CN108713474A - A kind of processing method of alcohol waste - Google Patents
A kind of processing method of alcohol waste Download PDFInfo
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- CN108713474A CN108713474A CN201810397716.8A CN201810397716A CN108713474A CN 108713474 A CN108713474 A CN 108713474A CN 201810397716 A CN201810397716 A CN 201810397716A CN 108713474 A CN108713474 A CN 108713474A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention belongs to environmental protection technical fields, specifically disclose a kind of processing method of alcohol waste, are as follows:The first step:White oil is added in waste cassava residues and zeolite is ground pulp body, mixing is then adjusted into liquid and mixed microorganism is added, stewing process after mixing, obtains processing slurry;Second step:Mixing is added in useless mash and adjusts liquid and mixed bacteria liquid, room temperature is stood after stirring, obtains handling useless mash;Third walks:Processing slurry, the useless mash of processing and the discarded molasses of sugarcane, fresh soya bean shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, build heap fermentation, it is primary at interval of turning in 10 days, control moisture content is 50-55%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained cultivation matrix B.The advantages of the application:It can turn waste into wealth, additionally provide a beneficial processing method, greatly improve the economic interests of fermentation plant.
Description
【Technical field】
The invention belongs to environmental protection technical fields, and in particular to a kind of processing method of alcohol waste.
【Background technology】
Alcohol is also known as ethyl alcohol, has many uses, and may be utilized in fabricating acetic acid, beverage, essence, dyestuff, fuel etc., medically
The alcohol that common volume fraction is 70%-75% makees disinfectant.Alcohol Production has gradually developed on the basis of wine brewing,
Alcohol product can be divided into potato alcohol, corn alcohol, molasses-spirit, biomass alcohol etc., alcohol production according to raw materials for production difference
Product are divided into according to quality difference as superfine alcohol, high grade edible spirit, universal alcohol, industrial alcohol.In recent years, with to grain
Understanding of importance constantly enhances, and the non-grain cassava alcohol yield market share expands year by year.The raw material packet that cassava alcohol produces and uses
It includes:Cassava, amylase, carbohydrase, distiller's yeast, saccharifying agent, technique are obtained after the crushing of cassava, boiling, saccharification, fermentation, distillation
Finished product alcohol;Ripe wine with dregs is obtained after zymotechnique, the ingredient of ripe wine with dregs is the mixing of alcohol and carbon dioxide and a small amount of impurity
Object, the solid filtered out after distillation is vinasse, liquid is alcohol, gas part.A large amount of waste, cassava are generated after manufacture
Waste residue and useless mash, its two inappropriate processing can cause the pollution of soil and water quality, thus rational processing waste cassava residues and
Useless mash and using being extremely urgent problem.
【Invention content】
The goal of the invention of the present invention is:The object of the present invention is to provide a kind of processing methods of alcohol waste, pass through
The processing method of the application can not only turn waste into wealth, and additionally provide a beneficial processing method, greatly improve fermentation plant
Economic interests.
To achieve the goals above, the technical solution adopted by the present invention is as follows:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added by body, stewing process 20-30 hours after mixing, obtains processing slurry;
The weight ratio of the waste cassava residues, white oil and zeolite is 20-30:2-5:1-3;
The addition that the mixing adjusts liquid is the 10%-20% of jelly total weight;
The mixed microorganism is pressed by aspergillus oryzae, candida valida, Aspergillus flavus, bacillus licheniformis, Penicillium notatum
It is 1-2 according to volume ratio:1-3:1-4:2-3:2-4 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 40-50 hours afterwards, obtains handling useless mash;
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 50-80:3-5:5-9;
It according to volume ratio is 1- that the mixed bacteria liquid is mould by Aspergillus flavus, bacillus licheniformis, Penicillium notatum, mine-laying silk branch
3:2-3:1-3:2-4 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, and primary at interval of turning in 10 days, control moisture content is 50-
55%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, raw material according to the proportioning of percent by weight by being mixed, processing slurry 5%-15%, the useless mash of processing
5%-10%, sugarcane discard molasses 5%-10%, fresh soya bean shell 15%-30%, filter mud 15%-20%, cow dung 15%-
30%, boiler ash sediment 10%-15%;
In the first step and second step, the mixing adjusts liquid and is formed according to weight percent mixture by following raw material:
25%-35% long stamen bothriospermum chinenses extracting solution, 20%-30% Chinese herbaceous peonies shikonin extract, the micro- fruit grass extracting solutions of 20%-30%, 15%-
20% purple perilla extracting solution.
