CN108850659A - A kind of laying cycle of laying hens feed and preparation method thereof extending egg shelf life - Google Patents
A kind of laying cycle of laying hens feed and preparation method thereof extending egg shelf life Download PDFInfo
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- CN108850659A CN108850659A CN201811149268.6A CN201811149268A CN108850659A CN 108850659 A CN108850659 A CN 108850659A CN 201811149268 A CN201811149268 A CN 201811149268A CN 108850659 A CN108850659 A CN 108850659A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention belongs to fodder compound technical fields, and in particular to a kind of laying cycle of laying hens feed and preparation method for extending egg shelf life, and be related to feeding the long shelf life laying high selenium eggs that the feed obtains.The feed, raw material include 20-35 parts of corn, 5-8 parts of dregs of beans, 2-5 parts of expanded soybean, 3-8 parts of extruding linseed, 3-8 parts of corn protein powder, 2-4 parts of sesame seed meal, 3-8 parts of rice bran, 1-3 parts of hot chili residue, 0.5-1.0 parts of palm-kernel oil, 1-5 parts of malt root, 0.8-2 parts of biological fermentation feed, it is excellent can 0.03-0.05 parts of treasured, 0.025 part of yeast selenium etc..And biological fermentation feed is prepared using bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici, saccharomyces cerevisiae mixed fermentation.Feed of the present invention is used for breeding layer chicken, Se content in egg can be made to be higher than every piece of egg of 22.85ug/, up to 80 days, normal-temperature shelf life reached 50 days shelf life at 4-8 DEG C.
Description
Technical field
The invention belongs to fodder compound technical fields, and in particular to a kind of laying cycle of laying hens feeding for extending egg shelf life
Material, and it is related to the preparation method of the feed, and feed the long shelf life laying high selenium eggs that the feed obtains.
Background technique
Egg is high-quality protein original, protein, vitamin B complex and microelement rich in, in yolk containing DHA,
Lecithin and lutein etc., human absorptivity reaches 98% or more, has brain tonic and intelligence development, protection liver, anti-aging etc. edible
Effect;The concept and standard of egg shelf life:Shelf life refers to that product can be received and be met the time of customer's quality requirement
Length.When food is after storage after a period of time, its one or more of metric attributes have reached undesirable state, this
Its unsuitable is put on shelf is sold under state, the exactly so-called shelf life for reaching this kind of product.Egg shelf life
Just refer to that fresh egg eventually arrives in consumer's hand be able to maintain quality not by the intermediate links such as transporting, selling from after production packaging
The time of change.In the U.S., egg shelf life was generally limited within 45 days, and was required egg in disinfection, transported, sold
It all must be stored in 7 DEG C or less in journey.The domestic standard for not yet formulating egg shelf life.The index for measuring egg shelf life is usual
There are Hough unit, air room height, mass loss rate, yolk coefficient and content of microorganisms etc..Egg leaves certainly as living beings
After parent, its inside quality is gradually decreased.Caused by the variation of egg quality includes physical change, chemical change, microorganism
Variation etc..As storage time extends, freshly-slaughtered poultry egg quality, Hough unit, TVB-N content etc. are in significant negative correlation (P<
0.01), (P is positively correlated in significant with gas chamber variation<0.01).Chen Jianfeng etc. has studied egg under the conditions of different reserve temperatures
The variation of egg quality index after storage 30 days, including gas chamber depth, yolk index, dense albumen content, Volatile Base Nitrogen
Content etc..Liu Huizhen etc. has studied the correlation of egg quality and period of storage under room temperature.It is stored at room temperature 21 days and deepfreeze
At 56 days, the dense albumen of a small amount of egg aquation under the action of enzyme is had, causes Kazakhstan value that cannot measure.Mass loss rate is chicken
Mass loss ratio of the egg before and after storage is the important indicator for measuring egg quality economic value.The mass loss rate meeting of egg
Accelerate to become larger with the raising of reserve temperature.When 25 DEG C of conditions are stored 27 days, egg mass loss rate is 11.22%, is not had
Wrapped egg mass loss rate after (25 ± 1) DEG C condition is stored 28 days reaches 10%, non-film and atoleine film
Mass loss rate is respectively 11% and 9.2% after egg room temperature storage 28 days;Rocculi et al. studies 25 DEG C of condition controlled atmospheric packings
Brown shell egg, showing that unpacked egg stores 28 days mass loss rates in 25 DEG C of conditions is 6%.What different researchers obtained
Egg mass loss rate is not both because the factors such as storage temperature and humidity conditions, egg size, shell thickness and stomata are how many cause
's.Yolk coefficient is the ratio of yolk height and yolk diameter, and domestic and international multiple scholars use this Indexs measure egg
Inside quality.Zhang Yuhua et al. research shows that with storage time extension, yolk coefficient is on a declining curve, and cryopreservation
Egg yolk coefficient fall is significantly less than the egg of room temperature storage.Chen Jian cutting edge of a knife or a sword et al. passes through the measurement to egg quality index
It was found that 8 DEG C of egg yolk indexes after storage 30 days are 0.294, egg is without obvious corrupt feature, but when storing under the conditions of 20 DEG C
Yolk index is only 0.212, and has obvious putrefactive odor.Egg system high-moisture, high nutrition component food are easy in storage
In caused putrid and deteriorated by harmful microbial contamination.Here microorganism is mainly bacterium, is divided into eggshell surface and egg
The two types in portion mainly have Escherichia coli, salmonella etc..The egg eggshell surface of the researchs such as Liu Junmei discovery different brands
In storage initial stage total number of bacteria between 9.315 × 102-1.367×104CFU/, and as the extension of period of storage is in rise
Gesture.The total plate count of egg shell and content in summer under room temperature as the increase of storage time is in rising trend, chicken
The coliform sum of egg egg white and egg yolk mixture was continuously increased from the 8th day with the extension of time, and the 25th day egg is
It is completely rotten.Temperature is high, and when the holding time is long, the recall rate of salmonella is just high in egg, 3 days Salmonellas of general 25 DEG C of storages
Bacterium can penetrate eggshell and shell membrane, infect egg content.National situations are to reach supermarket from egg production date and market is big at present
About need 7-10 days time, consumer store general 1 month even more than time.
A large amount of data at home and abroad has been consulted at present, and the report in terms of discovery feed nutrition and shelf life correlative study is very
It is few.
Publication number:The invention of 104397362A《Rosemary grass meal preparation process and its application in egg feedstuff》This hair
It is bright to disclose a kind of rosemary grass meal preparation process and its application in egg feedstuff, it air-dries and is lower than by using refrigeration
0 DEG C of ultra micro Freezing smashing processing technology farthest makes to discharge the bioactive substance in rosemary blade, as feed
Additive is used for high-yield egg chicken, improves the egg production and Egg Quality of laying hen, which declares above-mentioned when adding in egg feedstuff
The rosemary grass meal 0.3-0.9% of technique production has when especially implementing addition 0.3% and significantly improves laying rate, reduces feed
Consumption improves Egg Quality, extends the effect of egg shelf life.But by the disclosure of which from the point of view of, the invention egg product freshness with
The fresh hen egg ratio of market sale does not have advantage, and Hough unit is low, only between 78.06-83.83, illustrates relevant albumen height
Also low, egg water content is high, and shelf life accordingly can be short;And rosemary grass meal is not The Ministry of Agriculture of the People's Republic of China, MOA's bulletin the
No. 1773 files《Feedstuff catalogue》In raw material, do not allow to use in feed;The raw material source of goods is few, and raw material process is unclear
Chu is difficult in feed.
Publication number:The invention of 105124244A《A kind of comprehensive utilization coconut husk preparation increase egg production feed addictive of layer chicken》
A kind of comprehensive utilization coconut husk preparation increase egg production feed addictive of layer chicken is disclosed, is made from the following raw materials in parts by weight:Eggplant
It is sub- 20-25, Semen Coicis 13-16, rape 10-12, sesame paste 6-7, coffee grounds 4-5, lard 2-3, tangerine peel 15-17, cloves 9-12, sweet
Careless 6-7, cocoanut shell, lactic acid bacteria, the hydrochloric acid of 0.2mol/L, 60% ethyl alcohol, acetone, appropriate amount of water.The invention shows that egg can be improved
Egg production, Egg Quality and the shelf life for extending egg of chicken, significantly improve egg feedstuff remuneration and egg laying performance.The invention
Coconut husk is not that The Ministry of Agriculture of the People's Republic of China, MOA announces No. 2045《Catalogue of feed additive varieties (2013)》In raw material add
Add agent, nor The Ministry of Agriculture of the People's Republic of China, MOA announces No. 1773 file《Feedstuff catalogue》In raw material, Bu Nengying
For in feed;Single additive is extremely difficult to the target of Shelf-life in feed, and the goal of the invention of the patent is only
It is to improve egg production, it is different from research purpose of the invention.
