CN107164437A - Aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal - Google Patents
Aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal Download PDFInfo
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- CN107164437A CN107164437A CN201710504127.0A CN201710504127A CN107164437A CN 107164437 A CN107164437 A CN 107164437A CN 201710504127 A CN201710504127 A CN 201710504127A CN 107164437 A CN107164437 A CN 107164437A
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- peanut
- peanut meal
- protein peptide
- aflatoxin
- fermentation
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses aflatoxin degraded in a kind of peanut meal and the synchronous method for preparing high nutrition peanut protein peptide, the peanut protein peptide product of safety, high nutrition is made including going out the steps such as reason, microorganism segmentation inoculation fermentation, drying, crushing before peanut meal, the invention provides aflatoxin and the synchronous method for preparing high nutrition peanut protein peptide in a kind of highly effective and safe degraded peanut meal, can can aflatoxin degradation in solid fermentation process, the peanut protein peptide safety of preparation, nutrition simultaneously, available for food and health product；This method is simple to operate, and energy consumption is low, it is easy to industrialized production.
Description
Technical field
The present invention relates to aflatoxin degraded in a kind of peanut meal and the synchronous method for preparing high nutrition peanut protein peptide,
Belong to peanut food safety and field of nutrition.
Background technology
China is peanut production big country, and about 3,000,000 tons of peanut meal can be obtained after annual peanut oil expression.Contain in peanut meal
Abundant nutritional ingredient, such as peanut protein, flavonoids, carbohydrate.Wherein peanut protein with application wider soybean protein compared with,
Have the advantages that the factor containing ventosity and ANFs are less；Compared with vegetable seed, cottonseed protein, with contained toxicant
Less advantage.But peanut meal utilization rate is low, mainly as feed, cheap, about 2600 yuan/ton now, far below soybean
The price of the dregs of rice（3200 yuan/ton）.Check prince and wide variety of principal element are that the yellow aspergillus of its easy infection produces aspergillus flavus poison
Element.
Aflatoxin has strong toxicity and carcinogenicity, seriously endangers human and livestock health, only 0.294 mg/kg dosage is with regard to energy
Cause the acute poisoning of Sensitivity animal dead（Rawal, et al., 2010；Kensler et al., 2011）.Aspergillus flavus poison
Disposition matter is stable, and general processing method can not remove its toxicity.For many years, scientists are directed to utilizing physics, chemistry, biology
Aflatoxin is removed etc. a variety of methods.Traditional physics and the aflatoxin detoxicating method of chemistry include ozone, ammonification
Method, alkaline process, x ray irradiation x method, absorption method and Ultrafiltration-Diafiltration method etc., these methods all be present, such as cause food and
Nutritive loss in feed, influences organoleptic quality, equipment price costliness required for some methods etc.；Next some method
Reaction mechanism is reversible, and the toxicity of aflatoxin can be reappeared, and these all limit traditional physico-chemical method in actual life
Application in production.
Bioanalysis, is the method using metabolite aflatoxin degradations such as the enzymes of microorganism and its secretion, with effect
Rate is high, high specificity and the characteristics of do not polluted to food, feed and environment.It is biodegradable both at home and abroad to remove aflatoxin
Research be concentrated mainly on microbial strains and directly act on aflatoxin, and the enzyme that bacterial strain is produced makes preparation effect
In aflatoxin.And the Study on degradation for aflatoxin in complex matrices such as peanut meal is less.
Strain involved by biodegradation relates generally to armillariella tabescens, slime bacteria, the narrow food unit cell of thermophilic malt of the country at present
Orange Flavobacterium and Rhodococcus erythropolis of bacterium and foreign study etc., but exist bacterial strain be difficult in peanut meal growth and breeding,
Degradation rate is low, some bacterial strains can not be applied in food production, the problems such as having certain potential safety hazard.And be all research single strain
To the degradation of aflatoxin, do not report that many bacterium combine the degradation to aflatoxin.
Contain substantial amounts of albumen and fiber in peanut meal simultaneously, the preparations of protein peptides in peanut meal is reported for work more but right
Aflatoxin in albumen there are no reporting for work for removal, cause there is aflatoxin in the protein peptides prepared, influence disappears
The health of the person of expense.It there are no at present and remove reporting for work for aflatoxin while protein peptides are prepared.
