CN108456545A - A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing - Google Patents
A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing Download PDFInfo
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- CN108456545A CN108456545A CN201810069471.6A CN201810069471A CN108456545A CN 108456545 A CN108456545 A CN 108456545A CN 201810069471 A CN201810069471 A CN 201810069471A CN 108456545 A CN108456545 A CN 108456545A
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- 239000000052 vinegar Substances 0.000 title claims abstract description 119
- 235000021419 vinegar Nutrition 0.000 title claims abstract description 119
- 239000002023 wood Substances 0.000 title claims abstract description 116
- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 91
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 235000012055 fruits and vegetables Nutrition 0.000 title claims abstract description 14
- 240000005561 Musa balbisiana Species 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 58
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 53
- 238000000034 method Methods 0.000 claims abstract description 52
- 108090000790 Enzymes Proteins 0.000 claims abstract description 46
- 102000004190 Enzymes Human genes 0.000 claims abstract description 46
- 244000235858 Acetobacter xylinum Species 0.000 claims abstract description 34
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims abstract description 34
- 235000019441 ethanol Nutrition 0.000 claims abstract description 27
- 241000234295 Musa Species 0.000 claims abstract description 24
- 239000001913 cellulose Substances 0.000 claims abstract description 24
- 229920002678 cellulose Polymers 0.000 claims abstract description 24
- 230000008569 process Effects 0.000 claims abstract description 23
- 229920005610 lignin Polymers 0.000 claims abstract description 20
- 239000006041 probiotic Substances 0.000 claims abstract description 19
- 235000018291 probiotics Nutrition 0.000 claims abstract description 19
- 230000001580 bacterial effect Effects 0.000 claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims description 69
- 229940088598 enzyme Drugs 0.000 claims description 45
- 238000011534 incubation Methods 0.000 claims description 45
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 44
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 40
- 238000004821 distillation Methods 0.000 claims description 38
- 239000007788 liquid Substances 0.000 claims description 36
- 239000012141 concentrate Substances 0.000 claims description 29
- 238000000605 extraction Methods 0.000 claims description 25
- 241000235342 Saccharomycetes Species 0.000 claims description 22
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 238000010438 heat treatment Methods 0.000 claims description 20
- 239000002609 medium Substances 0.000 claims description 20
- 239000001814 pectin Substances 0.000 claims description 20
- 229920001277 pectin Polymers 0.000 claims description 20
- 235000010987 pectin Nutrition 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 241000589220 Acetobacter Species 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 18
- 238000000199 molecular distillation Methods 0.000 claims description 17
- 239000010902 straw Substances 0.000 claims description 16
- 229920001817 Agar Polymers 0.000 claims description 15
- 239000001888 Peptone Substances 0.000 claims description 15
- 108010080698 Peptones Proteins 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 230000004913 activation Effects 0.000 claims description 15
- 239000008272 agar Substances 0.000 claims description 15
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 15
- 235000019319 peptone Nutrition 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 229940041514 candida albicans extract Drugs 0.000 claims description 14
- 239000012138 yeast extract Substances 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 13
- 108010059892 Cellulase Proteins 0.000 claims description 12
- 229940106157 cellulase Drugs 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 230000000529 probiotic effect Effects 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 229920001661 Chitosan Polymers 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 4
- 230000008020 evaporation Effects 0.000 claims description 4
- 230000020477 pH reduction Effects 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 2
- 238000010025 steaming Methods 0.000 claims 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 abstract description 17
- 239000003795 chemical substances by application Substances 0.000 abstract description 17
- 150000002148 esters Chemical class 0.000 abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 5
- 238000012545 processing Methods 0.000 abstract description 5
- 229920002488 Hemicellulose Polymers 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 240000008790 Musa x paradisiaca Species 0.000 description 67
- 238000012360 testing method Methods 0.000 description 29
- 239000000835 fiber Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000007602 hot air drying Methods 0.000 description 4
- 238000013517 stratification Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 235000000318 Bindesalat Nutrition 0.000 description 3
- 244000106835 Bindesalat Species 0.000 description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 3
- 244000141359 Malus pumila Species 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical group 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 238000000197 pyrolysis Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- -1 hydrazine compound Chemical class 0.000 description 1
- 239000003752 hydrotrope Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- QUCDWLYKDRVKMI-UHFFFAOYSA-M sodium;3,4-dimethylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1C QUCDWLYKDRVKMI-UHFFFAOYSA-M 0.000 description 1
- 239000003516 soil conditioner Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10C—WORKING-UP PITCH, ASPHALT, BITUMEN, TAR; PYROLIGNEOUS ACID
- C10C5/00—Production of pyroligneous acid distillation of wood, dry distillation of organic waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Materials Engineering (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to wood vinegar processing technique fields, more particularly to a kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing, banana stalk contains abundant cellulose, hemicellulose and lignin, it is a kind of optimum feed stock of processing wood vinegar, the application pre-processes banana stalk using biological enzyme, and probiotics reprocesses the banana stalk after enzymolysis the wood vinegar yield that can effectively improve banana stalk；By ethyl alcohol domestication, acetobacter xylinum, by domestication of acid-resistance, its decomposition and inversion ability is stronger by acetone domestication, yeast for the acetobacter xylinum of the application, more acetums can be generated, the wood vinegar of higher purity, high yield will be produced through distilling, purifying again by being processed into after acetum, wood vinegar will improve its sour and ester content after refined, concentration, reduce hydrazine content, it can inhibit bacterial growth, play the role of preserving fruit and vegetable utilizing, be a kind of safely and effectively antistaling agent.
Description
【Technical field】
The present invention relates to wood vinegar processing technique field, more particularly to a kind of banana wood vinegar systems applied to preserving fruit and vegetable utilizing
Preparation Method.
【Background technology】
Wood vinegar derives from plant, is a kind of liquid mixture with dense smoke.Stalk, branch, trunk, fruit
Shell, corncob and sawdust will produce gas during high temperature pyrolysis carbonizes, by these gas condensing recoveries, so that it may with
To crude wood vinegar.Contain gas chromatography in wood vinegar, main component is acids, phenols, ketone and aldehydes, is in addition also contained
There are some alcohols, esters and Ammonia etc., wherein there are about more than 500 kinds for the substance being detected.It is micro- there are many also containing in wood vinegar
Secondary element, such as potassium, calcium, magnesium, zinc, manganese, iron.Its ingredient of the wood vinegar obtained from different plants is different.Together
A kind of plant the ingredient for the wood vinegar being collected into and is measured also different with the variation of pyrolysis temperature.At this stage, wood vinegar
By as uses such as soil conditioner, Plant leaf fertilizer, prevention and control of plant diseases, pest control drug, food preservatives.Studies have shown that it also can quilt
It applies in medicinal industry and animal husbandry.
