CN108888995A - A kind of method of bamboo vinegar orientation extracting - Google Patents
A kind of method of bamboo vinegar orientation extracting Download PDFInfo
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- CN108888995A CN108888995A CN201810660432.3A CN201810660432A CN108888995A CN 108888995 A CN108888995 A CN 108888995A CN 201810660432 A CN201810660432 A CN 201810660432A CN 108888995 A CN108888995 A CN 108888995A
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- Prior art keywords
- bamboo vinegar
- extracting
- ether
- orientation
- methylene chloride
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- 235000017166 Bambusa arundinacea Nutrition 0.000 title claims abstract description 56
- 235000017491 Bambusa tulda Nutrition 0.000 title claims abstract description 56
- 241001330002 Bambuseae Species 0.000 title claims abstract description 56
- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 56
- 239000011425 bamboo Substances 0.000 title claims abstract description 56
- 239000000052 vinegar Substances 0.000 title claims abstract description 54
- 235000021419 vinegar Nutrition 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 28
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 60
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 claims abstract description 57
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000003068 static effect Effects 0.000 claims abstract description 4
- 239000000284 extract Substances 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 4
- 239000002027 dichloromethane extract Substances 0.000 claims description 3
- 239000012259 ether extract Substances 0.000 claims description 3
- XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 claims description 3
- 125000001033 ether group Chemical group 0.000 claims description 2
- 230000006837 decompression Effects 0.000 claims 1
- 239000012467 final product Substances 0.000 claims 1
- 230000000052 comparative effect Effects 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 230000000855 fungicidal effect Effects 0.000 description 6
- 239000000417 fungicide Substances 0.000 description 6
- 241000227653 Lycopersicon Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 4
- 150000001298 alcohols Chemical class 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 150000002989 phenols Chemical class 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 150000001335 aliphatic alkanes Chemical class 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 150000002576 ketones Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 150000007524 organic acids Chemical class 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003810 ethyl acetate extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
- B01D11/0492—Applications, solvents used
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/04—Freezing; Subsequent thawing; Cooling
- A23B7/0425—Freezing; Subsequent thawing; Cooling the material not being transported through or in the apparatus, with or without shaping, e.g. in the form of powder, granules or flakes
- A23B7/0433—Freezing; Subsequent thawing; Cooling the material not being transported through or in the apparatus, with or without shaping, e.g. in the form of powder, granules or flakes with packages or with shaping in the form of blocks or portions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/144—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor
- A23B7/152—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere comprising other gases in addition to CO2, N2, O2 or H2O ; Elimination of such other gases
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
- B01D11/0419—Solvent extraction of solutions which are liquid in combination with an electric or magnetic field or with vibrations
- B01D11/0423—Applying ultrasound
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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- General Health & Medical Sciences (AREA)
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- General Chemical & Material Sciences (AREA)
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- Food Science & Technology (AREA)
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- Forests & Forestry (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
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- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
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- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of methods of bamboo vinegar orientation extracting, by the way that ether, methylene chloride and ethyl acetate is added in bamboo-vinegar respectively, it is stood after extracting 5-10min under ultrasonic wave and electromagnetic wave auxiliary, static 1h in thermostat water bath under the conditions of being placed in 20~30 DEG C, after liquid layered, subnatant is taken, the ethyl acetate, ether and methylene chloride of the not year-on-year part of use cooperate refined bamboo vinegar, it can be modulated according to application industry, keep its specific aim stronger.
Description
Technical field
The present invention relates to the production field of bamboo extractive, more particularly to a kind of side of bamboo vinegar orientation extracting
Method.
Background technique
Bamboo vinegar is a large amount of byproducts that can be obtained in bamboo pyrolysis, is water-soluble liquid mixture, purposes
It is very extensive, have a special efficacy in fields such as agricultural, medical treatment, health cares, while can in soil improvement, remove smell and food
It saves aspect to play a significant role, deeply probing into bamboo vinegar is unfolded in more and more experts and scholars both at home and abroad.