Reporting for work for peanut protein peptide is prepared for enzyme process more, patent 201310167006.3 discloses a kind of double neutral eggs
The method that white enzyme distribution enzymolysis peanut protein isolate prepares peanut peptide, the patent system is molten for protein isolate for the method for peanut peptide
Peanut protein peptide is made in solution, enzymolysis, centrifugation, spray drying.The patent adds substantial amounts of water and carries out enzyme when preparing protein peptides
It is spray-dried after solution, enzymolysis, this method undoubtedly adds energy consumption cost in process of production, adds the production of product
Cost.
The content of the invention
In order to which efficient, low energy utilizes the protein in peanut meal, the aflatoxin in safe disposal peanut meal, flower is improved
The nutrition of the raw dregs of rice and value, eliminate the harm that aflatoxin is utilized to peanut meal, the invention provides a kind of peanut meal
Middle aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide, utilize rainbow conk, bacillus subtilis, aspergillus niger
The combined solid fermentation peanut meal of three kinds of strains produces aflatoxin degradation enzyme, proteolytic enzyme and cellulase etc.,
Distilled water is added into culture medium by timing during enzymatic production, it is ensured that the biology enzyme that microbial fermentation is produced can be solid
Aflatoxin in degraded peanut meal in the state of body fermentation, while being digested to the albumen and fiber in peanut meal, in drop
While solving aflatoxin, the peanut protein peptide of high nutrition is prepared.
To reach above-mentioned purpose, the technical solution adopted in the present invention has the following steps：
1st, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal, its feature include
Following steps：
（1）Feedstock treating：Peanut meal is crushed, 40 mesh sieves are crossed；A certain amount of distilled water is added into peanut meal powder, is sterilized；
（2）Microorganism is segmented inoculation fermentation：The rainbow conk strain after a certain amount of activation, 25-40 DEG C are inoculated with into the peanut meal of sterilizing
Solid fermentation 5-8 d, then the bacillus subtilis activated, 30-35 DEG C of solid fermentation 3-5 are inoculated with into the peanut meal of fermentation
D, then the aspergillus niger activated, 28-35 DEG C of solid fermentation 3-5 d are inoculated with into peanut meal；During the fermentation, every other day to
The distilled water of certain volume is added in fermentation medium；
（3）Dry, crush：Peanut meal 40-50 after fermentation is dried DEG C, ultramicro grinding, both nontoxic, high nutrition peanut protein
Peptide.
2nd, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（1）Described peanut meal powder and the adding proportion (g of distilled water:Ml it is) 1:2-1:3.
3rd, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The activation method of described rainbow conk is：By 6-8 strain plugs（7mm diameters）Rainbow conk bacterial strain be inoculated in
During 10 g diameters are about 5 mm Roots of Peanut powder, rainbow conk activated spawn is made in 25-30 DEG C of activation culture 5-7d.
4th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The inoculum concentration of described rainbow conk is：The mass ratio of peanut meal and activated spawn（g:g）For 20:1-
20:2。
5th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The activation method of described bacillus subtilis is：Bacillus subtilis is inoculated in peanut meal water
In solution, Bacillus subtilis strain liquid is made in 25-30 DEG C of activation culture 2-3 d.
6th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The inoculum concentration of described bacillus subtilis is：The mass volume ratio of peanut meal and activated spawn
（g:ml）For 20:1-20:2.
7th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The activation method of described aspergillus niger is：The Aspergillus niger strain of 2-3 strain rings is inoculated in 10 g
During diameter is about 5mm Roots of Peanut powder, 25-30 DEG C of activation culture 3-5d, get processed aspergillus activated spawn.
8th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The inoculum concentration of described aspergillus niger is：The mass ratio of peanut meal and activated spawn（g:g）For 20:1-
20:2。
9th, preferably, aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal,
Characterized in that, step（2）The addition of distilled water is in described fermentation process：The mass ratio of peanut meal and distilled water is：
1:1-2:3。
Beneficial effects of the present invention are as follows：
（1）It is cost-effective
Distilled water is added in the use of the invention during solid fermentation, enables the biology enzyme that fermentation is produced in solid
Enzyme digestion reaction is carried out in fermentation process, eliminates and is adding the process of substantial amounts of water progress enzyme digestion reaction after fermentation, without carrying out
Spray drying, has saved a large amount of energy consumption costs, has reduced the production cycle.
