CN108575550A - A kind of method of artificial culture meat cordyceps sinensis - Google Patents
A kind of method of artificial culture meat cordyceps sinensis Download PDFInfo
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- CN108575550A CN108575550A CN201810216040.8A CN201810216040A CN108575550A CN 108575550 A CN108575550 A CN 108575550A CN 201810216040 A CN201810216040 A CN 201810216040A CN 108575550 A CN108575550 A CN 108575550A
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- meat
- cordyceps sinensis
- cordyceps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of methods of artificial culture meat cordyceps sinensis, which is characterized in that using livestock meat as the solid medium of artificial culture cordyceps sinensis, produces the meat cordyceps sinensis that meat cordyceps militaris sporocarp and meat culture medium of Cordyceps militaris are integrally unified.The present invention has imitated the habit that wild cordyceps are had a meat diet (i.e. polypide or pupal cell are nutrition), the solid medium produced using livestock and poultry meat as artificial aweto, the method of being a vegetarian in the past with rice, wheat etc. for solid medium is overturned, the nutritional ingredient for the meat cordyceps sinensis produced, better than the cordyceps sinensis for production of being a vegetarian, edible medicinal value is suitable with wild Chinese caterpillar fungus, can solve the increasingly deficienter situation of wild Chinese caterpillar fungus resource completely.Factorial praluction for meat cordyceps sinensis is laid a good foundation, and also for the comprehensive development and utilizations such as medicinal, edible of meat cordyceps sinensis and is carried out the Active Components of meat cordyceps sinensis herb and is detached with extraction and open new approach.
Description
Technical field
The present invention relates to a kind of methods of artificial culture meat cordyceps sinensis, and in particular to a kind of to use livestock meat as solid culture
Base turns out the cultural method for the meat cordyceps sinensis that worm (meat culture body) careless (fructification) is integrally unified, and belongs to the artificial worm of edible and medical fungi
The technical field of careless cultural method.
Background technology
The technology of artificial large-scale industrialized production cordyceps militaris sporocarp is ripe, but the solid training that these technologies use
Foster base is all with plant base (rice or wheat) for primary raw material, that is to say, that artificial aweto, which is a vegetarian, grows up, this and worm
Grass is grown in the difference had a meat diet and grown up on polypide or pupal cell in a natural environment；Moreover, the worm grass product manually produced is
Fructification, that is, so-called grass, what is grown under natural environment is the entirety of polypide and cursive script unification, and the two entity is also different.Institute
Stating conclusion can find from following patent of invention：
Examining an antifatigue solid-state winter worm of Chinese invention patent CN201510687656.X ＜ anticancers in disclosure
Summer grass volvatus powder production technology>Cordyceps sinensis special solid culture substrate is required in claim 3, is by based on rice, silkworm chrysalis
Want raw material：The antifatigue solid-state Chinese caterpillar fungus bacterium powder production technology of claim 3 anticancer, it is characterized in that：Cordyceps sinensis special solid
It is primary raw material that culture substrate, which is by rice, silkworm chrysalis, and optionally it is a kind of or two kinds are combined into solid state rheology matrix.It is pressed 1:
1-1.5 times or so addition cordyceps sinensis special nutrient fluid, it is that cordyceps sinensis is special solid to sterilize at high temperature after bottling 2-3 hours
State culture medium.
Examining a Chinese invention patent CN201610300480.2 in disclosure《A kind of white Cordyceps militaris production work
Skill》Claim 2 in step c and 4 in requirement with rice make culture medium：Culture medium, material-water ratio 1 are made with rice：
PH value is adjusted to 7.0-8.0 by 1.2-1.8 with water, the high-temperature sterilization 30min at 121 DEG C, spare after cooling.