Publication number:The invention of 06360042A《A kind of laying cycle of laying hens premixed feed》Disclose a kind of Egg Production of Laying Hens
Phase premixed feed includes following components:15-23 parts of vitamin, 45-60 parts of amino acid, adds 40-56.5 parts of microelement
Add 44.5-59 parts and feed vector 855.5-1050 parts of agent.The present invention is mixed and made into mixed feed with corn, dregs of beans, mountain flour,
Specialize in laying cycle of laying hens use.The special designing of vitamin of the present invention keeps the nutrition of egg more balanced, and storage, which is unlikely to deteriorate, to be made
Shelf life is longer;Laying hen shin bone zinc deposition and intestinal mucosa zinc deposition and immunity of organisms can be improved in organic zinc, manganese;Organic micro member
Element and inorganic salts cooperation addition can increase shell thickness.It is rich in DHA in feed simultaneously, is converted into rich in lecithin form DHA's
Egg, the utilization of no fishy smell and the more conducively person.But since egg shelf life is determined by the Egg Quality of fresh hen egg, and chicken
The Egg Quality integrated system of egg is nutritionally relevant, such as anti-oxidant nutrition, raising premunition nutrition, raising albumen concentration nutrition,
Therefore the invention is extremely difficult to extend the purpose of egg shelf life using single vitamin special designing.
The optimal proportion of Australia's health and ω -6/ ω -3 PUFA in the diet of medical commission referrer in 2006
For (2-4):1.It is 4 that present Japanese health ministry, which recommends ω -6/ ω -3 mean ratio,:1, and China yet there are no related report.
Content of fatty acid is high in egg, and the scrambled egg product rich in omega 6 polyunsaturated fatty acid is oxidizable, and shelf life is short.
Fatty acid oxidation be mainly unsaturated fatty acid oxidation caused by adipose tissue it is rancid, cause the nutritive value of egg to damage
Mistake, organoleptic attribute reduce and are formed potential noxious material, and then influence egg quality and reduce shelf life.Xia Lunzhi (2016)
Following viewpoint is disclosed in the 12nd Animal nutrition branch:Polyunsaturated fatty acid is easy to oxidize, and saturated fat acid-proof is not
It is oxidized easily;Due to using the raw material sources such as rice bran oil, corn oil, lard in conventional egg feedstuff, and fat composition is beautiful
Rice provide 60% or more it is adipose-derived, be difficult to reduce ω -6/ ω -3 ratio, ω -6/ ω -3 is most in egg at present
It is distributed in 10-20:Oxidizable between 1, egg is perishable.Alpha-tocopherol (vitamin E) is added in diet can be improved egg
Inoxidizability, alpha-tocopherol inoxidizability with meat organoleptic attribute by grease in its additive amount in diet, diet unsaturation
Property and oxidisability, animal physiological state and meat in net oxidation balance influence.VE, which has, interrupts lipid oxidation chain reaction, removes
Free radical controls the sensibility of meat lipid oxidation, improves the effect of meat.VE can protect fat from oxidation as antioxidant
Destroy (Tappel, 1962), can especially protect in biological film phospholipid and plasma protein polyunsaturated fatty acid (Barton etc.,
1993).The same organic peroxy group reaction of the phenolic hydroxyl group of Tocopherol, generates stable fatty hydroperoxidation hydrogen and tocopherol
Peroxy radicals (VE free radical) protect fat from the attack of free radical to effectively interrupt peroxidating chain.Vitamin at present
E price is high, and usage amount is lower than 20 mg/kgs in commodity egg feed, and synthesising complex E when by oxidative stress very
It is easy to be utilized, deposition substantially reduces in egg.When that stress occur with disease, vitamin E is utilized quickly, therefore conventional feeding
Material shows very weak in terms of anti-oxidation stress.
Shen Huiqin etc. (2001) is pointed out in the research to big white mouse membrane structure stability, under low oxygen partial pressure, β-
Carrotene is a kind of special chain breaking antioxidants, can form Stable Carbon core free radical with peroxy radical irreversible reaction, this
A carbon core free radical can form rapidly a kind of new peroxylradicals with oxygen reversible reaction.Since carbon core free radical is quite steady
Fixed, when decrease in oxygen partial pressure, reaction can be sufficiently mobile to carbon core free radical direction, and oxyradical concentration, low work is effectively reduced
The carbon core free radical of property can be reacted with another peroxylradicals and be removed.Fatty acid oxidation stability study in yolk, mistake
For oxidation index (PI) as one of fatty acid peroxidase neurological susceptibility index is measured, the recommendations such as Cortinas (2003) are as follows:PI=
(monoenoic fatty acid content × 0.025)+diene content of fatty acid × 1)+(triethenoid fatty acid content × 2)+(tetraene fatty acid contains
Amount × 4)+(pentaene content of fatty acid × 6)+(six alkene content of fatty acid × 8).It is higher that higher PI value will lead to fatty acid oxidation
Neurological susceptibility.
Diet Factors will lead to oxidative stress, such as presence, content of polyunsaturated fatty acid height and the ratio of mycotoxin
Unbalance, oxidative rancidity consaturated oil, minerals antagonism, the vitamin for adding low amounts etc. will lead to oxidative stress.
The brand egg shelf life of existing market sale is denoted as 40 days, but in many cases, buy 20-30 days chickens
It is thin that egg is just stained with shell, scattered Huang, egg white;How under the premise of guaranteeing egg nutrient, fatty acid oxidation degree is reduced, extends egg
Shelf life is problem to be solved by this invention.
Summary of the invention
Bioaccumulation efficiency of the various nutrients in egg is high in feed, by changing trophic factors combination, reduces egg rouge
Fat acid oxidase degree is feasible.Present invention mainly solves be that egg is easy to dissipate the factors such as yellow, rotten and causes shelf life short
Problem, using system trophism theory, in terms of nutrition means, raising premunition, improving the feed nutritions such as intestinal health state
System solves egg product Geological Problems, improves nutrient maximum digestibility and deposition, enhances antioxygenic property, make Enriching Selenium in egg,
The multiple Antioxidative Factors such as vitamin E, beta carotene, balanced fatty acid reach the target for extending egg shelf life.The present invention
Solve following problems:First is that enhancing feed anti-oxidation function, makes to maximize enrichment Antioxidative Factors in egg, extends egg and become
The time of matter;Second is that promoting intestinal mucosa function and avoiding intestinal tract injury, premunition is improved, improves nutrient maximum digestibility and heavy
Product rate;Third is that scientific and reasonable ω -6/ ω -3 PUFA Proportionality design, reaches the optimal deposition amount in egg;Fourth is that scientific and reasonable set
Ideal amino acid nutrition is counted, albumen concentration and Hough unit are effectively improved, reduces egg water content, produces high-quality, safe egg product.
Solves the nutrition problem of above 4 aspects, so that it may various raw materials and nutrient be compounded by science, preparation extends egg goods
The laying cycle of laying hens feed of frame phase.
In order to achieve the above object, the present invention uses following technical scheme:
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
20-35 parts of corn, 15-30 parts of barley, 5-8 parts of dregs of beans, 2-5 parts of expanded soybean, 3-8 parts of extruding linseed, corn
3-8 parts of albumen powder, 2-4 parts of sesame seed meal, 3-8 parts of rice bran, 1-3 parts of hot chili residue, 0.5-1.0 parts of palm-kernel oil, 1-5 parts of malt root,
0.8-2 parts of biological fermentation feed, it is excellent can 0.03-0.05 parts of treasured, 0.025 part of yeast selenium, 0.01-0.02 parts of vitamin E, β-Hu Luo
0.02-0.04 parts of Bu Su, 0.05-0.1 parts of natural carotenol, 0.45-0.67 parts of amino acid, 0.01-0.015 parts of zinc albuminate, albumen
0.01-0.015 parts of manganese, 10,000 0.01-0.02 parts of unit phytases, 0.03-0.05 parts of choline chloride, glycine betaine 0.03-0.08
Part, 0.05-0.1 parts of complex enzyme formulation, 8-10 parts of mountain flour, 0.5-0.8 parts of calcium monohydrogen phosphate, 0.3-0.4 parts of salt, compound premix
0.5 part.
