CN108934909A - A kind of processing method of the waste generated with cassava fermentation alcohol - Google Patents
A kind of processing method of the waste generated with cassava fermentation alcohol Download PDFInfo
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Abstract
The invention belongs to environmental protection technical fields; a kind of processing method of waste generated with cassava fermentation alcohol is specifically disclosed; specific step is as follows: step 1: carrying out waste cassava residues addition white oil and zeolite to be ground into jelly; then mixing is adjusted into liquid and mixed microorganism is added; stewing process after mixing obtains processing slurry；Step 2: mixing, which is added, in useless mash adjusts liquid and mixed bacteria liquid, room temperature is stood after stirring, obtains handling useless mash；Step 3: discarding molasses, fresh soya bean shell, filter mud, pig manure, edible fungi residues and being uniformly mixed processing slurry, the useless mash of processing and sugarcane, build heap fermentation, it is primary at interval of turning in 10 days, control moisture content is 50-55%, stop fermentation after fermentation 30 days and obtain cultivation matrix A, then cultivation matrix B can be obtained in the moisture content of processing cultivation matrix A to 45%.The advantages of the application: can turn waste into wealth, and additionally provide a beneficial processing method, greatly improve the economic interests of fermentation plant.
Description
[technical field]
The invention belongs to environmental protection technical fields, and in particular to a kind of place of the waste generated with cassava fermentation alcohol
Reason method.
[background technique]
Alcohol is also known as ethyl alcohol, has many uses, and may be utilized in fabricating acetic acid, beverage, essence, dyestuff, fuel etc., medically
The alcohol that common volume fraction is 70%-75% makees disinfectant.Alcohol Production is had gradually developed on the basis of wine brewing,
Alcohol product can be divided into potato alcohol, corn alcohol, molasses-spirit, biomass alcohol etc. according to raw materials for production difference, and alcohol produces
Product are divided into superfine alcohol, high grade edible spirit, universal alcohol, industrial alcohol according to quality is different again.In recent years, with to grain
Understanding of importance constantly enhances, and the non-grain cassava alcohol yield market share expands year by year.The raw material packet that cassava alcohol produces and uses
Include: cassava, amylase, carbohydrase, distiller's yeast, saccharifying agent, technique are obtained after the crushing of cassava, boiling, saccharification, fermentation, distillation
Finished product alcohol；Mature wine with dregs is obtained after zymotechnique, the ingredient of mature wine with dregs is the mixing of alcohol and carbon dioxide and a small amount of impurity
Object, the solid filtered out after distilling is vinasse, liquid is alcohol, gas part.A large amount of waste, cassava are generated after manufacture
Waste residue and useless mash, its two inappropriate processing will cause the pollution of soil and water quality, thus reasonable processing waste cassava residues and
Useless mash and using being extremely urgent problem.
[summary of the invention]
Goal of the invention of the invention is: the object of the present invention is to provide a kind of wastes generated with cassava fermentation alcohol
Processing method, can not only be turned waste into wealth by the processing method of the application, additionally provide a beneficial processing method, significantly
The economic interests for improving fermentation plant.
To achieve the goals above, The technical solution adopted by the invention is as follows:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added by body, stewing process 20-30 hours after mixing, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 20-30:2-5:1-3；
The additional amount that the mixing adjusts liquid is the 10%-20% of jelly total weight；
The mixed microorganism is by aspergillus oryzae, candida valida, aureobasidium pullulans, pseudomonas putida, mould
Bacterium is that 1-2:1-4:2-3:1-2:1-3 mixture forms according to volume ratio；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 40-50 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 50-80:3-5:5-9；
The mixed bacteria liquid by aureobasidium pullulans, pseudomonas putida, Penicillium notatum, mine-laying silk branch is mould is according to volume ratio
2-5:1-3:1-5:2-4 mixture forms；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
50-55% stops fermentation after fermentation 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can obtain
To cultivation matrix B,
Wherein, processing slurry 5%-15%, raw material handles useless mash by being mixed according to the proportion of percent by weight
5%-10%, sugarcane discard molasses 5%-10%, fresh soya bean shell 15%-30%, filter mud 15%-20%, pig manure 15%-
30%, edible fungi residues 10%-15%；
In the first step and second step, the mixing adjusts liquid and is formed by following raw material according to weight percent mixture:
25%-35% platform money grass extracting solution, 20%-30% Salvia japonica extracting solution, 20%-30% Trichodesma calycosum extracting solution, 15%-20% maos
Fruit grass extracting solution.