Examining a Chinese invention patent CN201610262936.0 in disclosure《A kind of pupa preventing living contaminants
Cordyceps militaris sporocarp cultural method》It requires to cultivate Cordyceps militaris on the mixture of rice, wheat or both in fact in claim 1
Body：Cordyceps militaris culture is to use the mixture that 20 portions of rice, wheats or both are packed into flint glass bottle, by 1: 1.6-1.8 material
Water is than being added nutrient solution；It is opened immediately after cooling resistance in 118-121 DEG C, 30min high pressure sterilizations after being sealed with high-temperature resistance plastice bag
High temperature plastics bag carries out inoculation Cordyceps militaris parent species ... ...
An authorized Chinese invention patent CN201510267036.0《A kind of special parent species of Cordyceps militaris production
Preparation method》Rice is used in solid-state plant formulation in the cultivation of 3 fructification of embodiment：
Embodiment 3：Fructification cultivates solid-state plant formulation：Rice (30g)+nutrient solution 35mL...., it sterilizes, sterilizing
Bottle is taken out afterwards to be cooled down, and is inoculated with.
An authorized Chinese invention patent CN201410399184.3《A kind of Cordceps militaris industrial artificial cultivation side
Method》Claim 1. (2) b, culture medium are black rice, black soya bean slag, triticale, mushroom slag etc.：Indoor growing b, culture medium are matched
System, culture medium prescription：0.5 kilogram of black rice, 0.2 kilogram of black soya bean slag, 0.1 kilogram of triticale, 0.5 kilogram of mushroom slag ... ...
An authorized Chinese invention patent CN201210180691.9《A kind of artificial culture swinging seat cordyceps militaris sporocarp
Method》It requires to use wheat groat as solid medium in step C in claim 1：Cultural hypha：In 400ml containers
Middle addition 20g wheat groats, by solid-liquid ratio 1：Fructification cultivating nutrient solution is added in 2 ratio, stirs evenly, and seals, and it is small to impregnate 2
When, 121 DEG C of high-temperature sterilization 20min of temperature, natural cooling obtains solid medium.
An authorized Chinese invention patent CN201110006137.4《A kind of artificial culture Taiwan cordyceps militaris sporocarp
Method》Prepared by the step C in claim 1 is rice medium：20g rice is added in can clean 350ml,
By material-water ratio 1：1.8~1：Can solid mycelia culture solution is added in 2.0 ratio, and pH value is 4~6, is stirred evenly, with resistance to height
The covering of warm polypropylene sealed membran, outside are bandaged with newspaper, are impregnated 2 hours, in 121 DEG C of high-temperature sterilization 30min, natural cooling,
Obtain rice medium；……
Above-mentioned patent be all using plant as solid medium culture artificial aweto, produce only fructification, this with
Naturally the case where growing is completely different；And after artificial aweto harvesting, solid medium almost no longer utilizes.Cordyceps Militaris nature shape
It is grown under condition on solid medium as polypide or pupal cell, that is to say, that natural cordyceps sinensis, which has a meat diet, to grow up, people
The entirety unified using polypide and fructification.Although document has been reported that, artificial aweto and the cordyceps sinensis herb that grows naturally
Many nutritional ingredients and its content are essentially identical, the clinical research of medicine also have confirmation they the effect of essentially identical, but people
The cordyceps sinensis and what is grown naturally have a meat diet cordyceps sinensis in terms of nutritive value and medical value of being a vegetarian of work production, since nobody is from artificial
The meat or fish of cordyceps sinensis solid medium or the angle of element set out and carry out research and technology creation, so being difficult to carry out substantive comparison.Again
Have and be exactly, the fructification that Cordyceps Sinensis From Tibet bacterium can not achieve scale so far is manually cultivated, and whether this has with solid medium
Pass needs further to be studied.
Simulation nature cordyceps sinensis has a meat diet the habit of growth, realizes the people that large-scale industrialized production has worm to have grass integrally to unify
Work cordyceps sinensis, the maximum medicinal and edible value for improving Cordyceps Militaris are to develop and use Cordyceps Militaris to be allowed to preferably by human use
Scientific research and technical task in the urgent need to address.