（2）Comprehensive aflatoxin degradation B1、B2、G1、G2
The present invention cooperates with stepwise fermentation peanut meal can be with efficient degradation aspergillus flavus poison by rainbow conk, bacillus subtilis and aspergillus niger
Element.Rainbow conk can produce the enzyme preparation of aflatoxin degradation, while bacillus subtilis and aspergillus niger are containing aspergillus flavus poison
The enzyme for producing aflatoxin degradation can also be induced in the peanut meal of element, three kinds of synergistic bacterium fermentations not only can efficiently drop
Solve aflatoxin B1, can be with aflatoxin degradation B2And aflatoxin G1And G2, aspergillus flavus can be improved by serving
Toxin B1 degradation rate, can play the unexpected effect of other aflatoxin etc. of degrading comprehensively again.
（3）Degraded cyclopiazonic acid
Cyclopiazonic acid (Cyclopiazonic acid, CPA) is that a kind of indoles spreads out thing (Indole-derived ergot
Alkaloids), it is a kind of mycotoxin for producing of several fungal secondaries metabolism of aspergillus and Penicillium, toxicologic study table
Bright, it is a kind of neurotoxin, can inducing neural system disorders.Cyclopiazonic acid and aflatoxin often exist jointly.This
Invention cooperates with stepwise fermentation peanut meal to produce the yellow song that can not only degrade by rainbow conk, bacillus subtilis and aspergillus niger
Mould toxin, can also degrade cyclopiazonic acid, it is ensured that the safety of peanut meal.
（4）Three kinds of bacterium collaboration stepwise fermentations contribute to the growth and breeding of three kinds of bacterium.
The battalion that peanut meal is provided fermentation strain rainbow conk, bacillus subtilis and aspergillus niger as unique source of nutrition
Support less, rainbow conk, bacillus subtilis and aspergillus niger can secret out of in same culture medium top fermentation and grow required battalion each other
Material is supported, promotes the growth of various bacterium, shortens fermentation time.
（5）Subsection enzymolysis is favorably improved the degradation rate and peanut protein peptide of aflatoxin after three kinds of cooperative fermentations
Yield.
Three kinds of bacterium can produce the protease of different cultivars, be conducive to comprehensive enzymolysis of protein in peanut meal, not only may be used
To improve the yield of peanut protein peptide, aflatoxin and peanut protein can be separated, be conducive to the dissolution of aflatoxin
And degraded, play a part of improving the degradation rate of aflatoxin；The cellulase that three kinds of bacterium produce simultaneously can digest peanut
Cellulose in the dregs of rice, is conducive to the dissolution of peanut protein and aflatoxin, improves the degradation rate and peanut egg of aflatoxin
The yield of white peptide.
（6）After rainbow conk is activated on Roots of Peanut culture medium, vigor is improved.Shorten the fermentation time of peanut meal；Spend simultaneously
Contain various active material in taking root, not only acted as the effect of increase peanut meal nutrition, also act in fermentation process and suppress
The effect of varied bacteria growing.
（7）Bacillus subtilis is activated in the peanut meal aqueous solution, and producing bacillus subtilis life can be induced to digest
Peanut cake protein（It is denatured）Enzyme, play a part of improve peanut protein peptide yield.
（8）The peanut protein peptide of preparation does not contain harmful substance, and remains the nutritive value of peanut；While three kinds of bacterium
Cooperative fermentation also add the more healthcare functions of peanut protein peptide, such as enhancing immunity of organisms, anti-aging, enhancing study note
Recall ability, promote the work(such as nucleic acid, the synthesis of protein, liver protection, removing toxic substances and treatment hypertension and hyperlipemia, protection angiocarpy
Effect.
