CN108913640A - A kind of complex micro organism fungicide and its application for bamboo charcoal - Google Patents
A kind of complex micro organism fungicide and its application for bamboo charcoal Download PDFInfo
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Abstract
The invention discloses a kind of complex micro organism fungicides for bamboo charcoal fermentation and its application, the complex micro organism fungicide to include in parts by weight：1-6 parts of bacillus subtilis, 1-6 parts of actinomyces, 5-15 parts of lactic acid bacteria, 2-8 parts of saccharomycete, 1-6 parts of phosphate solubilizing bacteria and 1-6 parts of potassium solubilizing bacteria.Complex micro organism fungicide formula is simple, and ferment effect is good, and preparation method is simple, and operating process is easy, and preparation condition is mild, and easy to accomplish and control is easy to largely be prepared, can effectively be fermented, be worked well to bamboo charcoal.
Description
Technical field
The invention belongs to technical field of microbial fermentation, it is related to a kind of complex micro organism fungicide and its application, and in particular to
It is a kind of for bamboo charcoal fermentation complex micro organism fungicide and its application more particularly to it is a kind of for bamboo charcoal fermentation microbial bacterial agent
And its fertilizer of preparation, the preparation method and application of fertilizer.
Background technique
With the numerous areas application of China's agricultural development and bamboo wood industry, bamboo kind resource, bamboo grove area and accumulation are every
Year is gradually increasing, and people utilize a variety of extensions of bamboo wood is surging forward also with consumption demand, bamboo wood plantation and development and utilization
To plant based on processing and utilization, therefore huge bamboo wastes are produced, because of bamboo cellulose, hemicellulose, lignin
Equal substances decompose slowly in the soil, need specific bacteria agent carry out specific aim decompose it is decomposed, could by soil receiving with
Root system of plant absorbs.
Salt-soda soil is important land resource, the salt-soda soil in China be mainly distributed on northeast, North China, Northwest inland area with
And North of Yangtze River coastal area, development and utilization salt-soda soil can not only bring more land under cultivation, and increase yield per unit area, improve grain
Total output is eaten, and green coverage can be expanded, improves ecological environment, thus needs to find acid-base balance in a kind of adjusting soil
Method.
104529612 A of CN discloses a kind of preparation method of lactic acid bacteria liquid fertilizer, and this method is mixed according to formula rate
White granulated sugar, brown sugar, soybean powder, xanthan gum, converted starch, sucrose ester, sodium pyrophosphate and sodium dihydrogen phosphate are closed, and upon mixing
Lactic acid bacteria and streptococcus are added in material.This method simple process and low cost can utilize the soil in lactic acid office improvement salt-soda soil
Physicochemical character, but culture fertility, the effect for repairing farmland and raising crop yield are bad.
105586043 A of CN discloses a kind of saline-alkali soil conditioner and its preparation and application, the modifying agent major ingredient include
Humus and/or sludge, ingredient are complex micro organism fungicide, include lactic acid bacteria, saccharomycete, unwrapping wire in the complex micro organism fungicide
Bacterium, nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium solubilizing bacteria.The modifying agent can improve the acid-base imbalance of soil, but culture fertility, raising make produce
The effect of amount and quality is limited.
107141105 A of CN discloses a kind of compound fertilizer and preparation method, which includes potassium dihydrogen phosphate, crosses phosphorus
Sour calcium, ammonium nitrate, urea, potassium chloride, bacillus subtilis, lactic acid bacteria, saccharomycete, actinomyces, nitrogen-fixing bacteria, potassium solubilizing bacteria, phosphorus decomposing
Bacterium, zinc sulfate, magnesium sulfate, copper sulphate and manganese sulfate, the compound fertilizer which provides can carry out the benefit of microelement to soil
It fills, but action time is short, is easy to run off.
Charcoal typically refers to the biomass such as agriculture and forestry organic waste material, and the rich carbon of formation is pyrolyzed under the conditions of anoxic and certain temperature
Product.Charcoal can be used to solve straw burning problem, reduce pollution of area source, realize agriculture " carbon sequestration ", improvement soil, training
Fertile soil fertility repairs farmland, improves crop yield and quality.Bamboo charcoal is one of charcoal, and China's bamboo resource is abundant, a large amount of bamboo
Product processing enterprise arises, and a large amount of leftover bits and pieces can be generated in bamboo product process, these leftover bits and pieces are by being fired into
Bamboo charcoal makes resource be fully utilized.
Chen Chongjun etc. is in Zhejiang Agriculture journal, 2018,30 (1)：123-128 has delivered entitled addition bamboo charcoal to layout planning
Soil nitrogen phosphorus be lost and microbiologic population influence, the research discovery bamboo charcoal can increase Phylogenetic diversity of bacteria, but not to bamboo charcoal with
The mixing collaboration of bacterium is studied.
104355932 A of CN discloses a kind of Biologic organic bamboo charcoal fertilizer and its production method, this method by bamboo charcoal powder, have
Machine compost, corrosive acid, amino acid, biological inoculum, powdery bacterium powder carrier are mixed to prepare according to producting proportion metering, but this method institute
It is one of bacillus subtilis, bacillus laterosporus, bacillus megaterium, EM bacterium or colloid bacillus cereus with biological inoculum
Or two or more any mixing, these strains can not decompose the fixed substance contained in bamboo powder, can not discharge the function of multiplicity
Nutrient realizes that nutrient is recycled in soil, root system of plant.But a kind of bamboo charcoal fermenting agent with strong points currently not yet, therefore,
It is particularly significant to solve the exclusive microbial inoculum of bamboo charcoal fermentation.