Invention content
Problem to be solved by this invention is：A kind of method of artificial culture meat cordyceps sinensis is provided, using livestock meat as artificial
Cultivate the solid medium of cordyceps sinensis.
To solve the above-mentioned problems, the present invention provides a kind of methods of artificial culture meat cordyceps sinensis, which is characterized in that will raise
Solid medium of the poultry as artificial culture cordyceps sinensis, produces the meat that meat cordyceps militaris sporocarp and meat culture medium of Cordyceps militaris are integrally unified
Cordyceps sinensis.
Preferably, the above method includes the following steps：
Step 1)：The meat cordyceps sinensis production preparation of liquid spawn：
Cordyceps sinensis wild-type strain is acquired, routine culture acquisition meat cordyceps sinensis production is carried out to it and uses liquid spawn, it is spare；
Step 2)：The preparation of meat cordyceps sinensis solid medium：
It by fresh livestock meat, cleans, peeling is boned, chopping, and sugar is added and salt is uniformly mixed, crushes, is stood after crushing
It is packed into culture vessel and carries out steam high-temperature sterilization, meat cordyceps sinensis solid medium is made in high-temperature sterilization postcooling to room temperature, turns
It is for use to move to transfer room；
Step 3)：It is inoculated on meat cordyceps sinensis solid medium：
Meat cordyceps sinensis is produced and is inoculated on meat cordyceps sinensis solid medium with liquid spawn with the mode of sowing grass seeds by duster；
Step 4)：The culture of meat cordyceps sinensis：
Culture vessel is placed on culturing rack, the conventional method culture meat cordyceps sinensis being factory produced by cordyceps sinensis；
Step 5)：The harvesting of meat cordyceps sinensis：
When there is molecule on the meat cordyceps militaris sporocarp on meat culture medium of Cordyceps militaris, show that meat cordyceps sinensis enters ripe rank
Section, at this time harvests it.
It is highly preferred that the cordyceps sinensis wild-type strain in the step 1) is aweto strain or Cordyceps militaris spawn.
It is highly preferred that the fresh livestock meat in the step 2) individually uses, or be added meat quality 1% sugar and
0.5% salt crushes together with meat.
It is highly preferred that the harvesting in the step 5) is specially：The meat cordyceps militaris sporocarp to connect together and meat cordyceps sinensis are trained
Foster base is integrally that meat cordyceps sinensis harvests together, or first harvests meat cordyceps militaris sporocarp, then harvests meat culture medium of Cordyceps militaris.
It is highly preferred that the edible medicinal method of the meat cordyceps sinensis of the step 5) harvesting is：Fresh food, refrigeration after eat, be made it is dry
Product or crushed after being dried comprehensive utilization.
Present invention firstly provides the concept of meat cordyceps sinensis, i.e., the meat cordyceps sinensis manually cultivated is by meat cordyceps militaris sporocarp meat and cordyceps sinensis
One entirety of culture medium composition, has worm (polypide or pupal cell) to have grass (to grow the son on worm or pupal cell as wild Chinese caterpillar fungus
Entity), in entirety of the livestock meat as cordyceps militaris sporocarp and the culture medium of Cordyceps militaris unification produced on artificial solid medium, claim
For meat cordyceps sinensis；In livestock meat as the cordyceps militaris sporocarp produced on artificial solid medium, referred to as meat cordyceps militaris sporocarp；It is raiseeing
Poultry is cultivated as the culture medium of Cordyceps militaris after the cordyceps militaris sporocarp harvesting produced on artificial solid medium, referred to as meat cordyceps sinensis
Base.The present invention is also put forward for the first time the concept of " artificial aweto be a vegetarian production method " and " artificial aweto have a meat diet production method ".