There is good fresh-keeping function containing a large amount of organic compound in wood vinegar.Banana stalk is relatively soft, in stalk
Content of cellulose is 80%-90%, hemicellulose level 1%-5%, content of lignin 1%-3%, pectin and the hydrotrope
Content can reach 3%-9%, it can be seen that, it is to prepare that abundant cellulose, hemicellulose and lignin are contained in banana stalk
The optimum feed stock of wood vinegar.But a certain amount of hydrazine compound is will produce due to dealing with improperly in conventional wood vinegar, since hydrazine is toxic,
The safety of antistaling agent can be influenced in food fresh keeping by applying.Therefore, how effectively banana stalk is processed, improves wood
The yield of vinegar liquid, the hydrazine content for reducing wood vinegar, it is this field skill that safe and nontoxic wood vinegar, which is applied to preserving fruit and vegetable utilizing field,
Art personnel's technical issues that need to address.
【Invention content】
In view of the above, it is necessary to a kind of banana wood vinegar is produced using banana stalk, it can be effectively to banana stalk
It is processed, improves the yield of wood vinegar, reduce the hydrazine content of wood vinegar, safe and nontoxic wood vinegar is protected applied to fruits and vegetables
Fresh field, further increase banana waste material utilizes field, utility value, the added value of product of raising banana waste material.
In order to achieve the above objectives, the technical solution adopted in the present invention is：
A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing, described method includes following steps：
(1) pretreatment of raw material：With biological enzyme solutions it is 1 according to mass ratio by banana stalk:2h-3h, mistake are impregnated in 2-5 mixing
By filter residue and probiotic solution according to mass ratio it is 1 after filter:2-5 mixing impregnate 1h-2h, taken after filtering filter residue temperature be 50
Air-dried, crushed in DEG C -60 DEG C of air drier, and screened by the sieve of -150 mesh of 100 mesh, then with mass concentration
According to solid-liquid mass ratio it is 1 for the Klorvess Liquid of 2%-5%:4-6 is mixed, and is soaked under conditions of temperature is -5 DEG C~-2 DEG C
10h-12h is steeped, is then placed in the air drier that temperature is 50 DEG C -60 DEG C and is air-dried to obtain the banana stalk pre-processed
Powder；
(2) wood vinegar is crude：According to mass ratio it is 50-55 by the banana stalk powder of step (1) and potassium powder:1 is mixed
It closes, places into distillation reactor, then nitrogen is passed through in distillation reactor, oxygen is discharged, nitrogen flow 200-
300mL/min；Distillation reactor is slowly heated to 30 DEG C -40 DEG C according to the heating rate of 5 DEG C/min-8 DEG C/min later, it
Heated at constant temperature afterwards collects the liquid of cold energy in distillation reactor, until banana stalk powder decomposes completely, obtains the crude wooden vinegar
Liquid；
(3) wood vinegar is refined：The crude wood vinegar that step (2) obtains is put into molecular distillation reaction kettle and is once divided
The distillate of single flash is carried out quadratic component and distills to obtain acetum pyrolignosum rectificatum liquid by son distillation；Molecular distillation and two
The distillation pressure of secondary molecular distillation is 100-200Pa, and charging rate is 3-4mL/min, and rotating speed is 100-200r/min；
The heating temperature of molecular distillation is 50-60 DEG C, and the heating temperature of quadratic component distillation is 70-80 DEG C；
(4) wood vinegar concentrates：According to volume ratio it is 2-3 by the acetum pyrolignosum rectificatum liquid of step (3) and ether:1 is mixed, quiet
Layering extraction is set, ether extraction phase is drawn, repeats extraction 6-8 times, merges ether extraction phase, heat and steam in 40-50 DEG C of water-bath
Hair removal ether obtains wood vinegar concentrate I；It is the NaHCO of 5%-8% by the acetum pyrolignosum rectificatum liquid of step (3) and mass concentration3
It is 2-3 according to volume ratio:1 is mixed, and the pH value of mixed liquor is adjusted to by the NaOH for being again then 3%-5% with mass concentration
5, stratification draws the water layer after extraction acidification, repeats extraction 3-4 times, combining water layer extract layer, then by extract layer and acetic acid
Ethyl ester solution is 1 according to volume ratio:1-3 is mixed, and is drawn ethyl acetate phase removal ethyl acetate and is obtained wood vinegar concentrate
II merges wood vinegar concentrate I and wood vinegar concentrate II and obtains the wood vinegar concentrate.
Further, the biological enzyme solutions of the step (1) comprise the following components in parts by weight：12 parts -17 parts of fibre
The plain enzyme of dimension, 21 parts -29 parts of pectase and 17 parts -26 parts of lignin-degrading enzymes.
Further, the enzyme activity intensity of the cellulase is 700U/g-900U/g；The enzyme activity intensity of pectase is
1000U/g-1200U/g；The enzyme activity intensity of lignin-degrading enzymes is 1200U/g-1500U/g.
Further, the probiotic solution of the step (1) comprises the following components in parts by weight：Every gram of viable count is
1.6×107CFU/g~8.7 × 107CFU/g acetobacter xylinums, every gram of viable count are 2.3 × 108CFU/g~5.8 × 108CFU/g
Saccharomycete and every gram of viable count are 1.8 × 109CFU/g~4.5 × 109CFU/g acetobacters.
Further, the acclimation method of the acetobacter xylinum is：Acetobacter xylinum is placed in domestication culture medium I and is cultivated, is supervised
Survey the content of acetone in culture medium；Terminate first phase culture when content of acetone reaches 6mg/L in culture medium, and by incubation time
It is denoted as T1；The first phase cultivate after carry out second phase culture, pick out the first phase culture after bacterial strain be placed in it is identical
It is cultivated in the culture of formula, and repeats first phase incubation, terminate second when the content of acetone in culture medium reaches 6mg/L
Phase cultivates, and incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/5T1When, complete domestication process；It is described
Activation medium I by as follows at being grouped as：Pectin that cellulose that mass concentration is 100mg/L, mass concentration are 50mg/L,
Yeast extract that chitosan that lignin that mass concentration is 50mg/L, mass concentration are 5mg/mL, mass concentration are 15mg/mL,
Agar that peptone that mass concentration is 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I is total
Volume is 1L, pH 6.5.
