CN108892591A - A kind of natural environmental-protective farmyard manure, preparation method and its application in pepper planting - Google Patents
A kind of natural environmental-protective farmyard manure, preparation method and its application in pepper planting Download PDFInfo
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- CN108892591A CN108892591A CN201810987654.6A CN201810987654A CN108892591A CN 108892591 A CN108892591 A CN 108892591A CN 201810987654 A CN201810987654 A CN 201810987654A CN 108892591 A CN108892591 A CN 108892591A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
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- Environmental Sciences (AREA)
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Abstract
The invention discloses a kind of natural environmental-protective farmyard manure, preparation method and its applications in pepper planting.The natural environmental-protective farmyard manure includes fertilizer kernel, inner investment layer and outer coating layer.Natural environmental-protective farmyard manure of the present invention is a kind of good bio-organic fertilizer, a great number of elements and the microelements such as nitrogen rich in, phosphorus, potassium, and plant growth substance and humic acid etc., compared with chemical fertilizer, it is degradable, nontoxic, pollution-free, to person poultry harmless, soil hardening can be caused to avoid chronic administration chemical fertilizer, soil fertility can be increased, improve soil texture, soil moisture is kept, there is significant impact to composite factors such as plant height, the yield and qualities of capsicum, can be promoted and applied in pepper planting.
Description
Technical field
The present invention relates to for the new-type fertilizer of high-quality and efficient safety in production, pesticide, soil improvement material and plant growth
Regulator production technical field, more particularly to a kind of natural environmental-protective farmyard manure, preparation method and its answering in pepper planting
With.
Background technique
Sufficient moisture and nutrient supply is two primary conditions for guaranteeing crops normal growth.The 1960s with
Come, have benefited from a large amount of investments of pesticide, water and fertilizer in intensive agricultural, world food yield is skyrocketed through, and starvation is to the whole world
Population increases bring threat and is gradually reduced.It can be by crops however, being applied to fertilizer only a small part in farmland
It is absorbed and utilized, most of nutrient can be escaped into atmosphere by volatilization after application or be permeated the ground in water by leaching
It is constantly lost, serious environmental pollution is caused, such as water eutrophication, acid rain and Global Greenhouse Effect.In addition, fertilizer loss is not
It is relatively low to the utilization rate of nutrient to only result in crops, also creates serious economic loss and the wasting of resources.
Chemical fertilizer is in modern agricultural production in occupation of very important status.Plantation for capsicum, it is necessary to which there have to be suitable
Fertilizer supply, and scientific and reasonable application is just conducive to the growth of capsicum and the raising of yield.According to statistics, fertilizer increases capsicum
Production has very important effect, and the rational application of fertilizer can not only make yield of hot pepper be improved significantly, and can also reduce disease pest
Harmful generation improves the quality of capsicum.And balance fertilizing has very important meaning to the problem in pepper planting is solved
Justice.
It is detected by the soil fertility to pepper planting garden, finds contribution of the current capsicum fertilizer to capsicum product
Rate is very low, but the yield of China's chemical fertilizer and usage amount occupy the first in the world.Currently, vegetable melon and fruit and the various chemical fertilizer of crops
Utilization rate there was only about 32%, the only half of developed country.This brings very big bear to the financial resources and environment in China
Face is rung.Chemical fertilizer have the characteristics that easily use, it is quick, important facilitation is played to the growth of yield of hot pepper, for a long time
Using make green pepper agricultures form kind of capsicum will apply fertilizer more just have fecund go out idea.
Then the phenomenon that being formed the overfertilization in pepper planting management, but not with the promotion of dose
The corresponding growth effect of the yield envisioned, quality is same, this more fertilising for not meeting peasant is recognized with regard to what fecund went out
Know.And upper substantial contribution is wasted on fertilizer, yield fails to be correspondinglyd increase, and has instead resulted in reduction and the environment of yield
Pollution.Therefore, the utilization rate for how improving fertilizer needed for pepper planting is an important factor for influencing capsicum output.
Summary of the invention
The first object of the present invention is to provide a kind of natural environmental-protective farmyard manure.
The present invention discloses a kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The second object of the present invention is to provide a kind of preparation method of natural environmental-protective farmyard manure.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：2~3g of konjak glucomannan and 0.2~1g of ammonium polyphosphate are uniformly mixed, mixture is obtained；It will
Mixture is added in 30~80g of granulated fertilizer, stirs 10~30 minutes, so that mixture is evenly coated at granulated fertilizer surface；
It is dry, obtain fertilizer kernel；
Step 2：1~2g of konjak glucomannan, 0.3~0.6g of sodium carbonate and 100~180 μ L of polyethylene glycol-400 is molten
Solution stirs 30~50 minutes in 100~200mL water, obtains the konjak glucomannan solution of deacetylation；By deacetylation
Konjak glucomannan solution and the obtained fertilizer kernel of step 1 be uniformly mixed, stir 10~20 minutes, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is dry, inner investment layer is formed in fertilizer core surface, is obtained containing in
The fertilizer of coating layer；
Step 3：The fertilizer containing inner investment layer that 0.3~1g of water-retaining agent and step 2 are obtained is uniformly mixed, and stirring 5~
10 minutes, so that water-retaining agent is evenly coated at the surface of inner investment layer；It is dry, outer coating layer is formed on the surface of interior coating layer,
Obtain the natural environmental-protective farmyard manure.
