CN106963512A - The Artificial insemination procedures of one boar - Google Patents

Abstract

The present invention relates to the Artificial insemination procedures of swine rearing method, more particularly to a boar, belong to cultural technique field.Comprise the following steps：1）Preparation before semen collection；2）Free-hand semen collection；3）Sperm motility rate and density inspection judge；4）Artificial insemination.Semen collection of a breeding boar of the invention can breed and can breed 300~1000 in 3~8,1 year, and breeding head number is 3~7 times of natural mating, greatly reduce cultivation kind pig's head number, aquaculture cost is reduced, and this method conception rate is high, product subnumber is more, and economic benefit is more notable.

Description

The Artificial insemination procedures of one boar

Technical field

The present invention relates to the Artificial insemination procedures of swine rearing method, more particularly to a boar, belong to cultural technique field.

Background technology

At present, most pig farms are to use natural breeding, i.e., when oestrus of sow is motionless, catch up with boar and sow mating by Pregnant, boar is caught up with excessive, sperm survival rate can be caused low, and dead essence rate is high, causes conception rate and product subnumber to decline；In addition, natural Mating is easily caused bacterium, the propagation of virus, adds the probability of sow infectious disease；Furthermore, natural mating mode, one is good Plant boar and be once only capable of the sow that mates, sperm utilization rate is relatively low, a boar at most 100~150 mothers of mating in 1 year Pig, the need for meeting breeding, the cultivation amount of boar is big, and cost is higher, and economic benefit is relatively low.At present, also there is part cultivation Field is bred using Artificial insemination procedures, but because technology is not mature enough, causes pregnancy rate and farrowing rate relatively low, economic benefit It is not notable enough.

The content of the invention

In order to overcome the deficiencies in the prior art, it is an object of the invention to provide the artificial insemination side of a boar Method, the semen collection of breeding boar one time of this method one can breed and can breed 300~1000 in 3~8,1 year, and breeding head number is 3~7 times of natural mating, greatly reduce cultivation kind pig's head number, reduce aquaculture cost, and this method conception rate is high, production Subnumber is more, and economic benefit is more notable.

To achieve these goals, the present invention uses following technical scheme：The Artificial insemination procedures of one boar, including it is following Step：

1）Preparation before semen collection：Distilled water 500ml, dilution powder 25g are placed in 1000ml beakers, stirring and dissolving waits until dilution Liquid, is heated up to 37 DEG C and constant temperature is standby；The disposable special plastic bag on set on the 500ml smart cup of collection, and cover 3 layers of filtering Paper, is then fixed on rim of a cup with cushion rubber by filter paper edge, makes to be located inside beaker in the middle part of filter paper；

2）Free-hand semen collection：Semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterilization Rubber gloves, squats in dummy rear, work as boar Climb up after dummy emersion penis, penis front end spire is held with the right hand, and with thumb top glans penis, then by there is rhythm Ground pinching and penis is pulled back and forth, boar is produced pleasant sensation and is ejaculated, thumb and forefinger are somewhat opened during ejaculation and expose penis Head, allows seminal fluid to project, and the thin seminal fluid without sperm projected at first is refused to collect, and waits to project the milky seminal fluid rich in sperm Shi Caiyong semen collections cup is combined in cup, when there is fibrin clot, the first time seminal fluid that boar of being drained with thumb shoots away, then the right hand Still rhythmically pinching and penis is pulled, continued with stimulating boar penis second and third time is ejaculated, boar ejaculation knot Beam back off in advance dummy when, the right hand is gently taken advantage of a situation and delivers to penis in foreskin, and semen collection terminates；

3）Sperm motility rate and density inspection judge：The seminal fluid adopted is checked to the motility rate and density of sperm under the microscope, judged Whether seminal fluid can be used, and determine the extension rate of seminal fluid, and concrete operations are as follows：