Therefore, it needs to carry out compound criteria upgrading to strain in improving bamboo charcoal production fermentation process, forming one kind has
Multi-functional microbial bacterial agent, can quickly decompose bamboo charcoal, can improve crumb structure to soil, can to root system of plant
Excellent micro-ecological environment is formed, realizes the multifunctional comprehensive synergy target of microbial inoculum.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of complex micro organism fungicide for bamboo charcoal fermentation and its application, this is multiple
Conjunction microbial bacteria agent prescription is simple, and ferment effect is good, and preparation method is simple, and operating process is easy, and preparation condition is mild, is easy real
It now and controls, is easy to largely be prepared, can effectively be fermented, be worked well to bamboo charcoal.
For this purpose, the present invention uses following technical scheme：
On the one hand, the present invention provides a kind of complex micro organism fungicide for bamboo charcoal fermentation, the complex micro organism fungicide
Include in parts by weight：1-6 parts of bacillus subtilis, 1-6 parts of actinomyces, 5-15 parts of lactic acid bacteria, 2-8 parts of saccharomycete, phosphate solubilizing bacteria
1-6 parts and potassium solubilizing bacteria 1-6 parts.
In the present invention, the actinomyces can decompose hemicellulose or wooden equal complex compounds, moreover it is possible to promote Zinc fractions
Crumb structure and improve soil；The bacillus subtilis can generate subtilin, polymyxins, nystatin and gramicidins
Isoreactivity substance, it is strong to soil pathogenic bacteria inhibiting effect, soil environment can be improved；The lactic acid bacteria can make indissoluble in soil
Nutrient Transformation, conducive to being absorbed and utilized for crop, and the pH value of the adjustable alkaline land soil of lactic acid bacteria tends to neutral soil；Institute
Fermenting speed can be accelerated by stating saccharomycete, and the mycelium of saccharomycete can be used as the channel of bacterial migration, solves bacterium and exists
Difficult problem is migrated in soil；The phosphate solubilizing bacteria can convert the phosphorus for being difficult to be absorbed and utilized in plant to absorbable utilize
Form, the increase of carbon source are conducive to the existence and breeding of phosphate solubilizing bacteria, and phosphate solubilizing bacteria and actinomyces, which are used cooperatively, to be conducive to improve phosphorus decomposing
The phosphorus decomposing effect of bacterium；Potassium solubilizing bacteria can discharge the slowly available potassium in soil, additionally it is possible to which activating rhizosphere soil microenvironment improves soil
Earth environment；Complex micro organism fungicide provided by the invention including the above strain, six plants of bacterial strains during the fermentation, different strains
Although being employed to carbon source, nitrogen source, energy substance etc., being different bacterial strain can mutually be cooperateed with, and can be filled when fermentation
Point using the nutriment in culture medium, obtain the metabolite and promoted mutually, can fast decoupled bamboo charcoal, conditioned soil
Earth crumb structure forms good micro-ecological environment for biological root system, realizes the multifunctional comprehensive synergy target of microbial inoculum.
Described bacillus subtilis 1-6 parts, such as can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts, preferably 1-3 parts；
Described actinomyces 1-6 parts, such as can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts, preferably 1-3 parts；The lactic acid bacteria 5-15
Part, such as can be 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts or 15 parts, preferably 6-10 parts；
Described saccharomycete 2-8 parts, such as can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts or 8 parts, preferably 3-6 parts；The phosphate solubilizing bacteria
1-6 parts, such as can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts, preferably 1-3 parts；Described potassium solubilizing bacteria 1-6 parts, such as can be with
It is 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts, preferably 1-3 parts.
According to the present invention, the complex micro organism fungicide further includes auxiliary material.
In the present invention, inventors have found that cooperating by complex micro organism fungicide and auxiliary material, moisture and nutriment can be stored,
Retain water and nutrients, to promote the growth and breeding of microorganism.
Preferably, the auxiliary material be fulvic acid, humic acid or bamboo acetic acid in any one or at least two combination, allusion quotation
Type but unrestricted combination include the combination of fulvic acid and humic acid, the combination of fulvic acid and bamboo acetic acid, humic acid and bamboo vinegar
The combination of acid and the combination of fulvic acid, humic acid and bamboo acetic acid, the preferably combination of fulvic acid, humic acid and bamboo acetic acid.
In the present invention, inventor is by screening a large amount of auxiliary materials, and discovery is using fulvic acid, humic acid and bamboo acetic acid specific
Under the cooperation of parts by weight, by increasing effective N, P, K component in soil, increases the nutrient of soil, can further promote
Plant growth, raising plant stress-resistance ability, increases plant products, promotes absorption of the root system to phosphorus, further by auxiliary material and again
The cooperation for closing microbial bacterial agent can preferably improve soil environment, shorten fermentation time, promote the fermentation of bamboo charcoal.
Preferably, the fulvic acid accounts for 3-15 part of the complex micro organism fungicide in parts by weight, such as can be 3
Part, 5 parts, 6 parts, 8 parts, 10 parts, 12 parts or 15 parts, preferably 5-10 parts.
Preferably, the humic acid accounts for 10-40 part of the complex micro organism fungicide in parts by weight, such as can be 10
Part, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts or 40 parts, preferably 15-30 parts.
Preferably, the bamboo acetic acid accounts for 3-15 part of the complex micro organism fungicide in parts by weight, such as can be 3
Part, 5 parts, 6 parts, 8 parts, 10 parts, 12 parts or 15 parts, preferably 6-10 parts.
Second aspect, the present invention provides a kind of complex micro organism fungicides as described in relation to the first aspect to be used to prepare bamboo charcoal hair
The purposes of the fertilizer of ferment.