In some technical solutions of the invention, the granulated fertilizer is granular urea.
In some technical solutions of the invention, the granulated fertilizer obtains by the following method：Particulate vector is sterilized；
By after sterilizing particulate vector and liquid bacterial fertilizer with 1:(0.8~1.5) (g/mL) is uniformly mixed, and is subsequently placed in 28~30 DEG C of placements
7~10 days, obtain the granulated fertilizer；Preferably, the particulate vector is charcoal and/or corn cob granule.
In some technical solutions of the invention, the granulated fertilizer obtains by the following method：Particulate vector is sterilized；
By after sterilizing particulate vector, ferrihydrite or modified ferrihydrite and liquid bacterial fertilizer with 1：(0.0001~0.0003)：(0.8~
1.5) (g/g/mL) is uniformly mixed, and is subsequently placed in 28~30 DEG C and is placed 7~10 days, obtains the granulated fertilizer；Preferably, described
Particulate vector is charcoal and/or corn cob granule.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：(0.2~0.3) (g/mL) plus water,
Grinding 5~10 minutes obtains seaweed homogenate；Adjust seaweed homogenate pH to 6~7, according to seaweed homogenate quality 0.1%~
0.3% is added complex enzyme, 40~50 DEG C enzyme digestion reaction 48~72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzymatic hydrolysis
After reaction seaweed homogenate, add enzyme digestion reaction after seaweed homogenate quality 0.15%~0.2% function microbial inoculum, adjust pH to
6~7, it ferments 24~48 hours in 35~40 DEG C；It is centrifuged 10~20 minutes with 3000~4000 revs/min later, collects supernatant
Liquid obtains the liquid bacterial fertilizer.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
The preparation process of the ferrihydrite is：Prepare the FeCl of 0.3~0.5mol/L3·6H2O solution and 0.5~
The NaOH aqueous solution of 1.5mol/L；Under agitation, NaOH aqueous solution is added to FeCl3·6H2In O solution, supervised with pH meter
PH variation in solution is surveyed, until pH reaches 6~7, forms suspension；Then suspension is stood 2~6 hours makes its aging, obtains
Suspension after to aging；Suspension after aging is centrifuged 20~30 minutes in 2~4 DEG C, sediment is collected, obtains the water
Iron ore.
In the preparation process of ferrihydrite, using the superior adsorption function of ferrihydrite by itself and urea combination, water iron is utilized
Mine is combined as carrier and nitrogen, promotes plant to Nitrogen Absorption；Nutrient is utilized urea as again promotes plant growth, from
And promote plant that iron is absorbed and utilized.
The preparation process of the modified ferrihydrite is：The raw material of iron reduction culture medium is uniformly mixed according to formula, configuration
The iron reduction culture medium is obtained, wherein the formula of iron reduction culture medium is：Na2HPO4·7H212~13g/L of O,
KH2PO4·7H2O 2~4g/L, NH41~2g/L of Cl, 2~3g/L of yeast extract, surplus are water；Then every liter of iron also
3~4g of lactic acid is added in former culture medium, as the electron donor of ferriferous oxide, sterilizes 20~30 minutes, obtains in 120~121 DEG C
Iron after sterilizing restores culture medium；Then ferrihydrite is added to the iron reduction culture medium after sterilizing, makes ferro element in culture medium
Concentration is 60~80mg/L；Be eventually adding according to volume ratio 2~3% be inoculated with iron-reducing bacterium, in 28~30 DEG C of Anaerobic culturels 10~
15 days, collect Anaerobic culturel product；Anaerobic culturel product is centrifuged 20~30 minutes, bottom precipitation is collected；Bottom precipitation is used
Deionized water washing, it is dry, obtain the modified ferrihydrite.
The iron-reducing bacteria is Shewanella and/or lysine bacillus.
The water-retaining agent is the compound of one or more of polyacrylamide, polysuccinimide, poly-aspartate.
Preferably, the water-retaining agent is polysuccinimide and polyacrylamide with mass ratio 1：The mixture of 1 composition.
The third object of the present invention is to disclose application of the natural environmental-protective farmyard manure in pepper planting.
Natural environmental-protective farmyard manure of the present invention is a kind of good bio-organic fertilizer, nitrogen rich in, phosphorus, potassium etc.
A great number of elements and microelement and plant growth substance and humic acid etc., compared with chemical fertilizer, degradable, nontoxic, nothing
Pollution, to person poultry harmless, can cause soil hardening to avoid chronic administration chemical fertilizer, can increase soil fertility, improve soil
Structure keeps soil moisture, has significant impact to plant height, the yield and quality composite factor of capsicum, can be in capsicum kind
It is promoted and applied in plant.