It further illustrates, the extracting solution that the mixing adjusts constitutive material in liquid is prepared by following methods, specifically
Steps are as follows:
S1 is extracted:The distilled water for adding raw materials into 10-20 times of volume extracts controlled at soxhlet extraction at 50 DEG C
20-30 minutes, extracting solution is taken, is to take supernatant after 2000-2500r/min centrifuges handle 20-30s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 2-3ml/s along absorption column wall with flow velocity, collects outflow absorption
The clear liquid of column;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 2-3ml/s speed by clear liquid along the suction for being mounted with D284 weak-base anion-exchange resins
Attached column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse lye and eluted, collect eluent, you can obtain
Extracting solution.
Preferably, the hydroxide that the potassium hydroxide solution and mass concentration that the lye is 15% by mass concentration are 25%
Sodium solution is 1 according to volume ratio:2-3 is mixed.
Further illustrate, strain is all made of activated from preservation state by corresponding culture medium after use, wherein
The culture medium of the aspergillus oryzae is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 2.5g/L,
Banana puree 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000m L, pH to 6.8;
The culture medium of the candida valida is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone
1.0g/L, tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 7.2;
The culture medium of the Aspergillus flavus is made of following raw material:Apple polysaccharide 10.0g/L, agar 2.0g/L, potassium chloride
2.0g/L, epsom salt 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 6.2;
The culture medium of the bacillus licheniformis is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone
1.0g/L, sodium chloride 2.0g/L, epsom salt 3.0g/L, ferrous sulfate 0.02g/L, deionized water 1000m L, pH to 7.0;
The culture medium of the Penicillium notatum is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.5g/L, chlorine
Change sodium 2.0g/L, corn steep liquor 3.5g/L, deionized water 1000m L, pH to 7.0;
The mould culture medium of the mine-laying silk branch is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.0g/
L, potassium chloride 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000m L, pH to 6.5.
It further illustrates, the effective bacteria containing amount of aspergillus oryzae is 1.03 × 108-2.11×108CFU/mL;
The effective bacteria containing amount of candida valida is 1.11 × 108-2.46×108CFU/mL;
The effective bacteria containing amount of Aspergillus flavus is 0.89 × 108-1.56×108CFU/mL;
The effective bacteria containing amount of bacillus licheniformis is 1.12 × 108-2.35×108CFU/mL;
The effective bacteria containing amount of Penicillium notatum is 1.07 × 108-2.43×108CFU/mL;
The mould effective bacteria containing amount of mine-laying silk branch is 0.78 × 108-1.28×108CFU/mL。
It further illustrates, the filter mud is beet-sugar factory sulfitation sugar mill filter mud;The filter mud carbon-nitrogen ratio is 30-40:
1。
A kind of processing method of alcohol waste as described in claim 1, cultivation matrix B are applied to the rich and honour dish of cultivation.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows:
The application in biomass to alcohol conversion process waste cassava residues and useless mash carry out reasonably handle after, difference can reduce wood
COD and BOD5 contents in potato waste residue and useless mash, and the cyanide content in waste cassava residues of degrading, and can be maintained at and meet state
The cultivation matrix obtained after fermentation process is carried out in the range of family's regulation with other raw materials, and cultivation matrix is exclusively for my ground
The rich and honour dish configuration just abounded with so that, in water retention property, the physicochemical properties such as organic composition content adapt to growth for it, improve rich
The quality of your dish.
【Specific implementation mode】
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to the specific reality of the present invention
The mode of applying is described in detail.Many details are elaborated in the following description in order to fully understand the present invention.But
The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hair
Similar improvement is done in the case of bright intension, therefore the present invention is not limited to the specific embodiments disclosed below.
Strain is all made of activated from preservation state by corresponding culture medium after use, wherein
The culture medium of the aspergillus oryzae is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 2.5g/L,
Banana puree 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000m L, pH to 6.8;
The culture medium of the candida valida is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone
1.0g/L, tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 7.2;
The culture medium of the Aspergillus flavus is made of following raw material:Apple polysaccharide 10.0g/L, agar 2.0g/L, potassium chloride
2.0g/L, epsom salt 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000m L, pH to 6.2;
The culture medium of the bacillus licheniformis is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone
1.0g/L, sodium chloride 2.0g/L, epsom salt 3.0g/L, ferrous sulfate 0.02g/L, deionized water 1000m L, pH to 7.0;
The culture medium of the Penicillium notatum is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.5g/L, chlorine
Change sodium 2.0g/L, corn steep liquor 3.5g/L, deionized water 1000m L, pH to 7.0;
The mould culture medium of the mine-laying silk branch is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.0g/
L, potassium chloride 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000m L, pH to 6.5.
