing, the present invention is described in detail.The scope of protection of the present invention is not limited to the embodiment, Those skilled in the art make any change within the scope of the claims and also belong to the scope of protection of the invention.
The method that the present invention discharges mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm, including preparation mix long-acting bee card and Put two stages of bee, the specific steps are as follows:
Preparation mixes long-acting bee card
(1) production protectiveness bee card, protectiveness bee block a face hair side, the brown paper production of another side smooth surface, Mei Gebao Shield property bee card includes 10 single bee cards 3, and the specification of quality of paper used is 150g/m2, and the smooth surface of brown paper carries out printing template With cut card, template includes gluing position 1, fold line 4, notch 2, trapezoid-shaped openings 6 and perforation line 5.Protectiveness bee blocks brown paper used Specification be 35cm × 14cm (contain 20 single deck tape-recorders), each protectiveness bee card includes 20 single bees cards, and single bee card size is 3.5cm ×7cm.The middle part cross cutting of protectiveness bee card extrudes wide 2~3mm fold line 4, convenient for folding, is die cut out perforation between single bee card 3 Line 5 is to facilitate the tearing off.Single bee, which blocks, cuts out the notch 2 apart from each 0.7cm in both sides close to top one end, and notch 2 is close to top one end 0.6cm.Single bee card bottom two corners respectively have a trapezoid-shaped openings, and the long 0.6cm in the upper bottom edge of trapezoid-shaped openings 6, go to the bottom side length 0.8cm, High 0.6cm, the trapezoidal protrusion of formation is for being inserted into notch.The gluing on the hair side under single bee card fold line, glue sites are placed in the middle, 5 ± 1cm2 of every card gluing, coated face are covered with release paper.The glue of gluing is oiliness acrylic adhesive oiliness as the main component Non-setting adhesive cement;
It (2) is that host breeds Trichogramma chilonis and Trichogramma Ostriniae respectively with gelechiid ova with laying off eggs breeding bee method;
(3) preparation bee card prepares bee card in two batches.1. making the bee card of first release, when fabrication, takes 2~3d of development, 3 The Trichogramma Ostriniae of~4d, 4~5d and 5~6d each 4,500,000,3,000,000,3,000,000 and 4,500,000, Trichogramma chilonis each 2,250,000, 1500000,1,500,000 and 2,250,000.Two kinds of bees are fabricated into after mixing on protectiveness bee card, each list bee card 3 contains 1500 bees. The bee made is stuck in 20 DEG C~25 DEG C of temperature, stores or distributes under conditions of relative humidity 75%~85%.2. second batch is released The bee card put, when fabrication, take the Trichogramma Ostriniae of 2~3d of development, 3~4d, 4~5d and 5~6d each 2,250,000,1,500,000,150 Ten thousand and 2,250,000, Trichogramma chilonis each 4,500,000,3,000,000,3,000,000 and 4,500,000.Two kinds of bees are fabricated into protectiveness after mixing On bee card, every list bee card contains 1500 bees.The bee made is stuck in 20 DEG C~25 DEG C of temperature, under the conditions of relative humidity 75%~85% Storage or distribution.The release paper on single bee card is removed when preparing single bee card, and uniformly mixed parasitic wasp is uniformly sprinkling upon on card, is used Banister brush sweeps off the extra parasitic wasp not being sticked in glue surface.
(ii) bee is put