Specific embodiment
Raw material is described below in embodiment：
Konjak glucomannan, No. CAS：37220-17-0 is specifically provided by Nanjing Song Guan Biotechnology Co., Ltd, packet
Fill specification 25kg.
Ammonium polyphosphate, No. CAS：68333-79-9 is specifically provided by Hubei Jusheng Science and Technology Ltd., number 269-
789-9。
Granular urea, Zouping good luck Biotechnology Co., Ltd provide, and partial size is 1.0~1.3mm.
Corn cob granule, Lingshou County sweet-smelling mineral products processing factory provide.
Seaweed specifically uses kelp.
Cellulase, the Nanning road Dong Henghua biotechnology Co., Ltd provide, and enzyme activity is 10,000 U/g.
Pectase, the Nanning road Dong Henghua biotechnology Co., Ltd provide, and enzyme activity is 10,000 U/g.
Protease specifically uses bromelain, and Shandong Ju Rong bioengineering Co., Ltd provides, 100,000 U/g of enzyme activity.
Bacillus subtilis, Jinan sapphire member new material Co., Ltd provide, and viable bacteria concentration is 100,000,000,000/mL.
Bacillus licheniformis, Jinan sapphire member new material Co., Ltd provide, and viable bacteria concentration is 100,000,000,000/mL.
Lactic acid bacteria, Cangzhou Xin great Di Biotechnology Co., Ltd provide, and viable bacteria concentration is 5,000,000,000/mL.
Saccharomycete, Yangzhou Haicheng Bioisystech Co., Ltd provide, and viable bacteria concentration is 5,000,000,000/mL.
Polysuccinimide, No. CAS：5608-40-6 is specifically provided by Xi'an Jia Boying Biotechnology Co., Ltd, point
Son amount 7000.
Polyacrylamide, No. CAS：9003-05-8 is specifically provided by Henan all directions water-purifying material Co., Ltd, molecular weight
800-1000 ten thousand.
Lysine bacillus, Shanghai Ke Wei chemical technology Co., Ltd provide, ATCC4525,3,000,000,000/mL of viable bacteria concentration.
Embodiment 1
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granular urea 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granular urea surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that polysuccinimide 1g and step 2 are obtained is uniformly mixed, with 100
Rev/min stirring 5 minutes so that water-retaining agent is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, in inner investment
The surface of layer forms outer coating layer, obtains the natural environmental-protective farmyard manure.
Embodiment 2
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granulated fertilizer 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granulated fertilizer surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that polysuccinimide 1g and step 2 are obtained is uniformly mixed, with 100
Rev/min stirring 5 minutes so that water-retaining agent is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, in inner investment
The surface of layer forms outer coating layer, obtains the natural environmental-protective farmyard manure.
The granulated fertilizer obtains by the following method：Corn cob granule is sterilized 2 hours in 160 DEG C；After sterilizing
Particulate vector and liquid bacterial fertilizer are with 1：1 (g/mL) is uniformly mixed, and is subsequently placed in 30 DEG C and is placed 7 days, obtains the granulated fertilizer.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：0.3 (g/mL) plus water, with 4000
Rev/min grinding 5 minutes, obtain seaweed homogenate；The pH to 6 that seaweed homogenate is adjusted with the hydrochloric acid of mass fraction 1%, according to seaweed
Complex enzyme is added in be homogenized quality 0.3%, 50 DEG C enzyme digestion reaction 72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzyme
Seaweed homogenate after solution reaction, the function microbial inoculum of the seaweed homogenate quality 0.2% after adding enzyme digestion reaction, using mass fraction
1% sodium hydrate aqueous solution adjusts pH to 7, ferments 48 hours at 35 DEG C；It is centrifuged 10 minutes with 4000 revs/min later,
Supernatant is collected, the liquid bacterial fertilizer is obtained.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
Embodiment 3
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granulated fertilizer 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granulated fertilizer surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that polysuccinimide 1g and step 2 are obtained is uniformly mixed, with 100
Rev/min stirring 5 minutes so that water-retaining agent is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, in inner investment
The surface of layer forms outer coating layer, obtains the natural environmental-protective farmyard manure.
The granulated fertilizer obtains by the following method：Corn cob granule is sterilized 2 hours in 160 DEG C；After sterilizing
Particulate vector, ferrihydrite and liquid bacterial fertilizer are with 1：0.03%：1 (g/g/mL) is uniformly mixed, and is subsequently placed in 30 DEG C and is placed 7 days, obtains
To the granulated fertilizer.