Being understood that by the condition of adjusting activation culture and the time of activation can be obtained by the bacterium after above-mentioned activation
The bacterium of different effective bacteria containing amounts.
Embodiment 1:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 20 hours, obtains processing slurry by body;
The weight ratio of the waste cassava residues, white oil and zeolite is 20:2:1;
The addition that the mixing adjusts liquid is the 10% of jelly total weight;
The mixed microorganism is 1.03 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzaes, effective bacteria containing amount are 1.11
×108
CFU/mL candida validas, effective bacteria containing amount are 0.89 × 108CFU/mL Aspergillus flavus, effective bacteria containing amount are
1.12×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.07 × 108CFU/mL Penicillium notatums are 1 according to volume ratio:
1:1:2:2 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 40 hours afterwards, obtains handling useless mash;
It is 50 that the useless mash, mixing, which adjust liquid and mixed bacteria liquid volume ratio,:3:5;
The mixed bacteria liquid is 0.89 × 10 by effective bacteria containing amount8CFU/mL Aspergillus flavus, effective bacteria containing amount be 1.12 ×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.07 × 108CFU/mL Penicillium notatums, effective bacteria containing amount be 0.78 ×
108CFU/m mine-laying silk branches it is mould according to volume ratio be 1:2:1:2 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
50%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution of constitutive material is prepared by following methods in mixing adjusting liquid, is as follows:
S1 is extracted:The distilled water for adding raw materials into 10 times of volumes extracts 20 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, is to take supernatant after 2000r/min centrifuges handle 20s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 2ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resins
Column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is 1 according to volume ratio:2, which are mixed and made into lye, is eluted, and collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to the rich and honour dish of cultivation.
Comparative example 1:Processing scheme is substantially same as Example 1, difference be in the first step waste cassava residues without white oil
With zeolite milled processed.
Comparative example 2:Processing scheme is substantially same as Example 1, and difference is that waste cassava residues are not added with white oil in the first step
It is ground.
Comparative example 3:Processing scheme is substantially same as Example 1, and difference is that waste cassava residues are not added with zeolite in the first step
It is ground.
Verification test one:
Waste cassava residues after producing alcohol as raw material using cassava in comparative example 1-3, embodiment 1 are subjected to cyanide content
Detection, former content is 4.21 ± 0.27mg/kg, after milled processed process in the first step in comparative example 1-3, embodiment 1,
The content containing cyanide in waste cassava residues is detected, Parallel testing three times is done in detection every time, is averaged;Detection
As a result such as following table:
Table 1
Group | Cyanide content mg/kg before processing | Cyanide content mg/kg after processing | Degradation rate % |
Embodiment 1 | 4.21±0.27 | 1.21±0.18 | 71.2±0.04 |
Comparative example 1 | 4.21±0.27 | 4.18±0.21 | 0.71±0.02 |
Comparative example 2 | 4.21±0.27 | 2.22±0.15 | 47.3±0.10 |
Comparative example 3 | 4.21±0.27 | 2.43±0.22 | 42.3±0.05 |
As seen from the above table, using the technical solution of the application, the content for the cyanide that can greatly degrade compares not
The method degradation rate variation of this technology processing of use is little, and is greatly improved after illustrating the technical solution compounding of the application,
Increase rate is at least up to 40%.This is by the applicant the study found that being ground by white oil and zeolite and waste cassava residues,
White oil can be crosslinking in process of lapping in the cyanide in waste cassava residues, so that cyanide in the environment of grinding generates heat
Three keys fracture, to degrade the content of cyanide in waste cassava residues;And the addition of zeolite promotes and increases fever in grinding,
The efficiency of fracture can be quickly improved, the degradation of cyanide is further promoted.
