CN108836564A - A kind of fertilization method improving farrowing efficiency - Google Patents
A kind of fertilization method improving farrowing efficiency Download PDFInfo
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- CN108836564A CN108836564A CN201810687706.8A CN201810687706A CN108836564A CN 108836564 A CN108836564 A CN 108836564A CN 201810687706 A CN201810687706 A CN 201810687706A CN 108836564 A CN108836564 A CN 108836564A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
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Abstract
The present invention relates to cattle breeding technical fields, more particularly to a kind of fertilization method for improving farrowing efficiency, the technical problem to be solved is that be to provide a kind of fertilization method for improving farrowing efficiency, to solve previous fertilization method existing defects, the technical issues of farrowing rate can not be significantly improved, include the following steps：1. 2. 3. 4. sperm motility rate and density inspection judgement determine effective sperm, mixing, dilution, bottling 5. artificial insemination to the preparation before semen collection for semen collection, the present invention is widely used in boar breeding technical field.
Description
Technical field
The present invention relates to cattle breeding technical fields, and in particular to a kind of fertilization method for improving farrowing efficiency.
Background technique
The technology of the artificial insemination of pig starts from the 1930s, having there is 70 years history, and China starts from last century 50
Age, but the ability rapid proliferation after the nineties, the technology has become one of the main means of pig breeding breeding at present, pig
Artificial insemination can be reduced transmission, improves production efficiency, obtains higher economic benefit.
Currently, most pig farms be using natural breeding, i.e., when oestrus of sow is motionless, catch up with boar and sow mating by
Expenditure pregnant, that boar is caught up with, it is low to will cause sperm survival rate, and dead essence rate is high, and conception rate and farrowing rate is caused to decline；In addition, natural
Mating is easy to cause the propagation of bacterium, virus, increases the probability of the infectious disease of sow；Furthermore natural mating mode, one
Breeding boar is once only capable of one sow of mating, and sperm utilization rate is lower, in 1 year a boar at most mate 100-150 it is female
Pig, in order to meet the needs of breeding, the cultivation amount of boar is big, and higher cost, economic benefit is lower, currently, also there is part cultivation
Field is bred using Artificial insemination procedures, but since technology is not mature enough, causes pregnancy rate and farrowing rate lower, economic benefit
It is not significant enough.
Summary of the invention
Based on the above technical problem, the present invention provides a kind of fertilization method for improving farrowing efficiency, to solve previous
Fertilization method existing defects can not significantly improve the technical issues of farrowing rate.
In order to solve the above-mentioned technical problem, the technical scheme adopted by the invention is as follows：A kind of fertilization improving farrowing efficiency
Method includes the following steps：
1. the preparation before semen collection：Distilled water 500ml, dilution powder 25g are placed in 1000ml beaker, stirring and dissolving is heated up to 37
DEG C, constant temperature is spare；Upper disposable special plastic bag is covered on the collection essence cup of 500ml, and covers 3 layers of filter paper, then uses cushion rubber
Filtering paper edge is fixed on rim of a cup, makes to be located inside beaker in the middle part of filter paper；
2. semen collection：Semen collection is carried out to three of the right age boars respectively, semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterile glove,
After boar, which climbs up phantom, stretches out penis, penis front end spire is held with the right hand, and with thumb top glans penis, then pass through
Rhythmically pinching and penis being pulled back and forth, boar being made to generate pleasant sensation and ejaculate, sow and index finger are slightly opened into dew when ejaculation
Glans penis out allows sperm to project, and the thin sperm without sperm projected at first is not collected, and is rich in the milky white of sperm wait project
It is just combined in cup with semen collection cup when color sperm, when there is fibrin clot, exhausts the first time sperm that boar shoots away with thumb, so
The right hand still rhythmically pinching and pulls penis afterwards, with stimulate boar penis continue second and third time ejaculate, boar is penetrated
Essence terminate to back off in advance dummy when, the right hand gently takes advantage of a situation and send penis to foreskin, and semen collection terminates, three of the right age boar semens point
