CN108849986A - 西姆芽孢杆菌在防治白腐病中的应用 - Google Patents

西姆芽孢杆菌在防治白腐病中的应用 Download PDF

Info

Publication number
CN108849986A
CN108849986A CN201810744695.2A CN201810744695A CN108849986A CN 108849986 A CN108849986 A CN 108849986A CN 201810744695 A CN201810744695 A CN 201810744695A CN 108849986 A CN108849986 A CN 108849986A
Authority
CN
China
Prior art keywords
garlic
white rot
bacillus
lyceum
biological pesticide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810744695.2A
Other languages
English (en)
Other versions
CN108849986B (zh
Inventor
王军娟
蒋继宏
杨绪勤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Normal University
Original Assignee
Jiangsu Normal University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Normal University filed Critical Jiangsu Normal University
Priority to CN201810744695.2A priority Critical patent/CN108849986B/zh
Publication of CN108849986A publication Critical patent/CN108849986A/zh
Application granted granted Critical
Publication of CN108849986B publication Critical patent/CN108849986B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
    • A01N33/18—Nitro compounds
    • A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
    • A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/34—Nitriles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
    • A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
    • A01N39/04—Aryloxy-acetic acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having no bond to a nitrogen atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A01N47/14—Di-thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

西姆芽孢杆菌(Bacillus siamensis)在防治白腐病中的应用，其通过向大蒜植株茎基部喷洒西姆芽孢杆菌菌液促进大蒜合成蒜氨酸以拮抗大蒜白腐病。本发明还提供了西姆芽孢杆菌在制备防治大蒜白腐病的生物农药中的应用，包括作为活性成分的西姆芽孢杆菌和载体，所述生物农药为促进大蒜产生蒜氨酸的生物农药。以西姆芽孢杆菌防治大蒜白腐病，无农药残留，无环境污染，不对人畜造成安全威胁；以西姆芽孢杆菌的发酵液制备促进大蒜合成蒜氨酸的生物农药，制备过程简单，成本低，效果好，使用方便。