By the waste cassava residues after producing alcohol as raw material using cassava in comparative example 1-3, embodiment 1 carry out COD value, BOD5 contains
The detection of amount, COD original contents are 311.34 ± 21.21mg/kg, and BOD5 original contents are 98.25 ± 12.07mg/kg, using comparison
In the first step in example 1-3, embodiment 1 after milled processed process, COD value, the BOD5 contents in waste cassava residues are detected,
Parallel testing three times is done in detection every time, is averaged;The result of detection such as following table:
Table 2
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
The method degradation rate variation for this technology processing not used is little, and is carried significantly after illustrating the technical solution compounding of the application
It is high.This is by the applicant the study found that being ground by white oil and zeolite and waste cassava residues, and white oil is in process of lapping
It can be embedded in the structure of waste cassava residues, so that COD, BOD5 oxidation in waste cassava residues divide in the environment of grinding generates heat
Solution, this researcher also found can be crosslinked by process of lapping white oil with zeolite, is improved and is changed zeolite structured cavernous structure
More intensive formation honeycomb is intensive, greatly improves the ability of CO absorption D, BOD5 of zeolite;This is used by being also demonstrated in table
The compounding raw material and proportioning of application are than the excellent effect of single use;The degradation rate of COD of comparing at least improves 17.2%,
The degradation rate of BOD5 at least improves 16.3%.
Embodiment 2:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 30 hours, obtains processing slurry by body;
The weight ratio of the waste cassava residues, white oil and zeolite is 30:5:3;
The addition that the mixing adjusts liquid is the 20% of jelly total weight;
The mixed microorganism is 2.11 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzaes, effective bacteria containing amount are 2.46
×108CFU/mL candida validas, effective bacteria containing amount are 1.56 × 108CFU/mL Aspergillus flavus, effective bacteria containing amount are 2.35
×108CFU/mL bacillus licheniformis, effective bacteria containing amount are 2.43 × 108CFU/mL Penicillium notatums are 2 according to volume ratio:3:4:3:
4 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 50 hours afterwards, obtains handling useless mash;
It is 80 that the useless mash, mixing, which adjust liquid and mixed bacteria liquid volume ratio,:5:9;
The mixed bacteria liquid is 1.56 × 10 by effective bacteria containing amount8CFU/mL Aspergillus flavus, effective bacteria containing amount be 2.35 ×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 2.43 × 108CFU/mL Penicillium notatums, effective bacteria containing amount be 1.28 ×
108CFU/m mine-laying silk branches it is mould according to volume ratio be 3:3:3:4 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
55%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution of constitutive material is prepared by following methods in mixing adjusting liquid, is as follows:
S1 is extracted:The distilled water for adding raw materials into 20 times of volumes extracts 30 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, is to take supernatant after 2500r/min centrifuges handle 30s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resins
Column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is 1 according to volume ratio:3, which are mixed and made into lye, is eluted, and collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to the rich and honour dish of cultivation.
Contrast groups 1:Processing scheme is substantially same as Example 1, difference be in the first step waste cassava residues without mixing
Microbial fermentation processing.
Contrast groups 2:Processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of aspergillus oryzae.
Contrast groups 3:Processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of candida valida.
Contrast groups 4:Processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of Aspergillus flavus.
Contrast groups 5:Processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of bacillus licheniformis.
Contrast groups 6:Processing scheme is substantially same as Example 1, and difference is the micro- life of the mixing added in the first step
Object is free of Penicillium notatum.
Verification test two:
By the waste cassava residues after producing alcohol as raw material using cassava in contrast groups 1-6, embodiment 2 carry out COD value, BOD5 contains
The detection of amount, COD original contents are 311.34 ± 21.21mg/kg, and BOD5 original contents are 98.25 ± 12.07mg/kg, by grinding
COD originals content is 107.35 ± 16.34mg/kg after processing, BOD5 original contents are 34.05 ± 5.23mg/kg, using contrast groups
In the first step in 1-6, embodiment 2 after fermentation treatments, COD value, the BOD5 contents in waste cassava residues are detected, often
Parallel testing three times is done in secondary detection, is averaged;The result of detection such as following table:
Table 3
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) degradation rate variation for this technology processing not used is little, and illustrates the technical solution compounding of the application
After substantially increase degradation rate.Degradation rate after compounding as seen from the table improves at least one compared to the degradation rate of single effect
Half, have the function that synergy, the applicant's research is the discovery that because inherently containing some harmful bacterias in waste cassava residues,
Harmful bacteria exist in the environment of so that COD, BOD5 content in waste cassava residues slowly increase, by the prescription after compounding from
The destructions such as the growth carbon source of different bacterium, PH, oxygen amount make harmful bacteria stress stop growing or be destroyed, the prescription after compounding
In can greatly COD, BOD5 content increase and can absorption degradations always.
