CN108849476A - Improve the breeding method that oil uses tree peony drought resistance - Google Patents
Improve the breeding method that oil uses tree peony drought resistance Download PDFInfo
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- CN108849476A CN108849476A CN201810769231.7A CN201810769231A CN108849476A CN 108849476 A CN108849476 A CN 108849476A CN 201810769231 A CN201810769231 A CN 201810769231A CN 108849476 A CN108849476 A CN 108849476A
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- seed
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- peony
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
Abstract
The invention belongs to oil tree peony breeding technical fields, and in particular to a kind of breeding method for improving oil and using tree peony drought resistance.The excellent oil of this method selection traits uses Varieties of Peony as female parent, selects the oil of stable yields to use Varieties of Peony as male parent, hybridizes and harvest first generation of hybrid seed F1, plant F1And mixed receipts, obtain second generation of hybrid seed F2, F2Single plant and maternal backcrossing, obtain first backcross generation seed BC1, BC1Single plant is returned with female parent again, obtains second backcross generation seed BC2, finally plant second backcross generation seed BC2, 3~4 generations are selfed, select best strain as oil tree peony new varieties；Period carries out water stress processing using tightening agent, can strangle the sprouting of not drought-enduring seed in cradle, reduces the generation of false positive drought resisting seed, reduces total sample size of subsequent hybridization step, reduces workload, improves breeding efficiency.
Description
Technical field
The invention belongs to tree peony breeding technical fields, and in particular to a kind of breeding method for improving oil and using tree peony drought resistance.
Background technique
Wild tree peonies kind in China's is mainly distributed on the ground such as Henan, Shaanxi, Hubei, Gansu, Sichuan, Yunnan, Tibet at present.
According to incompletely statistics, there is ability about more than 100 kinds.Oil with tree peony refer to sub real yield is high, oil content is high, effectively at
Divide high Varieties of Peony type.Oil common at present is mainly phoenix pellet tree peony and Paeonia papaveracea two major classes with tree peony.Phoenix pellet tree peony
It is more than 90% with unsaturated fatty acid content in paeonia rockii seed, wherein linolenic acid content accounts for 40% or so.Linolenic acid is manpower
Essential amino acid has and refreshes the mind, enhances memory, reducing blood lipid, blood pressure lowering, prevention cardiovascular and cerebrovascular disease and other effects, to the mankind
Health plays key effect.Less in view of the oil Varieties of Peony of suitable plantation and stable yields, therefore, this kind of tree peony of Devoting Major Efforts To Developing is new
Kind is of great significance.
Traditional crossbreeding mode relies on the combination of excellent genes under natural conditions, can select character and stablize heredity
Improved Varieties.Parent that drought resistant plant variety breeding is typically chosen itself must have preferable drought resistance, after hybridization
Generation plantation and Identification of Drought selection under natural environmental condition.But due to being suitble to the oil of plantation and stable yields, drought resisting to use tree peony
It is of less types, the breeding of natural hybridization mode is only leaned on, the probability for generating new varieties is small, and breeding efficiency is low.
Summary of the invention
A kind of breeding method for improving oil and using tree peony drought resistance provided by the invention, by conventional hybridization mode and chemical reagent
Processing method combines, and reduces the generation of false positive drought resisting seed, reduces total sample size of subsequent hybridization step, reduces work
Amount, improves breeding efficiency.
