CN108934266A - A kind of method for treating seeds improving Peach crown gall disease incidence - Google Patents
A kind of method for treating seeds improving Peach crown gall disease incidence Download PDFInfo
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- CN108934266A CN108934266A CN201810585653.9A CN201810585653A CN108934266A CN 108934266 A CN108934266 A CN 108934266A CN 201810585653 A CN201810585653 A CN 201810585653A CN 108934266 A CN108934266 A CN 108934266A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Abstract
The invention discloses a kind of method for treating seeds for improving Peach crown gall disease incidence, belong to planting fruit trees technical field, comprising the following steps: (1) preparation of seed-soaking liquid；(2) seed soaking；(3) ultraviolet irradiation.The present invention handles peach seed, effectively improve the characteristic of peach seed, it can not only prevent Peach crown gall, also improve its germination percentage and disease-resistant rate, robust growth, and it is nontoxic to environment, or even certain positive effect is played to soil remediation, there is good market popularization value.
Description
Technical field
The invention belongs to planting fruit trees technical fields, and in particular to a kind of seed treatment for improving Peach crown gall disease incidence
Method.
Background technique
Peach is a kind of defoliation small arbor of fruit as fruit, and spending can watch, fruit succulence.The dry upper secretion of peach
Colloid is commonly called as peach gum, can be used as bonding agent etc., be a kind of glycan substance, hydrolysis can generate arabinose, galactolipin, xylose,
Rhamnose, glucuronic acid etc., edible, also hyoscine, there is the benefits of blood-breaking and blood, QI invigorating.Peach is known as " peach offered as a birthday present " and " flat peach "
Laudatory title, because of its delicious meat, be otherwise known as " peerless fruit ".Peach meat containing protein, fat, carbohydrate, crude fibre,
Calcium, phosphorus, iron, carrotene, vitamin B1 and organic acid, sugar and volatile oil are suitable for Diagnostic value and Patients with iron deficiency anemia
It is edible.
The disease is bacterial disease, and pathogenetic bacteria is Agrobacterium tumefaciens, and host range is very extensive.Pathogenetic bacteria is in root
It is overwintering in the cortex of tumor tissue, or when carcinoma ruptures and peels enter soil in it is overwintering, the several months that can survive in the soil was to 1 year
It is more.Rainwater, to pour water, move soil etc. be major transmission path, and subterranean pest-insect such as grub, mole cricket, nematode etc. also have certain propagation to make
With nursery stock is the main mode propagated over long distances in spite of illness.Bacterium encounters the wound of root system, such as insect bite, mechanical damage, graft union
Deng, cortical tissue is invaded, starts to breed, and stimulates wound cell division nearby, formation carcinoma.Basic soil is conducive to fall ill；
The glutinous weight of soil, the orchard morbidity of impeded drainage are more；The morbidity of cut-grafting nursery stock is more, and the morbidity of budling wood is less；Graft union is in soil
Be conducive to fall ill below face, fall ill more than native face relatively light.
Existing technology is mainly cultural control to the control measure of Peach crown gall, and cultural control is selected namely to give up
Soil or the orchard that root knot has occurred still not can avoid the propagation of pathogen in fact in this way, palliative.In addition mostly to be
By chemical control, sodium hypochlorite, copper sulphate, sulphur powder, formalin etc. can be generally used during chemical control
Peach nursery stock, root system or Rhizosphere Soil are handled, control efficiency is general, and the ecological environment that is added to of chemical reagent is brought very greatly
Harm, therefore we need to develop a kind of method of safe and efficient, environmentally friendly prevention and treatment Peach crown gall.
Summary of the invention
The purpose of the present invention is being directed to existing problem, a kind of seed treatment for improving Peach crown gall disease incidence is provided
Method, the present invention handle peach seed, effectively improve the characteristic of peach seed, can not only pre- preventing peach root
Carninomatosis, also improves its germination percentage and disease-resistant rate, robust growth, and nontoxic to environment, or even plays to soil remediation
Certain positive effect has good market popularization value.