Embodiment 3:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 25 hours, obtains processing slurry by body;
The weight ratio of the waste cassava residues, white oil and zeolite is 22:3:2;
The addition that the mixing adjusts liquid is the 13% of jelly total weight;
The mixed microorganism is 1.57 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzaes, effective bacteria containing amount are 1.64
×108CFU/mL candida validas, effective bacteria containing amount are 0.99 × 108CFU/mL Aspergillus flavus, effective bacteria containing amount are 1.73
×108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.64 × 108CFU/mL Penicillium notatums are 1 according to volume ratio:2:2:3:
3 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 44 hours afterwards, obtains handling useless mash;
It is 60 that the useless mash, mixing, which adjust liquid and mixed bacteria liquid volume ratio,:4:7;
The mixed bacteria liquid is 1.02 × 10 by effective bacteria containing amount8CFU/mL Aspergillus flavus, effective bacteria containing amount be 1.57 ×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.81 × 108CFU/mL Penicillium notatums, effective bacteria containing amount be 0.89 ×
108CFU/m mine-laying silk branches it is mould according to volume ratio be 1:2:2:3 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
52%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B.
Wherein, the extracting solution of constitutive material is prepared by following methods in mixing adjusting liquid, is as follows:
S1 is extracted:The distilled water for adding raw materials into 13 times of volumes extracts 27 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, is to take supernatant after 2000r/min centrifuges handle 25s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resins
Column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is 1 according to volume ratio:2, which are mixed and made into lye, is eluted, and collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to the rich and honour dish of cultivation.
Control group 1:Processing scheme is substantially same as Example 3, difference be in the first step waste cassava residues without mixing
Adjust liquid fermentation process.
Control group 2:Processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of long stamen bothriospermum chinense extracting solution.
Control group 3:Processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of Chinese herbaceous peony shikonin extract.
Control group 4:Processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of micro- fruit grass extracting solution.
Control group 5:Processing scheme is substantially same as Example 3, and difference is that the mixing added in the first step is adjusted
Liquid is free of purple perilla extracting solution.
Verification test three:
By the waste cassava residues after producing alcohol as raw material using cassava in control group 1-5, embodiment 3 carry out COD value, BOD5 contains
The detection of amount, COD original contents are 312.36 ± 20.04mg/kg, and BOD5 original contents are 97.69 ± 20.31mg/kg, by grinding
COD originals content is 104.36 ± 13.21mg/kg after processing, BOD5 original contents are 31.23 ± 6.98mg/kg, using contrast groups
In the first step in 1-5, embodiment 3 after fermentation treatments, COD value, the BOD5 contents in waste cassava residues are detected, often
Parallel testing three times is done in secondary detection, is averaged;The result of detection such as following table:
Table 4
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) degradation rate for this technology processing not used is told somebody what one's real intentions are, and is illustrated big after the technical solution of the application compounds
Improve degradation rate greatly.Degradation rate after compounding as seen from the table improves at least half compared to the degradation rate of single effect, reaches
The effect of synergy is arrived, the applicant's research is the discovery that in the adjusting liquid after compounding the organic acid containing there are many, in containing for organic acid
Under the action of amount and degradation can be greatly improved after microbial action, the organic acid of the hobby of each microorganism is to differ
Sample, situation after compounding has a service life for the extended activity microorganism for greatly promoting microorganism, specific mechanism each
The deviation of the specific organic acid of microorganism hobby is to be studied.
Embodiment 4:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 28 hours, obtains processing slurry by body;
The weight ratio of the waste cassava residues, white oil and zeolite is 27:4:2;
The addition that the mixing adjusts liquid is the 14% of jelly total weight;
The mixed microorganism is 1.86 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzaes, effective bacteria containing amount are 1.89
×108CFU/mL candida validas, effective bacteria containing amount are 1.21 × 108CFU/mL Aspergillus flavus, effective bacteria containing amount are 1.94
×108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.52 × 108CFU/mL Penicillium notatums are 1 according to volume ratio:2:3:2:
2 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 46 hours afterwards, obtains handling useless mash;
It is 70 that the useless mash, mixing, which adjust liquid and mixed bacteria liquid volume ratio,:4:8;
The mixed bacteria liquid is 1.19 × 10 by effective bacteria containing amount8CFU/mL Aspergillus flavus, effective bacteria containing amount be 1.69 ×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 1.32 × 108CFU/mL Penicillium notatums, effective bacteria containing amount be 1.10 ×
108CFU/m mine-laying silk branches it is mould according to volume ratio be 1:3:2:4 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
54%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, the extracting solution of constitutive material is prepared by following methods in mixing adjusting liquid, is as follows:
S1 is extracted:The distilled water for adding raw materials into 16 times of volumes extracts 25 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, is to take supernatant after 2500r/min centrifuges handle 26s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 3ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resins
Column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is 1 according to volume ratio:3, which are mixed and made into lye, is eluted, and collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to the rich and honour dish of cultivation.