The preparation process of the ferrihydrite is：Prepare the FeCl of 0.4mol/L3·6H2The NaOH of O solution and 1mol/L are water-soluble
Liquid；Under 100 revs/min of stirring condition, NaOH aqueous solution is added to FeCl3·6H2In O solution, solution is monitored with pH meter
Middle pH variation forms suspension until pH reaches 7；Then suspension is stood 4 hours makes its aging, outstanding after obtaining aging
Turbid；Suspension after aging is centrifuged 20 minutes in 4 DEG C with 3000 revs/min, sediment is collected, obtains the ferrihydrite.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：0.3 (g/mL) plus water, with 4000
Rev/min grinding 5 minutes, obtain seaweed homogenate；The pH to 6 that seaweed homogenate is adjusted with the hydrochloric acid of mass fraction 1%, according to seaweed
Complex enzyme is added in be homogenized quality 0.3%, 50 DEG C enzyme digestion reaction 72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzyme
Seaweed homogenate after solution reaction, the function microbial inoculum of the seaweed homogenate quality 0.2% after adding enzyme digestion reaction, using mass fraction
1% sodium hydrate aqueous solution adjusts pH to 7, ferments 48 hours at 35 DEG C；It is centrifuged 10 minutes with 4000 revs/min later,
Supernatant is collected, the liquid bacterial fertilizer is obtained.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
Embodiment 4
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granulated fertilizer 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granulated fertilizer surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that polysuccinimide 1g and step 2 are obtained is uniformly mixed, with 100
Rev/min stirring 5 minutes so that water-retaining agent is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, in inner investment
The surface of layer forms outer coating layer, obtains the natural environmental-protective farmyard manure.
The granulated fertilizer obtains by the following method：Corn cob granule is sterilized 2 hours in 160 DEG C；After sterilizing
Particulate vector, modified ferrihydrite and liquid bacterial fertilizer are with 1：0.03%：1 (g/g/mL) is uniformly mixed, and is subsequently placed in 30 DEG C and is placed 7
It, obtains the granulated fertilizer.
The preparation process of the modified ferrihydrite is：The raw material of iron reduction culture medium is uniformly mixed according to formula, configuration
The iron reduction culture medium is obtained, wherein the formula of iron reduction culture medium is：Na2HPO4·7H2O 12.8g/L,
KH2PO4·7H2O 3g/L, NH4Cl 1g/L, yeast extract 2g/L, surplus are water；Then culture medium is being restored in every liter of iron
Lactic acid 3g is added, as the electron donor of ferriferous oxide, sterilizes 20 minutes in 121 DEG C, the iron after being sterilized restores culture medium；
Then ferrihydrite is added to the iron reduction culture medium after sterilizing, makes the concentration 70mg/L of ferro element in culture medium；It is eventually adding
According to volume ratio 2% be inoculated with lysine bacillus, in 30 DEG C Anaerobic culturel 15 days, collect Anaerobic culturel product；Anaerobism is trained
It supports product to be centrifuged 20 minutes with 5000 revs/min, collects bottom precipitation；By 300 times of its quality of deionized water of bottom precipitation
Washing, it is dry to constant weight in 60 DEG C, obtain the modified ferrihydrite.
The preparation of the ferrihydrite is the same as embodiment 3.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：0.3 (g/mL) plus water, with 4000
Rev/min grinding 5 minutes, obtain seaweed homogenate；The pH to 6 that seaweed homogenate is adjusted with the hydrochloric acid of mass fraction 1%, according to seaweed
Complex enzyme is added in be homogenized quality 0.3%, 50 DEG C enzyme digestion reaction 72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzyme
Seaweed homogenate after solution reaction, the function microbial inoculum of the seaweed homogenate quality 0.2% after adding enzyme digestion reaction, using mass fraction
1% sodium hydrate aqueous solution adjusts pH to 7, ferments 48 hours at 35 DEG C；It is centrifuged 10 minutes with 4000 revs/min later,
Supernatant is collected, the liquid bacterial fertilizer is obtained.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
Embodiment 5
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granulated fertilizer 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granulated fertilizer surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that polyacrylamide 1g and step 2 are obtained is uniformly mixed, with 100 turns/
Minute stirring 5 minutes, so that water-retaining agent is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, in interior coating layer
Surface formed outer coating layer, obtain the natural environmental-protective farmyard manure.
The granulated fertilizer obtains by the following method：Corn cob granule is sterilized 2 hours in 160 DEG C；After sterilizing
Particulate vector, modified ferrihydrite and liquid bacterial fertilizer are with 1：0.03%：1 (g/g/mL) is uniformly mixed, and is subsequently placed in 30 DEG C and is placed 7
It, obtains the granulated fertilizer.
The preparation process of the modified ferrihydrite is：The raw material of iron reduction culture medium is uniformly mixed according to formula, configuration
The iron reduction culture medium is obtained, wherein the formula of iron reduction culture medium is：Na2HPO4·7H2O 12.8g/L,
KH2PO4·7H2O 3g/L, NH4Cl 1g/L, yeast extract 2g/L, surplus are water；Then culture medium is being restored in every liter of iron
Lactic acid 3g is added, as the electron donor of ferriferous oxide, sterilizes 20 minutes in 121 DEG C, the iron after being sterilized restores culture medium；
Then ferrihydrite is added to the iron reduction culture medium after sterilizing, makes the concentration 70mg/L of ferro element in culture medium；It is eventually adding
According to volume ratio 2% be inoculated with lysine bacillus, in 30 DEG C Anaerobic culturel 15 days, collect Anaerobic culturel product；Anaerobism is trained
It supports product to be centrifuged 20 minutes with 5000 revs/min, collects bottom precipitation；By 300 times of its quality of deionized water of bottom precipitation
Washing, it is dry to constant weight in 60 DEG C, obtain the modified ferrihydrite.
