CN108497261A - A method of removing aflatoxin in biological raw material using strain fermentation - Google Patents
A method of removing aflatoxin in biological raw material using strain fermentation Download PDFInfo
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Abstract
The application belongs to technical field of biological fermentation, and in particular to a method of it is fermented using specific bacterial strain and removes aflatoxin in biological raw material.The specific bacterial strain is Phanerochaete chrysosporium and/or Coriolus Versicolor, and the biological raw material is using carbon as the biomass waste material of principal component and/or using nitrogen as the biomass waste material of principal component；It specifically includes：Biological raw material pretreatment, strain fermentation prepare zymotic fluid, scale fermentation processing or Rapid Fermentation aflatoxin degradation.In general, fermentation process provided herein, operation process are more convenient, aflatoxin degradation effect is more apparent, and processing cost is relatively low, can preferably food security, health lay the foundation, and has preferable practical value and promotes and applies meaning.
Description
Technical field
The application belongs to technical field of biological fermentation, and in particular to a kind of to be removed in biological raw material using specific bacterial strain fermentation
The method of aflatoxin.
Background technology
Aflatoxin (Aflatoxins, AFT) mainly by aspergillus flavus (Aspergillus flavus) and parasitism song
Mould (Aspergillus parasiticus) etc. it is a variety of it is mycetogenetic with extremely strong carcinogenic, mutagenesis and teratogenesis one
Class hypertoxicity metabolite.Aflatoxin is colourless, odorless, tasteless, containing there are one bifuran and one in molecular structure
Cumarin (cumarin), the former be basic toxin structure, the latter with it is carcinogenic related.The aflatoxin isolated reaches
18 kinds, to thermostabilization, high temperature resistant aflatoxin B1 toxin（AFB1）It is just decomposed at 268~269 DEG C；Highly basic and 5% time is added
Sodium chlorate can destroy completely；But stablizes in acid condition, slightly decomposed in the strongly acidic solution of pH value 1~3.Aspergillus flavus poison
Element dissolves in a variety of organic solvents such as chloroform, acetone, methanol, ethyl alcohol, but does not dissolve in hexane, petroleum ether, water and ether.
Due to storing improper or other reasons, some food or feedstuff（Such as peanut, corn and soybean, wheat, peanut
Cake（The dregs of rice）, dregs of beans, cotton dregs, rapeseed dregs etc.）Can often be infected by aspergillus flavus and aspergillus parasiticus so that in raw material containing compared with
The aflatoxin of high-content, aflatoxin content even can be up to 50 ~ 80 μ g/kg.Studies have shown that poultry is to yellow bent
Mould toxin is most sensitive, followed by piglet and sow, and cub is higher than adult domestic animal to the susceptibility of aflatoxin.In animal
In production, once daily ration（Such as corn, dregs of beans）It is aflatoxin-contaminated, gently then cause the reduction of animal body weight gains, feed to turn
Rate declines, heavy then induced tumor and cancer, or even dead, to cause serious economic loss.Achievement in research also table simultaneously
Bright, aflatoxin and canceration of hepatic cell (Liver Cell Cancer, LCC) being proportionate property, edible for a long time in food
The main reason for food of the aflatoxin containing low concentration is considered as leading to liver cancer gastric cancer, the diseases such as intestinal cancer.And by aspergillus flavus
The raw material of endotoxin contamination enters Feed Manufacturing or Edible Fungi field, eventually leads to that aflatoxin enters feed or food follows
Ring is to influence food security, an important channel for endangering human health and reason.
Since aflatoxin contamination range is relatively wide and toxicity is stronger, various countries are proposed the highest of aflatoxin
Limit standard, the content that China defines AFB1 is 20 μ g/kg, and European Union provides the highest of AFT in agricultural product (B1+B2+G1+G2)
Limitation is 4 μ g/kg, and the U.S. provides that the highest limitation of AFT in agricultural product (B1+B2+G1+G2) is 15 μ g/kg（Liu Yinkun, 1990；
Moss, 2002）.Therefore it is feed or fertilizer life to prevent from going mouldy and removing the aflatoxin in going mouldy feed or fertilizer material
Necessary link in production, and one of the basic measures that ensure food safety.