Description

西姆芽孢杆菌在防治白腐病中的应用
技术领域
本发明涉及生物防治与生物农药，尤其涉及西姆芽孢杆菌在防治白腐病中的应用。
背景技术
大蒜(Allium sativum L.)是百合科葱属作物，其以鳞茎(蒜头)、蒜薹和幼株为产品，营养丰富，是最受欢迎的传统调制品和重要的保健食品之一。我国是大蒜的主要生产国、消费国和出口国，大蒜产量约占全球产量的90％，居世界首位。近年来，随着大蒜的规模化种植和产业化发展，鳞茎腐烂一直是引起大蒜减产的主要因素。其中由小核菌属白腐小核菌(Sclerotium cepivorum Berk.)引起的大蒜白腐病是大蒜鳞茎腐烂的主要制约因素。鳞茎受害后变黑腐烂，造成田间缺株死苗，甚至整个地块绝收，从而，直接影响大蒜高产稳产，给蒜农造成很大的经济损失。并且受白腐病菌侵染的新鳞茎，留种后不仅成为田间初侵染源，使危害逐年加重，而且随人为调换蒜种，带菌蒜种又成为异地远距离传播的重要途径，造成该病跨区域发生，大面积流行。化学药剂对白腐病虽然有一定的防治效果，但是没有特效，并且会导致严重的环境问题，降低食用安全性；培育抗病品种缺乏操作性强的抗病品种评价体系，往往在品种育成后才进行抗病性鉴定，导致花费大量人力物力育成的丰产优质品种出现不抗病现象。
生物防治对环境无污染，无残留，并且持续时间长，成本低，是防治大蒜白腐病最经济有效的措施。利用植物内生菌防治植物病害是植物生防领域的研究热点。植物内生菌存活于健康植物组织内而又不引发宿主植物表现出明显感染症状，防治效果稳定，持续时间长。一些内生菌可以产生细菌素、嗜铁素、抗生素吩嗪酸等抗菌物质，抑制病原菌的生长，还有一些是通过产生IAA和类细胞分裂素等植物激素促进植物生长，增强植物自身抗病能力；另外一些是通过促进植物本身的抗病相关物质如超氧化物歧化酶、过氧化物酶的产生而参与到抗病过程的。但是，利用内生菌促进大蒜植株产生大蒜素前体物质-蒜氨酸对白腐病进行生物防治，不仅增加了大蒜植株本身蒜氨酸的含量，而且提高了大蒜植株本身的抗病性，使用方法简便高效，是一项具有开发潜力和开发价值的新型技术。目前国内外对于内生菌促进大蒜植株蒜氨酸的产生，还未有报道，尚未有通过提高大蒜蒜氨酸从而高效抑制白腐病的菌种。
发明内容
为了克服化学农药在防治大蒜白腐病中存在的环境污染、农药残留及威胁人畜健康等问题，本发明提供了西姆芽孢杆菌(Bacillus siamensis)在防治白腐病中的应用，通过向大蒜植株茎基部喷洒西姆芽孢杆菌菌液促进大蒜合成蒜氨酸以拮抗大蒜白腐病，此构成本发明的第一个方面。
本发明还提供了西姆芽孢杆菌在制备防治大蒜白腐病的生物农药中的应用，包括作为活性成分的西姆芽孢杆菌和载体，所述生物农药为促进大蒜产生蒜氨酸的生物农药。此外，所述生物农药还包括复配剂，所述复配剂为多菌灵、甲基硫菌灵、硫菌灵、百菌清、代森锰锌、托布津、春雷霉素、噻呋酰胺和芸苔素中的至少一种，此构成本发明的第二个方面。
本发明的有益效果：以西姆芽孢杆菌防治大蒜白腐病，无农药残留，无环境污染，不对人畜造成安全威胁；以西姆芽孢杆菌的发酵液制备促进大蒜合成蒜氨酸的生物农药，制备过程简单，成本低，效果好，使用方便。
本发明的西姆芽孢杆菌(Bacillus siamensis)为西姆芽孢杆菌Bacillussiamensis KCTC13613(T)，文献出处：Haeyoung J，et al.2012.Draft Genome Sequenceof the Plant Growth-Promoting Bacterium.Genome Announcem.194:4148–4149.
西姆芽孢杆菌Bacillus siamensis KCTC13613(T)可从各地大蒜种植区的植株上通过常规方法分离筛选而获得，也可联系本申请人索取。申请人声明从本申请申请日起二十年内，公众可联系申请人索取该菌种。
附图说明
图1为图1为本发明实施例1所述的菌株153在LB平板上培养24h菌落的照片；
图2为本发明实施例2中所述的在体外菌株153对大蒜白腐病菌菌丝生长的抑制作用照片，其中，图A-1和A-2为菌株153对白腐病菌的抑菌图，图B-1和B-2为白腐病菌空白对照图，A-1和B-1为培养基的反面图，A-2和B-2为培养基正面图；
图3为本发明实施例3中所述的在温室中菌株153对大蒜白腐病抑制作用照片，其中，Control-：未接白腐病菌也未被内生菌处理；Control+：接种白腐病菌但未被内生菌处理；153+：被153菌株菌液处理并接种了白腐病菌；153-：只被153菌株菌液处理；
图4为本发明实施例4中所述的菌株153促进大蒜抵抗白腐菌侵染过程中，大蒜蒜氨酸含量的增加图，其中，Control-：未接白腐病菌也未被内生菌处理；Control+：接种白腐病菌但未被内生菌处理；153+：被153菌株菌液处理并接种了白腐病菌；153-：只被153菌株菌液处理。
具体实施方式：
实施例1：西姆芽孢杆菌(Bacillus siamensis)的菌株鉴定