Further, the laying cycle of laying hens feed, it is 3.2- that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,
4.0:1。
Further, the amino acid is at least two in lysine, methionine, threonine or tryptophan;
Preferably, the amino acid be lysine, methionine, threonine and tryptophan in mass ratio, lysine:Egg ammonia
Acid:Threonine:Tryptophan=1:0.5-0.6:0.3-0.61:0.11-0.15 is uniformly mixed.
Further, the compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-
2012, product authentication code is that pre- word (2013) 003024 is raised by the Liao Dynasty;Product form is multi-vitamins and microelement, and trade mark is
Standing grain is rich, commercially available.Compound premix composition is as follows:Iron 15000mg/Kg, copper 3000mg/Kg, zinc 20000mg/Kg, manganese
24000mg/Kg, selenium 60mg/Kg, iodine 100mg/Kg, vitamin A 1600KIU, vitamin D 600KIU, vitamin E 320mg/
Kg, vitamin K3200mg/Kg, vitamin B1200mg/Kg, vitamin B2880mg/Kg, vitamin B6600mg/Kg, dimension
Raw element B122.0mg/Kg, niacin 6000mg/Kg, pantothenic acid 800mg/Kg, folic acid 100.0mg/Kg, biotin 5.5mg/Kg;
Further, the 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence
Number for raise add (2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;Main component is phytase;It is commercially available;
Further, the complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise
Add (2011) 1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;Contents of Main Components is as follows:Zytase 3000U/
G, mannase 800U/g, acid protease 2000U/g, lipase 800U/g, amylase 300U/g, dextranase 400U/
g;It is commercially available;
Further, it is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;Product effective component:
Hydrolysable Tannins acid 75%;Main function:Natural anti-oxidation characteristic, convergence control diarrhea;
Further, the biological fermentation feed, main active are bacillus coagulans, lactobacillus plantarum, lactic acid
Piece coccus, saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, the thick egg of finished product
White to be more than or equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:2-4:The mass ratio of 5-7
It is mixed with dregs of beans, rice bran meal, and the Mixed Microbes of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added
Liquid, the mixing quality ratio of above-mentioned four kinds of bacterium solutions are 1:1-2:1-3:The mass ratio of 1-5, bacterium solution total amount and fermentation substrate is 1:
2-4;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 3-7 days, and temperature is 30-40 DEG C, and material pH value drops to 4.0-5.5, bacillus coagulans, lactic acid bacteria and saccharomycete
Content, which is respectively greater than, is equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
The preservation of bacteria strain is purchased from China General Microbiological culture presevation administrative center, and strain properties are as follows:
Bacillus coagulans CGMCC No.1.3220:Gram-positive, rod-short produce gemma, in tryptone agar
For white protrusion bacterium colony, 30-37 degrees Celsius of well-grown, degradable macro-molecular protein generates small peptide active material;
Lactobacillus plantarum CGMCC No.1.3:Gram-positive, it is rod-shaped, gemma is not produced, on lactic acid bacteria agar culture medium
For milky protrusion bacterium colony, 30-37 degrees Celsius of well-grown generates the metabolites such as lactic acid using oligosaccharides;
Pediococcus acidilactici CGMCC No.1.2696:Gram-positive, it is spherical, gemma is not produced, in lactic acid bacteria agar culture medium
Upper is milky protrusion bacterium colony, and 30-37 degrees Celsius of well-grown generates the metabolites such as lactic acid using oligosaccharides;
Saccharomyces cerevisiae CGMCC No.2.1792:Fungi is white circular edge on potato dextrose agar
Smooth protrusion bacterium colony, 25-30 degrees Celsius of well-grown generate the metabolites such as alcohol, solid culture using glucose
With apparent fragranced.
Present invention simultaneously provides a kind of preparation methods of laying cycle of laying hens feed for extending egg shelf life, including following step
Suddenly:
Each component raw material is accurately weighed according to formula, is crushed according to conventional laying hen processing method, then in parts by weight
It is arrogant to small to sequentially add various raw materials, it is uniformly mixed, processes and raised to get a kind of laying cycle of laying hens for extending egg shelf life
Material.
Present invention simultaneously provides a kind of laying high selenium eggs of long shelf life, Se content is higher than every piece of 22.85ug/ in the egg
Egg, 4-8 DEG C of shelf life can reach 80 days, and shelf life reaches 50 days at 20-25 DEG C of egg room temperature.
The production method of the laying high selenium eggs includes the following steps:In laying cycle of laying hens, prolong described in feeding according to a conventional method
The laying cycle of laying hens feed of long egg shelf life.
Beneficial effect
The present invention uses system trophism theory, by reinforcing nutrition, does not need to solve emphatically using physically or chemically means
Egg caused by various problem bring oxidative stress of having determined is easy to dissipate the problem that the factors such as yellow, rotten cause shelf life short, shows
Extend the shelf life of egg with writing.The egg of Shelf-life is of great significance:First is that realizing egg product safety and providing excellent
Matter egg product, shelf life is longer to show that egg quality is better;Second is that supermarket does not need to increase cost, when can extend egg undercarriage
Between, it can be improved economic benefit, reduce waste;Livestock products can be traced system and carry out quickly, for aquaculture, intermediate links, weight
It is extremely urgent depending on egg product safety problem;Third is that strengthening the anti-oxidant nutrition of egg, do not need egg in preservation and transport
The fresh-keeping method of mineral oil is applied using traditional, is ensured food safety from feed nutrition angle.The formation of free radicals in vivo
There is a kind of natural equilibriums between animal endogenous anti-oxidative ability.The reactive oxygen that free basal cell's eubolism generates
Change metabolin (ROM) animal endogenous anti-oxidative ability and is mainly made of (Miiler et al, 1993) the three classes antioxidant
One kind is antioxidase, with superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) for representative;They are
Resist the ROM such as oxide (ROO-), hydroperoxides first of intracellular main anti-oxidative defense system and collective is anti-
Imperial system.Glutathione peroxidase (GSH-Px) is selenium dependence.Second class is resisted by the protein in intracellular fluid
Oxidant, such as the albumin with sulfydryl, cysteine and homocysteine.Third class is that the chain blocking type of small molecule is anti-oxidant
Agent, such as water-soluble vitamin c, glutathione and liposoluble vitamin E.Cause the condition of oxidative stress very much, disease, life
The variation of reason, environment and daily ration condition can all lead to oxidative stress.The present invention is selected from feedstuff, is immunized and anti-oxidant nutrition
Etc. start with, solve feed reason caused by oxidative stress, improve egg in vitamin E and Se accumulation amount, it is scientific and reasonable
ω -6 and ω -3 PUFA ratio, etc. integrated systems nutritional programs extend egg shelf life.
Hydrolysable Tannins acid, natural beta-carotin, natural carotenol have enhancing immune function and anti-oxidation function.
Palm-kernel oil is rich in medium chain fatty acid (C8:0—C14:0), have fabulous digestion and absorption and physiology special
Property, as a kind of effective energy, can fast direct switch through and be transported to liver and efficient oxidation function.With antibacterial and immunological regulation function
Energy.
The nutrition physiology of ω -6/ ω -3PUFA proportional balancing method acts on:1) ω -6PUFA can excessively cause blood viscosity, blood vessel
Spasm degree and vessel retraction degree increase, and increase ω -3 PUFA then and can be relieved these symptoms.2) ω -6/ ω -3 PUFA ratio is flat
Weighing apparatus influence gene expression regulation, 3) ω -6/ ω -3 PUFA proportional balancing method on lipid metabolism influence.ω -3 PUFA is to fatty acid
It is better than ω -6 PUFA with the inhibiting effect of fatty acid synthetase.The report such as Duplus, AA and its metabolin prostaglandin E
(PGE), especially prostaglandin E2 (PGE2) can react rapidly with fat cell transcription factor and activate it, and it is thin to activate fat
Born of the same parents promote its metabolism, so that body fat level be made to decline.ω -3 PUFA has reduction low density cholesterol and extra-low density
Cholesterol increases the effect of high-density lipoprotein, since high-density lipoprotein is a kind of cholesterol form for being conducive to health, it
Cholesterol can be made from being transferred in liver into the cell, to make cholesterol by liver metabolism.4) suitable ω -6/ ω -3
PUFA ratio is the key that research animal body immunity function.Because the two is catalyzed by the same enzyme is metabolized in vivo, machine is being influenced
Competitive effect occurs in terms of body immunologic mechanism.