The preparation of the ferrihydrite is the same as embodiment 3.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：0.3 (g/mL) plus water, with 4000
Rev/min grinding 5 minutes, obtain seaweed homogenate；The pH to 6 that seaweed homogenate is adjusted with the hydrochloric acid of mass fraction 1%, according to seaweed
Complex enzyme is added in be homogenized quality 0.3%, 50 DEG C enzyme digestion reaction 72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzyme
Seaweed homogenate after solution reaction, the function microbial inoculum of the seaweed homogenate quality 0.2% after adding enzyme digestion reaction, using mass fraction
1% sodium hydrate aqueous solution adjusts pH to 7, ferments 48 hours at 35 DEG C；It is centrifuged 10 minutes with 4000 revs/min later,
Supernatant is collected, the liquid bacterial fertilizer is obtained.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
Embodiment 6
A kind of natural environmental-protective farmyard manure, including fertilizer kernel, inner investment layer and outer coating layer.
The preparation method of the natural environmental-protective farmyard manure, including following three step：
Step 1：Konjak glucomannan 2g and ammonium polyphosphate 1g are uniformly mixed, mixture is obtained；By mixture plus
Enter in granulated fertilizer 80g, is stirred 30 minutes with 100 revs/min, so that mixture is evenly coated at granulated fertilizer surface；In 30
DEG C dry 12 hours, obtain fertilizer kernel；
Step 2：Konjak glucomannan 1g, sodium carbonate 0.4g and 160 μ L of polyethylene glycol-400 are dissolved in 100mL water
In, it is stirred 40 minutes with 100 revs/min, obtains the konjak glucomannan solution of deacetylation；By the konjaku Portugal of deacetylation
The fertilizer kernel that mannan solution and step 1 obtain is uniformly mixed, and is stirred 20 minutes with 100 revs/min, so that Amorphophallus rivieri glucomannan
Dew glycan solution is evenly coated at the surface of fertilizer kernel；It is 12 hours dry in 30 DEG C, inner investment is formed in fertilizer core surface
Layer, obtains the fertilizer containing inner investment layer；
Step 3：The fertilizer containing inner investment layer that water-retaining agent 1g and step 2 are obtained is uniformly mixed, and the water-retaining agent is
Polysuccinimide and polyacrylamide are with mass ratio 1：The mixture of 1 composition, is stirred 5 minutes with 100 revs/min, so that protecting
Aqua is evenly coated at the surface of inner investment layer；It is 12 hours dry in 30 DEG C, outer coating layer is formed on the surface of interior coating layer, is obtained
To the natural environmental-protective farmyard manure.
The granulated fertilizer obtains by the following method：Corn cob granule is sterilized 2 hours in 160 DEG C；After sterilizing
Particulate vector, modified ferrihydrite and liquid bacterial fertilizer are with 1：0.03%：1 (g/g/mL) is uniformly mixed, and is subsequently placed in 30 DEG C and is placed 7
It, obtains the granulated fertilizer.
The preparation process of the modified ferrihydrite is：The raw material of iron reduction culture medium is uniformly mixed according to formula, configuration
The iron reduction culture medium is obtained, wherein the formula of iron reduction culture medium is：Na2HPO4·7H2O 12.8g/L,
KH2PO4·7H2O 3g/L, NH4Cl 1g/L, yeast extract 2g/L, surplus are water；Then culture medium is being restored in every liter of iron
Lactic acid 3g is added, as the electron donor of ferriferous oxide, sterilizes 20 minutes in 121 DEG C, the iron after being sterilized restores culture medium；
Then ferrihydrite is added to the iron reduction culture medium after sterilizing, makes the concentration 70mg/L of ferro element in culture medium；It is eventually adding
According to volume ratio 2% be inoculated with lysine bacillus, in 30 DEG C Anaerobic culturel 15 days, collect Anaerobic culturel product；Anaerobism is trained
It supports product to be centrifuged 20 minutes with 5000 revs/min, collects bottom precipitation；By 300 times of its quality of deionized water of bottom precipitation
Washing, it is dry to constant weight in 60 DEG C, obtain the modified ferrihydrite.
The preparation of the ferrihydrite is the same as embodiment 3.
The preparation process of the liquid bacterial fertilizer is：New fresh seaweed is taken, according to solid-liquid ratio 1：0.3 (g/mL) plus water, with 4000
Rev/min grinding 5 minutes, obtain seaweed homogenate；The pH to 6 that seaweed homogenate is adjusted with the hydrochloric acid of mass fraction 1%, according to seaweed
Complex enzyme is added in be homogenized quality 0.3%, 50 DEG C enzyme digestion reaction 72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Take enzyme
Seaweed homogenate after solution reaction, the function microbial inoculum of the seaweed homogenate quality 0.2% after adding enzyme digestion reaction, using mass fraction
1% sodium hydrate aqueous solution adjusts pH to 7, ferments 48 hours at 35 DEG C；It is centrifuged 10 minutes with 4000 revs/min later,
Supernatant is collected, the liquid bacterial fertilizer is obtained.