The object of the present invention is to provide a kind of breeding methods for improving oil and using tree peony drought resistance, include the following steps：
S1, the excellent oil of selection traits use Varieties of Peony as maternal, and the oily of stable yields is selected to use Varieties of Peony as male parent,
By male parent and hybridization of female parent, first generation of hybrid seed F is harvested1；
S2, to first generation of hybrid seed F1Water stress processing is carried out, and will treated F1It plants in big Tanaka, selectivity
The excellent plant seed of shape is mixed to be received, and second generation of hybrid seed F is obtained2；
S3, to second generation of hybrid seed F2Carry out water stress processing, and by the female parent selected in S1 and treated F2Plantation
In big Tanaka, selection traits are excellent and the F of drought resisting2Single plant is returned with maternal in S1 as new male parent, obtains first backcross generation kind
Sub- BC1；
S4, to first backcross generation seed BC1Carry out water stress processing, and by the female parent selected in S1 and treated BC1Kind
It plants in big Tanaka, selection traits are excellent and the BC of drought resisting1Single plant is returned with maternal in S1 as new male parent, obtains backcrossing two
For seed BC2；
S5 plants second backcross generation seed BC2, 3~4 generations are selfed, selection traits are stable, excellent and drought resisting oil is with male
Pellet forms several strains, carries out field trial to the strain of formation, selects best strain as oil tree peony new varieties；
In S2~S4, water stress processing the step of it is as follows：
Oil is soaked in 2~8h in tightening agent with Paeonia suffruticosa seed, blots oil Paeonia suffruticosa seed surface stress after taking out seed
Agent, then be soaked in 5~10min in glycerol drains after taking out seed, the seed that obtains that treated；Wherein, the tightening agent be by
What the raw material of following mass percent was formulated：35~60% PEG6000,1~10% stimulating factor, surplus are sterile
Water；The stimulating factor is one of soluble calcium salt, sodium salt, sylvite or two kinds any.
Preferably, the breeding method of above-mentioned raising oil tree peony drought resistance, the soluble calcium salt are calcium chloride, glucose
The one or more of sour calcium, calcium dihydrogen phosphate.
Preferably, the breeding method of above-mentioned raising oil tree peony drought resistance, when containing two or more calcium salts in tightening agent,
Calcium salt total amount is 1~8%.
Preferably, the breeding method of above-mentioned raising oil tree peony drought resistance, the sodium salt be sodium chloride, disodium hydrogen phosphate,
The one or more of sodium dihydrogen phosphate.
Preferably, the breeding method of above-mentioned raising oil tree peony drought resistance, the sylvite are potassium chloride, potassium nitrate, phosphoric acid
The one or more of potassium dihydrogen, dipotassium hydrogen phosphate.
Compared with prior art, the breeding method provided by the invention for improving oil and using tree peony drought resistance, has beneficial below
Effect：
The present invention is experiment proves that prove, the tightening agent of special formulation of the present invention and corresponding water stress processing method pair
The oil seed of tree peony is sprouted and plant strain growth has certain influence, is affected to the general kind of drought-resistant ability, can will not
The sprouting of drought-enduring seed is strangled in cradle, and the generation of false positive drought resisting seed is reduced.
The present invention successively uses tightening agent immersion and glycerol to impregnate two steps, and cell interior is broken in wherein tightening agent immersion
The balance of substance inhibits the germination and growth of seed；Glycerol has adsorptivity, and a small amount of glycerol is adhered to the surface of the seed, in seed
Sprouting initial stage has obstructed exchanging for substance and external environment in seed cell, limits neutron absorption nutrition, also slows down in tightening agent
Each ion from seed cell dissolve out, using glycerol extend tightening agent effect, barrier external nourishment substance enter seed cell,
It influences oil and grows germination with Paeonia suffruticosa seed, eliminate not drought-enduring seed in advance, reduce total sample size of subsequent hybridization step, reduce
Workload improves breeding efficiency.
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but should not be construed as limitation of the invention.It is following
The test method of actual conditions is not specified in embodiment, operates usually according to normal condition, due to not being related to inventive point, thus it is not right
Its step is described in detail.
A kind of breeding method improving oily tree peony drought resistance provided by the invention includes following embodiment.
Embodiment 1
A kind of breeding method for improving oil and using tree peony drought resistance, includes the following steps：
S1 selects the oil of stable yields drought resisting to use Varieties of Peony Paeonia papaveracea (avenging extra large loyal heart) as female parent, and selection traits are excellent
Oil use Varieties of Peony phoenix pellet tree peony (Feng Danbai) as male parent, by male parent and hybridization of female parent, harvest first generation of hybrid seed F1；
S2, to first generation of hybrid seed F1Water stress processing is carried out, and will treated F1It plants in big Tanaka, selection length
The excellent plant seed of the good character of gesture is mixed to be received, and second generation of hybrid seed F is obtained2；
S3, to second generation of hybrid seed F2Water stress processing is carried out, and the female parent selected in S1 is avenged into extra large loyal heart and processing
F afterwards2It plants and selects the good character of growing way excellent in big Tanaka and the F of drought resisting2Single plant is as new male parent, with snow maternal in S1 sea
Loyal heart backcrossing, obtains first backcross generation seed BC1；
S4, to first backcross generation seed BC1Water stress processing is carried out, and the female parent selected in S1 is avenged into extra large loyal heart and processing
BC afterwards1It plants and selects the good character of growing way excellent in big Tanaka and the BC of drought resisting1Single plant is avenged as new male parent with maternal in S1
Extra large loyal heart backcrossing, obtains second backcross generation seed BC2；
S5 plants second backcross generation seed BC2, it is selfed 4 generations, selection traits are stable, excellent and drought resisting oil is with tree peony,
10 strains are formed, field trial identification is carried out to 10 strains of formation, select best strain as oil tree peony new varieties.