Corn oil linoleic acid (ω -6) content is high, therefore ω -6:Omega-fatty acid ratio is just high, and linseed is rich in linolenic acid
(omega-fatty acid), then ω -6:Omega-fatty acid ratio is with regard to very low.ω -6/ ω -3 PUFA (polyunsaturated fatty acid) is most
Good ratio is reduced to as (2-4):When 1, egg oxidation deterioration degree is less than ω -6/ ω -3 PUFA and is greater than 4-6:1;This hair simultaneously
The bright vitamin E and synthesising complex E made full use of in raw material, and dosage foot, with yeast selenium in feed and suitable ω -6/
ω -3 PUFA (polyunsaturated fatty acid) combines antioxidation, overcomes the weakness of conventional egg feedstuff, reaches best antioxygen
Change horizontal.
Chili meal contains the substance there are many free radical resisting, has oxidation resistance, and vitamin C and Wei Sheng containing volume
Plain E;Tannic acid is a kind of polyphenol compound, is divided into Hydrolysable Tannins acid and condensed tannin acid, Hydrolysable Tannins acid (excellent can precious)
Effect:First is that having very strong astriction, intestines peristalsis can be reduced, slow down velocity of liquid assets of the chyme in enteron aisle, promote water
Divide the absorption with nutrition in substance;Second is that having the function of natural anti-oxidation, free radical is removed, there is protection to make biological tissue
With.Third is that antivirus action.Have the function of inhibiting the duplication of the Respirovirus such as influenza, is able to suppress the conjunction of viral glycoprotein
At to achieve the effect that antiviral.Fourth is that selectivity is antibacterial, the growth and breeding of the beneficial bacteriums such as lactic acid bacteria is improved, inhibits leather blue
The breeding of family name's negative bacterium and other pathogens effectively solves the problems, such as laying hen diarrhea.
There is apparent sour taste using the biological fermentation feed of the compound preparation of multi-cultur es, the cream in probiotics metabolite
Acid, bacteriocin etc. have common pathogen such as Escherichia coli, salmonella, C.perfringens, staphylococcus aureus bright
Aobvious inhibitory effect can be improved laying hen to the resistance of bacteriosis, reduce the poisonous and harmful substance that pathogenic bacteria generate with
And the production of oxyradical, bacillus coagulans and saccharomycete generate a large amount of protease during the growth process, it will be in substrate
Macro-molecular protein is decomposed into polypeptide, small peptide and amino acid, improves the digestion and absorption efficiency of substrate nutriment, and reduces antigen
The content of albumen and trypsin ihhibitor, saccharomycete generates a large amount of flavor substances, improves the palatability of fermented feed, and plants
Object lactobacillus, Pediococcus acidilactici then consume the bad oligosaccharides in substrate, and generation is conducive to the metabolism such as lactic acid of animal intestinal tract health
Product;A large amount of B family vitamins are also generated in these four microbial fermentation solutions and solid fermentation process, greatly improve fermentation
The nutritive value of feed, so that laying hen is after fermented feed of searching for food, enteron aisle is more healthy, to disease and stress resistance it is obvious
The digestion and absorption efficiency of enhancing, nutriment improves, to reach improvement eggshell strength, increases Vitamin E levels, extends shelf
The effect of phase.Biological feedstuff prepared by the present invention can improve laying hen intestinal health, and intestinal epithelial cell densification is promoted to connect egg
White expression stimulates the secretion of the glycoprotein of sIgA, improves laying hen intestinal immunity.
By above-mentioned raw materials, science compounds the present invention according to a certain percentage, each raw material synergistic effect, synergy each other, egg production
Within 48 hours time, detected value Hough unit reaches 90 or more, and albumen concentration is high, just can guarantee that the resting period is long, reaches and prolongs
The purpose of long shelf life.Under refrigerated conditions, egg of the present invention 80 days when Hough unit still reach 83.84, be higher than AA grades it is fresh
Egg is horizontal, and yolk index and gas chamber height marker are good, and mass loss rate extremely low 80 days only 3.87%.4-8 DEG C of the present invention
Shelf life can reach 80 days under refrigerated condition, extend one times on year-on-year basis, have very high market application value.Present invention feeding
Under the egg room temperature of production at 20-25 DEG C placement 21 days, Hough unit ratio market egg mean height 8.05%, yolk index is average
High by 11.04%, mass loss rate is significantly lower than market egg;When placing 50 days, egg yolk and egg white are all very good, Hough list
Position average out to 76.08 reaches fresh hen egg level;The egg for feeding market material, the scattered clear scattered Huang of egg, this hair when placing 40 days
Bright egg yolk and egg white are all very good, and mass loss rate egg of the present invention has a clear superiority, and are averagely lower than market egg
13.0%, air room height is averagely lower than market egg 11.74%, shows excellent effect.Conclusion, egg of the present invention under room temperature
Than the shelf life that market egg is able to extend 10 days or more.
The present invention is theoretical using system trophism, comprehensive from intestinal health, immune function, anti-oxidation function, raising premunition etc.
Conjunction factor is set about, and is significantly improved egg Hough unit, albumen height, eggshell strength, shell thickness, is effectively extended the goods of egg
The frame phase.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Range, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
Under the premise of invention spirit and scope, to the various changes or change of material component and dosage progress in these embodiments
It belongs to the scope of protection of the present invention.
Embodiment 1
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
20 parts of corn, 30 parts of barley, 8 parts of dregs of beans, 2 parts of expanded soybean, 5 parts of extruding linseed, 5.8 parts of corn protein powder,
It is 3.2 parts of sesame seed meal, 5 parts of rice bran, 2.5 parts of hot chili residue, 0.5 part of palm-kernel oil, 3.3 parts of malt root, 2 parts of biological fermentation feed, excellent
Can be 0.05 part precious, 0.025 part of yeast selenium, 0.02 part of vitamin E, 0.02 part of beta carotene, 0.1 part of natural carotenol, amino acid
0.67 part, 0.01 part of zinc albuminate, 0.012 part of albumen manganese, 10,000 0.02 part of unit phytases, 0.03 part of choline chloride, glycine betaine
0.08 part, 0.05 part of complex enzyme formulation, 10 parts of mountain flour, 0.8 part of calcium monohydrogen phosphate, 0.3 part of salt, 0.5 part of compound premix.
The laying cycle of laying hens feed, it is 3.5 that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,:1;
The amino acid is 0.35 part of lysine, and DL- METHIONINE is 0.175 part, and threonine is 0.105 part, and tryptophan is
0.0385 part;Lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Threonine:Tryptophan=1:
0.5:0.3:0.11 uniformly mixing.
The compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, product approval
Code is that pre- word (2013) 003024 is raised by the Liao Dynasty;
The 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence number are to raise to add
(2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;
The complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise to add (2011)
1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;
It is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;
The biological fermentation feed, main active be bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici,
Saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are greater than
Equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:3:6 mass ratio and beans
The dregs of rice, rice bran meal mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, on
The mixing quality ratio for stating four kinds of bacterium solutions is 1:2:2:3, the mass ratio of bacterium solution total amount and fermentation substrate is 1:3;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 5 days, and temperature is 35 DEG C, and material pH value drops to 5.0, and bacillus coagulans, lactic acid bacteria and yeast bacterial content difference are big
In equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
The preservation of bacteria strain is purchased from China General Microbiological culture presevation administrative center, and strain properties are as follows:
Bacillus coagulans CGMCC No.1.3220:Gram-positive, rod-short produce gemma, in tryptone agar
For white protrusion bacterium colony, 30-37 degrees Celsius of well-grown, degradable macro-molecular protein generates small peptide active material;
Lactobacillus plantarum CGMCC No.1.3:Gram-positive, it is rod-shaped, gemma is not produced, on lactic acid bacteria agar culture medium
For milky protrusion bacterium colony, 30-37 degrees Celsius of well-grown generates the metabolites such as lactic acid using oligosaccharides;
Pediococcus acidilactici CGMCC No.1.2696:Gram-positive, it is spherical, gemma is not produced, in lactic acid bacteria agar culture medium
Upper is milky protrusion bacterium colony, and 30-37 degrees Celsius of well-grown generates the metabolites such as lactic acid using oligosaccharides;
Saccharomyces cerevisiae CGMCC No.2.1792:Fungi is white circular edge on potato dextrose agar
Smooth protrusion bacterium colony, 25-30 degrees Celsius of well-grown generate the metabolites such as alcohol, solid culture using glucose
With apparent fragranced.