Further, the acclimation method of the saccharomycete is that saccharomycete is placed in domestication medium ii to cultivate, monitoring training
Support the ethanol content in base；Terminate first phase culture when ethanol content reaches 100mg/L in culture medium, and incubation time is remembered
For T1；The first phase cultivate after carry out second phase culture, pick out the first phase culture after bacterial strain be placed in identical match
It is cultivated in the culture of side, and repeats first phase incubation, terminate second when the content of ethyl alcohol in culture medium reaches 100mg/L
Phase cultivates, and incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/2T1When, complete domestication process；It is described
Activation medium II by as follows at being grouped as：Pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L,
Glucose that starch that lignin that mass concentration is 10mg/L, mass concentration are 15mg/L, mass concentration are 100mg/mL, matter
Measure the yeast extract of a concentration of 15mg/mL, the agar that the peptone that mass concentration is 10mg/mL, mass concentration are 10mg/mL, its
Remaining is banana straw juice, and culture medium I total volumes are 1L, pH 6.8.
Further, the acclimation method of the acetobacter is that acetobacter xylinum is placed in domestication culture medium I to cultivate, and is monitored
PH value in culture medium；Terminate first phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T1；The first phase
Second phase culture is carried out after culture, is picked out the bacterial strain after first phase culture and is placed in the culture of same recipe and trains
It supports, and repeats first phase incubation, constantly terminate second phase culture when pH in culture medium value reaches 3, and incubation time is remembered
For T2, so repeating the n phases cultivates, and works as Tn=1/3T1When, complete domestication process；The activation medium I by as follows at
It is grouped as：Pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are the wooden of 10mg/L
Element, mass concentration be 200mg/L ethyl alcohol, mass concentration be 100mg/mL glucose, mass concentration be 15mg/mL yeast
Agar that peptone that cream, mass concentration are 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I
Total volume is 1L, pH 7.
The present invention has the advantages that：
1, banana stalk of the invention contains abundant cellulose, hemicellulose and lignin, is a kind of processing wood vinegar
Optimum feed stock, meanwhile, a large amount of pectin is also contained in banana stalk, pectin is easy to be carbonized caking, it is not easy to decompose generation
How wood vinegar effectively removes the yield of pectin, raising banana wood vinegar in the technique for producing wood vinegar using banana stalk
It is the application technical issues that need to address；Applicant pre-processes banana stalk, uses biological enzyme to banana first
Stalk is pre-processed, and abundant cellulase, pectase and lignin-degrading enzymes are contained in biological enzyme, and pectase can effectively be gone
Except pectin, cellulose and lignin-degrading enzymes can effectively decompose cellulose and lignin, the Portugal for being easy to be decomposed is generated
The small-molecule substances such as grape sugar, still, in banana stalk, fiber content is higher, and cellulase and lignoenzyme only is used only also not
It can be decomposed completely, moreover, cellulase and lignoenzyme are weaker to the capacity of decomposition of cellulose, perfume (or spice) can not be decomposed completely
The cellulose and lignin of any of several broadleaf plants stalk improve the yield of wood vinegar in order to obtain the active nutrients of more small molecule, application
People, which studies, to find also effectively to decompose enzymolysis product using probiotics after digesting；Contain acetobacter xylinum, ferment in probiotics
Female bacterium and acetobacter, in composition, acetobacter xylinum first degrades to banana fiber, generates the products such as cellulose, glucose,
But since banana fiber will produce a certain amount of acetone after acetobacter xylinum is degraded, inhibit to make since acetone has bacterium
With, if the resistant to acetone ability that will effectively improve acetobacter xylinum can be tamed first to acetobacter xylinum, increase acetobacter xylinum pair
The capacity of decomposition of banana fiber, banana fiber generate the products such as a certain amount of glucose after decomposing, and saccharomycete can send out glucose
Ferment generates ethyl alcohol, and ethanol content is excessively high to have yeast certain inhibiting effect, in order to improve the resistance to ethyl alcohol ability of yeast, applicant
Yeast is tamed, ethanol production is further increased；Ethyl alcohol can generate acetic acid after acetobacter ferments, and acetic acid contains
Measure it is excessively high acetobacter can be inhibited to grow, therefore, acetobacter after strong acid is tamed, can filter out with more acid-fast ability,
The higher acetobacter of fermentation efficiency.Acetic acid content can be improved after biological enzyme and probiotics processing in banana stalk, follow-up to pass through
It crosses distillation, purify the wood vinegar that will produce higher purity, high yield, wood vinegar will improve it after refined, concentration
The content of acid and ester；In banana wood vinegar other than containing acid and ester content, also contain abundant aldehydes, ketone, phenols, cypress
The substances such as brain, these ingredients will effectively inhibit bacterial growth, play the role of preserving fruit and vegetable utilizing, meanwhile, wood vinegar is from plant
The active ingredient of middle extraction will not cause the residual of chemical composition, be a kind of safely and effectively antistaling agent.
