CN108934755A - A method of processing waste residue using pomegranate is primary raw material culture Cordyceps militaris - Google Patents
A method of processing waste residue using pomegranate is primary raw material culture Cordyceps militaris Download PDFInfo
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- CN108934755A CN108934755A CN201810714233.6A CN201810714233A CN108934755A CN 108934755 A CN108934755 A CN 108934755A CN 201810714233 A CN201810714233 A CN 201810714233A CN 108934755 A CN108934755 A CN 108934755A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
It is the method for primary raw material culture Cordyceps militaris that the invention discloses a kind of using pomegranate processing waste residue, the primary raw material using the waste residue after the juicing of pomegranate processing factory as fermentation medium, while establishing scientific and rational cultural method；The obtained Cordyceps militaris of cultural method of the present invention has yield high compared with rice culture, and main active content is high, and the high feature of antioxidant activity realizes the high value added utilization of pomegranate slag waste material.
Description
Technical field
The invention belongs to fruit processing waste technical field of solid fermentation, and in particular to a kind of to be using pomegranate processing waste residue
The method of primary raw material culture Cordyceps militaris.
Technical background
The utilization of pomegranate is also used to the processing of fruit juice product, can generate very in process other than fresh food at present
More wastes --- pomegranate slag (skin seed mixture), not only causes the serious waste of agricultural resource, but also may produce
Raw problem of environmental pollution.Research shows that containing higher tannin, tree fat, protein, soluble sugar, mannitol etc. in granatum
How substance makes full use of the processing waste material of this part, is one containing ingredients such as higher protein, sugar, steroidals in pomegranate seed
A good problem to study.
Cordyceps militaris and cordyceps sinensis is equal in terms of biology belongs to, is the representative species of this category.In terms of materia medica, two
Person's effect is also close, therefore " northern Chinese caterpillar Fungus " is recorded as in pharmacopeia.The hundreds of thousands of members of cordyceps sinensis market price per kilogram, and pupa worm
Careless relative low price.Therefore, the culture of Cordyceps militaris receives significant attention, and does not so use stone still in Artificial Cultivation of Cordyceps militaris Link at present
Report of the pomegranate slag as primary raw material culture.
Summary of the invention
The object of the present invention is to provide a kind of method using pomegranate processing waste residue for primary raw material culture Cordyceps militaris, the party
Primary raw material of the method using the waste residue after the juicing of pomegranate processing factory as fermentation medium, while establishing scientific and rational training
The method of supporting；The obtained Cordyceps militaris of cultural method of the present invention has yield high compared with rice culture, and main active content is high, anti-oxidant
The high feature of activity, realizes the high value added utilization of pomegranate slag waste material.
In recent years, this seminar is the cooperation Huaibei when Fructus Fici tikouae secondary industry and edible mushroom solid culture industry, is carried out
It is the research of primary raw material culture edible mushroom with pomegranate slag.
The present invention is the method for primary raw material culture Cordyceps militaris using pomegranate processing waste residue, is included the following steps:
Step 1: the preparation of Cordyceps militaris seed culture medium
The formula of Cordyceps militaris seed culture medium is as follows: 10~20g/L of corn flour, 10~20g/L of glucose, and bean cake powder 5~
15g/L, yeast extract 5~10g/L, KH2PO41~3g/L, MgSO4·7H20.5~1.5g/L of O, solvent are water；By above-mentioned ratio
Example deploys and adjusts pH value to 5.0~6.5 with NaOH solution after mixing.
