CN108718590A - A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance - Google Patents

A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance Download PDF

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Publication number
CN108718590A
CN108718590A CN201810321374.1A CN201810321374A CN108718590A CN 108718590 A CN108718590 A CN 108718590A CN 201810321374 A CN201810321374 A CN 201810321374A CN 108718590 A CN108718590 A CN 108718590A
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seed
parts
camellia oleifera
electric
processing
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李庆中
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Mount Holyoke County Qing Zhong Agriculture And Forestry Development Co Ltd
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Mount Holyoke County Qing Zhong Agriculture And Forestry Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

Abstract

The invention belongs to seed biologic treating technique field, especially a kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, the specific method is as follows:(1)Seed of Camellia oleifera is placed in electric field and carries out electric field treatment, is immersed in seed soaking agent and is impregnated, stirred with blender, filtering agent pulls Seed of Camellia oleifera out;The seed soaking agent is made of following raw material:Isoalantolactone, polyethylene glycol, water;(2)It is placed in household freezer and carries out freezing processing, take out, constant temperature frying, take out, be immersed in nutritional agents and impregnated, filtering agent pulls Seed of Camellia oleifera, microwave drying out;The nutritional agents is made of following raw material:Fructus piperis longi polysaccharide, antierythrite, robiniae,flos juice, kudzu root flavone, calgon, ferrous sulfate, water;Oil tea germination percentage is not only effectively improved, survival rate of seedling is improved, shortens growing-seedling period, effectively reduces seedling cost, and effectively facilitate the soluble sugar, soluble protein and Proline Accumulation of oil tea under low temperature, improves cold tolerance.