According to Japan and domestic expert's research, bamboo vinegar is a kind of mixture that constituent is considerably complicated, main component
It is therefore to be surveyed using GC-MS to bamboo vinegar component water (accounting for about 80%), followed by organic acid, phenols, ketone, alcohols etc.
It must be by being dehydrated refinement treatment before examination analysis.Domestic scholars select calcium nitrate, anhydrous sodium sulfate be desiccant to bamboo vinegar into
Row goes water process；Also have and handled using the way of distillation and opposed polarity extraction.
But there are larger differences by the bamboo vinegar Comparative of different disposal method, at present in existing bamboo vinegar extracting method
Extracted by distillation, containing a large amount of invalid components, and existing extracting process its cannot extract a variety of types comprehensively
Effective component.
Summary of the invention
In view of the deficiencies of the prior art, the present invention intends to provide a kind of method of bamboo vinegar orientation extracting,
The effective components such as organic acid therein, phenols, ketone, alcohols can efficiently be extracted.
To achieve the above object, the present invention provides following technical solutions：A kind of method of bamboo vinegar orientation extracting, 1) it presses
Mass fraction takes 80-90 parts of raw liquid of bamboo vinegar, is concentrated, and obtains the bamboo vinegar concentrated liquor that quality halves；2) it is added 5-10 times
Mixed organic solvents extractant, mixed organic solvents extractant is ether, methylene chloride and ethyl acetate, in ultrasonic wave and
It is stood after the lower extraction 5-10min of electromagnetic wave auxiliary；
3) pretreatment sample of step 2) acquisition, static 1h in the thermostat water bath under the conditions of being placed in 20~30 DEG C, liquid are taken
After body layering, subnatant is taken.
Specifically, extraction process passes sequentially through ethyl acetate, ether and methylene chloride and is extracted in step 2, passes through heat
Refluxing extraction, obtains acetic acid ethyl ester extract, ether extract and dichloromethane extract, combined extract, and concentrate drying is
?.
Specifically, the mass parts ratio of organic solvent extractant ether therein, methylene chloride and ethyl acetate be 1~
20：1~20：10~50.
Specifically, the extracting bamboo vinegar that step 3 obtains is kept in dark place.
Specifically, the ultrasonic wave in step 2 is 30-45HZ, electromagnetic wave 120-150W.
Specifically, the number extracted in step 2 is 1-3 times；Preferably 3 times.
Specifically, the concentration is to be concentrated under reduced pressure.
Beneficial effects of the present invention are：The present invention extracts bamboo vinegar, by mixed organic extractant according to bamboo
Application of the vinegar liquid in different field carries out bamboo vinegar using ethyl acetate, ether and the methylene chloride of different quality part ratio
Extraction can obtain the bamboo vinegar extract of different content organic acid, phenols, ketone, alcohols composition,
For acids and phenols, ethyl acetate can extract to obtain a greater variety of ingredients, and methylene chloride can then obtain more
The ingredient of more contents；Ether is opposite to be more suitable for extracting alcohols material, while can obtain the more letones of relative amount, therewith
It compares, ethyl acetate can then obtain a greater variety of letones；Extracting for aldehyde material, then select ethyl acetate more to close
It is suitable；Aromatic compounds and other class distribution contents are not high, select ethyl acetate extraction effect best；
It is limited by the physicochemical property and molecular structure difference of single solvent, can not be taken when extracting bamboo vinegar using single solvent
Optimum efficiency is obtained, the ingredient Species differences measured are big, therefore using ethyl acetate, ether and the methylene chloride cooperation of not year-on-year part
Refined bamboo vinegar can be modulated according to application industry, keep its specific aim stronger.
Specific embodiment
As long as it is pure above that the purity of the raw materials used in the present invention reaches chemistry, source is commercially available.