Currently on the market mainly there is the method for aflatoxin in common degrade feed or raw-food material：1, bioanalysis,
That is the directly degradation and absorption etc. of the microorganisms such as bacterium, actinomyces and saccharomycete；2, Physical, i.e. ultraviolet light technology, 268
DEG C or more high temperature, adsorbent absorption detoxification etc.；3, chemical method applies alkali process, oxidation processes etc..In general, these sides
Although method can remove the aflatoxin in raw material to a certain extent, all there are problems that, such as of high cost, secondary dirt
Dye, ineffective etc., therefore still need to the method new to the removal Research on Problems of aflatoxin in biological raw material.
Invention content
The application is designed to provide a kind of method removing aflatoxins in biological raw material using specific bacterial strain fermentation, from
And it is that the safe and reliable of the biological raw materials such as raw-food material or feedstuff lays the foundation.
Details are as follows for the technical solution that the application is taken.
A method of it is fermented using specific bacterial strain and removes aflatoxins in biological raw material, the specific bacterial strain is yellow archespore
The flat lead fungi of hair (Phanerochete chrysosporium) and/or Coriolus Versicolor (Coridus versicolor), the life
Raw material is using carbon as the biomass waste material of principal component（Maize straw, corncob, wheat bran, peanut vine etc.）And/or with nitrogen
For the biomass waste material of principal component（Peanut cake（The dregs of rice）, dregs of beans, cotton dregs, rapeseed dregs etc.）；It is as follows：
（One）Biological raw material pre-processes
（1.1）First, by the appropriate comminution pretreatment of biological raw material, for different types of biological raw material, specific pretreatment mode
It can refer to as follows：Maize straw, peanut vine stalk are cut into 0.5 ~ 3 centimetre to use again, after maize cob meal is broken to 5 ~ 20 mesh
It reuses, and is directed to peanut cake（The dregs of rice）, dregs of beans, cotton dregs, rapeseed dregs, can be used directly after cleanings, the dedusting such as peanut shell；
（1.2）Secondly, carbon-nitrogen ratio is adjusted（Also it can not adjust, directly carry out subsequent fermentation process）, adjust biology after pretreatment
Raw material carbon-nitrogen ratio is about C:N=1:0.1~0.5；It, specifically can be by being mixed into nitrogen manure when adjusting（Such as urea, ammonium bicarbonate）Deng
Form is adjusted, and also can refer to such as under type：
By the biomass waste material that the pending carbon by aflatoxin contamination is principal component（Maize straw, corncob, bran
Skin, peanut vine etc.）, with not by the nitrogen of aflatoxin contamination be principal component biomass waste material（Peanut cake（The dregs of rice）, beans
The dregs of rice, cotton dregs, rapeseed dregs etc.）In mass ratio 1:0.1 ~ 0.5 ratio is mixed；
Alternatively, by the biomass waste material that the pending nitrogen by aflatoxin contamination is principal component（Peanut cake（The dregs of rice）, beans
The dregs of rice, cotton dregs, rapeseed dregs etc.）, with not by the carbon of aflatoxin contamination be principal component biomass waste material（Maize straw,
Corncob, wheat bran, peanut vine etc.）In mass ratio 0.1 ~ 0.5:1 ratio is mixed；
（1.3）Finally, add water after being adjusted to carbon-nitrogen ratio in biological raw material, stir evenly, adjustment material water ratio is 35 ~ 65%（Matter