【Specific implementation mode】
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing is present embodiments provided, this method includes following step
Suddenly：
(1) pretreatment of raw material：With biological enzyme solutions it is 1 according to mass ratio by banana stalk:2h is impregnated in 2 mixing, after filtering
According to mass ratio it is 1 by filter residue and probiotic solution:1h is impregnated in 2 mixing, and the hot air drying that filter residue is 50 DEG C in temperature is taken after filtering
Air-dried, crushed in dry machine, and by 100 mesh sieve screen, then with mass concentration be 2% Klorvess Liquid according to
Solid-liquid mass ratio is 1:4 mixing, and 10h is impregnated under conditions of temperature is -5 DEG C, it is then placed in the hot air drying that temperature is 50 DEG C
It is air-dried to obtain the banana stalk powder pre-processed in dry machine；
(2) wood vinegar is crude：According to mass ratio it is 50 by the banana stalk powder of step (1) and potassium powder:1 is mixed,
It places into distillation reactor, then nitrogen is passed through in distillation reactor, oxygen is discharged, nitrogen flow 200mL/min；
Distillation reactor is slowly heated to 30 DEG C according to the heating rate of 5 DEG C/min later, later heated at constant temperature, collects distillation reaction
The liquid of cold energy in device obtains crude wood vinegar until banana stalk powder decomposes completely；
(3) wood vinegar is refined：The crude wood vinegar that step (2) obtains is put into molecular distillation reaction kettle and is once divided
The distillate of single flash is carried out quadratic component and distills to obtain acetum pyrolignosum rectificatum liquid by son distillation；Molecular distillation and two
The distillation pressure of secondary molecular distillation is 100Pa, and charging rate is 3mL/min, and rotating speed is 100r/min；Described primary point
The heating temperature of son distillation is 50 DEG C, and the heating temperature of quadratic component distillation is 70 DEG C；
(4) wood vinegar concentrates：According to volume ratio it is 2 by the acetum pyrolignosum rectificatum liquid of step (3) and ether:1 is mixed, and is stood
Layering extracts, and draws ether extraction phase, repeats extraction 6 times, merges ether extraction phase, the heating evaporation removal second in 40 DEG C of water-baths
Ether obtains wood vinegar concentrate I；The NaHCO for being 5% by the acetum pyrolignosum rectificatum liquid of step (3) and mass concentration3It is according to volume ratio
2:1 is mixed, and the pH value of mixed liquor is adjusted to 5 by the NaOH for being again then 3% with mass concentration, and stratification draws extraction
The water layer after acidification is taken, repeats to extract 3 times, combining water layer extract layer, then by extract layer and ethyl acetate solution according to volume ratio
It is 1:1 is mixed, and is drawn ethyl acetate phase removal ethyl acetate and is obtained wood vinegar concentrate II, merges wood vinegar concentrate I
The wood vinegar concentrate is obtained with wood vinegar concentrate II.
Wherein, the biological enzyme solutions of the present embodiment comprise the following components in parts by weight：12 parts of cellulase, 21 parts
Pectase and 17 parts of lignin-degrading enzymes.Wherein, the enzyme activity intensity of cellulase is 700U/g；The enzyme activity intensity of pectase is
1000U/g；The enzyme activity intensity of lignin-degrading enzymes is 1200U/g.
Wherein, the probiotic solution of the present embodiment comprises the following components in parts by weight：Every gram of viable count is 1.6 ×
107CFU/g acetobacter xylinums, every gram of viable count are 2.3 × 108CFU/g saccharomycete and every gram of viable count are 1.8 × 109CFU/g vinegar
Acidfast bacilli.
In order to obtaining more efficient acetobacter xylinum, saccharomycete and acetobacter, need to acetobacter xylinum, saccharomycete and
Acetobacter is tamed.
The acclimation method of above-mentioned acetobacter xylinum is：Acetobacter xylinum is placed in domestication culture medium I and is cultivated, is monitored in culture medium
Content of acetone；Terminate first phase culture when content of acetone reaches 6mg/L in culture medium, and incubation time is denoted as T1；The
One phase carried out second phase culture after cultivating, and picks out the bacterial strain after first phase culture and is placed in the culture of same recipe
Middle culture, and first phase incubation is repeated, terminate second phase culture when the content of acetone in culture medium reaches 6mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/5T1When, complete domestication process；The activation medium
I by as follows at being grouped as：Pectin that cellulose that mass concentration is 100mg/L, mass concentration are 50mg/L, mass concentration are
Yeast extract that chitosan that the lignin of 50mg/L, mass concentration are 5mg/mL, mass concentration are 15mg/mL, mass concentration are
Agar that the peptone of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes are 1L, pH
It is 6.5.
The acclimation method of above-mentioned saccharomycete is that saccharomycete is placed in domestication medium ii to cultivate, and monitors the second in culture medium
Alcohol content；Terminate first phase culture when ethanol content reaches 100mg/L in culture medium, and incubation time is denoted as T1；First
Phase cultivate after carry out second phase culture, pick out the first phase culture after bacterial strain be placed in the culture of same recipe
Culture, and first phase incubation is repeated, terminate second phase culture when the content of ethyl alcohol in culture medium reaches 100mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/2T1When, complete domestication process；The activation medium
II by as follows at being grouped as：Pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are
Glucose that starch that the lignin of 10mg/L, mass concentration are 15mg/L, mass concentration are 100mg/mL, mass concentration are
Agar that peptone that the yeast extract of 15mg/mL, mass concentration are 10mg/mL, mass concentration are 10mg/mL, remaining be banana
Straw juice, culture medium I total volumes are 1L, pH 6.8.
The acclimation method of above-mentioned acetobacter is that acetobacter xylinum is placed in domestication culture medium I to cultivate, and is monitored in culture medium
PH value；Terminate first phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T1；First phase culture terminates
Second phase culture is carried out afterwards, is picked out the bacterial strain after first phase culture and is placed in the culture of same recipe and cultivates, lays equal stress on
Multiple first phase incubation constantly terminates second phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T2, such as
This repeats the n phases and cultivates, and works as Tn=1/3T1When, complete domestication process；The activation medium I by as follows at being grouped as：
Lignin that pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are 10mg/L, quality
Yeast extract that glucose that the ethyl alcohol of a concentration of 200mg/L, mass concentration are 100mg/mL, mass concentration are 15mg/mL, quality
Agar that the peptone of a concentration of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes
For 1L, pH 7.
