CN108934271A - A kind of method for treating seeds improving tea tree winter resistance - Google Patents
A kind of method for treating seeds improving tea tree winter resistance Download PDFInfo
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- CN108934271A CN108934271A CN201810853444.8A CN201810853444A CN108934271A CN 108934271 A CN108934271 A CN 108934271A CN 201810853444 A CN201810853444 A CN 201810853444A CN 108934271 A CN108934271 A CN 108934271A
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- tea tree
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- polysaccharide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
Abstract
The invention belongs to tea tree planting technical fields, especially a kind of method for treating seeds for improving tea tree winter resistance, the specific method is as follows: (1) being immersed in copper chloride solution heat-insulation soaking and be passed through ultrasonic wave the tea tree seed of full grains and be ultrasonically treated, drip liquid is pulled out, is used60Co- gamma-rays is irradiated processing, must pre-process tea tree seed；(2) pretreatment tea tree seed is immersed in heat-insulation soaking in seed-soaking liquid, drip liquid is pulled out, magnetization treatment, cold plasma processing；The seed-soaking liquid, is prepared from the following raw materials: urea, citric acid Chelated Manganese, astragalus polyose, Phellinus polysaccharide, dewdrop grass polysaccharide, Polysaccharide from Portulaca oleracea, water；The vitality and enzymatic activity of tea tree seed can effectively be enhanced, enhance Nutrition and Metabolism ability, the strong bud of hair is promoted to go out strong sprout; it effectively prevent Lipid peroxidation metabolism under tea tree low temperature environment; the stability for increasing cell membrane, effectively improves the cold performance of tea tree, protects tea seedling from the damage of low temperature.
Description
Technical field
The invention belongs to tea tree planting technical field, especially a kind of method for treating seeds for improving tea tree winter resistance.
Background technique
Tea tree, shrub or dungarunga, spray is hairless, leaf keratin, and oblong or ellipse, apex is blunt or sharp, base portion wedge
Shape, above it is shinny, below it is hairless or have pubescence at the beginning, there is sawtooth at edge, and petiole is hairless, grey color, and anthocaulus is sometimes slightly long, sepal
It is wealthy oval to round, it is hairless, it harbors, petal is wealthy oval, and base portion omits joint, and the back side is hairless, there is pubescence sometimes, and ovary is dense white
Hair, style is hairless, and capsule 3 is spherical or 1 ~ 2 is spherical, and 1.1 ~ 1.5 centimetres high, every ball has 1 ~ 2, seed.October at florescence to next year 2
Month；Tea tree property likes warm and moist weather, and bud starts to sprout at 10 DEG C of temperature on average or more, and growth optimum temperature is 20 ~ 25 DEG C, year
Precipitation will be at 1000 millimeters or more；Light-loving and shade-tolerating, suitable for being given birth under diffused light；Temperature is too low tea tree can be made by freeze injury and
Damage, so that tea bush productivity and quality substantially reduce under low temperature environment, even results in the death of tea tree, gives Tea planting family
Economic well-being of workers and staff bring very big adverse effect, so should invent it is a kind of improve tea tree winter resistance processing method.
Summary of the invention
In view of the above-mentioned problems, the present invention is intended to provide a kind of method for treating seeds for improving tea tree winter resistance.