In the present invention, bamboo charcoal has porous structure, cooperates above-mentioned auxiliary material, can further increase the nutrient of soil, not only
In this way, bamboo charcoal also has high adsorption capacity, cooperates auxiliary material, the release of fertilizer nutrient in the soil can be delayed, while can show
The effects of improving soil texture, keeping soil from packing together is write, to be conducive to the growth of strain.
Preferably, the bamboo charcoal accounts for 50-100 part of the complex micro organism fungicide in parts by weight, such as can be 50
Part, 60 parts, 70 parts, 80 parts, 85 parts, 90 parts or 100 parts, preferably 60-85 parts.
As optimal technical scheme, the fertilizer includes the following raw material in parts by weight：1-6 parts of bacillus subtilis is put
1-6 parts of line bacterium, 5-15 parts of lactic acid bacteria, 2-8 parts of saccharomycete, 1-6 parts of phosphate solubilizing bacteria, 1-6 parts of potassium solubilizing bacteria, 50-100 parts of bamboo charcoal, yellow corruption
3-15 parts sour, 10-40 parts of humic acid and 3-15 parts of bamboo acetic acid.
It is further preferred that the fertilizer includes the following raw material in parts by weight：1-3 parts of bacillus subtilis, actinomyces
1-3 parts, 6-10 parts of lactic acid bacteria, 3-6 parts of saccharomycete, 1-3 parts of phosphate solubilizing bacteria, 1-3 parts of potassium solubilizing bacteria, 60-85 parts of bamboo charcoal, fulvic acid 5-10
Part, 15-30 parts of humic acid and 6-10 parts of bamboo acetic acid.
The third aspect, the present invention provides the preparation method of fertilizer as described above, the preparation method includes the following steps：
(1) bacterium solution is carried out by formula ratio respectively carrying out cultivation and fermentation in mixing addition bamboo charcoal, obtains fermenting agent；
(2) fertilizer material is added in the fermentation liquid of step (1) by formula ratio and stirs and carry out two stages fermentation, obtain institute
State complex micro organism fungicide.
In the preparation method of fertilizer of the present invention, the temperature of step (1) described fermented and cultured is 20-35 DEG C, such as
It can be 20 DEG C, 25 DEG C, 30 DEG C, 32 DEG C or 35 DEG C, preferably 25-32 DEG C.
Preferably, the time of step (1) described fermentation be 8-18h, such as can be 8h, 10h, 12h, 14h, 16h or
18h, preferably 10-14h.
Preferably, the cell density of fermenting agent obtained by step (1) is 90-120cfu/mL, such as can be 90cfu/
ML, 100cfu/mL, 103cfu/mL, 110cfu/mL, 115cfu/mL, 118cfu/mL or 120cfu/mL, preferably 103-
118cfu/mL。
The required fermenting agent of the available present invention under the cooperation of cultivation and fermentation temperature and time provided by the present invention
Cell density, under the cell density each strain activity preferably, for subsequent two stages fermentation lay the foundation.
Preferably, the time of the stirring is 20-50h, such as can be 20h, 25h, 30h, 35h, 40h, 45h or 50h,
Preferably 30-40h.
Mixing time selected by the present invention can guarantee that filtrated air is sufficiently mixed with fermentation liquid, can guarantee fermentation liquid
Dissolved oxygen required for middle micro-organisms breeding and metabolism.
The first fermentation in the two stages fermentation is aerobic fermentation.
Contain a large amount of hole in bamboo charcoal, the carbon dioxide gas that the aerobic fermentation in the present invention generates promotes thallus and bamboo
The mixing of charcoal, and the temperature that aerobic fermentation process generates can consume the carbohydrate energy in complex micro organism fungicide
Amount avoids bring when fertilising from burning root phenomenon；Further, consuming carbohydrate can be improved prepared composite microbial
The carbon-nitrogen ratio of object microbial inoculum avoids the contention of microorganism and soil to nitrogen source, improves soil physical factors.
Preferably, the temperature of first fermentation is 35-60 DEG C, such as can be 35 DEG C, 40 DEG C, 45 DEG C, 50 DEG C, 52
DEG C, 55 DEG C or 60 DEG C, preferably 40-52 DEG C.
Preferably, the time of first fermentation is 2-6 days, such as can be 2 days, 3 days, 4 days, 5 days or 6 days, preferably
It is 3-5 days.
Preferably, further include the steps that heat preservation after first fermentation.
Preferably, the temperature of the heat preservation is 40-60 DEG C, such as can be 40 DEG C, 45 DEG C, 50 DEG C, 52 DEG C, 55 DEG C or 60
DEG C, preferably 40-52 DEG C, preferably 40-52 DEG C.
Preferably, the time of the heat preservation is 1-5 days, such as can be 1 day, 2 days, 3 days, 4 days or 5 days, preferably 2-3
It.
Preferably, the second fermentation in the two stages fermentation is anaerobic fermentation.
Preferably, the temperature of second fermentation is 40-65 DEG C, such as can be 40 DEG C, 45 DEG C, 50 DEG C, 52 DEG C, 59
DEG C, 60 DEG C or 65 DEG C, preferably 50-59 DEG C.
Preferably, the time of second fermentation is 2-6 days, such as can be 2 days, 3 days, 4 days, 5 days or 6 days, preferably
It is 3-5 days.
It further include the pretreated step of bamboo charcoal before step (1).
Preferably, the pretreatment obtains the bamboo charcoal for bamboo is carried out thermal cracking.