The present invention has imitated the habit (i.e. polypide or pupal cell are nutrition) that wild cordyceps have a meat diet, using livestock and poultry meat as
The solid medium of artificial aweto production, has overturned the method for being a vegetarian in the past with rice, wheat etc. for solid medium, has produced
Meat cordyceps sinensis nutritional ingredient, better than the cordyceps sinensis for production of being a vegetarian, edible medicinal value it is suitable with wild Chinese caterpillar fungus, open country can be solved completely
The increasingly deficienter situation of infested grass resource.Factorial praluction for meat cordyceps sinensis is laid a good foundation, and is also medicinal, the food of meat cordyceps sinensis
It is detached with extraction with equal comprehensive development and utilizations and the Active Components for carrying out meat cordyceps sinensis herb and is opened new approach.
Producer of the present invention is simple, and in original " artificial aweto be a vegetarian production method ", meat cordyceps sinensis solid medium is replaced
Rice or wheat are changed as solid medium, forms " artificial aweto have a meat diet production method ", the meat cordyceps sinensis fresh food produced more may be used
Mouthful, meat cordyceps sinensis herb nutritive value and the closer cordyceps sinensis grown naturally of medical value.
Description of the drawings
Fig. 1,2 for artificial culture meat cordyceps sinensis provided by the invention method flow chart.
Specific implementation mode
In order to make the present invention more obvious and understandable, it is hereby described in detail below with preferred embodiment.
Embodiment 1
A kind of method of artificial culture meat cordyceps sinensis：
1. preparing meat cordyceps sinensis production Cordyceps militaris spawn original seed
Northeast wild cordyceps militaris strain is acquired, is separately cultured by the conventional method of cordyceps species culture.
1.1. Cordyceps militaris spawn original seed is prepared
The preparation of isolation medium：100 grams of fresh potato is cut into the fritter less than 1 centimeter square, boiling water boiling 30 minutes,
Filter to take juice；5 grams of stirring and dissolvings of starch are added in filtered juice excessively and add 15 grams of glucose, 10 grams of sucrose, 5 grams of peptones, 3 for elder generation
Gram yeast extract, 1 gram of K2HPO4, 1 gram of KH2PO4With 1 gram of MgSO4, stirring and dissolving, with tap water constant volume to 1000 milliliters；It is separated into small
Bottle is put into sterilizing in autoclave sterilizer, cooling, spare.
Sclerotium (polypide) and stroma separation：The cordyceps sclerotium adopted back and stroma are subjected to surface with 0.1% mercuric chloride liquid
Disinfection, sterile water are cleaned multiple times until not having mercuric chloride；It is then cut into segment or thin slice, the isolation medium being placed in culture dish
On, it is cultivated at 8~18 DEG C, at Cordyceps militaris spawn original seed, is placed in refrigerator and preserves.
1.2. production Cordyceps militaris strain is prepared
The preparation of liquid spawn culture medium：In tap water be added 20 grams of glucose, 10 grams of sucrose, 2 grams of peptones, 2 grams
Yeast extract, 1 gram of K2HPO4, 1 gram of KH2PO4With 1 gram of MgSO4, stirring and dissolving is settled to 1000 milliliters with tap water；It is separated into bottle
It is put into sterilizing in autoclave sterilizer, it is cooling, it is spare at liquid spawn culture medium；
Cordyceps militaris spawn original seed is seeded on the liquid spawn culture medium that sterilizing has cooled down, is expanded on reciprocal shaker
Culture, at production Cordyceps militaris strain.
2. preparing meat cordyceps sinensis solid medium
Fresh pork is cleaned, peeling is boned, chopping；One times of tap water is added, adds 1% sugar and 0.5% salt,
It is uniformly mixed, crushes；It is packed into culture vessel after crushing, meat mincing height 2mm of loading or so, in addition leachy lid, will train
Container is supported to be packed into sterilizer；It is carried out disinfection with high steam, then 0.15MPa 30 minutes blocks steam, wait for pressure nature
When being down to zero, sterilizer is opened, takes out culture vessel, cooled to room temperature, prepared by meat cordyceps sinensis solid medium completes.Meat worm
Careless solid medium need to complete inoculation in 3 hours.