选择健康且长势良好的大蒜植株，通过常规方法提取内生菌，并分离纯化，编号。其中，编号为153的纯菌株的形态特征如图1所示，菌株153在LB固体平板上生长良好，在37℃条件下培养24h后，菌落为浅黄色，形状为圆形、边缘光滑、直径约2-4mm。使用LB液体培养基于37℃、200rpm摇瓶培养24h后，以8000rpm离心收集菌体，通过微波法提取菌体基因组DNA，用细菌通用引物27F：5'-AGAGTTTGATCCTGGCTCAG-3'和1492R：5'-AAGGAGGTGATCCAGCCGCA-3'进行16S rDNNA基因PCR扩增。PCR反应体系为：10×buffer Mix(包含Taq酶)12.5μL，10μmol/L 27F 0.5μL，10μmol/L 1492R 0.5μL，ddH2O 10.5μL，模板DNA 1μL，PCR扩增产物经0.8％琼脂糖凝胶电泳检测后，送至上海生工有限公司测序，结果如SEQ ID No.1所示。在NCBI以及EzBio Cloud网站上比对测序结果，比对结果如表1所示，该菌株与西姆芽孢杆菌Bacillus siamensis KCTC13613(T)菌株的相似性为99.85％。
表1拮抗菌株153的序列比对结果
实施例2：体外菌株153对大蒜白腐病菌菌丝生长的抑制作用测定
将白腐病菌在PDA培养基上28℃培养5天，153菌株在LB培养基上37℃培养16h，然后挑取单菌落在LB液体培养基中200rpm摇培12h。用打孔器在白腐病菌菌落边缘打出0.5×0.5cm2的菌饼，将有菌丝的一面贴在PDA培养基上放置，同时吸取0.2μL细菌菌液滴入到统一PDA培养基上，25℃对峙培养5天，两种菌体离培养皿边缘的距离都是2cm。对照只在PDA上放置白腐菌菌饼。5天过后，测量对照白腐病菌的直径和与153菌株共培养的白腐病菌的直径，然后根据公式：抑菌率(100％)＝[(对照白腐病菌直径-处理白腐病菌直径)/对照白腐病菌直径]×100算出抑菌率，结果如表2所示。
表2 153菌株对白腐小核菌的抑菌率
从表2中可以看出，153菌株对白腐病菌的抑制率达到24.12％，同时其生物膜的产生能力达到5.69。图2所示为同时共培养5天后，共培养和对照的照片。其中，PDA培养基配方如下：去皮土豆200g；葡萄糖20g；琼脂15g；蒸馏水1000mL；pH自然；LB配方如下：10g胰蛋白胨；5g酵母粉；30g NaCl；琼脂18g；pH 7.0；1000mL蒸馏水。
实施例3：温室中菌株153对大蒜白腐病抑制作用测定
将37℃液体LB摇培16h的153菌液用无菌水洗3次，最终用无菌水将菌液稀释到108个细胞/mL，以供内生菌处理用。从保种管中挑取白腐病菌菌核接种在PDA培养基上，25℃培养5天，以供接菌用。在21×20×15cm3的花盆中播种大蒜品种“改良”的磷茎，25℃光照16h/黑暗8h培养20-25天，进入大蒜花芽磷芽分化期(6叶期)。取17mL 153菌株的菌液，沿着大蒜植株茎基部喷涂一圈，然后覆上土，25℃黑暗保湿24h，然后25℃光照16h/黑暗8h培养3天。用打孔器在白腐菌菌落边缘新生菌丝区取下0.5×0.5cm2的菌饼，将有菌丝的一面贴在大蒜茎基部，覆上土，黑暗保湿24h(湿度60-80％％)，然后25℃光照16h/黑暗8h培养。空白对照不接内生菌也不接白腐病菌，阳性对照为只接153内生菌菌液、只接白腐病菌。每个处理4盆，总共重复了3次试验。从白腐病菌接菌后的第7天开始统计白腐病发病率，发病率由以下公式算出：发病率(100％)＝(出现黄花或者萎蔫的植株数目/总植株数目)×100。计算结果如表3所示，其中，对照-：未接白腐病菌也未被内生菌处理；对照+：接种白腐病菌但未被内生菌处理；153+：被153菌株菌液处理并接种了白腐病菌。
表3 153菌株对白腐病发病率的影响
从表3中可以看出，接种白腐病菌的第11到13天，与只接白腐病菌的对照相比，提前用153菌液喷涂的处理分别降低了50％和40％的发病率。如图3所示，有153菌液处理过的大蒜植株的生长情况与空白对照几乎一样健康，而只有白腐病菌接种的植株出现萎蔫黄花，植株矮小。在白腐病菌接菌后的第13天，对各处理茎基部组织进行采样，用于后续蒜氨酸含量测定。
实施例4：菌株153促进大蒜抵抗白腐饼菌侵染过程中大蒜蒜氨酸含量测定
取实施例3中各处理茎基部的样品0.40g，液氮研磨，转入离心管，加入等体积的磺基水杨酸和超纯水。涡旋仪振荡5-10s混匀。室温静置30min，再混匀30s。然后混合液4℃12000rpm离心20min，然后取上清液用0.22μm水相膜过滤到新的2.0mL离心管中，然后用超纯水稀释20倍，混匀取1mL上氨基酸分析仪(S433D，SYKAM，德国)测定。利用外标法以购买的商品的蒜氨酸标准样品(禾工，上海，中国)做为蒜氨酸的鉴定和定量的标准。色谱条件为：柱型：LCA K07/Li 150mm×4.6mm，洗脱液流速为0.45mL/min。分离柱首先以茚三酮洗脱，流速为0.25mL/min，将柱子进行衍生化作用，然后进样测试样品，蒜氨酸吸收峰为570nm和440nm.定量单位为mg/mL。如图4所示，预先用153菌液处理再接种白腐病菌的大蒜植株(153+)的蒜氨酸含量比其他处理的都高，比空白对照(Control-)、只接153菌液(153-)和只接白腐病菌(Control+)处理中的蒜氨酸含量分别高出了0.22mg、0.24mg、0.14mg。