It Wei not sperm I, sperm II and sperm III；
3. sperm motility rate and density inspection judgement：The sperm adopted is checked to the motility rate and density of sperm under the microscope, is judged
Whether sperm can be used, and determine the extension rate of sperm, and concrete operations are as follows：
The judgement of a motility rate：Collected sperm is dripped and is observed in being placed on glass slide under 400 power microscopes, by straight in the visual field
The percentage of the relative number of the sperm of line forward travel and sperm sum determines the vigor of sperm, is generally commented with reagent
Fixed, 100% sperm straight ahead movement is chosen as level-one motility rate, and 90% sperm straight ahead movement is chosen as 0.9 grade of motility rate, with this
Analogize, 10% sperm straight ahead movement is chosen as 0.1 grade of motility rate, and the sperm of linear forward travel is known as effective sperm of being fertilized；
The judgement of b sperm concentration：Ocular estimate judges rating by the density of sperm intuitive in the visual field, is divided under the microscope
It is close, in, it is dilute third；
It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, contains essence in every milliliter of sperm
Son is about 300,000,000 or more；
In：There are many sperm seen in the visual field, but have apparent gap between sperm and sperm, between sperm away from
Length from about 1 sperm, while single sperm motility is it is clear that contain sperm about 1-3 hundred million in every milliliter of sperm；
It is dilute：A small number of sperms are seen in the visual field, gap is very big to each other for sperm, and gap is more than the length of two sperms, and every milliliter
In sperm containing sperm count about below 100,000,000；
4. determining effective sperm, mixing, dilution, bottling：Sperm survival rate be 0.9 grade or 1 grade, and density be it is close or in sperm
For effective sperm, by effective sperm I, then sperm II and sperm III mixing press 1 with dilution:The mixing of 3-4 dilution proportion is equal
After even, every bottle of 70ml bottling is saved backup, then by spare sperm in foam plastic reservoir slow cooling 15 minutes, dropped to temperature
When to 17-18 DEG C, it is put into 17.2 DEG C of constant temperature refrigerator and saves, sperm bottle is gently overturn 3-5 times every 12 hours, makes sperm
It is sufficiently mixed with dilution, prevents Sperm pellets and cause death；It can be reserved for 10 days in 17.2 DEG C of constant temperature；
5. artificial insemination：The spare sperm of 70ml is inputted with sow of the vas deferens to heat, after 8 hours, by the spare sperm of 70ml
It is placed in 25 DEG C of warm water after heating 5-10min, is incorporated 2ml oxytocin, shakes up, second of semen deposition is carried out to sow, can be improved
Sow number of eggs ovulated and litter size.
As a kind of preferred fertilization method for improving farrowing efficiency, the heat sow is the heat mother to wean 5-6 days
Pig.
As a kind of preferred fertilization method for improving farrowing efficiency, when artificial insemination of the present invention, heat sow exists
Its genital tract is cleaned before first time semen deposition.
As described above, compared with the prior art, the invention has the beneficial effects that：The present invention is respectively to three of the right age public affairs
Pig carries out semen collection, and three kinds of effective sperm are mixed, and carries out artificial insemination to heat sow, effectively improves sow pregnancy rate and farrowing
Rate；The present invention uses unarmed semen collection method, and boar can be made all to project sperm, effectively raise the utilization rate of boar semen,
The cultivation head number for reducing boar, reduces the aquaculture cost of raiser, to improve the economic benefit of raiser；By accurate
The density and survival rate for judging sperm, it is effective that fertilization success rate and farrowing rate are provided；Using artificial insemination, boar can be overcome
Body with the small inconvenient contradiction that mates of sow body, can also prevent the mutual infection of disease greatly；The method of the invention conception rate is high,
Farrowing rate is high, improves the economic benefit of raiser.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
A kind of fertilization method improving farrowing efficiency, includes the following steps：
1. the preparation before semen collection：Distilled water 500ml, dilution powder 25g are placed in 1000ml beaker, stirring and dissolving is heated up to 37
DEG C, constant temperature is spare；Upper disposable special plastic bag is covered on the collection essence cup of 500ml, and covers 3 layers of filter paper, then uses cushion rubber
Filtering paper edge is fixed on rim of a cup, makes to be located inside beaker in the middle part of filter paper；