（9）Peanut protein peptide product has preferable antioxidation activity, such as remove DPPH free radicals, scavenging hydroxyl,
Remove ultra-oxygen anion free radical, iron reducing power, molybdenum reducing power, iron ion chelating ability, chelating copper ions power, suppression linoleic acid mistake
Oxidability, anti-peroxidation ability etc..Therefore, it can be used to delay body aging as a kind of functional food, in advance
The generation of anti-" three high " disease, it is also possible to prevent food because of fat with the food industry as a kind of natural
Fat is aoxidized and gone bad, and improves the quality stability in Food Shelf-life.In addition, peanut antioxidant peptide product also has preferable work(
Can property, such as emulsibility, emulsion stability, foaming characteristic, foam stability, dissolubility, water imbibition, oil absorption.In food plus
During work, it can assign food distinctive processing characteristics, finished product is had special functional character.
（10）Aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide use three in a kind of peanut meal
Cooperative fermentation is planted, subsection enzymolysis while aflatoxin degradation, prepares peanut protein peptide, reduces operating process, shortens
Process time, cost-effective, operating method is simple, it is easy to industrialized production.
Embodiment
Embodiment 1
Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/kg.10g peanut meals add
Enter 20ml water, sterilize, inoculation is through Roots of Peanut fermentation activation rainbow conk strain 1g, 25 DEG C of solid fermentation 8d, then to the peanut of fermentation
Bacillus subtilis strain 1ml, 30 DEG C of d of solid fermentation 3 that peanut meal activated in water solution is crossed are inoculated with the dregs of rice, then into peanut meal
Aspergillus niger 1g, 35 DEG C of d of solid fermentation 3 that inoculation Roots of Peanut was activated；Culture medium after fermentation adds 30ml distilled water, 33
DEG C concussion enzymolysis 2h after, 25 DEG C concussion enzymolysis 8h；100 DEG C of min of destroy the enzyme treatment 15 of enzymolysis liquid, be cooled to after room temperature with a high speed from
Scheming 10000rpm centrifuges 10 min；The supernatant that centrifugation is obtained is spray-dried, and pan feeding temperature is 50 DEG C, EAT
For 180 DEG C, intake is 22h, and peanut protein peptide is obtained after the completion of drying.Peanut protein peptide yield is 85.21%, peanut protein
Aflatoxin B in peptide1、B2、G1、G2Do not detected with cyclopiazonic acid.The antioxidation activity of the anti-oxidation peptide is as follows：Remove
The IC of DPPH free radicals50It is worth for 9.33mg/mL, the IC of scavenging hydroxyl50Be worth for 4.09mg/mL, remove superoxide anion from
By the IC of base50It is worth for 5.98mg/mL, the concentration of peanut antioxidant peptide is 6.37mg/ needed for when the light absorption value of iron reducing power is 0.5
ML, the concentration of peanut antioxidant peptide needed for when the light absorption value of molybdenum reducing power is 0.5 is 5.11mg/mL, the IC of iron ion chelating ability50
It is worth for 5.47mg/mL, the IC of chelating copper ions power50It is worth for 3.58mg/mL, the IC of suppression linoleic acid peroxidation ability50It is worth and is
The IC of 4.34mg/mL anti-lipid peroxidation abilities50It is worth for 4.95mg/mL.
Control experiment：Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/kg
.10g peanut meals add 20ml water, sterilizing, and inoculation is through Roots of Peanut fermentation activation rainbow conk strain 1g, 25 DEG C of solid fermentation 8d, 30
DEG C solid fermentation 3 d, 35 DEG C of d of solid fermentation 3；Culture medium after fermentation adds 30ml distilled water, 33 DEG C of concussion enzymolysis 2h
Afterwards, 25 DEG C of concussion enzymolysis 8h；100 DEG C of min of destroy the enzyme treatment 15 of enzymolysis liquid, are cooled to after room temperature and use supercentrifuge 10000rpm
Centrifuge 10 min；The supernatant that centrifugation is obtained is spray-dried, and pan feeding temperature is 50 DEG C, and EAT is 180 DEG C, air intake
Measure as 22h, peanut protein peptide is obtained after the completion of drying.Peanut protein peptide yield is aflatoxin in 5.21%, peanut protein peptide
B1、B2、G1、G2It is 42 μ g/kg for 51 μ g/kg and cyclopiazonic acid content.The anti-oxidation peptide is without antioxidation activity.