Amount ratio）As waiting for that fermentation product material is spare；
（Two）Strain fermentation prepares zymotic fluid
By Phanerochaete chrysosporium (Phanerochete chrysosporium) and Coriolus Versicolor (Coridus versicolor) difference fermentation process；
When fermented and cultured Phanerochaete chrysosporium, cultivation temperature is 37~39 DEG C, the Pseudomonas aleurioconidium state, specific culture side
Formula can refer to as follows：After tablet expands culture, in fluid nutrient medium, by 6 rings of inoculation per 100mL（Oese）Yellow archespore Mao Pingge
Bacterium spore meter cultivates 8 ~ 10d（Such as 9d）Left and right）；
It is as follows that culture medium can refer to design：
Phanerochaete chrysosporium culture medium：Glucose 10g/L, acetic acid-acetate buffer of ammonium tartrate 2mmol/L, pH 4.5
Liquid 10mmol/L, KH2PO42g/L, MgSO4·7H2O 0.5g/L, CaCl2·2H2O 0.1g/L, VB11mg/L, Li Lu alcohol
3mmol/L, micro-mixed liquor 7mL/L, 1 g/L of Tween 80；
The micro-mixed liquor composition is as follows：
Amion acetic acid 7.8 × 10-3Mol/L, MgSO4·7H2O 1.2×10-2Mol/L,
MnSO4·H2O 2.9×10-3Mol/L, NaCl 1.7 × 10-2Mol/L,
FeSO4·7H2O 3.59×10-4Mol/L, CoCl2 7.75×10-4Mol/L,
CaCl2·2H2O 9.0×10-4Mol/L, ZnSO4·7H2O 3.48×10-4Mol/L,
CuSO4·5H2O 4×10-5Mol/L, KAl (SO4)2·12H2O 2.1×10-5Mol/L,
HBO3 1.6×10-4Mol/L, NaMnO4 4.1×10-5mol/L；
When fermented and cultured Coriolus Versicolor, cultivation temperature be 28~30 DEG C, the Pseudomonas filamentous fungi, specific training method can refer to as
Under：After tablet expands culture, in fluid nutrient medium, 6 Coriolus Versicolor plugs, 8 ~ 10d of 150rpm shaken cultivations are met per 100mL
（Such as 9d）Left and right；
It is as follows that culture medium can refer to design：
Coriolus Versicolor culture medium：Glucose 2g/L, the acetic acid-sodium acetate buffer solution of ammonium tartrate 12mmol/L, pH4.5
10mmol/L, KH2PO4 1.47×10-2Mol/L, MgSO4·7H2O 2.03×10-3Mol/L, CaCl2·2H2O 6.8×10- 4Mol/L, VB1 2.97×10-6Mol/L, micro-mixed liquor 7mL/L, Tween 80 1g/L；
The micro-mixed liquor composition is same as above；
（Three）Fermentative degradation aflatoxin
Utilize step（Two）In zymotic fluid to step（One）Wait for fermentation product material carry out scale fermentation processing or quickly hair
Ferment degradation treatment, specifically：
Scale fermentation processing：By step（Two）Middle zymotic fluid, by 5 ~ 20% total inoculum concentration（Mass ratio）（That is, being inoculated with a kind of fermentation
When culture solution or two kinds of fermentation cultures, total inoculum concentration is 5 ~ 20%）, it is inoculated into step（One）Wait for fermentation product material, mix
After closing uniformly, natural packing fermented and cultured 8 ~ 15 days（When using Phanerochaete chrysosporium strain fermentation, fermentation temperature is controlled 30
~ 40 DEG C or so；When fermenting using Coriolus Versicolor, fermentation temperature is controlled at 28 ~ 32 DEG C or so；Two kinds of bacterial strains it is common in application,
Fermentation temperature is controlled at 30 ~ 35 DEG C, is preferably controlled in 32 DEG C or so；No matter using which kind of strain fermentation, ambient humidity controls
Under 70 ~ 85% relative humidities）, you can the aflatoxin in effective degradation biological raw material；
Rapid Fermentation processing：By step（Two）Middle zymotic fluid presses 1mL with biological raw material:0.1 ~ 1.5g ratios are mixed, 40
DEG C ~ 55 DEG C, 50 ~ 80h of fermentation process under the conditions of pH=4.0 ~ 5.0；Then it filters or centrifuges, after removing excessive moisture, institute's cutting
Material is harmless biological raw material after aflatoxin degradation.