The preparation method of the laying cycle of laying hens feed for extending egg shelf life:Each component original is accurately weighed according to formula
Material is crushed according to conventional egg feedstuff processing method, then arrogant to small in parts by weight to sequentially add various raw materials, mixing
Uniformly, it processes to get a kind of laying cycle of laying hens feed for extending egg shelf life.
Embodiment 2
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
30 parts of corn, 23 parts of barley, 5 parts of dregs of beans, 5 parts of expanded soybean, 8 parts of extruding linseed, 3 parts of corn protein powder, sesame
2 parts of the numb dregs of rice, 3 parts of rice bran, 1 part of hot chili residue, 0.5 part of palm-kernel oil, 5 parts of malt root, 0.8 part of biological fermentation feed, it is excellent can be precious
0.03 part, 0.025 part of yeast selenium, 0.01 part of vitamin E, 0.04 part of beta carotene, 0.08 part of natural carotenol, amino acid
0.45 part, 0.015 part of zinc albuminate, 0.015 part of albumen manganese, 10,000 0.01 part of unit phytases, 0.04 part of choline chloride, glycine betaine
0.06 part, 0.1 part of complex enzyme formulation, 9 parts of mountain flour, 0.8 part of calcium monohydrogen phosphate, 0.4 part of salt, 0.5 part of compound premix.
The laying cycle of laying hens feed, it is 3.2 that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,:1.
The amino acid is 0.19 part of lysine, and DL- METHIONINE is 0.114 part, and threonine is 0.116 part, and tryptophan is
0.0285 part;Lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Threonine:Tryptophan=1:
0.6:0.61:0.15 uniformly mixing.
The compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, product approval
Code is that pre- word (2013) 003024 is raised by the Liao Dynasty;
The 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence number are to raise to add
(2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;
The complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise to add (2011)
1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;
It is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;
The biological fermentation feed, main active be bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici,
Saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are greater than
Equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:2:7 mass ratio and beans
The dregs of rice, rice bran meal mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, on
The mixing quality ratio for stating four kinds of bacterium solutions is 1:2:2:1, the mass ratio of bacterium solution total amount and fermentation substrate is 1:4;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 3 days, and temperature is 30 DEG C, and material pH value drops to 5.5, and bacillus coagulans, lactic acid bacteria and yeast bacterial content difference are big
In equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
A kind of preparation method for the laying cycle of laying hens feed extending egg shelf life, with embodiment 1.
Embodiment 3
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
35 parts of corn, 15 parts of barley, 6 parts of dregs of beans, 3.5 parts of expanded soybean, 3 parts of extruding linseed, 8 parts of corn protein powder,
4 parts of sesame seed meal, 8 parts of rice bran, 3 parts of hot chili residue, 1.0 parts of palm-kernel oil, 1 part of malt root, 2 parts of biological fermentation feed, it is excellent can be precious
0.05 part, 0.025 part of yeast selenium, 0.02 part of vitamin E, 0.02 part of beta carotene, 0.05 part of natural carotenol, amino acid
0.59 part, 0.01 part of zinc albuminate, 0.015 part of albumen manganese, 10,000 0.02 part of unit phytases, 0.05 part of choline chloride, glycine betaine
0.03 part, 0.1 part of complex enzyme formulation, 8 parts of mountain flour, 0.5 part of calcium monohydrogen phosphate, 0.35 part of salt, 0.5 part of compound premix.
The laying cycle of laying hens feed, it is 4.0 that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,:1.
The amino acid is 0.27 part of lysine, and DL- METHIONINE is 0.148 part, and threonine is 0.138 part, and tryptophan is
0.0351 part;Lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Threonine:Tryptophan=1:
0.55:0.51:0.13 uniformly mixing.
The compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, product approval
Code is that pre- word (2013) 003024 is raised by the Liao Dynasty;
The 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence number are to raise to add
(2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;
The complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise to add (2011)
1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;
It is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;
The biological fermentation feed, main active be bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici,
Saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are greater than
Equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:4:5 mass ratio and beans
The dregs of rice, rice bran meal mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, on
The mixing quality ratio for stating four kinds of bacterium solutions is 1:1:3:4, the mass ratio of bacterium solution total amount and fermentation substrate is 1:2;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 7 days, and temperature is 40 DEG C, and material pH value drops to 4.0, and bacillus coagulans, lactic acid bacteria and yeast bacterial content difference are big
In equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
A kind of preparation method for the laying cycle of laying hens feed extending egg shelf life, with embodiment 1.
Embodiment 4
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
25 parts of corn, 28 parts of barley, 7 parts of dregs of beans, 4 parts of expanded soybean, 5 parts of extruding linseed, 6.2 parts of corn protein powder,
3.1 parts of sesame seed meal, 7.3 parts of rice bran, 2.6 parts of hot chili residue, 0.8 part of palm-kernel oil, 2.2 parts of malt root, 2 parts of biological fermentation feed,
It is excellent can be 0.05 part precious, 0.025 part of yeast selenium, 0.02 part of vitamin E, 0.02 part of beta carotene, 0.05 part of natural carotenol, ammonia
0.64 part of base acid, 0.01 part of zinc albuminate, 0.015 part of albumen manganese, 10,000 0.02 part of unit phytases, 0.05 part of choline chloride, beet
0.03 part of alkali, 0.1 part of complex enzyme formulation, 8.5 parts of mountain flour, 0.6 part of calcium monohydrogen phosphate, 0.35 part of salt, 0.5 part of compound premix.
The laying cycle of laying hens feed, it is 3.6 that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,:1.
The amino acid is 0.3 part of lysine, and DL- METHIONINE is 0.153 part, and threonine is 0.15 part, and tryptophan is
0.039 part;Lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Threonine:Tryptophan=1:
0.51:0.5:0.13 uniformly mixing.
The compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, product approval
Code is that pre- word (2013) 003024 is raised by the Liao Dynasty;
The 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence number are to raise to add
(2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;
The complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise to add (2011)
1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;
It is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;
The biological fermentation feed, main active be bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici,
Saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are greater than
Equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:2:5 mass ratio and beans
The dregs of rice, rice bran meal mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, on
The mixing quality ratio for stating four kinds of bacterium solutions is 1:2:3:5, the mass ratio of bacterium solution total amount and fermentation substrate is 1:3;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 6 days, and temperature is 33 DEG C, and material pH value drops to 4.8, and bacillus coagulans, lactic acid bacteria and yeast bacterial content difference are big
In equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
A kind of preparation method for the laying cycle of laying hens feed extending egg shelf life, with embodiment 1.
Embodiment 5
A kind of laying cycle of laying hens feed extending egg shelf life is prepared by the raw material of following parts by weight:
30 parts of corn, 23 parts of barley, 6 parts of dregs of beans, 3 parts of expanded soybean, 5 parts of extruding linseed, 5 parts of corn protein powder, sesame
2.5 parts of the numb dregs of rice, it is 6.9 parts of rice bran, 2.3 parts of hot chili residue, 0.6 part of palm-kernel oil, 2.0 parts of malt root, 2 parts of biological fermentation feed, excellent
Can be 0.03 part precious, 0.025 part of yeast selenium, 0.02 part of vitamin E, 0.04 part of beta carotene, 0.1 part of natural carotenol, amino acid
0.49 part, 0.01 part of zinc albuminate, 0.012 part of albumen manganese, 10,000 0.02 part of unit phytases, 0.05 part of choline chloride, glycine betaine
0.06 part, 0.1 part of complex enzyme formulation, 8.5 parts of mountain flour, 0.8 part of calcium monohydrogen phosphate, 0.3 part of salt, 0.5 part of compound premix.