The available a certain amount of bamboo acetic acid of thermal cracking bamboo charcoal, and the bamboo charcoal specific surface area by thermal cracking processing increases
Greatly, more biological bacterias can be adsorbed, in the present invention, fermentation time reduction needed for the bamboo charcoal handled by thermal cracking, therefore
Thermal cracking can be improved fermentation efficiency.
Preferably, the temperature of the thermal cracking is 300-500 DEG C, such as can be 300 DEG C, 350 DEG C, 400 DEG C, 450 DEG C
Or 500 degrees Celsius, preferably 350-450 DEG C.
As the preferred technical solution of the present invention, the present invention provides the preparation method of such as above-mentioned complex micro organism fungicide,
The preparation method includes the following steps：
It (1) is that 300-500 DEG C of thermal cracking high mountain moso bamboo obtains bamboo charcoal in temperature；
(2) bacterium solution mixing is carried out by formula ratio respectively to be added in bamboo charcoal in 20-35 DEG C of temperature progress cultivation and fermentation 8-
18h, obtains fermenting agent, and cell density is 90-120/mL；
(2) auxiliary material is mixed and is sterilized, stir 30-40min, and be added in the fermentation liquid of step (1) by formula ratio and stir 20-
50h, and two stages fermentation is carried out, tunning is dissolved in water, the humidity of obtained lysate is 40-55%, dries, divides
The complex micro organism fungicide is prepared in choosing, crushing；
Wherein, two stages fermentation is divided into the first fermentation and the second fermentation, and the temperature of the first fermentation is 35-60 DEG C, and the time is
2-6 days, 1-5 days are kept the temperature, the temperature of the second fermentation is 40-65 DEG C, and the time is 2-6 days.
On the other hand, the present invention also provides the purposes of such as above-mentioned complex micro organism fungicide.Composite microbial of the invention
Object microbial inoculum is used to prepare feed and/or feed supplementation.
Compared with prior art, the application has the advantages that：
(1) present invention can to bamboo charcoal by working in coordination for each strain by using the composite bacteria agent of specific composition
It is quickly decomposed, crumb structure can be improved to soil, excellent micro-ecological environment is capable of forming to root system of plant, realizes microbial inoculum
Multifunctional comprehensive synergy target；
(2) cooperated by using composite bacteria agent with the bamboo charcoal and auxiliary material specifically handled in the present invention, fertilizer can be delayed
Expect the release of nutrient in the soil, while can significantly improve soil texture, increase effective N, P, K component in soil, increases
The nutrient of soil shortens fermentation time, promotes the fermentation of bamboo charcoal；
(3) complex micro organism fungicide of the present invention, formula is simple, and ferment effect is good, and preparation method is simple, operating process letter
Just, preparation condition is mild, and easy to accomplish and control is easy to largely be prepared, is conducive to industrial application.
Specific embodiment
The technological means and its effect taken for the present invention is further explained, with reference to embodiments to the present invention make into
Illustrate to one step.It is understood that the specific embodiments described herein are used only for explaining the present invention, rather than to this hair
Bright restriction.
In the examples where no specific technique or condition is specified, described technology or conditions according to the literature in the art,
Or it is carried out according to product description.Reagents or instruments used without specified manufacturer, be can be by regular channel commercially available from
The conventional products of acquisition.
Embodiment one
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 3 parts of bacillus subtilises, 3 parts of actinomyces, 8 parts of lactic acid bacterias, 4 parts of saccharomycete,
3 parts of phosphate solubilizing bacterias and 3 parts of potassium solubilizing bacteria mixing, it is 50 parts without thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of fermented and cultured is 20 DEG C, fermentation time 8h, obtains the fermentation that cell density is 90cfu/mL
Microbial inoculum；
Fermenting agent is dissolved in water, the humidity for obtaining lysate is 40%, dries, sorts, crushing institute is prepared
State complex micro organism fungicide.
Embodiment two
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Microbial inoculum is weighed according to parts by weight, by 5 parts of bacillus subtilises, 5 parts of actinomyces, 10 parts of lactic acid bacterias, 6 parts of yeast
Bacterium, 5 parts of phosphate solubilizing bacterias and 5 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the process thermal cracking that parts by weight are 60 parts and handle
Bamboo charcoal in ferment, the temperature of bamboo charcoal thermal cracking is 300 DEG C, and the temperature of fermented and cultured is 25 DEG C, fermentation time 10h,
Obtain the complex micro organism fungicide that cell density is 103cfu/mL.
Embodiment three
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 2 parts of bacillus subtilises, 2 parts of actinomyces, 6 parts of lactic acid bacterias, 3 parts of saccharomycete,
2 parts of phosphate solubilizing bacterias and 2 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the bamboo handled by thermal cracking that parts by weight are 85 parts
It ferments in charcoal, the temperature of bamboo charcoal thermal cracking is 350 DEG C, and the temperature of fermented and cultured is 30 DEG C, and fermentation time 12h is obtained
Cell density is the complex micro organism fungicide of 110cfu/mL.
Example IV
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Microbial inoculum is weighed according to parts by weight, by 7 parts of bacillus subtilises, 6 parts of actinomyces, 15 parts of lactic acid bacterias, 8 parts of yeast
Bacterium, 7 parts of phosphate solubilizing bacterias and 7 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the process thermal cracking that parts by weight are 50 parts and handle
Bamboo charcoal in ferment, the temperature of bamboo charcoal thermal cracking is 400 DEG C, and the temperature of fermented and cultured is 35 DEG C, fermentation time 14h,
Obtain the complex micro organism fungicide that cell density is 118cfu/mL.