In S2~S4, water stress processing the step of it is as follows：
Oil is soaked in 2h in tightening agent with Paeonia suffruticosa seed, blots oil Paeonia suffruticosa seed surface tightening agent after taking out seed,
It is soaked in 5min in glycerol again, takes out and drain after seed (be subject to no glycerol under drip), the seed that obtains that treated；Wherein,
The tightening agent is formulated by the raw material of following mass percent：It is the stimulating factor of 50% PEG 6000,10%, remaining
Amount is sterile water；The stimulating factor is calcium chloride.
Embodiment 2
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 1, difference is, the stimulation
The factor is sodium chloride.
Embodiment 3
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 1, difference is, the stimulation
The factor is potassium chloride.
Embodiment 4
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 1, difference is, in S2~S4,
The step of water stress processing, is as follows：
Oil is soaked in 8h in tightening agent with Paeonia suffruticosa seed, blots oil Paeonia suffruticosa seed surface tightening agent after taking out seed,
It is soaked in 10min in glycerol again, takes out and drain after seed (be subject to no glycerol under drip), the seed that obtains that treated；Wherein,
The tightening agent is formulated by the raw material of following mass percent：It is the stimulating factor of 35% PEG 6000,5%, remaining
Amount is sterile water；The stimulating factor is calcium gluconate and sodium dihydrogen phosphate according to 1:The mixture of 1 mass ratio.
Embodiment 5
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 4, difference is, the stimulation
The factor is calcium dihydrogen phosphate and potassium nitrate according to 1:The mixture of 2 mass ratios；It was 3 generations that algebra is selfed in S5.
Embodiment 6
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 4, difference is, the stimulation
The factor is calcium dihydrogen phosphate, sodium dihydrogen phosphate and potassium nitrate according to 2:1:The mixture of 1 mass ratio.
Embodiment 7
A kind of breeding method for improving oil and using tree peony drought resistance, substantially the same manner as Example 1, difference is, in S2~S4,
The step of water stress processing, is as follows：
Oil is soaked in 4h in tightening agent with Paeonia suffruticosa seed, blots oil Paeonia suffruticosa seed surface tightening agent after taking out seed,
It is soaked in 8min in glycerol again, the seed that obtains that treated；Wherein, the tightening agent is matched by the raw material of following mass percent
Made of system：The stimulating factor of 60% PEG 6000,8%, surplus are sterile water；The stimulating factor be calcium gluconate,
Calcium dihydrogen phosphate, potassium dihydrogen phosphate and sodium dihydrogen phosphate are according to 1:1:1:The mixture of 1 mass ratio.
In order to verify effect of the invention, We conducted following experiments.
One, oil is tested with tree peony drought resistance
From the S1 first generation of hybrid seed F of embodiment 11In randomly select 1400g, be divided into example 1 group, embodiment 2
Group, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, 7 groups of embodiment, every group of 200g.The experiment of example 1 group
Method is as follows：It mixes according to the method for embodiment 1 and receives F1For seed, F is then handled using the water stress processing method of embodiment 11
For seed, seed after the processing of acquisition.It takes seed after 200 processing to sprout in growth cabinet at random, calculates seed and sprout
Nutrient solution is replaced with mass fraction 10%, mass fraction 20%, mass fraction 25% after germination by rate respectively
The plant of PEG6000 aqueous solution, culture observation 15d is averaged plant height.
2~7 groups of embodiment similar to example 1 group processing mode, and difference is, water stress processing method uses each reality
Apply the corresponding method of example.