The invention is realized by the following technical scheme:
A kind of method for treating seeds improving tea tree winter resistance, the specific method is as follows:
(1) by the tea tree seed of full grains be immersed in temperature be heat-insulation soaking 33 in 44 ~ 46 DEG C of copper chloride solution ~
37min is simultaneously passed through ultrasonic wave and is ultrasonically treated, and drip liquid is pulled out, the use of exposure dose is 5.7 ~ 6.1Gy's60Co- gamma-rays into
Row treatment with irradiation must pre-process tea tree seed；
(2) tea tree seed will be pre-processed and be immersed in 68 ~ 76min of heat-insulation soaking in the seed-soaking liquid that temperature is 39 ~ 41 DEG C, drip liquid fishing
Out, 420 ~ 450s of magnetization treatment carries out cold plasma under conditions of power is 85 ~ 89W and handles 18 ~ 20min；
The seed-soaking liquid, is made of raw material from the following weight: 2.2 ~ 2.4 parts of urea, 1.1 ~ 1.3 parts of citric acid Chelated Manganese,
0.15 ~ 0.17 part of astragalus polyose, 0.11 ~ 0.13 part of Phellinus polysaccharide, 0.20 ~ 0.22 part of dewdrop grass polysaccharide, Polysaccharide from Portulaca oleracea 0.25 ~
0.27 part, 660 ~ 680 parts of water.
As invention further embodiment: copper chloride solution described in step (1) is prepared according to the following steps: will
The copper chloride of 1.7 ~ 1.9 parts by weight and the radix paeoniae rubra polysaccharide of 0.13 ~ 0.15 parts by weight put into the water of 610 ~ 630 parts by weight stir to
Dissolution, obtains copper chloride solution.
As invention further embodiment: ultrasonic treatment described in step (1) is 147 ~ 153W, frequency 101 in power
9 ~ 11min is ultrasonically treated under conditions of ~ 105kHz, it is ultrasonic under conditions of power is 241 ~ 249W, frequency is 164 ~ 170kHz
Handle 24 ~ 26min.
As invention further embodiment: magnetization treatment described in step (2), in the condition that magnetic field strength is 430 ~ 450G
70 ~ 80s of lower magnetization treatment, 190 ~ 200s of magnetization treatment under conditions of magnetic field strength is 900 ~ 920G are in magnetic field strength
160 ~ 170s of magnetization treatment under conditions of 1600 ~ 1620G.
Beneficial effects of the present invention: a kind of method for treating seeds improving tea tree winter resistance provided by the invention, it can be effective
Enhance the vitality and enzymatic activity of tea tree seed, enhance Nutrition and Metabolism ability, promotes the strong bud of hair to go out strong sprout, moreover it is possible to effectively facilitate
The accumulation of the osmotic adjustments such as tea seedling proline, soluble sugar, soluble protein, not only improves tea tree under low temperature environment
The water holding capacity of seedling cell, protection biomembrane and cell life substance prevent active oxygen to the oxidation of film rouge and memebrane protein,
Nutrition and energy matter are supplied for the growth under the environment of status, dehydration is effectively prevent, enhances the aquation of protein, is protected
The stabilization of structure of biological macromolecule and function, the activity of excitation superoxide dismutase, catalase and polyphenol oxidase, mentions
The concentration of high cell liquid increases cell moisture holding capacity, and cytoplasm excessive dehydration is effectively relieved, and protection cytoplasm colloid will not be met
Cooling solidification effectively prevent Lipid peroxidation metabolism under tea tree low temperature environment, increases the stability of cell membrane, effectively improves tea tree
Cold performance protects tea seedling from the damage of low temperature.
Specific embodiment
Illustrate the present invention with specific embodiment below, but is not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of method for treating seeds improving tea tree winter resistance, the specific method is as follows:
(1) the tea tree seed of full grains is immersed in heat-insulation soaking 33min in the copper chloride solution that temperature is 44 DEG C and led to
Enter ultrasonic wave to be ultrasonically treated, drip liquid is pulled out, the use of exposure dose is 5.7Gy's60Co- gamma-rays is irradiated processing, obtains
Pre-process tea tree seed；
(2) tea tree seed will be pre-processed and is immersed in 68 ~ min of heat-insulation soaking in the seed-soaking liquid that temperature is 39 DEG C, drip liquid is pulled out, magnetized
420s is handled, cold plasma is carried out under conditions of power is 85W and handles 18min；
The seed-soaking liquid, is made of raw material from the following weight: 2.2 parts of urea, 1.1 parts of citric acid Chelated Manganese, astragalus polyose
0.15 part, 0.11 part of Phellinus polysaccharide, 0.20 part of dewdrop grass polysaccharide, 0.25 part of Polysaccharide from Portulaca oleracea, 660 parts of water.