The laying cycle of laying hens feed, it is 3.8 that nutritive index, which is set as ω -6/ ω -3 PUFA ratio,:1.
Further, amino acid is 0.25 part of lysine, and DL- METHIONINE is 0.128 part, and threonine is 0.075 part, color ammonia
Acid is 0.0375 part;Lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Threonine:Tryptophan
=1:0.51:0.3:0.15 uniformly mixing.
The amino acid be lysine, methionine, threonine and tryptophan in mass ratio, lysine:Methionine:Soviet Union's ammonia
Acid:Tryptophan=1:0.5-0.6:0.3-0.61:0.11-0.15 is uniformly mixed.
The compound premix:Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, product approval
Code is that pre- word (2013) 003024 is raised by the Liao Dynasty;
The 10000 unit phytase:Beijing Smistyle Science & Technology Development Co., Ltd.'s production, production licence number are to raise to add
(2012) 0017, authentication code:Raise (adding) word (2012) 403711 in capital;
The complex enzyme formulation:Shenyang Fengmei Biotechnology Co., Ltd.'s production, production licence number are to raise to add (2011)
1986, authentication code:(adding) word (2011) 040008 is raised by the Liao Dynasty;
It is described it is excellent can be precious:Quasi- word 328 is raised in Italian Shi Hua company, (2011) outside;
The biological fermentation feed, main active be bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici,
Saccharomyces cerevisiae and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are greater than
Equal to 20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has apparent sour taste.
The biological fermentation feed preparation method is as follows:
1. by preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, plant cream
Bacillus CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar (MRS), saccharomyces cerevisiae
CGMCC No.2.1792 streak inoculation cultivates 48 hours in 30 DEG C of potato dextrose agar (PDA) to growing apparent single bacterium
It falls;
2. the picking single colonie on the culture medium of four kinds of bacterium respectively, be correspondingly inoculated in 2L liquid tryptone, MRS,
On MRS, Potato-dextrose meat soup (PDB) culture medium, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
3. cultured liquid seeds liquid is inoculated in 500L liquid tryptone respectively, in MRS, MRS, PDB culture medium,
30 DEG C, 200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
4. corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:4:7 mass ratio and beans
The dregs of rice, rice bran meal mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, on
The mixing quality ratio for stating four kinds of bacterium solutions is 1:1:1:1, the mass ratio of bacterium solution total amount and fermentation substrate is 1:2;
5. uniformly mixed bacterium solution and fermentation materials are placed in constant-temperature fermentation tank to stack, 4 meters of groove width, piling height 1-1.5
Rice ferments 4 days, and temperature is 38 DEG C, and material pH value drops to 5.2, and bacillus coagulans, lactic acid bacteria and yeast bacterial content difference are big
In equal to 108CFU/g, which ferments, to be completed.
It is raised 6. the material of fermentation ends is placed in fluidized bed to dry to moisture content 10% hereinafter, obtaining biofermentation
Material.
A kind of preparation method for the laying cycle of laying hens feed extending egg shelf life, with embodiment 1.
Test example extends the laying cycle of laying hens feed of egg shelf life with the present invention and commercially available similar feed does control examination
It tests, influence of more each feed to laying hen antioxygenic property, egg laying performance and egg shelf life.Comprehensive 23 campaigns, allusion quotation
Type Test Summary is as follows:
1 experiment arranges
1.1 2017 on May 20, -- July 23, test chicken 266 (38W) -- 323 ages in days (46W), pre-feeding period 7 days, examination
Test the phase 58 days.
1.2 personnel:Wang Yu Lin (experimental design is organized and coordinated, field observation, laboratory report summary), (physiology is raw by Liu Minyue
Change index determining), Zhang Xin (live Indexs measure, data statistic analysis), Li Dechen (field experiment person and live index determining).
2. experimental design and material:
2.1 2 groups (feed of the present invention and market feed) of tests point:Using sea blue brown laying hen, every group of 6 repetitions, Mei Gechong
Multiple 288 chickens, amount to 3456 chickens.
2.2 material:
Feed of the present invention, market sale feed
2.2.1 Feed Manufacturing:The rich production of standing grain in Fuyu County
2.2.2 test chicken needs to choose chicken:Weight is excessive, and underweight, diseased chicken is all chosen.
2.3 material-changing method:Transition 9 days, first 3 days conventional material 2/3+1/3 testing materials, 3 days conventional material 1/2+1/2 testing materials, 3
It routinely expects 1/3+2/3 testing material.
2.4 detection record indexes:
1) it weighs:Original body mass terminates weight.The weighing of 4-6 point in afternoon.Weight is each to be repeated to weigh 4 chickens, and whole cage is by only
It weighs, performs mark.
2) feed intake:Unified scale of feeding is 120 grams;
3) egg laying performance:Record chicken number, piece number of laying eggs, total egg size, broken egg number situation daily, examine egg arrangement of time under
Noon 10:30---11:30 points
4) temperature (8 points of 2 pm and evening), humidity;When daily temperature and special weather situation;
5) egg product quality detection:Each repetition takes 30 pieces of eggs at random, is detected within 7 days before off-test.Testing index:It breathes out
The indexs such as husband's unit, air room height, yolk index, eggshell strength, shell thickness.Egg product quality detection produces multi-functional egg using Japan
Product analyzer EMT-7300, eggshell strength are laid eggs shell intensity meter FESTV2.0 using Germany, and shell thickness is laid eggs shell using Japan
Thickness gauge ETG-1061.
6) indexs such as content of fatty acid, Se content, content in 7 days detection eggs before off-test.
3 test results
Influence of 3.1 feeds of the present invention to laying hen in egg laying peak period egg laying performance, the results are shown in Table 1
Influence of the feed of the present invention of table 1 to performance in layers
Market material | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
Chicken number (only) | 1728 | 1728 | 1728 | 1728 | 1728 | 1728 |
Laying rate (%) | 93.23±1.78a | 97.92±1.66b | 98.03±1.32b | 94.36±1.57b | 95.09±1.44b | 95.57±1.23b |
Egg size (g) | 60.71±0.74 | 60.90±0.69 | 61.82±0.37 | 62.03±0.57 | 61.26±0.61 | 61.85±0.69 |
Egg production (gram/only/day) | 56.60±1.26 | 59.63±1.12 | 60.6 | 58.53 | 58.25 | 59.11 |
Feed intake (g/d) | 120 | 120 | 120 | 120 | 120 | 120 |
Feedstuff-egg ratio (%) | 2.12:1 | 2.01:1 | 1.98:1 | 2.05:1 | 2.06:1 | 2.03:1 |
Same column difference lowercase letter indication difference is significant (P < 0.05), and same column difference capitalization indicates that difference is extremely significant
(P < 0.01), following table is same.
Feed of the present invention is in the identical situation of feed intake, and laying rate improves 1.86% or more than market material, egg production
Improve 1.65 grams/only/it is more than day, egg size difference seldom, it is minimum only 0.19 gram/piece.Feed egg laying performance of the present invention is every
Index is apparently higher than market material.
Influence of 3.2 feeds of the present invention to Egg Quality, the results are shown in Table 2
Table 2:Testing result within egg of the present invention is placed 24 hours
The present invention experiments have shown that, fresh hen egg Hough unit be respectively higher than market expect 6.19%, 8.33,2.73,6.45% and
3.97%, egg Hough unit of the present invention is all higher than 90.68 or more, and albumen height is all higher than 9.05 or more;Air room height
At least below market material 17.58%, the Egg Qualities index such as yolk color, albumen height is superior to market material;Shell thickness, eggshell
The index of the quality of egg such as intensity and eggshell ratio is also significantly better than market material.It is dense to will become apparent from albumen concentration, yolk height is high,
Vitellinae membrana is solid, and Hough unit is high, and all these is all the qualitative characteristics of excellent egg.
Influence of 3.3 feeds of the present invention to fatty acid in egg
The testing result of fatty acid and selenium in the egg of the present invention of table 3
ω -6 in egg of the present invention:ω -3 value is far below market material group, and omega-fatty acid summation reaches 410.55-
430.32mg/100g egg is higher than market and expects one times or more.According to the standard of European Union, every piece of egg can provide adult daily requirement
The 15% of amount (55ug) is properly termed as selenium source (source of selenium), and 30% is properly termed as high selenium (high
selenium).According to this standard, the Se content standard of " selenium source " and " high selenium " egg is respectively every piece of egg of 8.25 and 16.5ug/.