The complex enzyme is by cellulase, pectase and protease with mass ratio 2：l：1 composition.
The function microbial inoculum obtains by the following method：Bacillus subtilis is seeded to Liquid Culture with volume ratio 2%
(group becomes base A：1wt% tryptone, 0.5wt% yeast extract, 1wt% sodium chloride, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution A is obtained；Lactic acid bacteria with volume ratio 2% is seeded to fluid nutrient medium B, and (group becomes：
1wt% beef extract, 1wt% peptone, 0.5wt% yeast extract, 2wt% glucose, 0.5wt% sodium acetate, 0.2wt% phosphoric acid hydrogen
Dipotassium, 0.2wt% Triammonium citrate, 0.01wt% Tween-80,0.058wt% epsom salt, tetra- water sulfuric acid of 0.025wt%
Manganese, surplus are water) in, it is cultivated 18 hours in 37 DEG C with 200 revs/min, obtains bacterium solution B；Saccharomycete is inoculated with volume ratio 2%
To fluid nutrient medium C, (group becomes：1wt% yeast extract, 2wt% peptone, 2wt% glucose, surplus are water) in, in 37 DEG C with
200 revs/min are cultivated 18 hours, and bacterium solution C is obtained；By bacterium solution A, bacterium solution B and bacterium solution C with volume ratio 1：1：1 mixing obtains described
Function microbial inoculum.
Pepper planting field trial is carried out to the natural environmental-protective farmyard manure that Examples 1 to 6 obtains.
Field trial place is for the specific nutrient situation for trying soil in Hunan Province, field trial：PH value 4.35, soil has
Machine matter measured value 2.35%, active acid 1.6mg/L, calcium 350mg/L, magnesium 220mg/L, potassium 86mg/L, ammonium ion 29mg/L, phosphorus
21mg/L, sulphur 74mg/L, boron 2.5mg/L, copper 3.8mg/L, iron 80mg/L, manganese 31mg/L, zinc 4.8mg/L.
Capsicum variety is Hunan native breed, emerging vegetable 215.
The field trial time in 2 months 2016, the period 180 days.
Field trial is divided into 6 field trial cells, and every cell plants 180 plants of pepper seedling, and seeding row spacing 0.4m ×
0.3m, area group distance 0.6m, plot area 30m2(6m×5m)。
The fertilising dosage of each cell is natural environmental-protective farmyard manure 30kg667m-2.Each cell applies embodiment 1 respectively
~6 natural environmental-protective farmyard manure.The field management measure of each cell is identical.
Effect example 1
Content of nitrite and content of beary metal are the important indicators for reflecting vegetables health quality.
The hygienic quality for the capsicum that Examples 1 to 6 natural environmental-protective farmyard manure field trial obtains is detected, detection refers to
Mark mainly has content of beary metal and content of nitrite.
Specific test result is shown in Table 1.
1 Quality of Capsicum testing result mg/kg of table
| Lead | Cadmium | Arsenic | Mercury | Nitrite | |
| Embodiment 1 | 0.027 | 0.0024 | 0.0099 | 0.0022 | 0.62 |
| Embodiment 2 | 0.024 | 0.0013 | 0.0096 | 0.0016 | 0.57 |
| Embodiment 3 | 0.022 | 0.00095 | 0.0074 | 0.0013 | 0.52 |
| Embodiment 4 | 0.020 | 0.00072 | 0.0061 | 0.0011 | 0.48 |
| Embodiment 5 | 0.019 | 0.00055 | 0.0059 | 0.1109 | 0.47 |
| Embodiment 6 | 0.0015 | 0.00049 | 0.0045 | 0.0002 | 0.41 |
According to《Pb in food tolerance limit》GB 14935-94, Pb≤0.2mg/kg；《Cadmium in foods limitation health
Standard》GB 15201-94, Cd≤0.05mg/kg；《Arsenic In Food tolerance limit》GB 4810-94, As≤0.5mg/kg；
《Mercury In Food tolerance limit》GB2762-94, Hg≤0.01mg/kg.It is found according to tables of data 1, capsicum in 6 embodiments
Content of beary metal all state health standards limitation within, meet the requirement of food hygienic standard.
Nitrite is a kind of strong carcinogen in human body, and the nitrite of human body intake is mainly from vegetables, therefore
Vegetables must be strictly controlled content of nitrite, and country is 699.7mg/ to nitrite concentration regulation highest limitation in vegetables
kg.It is found according to table 1, the content of nitrite in 6 embodiments is all within the scope of state health standards.
Effect example 2
The yield for the capsicum that Examples 1 to 6 natural environmental-protective farmyard manure field trial obtains is measured.
It is picked three times within chili growth week, plucking time is respectively July 10, July 25, and August 10th.
Since noon moisture loss is more, carpopodium should not fall off, and be easy to drag and hurt fruit branch.So picking capsicum at night into
Row.It can not also roll when picking, in order to avoid shake plant.
Specific test result is shown in Table 2.