Embodiment 2
Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/kg.10g peanut meals add
Enter 20ml water, sterilize, inoculation is through Roots of Peanut fermentation activation rainbow conk strain 0.7g, 28 DEG C of solid fermentation 6d, then to the flower of fermentation
Inoculation peanut meal activated in water solution is crossed in the raw dregs of rice Bacillus subtilis strain 0.7ml, 32 DEG C of d of solid fermentation 4, then to peanut
Aspergillus niger 0.7g, 32 DEG C of d of solid fermentation 4 that Roots of Peanut was activated are inoculated with the dregs of rice；Culture medium after fermentation adds 40ml steaming
After distilled water, 34 DEG C of concussion enzymolysis 1.5h, 23 DEG C of concussion enzymolysis 10h；100 DEG C of min of destroy the enzyme treatment 15 of enzymolysis liquid, are cooled to room temperature
Afterwards 10 min are centrifuged with supercentrifuge 10000rpm；The supernatant that centrifugation is obtained is spray-dried, and pan feeding temperature is 50
DEG C, EAT is 180 DEG C, and intake is 22h, and peanut protein peptide is obtained after the completion of drying.Peanut protein peptide yield is
89.43%, aflatoxin B in peanut protein peptide1、B2、G1、G2Do not detected with cyclopiazonic acid.The anti-oxidation peptide it is anti-oxidant
Activity is as follows：Remove the IC of DPPH free radicals50It is worth for 8.63mg/mL, the IC of scavenging hydroxyl50It is worth for 3.59mg/mL, clearly
Except the IC of ultra-oxygen anion free radical50It is worth for 5.08mg/mL, peanut antioxidant peptide needed for when the light absorption value of iron reducing power is 0.5
Concentration be 6.07mg/mL, the concentration of peanut antioxidant peptide needed for when the light absorption value of molybdenum reducing power is 0.5 is 4.81mg/mL, iron
The IC of ion chelating power50It is worth for 4.97mg/mL, the IC of chelating copper ions power50It is worth for 3.08mg/mL, suppression linoleic acid peroxidation
The IC of ability50It is worth for the IC of 4.04mg/mL anti-lipid peroxidation abilities50It is worth for 4.05mg/mL.
Control experiment：Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/kg
.10g peanut meals add 20ml water, and sterilizing is inoculated with the bacillus subtilis bacterium that peanut meal activated in water solution is crossed into peanut meal
Plant 0.7 mL, 32 DEG C of d of solid fermentation 4, then the aspergillus niger 0.7g that inoculation Roots of Peanut was activated into peanut meal, 32 DEG C of solids hairs
The d of ferment 4；Culture medium after fermentation is added after 40 mL distilled water, 34 DEG C of concussion enzymolysis 1.5h, 23 DEG C of 10 h of concussion enzymolysis；Enzyme
100 DEG C of min of destroy the enzyme treatment 15 of liquid are solved, is cooled to after room temperature and centrifuges 10 min with supercentrifuge 10000rpm；Centrifugation is obtained
Supernatant be spray-dried, pan feeding temperature be 50 DEG C, EAT be 180 DEG C, intake be 22 h, dry after the completion of
Obtain peanut protein peptide.Peanut protein peptide yield is 60.21%, in peanut protein peptide total aflatoxin content be 98 μ g/kg and
The μ g/kg of cyclopiazonic acid 62.The antioxidation activity of the anti-oxidation peptide is as follows：The IC50 values for removing DPPH free radicals are 11.23
Mg/mL, the IC of scavenging hydroxyl50It is worth for 6.59 mg/mL, the IC of removing ultra-oxygen anion free radical50It is worth for 5.98 mg/
ML, the concentration of peanut antioxidant peptide needed for when the light absorption value of iron reducing power is 0.5 is 6.97 mg/mL, the light absorption value of molybdenum reducing power
The concentration of peanut antioxidant peptide needed for during for 0.5 is 5.91 mg/mL, the IC of iron ion chelating ability50It is worth for 5.97 mg/mL, copper
The IC of ion chelating power50It is worth for 4.18 mg/mL, the IC of suppression linoleic acid peroxidation ability50It is worth and suppresses fat for 4.94 mg/mL
The IC of matter peroxidation capacity50It is worth for 5.75 mg/mL.