Step 2: the preparation of pomegranate residue culture medium
Fresh pomegranate juicing waste residue is taken, with filter-cloth filtering control water content 20~25%；By 40~60 mass parts stones
Pomegranate juicing waste residue, 10~30 mass parts Cordyceps militaris seed culture mediums and 2~5 mass parts dried silkworm chrysalis meals are uniformly mixed, and are respectively charged into straight
Diameter 10cm, high 12cm culture bottle in, admission space be culture bottle volume 1/4~1/3, culture bottle is then put into steam and is gone out
In 115~125 DEG C of 15~25min of sterilizing in bacterium pot, it is cooled to room temperature, it is spare；
Step 3: the preparation of liquid seeds
It takes cordyceps mycelium (commercially available to obtain) to be inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium from inclined-plane, sets
In 24~28 DEG C of constant-temperature tables, with 120~180rpm of revolving speed culture 72~120 hours, level liquid seed is prepared；It will
The level liquid seed is inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium, is placed in 24~28 DEG C of constant-temperature tables
In, with 120~180rpm of revolving speed culture 48~96 hours, prepare secondary liquid seed；
Inoculation ratio in step 3, when cordyceps mycelium is inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium
Example is 1 square centimeter of cordyceps mycelium/50ml Cordyceps militaris seed culture medium；Level liquid seed is inoculated into equipped with Cordyceps militaris
Inoculative proportion when in the triangular flask of seed culture medium is 10% by volume.
Step 4: the culture of fructification
4a, mycelial growth phase: the pomegranate waste residue culture that the secondary liquid seed access step 2 that step 3 obtains is obtained
In base, the inoculation volume of secondary liquid seed is the 2~5% of culture volume, is transferred to and is protected from light culturing room's culture 10~15 days,
18~24 DEG C of cultivation temperature, air humidity 65~80%；
4b, sporophore growth phase: step 4a are transferred to natral light cahure room after cultivating, day temperature is controlled 24
~28 DEG C, at 14~20 DEG C, air humidity is controlled 80~90% the control of evening temperature；After culture is grown to Cordyceps militaris stroma,
It is transferred to light filling culturing room to be cultivated, illuminance is controlled in 250~300LX, daily illumination 10~14 hours, temperature 24~28
DEG C, air humidity 75~85%；
Step 5: harvesting
The mature fruiting bodies of cordyceps militaris for growing into 8~10cm long is collected from culture medium, in 80~90 DEG C of baking ovens
Middle drying 2~3 hours, obtains final products.
Compared with prior art the invention has the advantages that:
1, the present invention can not only alleviate discarded pomegranate using pomegranate slag waste material as primary raw material culture fruiting bodies of cordyceps militaris
Pollution of the slag to environment, more important is be translated into the high fruiting bodies of cordyceps militaris of added value.
2, the present invention cultivates Cordyceps militaris with pomegranate slag is main base starting material, and Cordyceps militaris absorbs pomegranate in growth period
The ingredient of culture medium is above cordycepin content, Thick many candies content, every bottle of yield and the antioxidant activity of fruiting bodies of cordyceps militaris
Produced Cordyceps militaris after using rice as primary raw material substitution.
3, culture medium prescription of the present invention is simple, simple process, can substitute rice as culture medium raw material, there is higher warp
Benefit of helping and social benefit.
Detailed description of the invention
Fig. 1 is to remove energy in embodiment 1 with the DPPH of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Power compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Fig. 2 is in embodiment 1, with the superoxide anion of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Scavenging activity compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Fig. 3 is to remove energy in embodiment 2 with the DPPH of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Power compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Fig. 4 is in embodiment 2, with the superoxide anion of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Scavenging activity compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Fig. 5 is to remove energy in embodiment 3 with the DPPH of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Power compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Fig. 6 is in embodiment 3, with the superoxide anion of pomegranate slag and the Cordyceps militaris extract of rice substitution pomegranate slag culture
Scavenging activity compares；Wherein a is the fructification that pomegranate slag obtains, and b is the fructification that rice obtains.
Specific embodiment
The measurement of cordycepin content is with reference to " the measurement high performance liquid chromatography DB32/T1275 of cordycepin content in Cordyceps militaris
~2008 ".