The experimental method of experimental group is as follows：It mixes according to the method for embodiment 1 and receives F1For seed, 200 F are taken at random1For seed
It is sprouted in growth cabinet, calculates seed germination rate and nutrient solution is replaced with into mass fraction respectively after germination
10%, in the PEG6000 aqueous solution of mass fraction 20%, mass fraction 25%, the plant of culture observation 15d is averaged plant height.
The above each group is all provided with control experiment, and PEG6000 aqueous solution is changed to distilled water.
Table 1 is each group oil tree peony drought resistance Tests Seeds germination rate, from the results shown in Table 1, due to experimental group
Without water stress processing, so its germination rate is higher, up to 98.7%, and the germination rate of Examples 1 to 7 group is below
80%.
1 each group oil of table tree peony drought resistance Tests Seeds germination rate
Table 2 is each group oil tree peony drought resistance experimental plants height, and the data in longitudinal comparison table 2 can be seen that implementation
1~7 group of example has carried out water stress processing respectively, limits the growth of seed, and plant height is less than experimental group, illustrates the side of body of the invention
The growth of peony plant, sprouting of the Examples 1 to 7 group in table 1 can be limited by compeling agent and corresponding water stress processing method
Rate is lower, has excluded not drought resisting seed.Data in lateral comparison table 2 can be seen that, in the same set, tree peony 10%,
20%, the growth in 25% PEG6000 aqueous solution is affected, through PEG6000 aqueous solution Stress treatment plant mean height
Degree is less than without PEG6000 aqueous solution Stress treatment.
2 each group oil of table tree peony drought resistance experimental plants average height
Two, different in moisture Stress treatment method uses oil the influence of tree peony
The present invention successively uses tightening agent immersion and glycerol to impregnate two steps, and cell interior is broken in wherein tightening agent immersion
The balance of substance inhibits the germination and growth of seed；Glycerol has adsorptivity, and a small amount of glycerol is adhered to the surface of the seed, in seed
Sprouting initial stage has obstructed exchanging for substance and external environment in seed cell, limits neutron absorption nutrition, also slows down in tightening agent
Each ion from seed cell dissolve out, extend tightening agent stress time, as time go on, glycerol is gradually dissolved in culture substrate
In, peony plant root system also gradually grows up to, at this time its can normal growth, be mainly with the inventive method seed stage exclude
Not drought resisting seed.
F is harvested according to the method for embodiment 11It for seed, is soaked in Examples 1 to 7 tightening agent after 2h respectively, takes out kind
Oil Paeonia suffruticosa seed surface tightening agent is blotted after son, seed carries out seed sprouting experiment in growth cabinet after processing, calculates
Normal germination rate, i.e., the germinative number of 100 seeds, the germination rate of Examples 1 to 7 tightening agent processing group is followed successively by 82.3%,
85.7%, 85.3%, 82.4%, 85.9%, 83.4%, 84.6%, the results show that in the condition for lacking glycerol immersion treatment
Under, seed germination rate is higher than above-mentioned 1 each group of data of table.Seed stage is the more fragile time, extends stress using glycerol at this moment
The effect of agent, and obstruct external nourishment substance and enter seed cell, its growth germination is influenced, eliminates not drought-enduring seed in advance,
Total sample size of subsequent hybridization step is reduced, workload is reduced, improves breeding efficiency.
Three, the influence of tree peony is used oil in Different stress agent
F is harvested according to the method for embodiment 11For seed, it is soaked in 6000 aqueous solution of PEG of mass fraction 50% respectively
It after middle 2h, takes out and blots oil after seed with Paeonia suffruticosa seed surface moisture, seed carries out seed in growth cabinet and sprouts after processing
Hair experiment, calculates normal germination rate, i.e., the germinative number of 100 seeds, carries out 5 parallel laboratory tests, germination rate 82.4%,
83.7%, 82.7%, 85.0%, 85.4%, although the results show that 6000 aqueous solution of PEG of high concentration can be to a certain degree
The upper germination for inhibiting seed, but still it is higher than the germination rate of Examples 1 to 7 group in table 1, illustrate single factor test function and effect difference and implements
The germination rate of example 1~7.