As invention further embodiment: copper chloride solution described in step (1) is prepared according to the following steps: will
The copper chloride of 1.7 parts by weight and the radix paeoniae rubra polysaccharide of 0.13 parts by weight put into stirring in the water of 610 parts by weight and obtain copper chloride to dissolving
Aqueous solution.
As invention further embodiment: ultrasonic treatment described in step (1), power be 147W, frequency 101kHz
Under conditions of be ultrasonically treated 9min, under conditions of power is 241W, frequency is 164kHz be ultrasonically treated 24 ~ 26min.
As invention further embodiment: magnetization treatment described in step (2), magnetic under conditions of magnetic field strength is 430G
Change processing 70s, magnetization treatment 190s under conditions of magnetic field strength is 900G magnetizes under conditions of magnetic field strength is 1600G
Handle 160s.
Embodiment 2
In the embodiment of the present invention, a kind of method for treating seeds improving tea tree winter resistance, the specific method is as follows:
(1) the tea tree seed of full grains is immersed in heat-insulation soaking 35min in the copper chloride solution that temperature is 45 DEG C and led to
Enter ultrasonic wave to be ultrasonically treated, drip liquid is pulled out, the use of exposure dose is 5.9Gy's60Co- gamma-rays is irradiated processing, obtains
Pre-process tea tree seed；
(2) tea tree seed will be pre-processed and is immersed in heat-insulation soaking 72min in the seed-soaking liquid that temperature is 40 DEG C, drip liquid is pulled out, magnetized
435s is handled, cold plasma is carried out under conditions of power is 87W and handles 19min；
The seed-soaking liquid, is made of raw material from the following weight: 2.3 parts of urea, 1.2 parts of citric acid Chelated Manganese, astragalus polyose
0.16 part, 0.12 part of Phellinus polysaccharide, 0.21 part of dewdrop grass polysaccharide, 0.26 part of Polysaccharide from Portulaca oleracea, 670 parts of water.
As invention further embodiment: copper chloride solution described in step (1) is prepared according to the following steps: will
The copper chloride of 1.8 parts by weight and the radix paeoniae rubra polysaccharide of 0.14 parts by weight put into stirring in the water of 620 parts by weight and obtain copper chloride to dissolving
Aqueous solution.
As invention further embodiment: ultrasonic treatment described in step (1), power be 150W, frequency 103kHz
Under conditions of be ultrasonically treated 10min, be ultrasonically treated 25min under conditions of power is 245W, frequency is 167kHz.
As invention further embodiment: magnetization treatment described in step (2), magnetic under conditions of magnetic field strength is 440G
Change processing 75s, magnetization treatment 195s under conditions of magnetic field strength is 910G magnetizes under conditions of magnetic field strength is 1610G
Handle 165s.
Embodiment 3
In the embodiment of the present invention, a kind of method for treating seeds improving tea tree winter resistance, the specific method is as follows:
(1) the tea tree seed of full grains is immersed in heat-insulation soaking 37min in the copper chloride solution that temperature is 46 DEG C and led to
Enter ultrasonic wave to be ultrasonically treated, drip liquid is pulled out, the use of exposure dose is 6.1Gy's60Co- gamma-rays is irradiated processing, obtains
Pre-process tea tree seed；
(2) tea tree seed will be pre-processed and is immersed in heat-insulation soaking 76min in the seed-soaking liquid that temperature is 41 DEG C, drip liquid is pulled out, magnetized
450s is handled, cold plasma is carried out under conditions of power is 89W and handles 20min；
The seed-soaking liquid, is made of raw material from the following weight: 2.4 parts of urea, 1.3 parts of citric acid Chelated Manganese, astragalus polyose
0.17 part, 0.13 part of Phellinus polysaccharide, 0.22 part of dewdrop grass polysaccharide, 0.27 part of Polysaccharide from Portulaca oleracea, 680 parts of water.