Description

A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance
Technical field
The invention belongs to seed biologic treating technique field, especially a kind of raising Seed of Camellia oleifera Emerging ability and cold-resistant energy The processing method of power.
Background technology
One of main nonwood forest trees in oil tea China, happiness is warm, is afraid of cold, is most productive value in Camellia Plants Oil with seeds, with oil palm, olive and coconut and referred to as four big woody edible oil tannin plant of the world;Low temperature stress is plant cultivation A kind of disaster frequently encountered in training, southern frost in 2008 just makes many industrial crops by huge loss, although planting Object itself has certain winter resistance, but different floristics and kind have different winter resistances, the winter resistance of Different Organs Also different;Therefore, the physiological Mechanism for exploring plant cold resistance is not only of great significance in basic theory, but also for people Instructing manufacture, rationally introduce a fine variety, breeding and cultivation management, it is significant to reduce natural calamity;Oil tea is adaptable, resistance to lean It is thin, and ground is not striven with grain, and be ideal water and soil conservation, antipollution and fire prevention isolation seeds, tea oil is full of nutrition, economic value Height, oil tea cultivation is increasingly by social concerns, but temperature is to influence Growth of Camellia oleifera development, limits the master of oil tea geographical distribution Environmental factor is wanted, when oil tea autumn and winter blooms, low temperature easily causes poor pollination or petal to fall off in advance, frostbite young fruit, causes to produce Amount declines.
Invention content
In view of the above-mentioned problems, the present invention is intended to provide a kind of processing side for improving Seed of Camellia oleifera Emerging ability and cold tolerance Method.
The invention is realized by the following technical scheme:
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, the specific method is as follows:
(1)By full grains and without small holes caused by worms Seed of Camellia oleifera merging electric field in carry out electric field treatment, be immersed in seed soaking agent and carry out It impregnates, with the blender that rotating speed is 170 ~ 190r/min, 23 ~ 27min of stirring, adjustment agitator speed are 82 ~ 88r/ clockwise 44 ~ 48min of stirring, filtering agent pull Seed of Camellia oleifera out to min counterclockwise;
The seed soaking agent is made of the raw material of following parts by weight:1.2 ~ 1.4 parts of isoalantolactone, polyethylene glycol 16 ~ 18 Part, 130 ~ 140 parts of water;
(2)It will be through step(1)Freezing processing is carried out in treated Seed of Camellia oleifera merging household freezer, is taken out, merging temperature is 51 ~ 14 ~ 18min of constant temperature frying in 55 DEG C of rotary frying pan takes out, is immersed in nutritional agents and is impregnated, filtering agent pulls oil tea kind out Son, microwave drying to moisture content is 13% ~ 15% under conditions of power is 131 ~ 139W;
The nutritional agents is made of the raw material of following parts by weight:0.66 ~ 0.78 part of fructus piperis longi polysaccharide, antierythrite 2.4 ~ 2.8 Part, 16 ~ 18 parts of robiniae,flos juice, 0.42 ~ 0.46 part of kudzu root flavone, 1.3 ~ 1.7 parts of calgon, 2.2 ~ 2.4 parts of ferrous sulfate, 170 ~ 190 parts of water.
As invention further embodiment:Step(1)The electric field treatment is 88 ~ 94kV/m, electric field in electric field strength Frequency handles 190 ~ 230s under conditions of being 11.2 ~ 12.4kHz, is 131 ~ 139kV/m, electric field frequency 15.1 in electric field strength 260 ~ 280s is handled under conditions of ~ 16.3kHz, in the condition that electric field strength is 64 ~ 68kV/m, electric field frequency is 8.8 ~ 9.4kHz 340 ~ 380s of lower processing.
As invention further embodiment:Step(2)The freezing processing, the item for being -19.8 ~ -18.6 DEG C in temperature Cryostat handles 8 ~ 10min under part, is warming up to -3.7 ~ -3.1 DEG C and continues cryostat 24 ~ 28min of processing, is cooled to -11.5 ~ -10.7 DEG C are continued cryostat and handle 34 ~ 38min.
As invention further embodiment:Step(2)The robiniae,flos juice, is prepared according to the following steps:By 24 ~ 28 The fresh robiniae,flos of parts by weight crush, and the trichoderma harzianum that 0.32 ~ 0.38 parts by weight are added is mixed evenly, and merging temperature is 34 ~ 40h of ferment at constant temperature in 29 ~ 31 DEG C of fermenting case takes out, and 34 ~ 38 parts by weight is added, the ethyl alcohol that mass fraction is 46% ~ 50% Solution is mixed evenly, and 52 ~ 58h of ferment at constant temperature in the fermenting case that merging temperature is 35 ~ 35 DEG C takes out, and filtering takes filtrate, Obtain robiniae,flos juice.
Beneficial effects of the present invention:A kind of processing improving Seed of Camellia oleifera Emerging ability and cold tolerance provided by the invention Method has been not only able to softening oil tea kind skin, and effect improves its oily permeable breathable, and effective breaking dormancy is supplied for Seed of Camellia oleifera Nutrition and energy effectively enhance the sprouting ability of Seed of Camellia oleifera, improve its germination percentage, improve survival rate of seedling, shorten nursery week Phase effectively reduces seedling cost, and can effectively facilitate soluble sugar, soluble protein and the proline product of oil tea under low temperature Tired, proline is a kind of water-soluble and relatively high osmotic adjustment of the flow of water, and plasm can be kept to be balanced with environment seepage, is protected The integrality for holding membrane structure, being combined with protein enhances its aquation, protects the stabilization of the structure and function of large biological molecule, When oil tea is by low temperature stress gesture, proline provides enough Free waters and life by the space structure of protective enzyme for biochemistry reflection Active material is managed, to be played a certain protective role to cell, soluble protein has osmotic adjustment, water conservation and reduces freezing point Effect, soluble sugar can improve cell liquid concentration, reduce freezing point, mitigate cytoplasm excessive dehydration, keep cell that will not be pre-chilled Solidification, to improve plant cold resistance, meanwhile, soluble sugar is also used as protective agent, to mitochondria and chloroplast membrane-bound coupling factor There is protective effect, to effectively improve the cold tolerance of oil tea, so that it is no longer persecuted by low temperature in growth course, low It also can normal robust growth under warm environment.