3. being inoculated on meat cordyceps sinensis solid medium
In transfer room, production Cordyceps militaris strain is diluted 8 times with the water to sterilize in the watering can sterilized, so
It uniformly sows grass seeds by duster respectively afterwards on the meat cordyceps sinensis solid medium prepared, that is, completes inoculation；
4. meat cordyceps sinensis is cultivated
The culture vessel for being inoculated with liquid spawn is placed on culturing rack, by cordyceps sinensis be factory produced conventional method into
Row culture：Cultural hypha phase condition controls：Humidity 70%~80%, 22 ± 1 DEG C of temperature, takes the measure of being protected from light；Fructification culture
Stage conditions control：When mycelia is covered with solid medium, into fructification culture, humidity 70%~80%, temperature 25 ± 1
DEG C, light application time 12h illumination 12h or dark alternately, using fluorescent lamp lighting, intensity of illumination 1500lux；
5. the harvesting of meat cordyceps sinensis
Meat cordyceps militaris sporocarp is grown to 5~8 centimeters, when occurring molecule in fructification, is harvested, and meat cordyceps sinensis is real
Body and meat culture medium of Cordyceps militaris connect together, and i.e. meat cordyceps sinensis is whole to be dug out from culture vessel together, is placed.A part first harvests meat
Cordyceps militaris sporocarp, then meat culture medium of Cordyceps militaris is harvested, it places respectively.
6. the edible medicinal of meat cordyceps sinensis
The meat cordyceps sinensis of harvesting can be eaten raw, can also be eaten after refrigeration, eat, can also do after can also harvesting drying
Disintegrating composite utilizes after dry.Pork meat cordyceps militaris sporocarp can be eaten raw immediately, fresh and tender deliciousness, be differed completely with the taste for cordyceps sinensis of being a vegetarian
Sample.Meat cordyceps militaris sporocarp can be eaten raw with meat culture medium of Cordyceps militaris as meat cordyceps sinensis, can also be eaten, can also be adopted after refrigeration
It is eaten after receiving drying, it can also crushed after being dried comprehensive utilization.
Embodiment 2
A kind of method of artificial culture meat cordyceps sinensis：
1. preparing meat cordyceps sinensis production aweto liquid spawn
Xizang Wild aweto strain is acquired, is separately cultured by the conventional method of cordyceps species culture：
1.1. sclerotium (polypide) and stroma separation：By the aweto sclerotium adopted back and stroma with 0.1% mercuric chloride liquid into
Row surface sterilization is cleaned multiple times with sterile water until not having mercuric chloride；It is then cut into segment or thin slice, the separation being placed in culture dish
It on culture medium, is cultivated at 8~18 DEG C, at aweto strain original seed, is placed in refrigerator and preserves.
The preparation of isolation medium：100 grams of fresh potato is cut into the fritter less than 1 centimeter square, boiling water boiling 30 minutes,
Filter to take juice；5 grams of stirring and dissolvings of starch are added in filtered juice excessively and add 15 grams of glucose, 10 grams of sucrose, 5 grams of peptones, 3 for elder generation
Gram yeast extract, 1 gram of K2HPO4, 1 gram of KH2PO4With 1 gram of MgSO4, stirring and dissolving, with tap water constant volume to 1000 milliliters；It is separated into small
Bottle is put into sterilizing in autoclave sterilizer, and cooling is spare.
1.2. production is prepared with cordyceps sinensis liquid spawn：Aweto strain original seed is seeded in the liquid that sterilizing has cooled down
On body bacterium culture medium, expand culture on reciprocal shaker, at production with cordyceps sinensis liquid spawn.
The preparation of liquid spawn culture medium：In tap water be added 15 grams of glucose, 10 grams of sucrose, 4 grams of peptones, 2 grams
Yeast extract, 1 gram of K2HPO4, 1 gram of KH2PO4With 1 gram of MgSO4, stirring and dissolving is settled to 1000 milliliters with tap water；It is separated into bottle
It is put into sterilizing in autoclave sterilizer, it is cooling, it is spare at liquid spawn culture medium.