As invention further embodiment: copper chloride solution described in step (1) is prepared according to the following steps: will
The copper chloride of 1.9 parts by weight and the radix paeoniae rubra polysaccharide of 0.15 parts by weight put into stirring in the water of 630 parts by weight and obtain copper chloride to dissolving
Aqueous solution.
As invention further embodiment: ultrasonic treatment described in step (1), power be 153W, frequency 105kHz
Under conditions of be ultrasonically treated 11min, be ultrasonically treated 26min under conditions of power is 249W, frequency is 170kHz.
As invention further embodiment: magnetization treatment described in step (2), magnetic under conditions of magnetic field strength is 450G
Change processing 80s, magnetization treatment 200s under conditions of magnetic field strength is 920G, under conditions of magnetic field strength is 1600 ~ 1620G
Magnetization treatment 170s.
Comparative example 1
A kind of method for treating seeds improving tea tree winter resistance, the difference from embodiment 1 is that, by chlorination described in step (1)
Copper liquor is replaced with clear water, and other conditions are all the same.
Comparative example 2
It is a kind of improve tea tree winter resistance method for treating seeds, the difference from embodiment 1 is that, step (1) without ultrasound at
Reason, other conditions are all the same.
Comparative example 3
It is a kind of improve tea tree winter resistance method for treating seeds, the difference from embodiment 1 is that, step (1) without60Co-γ
Radiation exposure processing, other conditions are all the same.
Comparative example 4
A kind of method for treating seeds improving tea tree winter resistance, the difference from embodiment 1 is that, by seed soaking described in step (2)
Liquid is replaced with clear water, and other conditions are all the same.
Comparative example 5
It is a kind of improve tea tree winter resistance method for treating seeds, the difference from embodiment 1 is that, step (2) without magnetization at
Reason, other conditions are all the same.
Comparative example 6
A kind of method for treating seeds improving tea tree winter resistance, the difference from embodiment 1 is that, step (2) without it is cold it is equal from
Daughter processing, other conditions are all the same.
Comparative example 7
The processing method of the tea tree seed of the prior art impregnates 120min using the Gibberellins solution that mass fraction is 100ppm.
" Anhui 3 " the tea tree seed of full grains is selected to use the processing method of embodiment and the processing method of comparative example
It is handled, sowed after processing (it is seeded in the plastic cup for installing soil, every glass is broadcast 1, and it sets in glasshouse and cultivates,
Diurnal temperature is 19 DEG C/15 DEG C, and PDF is 120 μm of ol/(ms), RH 75%), in addition to method for treating seeds is different, other
Part is all the same, and low temperature stress processing is carried out when cultivating to 31 heart of leaf (will coerce in growth cabinet that seedling moves to -4 ± 1 DEG C
20d, PDF are 50 μm of ol/(ms), RH 55%, photoperiod 12h/12h), low temperature stress after treatment measures reality respectively
Applying example winter resistance index related to comparative example tea tree seedling, (measurement of proline content uses ninhydrin method；Superoxides discrimination
Change the method that Zhu Guanglian is pressed in enzyme assay；The measurement of mda content uses thiobarbituricacidα- colorimetric method), as a result such as table 1:
The comparing result of 1 embodiment and comparative example of table
As it can be seen from table 1 the tea tree seed obtained after processing method processing of the invention is cultivated, winter resistance is obviously high
In the tea seedling that the processing method of the tea tree seed of 7 prior art of comparative example is cultivated, there is high winter resistance.