The extraction of fructification Thick many candies and determination of polysaccharide refer to " Pharmacopoeia of People's Republic of China 2005 editions ".
The measurement of antioxidant activity: (1) Cordyceps militaris is crushed, and by solid-liquid ratio 1:5 extracting in water, is extracted three times, is merged
Extracting solution is freeze-dried after concentration, obtains Cordyceps militaris water extract；Water extract accurately is weighed, is sufficiently dissolved, is made with appropriate distilled water
Concentration is the mother liquor A of 10mg/ml.Take mother liquor A dilute, concentration is respectively the dilution of 8,6,4,2mg/ml, number respectively 1.~
⑤。
(a) measurement of DPPH Scavenging activity:
Each 2ml of dilution of accurate measuring various concentration is respectively 2.0 × 10 with 2ml concentration respectively-4The DPPH of mol/L is molten
Liquid mixing, is placed 30 minutes after shaking up, and is control with corresponding solvent, is measured respectively with ultraviolet-uisible spectrophotometer above-mentioned
Solution respectively obtains Ai value in the absorbance (Ai) that wavelength is at 517nm.The dilution of accurate measuring various concentration is each respectively
Solvent corresponding with 2ml after mixing, is control with solvent, is measured respectively with ultraviolet-uisible spectrophotometer 2ml respectively
Above-mentioned solution respectively obtains Aj value in the absorbance (Aj) that wavelength is at 517nm.Respectively accurate measuring 2ml concentration be 2.0 ×
10-4The DPPH solution of mol/L is mixed with distilled water, is control with corresponding solvent, is distinguished with ultraviolet-uisible spectrophotometer
It is the absorbance (Ac) at 517nm that above-mentioned solution, which is measured, in wavelength.DPPH Scavenging activity calculation formula are as follows: [1- (Ai-Aj)/Ac]
×100
(b) measurement of superoxide anion Scavenging activity:
Trishydroxymethylaminomethane-hydrochloric acid buffer solution (pH8.2) 4.5ml for taking 0.05mol/L, sets in 25 DEG C of water-baths
Preheating after twenty minutes, is separately added into the sample solution and 0.4ml 25mmol/L pyrogallol solution of 1ml various concentration, after mixing
It is reacted 1 minute in 25 DEG C of water-baths, 8mmol/L HCl 1ml is added and terminates reaction, 299nm under ultraviolet-visible spectrophotometer
The absorbance (Ai) of place's measurement solution, respectively obtains Ai value.Blank control group replaces sample solution with the distilled water of same volume,
The absorbance (A0) for measuring solution at 299nm under ultraviolet-uisible spectrophotometer, respectively obtains A0 value.Superoxide anion is removed
Capacity calculation formula are as follows: (A0-Ai)/A0 × 100.
The present invention is further illustrated below by way of specific embodiment.
Cordyceps mycelium used in the embodiment of the present invention is purchased from the U.S. edible mushroom Professional Cooperation of the strong that in the port Dongtai City Jianggang town
Society.
Embodiment 1:
It is as follows using the method that pomegranate processing waste residue is primary raw material culture Cordyceps militaris in the present embodiment:
1, the preparation of Cordyceps militaris seed culture medium
The formula of Cordyceps militaris seed culture medium is as follows: corn flour 20g/L, glucose 20g/L, bean cake powder 15g/L, yeast extract
10g/L, KH2PO43g/L, MgSO4·7H2O 1.5g/L, solvent are water；NaOH solution is used after deploying and mix according to the above ratio
PH value is adjusted to 6.5.