Specific bacterial strain employed in the application be Phanerochaete chrysosporium (Phanerochete chrysosporium) and/
Or Coriolus Versicolor (Coridus versicolor), it is white-rot fungi.Preliminary studies have shown that containing in tunning
The enzyme of lignin can be decomposed by having, including lignin peroxidase, manganese-dependent peroxidase and a small amount of laccase, and be utilized
These enzymes can be in efficient degradation, removal biological raw material aflatoxin, the final innoxious place for realizing pollution raw material
Reason.On the other hand, certain cellulase, zytase etc. are also will produce in the fermentation culture of these bacterial strains, it can be one
The macromolecular substances such as cellulose, hemicellulose, starch etc. in degradation culture material in degree are determined, to the raw material conducive to the later stage
Conversion and utilization.
In general, fermentation process provided herein, operation process is more convenient, aflatoxin degradation effect
Fruit is more apparent, and processing cost is relatively low, can preferably food security, health lay the foundation, and has preferable practical valence
Value and popularization and application meaning.
Specific implementation mode
Explanation is further explained to the application with reference to embodiment, before introducing specific embodiment, with regard to following realities
The briefly introduction of part Experiment material situation involved in example is applied to be described as follows.
Phanerochaete chrysosporium (Phanerochete chrysosporium), Coriolus Versicolor (Coridus versicolor), white-rot fungi is belonged to, bacterial strain uses therefor is by Shanghai edible mushroom institute of the Chinese Academy of Agricultural Sciences favour in following embodiments
It gives, but the implementation of the present invention is not relying on specific bacterial strain.
Embodiment 1
The present embodiment mainly prepares aflatoxin degradation Phanerochaete chrysosporium zymotic fluid and Coriolus Versicolor zymotic fluid, specifically
Process is briefly discussed below.
（1）Phanerochaete chrysosporium zymotic fluid
Phanerochaete chrysosporium fermentation culture medium：Glucose 10g/L, acetic acid-acetic acid of ammonium tartrate 2mmol/L, pH 4.5
Sodium buffer solution 10mmol/L, KH2PO42g/L, MgSO4·7H2O 0.5g/L, CaCl2·2H2O 0.1g/L, VB11mg/L, multitude
Reed alcohol 3mmol/L, micro-mixed liquor 7mL/L, 1 g/L of Tween 80；
The micro-mixed liquor composition is as follows：
Amion acetic acid 7.8 × 10-3Mol/L, MgSO4·7H2O 1.2×10-2Mol/L,
MnSO4·H2O 2.9×10-3Mol/L, NaCl 1.7 × 10-2Mol/L,
FeSO4·7H2O 3.59×10-4Mol/L, CoCl2 7.75×10-4Mol/L,
CaCl2·2H2O 9.0×10-4Mol/L, ZnSO4·7H2O 3.48×10-4Mol/L,
CuSO4·5H2O 4×10-5Mol/L, KAl (SO4)2·12H2O 2.1×10-5Mol/L,
HBO3 1.6×10-4Mol/L, NaMnO4 4.1×10-5mol/L；
When fermented and cultured, 6 rings are connect by every 100mL（Oese）Phanerochaete chrysosporium spore, 37~39 DEG C, static gas wave refrigerator 9d,
Obtain Phanerochaete chrysosporium bacteria suspension（Zymotic fluid）It is spare.