A motility rates judge：The seminal fluid collected is dripped in being placed on observation under 400 power microscopes on slide, by straight in the visual field The relative number of the sperm of line forward travel judges the vigor of sperm with the percentage of sperm sum, is typically commented with reagent Fixed, 100% sperm straight ahead motion is chosen as one-level motility rate, and 90% sperm straight ahead motion is chosen as 0.9 grade of motility rate, successively Analogize, 10% sperm straight ahead motion is 0.1 grade of motility rate, and the sperm of linear forward travel is referred to as effective sperm of being fertilized；For Simple and convenient, usable test paper is measured；

B sperm concentrations judge：Ocular estimate, under the microscope, rating is carried out by the density of sperm directly perceived in the visual field, be divided into it is close, In, it is dilute third.It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, every milliliter of seminal fluid In containing sperm about more than 300,000,000.In：The sperm seen in the visual field is a lot, but has obvious space between sperm and sperm, The length of about 1 sperm of distance between sperm, while single sperm motility is it is clear that contain sperm in every milliliter of seminal fluid About 1~300,000,000.It is dilute：A small number of sperms are only seen in the visual field, the space of sperm to each other is very big, length of the gap more than two sperms In degree, every milliliter of seminal fluid containing sperm count about below 100,000,000；In order to simple and convenient, sperm concentration tester can be used to be measured；

C determines effective seminal fluid, dilution, bottling：Sperm survival rate be 0.9 or 1 grade, and density be it is close or in seminal fluid be effective essence Liquid, 1 is pressed by effective seminal fluid and dilution：After 3~4 dilution proportions are well mixed, every bottle of 70ml bottling is saved backup, then will be standby With seminal fluid in foam plastic reservoir slow cooling 15 minutes, when temperature is down to 17~18 DEG C, be put into 17.2 DEG C of constant temperature refrigerator It is middle to preserve, seminal fluid bottle is gently overturn 3~5 times every 12 hours, seminal fluid is sufficiently mixed with dilution, prevent Sperm pellets and Cause death；It can be preserved in 17.2 DEG C of constant temperature 10 days；

4）Artificial insemination：The standby seminal fluid of 70ml is inputted to the sow of heat with vas deferens, every after 8 hours, by the standby seminal fluid of 70ml It is placed in 25 DEG C of warm water and heats after 5~10min, be incorporated 2ml oxytocins, shake up, second of semen deposition is carried out to sow, can be improved Sow number of eggs ovulated and litter size.

In above-mentioned technical proposal, step 1）Described in dilution powder for Guangzhou Bai Xu symphysis agricultural corporation Belgian seminal fluid it is dilute Release powder.

Compared with prior art, the present invention due to use above technical scheme, have the advantages that for：1）It will steam Distilled water 500ml is mixed with dilution powder 25g, and the dilution liquid energy made maintains the optimal pH value of sperm survival, can be to greatest extent The breeding of microorganism in seminal fluid, the energy required for the effective accessory substance for neutralizing sperm metabolism generation and offer sperm are provided Material.The diluted seminal fluid prepared by this proportioning, seminal fluid can be preserved 10 days under 17.2 DEG C of states, greatly extend essence The holding time of liquid.2）Dilution is heated up to 37 DEG C and constant temperature is standby, 37 DEG C are closest with the seminal fluid temperature that just collects, The active degree of sperm can be made to reach optimum state, the convenient survival rate and density for accurately judging sperm, from rational judgement And effective seminal fluid is utilized, the utilization rate of seminal fluid and the success rate of fertilization are improved, temperature is too low or too high, easily causes the dead of sperm Die.3）Filtered using 3 layers of filter paper, can effectively improve the purity of seminal fluid.4）The free-hand semen collection method of the present invention, can make kind Pig all projects seminal fluid, effectively raises the utilization rate of kind of pig semen, reduces the cultivation head number of boar, reduces raiser Aquaculture cost, so as to improve the economic benefit of raiser.5）By accurately judging the density and survival rate of sperm, effectively Raising fertilization success rate and litter size.6）Effective seminal fluid and dilution are pressed 1：3~4 dilution proportions are mixed, each semen deposition 70ml, in the case where not wasting seminal fluid, can guarantee that the quantity of effective sperm reaches maximum, thus effectively improve ripe smart rate and Litter size.7）Seminal fluid bottle is gently overturn 3~5 times every 12 hours, seminal fluid is sufficiently mixed with dilution, prevents Sperm pellets And cause death.8）After first time semen deposition, be spaced 8 hours, by the standby seminal fluid of 70ml be placed in 25 DEG C of warm water heating 5~ After 10min, 2ml oxytocins are incorporated, are shaken up, second of semen deposition is carried out to sow, sow number of eggs ovulated and litter size can be improved.9）Adopt Manually be fertilized, can overcome boar body greatly with sow body is small mate when inconvenience contradiction, be also prevented from the mutual infection of disease. 10）Using the method for the invention, the semen collection of a breeding boar can breed and can breed 300~1000 in 3~8,1 year Head, breeding head number is 3~7 times of natural mating, greatly reduces cultivation kind pig's head number, so as to reduce aquaculture cost and add The fast development and the improvement of boar of swinery；The method of the invention conception rate is high, product subnumber is more, improves the economy of raiser Benefit.