2 yield of hot pepper test result table kg of table
Effect example 3
The nutrient composition content for the capsicum that Examples 1 to 6 natural environmental-protective farmyard manure field trial obtains is measured.
Vitamin C content uses 2,6- dichloroindophenol titration, carries out with reference to GB/T6195-1986.
Capsaicin content uses one acetone extract of kerosene, ultraviolet spectrophotometry, reference《The measurement of capsaicin content》(Shan
Western agricultural sciences, the 05th phase of nineteen ninety-five, Wang Jixiang, Liu Huirong).
Specific test result is shown in Table 3.
3 capsicum nutritional ingredient result table mg/100g of table
| Capsaicin content | Vc content | |
| Embodiment 1 | 76.34 | 124.59 |
| Embodiment 2 | 78.49 | 129.81 |
| Embodiment 3 | 80.56 | 132.28 |
| Embodiment 4 | 82.51 | 135.80 |
| Embodiment 5 | 82.92 | 136.96 |
| Embodiment 6 | 83.85 | 142.23 |
Effect example 4
The growth performance for the capsicum that Examples 1 to 6 natural environmental-protective farmyard manure field trial obtains is measured.
Test index includes root long, plant height, strain fresh weight and plant dry weight.
Specific test result is shown in Table 4.
4 growth performance result table of table
It is seen from the above data that there is significant difference (P < for the root long of Hot Pepper Seedling of the present invention, plant height, plant dry weight
0.05).This illustrates natural environmental-protective farmyard manure of the present invention, can significantly improve the growth of Hot Pepper Seedling, plays apparent strong
Seedling effect.Speculating its reason on the one hand is supported since biological organic liquid fertilizer provides a great number of elements and microelement etc. for Hot Pepper Seedling
Point, the auxin isoreactivity substance contained in another aspect biological organic liquid fertilizer enhances the resistance of Hot Pepper Seedling, from
And promote the growth of Hot Pepper Seedling.
Corncob is because its surface shape is loose, interior tissue is uniform, hole is flourishing and unique mandruka body structure
It can make the readily permeable inside into corncob of solution, to reach faster adsorption rate.And the strain in liquid bacterial fertilizer
Decomposition utilization preferably can be carried out to this substance.The embodiment of the present invention 2 is by obtaining one for corncob and liquid bacterial fertilizer combination
Kind good bio-organic fertilizer, a great number of elements and the microelements and plant growth stimulating such as nitrogen rich in, phosphorus, potassium
Element and humic acid etc., it is degradable, nontoxic, pollution-free compared with chemical fertilizer, it, can be to avoid chronic administration to person poultry harmless
It learns fertilizer and causes soil hardening, soil fertility can be increased, improve soil texture, keep soil moisture, plant height, yield to capsicum
There is significant impact with composite factors such as qualities.
Ferrihydrite possesses huge specific surface area and surface-active, and adsorption capacity is strong, the hydroxy functional group on surface can with it is peppery
The hydrophobic organic matter on green pepper seedling rhizome surface forms active force and has very strong adherence, can increase the adherence of solution,
Extend the residence time and action time of nutrient.Iron ore can be good at adsorbing nutriment in bacterial manure, with ferrihydrite and bacterial manure
In conjunction with granulated fertilizer obtained, the dry weight and fresh weight of plant seedlings of Hot Pepper Seedling is not only significantly improved, but also is also had to chlorophyll content a degree of
It improves, it is obvious to the growth-promoting effect of Pepper crops.For embodiment 4 by carrying out modified with reduction to ferrihydrite, making it possible to can
Nutriment in bacterial manure is preferably adsorbed, the role for serving as carrier carries out the transport of nutriment, promotes Pepper crops growth.
It should be appreciated that although this specification is described in terms of embodiments, but not each embodiment only includes one
A independent technical solution, for the sake of this narrating mode of specification is used for the purpose of clearly, those skilled in the art should be incited somebody to action
As a whole, the technical solutions in the various embodiments may also be suitably combined for specification, and forming those skilled in the art can
With the other embodiments of understanding.
Claims (10)
1. a kind of natural environmental-protective farmyard manure, which is characterized in that including fertilizer kernel, inner investment layer and outer coating layer.
2. the preparation method of natural environmental-protective farmyard manure according to claim 1, which is characterized in that including following three step：
Step 1：2~3g of konjak glucomannan and 0.2~1g of ammonium polyphosphate are uniformly mixed, mixture is obtained；It will mixing
Object is added in 30~80g of granulated fertilizer, stirs 10~30 minutes, so that mixture is evenly coated at granulated fertilizer surface；It is dry,
Obtain fertilizer kernel；
Step 2：1~2g of konjak glucomannan, 0.3~0.6g of sodium carbonate and 100~180 μ L of polyethylene glycol-400 are dissolved in
In 100~200mL water, stirs 30~50 minutes, obtain the konjak glucomannan solution of deacetylation；By the evil spirit of deacetylation
The fertilizer kernel that taro glucomannans solution and step 1 obtain is uniformly mixed, and is stirred 10~20 minutes, so that konjac mannan is poly-
Sugar juice is evenly coated at the surface of fertilizer kernel；It is dry, inner investment layer is formed in fertilizer core surface, is obtained containing inner investment
The fertilizer of layer；
Step 3：The fertilizer containing inner investment layer that 0.3~1g of water-retaining agent and step 2 are obtained is uniformly mixed, and stirs 5~10 points
Clock, so that water-retaining agent is evenly coated at the surface of inner investment layer；It is dry, outer coating layer is formed on the surface of interior coating layer, is obtained
The natural environmental-protective farmyard manure.