Embodiment five
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 1 part of bacillus subtilis, 1 part of actinomyces, 5 parts of lactic acid bacterias, 2 parts of saccharomycete,
1 part of phosphate solubilizing bacteria and 1 part of potassium solubilizing bacteria mixing, it is 100 parts by thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of bamboo charcoal thermal cracking is 450 DEG C, and the temperature of fermented and cultured is 32 DEG C, and fermentation time 18h is obtained
The fermenting agent for being 120cfu/mL to cell density；
Fermenting agent is dissolved in water, the humidity for obtaining lysate is 45%, dries, sorts, crushing institute is prepared
State complex micro organism fungicide.
Embodiment six
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 3 parts of bacillus subtilises, 3 parts of actinomyces, 8 parts of lactic acid bacterias, 4 parts of saccharomycete,
3 parts of phosphate solubilizing bacterias and 3 parts of potassium solubilizing bacteria mixing, it is 70 parts without thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of fermented and cultured is 30 DEG C, fermentation time 12h, obtains the hair that cell density is 110cfu/mL
Yeast-like fungi agent；
8 parts of fulvic acids, 20 parts of humic acid and 8 parts of bamboo acetic acids are mixed, is added in fermenting agent after disinfection and stirs 35h, and
Two stages fermentation is carried out, the complex micro organism fungicide is obtained, wherein two stages fermentation is divided into the first fermentation and the second fermentation, the
The temperature of one fermentation is 45 DEG C, and the time of the first fermentation is 4 days, and the temperature of the second fermentation is 52 DEG C, and the time of the second fermentation is 4
It；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 45 DEG C, and the time of heat preservation is
3 days.
Embodiment seven
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 2 parts of bacillus subtilises, 2 parts of actinomyces, 6 parts of lactic acid bacterias, 3 parts of saccharomycete,
2 parts of phosphate solubilizing bacterias and 2 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the bamboo handled by thermal cracking that parts by weight are 60 parts
It ferments in charcoal, the temperature of thermal cracking is 300 DEG C, and the temperature of fermented and cultured is 32 DEG C, and fermentation time 14h obtains thallus
Density is the fermenting agent of 118cfu/mL；
5 parts of fulvic acids, 15 parts of humic acid and 6 parts of bamboo acetic acids are mixed, 40min is stirred after disinfection, is added in fermenting agent
40h is stirred, and carries out two stages fermentation, obtains the composite fermentation microbial inoculum, wherein two stages fermentation is divided into the first fermentation and the
Two fermentations, the temperature of the first fermentation are 52 DEG C, and the time of the first fermentation is 5 days, and the temperature of the second fermentation is 59 DEG C, the second fermentation
Time be 5 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 52 DEG C, and the time of heat preservation is
4 days；
Composite fermentation microbial inoculum is dissolved in water, obtained lysate humidity is 40%, and drying, sorting, crushing are prepared into
To the complex micro organism fungicide.
Embodiment eight
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Microbial inoculum is weighed according to parts by weight, by 5 parts of bacillus subtilises, 5 parts of actinomyces, 10 parts of lactic acid bacterias, 6 parts of yeast
Bacterium, 5 parts of phosphate solubilizing bacterias and 5 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the process thermal cracking that parts by weight are 85 parts and handle
Bamboo charcoal in ferment, the temperature of thermal cracking is 350 DEG C, and the temperature of fermented and cultured is 35 DEG C, and fermentation time 18h is obtained
Cell density is the fermenting agent of 120cfu/mL；
10 parts of fulvic acids, 30 parts of humic acid and 10 parts of bamboo acetic acids are mixed, 30min is stirred after disinfection, fermenting agent is added
Middle stirring 50h, and two stages fermentation is carried out, composite fermentation microbial inoculum is obtained, wherein two stages fermentation is divided into the first fermentation and second
Fermentation, the temperature of the first fermentation are 60 DEG C, and the time of the first fermentation is 6 days, and the temperature of the second fermentation is 65 DEG C, the second fermentation
Time is 6 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 60 DEG C, and the time of heat preservation is
5 days；
Composite fermentation microbial inoculum is dissolved in water, obtained lysate humidity is 50%, and drying, sorting, crushing are prepared into
To the complex micro organism fungicide.
Embodiment nine
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 1 part of bacillus subtilis, 1 part of actinomyces, 5 parts of lactic acid bacterias, 2 parts of saccharomycete,
1 part of phosphate solubilizing bacteria and 1 part of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the bamboo handled by thermal cracking that parts by weight are 50 parts
It ferments in charcoal, the temperature of thermal cracking is 400 DEG C, and the temperature of fermented and cultured is 25 DEG C, and fermentation time 10h obtains thallus
Density is the fermenting agent of 103cfu/mL；
3 parts of fulvic acids, 10 parts of humic acid and 3 parts of bamboo acetic acids are mixed, 35min is stirred after disinfection, is added in fermenting agent
Stir 30h, and carry out two stages fermentation, obtain the complex micro organism fungicide, wherein two stages fermentation be divided into the first fermentation and
Second fermentation, the temperature of the first fermentation are 40 DEG C, and the time of the first fermentation is 3 days, and the temperature of the second fermentation is 50 DEG C, the second hair
The time of ferment is 3 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 40 DEG C, and the time of heat preservation is
2 days；
Composite fermentation microbial inoculum is dissolved in water, obtained lysate humidity is 55%, and drying, sorting, crushing are prepared into
To the complex micro organism fungicide.