Embodiment 3
Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/kg.10g peanut meals add
Enter 25ml water, sterilize, inoculation is through Roots of Peanut fermentation activation rainbow conk strain 0.7g, 28 DEG C of solid fermentation 6d, then to the flower of fermentation
Inoculation peanut meal activated in water solution is crossed in the raw dregs of rice Bacillus subtilis strain 0.7ml, 32 DEG C of d of solid fermentation 4, then to peanut
Aspergillus niger 0.7g, 32 DEG C of d of solid fermentation 4 that Roots of Peanut was activated are inoculated with the dregs of rice；Culture medium after fermentation adds 40ml steaming
After distilled water, 35 DEG C of 1 h of concussion enzymolysis, 22 DEG C of concussion enzymolysis 12h；100 DEG C of min of destroy the enzyme treatment 15 of enzymolysis liquid, are cooled to room temperature
Afterwards 10 min are centrifuged with supercentrifuge 10000rpm；The supernatant that centrifugation is obtained is spray-dried, and pan feeding temperature is 50
DEG C, EAT is 180 DEG C, and intake is 22h, and peanut protein peptide is obtained after the completion of drying.Peanut protein peptide yield is
88.93%, aflatoxin B in peanut protein peptide1、B2、G1、G2Do not detected with cyclopiazonic acid.The anti-oxidation peptide it is anti-oxidant
Activity is as follows：Remove the IC of DPPH free radicals50It is worth for 8.43mg/mL, the IC of scavenging hydroxyl50It is worth for 3.39mg/mL, clearly
Except the IC of ultra-oxygen anion free radical50It is worth for 4.95mg/mL, peanut antioxidant peptide needed for when the light absorption value of iron reducing power is 0.5
Concentration be 5.85mg/mL, the concentration of peanut antioxidant peptide needed for when the light absorption value of molybdenum reducing power is 0.5 is 4.61mg/mL, iron
The IC of ion chelating power50It is worth for 4.57mg/mL, the IC of chelating copper ions power50It is worth for 2.98mg/mL, suppression linoleic acid peroxidation
The IC of ability50It is worth for the IC of 3.94mg/mL anti-lipid peroxidation abilities50It is worth for 3.85mg/mL.
Control experiment：Total aflatoxin content is 92 μ g/kg in peanut meal raw material, and cyclopiazonic acid content is 52 μ g/
kg.10g peanut meals add 25ml water, and sterilizing is inoculated with the bacillus subtilis that peanut meal activated in water solution is crossed into peanut meal
Strain 0.7 mL, 32 DEG C of d of solid fermentation 4, then the aspergillus niger 0.7g that inoculation Roots of Peanut was activated into peanut meal, 32 DEG C of solids
Ferment 4 d；Culture medium after fermentation adds 40 mL distilled water, 22 DEG C of 13 h of concussion enzymolysis；100 DEG C of destroy the enzyme treatments of enzymolysis liquid
15 min, are cooled to after room temperature and centrifuge 10 min with supercentrifuge 10000rpm；The supernatant that centrifugation is obtained is sprayed
Dry, pan feeding temperature is 50 DEG C, EAT is 180 DEG C, intake is 22 h, and peanut protein peptide is obtained after the completion of drying.
Peanut protein peptide yield is 72.53%, and total aflatoxin content is the 23 μ g/kg and μ g/ of cyclopiazonic acid 19 in peanut protein peptide
kg.The antioxidation activity of the anti-oxidation peptide is as follows：Remove the IC of DPPH free radicals50It is worth for 10.43mg/mL, scavenging hydroxyl
IC50It is worth for 5.59mg/mL, the IC of removing ultra-oxygen anion free radical50It is worth for 5.85mg/mL, the light absorption value of iron reducing power is
The concentration of required peanut antioxidant peptide is 7.65 mg/mL when 0.5, and peanut needed for when the light absorption value of molybdenum reducing power is 0.5 is anti-oxidant
The concentration of peptide is 5.81 mg/mL, the IC of iron ion chelating ability50It is worth for 5.87 mg/mL, the IC of chelating copper ions power50It is worth and is
3.91 mg/mL, suppress the IC of linoleic acid peroxidation ability50It is worth for the IC of 4.84 mg/mL anti-lipid peroxidation abilities50Value
For 4.95 mg/mL.