由于西姆芽孢杆菌与大蒜的共生关系，西姆芽孢杆菌对大蒜的健康生长是有利的，因此，从大蒜中分离内生菌进行富集后在喷施在大蒜上可以帮助植物抵抗病原菌的侵染。西姆芽孢杆菌可与非离子表面活性剂，如吐温、脂肪胺、甘油脂肪酸酯类混合，以水为介质，超微粉碎制成粘稠状可流动的悬浮液，喷洒于作物表面，可吸附于微粒表面形成不同的分散体系，改善植物茎基部生物农药的分布和渗透，提高利用率。其次，也可与惰性填料和一定量的分散剂，按比例经充分混匀后，达到一定粉粒细度。这些粉状农药可以直接施于植物茎基部，被吸收，也可加水湿润、分散，喷洒施用。粉状农药具有储运方便、安全的特点，包装材料容易处理，对植物也较安全。再次，也可以与植物生长调节剂如赤霉素、乙烯利、氯吡脲(环丙酰胺酸)、三十烷醇、苄基腺嘌呤、4-氯苯氧乙酸(防落素、番茄灵)、吲哚乙酸、萘乙酸、胺鲜酯、调环酸、复硝酚钠、芸薹素内酯、油菜素内酯、壳聚糖、芸苔素或广谱杀菌剂如多菌灵、甲基硫菌灵、硫菌灵、百菌清、代森锰锌、托布津、春雷霉素、噻呋酰胺等混合配制达到抗病和生长调节等多重功效。
序列表
<110> 江苏师范大学
<120> 西姆芽孢杆菌在防治白腐病中的应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1349
<212> DNA
<213> 西姆芽孢杆菌(Bacillus siamensis)
<400> 1
agcttgctcc ctgatgttag cggcggacgg gtgagtaaca cgtgggtaac ctgcctgtaa 60
gactgggata actccgggaa accggggcta ataccggatg gttgtctgaa ccgcatggtt 120
cagacataaa aggtggcttc ggctaccact tacagatgga cccgcggcgc attagctagt 180
tggtgaggta acggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc 240
acactgggac tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc 300
aatggacgaa agtctgacgg agcaacgccg cgtgagtgat gaaggttttc ggatcgtaaa 360
gctctgttgt tagggaagaa caagtgccgt tcaaataggg cggcaccttg acggtaccta 420
accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 480
tgtccggaat tattgggcgt aaagggctcg caggcggttt cttaagtctg atgtgaaagc 540
ccccggctca accggggagg gtcattggaa actggggaac ttgagtgcag aagaggagag 600
tggaattcca cgtgtagcgg tgaaatgcgt agagatgtgg aggaacacca gtggcgaagg 660
cgactctctg gtctgtaact gacgctgagg agcgaaagcg tggggagcga acaggattag 720
ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttagggggt ttccgcccct 780
tagtgctgca gctaacgcat taagcactcc gcctggggag tacggtcgca agactgaaac 840
tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 900
gcgaagaacc ttaccaggtc ttgacatcct ctgacaatcc tagagatagg acgtcccctt 960
cgggggcaga gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1020
taagtcccgc aacgagcgca acccttgatc ttagttgcca gcattcagtt gggcactcta 1080
aggtgactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1140
tatgacctgg gctacacacg tgctacaatg gacagaacaa agggcagcga aaccgcgagg 1200
ttaagccaat cccacaaatc tgttctcagt tcggatcgca gtctgcaact cgactgcgtg 1260
aagctggaat cgctagtaat cgcggatcag catgccgcgg tgaatacgtt cccgggcctt 1320
gtacacaccg cccgtcacac cacgagagt 1349