2, the preparation of pomegranate residue culture medium
(Soluble adhesion molecule is greater than 24% to the pomegranate juicing waste residue for taking Huaibei City guava juice factory fresh, crude protein
Content is greater than 11%, and 9%) crude fat content is greater than, with filter-cloth filtering control water content 20~25%；By 40 mass parts pomegranates
Juicing waste residue, 10 mass parts Cordyceps militaris seed culture mediums and 2 mass parts dried silkworm chrysalis meals are uniformly mixed, and are respectively charged into diameter 10cm, height
In the culture bottle of 12cm, admission space be culture bottle volume 1/4~1/3, then by culture bottle be put into steam sterilization pan in
115 DEG C of sterilizing 15min, are cooled to room temperature, spare；
3, the preparation of liquid seeds
Cordyceps mycelium is taken to be inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium from preservation inclined-plane
In, inoculative proportion is 1 square centimeter of cordyceps mycelium/50ml Cordyceps militaris seed culture medium, it is placed in 24 DEG C of constant-temperature tables, with
Revolving speed 120rpm is cultivated 72 hours, prepares level liquid seed；By the level liquid seed by volume 5% ratio
It is inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium, inoculative proportion is 10% by volume, is placed in
In 24 DEG C of constant-temperature tables, with revolving speed 120rpm culture 48 hours, secondary liquid seed is prepared；
4, the culture of fructification
4a, mycelial growth phase: the secondary liquid seed that step 3 obtains is obtained by 2% volume ratio access step 2
In pomegranate residue culture medium, it is transferred to and is protected from light culturing room's culture 10 days, 18~24 DEG C of cultivation temperature, air humidity 65~80%；
4b, sporophore growth phase: step 4a are transferred to natral light cahure room after cultivating, day temperature is controlled 24
~28 DEG C, at 14~20 DEG C, air humidity is controlled 80~90% the control of evening temperature；After culture is grown to Cordyceps militaris stroma,
Light filling culturing room is transferred to be cultivated, illuminance is controlled in 250~300LX, daily illumination 10 hours, temperature at 24~28 DEG C,
Air humidity 75~85%；
5, it harvests
The mature fruiting bodies of cordyceps militaris for growing into 8~10cm long is collected from culture medium, in 80~90 DEG C of baking ovens
Middle drying 2 hours, obtains final products.
The present embodiment compares the yield of pomegranate slag and rice substitution the obtained fructification of pomegranate slag and main active contains
It measures (being shown in Table 1), and to their obtained Cordyceps militaris water extract measurements antioxidant activity (see Fig. 1, Fig. 2).It can by table 1, Fig. 1 and Fig. 2
Know, is superior to rice by the Cordyceps militaris yield of primary raw material, cordycepin content, Thick many candies content, antioxidant activity of pomegranate slag
Substitute the obtained Cordyceps militaris of pomegranate slag.
In 1 embodiment 1 of table, with the Cordyceps militaris index of pomegranate slag and rice substitution pomegranate slag culture
Embodiment 2:
It is as follows using the method that pomegranate processing waste residue is primary raw material culture Cordyceps militaris in the present embodiment:
1, the preparation of Cordyceps militaris seed culture medium
The formula of Cordyceps militaris seed culture medium is as follows: corn flour 10g/L, glucose 10g/L, bean cake powder 5g/L, yeast extract
5g/L, KH2PO41g/L, MgSO4·7H2O 0.5g/L, solvent are water；NaOH solution is used after deploying and mix according to the above ratio
PH value is adjusted to 5.0.