（2）Coriolus Versicolor zymotic fluid
Coriolus Versicolor culture medium：Glucose 2g/L, the acetic acid-sodium acetate buffer solution of ammonium tartrate 12mmol/L, pH4.5
10mmol/L, KH2PO4 1.47×10-2Mol/L, MgSO4·7H2O 2.03×10-3Mol/L, CaCl2·2H2O 6.8×10- 4Mol/L, VB1 2.97×10-6Mol/L, micro-mixed liquor 7mL/L, Tween 80 1g/L；
The micro-mixed liquor composition is same as above；
When fermented and cultured, 6 Coriolus Versicolor bacterium plugs are connect by every 100mL, 28~30 DEG C, 150rpm shaken cultivation 7d are obtained variegated
Rainbow conk bacteria suspension（Zymotic fluid）It is spare.
Embodiment 2
The biological raw material that the present embodiment is directed to is the corncob for polluting aflatoxin, and when specific fermentative degradation is handled, process is such as
Under：
（One）Biological raw material pre-processes, and adjusts carbon-nitrogen ratio
（1.1）First, by biological raw material corncob（Aflatoxin contamination）It is spare to be crushed to 10 mesh or so；
（1.2）Secondly, it by the pending corncob by aflatoxin contamination, is pressed with the dregs of beans of no pollution aflatoxin
1:0.2（Mass ratio）It is uniformly mixed；
（1.3）Finally, add water after being adjusted to carbon-nitrogen ratio in biological raw material, stir evenly, adjustment material water ratio is 55%（Quality
Than）Left and right, as waiting for that fermentation product material is spare.
（Two）Fermentative degradation aflatoxin
Scale fermentation processing：By Phanerochaete chrysosporium zymotic fluid in embodiment 1, by 10% inoculum concentration（Mass ratio）, it is inoculated into step
Suddenly（One）Wait for fermentation product material, after mixing, natural packing fermented and cultured 15 days（Temperature control is at 35 ~ 40 DEG C, humidity
Control the relative humidity 70 ~ 85%）Left and right.
After fermentation, aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content
16.7 μ g/Kg are reduced to by 69.8 μ g/Kg before fermenting, reducing effect is apparent.
Embodiment 3
The biological raw material that the present embodiment is directed to is the dregs of beans for polluting aflatoxin, and when specific fermentative degradation is handled, process is big
For body with embodiment 2, only adjustment member parameter is as follows：
Step（One）In, to pollute the dregs of beans of aflatoxin and without polluting the wheat bran of aflatoxin by 0.2:1（Quality
Than）And it is 50% to adjust moisture content（Mass ratio）Mixed material, as waiting for fermentation product material；
Step（Two）In, using Coriolus Versicolor zymotic fluid in embodiment 1, press 13% inoculum concentration（Mass ratio）, natural packing culture 15
Its fermentation process.
After fermentation, aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content
10.6 μ g/Kg are reduced to by 60.7 μ g/Kg before fermenting, reducing effect is apparent.
Embodiment 4
The biological raw material that the present embodiment is directed to is the maize straw for polluting aflatoxin, when specific fermentative degradation is handled, mistake
For Cheng great Ti with embodiment 2, only adjustment member parameter is as follows：
Step（One）In, after the maize straw for polluting aflatoxin is cut into 0.5 ~ 3 centimetre, with no pollution aflatoxin
Peanut meal press 1:0.2（Mass ratio）After mixing, material water ratio is adjusted 55%（Mass ratio）Left and right, as waiting for fermentation process
Material；
Step（Two）In, while using Phanerochaete chrysosporium zymotic fluid and Coriolus Versicolor zymotic fluid in embodiment 1, it is total by 10%
Inoculum concentration（Phanerochaete chrysosporium zymotic fluid inoculum concentration is 5%, and Coriolus Versicolor zymotic fluid inoculum concentration is 5%）, natural packing culture
15 days fermentation process.
After fermentation, aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content
15.7 μ g/Kg are reduced to by 79.7 μ g/Kg before fermenting, reducing effect is apparent.