Embodiment 1：A kind of bamboo vinegar is used to prepare the method for extracting of fungicide
1) mass fraction is pressed, 80-90 part raw liquid of bamboo vinegar is taken, is concentrated, adjusting its pH is 6.5, at 60 DEG C, vacuum degree
It distills under conditions of 0.09MPa, stops after distillating 50% stoste volume, be stripped of tar, it is preferably that the bamboo vinegar after distillation is dense
Contracting liquid is filtered decoloration by bamboo charcoal particle；
2) 10 times of mixed organic solvents extractant is added, passes sequentially through ethyl acetate, ether and methylene chloride, is returned in heat
It under the conditions of stream, and is extracted under 30HZ ultrasonic wave and 120W electromagnetic wave auxiliary, is mentioned by the auxiliary of ultrasonic wave and electromagnetic wave
It takes, greatly accelerates its extraction rate, the mass parts ratio of ether, methylene chloride and ethyl acetate is 1：1：10, extract 5-
It is stood after 10min, obtains acetic acid ethyl ester extract, ether extract and dichloromethane extract, repeated the above steps 3 times, closed
And extracting solution, it is concentrated and dried up to pretreatment sample；
3) pretreatment sample of step 2) acquisition, static 1h in the thermostat water bath under the conditions of being placed in 20~30 DEG C, liquid are taken
After body layering, subnatant is taken, obtained extracting bamboo vinegar is kept in dark place.
A kind of bamboo vinegar of embodiment 2 is used to prepare the method for extracting of fungicide, ether, methylene chloride and second in step 2)
The mass parts ratio of acetoacetic ester is 3：1：10.
A kind of bamboo vinegar of embodiment 3 is used to prepare the method for extracting of fungicide, ether, methylene chloride and second in step 2)
The mass parts ratio of acetoacetic ester is 1：1：20.
A kind of bamboo vinegar of comparative example 1 is used to prepare the method for extracting of fungicide, carries out in step 2) only with ether
It extracts.
A kind of bamboo vinegar of comparative example 2 is used to prepare the method for extracting of fungicide, only with methylene chloride in step 2)
It extracts.
A kind of bamboo vinegar of comparative example 3 is used to prepare the method for extracting of fungicide, only with ethyl acetate in step 2)
It extracts.
Using tomato as object, start within 5 days after bearing fruit to carry out spraying processing, be sprayed every 10 days once, it is specific as follows：Control
Group 1 is trichoderma and streptococcus lactis, and using clear water as blank control, control group 1 is sprayed after diluting 1000 times, is implemented
Effective component and 1 mass parts of control group etc. in the bamboo vinegar extracting solution of example 1-3 and comparative example 1-3 is about identical, by embodiment 1-3
100 times are diluted with the experimental group of comparative example 1-3, fruit 8 is picked when mature, is sorted, is selected consistent fruit uniform in size, is rejected
Machinery wound, inferior and pest and disease damage fruit, disperse field heat, are sub-packed in turnover box by every bag of 10kg, after the processing for 24 hours of room temperature callus
It is transferred to 15.0 ± 1.0 DEG C, 90% freezer of RH, sterilization processing, storage 5 are carried out using the ozone of ozone generator production 10mg/m3
After it, temperature of ice house is adjusted to 9.0 ± 1.0 DEG C and is stored.Count storage period different disposal group tomato rotting mechanism and
Go rotten index, the results are shown in Table 1-2.
1 Different treatments of table extracting bamboo vinegar sprays its rotting mechanism during storage after tomato
2 Different treatments of table extracting bamboo vinegar sprays its mildew index during storage after tomato
Embodiment 4：A kind of bamboo vinegar is used to prepare the method for extracting of foreign smell eliminating agent, ether, dichloromethane in step 2)
The mass parts of alkane and ethyl acetate ratio is 3：20：10.