The present invention is achieved by the following technical solutions:
A kind of method for treating seeds improving Peach crown gall disease incidence, comprising the following steps:
(1) preparation of seed-soaking liquid:
A. it is weighed by corresponding parts by weight: 13 ~ 15 parts of tealeaves, 10 ~ 12 parts of folium artemisiae argyi, 8 ~ 10 parts of pine needle, 4 ~ 6 parts of aloe, shrub althea flower 3 ~
5 parts, 2 ~ 3 parts of tripterygium root, 2 ~ 3 parts of Radix Astragali, 1 ~ 2 part of dandelion, 0.7 ~ 0.9 part of beggar-ticks；
B. weighed each component in operation a is collectively disposed in vacuum freeze drier and is dried, vacuum freeze drier is cold
Freezing temperature is -40 ~ -30 DEG C, and cooling time is 6 ~ 10min, and drying temperature is 60 ~ 70 DEG C, and the dry time is 2 ~ 3h；
C. by operate in b it is dry after each component be added in deep cooling type pulverizer jointly, the revolving speed of pulverizer is 400 ~
The time of 500rpm, crushing are 55 ~ 65min, smash it through 100 mesh screens, weigh each component mix powder weight；
D. mix powder is placed in high pressure homogenizer after crushing in operation c, and purified water, fibre are then added into homogenizer
Plain enzyme and pectase are tieed up, the pressure of homogenizer is 20000 ~ 26000psi, and the time of homogeneous is 1 ~ 4min；
E. the mixed liquor for operating in d after homogeneous is added in centrifuge tube, then by centrifuge tube be placed in a centrifuge progress from
The heart, the revolving speed of centrifuge are 10000 ~ 12000rpm, and centrifugation time is 15 ~ 21min, after the completion of centrifugation, are taken upper in centrifuge tube
It is clearly seed-soaking liquid；
(2) seed soaking: the seed-soaking liquid that step (1) obtains is placed in electrophoresis tank, peach seed is then put into electrophoresis tank
Interior, the voltage of electrophoresis seed soaking is 100 ~ 160V, spare after the 25 ~ 31min that soaks seed；
(3) ultraviolet irradiation: the peach seed in step (2) after seed-soaking liquid is soaked seed is placed in tiling in culture dish and is opened, then
Culture dish is placed under ultraviolet lamp and is irradiated.
Further, the parts by weight of purified water are to operate the mix powder obtained in c in operation d in the step (1)
7 ~ 8 times of total weight parts.
Further, the parts by weight of operation d cellulase are to operate the mixture powder obtained in c in the step (1)
The 1 ~ 2% of last total weight parts, the middle parts by weight for operating pectase in d of the step (1) are to operate the mix powder obtained in c
The 0.5 ~ 1% of total weight parts.
Further, wavelength ultraviolet in the step (3) is 200 ~ 400nm, and the time of ultraviolet irradiation is 10 ~ 12min.