It further illustrates, the extracting solution that the mixing adjusts constitutive material in liquid is prepared by following methods, specifically
Steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 10-20 times of volume, is extracted controlled at soxhlet extraction at 50 DEG C
20-30 minutes, extracting solution is taken, takes supernatant after revolution is 2000-2500r/min centrifuge centrifugal treating 20-30s；
S2 depigmentation: supernatant is toppled over for the speed of 2-3ml/s along absorption column wall with flow velocity, collects outflow absorption
The clear liquid of column；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 2-3ml/s speed by clear liquid along the suction for being mounted with D284 weak-base anion-exchange resin
Attached column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse lye and eluted, collect eluent, can be obtained
Extracting solution.
Preferably, the hydroxide that the potassium hydroxide solution and mass concentration that the lye is 15% by mass concentration are 25%
Sodium solution is mixed according to volume ratio for 1:2-3.
Further illustrate, strain is all made of activated from preservation state by corresponding culture medium after use, wherein
The culture medium of the aspergillus oryzae is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 2.5g/L,
Banana puree 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000m L, pH to 6.8；
The culture medium of the candida valida is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone
1.0g/L, tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 7.2；
The culture medium of the aureobasidium pullulans is prepared from the following raw materials: apple polysaccharide 10.0g/L, ammonium sulfate 0.07g/L,
Sodium chloride 0.1g/L, epsom salt 0.05g/L, potassium dihydrogen phosphate 1.05g/L, deionized water 500m L, pH to 4.8；
The culture medium of the pseudomonas putida is prepared from the following raw materials: apple polysaccharide 15.0g/L, corn pulp 5.0g/L,
Sodium dihydrogen phosphate 0.15g/L, epsom salt 0.10g/L, calcium chloride 0.5g/L, deionized water 1000m L, pH to 7.3；
The culture medium of the Penicillium notatum is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.5g/L, chlorine
Change sodium 2.0g/L, corn pulp 3.5g/L, deionized water 1000m L, pH to 7.0；
The mould culture medium of the mine-laying silk branch is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.0g/
L, potassium chloride 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000m L, pH to 6.5.
It further illustrates, the effective bacteria containing amount of aspergillus oryzae is 1.03 × 108-2.11×108CFU/mL；
The effective bacteria containing amount of candida valida is 1.11 × 108-2.46×108CFU/mL；
The effective bacteria containing amount of aureobasidium pullulans is 0.89 × 108-1.56×108CFU/mL；
The effective bacteria containing amount of pseudomonas putida is 1.12 × 108-2.35×108CFU/mL；
The effective bacteria containing amount of Penicillium notatum is 1.07 × 108-2.43×108CFU/mL；
The mould effective bacteria containing amount of mine-laying silk branch is 0.78 × 108-1.28×108CFU/mL。
It further illustrates, the filter mud is beet-sugar factory sulfitation sugar mill filter mud；The filter mud carbon-nitrogen ratio is 30-40:
1。
A kind of processing method of waste generated with cassava fermentation alcohol as described in claim 1, cultivation matrix B are answered
For cultivating rich and honour dish.
All raw materials of the application can be purchased in market and be arrived.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
The application in biomass to alcohol conversion process waste cassava residues and useless mash carry out after reasonably handling, difference can reduce wood
COD and BOD5 content in potato waste residue and useless mash, and the cyanide content in waste cassava residues of degrading, and be able to maintain and meeting state
The cultivation matrix obtained after fermentation process is carried out in the range of family's regulation with other raw materials, and cultivation matrix is exclusively for my ground
The rich and honour dish configuration just abounded with, so that it is in water retention property, the physicochemical properties such as organic composition content adapt to its growth, improve rich
The quality of your dish.