2. semen collection：Semen collection is carried out to three of the right age boars respectively, semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterile glove,
After boar, which climbs up phantom, stretches out penis, penis front end spire is held with the right hand, and with thumb top glans penis, then pass through
Rhythmically pinching and penis being pulled back and forth, boar being made to generate pleasant sensation and ejaculate, sow and index finger are slightly opened into dew when ejaculation
Glans penis out allows sperm to project, and the thin sperm without sperm projected at first is not collected, and is rich in the milky white of sperm wait project
It is just combined in cup with semen collection cup when color sperm, when there is fibrin clot, exhausts the first time sperm that boar shoots away with thumb, so
The right hand still rhythmically pinching and pulls penis afterwards, with stimulate boar penis continue second and third time ejaculate, boar is penetrated
Essence terminate in advance back off dummy when, the right hand gently takes advantage of a situation and send penis to foreskin, and semen collection terminates, and sperm is respectively sperm I, essence
Liquid II and sperm III；
3. sperm motility rate and density inspection judgement：The sperm adopted is checked to the motility rate and density of sperm under the microscope, is judged
Whether sperm can be used, and determine the extension rate of sperm, and concrete operations are as follows：
The judgement of a motility rate：Collected sperm is dripped and is observed in being placed on glass slide under 400 power microscopes, by straight in the visual field
The percentage of the relative number of the sperm of line forward travel and sperm sum determines the vigor of sperm, is generally commented with reagent
Fixed, 100% sperm straight ahead movement is chosen as level-one motility rate, and 90% sperm straight ahead movement is chosen as 0.9 grade of motility rate, with this
Analogize, 10% sperm straight ahead movement is chosen as 0.1 grade of motility rate, and the sperm of linear forward travel is known as effective sperm of being fertilized；
The judgement of b sperm concentration：Ocular estimate judges rating by the density of sperm intuitive in the visual field, is divided under the microscope
It is close, in, it is dilute third；
It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, contains essence in every milliliter of sperm
Son is about 300,000,000 or more；
In：There are many sperm seen in the visual field, but have apparent gap between sperm and sperm, between sperm away from
Length from about 1 sperm, while single sperm motility is it is clear that contain sperm about 1-3 hundred million in every milliliter of sperm；
It is dilute：A small number of sperms are seen in the visual field, gap is very big to each other for sperm, and gap is more than the length of two sperms, and every milliliter
In sperm containing sperm count about below 100,000,000；
4. determining effective sperm, mixing, dilution, bottling：Sperm survival rate be 0.9 grade or 1 grade, and density be it is close or in sperm
For effective sperm, by effective sperm I, then sperm II and sperm III mixing press 1 with dilution:The mixing of 3-4 dilution proportion is equal
After even, every bottle of 70ml bottling is saved backup, then by spare sperm in foam plastic reservoir slow cooling 15 minutes, dropped to temperature
When to 17-18 DEG C, it is put into 17.2 DEG C of constant temperature refrigerator and saves, sperm bottle is gently overturn 3-5 times every 12 hours, makes sperm
It is sufficiently mixed with dilution, prevents Sperm pellets and cause death；It can be reserved for 10 days in 17.2 DEG C of constant temperature；
5. artificial insemination：The spare sperm of 70ml is inputted with sow of the vas deferens to heat, after 8 hours, by the spare sperm of 70ml
It is placed in 25 DEG C of warm water after heating 5-10min, is incorporated 2ml oxytocin, shakes up, second of semen deposition is carried out to sow, can be improved
Sow number of eggs ovulated and litter size.
As a kind of preferred fertilization method for improving farrowing efficiency, the heat sow is the heat mother to wean 5-6 days
Pig.
As a kind of preferred fertilization method for improving farrowing efficiency, when artificial insemination of the present invention, heat sow exists
Its genital tract is cleaned before first time semen deposition.
The present invention can be summarized with others without prejudice to the concrete form of spirit or essential characteristics of the invention.Therefore, nothing
By from the point of view of which point, the embodiment above of the invention can only all be considered the description of the invention and cannot limit invention,
Claims indicate the scope of the present invention, and above-mentioned explanation does not point out the scope of the present invention, therefore, with the present invention
The comparable meaning and scope of claims in any variation, be all considered as being included within the scope of the claims.