3. the preparation method of natural environmental-protective farmyard manure according to claim 2, which is characterized in that the granulated fertilizer is
Grain urea.
4. the preparation method of natural environmental-protective farmyard manure according to claim 2, which is characterized in that the granulated fertilizer passes through
Following methods obtain：Particulate vector is sterilized；By after sterilizing particulate vector and liquid bacterial fertilizer with 1:(0.8~1.5) (g/mL)
It is uniformly mixed, is subsequently placed in 28~30 DEG C and places 7~10 days, obtain the granulated fertilizer；Preferably, the particulate vector is made a living
Object charcoal and/or corn cob granule.
5. the preparation method of natural environmental-protective farmyard manure according to claim 2, which is characterized in that the granulated fertilizer passes through
Following methods obtain：Particulate vector is sterilized；By after sterilizing particulate vector, ferrihydrite or modified ferrihydrite and liquid bacterial fertilizer with
1：(0.01%~0.03%)：(0.8~1.5) (g/g/mL) is uniformly mixed, and is subsequently placed in 28~30 DEG C and is placed 7~10 days, obtains
To the granulated fertilizer；Preferably, the particulate vector is charcoal and/or corn cob granule.
6. the preparation method of natural environmental-protective farmyard manure according to claim 4 or 5, which is characterized in that the liquid bacterial fertilizer
Preparation process be：New fresh seaweed is taken, according to solid-liquid ratio 1：(0.2~0.3) (g/mL) plus water is ground 5~10 minutes, obtains sea
Algae homogenate；Complex enzyme is added according to the 0.1%~0.3% of seaweed homogenate quality in the pH to 6~7 for adjusting seaweed homogenate, 40~
50 DEG C enzyme digestion reaction 48~72 hours, the homogenate of seaweed after obtaining enzyme digestion reaction；Seaweed homogenate after taking enzyme digestion reaction, adds enzyme
Solution reaction after seaweed homogenate quality 0.15%~0.2% function microbial inoculum, adjust pH to 6~7, in 35~40 DEG C ferment 24~
48 hours；It is centrifuged 10~20 minutes with 3000~4000 revs/min later, collects supernatant, obtain the liquid bacterial fertilizer.
7. the preparation method of natural environmental-protective farmyard manure according to claim 5, which is characterized in that the preparation of the ferrihydrite
Process is：Prepare the FeCl of 0.3~0.5mol/L3·6H2The NaOH aqueous solution of O solution and 0.5~1.5mol/L；In stirring bar
Under part, NaOH aqueous solution is added to FeCl3·6H2In O solution, changed with pH in pH meter monitoring solution, until pH reach 6~
7, form suspension；Then suspension is stood 2~6 hours makes its aging, the suspension after obtaining aging；It will be outstanding after aging
Turbid is centrifuged 20~30 minutes in 2~4 DEG C, is collected sediment, is obtained the ferrihydrite.
8. the preparation method of natural environmental-protective farmyard manure according to claim 5, which is characterized in that the modified ferrihydrite
Preparation process is：The raw material of iron reduction culture medium is uniformly mixed according to formula, configuration obtains the iron reduction culture medium, wherein
The formula of iron reduction culture medium is：Na2HPO4·7H2O 12~13g/L, KH2PO4·7H2O 2~4g/L, NH4Cl 1~
2g/L, 2~3g/L of yeast extract, surplus are water；Then 3~4g of lactic acid is being added in every liter of iron reduction culture medium, as iron
The electron donor of oxide sterilizes 20~30 minutes in 120~121 DEG C, and the iron after being sterilized restores culture medium；Then to going out
Ferrihydrite is added in iron reduction culture medium after bacterium, makes 60~80mg/L of concentration of ferro element in culture medium；Be eventually adding according to
Volume ratio 2~3% be inoculated with iron-reducing bacterium, in 28~30 DEG C Anaerobic culturel 10~15 days, collect Anaerobic culturel product；Anaerobism is trained
It supports product to be centrifuged 20~30 minutes, collects bottom precipitation；Bottom precipitation is washed with deionized, it is dry, obtain the modification
Ferrihydrite.
9. the preparation method of natural environmental-protective farmyard manure according to claim 2, which is characterized in that the water-retaining agent is poly- third
The mixture of one or more of acrylamide, polysuccinimide, poly-aspartate.
10. application of the natural environmental-protective farmyard manure described in claim 1 in pepper planting.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117736029A (en) * | 2023-11-30 | 2024-03-22 | 中山市津海生物技术有限公司 | A method for producing seaweed extract |
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