[specific embodiment]
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the invention
The mode of applying is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But
The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hair
Similar improvement is done in the case where bright intension, therefore the present invention is not limited to the specific embodiments disclosed below.
Strain is all made of activated from preservation state by corresponding culture medium after use, wherein
The culture medium of the aspergillus oryzae is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 2.5g/L,
Banana puree 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000m L, pH to 6.8；
The culture medium of the candida valida is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone
1.0g/L, tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 7.2；
The culture medium of the aureobasidium pullulans is prepared from the following raw materials: apple polysaccharide 10.0g/L, ammonium sulfate 0.07g/L,
Sodium chloride 0.1g/L, epsom salt 0.05g/L, potassium dihydrogen phosphate 1.05g/L, deionized water 500m L, pH to 4.8；
The culture medium of the pseudomonas putida is prepared from the following raw materials: apple polysaccharide 15.0g/L, corn pulp 5.0g/L,
Sodium dihydrogen phosphate 0.15g/L, epsom salt 0.10g/L, calcium chloride 0.5g/L, deionized water 1000m L, pH to 7.3；
The culture medium of the Penicillium notatum is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.5g/L, chlorine
Change sodium 2.0g/L, corn pulp 3.5g/L, deionized water 1000m L, pH to 7.0；
The mould culture medium of the mine-laying silk branch is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.0g/
L, potassium chloride 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000m L, pH to 6.5.
Being understood that by the condition of adjusting activation culture and the time of activation can be obtained by the bacterium after above-mentioned activation
The bacterium of different effective bacteria containing amounts.
Embodiment 1:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 20 hours by body, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 20:2:1；
The additional amount that the mixing adjusts liquid is the 10% of jelly total weight；
The mixed microorganism is 1.03 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzae, effective bacteria containing amount are 1.11
×108CFU/mL candida valida, effective bacteria containing amount are 0.89 × 108CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.12× 108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.07 × 108CFU/mL Penicillium notatum is 1:1 according to volume ratio:
2:1:1 mixture forms；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 40 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 50:3:5；
The mixed bacteria liquid is 0.89 × 10 by effective bacteria containing amount8CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.12×108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.07 × 108CFU/mL Penicillium notatum, effective bacteria containing amount are
0.78×108CFU/m mine-laying silk branch is mould to be formed according to volume ratio for 2:1:1:2 mixture；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
Stop fermentation after 50%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution that mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 10 times of volumes, is extracted 20 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, takes supernatant after revolution is 2000r/min centrifuge centrifugal treating 20s；
S2 depigmentation: supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 2ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resin
Column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is that 1:2 is mixed and made into lye and is eluted according to volume ratio, collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to cultivate rich and honour dish.
Comparative example 1: processing scheme is substantially same as Example 1, difference be in the first step waste cassava residues without white oil
With zeolite milled processed.
Comparative example 2: processing scheme is substantially same as Example 1, and difference is that waste cassava residues are not added with white oil in the first step
It is ground.
Comparative example 3: processing scheme is substantially same as Example 1, and difference is that waste cassava residues are not added with zeolite in the first step
It is ground.
Verification test one:
Waste cassava residues after producing alcohol as raw material using cassava in comparative example 1-3, embodiment 1 are subjected to cyanide content
Detection, former content is 4.62 ± 0.31mg/kg, after milled processed process in the first step in comparative example 1-3, embodiment 1,
The content containing cyanide in waste cassava residues is detected, Parallel testing three times is done in detection every time, is averaged；Detection
The result is as follows:
Table 1
As seen from the above table, using the technical solution of the application, the content for the cyanide that can greatly degrade compares and does not adopt
The method degradation rate variation of this technology processing less, and greatly improves after illustrating the technical solution compounding of the application, mentions
High rate is at least up to 40%.This is by the applicant the study found that being ground by white oil and zeolite and waste cassava residues, white
Oil can be crosslinking in during the grinding process in the cyanide in waste cassava residues, make cyanide in the environment of grinding generates heat
Three keys fracture, to degrade the content of cyanide in waste cassava residues；And the addition of zeolite promotes and increases fever in grinding, energy
The efficiency for quickly improving fracture, further promotes the degradation of cyanide.