The foregoing is only a specific embodiment of the invention, it is not limited to this, any skill for being familiar with the art
Art personnel the invention discloses technical scope in, change or replacement can be readily occurred in, should all cover the present invention protection model
Within enclosing.
Claims (9)
1. aflatoxin degraded and the synchronous method for preparing high nutrition peanut protein peptide in a kind of peanut meal, its feature include with
Lower step：
（1）Feedstock treating：Peanut meal is crushed, 40 mesh sieves are crossed；A certain amount of distilled water is added into peanut meal powder, is sterilized；
（2）Microorganism is segmented inoculation fermentation：The rainbow conk strain after a certain amount of activation, 25-40 DEG C are inoculated with into the peanut meal of sterilizing
Solid fermentation 5-8 d, then the bacillus subtilis activated, 30-35 DEG C of solid fermentation 3-5 are inoculated with into the peanut meal of fermentation
D, then the aspergillus niger activated, 28-35 DEG C of solid fermentation 3-5 d are inoculated with into peanut meal；During the fermentation, every other day to
The distilled water of certain volume is added in fermentation medium；
（3）Dry, crush：Peanut meal 40-50 after fermentation is dried DEG C, ultramicro grinding, both nontoxic, high nutrition peanut protein
Peptide.
2. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（1）Described peanut meal powder and the adding proportion (g of distilled water:Ml it is) 1:2-1:3.
3. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The activation method of described rainbow conk is：By 6-8 strain plugs（7mm diameters）Manyzoned polypore bacteria
Strain is inoculated in the Roots of Peanut powder that 10 g diameters are about 5 mm, 25-30 DEG C of activation culture 5-7d, and rainbow conk activated spawn is made.
4. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The inoculum concentration of described rainbow conk is：The mass ratio of peanut meal and activated spawn（g:g）For
20:1-20:2。
5. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The activation method of described bacillus subtilis is：Bacillus subtilis is inoculated in
In the peanut meal aqueous solution, Bacillus subtilis strain liquid is made in 25-30 DEG C of activation culture 2-3 d.
6. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The inoculum concentration of described bacillus subtilis is：The quality of peanut meal and activated spawn
Volume ratio（g:ml）For 20:1-20:2.
7. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The activation method of described aspergillus niger is：The Aspergillus niger strain of 2-3 strain rings is inoculated with
In the Roots of Peanut powder that 10 g diameters are about 5mm, 25-30 DEG C of activation culture 3-5d, get processed aspergillus activated spawn.
8. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The inoculum concentration of described aspergillus niger is：The mass ratio of peanut meal and activated spawn（g:g）
For 20:1-20:2.
9. aflatoxin degraded and synchronous preparation high nutrition peanut protein peptide in a kind of peanut meal according to claim 1
Method, it is characterised in that step（2）The addition of distilled water is in described fermentation process：The matter of peanut meal and distilled water
Measuring ratio is：1:1-2:3.
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| CN108102971B (en) * | 2018-01-26 | 2021-04-27 | 山东省花生研究所（山东省农业科学院花生工程技术研究中心） | Pseudomonas monteilii capable of resisting heat and degrading aflatoxin |
| CN108497261A (en) * | 2018-03-01 | 2018-09-07 | 河南农业大学 | A method of removing aflatoxin in biological raw material using strain fermentation |
| CN108967833A (en) * | 2018-08-02 | 2018-12-11 | 合肥工业大学 | A kind of complex enzyme formulation and preparation method thereof for removing aflatoxin |
| CN110583964A (en) * | 2019-09-23 | 2019-12-20 | 江南大学 | Biological removal method for efficiently removing four aflatoxins in peanut meal |
| CN115486513A (en) * | 2021-06-17 | 2022-12-20 | 丰益(上海)生物技术研发中心有限公司 | Method for processing peanut material |
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| CN108034690B (en) | 2020-09-15 |
| CN108034690A (en) | 2018-05-15 |
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