Embodiment

With reference to specific embodiment, the invention will be further described：

Embodiment 1, the Artificial insemination procedures of a boar, comprise the following steps：

1）Preparation before semen collection：The Belgian semen dilution powder that distilled water 500ml, Guangzhou Bai Xu symphysis agricultural corporation are sold 25g is placed in 1000ml beakers, and stirring and dissolving waits until dilution, is heated up to 37 DEG C and constant temperature is standby；On the 500ml smart cup of collection Disposable special plastic bag is put, and covers 3 layers of filter paper, filter paper edge is then fixed on rim of a cup with cushion rubber, makes filtering It is located in the middle part of paper inside beaker；

2）Free-hand semen collection：Semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterilization Rubber gloves, squats in dummy rear, work as boar Climb up after dummy emersion penis, penis front end spire is held with the right hand, and with thumb top glans penis, then by there is rhythm Ground pinching and penis is pulled back and forth, boar is produced pleasant sensation and is ejaculated, thumb and forefinger are somewhat opened during ejaculation and expose penis Head, allows seminal fluid to project, and the thin seminal fluid without sperm projected at first is refused to collect, and waits to project the milky seminal fluid rich in sperm Shi Caiyong semen collections cup is combined in cup, when there is fibrin clot, the first time seminal fluid that boar of being drained with thumb shoots away, then the right hand Still rhythmically pinching and penis is pulled, continued with stimulating boar penis second and third time is ejaculated, boar ejaculation knot Beam back off in advance dummy when, the right hand is gently taken advantage of a situation and delivers to penis in foreskin, and semen collection terminates；

3）Sperm motility rate and density inspection judge：The seminal fluid adopted is checked to the motility rate and density of sperm under the microscope, judged Whether seminal fluid can be used, and determine the extension rate of seminal fluid, and concrete operations are as follows：

A motility rates judge：The seminal fluid collected is dripped in being placed on observation under 400 power microscopes on slide, by straight in the visual field The relative number of the sperm of line forward travel judges the vigor of sperm with the percentage of sperm sum, is typically commented with reagent Fixed, 100% sperm straight ahead motion is chosen as one-level motility rate, and 90% sperm straight ahead motion is chosen as 0.9 grade of motility rate, successively Analogize, 10% sperm straight ahead motion is 0.1 grade of motility rate, and the sperm of linear forward travel is referred to as effective sperm of being fertilized；For Simple and convenient, usable test paper is measured；

B sperm concentrations judge：Ocular estimate, under the microscope, rating is carried out by the density of sperm directly perceived in the visual field, be divided into it is close, In, it is dilute third.It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, every milliliter of seminal fluid In containing sperm about more than 300,000,000.In：The sperm seen in the visual field is a lot, but has obvious space between sperm and sperm, The length of about 1 sperm of distance between sperm, while single sperm motility is it is clear that contain sperm in every milliliter of seminal fluid About 1~300,000,000.It is dilute：A small number of sperms are only seen in the visual field, the space of sperm to each other is very big, length of the gap more than two sperms In degree, every milliliter of seminal fluid containing sperm count about below 100,000,000；In order to simple and convenient, sperm concentration tester can be used to be measured；