Control 1:Processing scheme is substantially same as Example 4, and difference is that useless mash processing is not added with mixing in second step
Adjust liquid fermentation process.
Control 2:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without long stamen bothriospermum chinense extracting solution.
Control 3:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Chinese herbaceous peony shikonin extract.
Control 4:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without micro- fruit grass extracting solution.
Control 5:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without purple perilla extracting solution.
Control 6:Processing scheme is substantially same as Example 4, and difference is that useless mash processing is not added with mixing in second step
Bacterium solution fermentation process.
Control 7:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Aspergillus flavus.
Control 8:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without bacillus licheniformis.
Control 9:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
Without Penicillium notatum.
Control 10:Processing scheme is substantially same as Example 4, and difference is that the mixing added in second step adjusts liquid
It is mould without mine-laying silk branch.
Verification test four:
The useless mash after producing alcohol as raw material using cassava in 1-10, embodiment 4 will be compareed and carry out COD value, BOD5 contents
Detection, COD original contents be 986.36 ± 65.34mg/kg, BOD5 original contents be 215.36 ± 28.64mg/kg, to using pair
According to 1-10, embodiment 4 technology after to give up mash in COD value, BOD5 contents be detected, every time detection do it is parallel three times
Detection, is averaged;The result of detection such as following table:
Table 4
As seen from the above table, using the technical solution of the application, can greatly degrade COD value, the content of BOD5, compare
Method (contrast groups 1) degradation rate for this technology processing not used is told somebody what one's real intentions are, and is illustrated big after the technical solution of the application compounds
Improve degradation rate greatly.Use the degradation rate of the technical solution of embodiment 4 better than comparing the degradation rate of 1-10 as seen from the table,
And wherein unmixed adjusting liquid and the degradation rate of unmixed bacterium solution are minimum, and the mixing combined adjusts liquid and mixed bacteria liquid is equal
It is more excellent than the degradation effect of single component.
Embodiment 5:
A kind of processing method of alcohol waste, is as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp
Mixing is then adjusted liquid and mixed microorganism is added, after mixing stewing process 28 hours, obtains processing slurry by body;
The weight ratio of the waste cassava residues, white oil and zeolite is 27:4:2;
The addition that the mixing adjusts liquid is the 17% of jelly total weight;
The mixed microorganism is 2.07 × 10 by effective bacteria containing amount8CFU/mL aspergillus oryzaes, effective bacteria containing amount are 2.15
×108CFU/mL candida validas, effective bacteria containing amount are 1.34 × 108CFU/mL Aspergillus flavus, effective bacteria containing amount are 2.09
×108CFU/mL bacillus licheniformis, effective bacteria containing amount are 2.11 × 108CFU/mL Penicillium notatums are 1 according to volume ratio:2:2:3:
2 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, stirring
Room temperature stands 48 hours afterwards, obtains handling useless mash;
It is 76 that the useless mash, mixing, which adjust liquid and mixed bacteria liquid volume ratio,:4:7;
The mixed bacteria liquid is 1.34 × 10 by effective bacteria containing amount8CFU/mL Aspergillus flavus, effective bacteria containing amount be 2.08 ×
108CFU/mL bacillus licheniformis, effective bacteria containing amount are 2.09 × 108CFU/mL Penicillium notatums, effective bacteria containing amount be 1.07 ×
108CFU/m mine-laying silk branches it is mould according to volume ratio be 2:2:1:3 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new
Cadmium yellow beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, primary at interval of turning in 10 days, and control moisture content is
53%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, the extracting solution of constitutive material is prepared by following methods in mixing adjusting liquid, is as follows:
S1 is extracted:The distilled water for adding raw materials into 15 times of volumes extracts 28 points controlled at soxhlet extraction at 50 DEG C
Clock takes extracting solution, is to take supernatant after 2500r/min centrifuges handle 28s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 2ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 2ml/s speed by clear liquid along the absorption for being mounted with D284 weak-base anion-exchange resins
Column removes efflux;
S4, using the adsorption column in distilled water flushing S3, reuse by mass concentration be 15% potassium hydroxide solution and
The sodium hydroxide solution that mass concentration is 25% is 1 according to volume ratio:3, which are mixed and made into lye, is eluted, and collects eluent, i.e.,
Extracting solution can be obtained.