Embodiment ten
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Microbial inoculum is weighed according to parts by weight, by 6 parts of bacillus subtilises, 6 parts of actinomyces, 15 parts of lactic acid bacterias, 8 parts of yeast
Bacterium, 6 parts of phosphate solubilizing bacterias and 6 parts of potassium solubilizing bacteria mixing, mixed microbial inoculum are added to the process thermal cracking that parts by weight are 100 parts and handle
Bamboo charcoal in ferment, the temperature of thermal cracking is 450 DEG C, and the temperature of fermented and cultured is 20 DEG C, and fermentation time 8h obtains bacterium
Volume density is the fermenting agent of 90cfu/mL；
15 parts of fulvic acids, 40 parts of humic acid and 15 parts of bamboo acetic acids are mixed, is added in fermenting agent after disinfection and stirs 50h,
And two stages fermentation is carried out, and the complex micro organism fungicide is obtained, wherein two stages fermentation is divided into the first fermentation and the second fermentation,
The temperature of first fermentation is 35 DEG C, and the time of the first fermentation is 2 days, and the temperature of the second fermentation is 40 DEG C, the time of the second fermentation
It is 2 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 40 DEG C, and the time of heat preservation is
1 day.
Embodiment 11
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 3 parts of bacillus subtilises, 3 parts of actinomyces, 8 parts of lactic acid bacterias, 4 parts of saccharomycete,
3 parts of phosphate solubilizing bacterias and 3 parts of potassium solubilizing bacteria mixing, it is 70 parts without thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of fermented and cultured is 30 DEG C, fermentation time 12h, obtains the hair that cell density is 110cfu/mL
Yeast-like fungi agent；
10 parts of fulvic acids and 26 parts of humic acid are mixed, is added in fermenting agent after disinfection and stirs 35h, and carry out two stages
Fermentation, obtains the complex micro organism fungicide, and wherein two stages fermentation is divided into the first fermentation and the second fermentation, the temperature of the first fermentation
Degree is 45 DEG C, and the time of the first fermentation is 4 days, and the temperature of the second fermentation is 52 DEG C, and the time of the second fermentation is 4 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 45 DEG C, and the time of heat preservation is
3 days.
Embodiment 12
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 3 parts of bacillus subtilises, 3 parts of actinomyces, 8 parts of lactic acid bacterias, 4 parts of saccharomycete,
3 parts of phosphate solubilizing bacterias and 3 parts of potassium solubilizing bacteria mixing, it is 70 parts without thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of fermented and cultured is 30 DEG C, fermentation time 12h, obtains the hair that cell density is 110cfu/mL
Yeast-like fungi agent；
15 parts of fulvic acids and 15 parts of humic acid are mixed, is added in fermenting agent after disinfection and stirs 35h, and carry out two stages
Fermentation, obtains the complex micro organism fungicide, and wherein two stages fermentation is divided into the first fermentation and the second fermentation, the temperature of the first fermentation
Degree is 45 DEG C, and the time of the first fermentation is 4 days, and the temperature of the second fermentation is 52 DEG C, and the time of the second fermentation is 4 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 45 DEG C, and the time of heat preservation is
3 days.
Embodiment 13
A kind of complex micro organism fungicide forms according to parts by weight, including：
The preparation method of the complex micro organism fungicide includes the following steps：
Weigh microbial inoculum according to parts by weight, by 3 parts of bacillus subtilises, 3 parts of actinomyces, 8 parts of lactic acid bacterias, 4 parts of saccharomycete,
3 parts of phosphate solubilizing bacterias and 3 parts of potassium solubilizing bacteria mixing, it is 70 parts without thermal cracking processing that mixed microbial inoculum, which is added to parts by weight,
It ferments in bamboo charcoal, the temperature of fermented and cultured is 30 DEG C, fermentation time 12h, obtains the hair that cell density is 110cfu/mL
Yeast-like fungi agent；
28 parts of humic acid and 8 parts of bamboo acetic acids are mixed, is added in fermenting agent after disinfection and stirs 35h, and carry out two stages
Fermentation, obtains the complex micro organism fungicide, and wherein two stages fermentation is divided into the first fermentation and the second fermentation, the temperature of the first fermentation
Degree is 45 DEG C, and the time of the first fermentation is 4 days, and the temperature of the second fermentation is 52 DEG C, and the time of the second fermentation is 4 days；
After first fermentation, incubation step is provided with before the second fermentation, and the temperature of heat preservation is 45 DEG C, and the time of heat preservation is
3 days.
Comparative example one
Comparative example one and embodiment six compare, and difference is only that not include potassium solubilizing bacteria, other conditions and six phase of embodiment
Together.
Comparative example two
Comparative example two and embodiment six compare, and difference is only that not include phosphate solubilizing bacteria, other conditions and six phase of embodiment
Together.
Comparative example three
Comparative example three and embodiment six compare, and difference is only that not include saccharomycete, other conditions and six phase of embodiment
Together.
Comparative example four
Comparative example four and embodiment six compare, and difference is only that not include lactic acid bacteria, other conditions and six phase of embodiment
Together.
Comparative example five
Comparative example five and embodiment six compare, and difference is only that not include actinomyces, other conditions and six phase of embodiment
Together.
Comparative example six
Comparative example six and embodiment six compare, and difference is only that not include bacillus subtilis, other conditions and implementation
Example six is identical.
Comparative example seven
Comparative example seven and embodiment six compare, and difference, which is only that, replaces bamboo charcoal with the wheat bran of same parts by weight, other
Condition is identical as embodiment six.
Comparative example eight
Comparative example eight and embodiment six compare, and difference, which is only that, replaces bamboo charcoal with the stalk of same parts by weight, other
Condition is identical as embodiment six.