C determines effective seminal fluid, dilution, bottling：Sperm survival rate be 0.9 or 1 grade, and density be it is close or in seminal fluid be effective essence Liquid, 1 is pressed by effective seminal fluid and dilution：After 3~4 dilution proportions are well mixed, every bottle of 70ml bottling is saved backup, then will be standby With seminal fluid in foam plastic reservoir slow cooling 15 minutes, when temperature is down to 17~18 DEG C, be put into 17.2 DEG C of constant temperature refrigerator It is middle to preserve, seminal fluid bottle is gently overturn 3~5 times every 12 hours, seminal fluid is sufficiently mixed with dilution, prevent Sperm pellets and Cause death；It can be preserved in 17.2 DEG C of constant temperature 10 days；

4）Artificial insemination：The standby seminal fluid of 70ml is inputted to the sow of heat with vas deferens, every after 8 hours, by the standby seminal fluid of 70ml It is placed in 25 DEG C of warm water and heats after 5~10min, be incorporated 2ml oxytocins, shake up, second of semen deposition is carried out to sow, can be improved Sow number of eggs ovulated and litter size.

Specific description is done with reference to control case 1~3 and 4~6 pairs of effects of the present invention of test case：

Compare case 1：2004, the present inventor was responsible for work of breeding on the domestic pig farm in Jie Yang County Guangdong Provice one, and the pig farm is equal It is to take natural mating mode to be bred, design parameter is as shown in Table 1：

Table one

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					8	89	75	84.2	10.2
9	126	108	85.7	10.1
					10	104	86	82.6	9.9

Compare case 2：2009, the present inventor was responsible for work of breeding on the domestic pig farm in Guizhou Province Xiuwen County one, and the pig farm is equal It is to take natural mating mode to be bred, sow is more, and boar is few, and boar overuses, design parameter is as shown in Table 2：

Table two

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					4	78	66	84.6	9.92
5	113	94	83.1	9.91
					8	126	102	81	9

Compare case 3：2010, the present inventor was responsible for work of breeding on the domestic pig farm of Guizhou Province's Fuquan one, and the pig farm is Natural mating mode is taken to be bred, design parameter is as shown in Table 3：

Table three

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					4	72	62	86.1	10.1
10	81	72	88.9	9.3
					11	74	65	87.8	9.9

Test case 4：2011, the present inventor was responsible for the artificial insemination work of pig on the domestic pig farm in zunyi, guizhou city one, specifically Parameter is as shown in Table 4：

Table four

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					3	93	85	91.3	12.2
4	81	73	90.1	12.3
					5	103	94	91.2	12.2

Test case 5：2013, the present inventor was responsible for the artificial insemination work of pig, specific ginseng on the domestic pig farm of Guizhou Jianhe one Number is as shown in Table 5：

Table five

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					5	9	9	100	12.6
6	8	8	100	11.9
					7	12	11	92	12.3

Test case 6：2015, the present inventor was responsible for the artificial insemination work of pig, specific ginseng on the domestic pig farm in Guizhou Kweiyang one Number is as shown in Table 6：

Table six

Month	Mate sow number	Pregnant number	Pregnancy rate（%）	Average litter size（Head）
					6	50	48	96	14
7	68	66	97	12.1
					8	63	60	95	12.6

It was found from above-mentioned control case 1~3 and test case 4~6, the pregnancy rate of natural breeding is 82.6~88.8%, average production Young number is 9.3~10.2, and artificial insemination breeding pregnancy rate is 90.1~100%, and average litter size is 11.9~14, therefore, The method of the invention can effectively improve pregnancy rate and litter size, so as to improve the economic benefit of raiser.