The present invention has the advantage that compared with prior art
The constituent of seed-soaking liquid of the invention is all to select Chinese herbal medicine, can be effectively prevented from inorganic chemical reagent harm
The phenomenon that environmental and human health impacts, while being also less prone to lead to the problem of disease resistance, but if only by Chinese herbal medicine to peach
The sub- simple process of tree species, using effect is bad, therefore the present invention improves conventional Chinese herbal medicine processing kind submode,
Specially select tealeaves, folium artemisiae argyi, pine needle, aloe, shrub althea flower, tripterygium root, Radix Astragali, dandelion, beggar-ticks as seed-soaking liquid
Constituent, above-mentioned most of ingredients used all have good anti-microbial property, and it is cold that each medicinal herb components are carried out vacuum
Be lyophilized it is dry, on the one hand be concentrated effective component, another aspect vacuum freeze drying is to make the one of material dewatering using the principle of distillation
Kind dry technology, can guarantee that each effective component is not destroyed, since the technology is in low temperature and condition of high vacuum degree shape to greatest extent
It is carried out under state, enzyme and bacterium are not easy to breed, and the seed-soaking liquid safety prepared is higher；Traditional Chinese medicine ingredients after drying are crushed
Afterwards, water, pectase, cellulase is added and carries out homogenized, most cells can be made in each Chinese medicine broken, release effectively at
Point, the seed-soaking liquid prepared almost is enriched all effective components required for us can improve seed for soaking seed well
Disease resistance increase the germination percentage of seed especially to Peach crown gall, effectively prevention root knot, the effective use to Chinese medicine
Degree reaches maximum and nontoxic to environment, it might even be possible to which rehabilitating soil is protected from resource has largely been saved
Environment；Traditional seed soaking mode is that seed is immersed to seed-soaking liquid, and soaked overnight, effect is general, and the present invention sets seed-soaking liquid
In electrophoresis tank, then seed is immersed, accelerates the movement and activity of effective component chemical substance by electrophoresis, thus very
The effect of getting well the low surface for being attached to seed, enhancing seed-soaking liquid, the current field condition of suitable parameters can improve the sprouting of seed
Characteristic improves its germination percentage and planting percent；It is last to have carried out ultraviolet irradiation solidification again, enhance the effective component and seed of seed-soaking liquid
Inner tissue's ingredient, which combines, to be fixed, and the effect of prevention root knot is not only acted as, moreover it is possible to be prevented and treated root knot, be blocked root knot pathogen
It propagates.The present invention handles peach seed, effectively improves the characteristic of peach seed, can not only pre- preventing peach root
Carninomatosis, has also proposed its germination percentage and disease resistance, robust growth, and nontoxic to environment, or even plays one to soil remediation
Fixed positive effect has good market popularization value.
Specific embodiment
Embodiment 1
A kind of method for treating seeds improving Peach crown gall disease incidence, comprising the following steps:
(1) preparation of seed-soaking liquid:
A. it is weighed by corresponding parts by weight: 13 parts of tealeaves, 10 parts of folium artemisiae argyi, 8 parts of pine needle, 4 parts of aloe, 3 parts of shrub althea flower, tripterygium root 2
Part, 2 parts of Radix Astragali, 1 part of dandelion, 0.7 part of beggar-ticks；
B. weighed each component in operation a is collectively disposed in vacuum freeze drier and is dried, vacuum freeze drier is cold
Freezing temperature is -40 DEG C, cooling time 6min, and drying temperature is 60 DEG C, and the dry time is 2h；
C. each component after will be dry in operation b is added to jointly in deep cooling type pulverizer, and the revolving speed of pulverizer is 400rpm,
The time of crushing is 55min, smashes it through 100 mesh screens, weighs each component mix powder weight；
D. mix powder is placed in high pressure homogenizer after crushing in operation c, and purified water, fibre are then added into homogenizer
Plain enzyme and pectase are tieed up, the pressure of homogenizer is 20000psi, and the time of homogeneous is 1min；
E. the mixed liquor for operating in d after homogeneous is added in centrifuge tube, then by centrifuge tube be placed in a centrifuge progress from
The revolving speed of the heart, centrifuge is 10000rpm, centrifugation time 15min, and after the completion of centrifugation, taking the supernatant in centrifuge tube is to soak seed
Liquid；
(2) seed soaking: the seed-soaking liquid that step (1) obtains is placed in electrophoresis tank, peach seed is then put into electrophoresis tank
Interior, the voltage of electrophoresis seed soaking is 100V, spare after the 25min that soaks seed；
(3) ultraviolet irradiation: the peach seed in step (2) after seed-soaking liquid is soaked seed is placed in tiling in culture dish and is opened, then
Culture dish is placed under ultraviolet lamp and is irradiated.
Further, the parts by weight of purified water are to operate the mix powder obtained in c in operation d in the step (1)
7 times of total weight parts.
Further, the parts by weight of operation d cellulase are to operate the mixture powder obtained in c in the step (1)
The 1% of last total weight parts, the parts by weight of pectase be that the mix powder that obtains in operation c is total in operation d in the step (1)
The 0.5% of parts by weight.