The face of the complex micro organism fungicide of complex micro organism fungicide and comparative example preparation in embodiment provided by the invention
Color is as shown in table 1 in 15 days, 25 days and 35 days comparative situations：
Table 1
As it can be seen from table 1 comparing embodiment one and embodiment two it is found that bamboo is hot cracked after obtained bamboo charcoal can
Shorten the fermentation time of complex micro organism fungicide, improves fermentation efficiency；Embodiment two is compared with embodiment eight it is found that addition auxiliary material
The fermentation time of complex micro organism fungicide can be shortened, improve fermentation efficiency；
Embodiment 11, embodiment 12 and embodiment 13 and embodiment six be compared it is found that in auxiliary material three kinds at
Dividing mutually to cooperate with, and shorten the fermentation time of complex micro organism fungicide, promote the fermentation of bamboo charcoal, improve fermentation efficiency；Comparison
Example one, comparative example two, comparative example three, comparative example four, comparative example five and comparative example six are compared with embodiment six it is found that composite microbial
Mutual collaboration in object microbial inoculum between bacillus subtilis, actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria and potassium solubilizing bacteria is being shortened
Fermentation time plays a major role in terms of the fermentation for promoting bamboo charcoal, and the product of fermentation is with the extension of time, occur apparent point
Change；It is found that having used different carbon sources compared with embodiment six, bamboo charcoal more can than wheat bran, stalk etc. for comparative example seven, comparative example eight
It interacts with bacillus subtilis, actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria and potassium solubilizing bacteria, and it is provided by the invention
Within the scope of experiment parameter of the invention, product shows consistent as a result, product the complex micro organism fungicide of method preparation
Stable feature is shown, illustrating method of the invention has stronger stability and practicability.
The hand of the complex micro organism fungicide of complex micro organism fungicide and comparative example preparation in embodiment provided by the invention
Sense is as shown in table 2 in 15 days, 25 days and 35 days comparative situations：
Table 2
From table 2 it can be seen that comparing embodiment one and embodiment two it is found that bamboo is hot cracked after obtained bamboo charcoal can
Shorten the fermentation time of complex micro organism fungicide, improves fermentation efficiency；Embodiment two is compared with embodiment eight it is found that addition auxiliary material
The fermentation time of complex micro organism fungicide can be shortened, improve fermentation efficiency；
Embodiment 11, embodiment 12 and embodiment 13 and embodiment six be compared it is found that in auxiliary material three kinds at
Dividing mutually to cooperate with, and shorten the fermentation time of complex micro organism fungicide, promote the fermentation of bamboo charcoal, improve fermentation efficiency；Comparison
Example one, comparative example two, comparative example three, comparative example four, comparative example five and comparative example six are compared with embodiment six it is found that composite microbial
Mutual collaboration in object microbial inoculum between bacillus subtilis, actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria and potassium solubilizing bacteria is being shortened
Fermentation time plays a major role in terms of the fermentation for promoting bamboo charcoal；Comparative example seven, comparative example eight are compared with embodiment six it is found that bamboo
Charcoal can more occur mutual than the wheat head, stalk etc. with bacillus subtilis, actinomyces, lactic acid bacteria, saccharomycete, phosphate solubilizing bacteria and potassium solubilizing bacteria
Effect.The complex micro organism fungicide of preparation method preparation provided in an embodiment of the present invention, property is preferable, further convenient for the later period
Using and materials.
The gas of the complex micro organism fungicide of complex micro organism fungicide and comparative example preparation in embodiment provided by the invention
Taste is as shown in table 3 in 15 days, 25 days and 35 days comparative situations：
Table 3
From table 3 it can be seen that comparing embodiment two and embodiment eight are it is found that addition auxiliary material can improve composite fermentation microbial inoculum
Smell when fermentation；Embodiment 11 is respectively it is found that the addition of bamboo acetic acid can compared with embodiment 12 and embodiment 13
Improve the smell when fermentation of composite fermentation microbial inoculum.As it can be seen that the complex micro organism fungicide of present invention method preparation, substantially
There is no peculiar smell, is a kind of environmentally friendly complex micro organism fungicide.
In conclusion complex micro organism fungicide provided by the invention working in coordination by each strain, it can to bamboo charcoal
It is quickly decomposed, crumb structure can be improved to soil, excellent micro-ecological environment is capable of forming to root system of plant, realizes microbial inoculum
Multifunctional comprehensive synergy target；Cooperate by using composite bacteria agent with the bamboo charcoal and auxiliary material specifically handled in the present invention,
It can delay the release in the sub- soil of fertilizer nutrient, while can significantly improve soil texture, increase effective N, P, K group in soil
Point, increase the nutrient of soil, shortens fermentation time, promote the fermentation of bamboo charcoal；Complex micro organism fungicide of the present invention, formula is simple,
Ferment effect is good, and preparation method is simple, and operating process is easy, and preparation condition is mild, easy to accomplish and control, be easy to largely into
Row preparation, is conducive to industrial application；Complex micro organism fungicide provided by the invention is used to prepare feed and/or feed supplementation.
Note that the above is only a better embodiment of the present invention and the applied technical principle.It will be appreciated by those skilled in the art that
The invention is not limited to the specific embodiments described herein, be able to carry out for a person skilled in the art it is various it is apparent variation,
It readjusts and substitutes without departing from protection scope of the present invention.Therefore, although being carried out by above embodiments to the present invention
It is described in further detail, but the present invention is not limited to the above embodiments only, without departing from the inventive concept, also
It may include more other equivalent embodiments, and the scope of the invention is determined by the scope of the appended claims.
Claims (10)
1. a kind of complex micro organism fungicide for bamboo charcoal fermentation, which is characterized in that the complex micro organism fungicide is by weight
Number includes：1-6 parts of bacillus subtilis, 1-6 parts of actinomyces, 5-15 parts of lactic acid bacteria, 2-8 parts of saccharomycete, 1-6 parts of phosphate solubilizing bacteria reconciliation
1-6 parts of potassium bacterium.