Embodiment 2：
A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing is present embodiments provided, this method includes following step
Suddenly：
(1) pretreatment of raw material：With biological enzyme solutions it is 1 according to mass ratio by banana stalk:3h is impregnated in 5 mixing, after filtering
According to mass ratio it is 1 by filter residue and probiotic solution:2h is impregnated in 5 mixing, and the hot air drying that filter residue is 60 DEG C in temperature is taken after filtering
Air-dried, crushed in dry machine, and by 150 mesh sieve screen, then with mass concentration be 5% Klorvess Liquid according to
Solid-liquid mass ratio is 1:6 mixing, and 12h is impregnated under conditions of temperature is -2 DEG C, it is then placed in the hot air drying that temperature is 60 DEG C
It is air-dried to obtain the banana stalk powder pre-processed in dry machine；
(2) wood vinegar is crude：According to mass ratio it is 55 by the banana stalk powder of step (1) and potassium powder:1 is mixed,
It places into distillation reactor, then nitrogen is passed through in distillation reactor, oxygen is discharged, nitrogen flow 300mL/min；
Distillation reactor is slowly heated to 40 DEG C according to the heating rate of 8 DEG C/min later, later heated at constant temperature, collects distillation reaction
The liquid of cold energy in device obtains crude wood vinegar until banana stalk powder decomposes completely；
(3) wood vinegar is refined：The crude wood vinegar that step (2) obtains is put into molecular distillation reaction kettle and is once divided
The distillate of single flash is carried out quadratic component and distills to obtain acetum pyrolignosum rectificatum liquid by son distillation；Molecular distillation and two
The distillation pressure of secondary molecular distillation is 200Pa, and charging rate is 4mL/min, and rotating speed is 200r/min；Described primary point
The heating temperature of son distillation is 60 DEG C, and the heating temperature of quadratic component distillation is 80 DEG C；
(4) wood vinegar concentrates：According to volume ratio it is 3 by the acetum pyrolignosum rectificatum liquid of step (3) and ether:1 is mixed, and is stood
Layering extracts, and draws ether extraction phase, repeats extraction 8 times, merges ether extraction phase, the heating evaporation removal second in 50 DEG C of water-baths
Ether obtains wood vinegar concentrate I；The NaHCO for being 8% by the acetum pyrolignosum rectificatum liquid of step (3) and mass concentration3It is according to volume ratio
3:1 is mixed, and the pH value of mixed liquor is adjusted to 5 by the NaOH for being again then 5% with mass concentration, and stratification draws extraction
The water layer after acidification is taken, repeats to extract 4 times, combining water layer extract layer, then by extract layer and ethyl acetate solution according to volume ratio
It is 1:3 are mixed, and are drawn ethyl acetate phase removal ethyl acetate and are obtained wood vinegar concentrate II, merge wood vinegar concentrate I
The wood vinegar concentrate is obtained with wood vinegar concentrate II.
Wherein, the biological enzyme solutions of the present embodiment comprise the following components in parts by weight：17 parts of cellulase, 29 parts
Pectase and 26 parts of lignin-degrading enzymes.Wherein, the enzyme activity intensity of cellulase is 900U/g；The enzyme activity intensity of pectase is
1200U/g；The enzyme activity intensity of lignin-degrading enzymes is 1500U/g.
Wherein, the probiotic solution of the present embodiment comprises the following components in parts by weight：Every gram of viable count is 8.7 ×
107CFU/g acetobacter xylinums, every gram of viable count are 5.8 × 108CFU/g saccharomycete and every gram of viable count are 4.5 × 109CFU/g vinegar
Acidfast bacilli.
In order to obtaining more efficient acetobacter xylinum, saccharomycete and acetobacter, need to acetobacter xylinum, saccharomycete and
Acetobacter is tamed.
The acclimation method of above-mentioned acetobacter xylinum is：Acetobacter xylinum is placed in domestication culture medium I and is cultivated, is monitored in culture medium
Content of acetone；Terminate first phase culture when content of acetone reaches 6mg/L in culture medium, and incubation time is denoted as T1；The
One phase carried out second phase culture after cultivating, and picks out the bacterial strain after first phase culture and is placed in the culture of same recipe
Middle culture, and first phase incubation is repeated, terminate second phase culture when the content of acetone in culture medium reaches 6mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/5T1When, complete domestication process；The activation medium
I by as follows at being grouped as：Pectin that cellulose that mass concentration is 100mg/L, mass concentration are 50mg/L, mass concentration are
Yeast extract that chitosan that the lignin of 50mg/L, mass concentration are 5mg/mL, mass concentration are 15mg/mL, mass concentration are
Agar that the peptone of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes are 1L, pH
It is 6.5.
The acclimation method of above-mentioned saccharomycete is that saccharomycete is placed in domestication medium ii to cultivate, and monitors the second in culture medium
Alcohol content；Terminate first phase culture when ethanol content reaches 100mg/L in culture medium, and incubation time is denoted as T1；First
Phase cultivate after carry out second phase culture, pick out the first phase culture after bacterial strain be placed in the culture of same recipe
Culture, and first phase incubation is repeated, terminate second phase culture when the content of ethyl alcohol in culture medium reaches 100mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/2T1When, complete domestication process；The activation medium
II by as follows at being grouped as：Pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are
Glucose that starch that the lignin of 10mg/L, mass concentration are 15mg/L, mass concentration are 100mg/mL, mass concentration are
Agar that peptone that the yeast extract of 15mg/mL, mass concentration are 10mg/mL, mass concentration are 10mg/mL, remaining be banana
Straw juice, culture medium I total volumes are 1L, pH 6.8.
The acclimation method of above-mentioned acetobacter is that acetobacter xylinum is placed in domestication culture medium I to cultivate, and is monitored in culture medium
PH value；Terminate first phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T1；First phase culture terminates
Second phase culture is carried out afterwards, is picked out the bacterial strain after first phase culture and is placed in the culture of same recipe and cultivates, lays equal stress on
Multiple first phase incubation constantly terminates second phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T2, such as
This repeats the n phases and cultivates, and works as Tn=1/3T1When, complete domestication process；The activation medium I by as follows at being grouped as：
Lignin that pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are 10mg/L, quality
Yeast extract that glucose that the ethyl alcohol of a concentration of 200mg/L, mass concentration are 100mg/mL, mass concentration are 15mg/mL, quality
Agar that the peptone of a concentration of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes
For 1L, pH 7.