Specific implementation mode
Illustrate the present invention with specific embodiment below, but is not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, the specific method is as follows:
(1)By full grains and without small holes caused by worms Seed of Camellia oleifera merging electric field in carry out electric field treatment, be immersed in seed soaking agent and carry out It impregnates, the blender for being 170r/min with rotating speed stirs 23min clockwise, adjustment agitator speed is that 82r/min is stirred counterclockwise 44min is mixed, filtering agent pulls Seed of Camellia oleifera out;
The seed soaking agent is made of the raw material of following parts by weight:1.2 parts of isoalantolactone, 16 parts of polyethylene glycol, water 130 Part;
(2)It will be through step(1)The interior progress freezing processing of Seed of Camellia oleifera merging household freezer that treated, takes out, merging temperature is 51 DEG C rotary frying pan in constant temperature frying 14min, take out, be immersed in nutritional agents and impregnated, filtering agent pulls Seed of Camellia oleifera out, in work( Microwave drying to moisture content is 13% ~ 15% under conditions of rate is 131W;
The nutritional agents is made of the raw material of following parts by weight:0.66 part of fructus piperis longi polysaccharide, 2.4 parts of antierythrite, robiniae,flos juice 16 parts, 0.42 part of kudzu root flavone, 1.3 parts of calgon, 2.2 parts of ferrous sulfate, 170 parts of water.
As invention further embodiment:Step(1)The electric field treatment, electric field strength be 88kV/m, electric field frequency Rate handles 190s under conditions of being 11.2kHz, is handled under conditions of electric field strength is 131kV/m, electric field frequency is 15.1kHz 260s handles 340s under conditions of electric field strength is 64kV/m, electric field frequency is 8.8kHz.
As invention further embodiment:Step(2)The freezing processing, it is permanent under conditions of temperature is -19.8 DEG C Warm freezing processing 8min is warming up to -3.7 DEG C and continues cryostat processing 24min, is cooled to -11.5 DEG C and continues at cryostat Manage 34 ~ 38min.
As invention further embodiment:Step(2)The robiniae,flos juice, is prepared according to the following steps:By 24 weights The fresh robiniae,flos for measuring part crush, and the trichoderma harzianum that 0.32 parts by weight are added is mixed evenly, and merging temperature is 29 DEG C Ferment at constant temperature 34h in fermenting case takes out, and 34 parts by weight, mass fraction is added and is mixed evenly for 46% ethanol solution, sets Enter ferment at constant temperature 52h in the fermenting case that temperature is 35 DEG C, take out, filtering takes filtrate, obtains robiniae,flos juice.
Embodiment 2
In the embodiment of the present invention, a kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, the specific method is as follows:
(1)By full grains and without small holes caused by worms Seed of Camellia oleifera merging electric field in carry out electric field treatment, be immersed in seed soaking agent and carry out It impregnates, the blender for being 180r/min with rotating speed stirs 25min clockwise, adjustment agitator speed is that 85r/min is stirred counterclockwise 46min is mixed, filtering agent pulls Seed of Camellia oleifera out;
The seed soaking agent is made of the raw material of following parts by weight:1.3 parts of isoalantolactone, 17 parts of polyethylene glycol, water 135 Part;
(2)It will be through step(1)The interior progress freezing processing of Seed of Camellia oleifera merging household freezer that treated, takes out, merging temperature is 53 DEG C rotary frying pan in constant temperature frying 16min, take out, be immersed in nutritional agents and impregnated, filtering agent pulls Seed of Camellia oleifera out, in work( Microwave drying to moisture content is 13% ~ 15% under conditions of rate is 135W;
The nutritional agents is made of the raw material of following parts by weight:0.72 part of fructus piperis longi polysaccharide, 2.6 parts of antierythrite, robiniae,flos juice 17 parts, 0.44 part of kudzu root flavone, 1.5 parts of calgon, 2.3 parts of ferrous sulfate, 180 parts of water.
As invention further embodiment:Step(1)The electric field treatment, electric field strength be 91kV/m, electric field frequency Rate handles 210s under conditions of being 11.8kHz, is handled under conditions of electric field strength is 135kV/m, electric field frequency is 15.7kHz 270s handles 360s under conditions of electric field strength is 66kV/m, electric field frequency is 9.1kHz.
As invention further embodiment:Step(2)The freezing processing, it is permanent under conditions of temperature is -19.2 DEG C Warm freezing processing 9min is warming up to -3.4 DEG C and continues cryostat processing 26min, is cooled to -11.1 DEG C and continues at cryostat Manage 36min.
As invention further embodiment:Step(2)The robiniae,flos juice, is prepared according to the following steps:By 26 weights The fresh robiniae,flos for measuring part crush, and the trichoderma harzianum that 0.35 parts by weight are added is mixed evenly, and merging temperature is 30 DEG C Ferment at constant temperature 37h in fermenting case takes out, and 36 parts by weight, mass fraction is added and is mixed evenly for 48% ethanol solution, sets Enter ferment at constant temperature 55h in the fermenting case that temperature is 36 DEG C, take out, filtering takes filtrate, obtains robiniae,flos juice.
Embodiment 3
In the embodiment of the present invention, a kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, the specific method is as follows:
(1)By full grains and without small holes caused by worms Seed of Camellia oleifera merging electric field in carry out electric field treatment, be immersed in seed soaking agent and carry out It impregnates, the blender for being 190r/min with rotating speed stirs 27min clockwise, adjustment agitator speed is that 88r/min is stirred counterclockwise 48min is mixed, filtering agent pulls Seed of Camellia oleifera out;
The seed soaking agent is made of the raw material of following parts by weight:1.4 parts of isoalantolactone, 18 parts of polyethylene glycol, water 140 Part;
(2)It will be through step(1)The interior progress freezing processing of Seed of Camellia oleifera merging household freezer that treated, takes out, merging temperature is 55 DEG C rotary frying pan in constant temperature frying 18min, take out, be immersed in nutritional agents and impregnated, filtering agent pulls Seed of Camellia oleifera out, in work( Microwave drying to moisture content is 13% ~ 15% under conditions of rate is 139W;
The nutritional agents is made of the raw material of following parts by weight:0.78 part of fructus piperis longi polysaccharide, 2.8 parts of antierythrite, robiniae,flos juice 18 parts, 0.46 part of kudzu root flavone, 1.7 parts of calgon, 2.4 parts of ferrous sulfate, 190 parts of water.
As invention further embodiment:Step(1)The electric field treatment, electric field strength be 94kV/m, electric field frequency Rate handles 230s under conditions of being 12.4kHz, is handled under conditions of electric field strength is 139kV/m, electric field frequency is 16.3kHz 280s handles 380s under conditions of electric field strength is 68kV/m, electric field frequency is 9.4kHz.
As invention further embodiment:Step(2)The freezing processing, it is permanent under conditions of temperature is -18.6 DEG C Warm freezing processing 10min is warming up to -3.1 DEG C and continues cryostat processing 28min, is cooled to -10.7 DEG C and continues at cryostat Manage 38min.
As invention further embodiment:Step(2)The robiniae,flos juice, is prepared according to the following steps:By 28 weights The fresh robiniae,flos for measuring part crush, and the trichoderma harzianum that 0.38 parts by weight are added is mixed evenly, and merging temperature is 29 ~ 31 DEG C Fermenting case in ferment at constant temperature 40h, take out, be added 38 parts by weight, mass fraction be 50% ethanol solution be mixed evenly, It is placed in ferment at constant temperature 58h in the fermenting case that temperature is 37 DEG C, is taken out, filtering takes filtrate, obtains robiniae,flos juice.
Comparative example 1
The processing method of the Seed of Camellia oleifera of the prior art, as number of patent application is 201610693488.X, patent name is oil tea To the processing of Seed of Camellia oleifera in the method for nursery
Comparative example 2
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, difference lies in steps with embodiment 1(1) Without electric field treatment, other conditions all same.
Comparative example 3
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, difference lies in steps with embodiment 1(1) Isoalantolactone, other conditions all same are added without in the seed soaking agent.
Comparative example 4
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, difference lies in steps with embodiment 1(2) Without freezing processing, other conditions all same.
Comparative example 5
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, difference lies in steps with embodiment 1(2) Fructus piperis longi polysaccharide and antierythrite, other conditions all same are added without in the nutritional agents.
Comparative example 6
A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, difference lies in steps with embodiment 1(2) Robiniae,flos juice and kudzu root flavone, other conditions all same are added without in the nutritional agents.
The method of same a collection of No. 53 Seed of Camellia oleifera embodiment and comparative example is handled, it is real that nursery is carried out after processing It tests, the use of number of patent application is 201610693488.X, patent name except the different outer other conditions all sames of method for treating seeds Breeding experiment carried out to treated Seed of Camellia oleifera for the nursery processing in the method for camellia seedling, nursery to height of seedling be 15cm with On, its emergence rate, growing-seedling period and survival rate of seedling are recorded, as a result such as table 1, and per 50 plants of oil tea childrens of group selection after the end of nursery Seedling carries out -8 DEG C of low temperature stress processing(Handle 100h after, acquisition same area blade with distilled water flushing it is clean after be stored in it is super In low temperature refrigerator, it to be used for physiological index determining), detect its soluble content, soluble protein content and proline content(It is solvable Property sugar measurement using anthrone colorimetry measurement;The measurement of soluble protein uses Coomassie brilliant blue(G-250)Method;Proline Measurement using Li Hesheng assay method), as a result such as table 2:
The comparing result of 1 embodiment and comparative example of table
Emergence rate(%) Growing-seedling period(It) Survival rate of seedling(%)
Embodiment 1 100 20~21 99.8
Embodiment 2 99.3 21~22 99.2
Embodiment 3 99.6 21~22 99.4
Comparative example 1 85.4 30~31 82.3
Comparative example 2 88.2 27~28 84.6
Comparative example 3 88.7 27~28 84.4
Comparative example 4 89.8 27~28 85.7
Comparative example 5 84.1 26~27 81.9
Comparative example 6 84.9 26~27 81.3
As it can be seen from table 1 the processing method of the Seed of Camellia oleifera of the present invention can effectively improve the emergence rate of Seed of Camellia oleifera and educate Shoot survival percent shortens its growing-seedling period.
The cold tolerance comparing result of 2 embodiment and comparative example of table
Soluble sugar(mg/g) Soluble protein(mg/g) Proline(μg/g)
Embodiment 1 35.22 50.54 77.41
Embodiment 2 34.98 50.12 78.78
Embodiment 3 34.76 49.75 76.62
Comparative example 1 19.41 30.38 48.86
Comparative example 2 29.32 42.82 68.65
Comparative example 3 30.22 43.33 67.43
Comparative example 4 29.86 42.42 69.11
Comparative example 5 28.43 42.03 67.20
Comparative example 6 28.87 41.87 66.74
From table 2 it can be seen that the processing method of the Seed of Camellia oleifera of the present invention can be obviously improved the cold tolerance of oil tea.