2. complex micro organism fungicide according to claim 1, which is characterized in that the complex micro organism fungicide is by weight
Number includes：1-3 parts of bacillus subtilis, 1-3 parts of actinomyces, 6-10 parts of lactic acid bacteria, 3-6 parts of saccharomycete, 1-3 parts of phosphate solubilizing bacteria reconciliation
1-3 parts of potassium bacterium.
3. complex micro organism fungicide according to claim 1 or 2, which is characterized in that the complex micro organism fungicide also wraps
Include auxiliary material；
Preferably, the auxiliary material be fulvic acid, humic acid or bamboo acetic acid in any one or at least two combination, preferably
The combination of fulvic acid, humic acid and bamboo acetic acid；
Preferably, the fulvic acid accounts for 3-15 part of the complex micro organism fungicide in parts by weight, and preferably 5-10 parts；
Preferably, the humic acid accounts for 10-40 part of the complex micro organism fungicide in parts by weight, and preferably 15-30 parts；
Preferably, the bamboo acetic acid accounts for 3-15 part of the complex micro organism fungicide in parts by weight, and preferably 6-10 parts.
4. the fertilizer that a kind of complex biological microbial inoculum as claimed in any one of claims 1-3 is used to prepare bamboo charcoal fermentation；
Preferably, the bamboo charcoal accounts for 50-100 part of the fertilizer in parts by weight, and preferably 60-85 parts；
Preferably, the fertilizer includes the following raw material in parts by weight：1-6 parts of bacillus subtilis, 1-6 parts of actinomyces, lactic acid
5-15 parts of bacterium, 2-8 parts of saccharomycete, 1-6 parts of phosphate solubilizing bacteria, 1-6 parts of potassium solubilizing bacteria, 50-100 parts of bamboo charcoal, 3-15 parts of fulvic acid, humic acid
10-40 parts and bamboo acetic acid 3-15 parts；
Preferably, the fertilizer includes the following raw material in parts by weight：1-3 parts of bacillus subtilis, 1-3 parts of actinomyces, lactic acid
6-10 parts of bacterium, 3-6 parts of saccharomycete, 1-3 parts of phosphate solubilizing bacteria, 1-3 parts of potassium solubilizing bacteria, 60-85 parts of bamboo charcoal, 5-10 parts of fulvic acid, humic acid
15-30 parts and bamboo acetic acid 6-10 parts.
5. a kind of preparation method of fertilizer as claimed in claim 4, which is characterized in that include the following steps：
(1) bacterium solution is carried out by formula ratio respectively carrying out cultivation and fermentation in mixing addition bamboo charcoal, obtains fermenting agent；
(2) raw material of fertilizer is added in the fermentation liquid of step (1) by formula ratio and stirs and carry out two stages fermentation, obtained described
Fertilizer.
6. preparation method according to claim 5, which is characterized in that the temperature of step (1) described fermented and cultured is 20-35
DEG C, preferably 25-32 DEG C；
Preferably, the time of step (1) described fermentation is 8-18h, preferably 10-14h；
Preferably, the cell density of fermenting agent obtained by step (1) is 90-120cfu/mL, preferably 103-118cfu/mL；
Preferably, the time of the stirring is 20-50h, preferably 30-40h.
7. preparation method according to claim 5 or 6, which is characterized in that first in the two stages fermentation, which ferments, is
Aerobic fermentation；
Preferably, the temperature of first fermentation is 35-60 DEG C, preferably 40-52 DEG C；
Preferably, the time of first fermentation is 2-6 days, preferably 3-5 days；
Preferably, further include the steps that heat preservation after first fermentation；
Preferably, the temperature of the heat preservation is 40-60 DEG C, preferably 40-52 DEG C；
Preferably, the time of the heat preservation is 1-5 days, preferably 2-3 days；
Preferably, the second fermentation in the two stages fermentation is anaerobic fermentation；
Preferably, the temperature of second fermentation is 40-65 DEG C, preferably 50-59 DEG C；
Preferably, the time of second fermentation is 2-6 days, preferably 3-5 days.
8. the preparation method according to any one of claim 5-7, which is characterized in that further include bamboo charcoal before step (1)
Pretreated step；
Preferably, the pretreatment obtains the bamboo charcoal for bamboo is carried out thermal cracking；
Preferably, the temperature of the thermal cracking is 300-500 DEG C, preferably 350-450 DEG C.
9. the preparation method according to any one of claim 5-8, which is characterized in that include the following steps：
It (1) is that 300-500 DEG C of thermal cracking high mountain moso bamboo obtains bamboo charcoal in temperature；
(2) bacterium solution is carried out mixing by formula ratio respectively to be added in bamboo charcoal in 20-35 DEG C of temperature progress cultivation and fermentation 8-18h, is obtained
To fermenting agent, cell density 90-120cfu/mL；
(3) auxiliary material is mixed and is sterilized, stir 30-40min, and be added in the fermentation liquid of step (1) by formula ratio and stir 20-50h,
And two stages fermentation is carried out, and tunning is dissolved in water, the humidity of obtained lysate is 40-55%, dry, sort,
The complex micro organism fungicide is prepared in crushing；
Wherein, two stages fermentation is divided into the first fermentation and the second fermentation, and the temperature of the first fermentation is 35-60 DEG C, time 2-6
It, keeps the temperature 1-5 days, and the temperature of the second fermentation is 40-65 DEG C, and the time is 2-6 days.
10. a kind of fertilizer as claimed in claim 4 is used to prepare agricultural composite fertilizer.
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