Claims (4)
1. a kind of method for treating seeds for improving tea tree winter resistance, which is characterized in that the specific method is as follows:
(1) by the tea tree seed of full grains be immersed in temperature be heat-insulation soaking 33 in 44 ~ 46 DEG C of copper chloride solution ~
37min is simultaneously passed through ultrasonic wave and is ultrasonically treated, and drip liquid is pulled out, the use of exposure dose is 5.7 ~ 6.1Gy's60Co- gamma-rays into
Row treatment with irradiation must pre-process tea tree seed；
(2) tea tree seed will be pre-processed and be immersed in 68 ~ 76min of heat-insulation soaking in the seed-soaking liquid that temperature is 39 ~ 41 DEG C, drip liquid fishing
Out, 420 ~ 450s of magnetization treatment carries out cold plasma under conditions of power is 85 ~ 89W and handles 18 ~ 20min；
The seed-soaking liquid, is made of raw material from the following weight: 2.2 ~ 2.4 parts of urea, 1.1 ~ 1.3 parts of citric acid Chelated Manganese,
0.15 ~ 0.17 part of astragalus polyose, 0.11 ~ 0.13 part of Phellinus polysaccharide, 0.20 ~ 0.22 part of dewdrop grass polysaccharide, Polysaccharide from Portulaca oleracea 0.25 ~
0.27 part, 660 ~ 680 parts of water.
2. the method for treating seeds according to claim 1 for improving tea tree winter resistance, which is characterized in that step (1) is described
Copper chloride solution, prepared according to the following steps: by the copper chloride of 1.7 ~ 1.9 parts by weight and 0.13 ~ 0.15 parts by weight
Radix paeoniae rubra polysaccharide puts into stirring in the water of 610 ~ 630 parts by weight and obtains copper chloride solution to dissolving.
3. the method for treating seeds according to claim 1 for improving tea tree winter resistance, which is characterized in that step (1) is described
Ultrasonic treatment, under conditions of power is 147 ~ 153W, frequency is 101 ~ 105kHz be ultrasonically treated 9 ~ 11min, be in power
241 ~ 249W, frequency are ultrasonically treated 24 ~ 26min under conditions of being 164 ~ 170kHz.
4. the method for treating seeds according to claim 1 for improving tea tree winter resistance, which is characterized in that step (2) is described
Magnetization treatment, magnetization treatment 70 ~ 80s under conditions of magnetic field strength is 430 ~ 450G is 900 ~ 920G in magnetic field strength
Under the conditions of 190 ~ 200s of magnetization treatment, magnetization treatment 160 ~ 170s under conditions of magnetic field strength is 1600 ~ 1620G.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810853444.8A CN108934271A (en) | 2018-07-30 | 2018-07-30 | A kind of method for treating seeds improving tea tree winter resistance |
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| CN201810853444.8A CN108934271A (en) | 2018-07-30 | 2018-07-30 | A kind of method for treating seeds improving tea tree winter resistance |
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| CN108934271A true CN108934271A (en) | 2018-12-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112567990A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Cutting seedling method for improving survival rate of tea trees in alpine mountain areas |
| CN112567989A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Ecological planting method for improving quality of tea in alpine mountain area |
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| CN102498873A (en) * | 2011-11-01 | 2012-06-20 | 青岛农业大学 | Identification method of Tea plant germ plasma resource seedling-stage coldness resistance |
| CN108718590A (en) * | 2018-04-11 | 2018-11-02 | 霍山县庆中农林开发有限公司 | A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance |
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Patent Citations (2)
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| CN102498873A (en) * | 2011-11-01 | 2012-06-20 | 青岛农业大学 | Identification method of Tea plant germ plasma resource seedling-stage coldness resistance |
| CN108718590A (en) * | 2018-04-11 | 2018-11-02 | 霍山县庆中农林开发有限公司 | A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112567990A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Cutting seedling method for improving survival rate of tea trees in alpine mountain areas |
| CN112567989A (en) * | 2020-11-28 | 2021-03-30 | 巢湖市年晟农业生态有限公司 | Ecological planting method for improving quality of tea in alpine mountain area |
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Application publication date: 20181207 |