2, the preparation of pomegranate residue culture medium
(Soluble adhesion molecule is greater than 24% to the pomegranate juicing waste residue for taking Huaibei City guava juice factory fresh, crude protein
Content is greater than 11%, and 9%) crude fat content is greater than, with filter-cloth filtering control water content 20~25%；By 60 mass parts pomegranates
Juicing waste residue, 30 mass parts Cordyceps militaris seed culture mediums and 5 mass parts dried silkworm chrysalis meals are uniformly mixed, and are respectively charged into diameter 10cm, height
In the culture bottle of 12cm, admission space be culture bottle volume 1/4~1/3, then by culture bottle be put into steam sterilization pan in
125 DEG C of sterilizing 25min, are cooled to room temperature, spare；
3, the preparation of liquid seeds
Cordyceps mycelium is taken to be inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium from preservation inclined-plane
In, inoculative proportion is 1 square centimeter of cordyceps mycelium/50ml Cordyceps militaris seed culture medium, it is placed in 28 DEG C of constant-temperature tables, with
Revolving speed 180rpm is cultivated 120 hours, prepares level liquid seed；By the level liquid seed by volume 5% ratio
Example is inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium, and inoculative proportion is 10% by volume, juxtaposition
In 28 DEG C of constant-temperature tables, with revolving speed 180rpm culture 96 hours, secondary liquid seed is prepared；
4, the culture of fructification
4a, mycelial growth phase: the secondary liquid seed that step 3 obtains is obtained by 5% volume ratio access step 2
In pomegranate residue culture medium, it is transferred to and is protected from light culturing room's culture 15 days, 18~24 DEG C of cultivation temperature, air humidity 65~80%；
4b, sporophore growth phase: step 4a are transferred to natral light cahure room after cultivating, day temperature is controlled 24
~28 DEG C, at 14~20 DEG C, air humidity is controlled 80~90% the control of evening temperature；After culture is grown to Cordyceps militaris stroma,
Light filling culturing room is transferred to be cultivated, illuminance is controlled in 250~300LX, daily illumination 14 hours, temperature at 24~28 DEG C,
Air humidity 75~85%；
5, it harvests
The mature fruiting bodies of cordyceps militaris for growing into 8~10cm long is collected from culture medium, is done in 90 DEG C of baking ovens
Dry 3 hours, obtain final products.
The present embodiment compares the yield of pomegranate slag and rice substitution the obtained fructification of pomegranate slag and main active contains
It measures (being shown in Table 2), and to their obtained Cordyceps militaris water extract measurements antioxidant activity (see Fig. 3, Fig. 4).It can by table 2, Fig. 3 and Fig. 4
Know, is superior to rice by the Cordyceps militaris yield of primary raw material, cordycepin content, Thick many candies content, antioxidant activity of pomegranate slag
Substitute the obtained Cordyceps militaris of pomegranate slag.
In 2 embodiment 2 of table, with the Cordyceps militaris index of pomegranate slag and rice substitution pomegranate slag culture
Embodiment 3:
It is as follows using the method that pomegranate processing waste residue is primary raw material culture Cordyceps militaris in the present embodiment:
1, the preparation of Cordyceps militaris seed culture medium
The formula of Cordyceps militaris seed culture medium is as follows: corn flour 15g/L, glucose 15g/L, bean cake powder 10g/L, yeast extract
7.5g/L, KH2PO42g/L, MgSO4·7H2O 1g/L, solvent are water；NaOH solution is used after deploying and mix according to the above ratio
PH value is adjusted to 6.0.