Claims (3)
1. a kind of fertilization method for improving farrowing efficiency, which is characterized in that include the following steps：
1. the preparation before semen collection：Distilled water 500ml, dilution powder 25g are placed in 1000ml beaker, stirring and dissolving is heated up to 37
DEG C, constant temperature is spare；Upper disposable special plastic bag is covered on the collection essence cup of 500ml, and covers 3 layers of filter paper, then uses cushion rubber
Filtering paper edge is fixed on rim of a cup, makes to be located inside beaker in the middle part of filter paper；
2. semen collection：Semen collection is carried out to three of the right age boars respectively, semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterile glove,
After boar, which climbs up phantom, stretches out penis, penis front end spire is held with the right hand, and with thumb top glans penis, then pass through
Rhythmically pinching and penis being pulled back and forth, boar being made to generate pleasant sensation and ejaculate, thumb and index finger are slightly opened into dew when ejaculation
Glans penis out allows sperm to project, and the thin sperm without sperm projected at first is not collected, and is rich in the milky white of sperm wait project
It is just combined in cup with semen collection cup when color sperm, when there is fibrin clot, exhausts the first time sperm that boar shoots away with thumb, so
The right hand still rhythmically pinching and pulls penis afterwards, with stimulate boar penis continue second and third time ejaculate, boar is penetrated
Essence terminate to back off in advance dummy when, the right hand gently takes advantage of a situation and send penis to foreskin, and semen collection terminates, three of the right age boar semens point
It Wei not sperm I, sperm II and sperm III；
3. sperm motility rate and density inspection judgement：The sperm adopted is checked to the motility rate and density of sperm under the microscope, is judged
Whether sperm can be used, and determine the extension rate of sperm, and concrete operations are as follows：
The judgement of a motility rate：Collected sperm is dripped on glass slide, is observed under 400 power microscopes, before straight line in the visual field
The percentage of relative number and sperm sum into the sperm of movement determines the vigor of sperm, is generally evaluated with reagent,
100% sperm straight ahead movement is chosen as level-one motility rate, and 90% sperm straight ahead movement is chosen as 0.9 grade of motility rate, with such
It pushes away, 10% sperm straight ahead movement is chosen as 0.1 grade of motility rate, and the sperm of linear forward travel is known as effective sperm of being fertilized；
The judgement of b sperm concentration：Ocular estimate judges rating by the density of sperm intuitive in the visual field, is divided under the microscope
It is close, in, it is dilute third；
It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, contains essence in every milliliter of sperm
Son is about 300,000,000 or more；
In：There are many sperm seen in the visual field, but have apparent gap between sperm and sperm, between sperm away from
Length from about 1 sperm, while single sperm motility is it is clear that contain sperm about 1-3 hundred million in every milliliter of sperm；
It is dilute：A small number of sperms are seen in the visual field, gap is very big to each other for sperm, and gap is more than the length of two sperms, and every milliliter
In sperm containing sperm count about below 100,000,000；
4. determining effective sperm, mixing, dilution, bottling：Sperm survival rate be 0.9 grade or 1 grade, and density be it is close or in sperm
For effective sperm, by effective sperm I, then sperm II and sperm III mixing press 1 with dilution:The mixing of 3-4 dilution proportion is equal
After even, every bottle of 70ml bottling is saved backup, then by spare sperm in foam plastic reservoir slow cooling 15 minutes, dropped to temperature
When to 17-18 DEG C, it is put into 17.2 DEG C of constant temperature refrigerator and saves, sperm bottle is gently overturn 3-5 times every 12 hours, makes sperm
It is sufficiently mixed with dilution, prevents Sperm pellets and cause death；It can be reserved for 10 days in 17.2 DEG C of constant temperature；
5. artificial insemination：The spare sperm of 70ml is inputted with sow of the vas deferens to heat, after 8 hours, by the spare sperm of 70ml
It is placed in 25 DEG C of warm water after heating 5-10min, is incorporated 2ml oxytocin, shakes up, second of semen deposition is carried out to sow, can be improved
Sow number of eggs ovulated and litter size.
2. a kind of fertilization method for improving farrowing efficiency according to claim 1, it is characterised in that：The heat sow is
The heat sow of wean 5-6 days.
3. a kind of fertilization method for improving farrowing efficiency according to claim 1, it is characterised in that：The artificial insemination
When, heat sow cleans its genital tract before first time semen deposition.
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| CN201810687706.8A CN108836564A (en) | 2018-06-28 | 2018-06-28 | A kind of fertilization method improving farrowing efficiency |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105982765A (en) * | 2015-02-11 | 2016-10-05 | 垫江县优科畜牧有限公司 | Artificial insemination method for pigs |
| CN106510892A (en) * | 2016-12-26 | 2017-03-22 | 王邦才 | Artificial fertilization method for sows |
| CN106963512A (en) * | 2017-04-09 | 2017-07-21 | 剑河县康科畜牧发展有限公司 | The Artificial insemination procedures of one boar |
| CN107802373A (en) * | 2017-10-30 | 2018-03-16 | 石阡裕和原生态农牧发展有限公司 | A kind of boar semen collection platform and its semen collection method |
-
2018
- 2018-06-28 CN CN201810687706.8A patent/CN108836564A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105982765A (en) * | 2015-02-11 | 2016-10-05 | 垫江县优科畜牧有限公司 | Artificial insemination method for pigs |
| CN106510892A (en) * | 2016-12-26 | 2017-03-22 | 王邦才 | Artificial fertilization method for sows |
| CN106963512A (en) * | 2017-04-09 | 2017-07-21 | 剑河县康科畜牧发展有限公司 | The Artificial insemination procedures of one boar |
| CN107802373A (en) * | 2017-10-30 | 2018-03-16 | 石阡裕和原生态农牧发展有限公司 | A kind of boar semen collection platform and its semen collection method |
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Application publication date: 20181120 |
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