2. preparing meat cordyceps sinensis solid medium
New fresh mutton is cleaned, peeling is boned, chopping；One times of tap water is added, adds 1% sugar and 0.5% salt,
It is uniformly mixed, crushes；It is packed into strips in heat safe culture vessel after crushing, the meat mincing height 3mm of loading, in addition there is stomata
Lid, will culture vessel be packed into sterilizer in；It is carried out disinfection with high steam, then 0.15MPa 30 minutes blocks steam, waits for
When pressure is down to zero naturally, sterilizer is opened, takes out culture vessel, cooled to room temperature, is prepared by meat cordyceps sinensis solid medium
It completes.Meat cordyceps sinensis solid medium need to complete inoculation in 3 hours.
3. being inoculated on meat cordyceps sinensis solid medium
In transfer room, production cordyceps sinensis liquid spawn is diluted 8 times with the water to sterilize in the watering can sterilized,
Then it uniformly sows grass seeds by duster respectively on the meat cordyceps sinensis solid medium prepared, that is, completes inoculation；
4. meat cordyceps sinensis is cultivated
The culture vessel for being inoculated with liquid spawn is placed on culturing rack, by cordyceps sinensis be factory produced conventional method into
Row culture；Cultural hypha phase, humidity 70%~80%, 22 ± 1 DEG C of temperature take the measure of being protected from light；Wait for that mycelia is covered with solid culture
When base, into fructification culture, the control of fructification cultivation stage condition：Humidity 70%~80%, 25 ± 1 DEG C of temperature, when illumination
Between 12h illumination 12h or dark alternately, using fluorescent lamp lighting, intensity of illumination 1500lux；
5. meat cordyceps sinensis harvests
Meat cordyceps militaris sporocarp is grown to 5~8 centimeters, when occurring molecule in fructification, is harvested, by what is connected together
Meat cordyceps sinensis and meat culture medium of Cordyceps militaris are integrally that meat cordyceps sinensis herb is dug out from culture vessel together, are placed.A part first harvests meat
Cordyceps militaris sporocarp, then harvest meat culture medium of Cordyceps militaris；
6. the edible medicinal of meat cordyceps sinensis
The mutton meat cordyceps sinensis of harvesting or mutton meat cordyceps militaris sporocarp or meat culture medium of Cordyceps militaris, can eat raw；It can also be cold
It eats behind Tibetan, is eaten after drying can also be harvested, it can also crushed after being dried comprehensive utilization.Mutton meat cordyceps sinensis has a strand mutton taste.
Mutton meat cordyceps sinensis is eaten raw immediately, and fresh and tender deliciousness is completely different with the taste for cordyceps sinensis of being a vegetarian.
Embodiment 3
A kind of method of artificial culture meat cordyceps sinensis：
1. preparing the production of meat cordyceps sinensis uses liquid spawn
With embodiment 1
2. preparing meat cordyceps sinensis solid medium
New fresh chicken meat is cleaned, is boned；One times of tap water is added, adds 1% sugar and 0.5% salt, is uniformly mixed,
It crushes；It is packed into strips in heat safe culture vessel after crushing, the meat mincing height 2mm of loading, in addition leachy lid, will train
Container is supported to be packed into sterilizer；It is carried out disinfection with high steam, then 0.15MPa 30 minutes blocks steam, wait for pressure nature
When being down to zero, sterilizer is opened, takes out culture vessel, cooled to room temperature, prepared by meat cordyceps sinensis solid medium completes.Meat worm
Careless solid medium need to complete inoculation in 3 hours.
3. being inoculated on meat cordyceps sinensis solid medium
With embodiment 1
4. meat cordyceps sinensis is cultivated
With embodiment 1
5. the harvesting of meat cordyceps sinensis
With embodiment 1
6, the edible medicinal of meat cordyceps sinensis
With embodiment 1
The present invention uses the method that livestock meat produces meat cordyceps sinensis as solid medium for the first time, it is proposed that meat cordyceps sinensis eats
The concept of meat or fish cordyceps sinensis.The meat cordyceps militaris sporocarp and solid meat culture medium turned out using this method are integrally unified, edible and medicine
It is suitable with wild cordyceps with being worth, for the comprehensive exploitation of cordyceps sinensis, factorial praluction, medicinal, edible and further activity
Constituent analysis detaches with extraction and opens wide new foreground.