Further, wavelength ultraviolet in the step (3) is 200nm, and the time of ultraviolet irradiation is 10min.
Embodiment 2
A kind of method for treating seeds improving Peach crown gall disease incidence, comprising the following steps:
(1) preparation of seed-soaking liquid:
A. it is weighed by corresponding parts by weight: 14 parts of tealeaves, 11 parts of folium artemisiae argyi, 9 parts of pine needle, 5 parts of aloe, 4 parts of shrub althea flower, tripterygium root
2.5 parts, 2.5 parts of Radix Astragali, 1.5 parts of dandelion, 0.8 part of beggar-ticks；
B. weighed each component in operation a is collectively disposed in vacuum freeze drier and is dried, vacuum freeze drier is cold
Freezing temperature is -35 DEG C, cooling time 8min, and drying temperature is 65 DEG C, and the dry time is 2.5h；
C. each component after will be dry in operation b is added to jointly in deep cooling type pulverizer, and the revolving speed of pulverizer is 450rpm,
The time of crushing is 60min, smashes it through 100 mesh screens, weighs each component mix powder weight；
D. mix powder is placed in high pressure homogenizer after crushing in operation c, and purified water, fibre are then added into homogenizer
Plain enzyme and pectase are tieed up, the pressure of homogenizer is 23000psi, and the time of homogeneous is 2.5min；
E. the mixed liquor for operating in d after homogeneous is added in centrifuge tube, then by centrifuge tube be placed in a centrifuge progress from
The revolving speed of the heart, centrifuge is 11000rpm, centrifugation time 18min, and after the completion of centrifugation, taking the supernatant in centrifuge tube is to soak seed
Liquid；
(2) seed soaking: the seed-soaking liquid that step (1) obtains is placed in electrophoresis tank, peach seed is then put into electrophoresis tank
Interior, the voltage of electrophoresis seed soaking is 130V, spare after the 28min that soaks seed；
(3) ultraviolet irradiation: the peach seed in step (2) after seed-soaking liquid is soaked seed is placed in tiling in culture dish and is opened, then
Culture dish is placed under ultraviolet lamp and is irradiated.
Further, the parts by weight of purified water are to operate the mix powder obtained in c in operation d in the step (1)
7.5 times of total weight parts.
Further, the parts by weight of operation d cellulase are to operate the mixture powder obtained in c in the step (1)
The 1.5% of last total weight parts, the middle parts by weight for operating pectase in d of the step (1) are to operate the mix powder obtained in c
The 0.75% of total weight parts.
Further, wavelength ultraviolet in the step (3) is 300nm, and the time of ultraviolet irradiation is 11min.
Embodiment 3
A kind of method for treating seeds improving Peach crown gall disease incidence, comprising the following steps:
(1) preparation of seed-soaking liquid:
A. it is weighed by corresponding parts by weight: 15 parts of tealeaves, 12 parts of folium artemisiae argyi, 10 parts of pine needle, 6 parts of aloe, 5 parts of shrub althea flower, tripterygium root
3 parts, 3 parts of Radix Astragali, 2 parts of dandelion, 0.9 part of beggar-ticks；
B. weighed each component in operation a is collectively disposed in vacuum freeze drier and is dried, vacuum freeze drier is cold
Freezing temperature is -30 DEG C, cooling time 10min, and drying temperature is 70 DEG C, and the dry time is 3h；
C. each component after will be dry in operation b is added to jointly in deep cooling type pulverizer, and the revolving speed of pulverizer is 500rpm,
The time of crushing is 65min, smashes it through 100 mesh screens, weighs each component mix powder weight；
D. mix powder is placed in high pressure homogenizer after crushing in operation c, and purified water, fibre are then added into homogenizer
Plain enzyme and pectase are tieed up, the pressure of homogenizer is 26000psi, and the time of homogeneous is 4min；
E. the mixed liquor for operating in d after homogeneous is added in centrifuge tube, then by centrifuge tube be placed in a centrifuge progress from
The revolving speed of the heart, centrifuge is 12000rpm, centrifugation time 21min, and after the completion of centrifugation, taking the supernatant in centrifuge tube is to soak seed
Liquid；
(2) seed soaking: the seed-soaking liquid that step (1) obtains is placed in electrophoresis tank, peach seed is then put into electrophoresis tank
Interior, the voltage of electrophoresis seed soaking is 160V, spare after the 31min that soaks seed；
(3) ultraviolet irradiation: the peach seed in step (2) after seed-soaking liquid is soaked seed is placed in tiling in culture dish and is opened, then
Culture dish is placed under ultraviolet lamp and is irradiated.