Claims (8)

1.西姆芽孢杆菌(Bacillus siamensis)在防治白腐病中的应用。
2.根据权利要求1所述的西姆芽孢杆菌在防治白腐病中的应用，其特征在于，所述白腐病为大蒜白腐病。
3.根据权利要求2所述的西姆芽孢杆菌在防治白腐病中的应用，其特征在于，其是向大蒜喷洒西姆芽孢杆菌的菌液促进大蒜产生蒜氨酸拮抗所述大蒜白腐病菌。
4.根据权利要求2所述的西姆芽孢杆菌在防治白腐病中的应用，其特征在于，所述西姆芽孢杆菌的菌液的喷洒方法为，将所述西姆芽孢杆菌的菌液喷洒在大蒜植株茎基部。
5.权利要求1中的西姆芽孢杆菌在制备防治大蒜白腐病的生物农药中的应用。
6.根据权利要求5所述的西姆芽孢杆菌在制备防治大蒜白腐病的生物农药的应用，其特征在于，所述生物农药为促进大蒜产生蒜氨酸的生物农药。
7.根据权利要求5所述的西姆芽孢杆菌在制备防治大蒜白腐病的生物农药的应用，其特征在于，所述生物农药包括作为活性成分的西姆芽孢杆菌和载体。
8.根据权利要求5所述的西姆芽孢杆菌在制备防治大蒜白腐病的生物农药的应用，其特征在于，所述生物农药还包括复配剂，所述复配剂为赤霉素、乙烯利、氯吡脲、三十烷醇、苄基腺嘌呤、4-氯苯氧乙酸、吲哚乙酸、萘乙酸、胺鲜酯、调环酸、复硝酚钠、芸薹素内酯、油菜素内酯、壳聚糖、多菌灵、甲基硫菌灵、硫菌灵、百菌清、代森锰锌、托布津、春雷霉素、噻呋酰胺和/芸苔素。
CN201810744695.2A 2018-07-09 2018-07-09 西姆芽孢杆菌在防治白腐病中的应用 Active CN108849986B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810744695.2A CN108849986B (zh) 2018-07-09 2018-07-09 西姆芽孢杆菌在防治白腐病中的应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810744695.2A CN108849986B (zh) 2018-07-09 2018-07-09 西姆芽孢杆菌在防治白腐病中的应用

Publications (2)

Publication Number Publication Date
CN108849986A true CN108849986A (zh) 2018-11-23
CN108849986B CN108849986B (zh) 2020-09-15

Family

ID=64300186

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810744695.2A Active CN108849986B (zh) 2018-07-09 2018-07-09 西姆芽孢杆菌在防治白腐病中的应用

Country Status (1)

Country Link
CN (1) CN108849986B (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362787A (zh) * 2018-12-07 2019-02-22 江苏师范大学 暹罗芽胞杆菌在防治线虫中的应用
WO2021178384A1 (en) * 2020-03-04 2021-09-10 Tu Biomics, Inc. Compositions and methods for inhibiting a fungal pathogen

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103215210A (zh) * 2013-05-08 2013-07-24 中国海洋大学生物工程开发有限公司 一种西姆芽孢杆菌及其在制备微生物海藻肥料中的应用
CN104342394A (zh) * 2014-10-31 2015-02-11 中国农业科学院农产品加工研究所 一株暹罗芽孢杆菌及其在防治禾谷镰刀菌方面的应用
CN105505834A (zh) * 2016-01-25 2016-04-20 北京市辐射中心 一株防治梨轮纹病和软腐病的暹罗芽孢杆菌及其应用
CN106282067A (zh) * 2016-08-24 2017-01-04 李丽丽 多功能农用复合微生物菌剂及微生态制剂与应用
CN106867934A (zh) * 2017-02-15 2017-06-20 昆明市农业科学研究院 一株暹罗芽孢杆菌及其在防治植物白粉病中的应用
CN107034158A (zh) * 2017-04-27 2017-08-11 中国科学院微生物研究所 暹罗芽孢杆菌b‑3及其应用