It should be noted that involved in the present invention when numberical range, it is thus understood that two endpoints of each numberical range with
And any one numerical value can be selected between two endpoints, repeat in order to prevent, description of the invention preferred embodiment and
Effect, although preferred embodiments of the present invention have been described, once a person skilled in the art knows substantially creative
Concept, then additional changes and modifications can be made to these embodiments.So it includes preferred that the following claims are intended to be interpreted as
Embodiment and all change and modification for falling into the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (5)
1. a kind of breeding method for improving oil and using tree peony drought resistance, which is characterized in that include the following steps：
S1, the excellent oil of selection traits use Varieties of Peony as female parent, select the oil of stable yields to use Varieties of Peony as male parent, by father
Sheet and hybridization of female parent harvest first generation of hybrid seed F1；
S2, to first generation of hybrid seed F1Water stress processing is carried out, and will treated F1It plants in big Tanaka, selection traits are excellent
Good plant seed is mixed to be received, and second generation of hybrid seed F is obtained2；
S3, to second generation of hybrid seed F2Carry out water stress processing, and by the female parent selected in S1 and treated F2It plants in big
Tanaka, selection traits are excellent and the F of drought resisting2Single plant is returned with maternal in S1 as new male parent, obtains first backcross generation seed
BC1；
S4, to first backcross generation seed BC1Carry out water stress processing, and by the female parent selected in S1 and treated BC1Plant in
Big Tanaka, selection traits are excellent and the BC of drought resisting1Single plant is returned with maternal in S1 as new male parent, obtains second backcross generation kind
Sub- BC2；
S5 plants second backcross generation seed BC2, 3~4 generations are selfed, selection traits are stable, excellent and drought resisting oil tree peony, shape
At several strains, field trial is carried out to the strain of formation, selects best strain as oil tree peony new varieties；
In S2~S4, water stress processing the step of it is as follows：
Oil is soaked in 2~8h in tightening agent with Paeonia suffruticosa seed, blots oil Paeonia suffruticosa seed surface tightening agent after taking out seed, then
It is soaked in 5~10min in glycerol, is drained after taking out seed, the seed that obtains that treated；Wherein, the tightening agent is by following
What the raw material of mass percent was formulated：35~60% PEG6000,1~10% stimulating factor, surplus are sterile water；
The stimulating factor is one of soluble calcium salt, sodium salt, sylvite or two kinds any.
2. the breeding method according to claim 1 for improving oil and using tree peony drought resistance, which is characterized in that the solubility calcium
Salt is the one or more of calcium chloride, calcium gluconate, calcium dihydrogen phosphate.
3. the breeding method according to claim 2 for improving oil and using tree peony drought resistance, which is characterized in that contain in tightening agent
When two or more calcium salts, calcium salt total amount is 1~8%.
4. the breeding method according to claim 1 for improving oil and using tree peony drought resistance, which is characterized in that the sodium salt is chlorine
Change the one or more of sodium, disodium hydrogen phosphate, sodium dihydrogen phosphate.
5. the breeding method according to claim 1 for improving oil and using tree peony drought resistance, which is characterized in that the sylvite is chlorine
Change the one or more of potassium, potassium nitrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111034546A (en) * | 2019-12-23 | 2020-04-21 | 云南素麻生物科技有限公司 | Breeding method of industrial cannabis with high CBD content |
| CN111820238A (en) * | 2020-06-09 | 2020-10-27 | 扬州大学 | Drought-resistant agent for relieving drought stress of paeonia ostii and application thereof |
| CN114568297A (en) * | 2022-03-23 | 2022-06-03 | 河南省农业科学院经济作物研究所 | Drought-resistant cotton breeding method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111034546A (en) * | 2019-12-23 | 2020-04-21 | 云南素麻生物科技有限公司 | Breeding method of industrial cannabis with high CBD content |
| CN111820238A (en) * | 2020-06-09 | 2020-10-27 | 扬州大学 | Drought-resistant agent for relieving drought stress of paeonia ostii and application thereof |
| CN114568297A (en) * | 2022-03-23 | 2022-06-03 | 河南省农业科学院经济作物研究所 | Drought-resistant cotton breeding method and application |
| CN114568297B (en) * | 2022-03-23 | 2023-04-18 | 河南省农业科学院经济作物研究所 | Drought-resistant cotton breeding method and application |
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