(1) single bee card 3 is torn it down when putting bee, single bee card is folded from fold line position, by the trapezoidal protrusion 7 of bee card bottom Partial insertion top cut away 2 is placed in parasitic wasp between two layers of paper jam, and before the dusk for putting the bee same day, bee jam (slotting) is being leaned on The blade lower part of nearly corncob position.
(2) the bee time is put, the field adaptability time of first single bee card are as follows: summer corn ear period, corn borer sprout wings and start to contain Fashionable;The release time of second batch list bee card and first single bee card are spaced 5 days, i.e., the 6th day after first single bee card release Release;
(3) bee quantity and place are put, bee is put by the amount that per hectare corn field of summer hangs 150 single bee cards, puts bee point distance ground First 4~5m, putting distance between bee point is 8.2m.
Implementation result:
2017 in the Gucheng County village Di Kou, the Jing County Modern Agricultural Park Jin Long and Jing County Zhi Qing agricultural cooperative society, using this The method prevention and treatment corn borer and bollworm of invention release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm.Table 1 the result shows that, lead to Trichogramma releases is crossed, trichogramma reaches 90% or more to corn borer pieces of an egg parasitic rate, is significantly higher than the prior art (control CK) 55%, ovum grain parasitic rate reaches 80% or more, and pest decline rate reaches 70% or more.To the investigation knot of field corn snout moth's larva harm For fruit as shown in Fig. 2, showing that trichogramma releases is significant to corn borer inhibiting effect, it is killed than (right that trichogramma prevents and treats corn borer field According to CK) reduce by 70% or more.
1 trichogramma of table prevents and treats corn borer parasitic rate
Table 2 the result shows that, after the trichogramma releases of cotton field, the area Fang Feng trichogramma to bollworm eggs parasitic rate reach 80% with On, it is significantly higher than the 34.78% of (control CK), preventive effect reaches 75% or more.To investigation result such as Fig. 3 of Helicoverpa armigera harm It is shown, show that trichogramma releases is obvious to bollworm inhibiting effect, it is killed than (control CK) drop that trichogramma prevents and treats Helicoverpa armigera Low 65% or more.
2 trichogramma of table prevents and treats bollworm parasitic rate
Compared with conventional method, the present invention reduces prevention and treatment bollworm and puts bee 2 times, and the total dosage of trichogramma reduces by 10,000/mu, Reducing prevents insect pest and agriculture production cost.
The glue that the present invention uses is oiliness acrylic adhesive, there is gelechiid ova with good bond performance, Gu ovum is imitated Fruit is good, and nontoxic, tasteless, there is no starch material water suction dehydration is fast, the problem of influencing bee in ovum.Bee can mention before being stuck in use It is preceding largely to make spare, a large amount of artificial fabrication bring places of concentration and recruitment problems during being avoided that trichogramma production.Protection Property bee be stuck in during field adaptability and complete final step and fold plug-in card work, increase adhesion process without additional.Trichogramma exists Between two layers of paper jam, due to cross cutting when on card extrude folding line position, it is folded two layers card between there are reserving gaps, can hinder The insect predators such as gear ladybug, Chrysopa enter feeding trichogramma, and cute conjunctivitis bee imago enters and leaves enough, without punching on bee blocks.