2, the preparation of pomegranate residue culture medium
(Soluble adhesion molecule is greater than 24% to the pomegranate juicing waste residue for taking Huaibei City guava juice factory fresh, crude protein
Content is greater than 11%, and 9%) crude fat content is greater than, with filter-cloth filtering control water content 20~25%；By 50 mass parts pomegranates
Juicing waste residue, 20 mass parts Cordyceps militaris seed culture mediums and 4 mass parts dried silkworm chrysalis meals are uniformly mixed, and are respectively charged into diameter 10cm, height
In the culture bottle of 12cm, admission space be culture bottle volume 1/4~1/3, then by culture bottle be put into steam sterilization pan in
120 DEG C of sterilizing 25min, are cooled to room temperature, spare；
3, the preparation of liquid seeds
Cordyceps mycelium is taken to be inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium from preservation inclined-plane
In, inoculative proportion is 1 square centimeter of cordyceps mycelium/50ml Cordyceps militaris seed culture medium, it is placed in 26 DEG C of constant-temperature tables, with
Revolving speed 150rpm is cultivated 96 hours, prepares level liquid seed；By the level liquid seed by volume 5% ratio
It is inoculated into the 250mL triangular flask equipped with 100mL Cordyceps militaris seed culture medium, inoculative proportion is 10% by volume, is placed in
In 26 DEG C of constant-temperature tables, with revolving speed 150rpm culture 72 hours, secondary liquid seed is prepared；
4, the culture of fructification
4a, mycelial growth phase: the secondary liquid seed that step 3 obtains is obtained by 4% volume ratio access step 2
In pomegranate residue culture medium, it is transferred to and is protected from light culturing room's culture 12 days, 18~24 DEG C of cultivation temperature, air humidity 65~80%；
4b, sporophore growth phase: step 4a are transferred to natral light cahure room after cultivating, day temperature is controlled 24
~28 DEG C, at 14~20 DEG C, air humidity is controlled 80~90% the control of evening temperature；After culture is grown to Cordyceps militaris stroma,
Light filling culturing room is transferred to be cultivated, illuminance is controlled in 250~300LX, daily illumination 12 hours, temperature at 24~28 DEG C,
Air humidity 75~85%；
5, it harvests
The mature fruiting bodies of cordyceps militaris for growing into 8~10cm long is collected from culture medium, is done in 85 DEG C of baking ovens
Dry 2.5 hours, obtain final products.
The present embodiment compares the yield of pomegranate slag and rice substitution the obtained fructification of pomegranate slag and main active contains
It measures (being shown in Table 3), and to their obtained Cordyceps militaris water extract measurements antioxidant activity (see Fig. 5, Fig. 6).It can by table 3, Fig. 5 and Fig. 6
Know, is superior to rice by the Cordyceps militaris yield of primary raw material, cordycepin content, Thick many candies content, antioxidant activity of pomegranate slag
Substitute the obtained Cordyceps militaris of pomegranate slag.
In 3 embodiment 3 of table, with the Cordyceps militaris index of pomegranate slag and rice substitution pomegranate slag culture
Claims (10)
1. a kind of process the method that waste residue is primary raw material culture Cordyceps militaris using pomegranate, it is characterised in that include the following steps:
Step 1: the preparation of Cordyceps militaris seed culture medium
The formula of Cordyceps militaris seed culture medium is as follows: 10~20g/L of corn flour, 10~20g/L of glucose, 5~15g/ of bean cake powder
L, yeast extract 5~10g/L, KH2PO41~3g/L, MgSO4·7H20.5~1.5g/L of O, solvent are water；It adjusts according to the above ratio
PH value is adjusted to 5.0~6.5 with NaOH solution after matching and mixing.
Step 2: the preparation of pomegranate residue culture medium
Fresh pomegranate juicing waste residue is taken, with filter-cloth filtering control water content 20~25%；By pomegranate juicing waste residue, Cordyceps militaris
Seed culture medium and dried silkworm chrysalis meal are uniformly mixed, and are respectively charged into the culture bottle of diameter 10cm, high 12cm, are then put into culture bottle
It sterilizes, is cooled to room temperature in steam sterilization pan, it is spare；
Step 3: the preparation of liquid seeds
It takes cordyceps mycelium to be inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium from inclined-plane, is placed in constant-temperature table,
It is cultivated with 120~180rpm of revolving speed and obtains level liquid seed；The level liquid seed is inoculated into equipped with Cordyceps militaris seed
It in the triangular flask of culture medium, is placed in constant-temperature table, is cultivated with 120~180rpm of revolving speed and obtain secondary liquid seed；
Step 4: the culture of fructification
4a, mycelial growth phase: in the pomegranate residue culture medium that the secondary liquid seed access step 2 that step 3 obtains is obtained,
It is transferred to and is protected from light culturing room's culture；
4b, sporophore growth phase: step 4a are transferred to natral light cahure room after cultivating and are cultivated, culture to Cordyceps militaris
After stroma is grown, it is transferred to light filling culturing room and is cultivated；
Step 5: harvesting
The mature fruiting bodies of cordyceps militaris for growing into 8~10cm long is collected from culture medium, is done in 80~90 DEG C of baking ovens
Dry 2~3 hours, obtain final products.