Embodiment 5
The biological raw material that the present embodiment is directed to is the peanut shell for polluting aflatoxin, specific fermentation process different from embodiment 2
When, it is handled using Rapid Fermentation processing mode, specifically：
By the peanut shell of Phanerochaete chrysosporium zymotic fluid prepared by embodiment 1 and pollution aflatoxin, by 1mL：0.5g's
After mixing, 72h is acted under the conditions of 45 DEG C, pH=4.5 for ratio；Filtering removal excessive moisture is aflatoxin degradation
Harmless biological raw material afterwards.
Aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content by fermenting before
65.5 μ g/Kg be reduced to 18.8 μ g/Kg, reducing effect is apparent.
Embodiment 6
The biological raw material that the present embodiment is directed to is the soybean for polluting aflatoxin, similar to Example 5, same using quickly hair
Ferment processing mode is handled, specifically：
By the soybean of Phanerochaete chrysosporium zymotic fluid prepared by embodiment 1 and pollution aflatoxin, by 1mL：The ratio of 1g,
After mixing, 72h is acted under the conditions of 45 DEG C, pH=4.8；Filtering removal excessive moisture is nothing after aflatoxin degradation
Evil biological raw material.
Aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content by fermenting before
72.2 μ g/Kg be reduced to 16.7 μ g/Kg, reducing effect is apparent.
Embodiment 7
The biological raw material that the present embodiment is directed to is the peanut meal for polluting aflatoxin, similar to Example 5, same using quick
Fermentation process mode is handled, specifically：
By the peanut meal of Phanerochaete chrysosporium zymotic fluid prepared by embodiment 1 and pollution aflatoxin, by 1mL：The ratio of 1g
Example, after mixing, 68h is acted under the conditions of 50 DEG C, pH=4.5；Filtering removal excessive moisture is after aflatoxin is degraded
Harmless biological raw material.
Aflatoxin content in biological raw material is measured, the results showed that, aflatoxin content by fermenting before
73.6 μ g/Kg be reduced to 15.1 μ g/Kg, reducing effect is apparent.
Based on the above embodiments as can be seen that when the biological raw material in face of a large amount of aflatoxin contaminations needs degradation,
Scale fermentation mode may be used to be handled, and it is less or when processing time is shorter when needing to handle biological raw material amount, it can
It is handled using Rapid Fermentation mode, but no matter which kind of mode, can obtain the effect of preferable aflatoxin degradation.
Claims (5)
1. a kind of method of aflatoxins in fermentation removal biological raw material using specific bacterial strain, which is characterized in that the specified germ
Strain is Phanerochaete chrysosporium and/or Coriolus Versicolor, the biological raw material be using carbon as the biomass waste material of principal component and/or
Using nitrogen as the biomass waste material of principal component；It is as follows：
（One）Biological raw material pre-processes
First, biological raw material is pre-processed；
Secondly, adjusting carbon-nitrogen ratio is C:N=1:0.1~0.5；
Finally, add water after being adjusted to carbon-nitrogen ratio in biological raw material, stir evenly, adjustment material water ratio is 35 ~ 65% as pending
It is spare that ferment handles material；
（Two）Strain fermentation prepares zymotic fluid
Phanerochaete chrysosporium and Coriolus Versicolor are distinguished into fermentation process；
When fermented and cultured Phanerochaete chrysosporium, cultivation temperature is 37~39 DEG C；
When fermented and cultured Coriolus Versicolor, cultivation temperature is 28~30 DEG C；
（Three）Fermentative degradation aflatoxin
Utilize step（Two）In zymotic fluid to step（One）Wait for fermentation product material carry out scale fermentation processing or quickly hair
Ferment degradation treatment, specifically：
Scale fermentation processing：By step（Two）Middle zymotic fluid is inoculated into step by 5 ~ 20% total inoculum concentration（One）Wait ferment
Handle material, after mixing, natural packing fermented and cultured 8 ~ 15 days, you can the aspergillus flavus poison in effective degradation biological raw material
Element；
Rapid Fermentation processing：By step（Two）Middle zymotic fluid presses 1mL with biological raw material:0.1 ~ 1.5g ratios are mixed, 40
DEG C ~ 55 DEG C, 50 ~ 80h of fermentation process under the conditions of pH=4.0 ~ 5.0；Harvested material is harmless biology after aflatoxin degradation
Raw material.