By the waste cassava residues after producing alcohol as raw material using cassava in comparative example 1-3, embodiment 1 carry out COD value, BOD5 contains
The detection of amount, COD original content are 325.36 ± 12.67mg/kg, and BOD5 original content is 99.84 ± 18.64mg/kg, using comparison
In the first step in example 1-3, embodiment 1 after milled processed process, COD value, the BOD5 content in waste cassava residues are detected,
Parallel testing three times is done in detection every time, is averaged；Detection the result is as follows:
Table 2
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
The method degradation rate variation for this technology processing not used less, and mentions significantly after illustrating the technical solution compounding of the application
It is high.This is by the applicant the study found that being ground by white oil and zeolite and waste cassava residues, and white oil is during the grinding process
It can be embedded in the structure of waste cassava residues, aoxidize COD, BOD5 in waste cassava residues and divide
Solution, this researcher also found can be crosslinked by process of lapping white oil with zeolite, improved and changed zeolite structured cavernous structure
It is intensive more intensively to form honeycomb, greatly improves the ability of CO absorption D, BOD5 of zeolite；This is used by also demonstrating in table
The compounding raw material and proportion of application are than the excellent effect of single use；The degradation rate of COD of comparing at least improves 27%, BOD5
Degradation rate at least improve 18%.
Embodiment 2:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 30 hours by body, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 30:5:3；
The additional amount that the mixing adjusts liquid is the 20% of jelly total weight；
The mixed microorganism is 2.11 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzae, effective bacteria containing amount are 2.46
×108CFU/mL candida valida, effective bacteria containing amount are 1.56 × 108CFU/mL aureobasidium pullulans, effective bacteria containing amount are
2.35× 108CFU/mL pseudomonas putida, effective bacteria containing amount are 2.43 × 108CFU/mL Penicillium notatum is 2:4 according to volume ratio:
3:2:3 mixture forms；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 50 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 80:5:9；
The mixed bacteria liquid is 1.56 × 10 by effective bacteria containing amount8CFU/mL aureobasidium pullulans, effective bacteria containing amount are
2.35×108CFU/mL pseudomonas putida, effective bacteria containing amount are 2.43 × 108CFU/mL Penicillium notatum, effective bacteria containing amount are
1.28×108CFU/m mine-laying silk branch is mould to be formed according to volume ratio for 5:3:5:4 mixture；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
Stop fermentation after 55%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution that mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 20 times of volumes, is extracted 30 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, takes supernatant after revolution is 2500r/min centrifuge centrifugal treating 30s；
S2 depigmentation: supernatant is toppled over for the speed of 3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resin
Column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is that 1:3 is mixed and made into lye and is eluted according to volume ratio, collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to cultivate rich and honour dish.
Contrast groups 1: processing scheme is substantially same as Example 1, difference be in the first step waste cassava residues without mixing
Microbial fermentation processing.
Contrast groups 2: processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of aspergillus oryzae.
Contrast groups 3: processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of candida valida.
Contrast groups 4: processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of aureobasidium pullulans.
Contrast groups 5: processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of pseudomonas putida.
Contrast groups 6: processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of Penicillium notatum.