Embodiment 3：
A kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing is present embodiments provided, this method includes following step
Suddenly：
(1) pretreatment of raw material：With biological enzyme solutions it is 1 according to mass ratio by banana stalk:2.5h, filtering are impregnated in 3 mixing
It is afterwards 1 according to mass ratio by filter residue and probiotic solution:1.5h is impregnated in 4 mixing, and the heat that filter residue is 55 DEG C in temperature is taken after filtering
It air-dried, crushed in wind drying machine, and screened by the sieve of 120 mesh, the Klorvess Liquid for being then 3% with mass concentration
It is 1 according to solid-liquid mass ratio:5 mixing, and 11h is impregnated under conditions of temperature is -4 DEG C, it is then placed in the heat that temperature is 55 DEG C
It is air-dried to obtain the banana stalk powder pre-processed in wind drying machine；
(2) wood vinegar is crude：According to mass ratio it is 52 by the banana stalk powder of step (1) and potassium powder:1 is mixed,
It places into distillation reactor, then nitrogen is passed through in distillation reactor, oxygen is discharged, nitrogen flow 250mL/min；
Distillation reactor is slowly heated to 35 DEG C according to the heating rate of 7 DEG C/min later, later heated at constant temperature, collects distillation reaction
The liquid of cold energy in device obtains crude wood vinegar until banana stalk powder decomposes completely；
(3) wood vinegar is refined：The crude wood vinegar that step (2) obtains is put into molecular distillation reaction kettle and is once divided
The distillate of single flash is carried out quadratic component and distills to obtain acetum pyrolignosum rectificatum liquid by son distillation；Molecular distillation and two
The distillation pressure of secondary molecular distillation is 150Pa, and charging rate is 3.5mL/min, and rotating speed is 150r/min；It is described primary
The heating temperature of molecular distillation is 55 DEG C, and the heating temperature of quadratic component distillation is 75 DEG C；
(4) wood vinegar concentrates：According to volume ratio it is 2.5 by the acetum pyrolignosum rectificatum liquid of step (3) and ether:1 is mixed, quiet
It sets layering to extract, draws ether extraction phase, repeat extraction 7 times, merge ether extraction phase, the heating evaporation removal in 45 DEG C of water-baths
Ether obtains wood vinegar concentrate I；The NaHCO for being 7% by the acetum pyrolignosum rectificatum liquid of step (3) and mass concentration3According to volume ratio
It is 2.5:1 is mixed, and the pH value of mixed liquor is adjusted to 5 by the NaOH for being again then 4% with mass concentration, and stratification is inhaled
Water layer after taking extraction to be acidified repeats to extract 4 times, combining water layer extract layer, then by extract layer and ethyl acetate solution according to body
Product is than being 1:2 are mixed, and are drawn ethyl acetate phase removal ethyl acetate and are obtained wood vinegar concentrate II, merge wood vinegar concentration
Liquid I and wood vinegar concentrate II obtain the wood vinegar concentrate.
Wherein, the biological enzyme solutions of the present embodiment comprise the following components in parts by weight：15 parts of cellulase, 25 parts
Pectase and 21 parts of lignin-degrading enzymes.Wherein, the enzyme activity intensity of cellulase is 800U/g；The enzyme activity intensity of pectase is
1100U/g；The enzyme activity intensity of lignin-degrading enzymes is 1300U/g.
Wherein, the probiotic solution of the present embodiment comprises the following components in parts by weight：Every gram of viable count is 5.7 ×
107CFU/g acetobacter xylinums, every gram of viable count are 4.8 × 108CFU/g saccharomycete and every gram of viable count are 2.5 × 109CFU/g vinegar
Acidfast bacilli.
In order to obtaining more efficient acetobacter xylinum, saccharomycete and acetobacter, need to acetobacter xylinum, saccharomycete and
Acetobacter is tamed.
The acclimation method of above-mentioned acetobacter xylinum is：Acetobacter xylinum is placed in domestication culture medium I and is cultivated, is monitored in culture medium
Content of acetone；Terminate first phase culture when content of acetone reaches 6mg/L in culture medium, and incubation time is denoted as T1；The
One phase carried out second phase culture after cultivating, and picks out the bacterial strain after first phase culture and is placed in the culture of same recipe
Middle culture, and first phase incubation is repeated, terminate second phase culture when the content of acetone in culture medium reaches 6mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/5T1When, complete domestication process；The activation medium
I by as follows at being grouped as：Pectin that cellulose that mass concentration is 100mg/L, mass concentration are 50mg/L, mass concentration are
Yeast extract that chitosan that the lignin of 50mg/L, mass concentration are 5mg/mL, mass concentration are 15mg/mL, mass concentration are
Agar that the peptone of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes are 1L, pH
It is 6.5.
The acclimation method of above-mentioned saccharomycete is that saccharomycete is placed in domestication medium ii to cultivate, and monitors the second in culture medium
Alcohol content；Terminate first phase culture when ethanol content reaches 100mg/L in culture medium, and incubation time is denoted as T1；First
Phase cultivate after carry out second phase culture, pick out the first phase culture after bacterial strain be placed in the culture of same recipe
Culture, and first phase incubation is repeated, terminate second phase culture when the content of ethyl alcohol in culture medium reaches 100mg/L, and
Incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/2T1When, complete domestication process；The activation medium
II by as follows at being grouped as：Pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are
Glucose that starch that the lignin of 10mg/L, mass concentration are 15mg/L, mass concentration are 100mg/mL, mass concentration are
Agar that peptone that the yeast extract of 15mg/mL, mass concentration are 10mg/mL, mass concentration are 10mg/mL, remaining be banana
Straw juice, culture medium I total volumes are 1L, pH 6.8.
The acclimation method of above-mentioned acetobacter is that acetobacter xylinum is placed in domestication culture medium I to cultivate, and is monitored in culture medium
PH value；Terminate first phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T1；First phase culture terminates
Second phase culture is carried out afterwards, is picked out the bacterial strain after first phase culture and is placed in the culture of same recipe and cultivates, lays equal stress on
Multiple first phase incubation constantly terminates second phase culture when pH in culture medium value reaches 3, and incubation time is denoted as T2, such as
This repeats the n phases and cultivates, and works as Tn=1/3T1When, complete domestication process；The activation medium I by as follows at being grouped as：
Lignin that pectin that cellulose that mass concentration is 20mg/L, mass concentration are 10mg/L, mass concentration are 10mg/L, quality
Yeast extract that glucose that the ethyl alcohol of a concentration of 200mg/L, mass concentration are 100mg/mL, mass concentration are 15mg/mL, quality
Agar that the peptone of a concentration of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes
For 1L, pH 7.
Control group 1：
This control group pre-processes banana fiber without using biological enzyme, other parameters, method and embodiment 1 complete one
It causes.
Control group 2：
This control group pre-processes banana fiber without using probiotics, other parameters, method and embodiment 1 complete one
It causes.
Control group 3：
Without domestication, other parameters, method and implementation when the probiotics of this control group pre-processes banana fiber
Example 1 is completely the same.
Control group 4：
This control group pre-processes banana fiber without using biological enzyme and probiotics, other parameters, method and implementation
Example 1 is completely the same.
Testing experiment 1：
Each component content of concentration wood vinegar of testing example 1-3 and control group 1-4, the results are shown in Table 1：
1 unit of table：%
As seen from the above table, the acids of embodiment 1-3 and esters content are significantly greater than control group 1-4；The hydrazine of embodiment 1-3 contains
Amount is significantly lower than control group 1-4, and the hydrazine content of embodiment 1-3 is examined substantially not to be measured, and illustrates the biological enzyme Jing Guo the application and benefit
Raw bacterium solution treatment can significantly improve the acid of banana wood vinegar and the content of ester, reduce the content of hydrazine.