Embodiment 5：A kind of bamboo vinegar is used to prepare the method for extracting of foreign smell eliminating agent, ether, dichloromethane in step 2)
The mass parts of alkane and ethyl acetate ratio is 1：20：10.
Embodiment 6：A kind of bamboo vinegar is used to prepare the method for extracting of foreign smell eliminating agent, ether, dichloromethane in step 2)
The mass parts of alkane and ethyl acetate ratio is 1：10：10.
Bamboo vinegar extracting solution prepared by embodiment 4-6 is added in pig manure dirt with the additive amount of 10ml/L, after 48 hours
The air of the 1.5 meters of positions in detecting distance ground, deodorizing effect such as table 3；
The deodorizing effect of 3 Different treatments of table extracting bamboo vinegar
Above-mentioned pig manure dirt is made of solid, liquid state excrement and flushing water, wherein the weight of solid-state excrement, liquid excrement, flushing water
Amount is than being 1:1.2:5.6；The detection of ammonia uses Berthelot spectrophotometry, and the detection of hydrogen sulfide uses silver nitrate colorimetric
Method.
The main component and content of the obtained bamboo vinegar extracting solution of comparative example 1-3 are different, result such as table 4-5；
4 Different treatments of table extract bamboo vinegar main component
Explanation："/" expression is not detected.
5 Different treatments of table extract bamboo vinegar each component type and content
Explanation："/" expression is not detected.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (7)
1. a kind of method of bamboo vinegar orientation extracting, it is characterised in that：
1) mass fraction is pressed, 80-90 parts of raw liquid of bamboo vinegar is taken, is concentrated, obtain the bamboo vinegar concentrated liquor that quality halves；
2) 5-10 times of mixed organic solvents extractant is added, mixed organic solvents extractant is ether, methylene chloride and second
Acetoacetic ester is stood after extracting 5-10min under ultrasonic wave and electromagnetic wave auxiliary；
3) pretreatment sample of step 2) acquisition, static 1h in the thermostat water bath under the conditions of being placed in 20~30 DEG C, liquid point are taken
After layer, subnatant is taken.
2. a kind of method of bamboo vinegar orientation extracting according to claim 1, it is characterised in that：Extraction process in step 2
It passes sequentially through ethyl acetate, ether and methylene chloride to be extracted, by circumfluence distillation, obtains acetic acid ethyl ester extract, second
Ether extract and dichloromethane extract, combined extract are concentrated and dried to obtain the final product.
3. a kind of method of bamboo vinegar orientation extracting according to claim 2, it is characterised in that：The organic solvent extracts
The mass parts ratio of agent ether therein, methylene chloride and ethyl acetate is 1~20：1~20：10~50.
4. a kind of method of bamboo vinegar orientation extracting according to claim 1, it is characterised in that：The extracting that step 3 obtains
Bamboo vinegar is kept in dark place.
5. a kind of method of bamboo vinegar orientation extracting according to claim 4, it is characterised in that：Ultrasonic wave in step 2
For 30-45HZ, electromagnetic wave 120-150W.
6. a kind of method of bamboo vinegar orientation extracting according to claim 5, it is characterised in that：It is extracted in step 2
Number be 1-3 times；Preferably 3 times.
7. a kind of method of bamboo vinegar orientation extracting according to claim 5, it is characterised in that：The concentration is decompression
Concentration.
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| CN111264302A (en) * | 2020-03-09 | 2020-06-12 | 中国林业科学研究院林产化学工业研究所 | Edible fungus growth promoter and preparation method and application thereof |
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| CN107880922A (en) * | 2017-11-24 | 2018-04-06 | 华益药业科技(安徽)有限公司 | One kind is without phenol bamboo vinegar and preparation method thereof |
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| CN111264302A (en) * | 2020-03-09 | 2020-06-12 | 中国林业科学研究院林产化学工业研究所 | Edible fungus growth promoter and preparation method and application thereof |
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