Verification test two:
By the waste cassava residues after producing alcohol as raw material using cassava in contrast groups 1-6, embodiment 2 carry out COD value, BOD5 contains
The detection of amount, COD original content are 311.34 ± 21.21mg/kg, and BOD5 original content is 98.25 ± 12.07mg/kg, by grinding
COD original content is 111.23 ± 14.31mg/kg after processing, BOD5 original content is 35.67 ± 5.64mg/kg, using contrast groups
In the first step in 1-6, embodiment 2 after fermentation treatments, COD value, the BOD5 content in waste cassava residues are detected, often
Parallel testing three times is done in secondary detection, is averaged；Detection the result is as follows:
Table 3
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) the degradation rate variation for this technology processing not used less, and illustrates the technical solution compounding of the application
After substantially increase degradation rate.Degradation rate after compounding as seen from the table improves at least one compared to the degradation rate of single effect
Half, have the function that synergy, the applicant's research is the discovery that because inherently containing some harmful bacterias in waste cassava residues,
In the environment of harmful bacteria exists so that COD, BOD5 content in waste cassava residues slowly increase, by the prescription after compounding from
The destructions such as the growth carbon source of different bacterium, PH, oxygen amount are so that harmful bacteria stress stops growing or is destroyed, the prescription after compounding
In can greatly COD, BOD5 content increase and can absorption degradations always.
Embodiment 3:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 25 hours by body, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 22:3:2；
The additional amount that the mixing adjusts liquid is the 13% of jelly total weight；
The mixed microorganism is 1.57 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzae, effective bacteria containing amount are 1.64
×108CFU/mL candida valida, effective bacteria containing amount are 0.99 × 108CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.73× 108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.64 × 108CFU/mL Penicillium notatum is 1:2 according to volume ratio:
3:2:2 mixture forms；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 44 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 60:4:7；
The mixed bacteria liquid is 1.02 × 10 by effective bacteria containing amount8CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.57×108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.81 × 108CFU/mL Penicillium notatum, effective bacteria containing amount are
0.89×108CFU/m mine-laying silk branch is mould to be formed according to volume ratio for 3:2:2:3 mixture；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
Stop fermentation after 52%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution that mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 13 times of volumes, is extracted 27 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, takes supernatant after revolution is 2000r/min centrifuge centrifugal treating 25s；
S2 depigmentation: supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resin
Column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is that 1:2 is mixed and made into lye and is eluted according to volume ratio, collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to cultivate rich and honour dish.
Control group 1: processing scheme is substantially same as Example 3, difference be in the first step waste cassava residues without mixing
Adjust liquid fermentation process.
Control group 2: processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of platform money grass extracting solution.
Control group 3: processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of Salvia japonica extracting solution.
Control group 4: processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of Trichodesma calycosum extracting solution.
Control group 5: processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of comospore grass extracting solution.
Verification test three:
By the waste cassava residues after producing alcohol as raw material using cassava in control group 1-5, embodiment 3 carry out COD value, BOD5 contains
The detection of amount, COD original content are 312.36 ± 20.04mg/kg, and BOD5 original content is 97.69 ± 20.31mg/kg, by grinding
COD original content is 102.35 ± 11.21mg/kg after processing, BOD5 original content is 31.32 ± 8.34mg/kg, using contrast groups
In the first step in 1-5, embodiment 3 after fermentation treatments, COD value, the BOD5 content in waste cassava residues are detected, often
Parallel testing three times is done in secondary detection, is averaged；Detection the result is as follows:
Table 4
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) degradation rate for this technology processing not used is told somebody what one's real intentions are, and is illustrated big after the technical solution of the application compounds
Degradation rate is improved greatly.Degradation rate after compounding as seen from the table improves at least half compared to the degradation rate of single effect, reaches
The effect of synergy is arrived, the applicant's research is the discovery that in the adjusting liquid after compounding the organic acid containing there are many, in containing for organic acid
Under the action of amount and degradation can be greatly improved after microbial action, the organic acid of the hobby of each microorganism is different
Sample, situation after compounding has a service life for the extended activity microorganism for greatly promoting microorganism, specific mechanism each
The deviation of the specific organic acid of microorganism hobby is to be studied.