Testing experiment 2：
The wood vinegar yield of testing example 1-3 and control group 1-4, the results are shown in Table 2：
Table 2
| Test item | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group 1 | Control group 2 | Control group 3 | Control group 4 |
| Wood vinegar yield (%) | 82.36 | 81.65 | 81.64 | 53.25 | 56.26 | 50.26 | 33.21 |
As seen from the above table, the wood vinegar yield of embodiment 1-3 is apparently higher than control group 1-4, illustrates the biological enzyme of the application
Solution and probiotic solution can significantly improve wood vinegar yield, while probiotics can significantly improve wood vinegar production after domestication
Amount.
Testing experiment 3：
Fresh-keeping experiment：Antistaling agent test group is matched according to following test group：
Test group 1：According to volume ratio it is 1 by the concentration wood vinegar of embodiment 1 and water:5 are mixed to prepare antistaling agent；
Test group 2：According to volume ratio it is 1 by the concentration wood vinegar of embodiment 2 and water:5 are mixed to prepare antistaling agent；
Test group 3：According to volume ratio it is 1 by the concentration wood vinegar of embodiment 3 and water:5 are mixed to prepare antistaling agent；
Test group 4：According to volume ratio it is 1 by the concentration wood vinegar of control group 1 and water:5 are mixed to prepare antistaling agent；
Test group 5：According to volume ratio it is 1 by the concentration wood vinegar of control group 2 and water:5 are mixed to prepare antistaling agent；
Test group 6：According to volume ratio it is 1 by the concentration wood vinegar of control group 3 and water:5 are mixed to prepare antistaling agent；
Test group 7：According to volume ratio it is 1 by the concentration wood vinegar of control group 4 and water:5 are mixed to prepare antistaling agent；
Control group：Pure water
Fresh-keeping experiment：It is immersed in test group 1-7 by equivalent amount, with the fresh apple of batch, banana, Chinese cabbage, romaine lettuce
Antistaling agent and control group in, impregnate to take out after 2h and be placed on and dry at room temperature, and wrap up one layer of preservative film, observe and test each examination
Test that group fruit surface starts the number of days for blackspot occur and vegetables start to occur rotting the number of days of water outlet phenomenon.Specifically it is shown in Table 3：
Table 3
| Group | Apple | Banana | Chinese cabbage | Romaine lettuce |
| Test group 1 | 45d | 21d | 25d | 20d |
| Test group 2 | 43d | 23d | 26d | 21d |
| Test group 3 | 44d | 20d | 24d | 23d |
| Test group 4 | 21d | 13d | 12d | 11d |
| Test group 5 | 23d | 14d | 14d | 10d |
| Test group 6 | 22d | 12d | 14d | 12d |
| Test group 7 | 23d | 15d | 13d | 11d |
| Control group | 5d | 2d | 3d | 2d |
As seen from the above table, test group 1-3 apples, banana, Chinese cabbage, romaine lettuce fresh keeping time be apparently higher than test group 4-7,
The fresh keeping time of test group 4-7 is apparently higher than control group；Illustrate that wood vinegar can play the role of fruit freshness preserving, and uses this Shen
The wood vinegar fresh-keeping effect of method production please is more preferably.
Toxicity test：
By equivalent amount, with batch new fresh bananas with the antistaling agent of test group 1-7 and control group impregnate 2h after, directly general
Banana skin, banana pulp feed mouse, and every group of banana feeds 20 mouse, wherein 10 feed banana skin, 10 fillings
Banana pulp is fed, every mouse feeding amount is 100g/ days；Continuous feeding (all repeats antistaling agent to impregnate, feed step for 10 days daily
Suddenly after), the activity condition of mouse is observed, and slaughters mouse, measures the organic components and residual condition of mouse liver, specific feelings
Condition is shown in Table 4：
Table 4
As seen from the above table, the wood vinegar of control group 1-4 productions can cause have a small amount of hydrazine residual in Mice Body in banana skin
Stay, and using the present processes production Banana fresh-keeping agent epidermis and pulp will not all cause to have in Mice Body it is any it is organic at
Divide residual, it can be seen that, the wood vinegar antistaling agent of the application is safely and effectively nontoxic.
In conclusion preparing wood vinegar using banana stalk produced by the invention has good preserving fruit and vegetable utilizing effect, together
When, the residual of harmful substance will not be also caused, is a kind of safe and effective antistaling agent.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of banana wood vinegar preparation method applied to preserving fruit and vegetable utilizing, which is characterized in that described method includes following steps：
(1) pretreatment of raw material：With biological enzyme solutions it is 1 according to mass ratio by banana stalk:2h-3h is impregnated in 2-5 mixing, after filtering
According to mass ratio it is 1 by filter residue and probiotic solution:2-5 mixing impregnate 1h-2h, taken after filtering filter residue temperature be 50 DEG C -60
DEG C air drier in air-dried, crushed, and screened by the sieve of -150 mesh of 100 mesh, be then with mass concentration
The Klorvess Liquid of 2%-5% is 1 according to solid-liquid mass ratio:4-6 is mixed, and is impregnated under conditions of temperature is -5 DEG C~-2 DEG C
10h-12h is then placed in the air drier that temperature is 50 DEG C -60 DEG C and is air-dried to obtain the banana straw powder pre-processed
End；
(2) wood vinegar is crude：According to mass ratio it is 50-55 by the banana stalk powder of step (1) and potassium powder:1 is mixed, then
It is put into distillation reactor, then nitrogen is passed through in distillation reactor, oxygen is discharged, nitrogen flow 200-300mL/
min；Distillation reactor is slowly heated to 30 DEG C -40 DEG C according to the heating rate of 5 DEG C/min-8 DEG C/min later, later constant temperature
The liquid of cold energy in distillation reactor is collected in heating, until banana stalk powder decomposes completely, obtains crude wood vinegar；
(3) wood vinegar is refined：The crude wood vinegar that step (2) obtains is put into molecular distillation reaction kettle and carries out a molecule steaming
It evaporates, the distillate of single flash, which is carried out quadratic component, to be distilled to obtain acetum pyrolignosum rectificatum liquid；Molecular distillation and secondary point
The distillation pressure of son distillation is 100-200Pa, and charging rate is 3-4mL/min, and rotating speed is 100-200r/min；It is described
The heating temperature of molecular distillation is 50-60 DEG C, and the heating temperature of quadratic component distillation is 70-80 DEG C；
(4) wood vinegar concentrates：According to volume ratio it is 2-3 by the acetum pyrolignosum rectificatum liquid of step (3) and ether:1 is mixed, and is stood and is divided
Layer extraction, draws ether extraction phase, repeats extraction 6-8 time, merging ether extraction phase, heating evaporation is gone in 40-50 DEG C of water-bath
Except ether obtains wood vinegar concentrate I；It is the NaHCO of 5%-8% by the acetum pyrolignosum rectificatum liquid of step (3) and mass concentration3According to
Volume ratio is 2-3:1 is mixed, and the pH value of mixed liquor is adjusted to 5 by the NaOH for being again then 3%-5% with mass concentration, quiet
It sets layering, draws the water layer after extraction acidification, repeat extraction 3-4 times, combining water layer extract layer, then by extract layer and ethyl acetate
Solution is 1 according to volume ratio:1-3 is mixed, and is drawn ethyl acetate phase removal ethyl acetate and is obtained wood vinegar concentrate II, closes
And wood vinegar concentrate I and wood vinegar concentrate II obtain the wood vinegar concentrate.