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103215210A (zh) * 2013-05-08 2013-07-24 中国海洋大学生物工程开发有限公司 一种西姆芽孢杆菌及其在制备微生物海藻肥料中的应用
CN104342394A (zh) * 2014-10-31 2015-02-11 中国农业科学院农产品加工研究所 一株暹罗芽孢杆菌及其在防治禾谷镰刀菌方面的应用
CN105505834A (zh) * 2016-01-25 2016-04-20 北京市辐射中心 一株防治梨轮纹病和软腐病的暹罗芽孢杆菌及其应用
CN106282067A (zh) * 2016-08-24 2017-01-04 李丽丽 多功能农用复合微生物菌剂及微生态制剂与应用
CN106867934A (zh) * 2017-02-15 2017-06-20 昆明市农业科学研究院 一株暹罗芽孢杆菌及其在防治植物白粉病中的应用
CN107034158A (zh) * 2017-04-27 2017-08-11 中国科学院微生物研究所 暹罗芽孢杆菌b‑3及其应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
欧阳慧: "抗猕猴桃果腐病西姆芽孢杆菌的分离鉴定及抑菌活性的初步研究", 《江西农业大学硕士学位论文》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362787A (zh) * 2018-12-07 2019-02-22 江苏师范大学 暹罗芽胞杆菌在防治线虫中的应用
WO2021178384A1 (en) * 2020-03-04 2021-09-10 Tu Biomics, Inc. Compositions and methods for inhibiting a fungal pathogen

Also Published As

Publication number Publication date
CN108849986B (zh) 2020-09-15

Similar Documents

Publication Publication Date Title
CN102428966B (zh) 一种作物病害防治复合生物制剂及其应用
CN103865843B (zh) 一种微生物复合菌及其在防治蔬菜真菌病害中的应用
CN102391876B (zh) 一种复合生物土壤改良剂及其应用
CN110483111A (zh) 一种防治土传病害的生物有机肥及制备方法及应用
CN106172507B (zh) 枯草芽孢杆菌hmb19198在防治黄瓜白粉病上的应用
CN103045506B (zh) 一种连作障碍自毒物质降解菌及其制备的复合微生物肥料
CN106929443B (zh) 一种弧菌lx6-2及其在制备生物海藻肥中的用途
CN113373180B (zh) 解淀粉芽孢杆菌的纳米硒合成活性菌液、其制备方法及应用
CN107245461A (zh) 一种解淀粉芽孢杆菌b6及其应用
CN103651582A (zh) 广谱复合微生物杀菌剂及其制备方法
CN108849986A (zh) 西姆芽孢杆菌在防治白腐病中的应用
CN113832060B (zh) 一种抗重茬微生物菌剂及其在农业生产中的应用
CN105638651A (zh) 一种萎缩芽孢杆菌可湿性粉剂的制备方法
CN102391040A (zh) 一种新型有机微生物菌肥及其制备工艺
US11674118B2 (en) PGPR compositions and methods for improved cultivation of tomato and potato species
JP2006124280A (ja) 植物種子発芽率向上剤
CN114250177B (zh) 一株不动杆菌及其在提高植物抗逆性中的应用
CN109402004A (zh) 一株简氏气单胞菌及其在提高植物抗逆性中的应用
CN107227270A (zh) 一种土传病害生防菌剂、一种土传病害生物防治基质及其制备方法和使用方法
CN110358698B (zh) 巨大芽孢杆菌在增强植物抗干旱胁迫能力中的应用
CN111909863B (zh) 一株解淀粉芽孢杆菌及其用途
CN106967654A (zh) 一种甲基营养型芽孢杆菌及生物复合种衣剂和应用
CN103525729B (zh) 嗜硫小红卵菌菌株、菌剂及其应用
CN112899206A (zh) 一株产几丁质酶、产吲哚乙酸的芽孢杆菌及其应用和方法
CN111944710A (zh) 一株希瓦氏菌及其在提高植物抗逆性中的应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20181123

Assignee: JIANGSU NATURE BIOTECHNOLOGY Co.,Ltd.

Assignor: Jiangsu Normal University

Contract record no.: X2021980009278

Denomination of invention: Application of Bacillus Sims in the control of white rot

Granted publication date: 20200915

License type: Common License

Record date: 20210913

EE01 Entry into force of recordation of patent licensing contract