2. utilizing the method for aflatoxins in specific bacterial strain fermentation removal biological raw material as described in claim 1, which is characterized in that
The biological raw material is to be by the biomass waste material of principal component of carbon：It is a kind of in maize straw, corncob, wheat bran, peanut vine
Or several arbitrary proportion mixtures；
The nitrogen is that the biomass waste material of principal component is：One or more of arbitrary ratios in peanut cake, dregs of beans, cotton dregs, rapeseed dregs
Example mixture.
3. utilizing the method for aflatoxins in specific bacterial strain fermentation removal biological raw material as claimed in claim 2, which is characterized in that
Step（One）In, the pretreatment mode is：Maize straw, peanut vine stalk are cut into 0.5 ~ 3 centimetre to use again, by corn
Core reuses after being crushed to 5 ~ 20 mesh, for peanut cake, dregs of beans, cotton dregs, rapeseed dregs, peanut shell, directly makes after cleaning, dedusting
With.
4. utilizing the method for aflatoxins in specific bacterial strain fermentation removal biological raw material as claimed in claim 2, which is characterized in that
Step（One）In, the specific regulative mode for adjusting carbon-nitrogen ratio is：It is adjusted by being mixed into nitrogen manure, or according to such as lower section
Formula is adjusted：
It is and dirty not by aflatoxin by the biomass waste material that the pending carbon by aflatoxin contamination is principal component
The nitrogen of dye is the biomass waste material in mass ratio 1 of principal component:0.1 ~ 0.5 ratio is mixed；
Alternatively, by the biomass waste material that the pending nitrogen by aflatoxin contamination is principal component, and not by aspergillus flavus
The carbon of endotoxin contamination is the biomass waste material in mass ratio 0.1 ~ 0.5 of principal component:1 ratio is mixed.
5. utilizing the method for aflatoxins in specific bacterial strain fermentation removal biological raw material as described in claim 1, which is characterized in that
Step（Two）In, when fermented and cultured, culture medium prescription design is as follows：
Phanerochaete chrysosporium culture medium：Glucose 10g/L, acetic acid-acetate buffer of ammonium tartrate 2mmol/L, pH 4.5
Liquid 10mmol/L, KH2PO42g/L, MgSO4·7H2O 0.5g/L, CaCl2·2H2O 0.1g/L, VB11mg/L, Li Lu alcohol
3mmol/L, micro-mixed liquor 7mL/L, 1 g/L of Tween 80；
Coriolus Versicolor culture medium：Glucose 2g/L, the acetic acid-sodium acetate buffer solution of ammonium tartrate 12mmol/L, pH4.5
10mmol/L, KH2PO4 1.47×10-2Mol/L, MgSO4·7H2O 2.03×10-3Mol/L, CaCl2·2H2O 6.8×10- 4Mol/L, VB1 2.97×10-6Mol/L, micro-mixed liquor 7mL/L, Tween 80 1g/L；
The micro-mixed liquor composition is as follows：
Amion acetic acid 7.8 × 10-3Mol/L, MgSO4·7H2O 1.2×10-2Mol/L,
MnSO4·H2O 2.9×10-3Mol/L, NaCl 1.7 × 10-2Mol/L,
FeSO4·7H2O 3.59×10-4Mol/L, CoCl2 7.75×10-4Mol/L,
CaCl2·2H2O 9.0×10-4Mol/L, ZnSO4·7H2O 3.48×10-4Mol/L,
CuSO4·5H2O 4×10-5Mol/L, KAl (SO4)2·12H2O 2.1×10-5Mol/L,
HBO3 1.6×10-4Mol/L, NaMnO4 4.1×10-5mol/L。
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