Se content is all higher than every piece of egg of 22.85ug/ in egg of the present invention, belongs to Se-enriched egg.
Influence of 3.4 present invention to egg shelf life
Air room height:Egg is placed on a dark place, with flashlight against the blunt nosed photograph of egg, is drawn with marking pen
The profile of gas chamber, syringe needle stave eggshell, with the depth of vernier caliper measurement gas chamber, as air room height.The lower table of air room height
Freshness is good for bright egg.
Hangh unit is that Haugh is proposed in nineteen thirty-seven.It is according to egg size and dense albumen height, by formula HU=
(wherein HU is Kazakhstan value to the index of the calculated evaluation egg quality of 100Lg (H+7.57-1.7w0.37), and H is dense albumen
Highly, W is egg size), it is the main indicator for evaluating egg quality, United States Department of Agriculture's egg product standard test and expression egg product freshness
Index.According to the standard, for hangh unit 72 the above are AA grades, 71-55 is A grade, and 54-31 is B grades, and 30 the following are C grades (A grades
And the above are table egg, B grades are preserved egg, and C grades only partially for processing).
Yolk coefficient:Egg content is all poured into horizontal positioned plate, most with vernier caliper measurement vertical direction
Big thickness is yolk height, simultaneously yolk diameter (being had a common boundary with yolk and egg white for line), yolk height and yolk diameter ratio
For yolk coefficient.Take the average value of 30 pieces of eggs.The higher egg freshness of yolk coefficient is better.
Mass loss rate:30 pieces of egg quality are detected with electronic balance, are averaged.Matter after mass loss rate=placement
Quality * 100 before amount/placement.Mass loss rate is lower, and egg freshness is better.
Table 4:Egg shelf life Testing index under the conditions of 4-7 DEG C of refrigeration
As seen from the above table, under refrigerated condition, cold storage of eggs mass loss rate, air room height, the ovum of feed of the present invention are fed
Yellow coefficient, Hough unit indices are significantly better than that the egg of the feeding similar feed in market, and as time went on, difference is got over
Hair is significant.Dissipate within market material group egg 60 days it is clear, dissipate within 80 days it is clear, dissipate Huang, and Hough unit still reaches at of the present invention group egg 80 days
To 83.84 or more, it is higher than AA grades of fresh hen eggs levels, yolk index and gas chamber height marker are good, and mass loss rate is extremely low, and 80 days
Only 3.87%.Shelf life can reach 80 days when 4-8 DEG C of refrigeration of egg of the present invention.
Table 5:By the egg of egg of the present invention and feeding market material, one is stored at 20-25 DEG C of room temperature under the conditions of same
It is detected after fixing time, it is as a result as follows:
As seen from the above table, when the present invention feeds 20-25 DEG C placement 21 days under the egg room temperature produced, Hough unit ratio market
Egg mean height 8.05%, yolk index mean height 11.04%, mass loss rate are significantly lower than market egg;When placing 50 days,
Egg yolk and egg white are all very good, Hough unit average out to 76.08, reach fresh hen egg level;The egg for feeding market material, puts
Egg scattered clear scattered Huang when setting 40 days, egg yolk and egg white of the present invention are all very good, mass loss rate egg tool of the present invention
It has a clear superiority, is averagely lower than market egg 13.0%, air room height is averagely lower than market egg 11.74%, shows excellent
Effect.Conclusion, egg of the present invention is able to extend 10 days or more shelf life than market egg under room temperature.
4. conclusion
Egg shelf life is long, illustrates high-quality egg quality, safety, nutrition, and antioxidants content is high in egg.It grinds
The essence for studying carefully nutrition and shelf life is exactly the relationship studied between nutrition and Quality eggs, previous data seldom nutritious and shelf
Research and report in terms of phase.
Conclusion (of pressure testing) thinks, selenium, vitamin E, beta carotene, omega-fatty acid summation, ω -6 in feed nutrition:ω-
The nutrients such as 3PUFA ratio bioaccumulation efficiency in egg is high, and using the egg feedstuff of ideal amino acid ratio science compounding
Egg Quality and egg shelf life are had a significant impact, 4-8 DEG C of egg shelf life of refrigeration can extend to 80 days;20-25 DEG C of room temperature
Shelf life extension was by 50 days.The present invention extends the feed of egg shelf life, is conducive to the production of food safety and high-quality egg product, has
It increases economic efficiency conducive to egg circulation.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
The limitation to the scope of the patents therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Under the premise of not departing from present inventive concept, the respective embodiments described above can also make combination and improvement, can also other birds or dynamic
Object cultivation, to extend the shelf life of egg product, these are all within the scope of protection of the present invention.
Claims (10)
1. a kind of preparation method of biological fermentation feed, includes the following steps:
By preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, lactobacillus plantarum
CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are in lactic acid bacteria agar, saccharomyces cerevisiae CGMCC
No.2.1792 streak inoculation is in potato dextrose agar, and 30 DEG C of cultures 48 hours are to growing single colonie;
The picking single colonie on the culture medium of four kinds of bacterium respectively is inoculated in 2L liquid tryptone, MRS, MRS, potato grape
On carbohydrate broth culture medium, 30 DEG C, 200RPM shaking table culture 48 hours;
Cultured liquid seeds liquid is inoculated in 500L liquid tryptone, in MRS, MRS, PDB culture medium respectively, 30 DEG C,
200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
Crush maize crosses 3mm sieve, according to 1:2-4:The mass ratio of 5-7 is mixed with dregs of beans, rice bran meal, and four kinds of probiotics are added
Bacterium solution, the mixing quality ratio of above-mentioned four kinds of bacterium solutions are 1:1-2:1-3:1-5 is uniformly mixed;Bacterium solution total amount and fermentation substrate
Mass ratio is 1:2-4;
30-40 DEG C ferment at constant temperature 3-7 days, material pH value drops to 4.0-5.5, and bacillus coagulans, lactic acid bacteria and saccharomycete contain
Amount, which is respectively greater than, is equal to 108CFU/g, which ferments, to be completed.
2. a kind of laying cycle of laying hens feed for extending egg shelf life is prepared by the raw material of following parts by weight:
20-35 parts of corn, 15-30 parts of barley, 5-8 parts of dregs of beans, 2-5 parts of expanded soybean, 3-8 parts of extruding linseed, zein
3-8 parts of powder, 2-4 parts of sesame seed meal, 3-8 parts of rice bran, 1-3 parts of hot chili residue, 0.5-1.0 parts of palm-kernel oil, 1-5 parts of malt root, biology
0.8-2 parts of fermented feed, it is excellent can 0.03-0.05 parts of treasured, 0.025 part of yeast selenium, 0.01-0.02 parts of vitamin E, beta carotene
0.02-0.04 parts, 0.05-0.1 parts of natural carotenol, 0.45-0.67 parts of amino acid, 0.01-0.015 parts of zinc albuminate, albumen manganese
0.01-0.015 parts, 10,000 0.01-0.02 parts of unit phytases, 0.03-0.05 parts of choline chloride, 0.03-0.08 parts of glycine betaine,
0.05-0.1 parts of complex enzyme formulation, 8-10 parts of mountain flour, 0.5-0.8 parts of calcium monohydrogen phosphate, 0.3-0.4 parts of salt, compound premix 0.5
Part, which is characterized in that the biological fermentation feed is prepared by the method as described in claim 1.
3. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that the laying hen produces
Egg phase feed, it is 3.2-4.0 that nutritive index, which is set as ω -6/ ω -3PUFA ratio,:1.
4. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that the amino acid
For in mass ratio, lysine:Methionine:Threonine:Tryptophan=1:0.5-0.6:0.3-0.61:0.11-0.15 is uniformly mixed.
5. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that described compound pre-
Mixing composition is as follows:Iron 15000mg/Kg, copper 3000mg/Kg, zinc 20000mg/Kg, manganese 24000mg/Kg, selenium 60mg/Kg, iodine
100mg/Kg, vitamin A 1600KIU, vitamin D 600KIU, vitamin E 320mg/Kg, vitamin K3200mg/Kg, dimension
Raw element B1200mg/Kg, vitamin B2880mg/Kg, vitamin B6600mg/Kg, vitamin B122.0mg/Kg, niacin
6000mg/Kg, pantothenic acid 800mg/Kg, folic acid 100.0mg/Kg, biotin 5.5mg/Kg.
6. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that the complex enzyme
Preparation Contents of Main Components is as follows:Zytase 3000U/g, mannase 800U/g, acid protease 2000U/g, fat
Enzyme 800U/g, amylase 300U/g, dextranase 400U/g.
7. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that by following weight
It is prepared by the raw material of number:
20 parts of corn, 30 parts of barley, 8 parts of dregs of beans, 2 parts of expanded soybean, 5 parts of extruding linseed, 5.8 parts of corn protein powder, sesame
3.2 parts of the dregs of rice, 5 parts of rice bran, 2.5 parts of hot chili residue, 0.5 part of palm-kernel oil, 3.3 parts of malt root, 2 parts of biological fermentation feed, it is excellent can be precious
0.05 part, 0.025 part of yeast selenium, 0.02 part of vitamin E, 0.02 part of beta carotene, 0.1 part of natural carotenol, amino acid 0.67
Part, 0.01 part of zinc albuminate, 0.012 part of albumen manganese, 10,000 0.02 part of unit phytases, 0.03 part of choline chloride, glycine betaine 0.08
Part, 0.05 part of complex enzyme formulation, 10 parts of mountain flour, 0.8 part of calcium monohydrogen phosphate, 0.3 part of salt, 0.5 part of compound premix;
The laying cycle of laying hens feed, it is 3.5 that nutritive index, which is set as ω -6/ ω -3PUFA ratio,:1;
The amino acid is 0.35 part of lysine, and 0.175 part of DL- METHIONINE, 0.105 part of threonine, 0.0385 part of tryptophan is
Even mixing;
The biological fermentation feed, main active are bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici, wine brewing ferment
Female and its metabolite, wherein bacillus coagulans content is more than or equal to 108CFU/g, finished product crude protein are more than or equal to 20%, water
Divide less than 10%, pH value 4.0-5.5, total acid content 2-15% have sour taste;
The biological fermentation feed preparation method is as follows:
By preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220, lactobacillus plantarum CGMCC No.1.3, Pediococcus acidilactici
CGMCC No.1.2696, saccharomyces cerevisiae CGMCC No.2.1792 distinguish streak inoculation in tryptone agar, MRS, MRS, PDA
Agar, 30 DEG C culture 48 hours to growing single colonie;
The picking single colonie on the culture medium of four kinds of bacterium respectively is inoculated in 2L liquid tryptone, MRS, MRS, PDB culture medium,
30 DEG C in shaking table, 200RPM is cultivated 48 hours;
Cultured liquid seeds liquid is inoculated in 500L liquid tryptone, in MRS, MRS, PDB culture medium respectively, 30 DEG C,
200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
Corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:3:6 ratio and dregs of beans, rice bran meal are mixed
It closes, and above-mentioned bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae bacterium solution is added, above-mentioned four kinds of bacterium solutions
Mixing quality ratio is 1:2:2:3, it is uniformly mixed, the ratio of bacterium solution total amount and fermentation substrate is 1:3;
Uniformly mixed bacterium solution is fermented 5 days with 35 DEG C of fermentation materials, material pH value drops to 5.0, bacillus coagulans, lactic acid
Bacterium and yeast bacterial content, which are respectively greater than, is equal to 108CFU/g, which ferments, to be completed;
By the dry materials of fermentation ends to moisture content 10% hereinafter, obtaining biological fermentation feed finished product.
8. the laying cycle of laying hens feed according to claim 2 for extending egg shelf life, which is characterized in that by following weight
It is prepared by the raw material of number:
30 parts of corn, 23 parts of barley, 5 parts of dregs of beans, 5 parts of expanded soybean, 8 parts of extruding linseed, 3 parts of corn protein powder, sesame seed meal 2
Part, 3 parts of rice bran, 1 part of hot chili residue, 0.5 part of palm-kernel oil, 5 parts of malt root, 0.8 part of biological fermentation feed, it is excellent can be 0.03 part precious,
0.025 part of yeast selenium, 0.01 part of vitamin E, 0.04 part of beta carotene, 0.08 part of natural carotenol, 0.45 part of amino acid, egg
0.015 part of white zine, 0.015 part of albumen manganese, 10,000 0.01 part of unit phytases are 0.04 part of choline chloride, 0.06 part of glycine betaine, multiple
0.1 part of synthase preparation, 9 parts of mountain flour, 0.8 part of calcium monohydrogen phosphate, 0.4 part of salt, 0.5 part of compound premix;
The laying cycle of laying hens feed, it is 3.2 that nutritive index, which is set as ω -6/ ω -3PUFA ratio,:1;
The amino acid is 0.19 part of lysine, and 0.114 part of DL- METHIONINE, 0.116 part of threonine, 0.0285 part of tryptophan is
Even mixing;
The biological fermentation feed, main active are bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici, wine brewing ferment
Female and its metabolite, wherein coagulated bacillus living content is more than or equal to 108CFU/g, finished product crude protein are more than or equal to
20%, moisture is less than 10%, pH value 4.0-5.5, total acid content 2-15%, has sour taste;
The biological fermentation feed preparation method is as follows:
By preservation of bacteria strain Bacillus coagulans CGMCC No.1.3220 streak inoculation in tryptone agar, lactobacillus plantarum
CGMCC No.1.3, Pediococcus acidilactici CGMCC No.1.2696 streak inoculation are drawn in MRS, saccharomyces cerevisiae CGMCC No.2.1792
Line is inoculated in PDA, and 30 DEG C of cultures 48 hours are to growing single colonie;
The picking single colonie on the culture medium of four kinds of bacterium respectively is correspondingly inoculated in 2L liquid tryptone, MRS, MRS, PDB training
It supports on base, 30 DEG C in shaking table, 200RPM is cultivated 48 hours;
Cultured liquid seeds liquid is inoculated in 500L liquid tryptone, in MRS, MRS, PDB culture medium respectively, 30 DEG C,
200RPM is cultivated 24 hours, obtains four kinds of probiotics bacterial liquids;
Corn beater disintegrating machine is crushed and is sieved, sieve aperture 3mm, according to 1:2:7 mass ratio and dregs of beans, rice bran
Dregs of rice mixing, and the mixed bacteria liquid of bacillus coagulans, lactobacillus plantarum, Pediococcus acidilactici and saccharomyces cerevisiae is added, above-mentioned four kinds
The mixing quality ratio of bacterium solution is 1:2:2:1, the mass ratio of bacterium solution total amount and fermentation substrate is 1:4;
Uniformly mixed bacterium solution is fermented 3 days with 30 DEG C of fermentation materials, material pH value drops to 5.5, bacillus coagulans, lactic acid
Bacterium and yeast bacterial content, which are respectively greater than, is equal to 108CFU/g, which ferments, to be completed;
By the material of fermentation ends be placed in fluidized bed dry to moisture content 10% hereinafter, obtain biological fermentation feed at
Product.
9. the preparation method of any laying cycle of laying hens feed for extending egg shelf life of claim 2-8, including following step
Suddenly:
Each component raw material is accurately weighed according to formula, is crushed according to conventional egg feedstuff processing method, then in parts by weight
It is arrogant to small to sequentially add various raw materials, it is uniformly mixed, processes and raised to get a kind of laying cycle of laying hens for extending egg shelf life
Material.
10. a kind of laying high selenium eggs of long shelf life, the egg selenium concentration is higher than every piece of egg of 22.85ug/, and 4-8 DEG C of shelf life reaches
By 80 days, 20-25 DEG C of shelf life reached 50 days, which is characterized in that the production method of the egg includes:In laying cycle of laying hens,
Feed any laying cycle of laying hens feed for extending egg shelf life of claim 2-8.
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CN110463824A (en) * | 2019-08-19 | 2019-11-19 | 四川农业大学 | Ovshinsky lactobacillus strain BSLO 1801 and its application in Egg Production of Laying Hens |
CN112369531A (en) * | 2020-12-03 | 2021-02-19 | 湖南省畜牧兽医研究所 | Feed additive for reducing fresh egg yolk-dispersing rate |
CN115316548A (en) * | 2022-08-24 | 2022-11-11 | 英联饲料(辽宁)有限公司 | Special chicken feed capable of improving nutrient utilization rate by using complex enzyme preparation |
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