2. according to the method described in claim 1, it is characterized by:
In step 2, by 40~60 mass parts pomegranates juicing waste residue, 10~30 mass parts Cordyceps militaris seed culture mediums and 2~5 mass
Part dried silkworm chrysalis meal is uniformly mixed.
3. according to the method described in claim 1, it is characterized by:
In step 2, the admission space of culture bottle is the 1/4~1/3 of culture bottle volume.
4. according to the method described in claim 1, it is characterized by:
In step 2, sterilising temp is 115~125 DEG C, and sterilization time is 15~25min.
5. according to the method described in claim 1, it is characterized by:
In step 3, inoculative proportion when cordyceps mycelium is inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium is 1
Square centimeter cordyceps mycelium/50ml Cordyceps militaris seed culture medium.
6. according to the method described in claim 1, it is characterized by:
In step 3, inoculative proportion when level liquid seed is inoculated into the triangular flask equipped with Cordyceps militaris seed culture medium by
Volume ratio is 10%.
7. according to the method described in claim 1, it is characterized by:
In step 3, the cultivation temperature of level liquid seed is 24~28 DEG C, and incubation time is 72~120 hours；Secondary liquid kind
The cultivation temperature of son is 24~28 DEG C, and incubation time is 48~96 hours.
8. according to the method described in claim 1, it is characterized by:
In step 4a, the inoculation volume of secondary liquid seed is the 2~5% of culture volume.
9. according to the method described in claim 1, it is characterized by:
In step 4a, it is protected from light 18~24 DEG C of cultivation temperature of culturing room, air humidity 65~80%, incubation time 10~15 days.
10. according to the method described in claim 1, it is characterized by:
In step 4b, when natral light cahure room is cultivated, at 24~28 DEG C, evening temperature is controlled 14~20 for day temperature control
DEG C, air humidity is controlled 80~90%；When light filling culturing room cultivates, illuminance is in 250~300LX, daily illumination 10~14
Hour, temperature control is at 24~28 DEG C, air humidity 75~85%.
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| CN104108994A (en) * | 2014-06-23 | 2014-10-22 | 铜陵市美雨菌业有限责任公司 | Mushroom medium by taking poplar bark as raw material and preparation method thereof |
| CN104969773A (en) * | 2015-06-09 | 2015-10-14 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for acquiring fermented product or fruiting body of Cordyceps militaris with fermentation of sweet potato residues |
| CN106613956A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Bacteriostatic stevia rebaudiana culture medium |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
| CN107624509A (en) * | 2017-11-06 | 2018-01-26 | 太湖县金江源农业发展有限公司 | Elegant precious mushroom cultivation culture matrix and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104108994A (en) * | 2014-06-23 | 2014-10-22 | 铜陵市美雨菌业有限责任公司 | Mushroom medium by taking poplar bark as raw material and preparation method thereof |
| CN104969773A (en) * | 2015-06-09 | 2015-10-14 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for acquiring fermented product or fruiting body of Cordyceps militaris with fermentation of sweet potato residues |
| CN106613956A (en) * | 2016-11-03 | 2017-05-10 | 明光市大全甜叶菊专业合作社 | Bacteriostatic stevia rebaudiana culture medium |
| CN107333568A (en) * | 2017-09-19 | 2017-11-10 | 张家港市藏联生物研究所有限公司 | A kind of more batches of culture techniques of Cordyceps militaris constant temperature |
| CN107624509A (en) * | 2017-11-06 | 2018-01-26 | 太湖县金江源农业发展有限公司 | Elegant precious mushroom cultivation culture matrix and preparation method thereof |
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