Claims (2)

1. the Artificial insemination procedures of a boar, it is characterised in that comprise the following steps：

1）Preparation before semen collection：Distilled water 500ml, dilution powder 25g are placed in 1000ml beakers, stirring and dissolving waits until dilution Liquid, is heated up to 37 DEG C and constant temperature is standby；The disposable special plastic bag on set on the 500ml smart cup of collection, and cover 3 layers of filtering Paper, is then fixed on rim of a cup with cushion rubber by filter paper edge, makes to be located inside beaker in the middle part of filter paper；

2）Free-hand semen collection：Semen collection person's left hand pinches the smart cup of collection, and the right hand puts on sterilization Rubber gloves, squats in dummy rear, work as boar Climb up after dummy emersion penis, penis front end spire is held with the right hand, and with thumb top glans penis, then by there is rhythm Ground pinching and penis is pulled back and forth, boar is produced pleasant sensation and is ejaculated, thumb and forefinger are somewhat opened during ejaculation and expose penis Head, allows seminal fluid to project, and the thin seminal fluid without sperm projected at first is refused to collect, and waits to project the milky seminal fluid rich in sperm Shi Caiyong semen collections cup is combined in cup, when there is fibrin clot, the first time seminal fluid that boar of being drained with thumb shoots away, then the right hand Still rhythmically pinching and penis is pulled, continued with stimulating boar penis second and third time is ejaculated, boar ejaculation knot Beam back off in advance dummy when, the right hand is gently taken advantage of a situation and delivers to penis in foreskin, and semen collection terminates；

3）Sperm motility rate and density inspection judge：The seminal fluid adopted is checked to the motility rate and density of sperm under the microscope, judged Whether seminal fluid can be used, and determine the extension rate of seminal fluid, and concrete operations are as follows：

A motility rates judge：The seminal fluid collected is dripped in being placed on observation under 400 power microscopes on slide, by straight in the visual field The relative number of the sperm of line forward travel judges the vigor of sperm with the percentage of sperm sum, is typically commented with reagent Fixed, 100% sperm straight ahead motion is chosen as one-level motility rate, and 90% sperm straight ahead motion is chosen as 0.9 grade of motility rate, successively Analogize, 10% sperm straight ahead motion is 0.1 grade of motility rate, and the sperm of linear forward travel is referred to as effective sperm of being fertilized；

B sperm concentrations judge：Ocular estimate, under the microscope, rating is carried out by the density of sperm directly perceived in the visual field, be divided into it is close, In, it is dilute third；

It is close：Sperm is full of whole visual field, interspaces between sperm small, less than the length of a sperm, containing smart in every milliliter of seminal fluid Son is about more than 300,000,000；

In：The sperm seen in the visual field is a lot, but have between sperm and sperm between obvious space, sperm away from From the length of about 1 sperm, while single sperm motility is it is clear that contain sperm about 1~300,000,000 in every milliliter of seminal fluid；

It is dilute：A small number of sperms are only seen in the visual field, the space of sperm to each other is very big, gap more than two sperms length, often In milliliter seminal fluid containing sperm count about below 100,000,000；

C determines effective seminal fluid, dilution, bottling：Sperm survival rate be 0.9 or 1 grade, and density be it is close or in seminal fluid be effective essence Liquid, 1 is pressed by effective seminal fluid and dilution：After 3~4 dilution proportions are well mixed, every bottle of 70ml bottling is saved backup, then will be standby With seminal fluid in foam plastic reservoir slow cooling 15 minutes, when temperature is down to 17~18 DEG C, be put into 17.2 DEG C of constant temperature refrigerator It is middle to preserve, seminal fluid bottle is gently overturn 3~5 times every 12 hours, seminal fluid is sufficiently mixed with dilution, prevent Sperm pellets and Cause death；It can be preserved in 17.2 DEG C of constant temperature 10 days；

4）Artificial insemination：The standby seminal fluid of 70ml is inputted to the sow of heat with vas deferens, every after 8 hours, by the standby seminal fluid of 70ml It is placed in 25 DEG C of warm water and heats after 5~10min, be incorporated 2ml oxytocins, shake up, second of semen deposition is carried out to sow, can be improved Sow number of eggs ovulated and litter size.

2. the Artificial insemination procedures of pig as claimed in claim 1, it is characterised in that step 1）Described in dilution powder be Guangzhou it is white The Belgian semen dilution powder of rising sun symphysis agricultural corporation.