Claims (5)

1. a kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance, which is characterized in that the specific method is as follows:
(1)By full grains and without small holes caused by worms Seed of Camellia oleifera merging electric field in carry out electric field treatment, be immersed in seed soaking agent and carry out It impregnates, with the blender that rotating speed is 170 ~ 190r/min, 23 ~ 27min of stirring, adjustment agitator speed are 82 ~ 88r/ clockwise 44 ~ 48min of stirring, filtering agent pull Seed of Camellia oleifera out to min counterclockwise;
The seed soaking agent is made of the raw material of following parts by weight:1.2 ~ 1.4 parts of isoalantolactone, polyethylene glycol 16 ~ 18 Part, 130 ~ 140 parts of water;
(2)It will be through step(1)Freezing processing is carried out in treated Seed of Camellia oleifera merging household freezer, is taken out, merging temperature is 51 ~ 14 ~ 18min of constant temperature frying in 55 DEG C of rotary frying pan takes out, is immersed in nutritional agents and is impregnated, filtering agent pulls oil tea kind out Son, microwave drying to moisture content is 13% ~ 15% under conditions of power is 131 ~ 139W;
The nutritional agents is made of the raw material of following parts by weight:0.66 ~ 0.78 part of fructus piperis longi polysaccharide, antierythrite 2.4 ~ 2.8 Part, 16 ~ 18 parts of robiniae,flos juice, 0.42 ~ 0.46 part of kudzu root flavone, 1.3 ~ 1.7 parts of calgon, 2.2 ~ 2.4 parts of ferrous sulfate, 170 ~ 190 parts of water.
2. the processing method according to claim 1 for improving Seed of Camellia oleifera Emerging ability and cold tolerance, which is characterized in that Step(1)The electric field treatment, under conditions of electric field strength is 88 ~ 94kV/m, electric field frequency is 11.2 ~ 12.4kHz at Manage 190 ~ 230s, under conditions of electric field strength be 131 ~ 139kV/m, electric field frequency is 15.1 ~ 16.3kHz processing 260 ~ 280s handles 340 ~ 380s under conditions of electric field strength is 64 ~ 68kV/m, electric field frequency is 8.8 ~ 9.4kHz.
3. the method for treating seeds according to claim 1 for improving Seed of Camellia oleifera germination percentage and cold tolerance, feature exist In step(2)The freezing processing, cryostat handles 8 ~ 10min under conditions of temperature is -19.8 ~ -18.6 DEG C, rises Temperature continues cryostat to -3.7 ~ -3.1 DEG C and handles 24 ~ 28min, is cooled to -11.5 ~ -10.7 DEG C and continues cryostat processing 34 ~38min。
4. the processing method according to claim 1 for improving Seed of Camellia oleifera Emerging ability and cold tolerance, which is characterized in that Step(2)The robiniae,flos juice, is prepared according to the following steps:The fresh robiniae,flos of 24 ~ 28 parts by weight are crushed, are added The trichoderma harzianum of 0.32 ~ 0.38 parts by weight is mixed evenly, ferment at constant temperature 34 in the fermenting case that merging temperature is 29 ~ 31 DEG C ~ 40h takes out, and 34 ~ 38 parts by weight, mass fraction is added and is mixed evenly for 46% ~ 50% ethanol solution, and merging temperature is 52 ~ 58h of ferment at constant temperature in 35 ~ 35 DEG C of fermenting case takes out, and filtering takes filtrate, obtains robiniae,flos juice.
CN201810321374.1A 2018-04-11 2018-04-11 A kind of processing method improving Seed of Camellia oleifera Emerging ability and cold tolerance Withdrawn CN108718590A (en)

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谭云峰: "《油茶高效栽培技术与加工》", 31 August 2016 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108934271A (en) * 2018-07-30 2018-12-07 霍山茗源茶树良种开发有限公司 A kind of method for treating seeds improving tea tree winter resistance

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