2. banana wood vinegar preparation method according to claim 1, which is characterized in that the biological enzyme of the step (1) is molten
Liquid comprises the following components in parts by weight：12 parts -17 parts of cellulase, 21 parts -29 parts of pectase and 17 parts -26 parts of wood
Lignin-degrading enzymes.
3. banana wood vinegar preparation method according to claim 2, which is characterized in that the enzyme activity intensity of the cellulase
For 700U/g-900U/g；The enzyme activity intensity of pectase is 1000U/g-1200U/g；The enzyme activity intensity of lignin-degrading enzymes is
1200U/g-1500U/g。
4. banana wood vinegar preparation method according to claim 1, which is characterized in that the probiotics of the step (1) is molten
Liquid comprises the following components in parts by weight：Every gram of viable count is 1.6 × 107CFU/g~8.7 × 107CFU/g acetobacter xylinums, every gram
Viable count is 2.3 × 108CFU/g~5.8 × 108CFU/g saccharomycete and every gram of viable count are 1.8 × 109CFU/g~4.5 ×
109CFU/g acetobacters.
5. banana wood vinegar preparation method according to claim 3, which is characterized in that the acclimation method of the acetobacter xylinum
For：Acetobacter xylinum is placed in domestication culture medium I and is cultivated, the content of acetone in culture medium is monitored；When content of acetone in culture medium
Terminate the first phase culture when reaching 6mg/L, and incubation time is denoted as T1；The first phase carries out second phase culture after cultivating,
It picks out the bacterial strain after first phase culture to be placed in the culture of same recipe and cultivate, and repeats first phase incubation,
Terminate second phase culture when the content of acetone in culture medium reaches 6mg/L, and incubation time is denoted as T2, so repeat
The n phases cultivate, and work as Tn=1/5T1When, complete domestication process；The activation medium I by as follows at being grouped as：Mass concentration is
Lignin that pectin that the cellulose of 100mg/L, mass concentration are 50mg/L, mass concentration are 50mg/L, mass concentration are
Peptone that yeast extract that the chitosan of 5mg/mL, mass concentration are 15mg/mL, mass concentration are 10mg/mL, mass concentration are
The agar of 10mg/mL, remaining be banana straw juice, culture medium I total volumes be 1L, pH 6.5.
6. banana wood vinegar preparation method according to claim 3, which is characterized in that the acclimation method of the saccharomycete is
Saccharomycete is placed in domestication medium ii and is cultivated, the ethanol content in culture medium is monitored；When ethanol content reaches in culture medium
Terminate the first phase culture when 100mg/L, and incubation time is denoted as T1；The first phase carries out second phase culture after cultivating, and chooses
It selects the bacterial strain after first phase culture to be placed in the culture of same recipe and cultivate, and repeats first phase incubation, when
Terminate the second phase culture when content of ethyl alcohol reaches 100mg/L in culture medium, and incubation time is denoted as T2, so repeat
The n phases cultivate, and work as Tn=1/2T1When, complete domestication process；The activation medium II by as follows at being grouped as：Mass concentration
For the cellulose of 20mg/L, mass concentration be 10mg/L pectin, mass concentration be 10mg/L lignin, mass concentration be
Yeast extract that glucose that the starch of 15mg/L, mass concentration are 100mg/mL, mass concentration are 15mg/mL, mass concentration are
Agar that the peptone of 10mg/mL, mass concentration are 10mg/mL, remaining be banana straw juice, culture medium I total volumes are 1L, pH
It is 6.8.
7. banana wood vinegar preparation method according to claim 3, which is characterized in that the acclimation method of the acetobacter
It is cultivated for acetobacter xylinum to be placed in domestication culture medium I, monitors the pH value in culture medium；It is tied when pH in culture medium value reaches 3
The beam first phase cultivates, and incubation time is denoted as T1；The first phase carries out second phase culture after cultivating, and picks out first phase training
Bacterial strain after supporting, which is placed in the culture of same recipe, to be cultivated, and repeats first phase incubation, when pH in culture medium value
Reach 3 and constantly terminate second phase culture, and incubation time is denoted as T2, so repeating the n phases cultivates, and works as Tn=1/3T1When,
Complete domestication process；The activation medium I by as follows at being grouped as：Mass concentration is dense for the cellulose of 20mg/L, quality
Spending ethyl alcohol, mass concentration that the pectin for being 10mg/L, the lignin that mass concentration is 10mg/L, mass concentration are 200mg/L is
Peptone that yeast extract that the glucose of 100mg/mL, mass concentration are 15mg/mL, mass concentration are 10mg/mL, mass concentration
For 10mg/mL agar, remaining be banana straw juice, culture medium I total volumes are 1L, pH 7.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011355A (en) * | 2020-09-21 | 2020-12-01 | 阜阳师范大学 | Wood vinegar refining and purifying method |
| CN115678063A (en) * | 2022-10-08 | 2023-02-03 | 吉林大学 | Efficient antibacterial polymer packaging film |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112011355A (en) * | 2020-09-21 | 2020-12-01 | 阜阳师范大学 | Wood vinegar refining and purifying method |
| CN115678063A (en) * | 2022-10-08 | 2023-02-03 | 吉林大学 | Efficient antibacterial polymer packaging film |
| CN115678063B (en) * | 2022-10-08 | 2024-03-15 | 吉林大学 | Efficient antibacterial polymer packaging film |
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