Embodiment 4:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 28 hours by body, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 27:4:2；
The additional amount that the mixing adjusts liquid is the 14% of jelly total weight；
The mixed microorganism is 1.86 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzae, effective bacteria containing amount are 1.89
×108CFU/mL candida valida, effective bacteria containing amount are 1.21 × 108CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.94× 108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.52 × 108CFU/mL Penicillium notatum is 2:3 according to volume ratio:
3:2:1 mixture forms；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 46 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 70:4:8；
The mixed bacteria liquid is 1.19 × 10 by effective bacteria containing amount8CFU/mL aureobasidium pullulans, effective bacteria containing amount are
1.69×108CFU/mL pseudomonas putida, effective bacteria containing amount are 1.32 × 108CFU/mL Penicillium notatum, effective bacteria containing amount are
1.10×108CFU/m mine-laying silk branch is mould to be formed according to volume ratio for 4:2:3:2 mixture；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
Stop fermentation after 54%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, the extracting solution that mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 16 times of volumes, is extracted 25 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, takes supernatant after revolution is 2500r/min centrifuge centrifugal treating 26s；
S2 depigmentation: supernatant is toppled over for the speed of 3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resin
Column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is that 1:3 is mixed and made into lye and is eluted according to volume ratio, collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to cultivate rich and honour dish.
Control 1: processing scheme is substantially same as Example 4, and difference is that useless mash processing is not added with mixing in second step
Adjust liquid fermentation process.
Control 2: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without platform money grass extracting solution.
Control 3: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Salvia japonica extracting solution.
Control 4: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Trichodesma calycosum extracting solution.
Control 5: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without comospore grass extracting solution.
Control 6: processing scheme is substantially same as Example 4, and difference is that useless mash processing is not added with mixing in second step
Bacterium solution fermentation process.
Control 7: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without aureobasidium pullulans.
Control 8: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without pseudomonas putida.
Control 9: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Penicillium notatum.
Control 10: processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
It is mould without mine-laying silk branch.
Verification test four:
1-10 will be compareed, in embodiment 4 using useless mash progress COD value, BOD5 content that cassava is after raw material produces alcohol
Detection, COD original content be 986.36 ± 65.34mg/kg, BOD5 original content be 215.36 ± 28.64mg/kg, to using pair
According to 1-10, embodiment 4 technology after COD value, the BOD5 content in useless mash are detected, detection is done parallel three times every time
Detection, is averaged；Detection the result is as follows:
Table 4
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) degradation rate for this technology processing not used is told somebody what one's real intentions are, and is illustrated big after the technical solution of the application compounds
Degradation rate is improved greatly.It is better than comparing the degradation rate of 1-10 using the degradation rate of the technical solution of embodiment 4 as seen from the table,
And wherein the minimum and combined mixing adjusting liquid of the unmixed degradation rate for adjusting liquid and unmixed bacterium solution and mixed bacteria liquid are equal
It is more excellent than the degradation effect of single component.
Embodiment 5:
A kind of processing method of the waste generated with cassava fermentation alcohol, the specific steps are as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out grinding pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 28 hours by body, obtains processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 27:4:2；
The additional amount that the mixing adjusts liquid is the 17% of jelly total weight；
The mixed microorganism is 2.07 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzae, effective bacteria containing amount are 2.15
×108CFU/mL candida valida, effective bacteria containing amount are 1.34 × 108CFU/mL aureobasidium pullulans, effective bacteria containing amount are
2.09× 108CFU/mL pseudomonas putida, effective bacteria containing amount are 2.11 × 108CFU/mL Penicillium notatum is 1:3 according to volume ratio:
2:1:2 mixture forms；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 48 hours afterwards, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 76:4:7；
The mixed bacteria liquid is 1.34 × 10 by effective bacteria containing amount8CFU/mL aureobasidium pullulans, effective bacteria containing amount are
2.08×108CFU/mL pseudomonas putida, effective bacteria containing amount are 2.09 × 108CFU/mL Penicillium notatum, effective bacteria containing amount are
1.07×108CFU/m mine-laying silk branch is mould to be formed according to volume ratio for 4:1:3:3 mixture；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new
Cadmium yellow beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
Stop fermentation after 53%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, the extracting solution that mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 15 times of volumes, is extracted 28 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, takes supernatant after revolution is 2500r/min centrifuge centrifugal treating 28s；
S2 depigmentation: supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 2ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resin
Column removes efflux；
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is that 1:3 is mixed and made into lye and is eluted according to volume ratio, collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to cultivate rich and honour dish.