Further, the parts by weight of purified water are to operate the mix powder obtained in c in operation d in the step (1)
8 times of total weight parts.
Further, the parts by weight of operation d cellulase are to operate the mixture powder obtained in c in the step (1)
The 2% of last total weight parts, the parts by weight of pectase be that the mix powder that obtains in operation c is total in operation d in the step (1)
The 1% of parts by weight.
Further, wavelength ultraviolet in the step (3) is 400nm, and the time of ultraviolet irradiation is 12min.
Comparative example 1
This comparative example 1 compared with Example 2, saves the pectase and fibre in the operation d in the preparation of step (1) seed-soaking liquid
Plain enzyme is tieed up, method and step in addition to this is all the same.
Comparative example 2
This comparative example 2 changes conventional seed soaking side into compared with Example 2, by the electrophoresis seed soaking in step (2) seed soaking
Formula, method and step in addition to this are all the same.
Comparative example 3
This comparative example 3 compared with Example 2, saves step (3) ultraviolet irradiation, and method and step in addition to this is all the same.
Comparative example 4
This comparative example 4 changes conventional seed soaking side into compared with Example 2, by the electrophoresis seed soaking in step (2) seed soaking
Formula, while step (3) ultraviolet irradiation is saved, method and step in addition to this is all the same.
Control group
Existing peach seed chemical reagent immersion treatment method, specific chemical reagent are sodium hypochlorite (containing 5% effective chlorine composition)
Solution.
In order to compare effect of the present invention, using the seed of the peach of the same a batch picking in the same orchard as experimental subjects, so
Use above-described embodiment 2, comparative example 1, comparative example 2, comparative example 3, comparative example 4, control group institute respectively afterwards
The method processing peach seed stated, and sets one group of blank control group without any processing, after the completion to treated peach kind
Son carries out planting experiment, and during which control growth management condition is identical and suitable, and specific growth correlation data is as shown in table 1 below:
Table 1
| Root knot illness rate (%) | Control efficiency (%) | |
| Embodiment 2 | 0.79 | 97.3 |
| Comparative example 1 | 5.27 | 81.9 |
| Comparative example 2 | 7.64 | 73.8 |
| Comparative example 3 | 1.96 | 93.2 |
| Comparative example 4 | 11.21 | 61.6 |
| Control group | 9.85 | 66.2 |
| Blank control group | 29.17 | / |
Note: control efficiency (%)=(blank control group root knot illness rate-processing group root knot illness rate) described in upper table 1/
Blank control group root knot illness rate × 100.
The present invention handles peach seed it can be seen from upper table 1, effectively improves the spy of peach seed
Property, Peach crown gall can not only be prevented, also improve its germination percentage and disease resistance, robust growth, and to the nontoxic nothing of environment
Evil, or even certain positive effect is played to soil remediation, there is good market popularization value.