The above-mentioned cultivation matrix B being prepared is applied to the rich and honour dish of cultivation.
Experiment proves five:
The cultivation matrix that embodiment 1-5 is prepared plants rich and honour dish, and planting patterns is using conventional plantation
Environment and sprinkling method;
General planting method plants rich and honour dish using common cultivation matrix;
Cultivation matrix and common cultivation matrix are subjected to composition detection and physicochemical property detection;After the harvesting of rich and honour dish, and
Nutritional ingredient in rich and honour dish is detected;Specific detection table is as follows:
5 cultivation matrix physical property of table and common cultivation matrix contrast table
Classification | Total porosity/% | Water-holding porosity/% |
Embodiment 1 | 85.31 | 56.34 |
Embodiment 2 | 86.05 | 57.56 |
Embodiment 3 | 84.36 | 55.67 |
Embodiment 4 | 85.34 | 54.98 |
Embodiment 5 | 84.37 | 55.68 |
Common cultivation matrix | 65.46 | 46.84 |
6 cultivation matrix chemical property of table and common cultivation matrix contrast table
By upper table 5,6 it is found that using the physicochemical property of the application treated cultivation matrix than common cultivation matrix
Physicochemical property is excellent.
7 soilless culture sweet potato leaves nutritional ingredient of table and common cultivation substrate culture wealth and rank dish contrast table unit:mg/100g
As seen from the above table, the cultivation matrix prepared using the application can improve rich and honour dish for cultivating rich and honour dish
The comparision contents of potassium element vitamin B, C and P elements are apparent, improve the quality of rich and honour dish.
Following table is respectively the proportioning of constituent and percentage meter in embodiment 1-5:
8 matrix of table forms the proportioning table according to percent by weight
Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 5 | Embodiment 5 | |
Filter mud | 15% | 20% | 19% | 18% | 17% |
Fresh soya bean shell | 30% | 15% | 18% | 21% | 19% |
Cow dung | 25% | 15% | 30% | 28% | 20% |
Boiler ash sediment | 10% | 15% | 12% | 13% | 14% |
Sugarcane discards molasses | 10% | 10% | 6% | 5% | 7% |
Handle slurry | 5% | 15% | 7% | 9% | 14% |
The useless mash of processing | 5% | 10% | 8% | 6% | 9% |
Mixing adjusts proportioning table of the liquid according to percent by weight in 9 first step of table and second step
Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
Long stamen bothriospermum chinense extracting solution | 31% | 35% | 30% | 32% | 25% |
Purple perilla extracting solution | 20% | 15% | 20% | 18% | 19% |
Chinese herbaceous peony shikonin extract | 22% | 20% | 30% | 25% | 29% |
Micro- fruit grass extracting solution | 27% | 30% | 20% | 25% | 27% |
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of processing method of alcohol waste, which is characterized in that be as follows:
The first step:White oil is added in waste cassava residues after producing alcohol as raw material using cassava and zeolite is ground pulp body,
Then mixing is adjusted into liquid and mixed microorganism is added, stewing process 20-30 hours after mixing, obtain processing slurry;
The weight ratio of the waste cassava residues, white oil and zeolite is 20-30:2-5:1-3;
The addition that the mixing adjusts liquid is the 10%-20% of jelly total weight;
The mixed microorganism is by aspergillus oryzae, candida valida, Aspergillus flavus, bacillus licheniformis, Penicillium notatum according to body
Product is than being 1-2:1-3:1-4:2-3:2-4 mixtures form;
Second step:Mixing is added in useless mash after producing alcohol as raw material using cassava and adjusts liquid and mixed bacteria liquid, after stirring often
Temperature stands 40-50 hours, obtains handling useless mash;
The useless mash, mixing adjust liquid and mixed bacteria liquid volume ratio is 50-80:3-5:5-9;
It according to volume ratio is 1-3 that the mixed bacteria liquid is mould by Aspergillus flavus, bacillus licheniformis, Penicillium notatum, mine-laying silk branch:2-3:
1-3:2-4 mixtures form;
Third walks:Mash is given up in the processing that processing slurry that the first step obtains, second step are obtained and sugarcane discards molasses, new cadmium yellow
Beans shell, filter mud, cow dung, boiler ash sediment are uniformly mixed, and build heap fermentation, and primary at interval of turning in 10 days, control moisture content is 50-
55%, fermentation stops fermentation after 30 days and obtains cultivation matrix A, and then the moisture content of processing cultivation matrix A to 45% can be obtained
Cultivation matrix B,
Wherein, raw material according to the proportioning of percent by weight by being mixed, processing slurry 5%-15%, the useless mash 5%- of processing
10%, sugarcane discards molasses 5%-10%, fresh soya bean shell 15%-30%, filter mud 15%-20%, cow dung 15%-30%, stove
Lime-ash 10%-15%;
In the first step and second step, the mixing adjusts liquid and is formed according to weight percent mixture by following raw material:25%-
35% long stamen bothriospermum chinense extracting solution, 20%-30% Chinese herbaceous peonies shikonin extract, the micro- fruit grass extracting solutions of 20%-30%, 15%-20%
Purple perilla extracting solution.