Test proves five:
The cultivation matrix that embodiment 1-5 is prepared plants rich and honour dish, and planting patterns is using conventional plantation
Environment and sprinkling method；
General planting method plants rich and honour dish using common cultivation matrix；
Cultivation matrix and common cultivation matrix are subjected to composition detection and physicochemical property detection；After the harvesting of rich and honour dish, and
Nutritional ingredient in rich and honour dish is detected；Specific detection table is as follows:
5 cultivation matrix physical property of table and common cultivation matrix contrast table
| Classification | Total porosity/% | Water-holding porosity/% |
| Embodiment 1 | 82.01 | 55.14 |
| Embodiment 2 | 81.32 | 54.35 |
| Embodiment 3 | 82.35 | 54.25 |
| Embodiment 4 | 83.24 | 5.36 |
| Embodiment 5 | 82.35 | 57.25 |
| Common cultivation matrix | 65.46 | 46.84 |
6 cultivation matrix chemical property of table and common cultivation matrix contrast table
By upper table 5,6 it is found that using the physicochemical property of the application treated cultivation matrix than common cultivation matrix
Physicochemical property is excellent.
7 soilless cultivation sweet potato leaves nutritional ingredient of table and common cultivation substrate culture wealth and rank dish contrast table unit: mg/100g
As seen from the above table, the cultivation matrix prepared using the application can be improved rich and honour dish for cultivating rich and honour dish
The comparision contents of potassium element vitamin B, C and P elements are obvious, improve the quality of rich and honour dish.
Following table is respectively the proportion of constituent and percentage meter in embodiment 1-5:
8 matrix of table forms the proportion table according to percent by weight
Mixing adjusts liquid according to the proportion table of percent by weight in 9 first step of table and second step
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Platform money grass extracting solution | 31% | 35% | 30% | 32% | 25% |
| Comospore grass extracting solution | 20% | 15% | 20% | 18% | 19% |
| Salvia japonica extracting solution | 22% | 20% | 30% | 25% | 29% |
| Trichodesma calycosum extracting solution | 27% | 30% | 20% | 25% | 27% |
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of processing method of the waste generated with cassava fermentation alcohol, which is characterized in that specific step is as follows:
Step 1: the waste cassava residues addition white oil and zeolite after producing alcohol as raw material using cassava carry out being ground into jelly,
Then mixing is adjusted into liquid and mixed microorganism is added, stewing process 20-30 hours after mixing, obtain processing slurry；
The weight ratio of the waste cassava residues, white oil and zeolite is 20-30:2-5:1-3；
The additional amount that the mixing adjusts liquid is the 10%-20% of jelly total weight；
The mixed microorganism is pressed by aspergillus oryzae, candida valida, aureobasidium pullulans, pseudomonas putida, Penicillium notatum
It is that 1-2:1-4:2-3:1-2:1-3 mixture forms according to volume ratio；
Step 2: mixing, which is added, in the useless mash after producing alcohol as raw material using cassava adjusts liquid and mixed bacteria liquid, after stirring often
Temperature stands 40-50 hours, obtains handling useless mash；
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 50-80:3-5:5-9；
It according to volume ratio is 2-5 that the mixed bacteria liquid is mould by aureobasidium pullulans, pseudomonas putida, Penicillium notatum, mine-laying silk branch:
1-3:1-5:2-4 mixture forms；
Step 3: the processing that processing slurry that the first step obtains, second step are obtained is given up, mash and sugarcane discard molasses, new cadmium yellow
Beans shell, filter mud, pig manure, edible fungi residues are uniformly mixed, and build heap fermentation, and primary at interval of turning in 10 days, control moisture content is 50-
Stop fermentation after 55%, fermentation 30 days and obtain cultivation matrix A, then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, processing slurry 5%-15%, raw material handles useless mash 5%- by being mixed according to the proportion of percent by weight
10%, sugarcane discards molasses 5%-10%, fresh soya bean shell 15%-30%, filter mud 15%-20%, pig manure 15%-30%, food
With bacteria residue 10%-15%；
In the first step and second step, the mixing adjusts liquid and is formed by following raw material according to weight percent mixture: 25%-
35% grass extracting solution, 20%-30% Salvia japonica extracting solution, 20%-30% Trichodesma calycosum extracting solution, 15%-20% comospore grass
Extracting solution.
2. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 1, it is characterised in that:
The extracting solution that the mixing adjusts constitutive material in liquid is prepared by following methods, the specific steps are as follows:
S1 is extracted: being added raw materials into the distilled water of 10-20 times of volume, is extracted 20-30 controlled at soxhlet extraction at 50 DEG C
Minute, extracting solution is taken, takes supernatant after revolution is 2000-2500r/min centrifuge centrifugal treating 20-30s；
S2 depigmentation: supernatant is toppled over for the speed of 2-3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid；Deacidite D101 absorption resin is mounted in the adsorption column；
S3, with flow velocity for 2-3ml/s speed by clear liquid along the adsorption column for being mounted with D284 weak-base anion-exchange resin,
Remove efflux；
S4, using the adsorption column in distilled water flushing S3, reuse lye and eluted, collect eluent, extraction can be obtained
Liquid.
3. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 2, it is characterised in that:
The sodium hydroxide solution that the potassium hydroxide solution and mass concentration that the lye is 15% by mass concentration are 25% is according to volume
Than being mixed for 1:2-3.
4. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 1, it is characterised in that:
Strain is all made of activated from preservation state by corresponding culture medium after use, wherein
The culture medium of the aspergillus oryzae is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 2.5g/L, banana
Mud 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000mL, pH to 6.8；
The culture medium of the candida valida is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.0g/L,
Tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000mL, pH to 7.2；
The culture medium of the aureobasidium pullulans is prepared from the following raw materials: apple polysaccharide 10.0g/L, ammonium sulfate 0.07g/L, chlorination
Sodium 0.1g/L, epsom salt 0.05g/L, potassium dihydrogen phosphate 1.05g/L, deionized water 500mL, pH to 4.8；
The culture medium of the pseudomonas putida is prepared from the following raw materials: apple polysaccharide 15.0g/L, corn pulp 5.0g/L, phosphoric acid
Sodium dihydrogen 0.15g/L, epsom salt 0.10g/L, calcium chloride 0.5g/L, deionized water 1000mL, pH to 7.3；
The culture medium of the Penicillium notatum is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.5g/L, sodium chloride
2.0g/L, corn pulp 3.5g/L, deionized water 1000mL, pH to 7.0；
The mould culture medium of the mine-laying silk branch is prepared from the following raw materials: apple polysaccharide 10.0g/L, soy peptone 1.0g/L, chlorine
Change potassium 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000mL, pH to 6.5.
5. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 1, it is characterised in that:
The effective bacteria containing amount of aspergillus oryzae is 1.03 × 108-2.11×108CFU/mL；
The effective bacteria containing amount of candida valida is 1.11 × 108-2.46×108CFU/mL；
The effective bacteria containing amount of aureobasidium pullulans is 0.89 × 108-1.56×108CFU/mL；
The effective bacteria containing amount of pseudomonas putida is 1.12 × 108-2.35×108CFU/mL；
The effective bacteria containing amount of Penicillium notatum is 1.07 × 108-2.43×108CFU/mL；
The mould effective bacteria containing amount of mine-laying silk branch is 0.78 × 108-1.28×108CFU/mL。
6. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 1, it is characterised in that:
The filter mud is beet-sugar factory sulfitation sugar mill filter mud；The filter mud carbon-nitrogen ratio is 30-40:1.
7. a kind of processing method of waste generated with cassava fermentation alcohol according to claim 1, it is characterised in that:
Cultivation matrix B is applied to cultivate rich and honour dish.
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