Claims (4)
1. a kind of method for treating seeds for improving Peach crown gall disease incidence, which comprises the following steps:
(1) preparation of seed-soaking liquid:
A. it is weighed by corresponding parts by weight: 13 ~ 15 parts of tealeaves, 10 ~ 12 parts of folium artemisiae argyi, 8 ~ 10 parts of pine needle, 4 ~ 6 parts of aloe, shrub althea flower 3 ~
5 parts, 2 ~ 3 parts of tripterygium root, 2 ~ 3 parts of Radix Astragali, 1 ~ 2 part of dandelion, 0.7 ~ 0.9 part of beggar-ticks；
B. weighed each component in operation a is collectively disposed in vacuum freeze drier and is dried, vacuum freeze drier is cold
Freezing temperature is -40 ~ -30 DEG C, and cooling time is 6 ~ 10min, and drying temperature is 60 ~ 70 DEG C, and the dry time is 2 ~ 3h；
C. by operate in b it is dry after each component be added in deep cooling type pulverizer jointly, the revolving speed of pulverizer is 400 ~
The time of 500rpm, crushing are 55 ~ 65min, smash it through 100 mesh screens, weigh each component mix powder weight；
D. mix powder is placed in high pressure homogenizer after crushing in operation c, and purified water, fibre are then added into homogenizer
Plain enzyme and pectase are tieed up, the pressure of homogenizer is 20000 ~ 26000psi, and the time of homogeneous is 1 ~ 4min；
E. the mixed liquor for operating in d after homogeneous is added in centrifuge tube, then by centrifuge tube be placed in a centrifuge progress from
The heart, the revolving speed of centrifuge are 10000 ~ 12000rpm, and centrifugation time is 15 ~ 21min, after the completion of centrifugation, are taken upper in centrifuge tube
It is clearly seed-soaking liquid；
(2) seed soaking: the seed-soaking liquid that step (1) obtains is placed in electrophoresis tank, peach seed is then put into electrophoresis tank
Interior, the voltage of electrophoresis seed soaking is 100 ~ 160V, spare after the 25 ~ 31min that soaks seed；
(3) ultraviolet irradiation: the peach seed in step (2) after seed-soaking liquid is soaked seed is placed in tiling in culture dish and is opened, then
Culture dish is placed under ultraviolet lamp and is irradiated.
2. a kind of method for treating seeds for improving Peach crown gall disease incidence according to claim 1, which is characterized in that described
The parts by weight of purified water are 7 ~ 8 times for operating the mix powder total weight parts obtained in c in operation d in step (1).
3. a kind of method for treating seeds for improving Peach crown gall disease incidence according to claim 1, which is characterized in that described
The parts by weight of operation d cellulase are operate the mix powder total weight parts obtained in c 1 ~ 2% in step (1), described
The parts by weight of pectase are operate the mix powder total weight parts obtained in c 0.5 ~ 1% in operation d in step (1).
4. a kind of method for treating seeds for improving Peach crown gall disease incidence shown according to claim 1, which is characterized in that described
Ultraviolet wavelength is 200 ~ 400nm in step (3), and the time of ultraviolet irradiation is 10 ~ 12min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655605A (en) * | 2020-12-31 | 2021-04-16 | 阜南县京淮特种水产有限公司 | Method for breeding willow-root fish with high survival rate and low morbidity |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106817955A (en) * | 2016-12-30 | 2017-06-13 | 吕福贵 | A kind of processing method of corn seed |
| CN106954514A (en) * | 2017-03-31 | 2017-07-18 | 镇远县醉美果业有限公司 | A kind of method for culturing seedlings of yellow peach |
| CN107548928A (en) * | 2017-09-22 | 2018-01-09 | 界首市靳寨乡红星家庭农场 | A kind of implantation methods of greenhouse organic spinach |
-
2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106817955A (en) * | 2016-12-30 | 2017-06-13 | 吕福贵 | A kind of processing method of corn seed |
| CN106954514A (en) * | 2017-03-31 | 2017-07-18 | 镇远县醉美果业有限公司 | A kind of method for culturing seedlings of yellow peach |
| CN107548928A (en) * | 2017-09-22 | 2018-01-09 | 界首市靳寨乡红星家庭农场 | A kind of implantation methods of greenhouse organic spinach |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655605A (en) * | 2020-12-31 | 2021-04-16 | 阜南县京淮特种水产有限公司 | Method for breeding willow-root fish with high survival rate and low morbidity |
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Application publication date: 20181207 |