2. a kind of processing method of alcohol waste according to claim 1, it is characterised in that:The mixing is adjusted in liquid
The extracting solution of constitutive material is prepared by following methods, is as follows:
S1 is extracted:The distilled water for adding raw materials into 10-20 times of volume extracts 20-30 controlled at soxhlet extraction at 50 DEG C
Minute, extracting solution is taken, is to take supernatant after 2000-2500r/min centrifuges handle 20-30s by revolution;
S2 depigmentations:Supernatant is toppled over for the speed of 2-3ml/s along absorption column wall with flow velocity, collects outflow adsorption column
Clear liquid;Deacidite D101 absorption resins are mounted in the adsorption column;
S3, with flow velocity for 2-3ml/s speed by clear liquid along the adsorption column for being mounted with D284 weak-base anion-exchange resins,
Remove efflux;
S4, using the adsorption column in distilled water flushing S3, reuse lye and eluted, collect eluent, you can extracted
Liquid.
3. a kind of processing method of alcohol waste according to claim 2, it is characterised in that:The lye is dense by quality
The sodium hydroxide solution that the potassium hydroxide solution and mass concentration that degree is 15% are 25% is 1 according to volume ratio:2-3 mixing systems
At.
4. a kind of processing method of alcohol waste according to claim 1, it is characterised in that:Strain is all made of from preservation
State uses after being activated by corresponding culture medium, wherein
The culture medium of the aspergillus oryzae is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 2.5g/L, banana
Mud 3.0g/L, sodium chloride 3.0g/L, potassium dihydrogen phosphate 3.0g/L, deionized water 1000mL, pH to 6.8;
The culture medium of the candida valida is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.0g/L,
Tea-seed oil 8.0g/L, magnesium sulfate 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000mL, pH to 7.2;
The culture medium of the Aspergillus flavus is made of following raw material:Apple polysaccharide 10.0g/L, agar 2.0g/L, potassium chloride 2.0g/
L, epsom salt 3.0g/L, potassium dihydrogen phosphate 2.0g/L, deionized water 1000mL, pH to 6.2;
The culture medium of the bacillus licheniformis is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.0g/L,
Sodium chloride 2.0g/L, epsom salt 3.0g/L, ferrous sulfate 0.02g/L, deionized water 1000mL, pH to 7.0;
The culture medium of the Penicillium notatum is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.5g/L, sodium chloride
2.0g/L, corn steep liquor 3.5g/L, deionized water 1000mL, pH to 7.0;
The mould culture medium of the mine-laying silk branch is made of following raw material:Apple polysaccharide 10.0g/L, soy peptone 1.0g/L, chlorine
Change potassium 1.5g/L, white vitriol 0.05g/L, potassium dihydrogen phosphate 1.5g/L, deionized water 1000mL, pH to 6.5.
5. a kind of processing method of alcohol waste according to claim 1, it is characterised in that:The aspergillus oryzae is effective
Bacteria containing amount is 1.03 × 108-2.11×108CFU/mL;
The effective bacteria containing amount of candida valida is 1.11 × 108-2.46×108CFU/mL;
The effective bacteria containing amount of Aspergillus flavus is 0.89 × 108-1.56×108CFU/mL;
The effective bacteria containing amount of bacillus licheniformis is 1.12 × 108-2.35×108CFU/mL;
The effective bacteria containing amount of Penicillium notatum is 1.07 × 108-2.43×108CFU/mL;
The mould effective bacteria containing amount of mine-laying silk branch is 0.78 × 108-1.28×108CFU/mL。
6. a kind of processing method of alcohol waste according to claim 1, it is characterised in that:The filter mud is beet sugar
Factory's sulfitation sugar mill filter mud;The filter mud carbon-nitrogen ratio is 30-40:1.
7. a kind of processing method of alcohol waste according to claim 1, it is characterised in that:Cultivation matrix B is applied to
The rich and honour dish of cultivation.
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