Claims (4)

1. a kind of method of release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm, it is characterized in that: the method includes preparations It mixes long-acting bee card and puts two stages of bee, the specific steps are as follows:
(i) the long-acting bee card of mixing is prepared
(1) production protectiveness bee card, the protectiveness bee block a face hair side, the brown paper production of another side smooth surface, every guarantor Shield property bee card includes 10~100 single bee cards (3);The smooth surface printing template and Qie Ka of the brown paper, the template include applying Glue position (1), fold line (4), notch (2), trapezoid-shaped openings (6) and perforation line (5), the middle part of the protectiveness bee card, which is die cut, to be extruded Wide 2~3mm fold line (4), single bee blocks to be die cut out perforation line (5) between (3), and single bee, which blocks, to be cut out close to top one end apart from both sides The notch (2) of each 0.5~1.0cm, single bee card bottom two corners respectively have a trapezoid-shaped openings (6);Hair under single bee card fold line Gluing on face, glue sites are placed in the middle, and 5 ± 0.5cm2 of every card gluing, coated face is covered with release paper;
It (2) is that host breeds Trichogramma chilonis and Trichogramma Ostriniae respectively with gelechiid ova with laying off eggs breeding bee method;
(3) preparation bee card, prepares bee card in two batches, first bee blocks (3) each single bee card and contains Trichogramma Ostriniae 1000 ± 200 Head, Trichogramma chilonis 500 ± 100, each card of second batch bee card contains Trichogramma Ostriniae 500 ± 100, Trichogramma chilonis 1000 ± 200;The Trichogramma Ostriniae and Trichogramma chilonis that every batch of uses include it is parasitic after at 25 DEG C~28 DEG C development 2~ 3 days, 3~4 days, 4~5 days and 5~6 days parasitic wasps account for 30%, 20%, 20% and 30% respectively;Bee is removed when preparation bee card Uniformly mixed parasitic wasp is uniformly sprinkling upon on paper jam, is swept off with banister brush and be not sticked to posting in glue surface by the release paper on paper jam Raw bee;
(ii) bee is put
(1) the bee time is put, first bee is in summer corn ear period are as follows: summer corn ear period, corn borer, which sprouts wings, to be started to contain fashionable;Second batch The release time of bee card and first single bee card are spaced 5 days.
(2) bee place and quantity are put, puts bee by the amount that per hectare corn field of summer hangs 150 single bees cards, put bee point apart from the edge of a field 4~ 5m, putting distance between bee point is 7~9m.
2. the method for release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm according to claim 1, it is characterized in that: institute The specification for stating brown paper used in long-term effect protectiveness bee is 35cm × 14cm, and each protectiveness bee card includes 20 single bee cards (3), The list bee card size is 3.5cm × 7cm;The notch (2) close to top one end 0.6cm, notch apart from each 0.7cm in both sides, The long 0.6cm in upper bottom edge of trapezoid-shaped openings (6), go to the bottom side length 0.8cm, high 0.6cm.
3. the method for release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm according to claim 1, it is characterized in that: institute The glue for stating gluing is oiliness acrylic adhesive oiliness non-setting adhesive cement as the main component.
4. the method for release mixing cute conjunctivitis honeybee kind prevention and treatment corn borer and bollworm according to claim 1, it is characterized in that: institute State the application method of protectiveness bee card are as follows: single bee card (3) tears it down when putting bee, single bee card is folded from fold line position, by bee Trapezoidal protrusion (7) partial insertion top cut away (2) of card bottom, is placed in parasitic wasp between two layers of paper jam, bee jam is being leaned on The blade lower part of nearly corncob position.
CN201810637443.XA 2018-06-20 2018-06-20 Method for preventing and controlling corn borers and cotton bollworms by releasing mixed trichogramma seeds Active CN108935333B (en)

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CN109769754A (en) * 2019-03-22 2019-05-21 广东省农业科学院植物保护研究所 A kind of breeding release card, folded breeding release card and application method
CN111011313A (en) * 2019-12-30 2020-04-17 中国医学科学院药用植物研究所云南分所 Method for improving female proportion of trichogramma offspring and application thereof
CN111820055A (en) * 2020-08-12 2020-10-27 河北全疆科技有限公司 Automatic trichogramma egg card putting device and method for putting trichogramma egg card by using same
CN111937655A (en) * 2020-08-20 2020-11-17 河北省农林科学院旱作农业研究所 Row bee card for unmanned aerial vehicle release, manufacturing method and using method
CN111820055B (en) * 2020-08-12 2024-05-07 河北全疆科技有限公司 Automatic trichogramma egg card throwing device and trichogramma egg card throwing method using same

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CN105766803A (en) * 2016-03-15 2016-07-20 吉林农业大学 Method for blended breeding of trichogramma dendrolimi and trichogramma ostriniae with oak silkworm eggs as hosts
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109769754A (en) * 2019-03-22 2019-05-21 广东省农业科学院植物保护研究所 A kind of breeding release card, folded breeding release card and application method
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CN111011313B (en) * 2019-12-30 2022-04-19 中国医学科学院药用植物研究所云南分所 Method for improving female proportion of trichogramma offspring and application thereof
CN111820055A (en) * 2020-08-12 2020-10-27 河北全疆科技有限公司 Automatic trichogramma egg card putting device and method for putting trichogramma egg card by using same
CN111820055B (en) * 2020-08-12 2024-05-07 河北全疆科技有限公司 Automatic trichogramma egg card throwing device and trichogramma egg card throwing method using same
CN111937655A (en) * 2020-08-20 2020-11-17 河北省农林科学院旱作农业研究所 Row bee card for unmanned aerial vehicle release, manufacturing method and using method

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