The meat cordyceps militaris sporocarp fresh goods of the present invention can directly be eaten, fresh and tender delicious；The present invention's makes meat culture medium of Cordyceps militaris
It is fully used.The product of various forms can be made in meat cordyceps sinensis dry product according to the market demand, can both be made effectively at
Divide readily soluble granule, can also handle and capsule is made, convenient for commercial sales and storage.
Simple production process of the present invention, at low cost cheap, raw material sources are extensive, pollution-free, it can be achieved that large-scale industrialized
Production provides a kind of new resources that the health substituting wild Chinese caterpillar fungus is good medicinal and edible for masses.
Claims (6)
1. a kind of method of artificial culture meat cordyceps sinensis, which is characterized in that using livestock meat as the solid culture of artificial culture cordyceps sinensis
Base produces the meat cordyceps sinensis that meat cordyceps militaris sporocarp and meat culture medium of Cordyceps militaris are integrally unified.
2. the method for artificial culture meat cordyceps sinensis as described in claim 1, which is characterized in that include the following steps：
Step 1)：The meat cordyceps sinensis production preparation of liquid spawn：
Cordyceps sinensis wild-type strain is acquired, routine culture acquisition meat cordyceps sinensis production is carried out to it and uses liquid spawn, it is spare；
Step 2)：The preparation of meat cordyceps sinensis solid medium：
It by fresh livestock meat, cleans, peeling is boned, chopping, and sugar is added and salt is uniformly mixed, crushes, is filled immediately after crushing
Enter and carry out steam high-temperature sterilization in culture vessel, high-temperature sterilization postcooling to room temperature is made meat cordyceps sinensis solid medium, is transferred to
Transfer room is for use；
Step 3)：It is inoculated on meat cordyceps sinensis solid medium：
Meat cordyceps sinensis is produced and is inoculated on meat cordyceps sinensis solid medium with liquid spawn with the mode of sowing grass seeds by duster；
Step 4)：The culture of meat cordyceps sinensis：
Culture vessel is placed on culturing rack, the conventional method culture meat cordyceps sinensis being factory produced by cordyceps sinensis；
Step 5)：The harvesting of meat cordyceps sinensis：
When there is molecule on the meat cordyceps militaris sporocarp on meat culture medium of Cordyceps militaris, show that meat cordyceps sinensis enters the stage of ripeness, this
When it is harvested.
3. the method for artificial culture meat cordyceps sinensis as claimed in claim 2, which is characterized in that the cordyceps sinensis in the step 1) is wild
Strain is aweto strain or Cordyceps militaris spawn.
4. the method for artificial culture meat cordyceps sinensis as claimed in claim 2, which is characterized in that the fresh livestock and poultry in the step 2)
Meat individually uses, or the sugar of meat quality 1% is added and 0.5% salt crushes together with meat.
5. the method for artificial culture meat cordyceps sinensis as claimed in claim 2, which is characterized in that the harvesting in the step 5) is specific
For：By the meat cordyceps militaris sporocarp to connect together and meat culture medium of Cordyceps militaris, integrally i.e. meat cordyceps sinensis harvests together, or first harvests meat worm
Grass seed entity, then harvest meat culture medium of Cordyceps militaris.
6. the method for artificial culture meat cordyceps sinensis as claimed in claim 2, which is characterized in that the meat cordyceps sinensis of the step 5) harvesting
Edible medicinal method be：It is eaten after fresh food, refrigeration, dry product or crushed after being dried comprehensive utilization is made.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112314330A (en) * | 2020-11-13 | 2021-02-05 | 浙江泛亚生物医药股份有限公司 | Method for culturing Roberts' cordyceps sinensis |
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