CN108834979A - A kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method - Google Patents
A kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method Download PDFInfo
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- CN108834979A CN108834979A CN201810978348.6A CN201810978348A CN108834979A CN 108834979 A CN108834979 A CN 108834979A CN 201810978348 A CN201810978348 A CN 201810978348A CN 108834979 A CN108834979 A CN 108834979A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation methods, include the following steps：Step 1: shrimp seed rearing：2 × 2 × 1.5 meters of concrete sowing ponds are built, shrimp seed of macrobrachium rosenbergii is selected, cultivates at room temperature two weeks and adapt it to environment；Step 2:, with lime disinfection, selecting the hydrilla verticillata of stem length 20cm or more in 6 × 3 × 2.2 × 1.8 natural mud sump pond, interval is planted in pond bottom, continues culture one week；It is fed Step 3: the shrimp seedling after adapting in step 1 is moved in pond, launches organic granular feed, persistently feed 20 weeks or more.The present invention utilizes the growing environment of hydrilla verticillata and Macrobrachium rosenbergii symbiosis, and one side hydrilla verticillata can provide food source abundant for Macrobrachium rosenbergii, while can improve water quality, be provided for good habitat.Therefore, demand when feeding the abundance of food less than natural cultivation, while improving the amino acid composition and content of Macrobrachium rosenbergii muscle, its trophic level can be improved, improve meat.
Description
Technical field
The present invention relates to aquaculture field more particularly to a kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation methods.
Background technique
In recent years, the development of aquaculture is increasingly used in improving the quality of aquatic products.With people's living standard
Raising, consumer increasingly pays close attention to the muscle quality of aquatic products, nutritional quality and safety.Equally, peasant be more concerned about how
The aquatic products with high muscle quality and flavor are obtained, to obtain higher economic benefit.It has been reported that the rouge of culture species
Fat content and muscle quality are different in different culture environments, this further results in the improvement of muscle yield and quality.Also
Document report, better water correction can be with the quality and flavor of fish and shrimp muscle.
The nutrient content of different culture environment of aquatic products and cultivation species and the concentration and type of existing harmful substance
Difference it is related, which reflects the differences of nutrient present in its food and toxin.Growth performance, muscular components and muscle matter
The difference on ground eventually leads to the variation of muscle quality.Therefore, the selection of culture environment plays important in the quality of breeding kind
Effect.Culture model appropriate is developed to keep the speed of growth, while also improving the muscular components of culture species, flavor substance and
Quality is the important directions of aquaculture research.
In the past, raising fish and shrimp faced problems in natural pond, if muscle quality is poor, mouthfeel or flavor are bad, more held
Easy infection disease etc. causes product quality decline in turn result in peasant economy loss.Many studies have shown that being cultivated with natural pond
Prawn compare, ecologic breeding prawn shows higher growth, immunity, muscle quality and flavor
Macrobrachium rosenbergii is because of its cheap price, ideal taste and flavor, nutrition, fast-growth, to the neurological susceptibility of disease
And with the abilities of other aquatile symbiosis and at home with it is very popular in export market, the species the Indian Ocean-too
The subtropical and tropical zones for putting down ocean cultivate extensively, and particularly in China, India is Malaysian, Thailand and Vietnam, usually in
It is cultivated in small-sized soil pond, although the pond culture of Macrobrachium rosenbergii causes global yield to be significantly increased, meat is not obtained very
Therefore good raising studies Macrobrachium rosenbergii symbiosis ecologic breeding, improves its Lean mass, flavor components and muscle quality, be to learn
The common demand of person and market.
Summary of the invention
In order to solve shortcoming present in above-mentioned technology, the present invention provides a kind of Macrobrachium rosenbergii shrimp grass symbiosis ecologies
Cultural method.
In order to solve the above technical problems, the technical solution adopted by the present invention is that：A kind of Macrobrachium rosenbergii shrimp grass symbiosis ecology
Cultural method includes the following steps：
Step 1: shrimp seed rearing：2 × 2 × 1.5 meters of concrete sowing ponds are built, select shrimp seed of macrobrachium rosenbergii, at room temperature
It cultivates two weeks and adapts it to environment；
Step 2:, with lime disinfection, selecting stem length 20cm or more in 6 × 3 × 2.2 × 1.8 natural mud sump pond
Hydrilla verticillata, interval are planted in pond bottom, continue culture one week；
It is fed Step 3: the shrimp seedling after adapting in step 1 is moved in pond, launches organic granular feed, it is lasting to feed
20 weeks or more.
Further, in the step 1, breeding method is：Three times, feeding bait is artemia living without section children to daily feeding
The mixture of body, boiled chicken protein and organic granular feed, day feeding volume account for the 6%~8% of shrimp body total amount；It replaces weekly
30% water, the polybag of oxygenation is added in pond, and holding water oxygen content is 5.2 ± 0.3mg/L.
Further, in the step 1, the weight ratio of each feeding bait is 3: 3: 4 in one day.
Further, in the step 3, feeding method is：Three times, feeding bait is crude protein content to daily feeding
36%~38% pellet, day feeding volume account for the 6%~7% of shrimp body total amount, and water oxygen content is kept to be greater than 5.0mg/
L。
Further, in the step 3, the weight ratio of each feeding bait is 2: 3: 5 in one day.
The contents of the present invention further include application of the above-mentioned ecological cultivation method in terms of improving Macrobrachium rosenbergii meat and nutrition.
The present invention utilizes the growing environment of hydrilla verticillata and Macrobrachium rosenbergii symbiosis, and one side hydrilla verticillata can be Roche natural pond
Shrimp provides food source abundant, while can improve water quality, is provided for good habitat.Therefore, the abundance of food is fed
Demand when less than natural cultivation, while improving the amino acid composition and content of Macrobrachium rosenbergii muscle, its trophic level can be improved,
Improve meat.The present invention simultaneously by being cultivated to Macrobrachium rosenbergii-hydrilla verticillata shrimp grass symbiosis when bait content and feeding grind
Study carefully, select most suitable feeding volume, while save the cost, meat more can be improved, obtain good technical effect.
Specific embodiment
The present invention will be further described in detail with reference to the specific embodiments.
【Embodiment 1】
The present embodiment is related to a kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method, including：
Step 1: shrimp seed rearing：2 × 2 × 1.5 meters of concrete sowing ponds are built, select shrimp seed of macrobrachium rosenbergii, at room temperature
It cultivates two weeks and adapts it to environment；Breeding method is：Three times, feeding bait is artemia nauplii, boiled egg living to daily feeding
The mixture of albumin and organic granular feed, day feeding volume account for the 6%~8% of shrimp body total amount；Each feeding bait in one day
Weight ratio be 3: 3: 4；The polybag of oxygenation is added in pond for the water of replacement 30% weekly, keep water oxygen content be 5.2 ±
0.3mg/L,
Step 2:, with lime disinfection, selecting stem length 20cm or more in 6 × 3 × 2.2 × 1.8 natural mud sump pond
Hydrilla verticillata 5kg, interval are planted in pond bottom, continue culture one week；
It is fed Step 3: the shrimp seedling after adapting in step 1 is moved in pond, launches organic granular feed, it is lasting to feed
20 weeks or more；Feeding method is：Three times, feeding bait is the pellet of crude protein content 36%~38%, day to daily feeding
Feeding volume accounts for the 6%~7% of shrimp body total amount, and water oxygen content is kept to be greater than 5.0mg/L, the weight of each feeding bait in one day
Amount is than being 2: 3: 5.
【Embodiment 2】
The research of the present embodiment design Macrobrachium rosenbergii shrimp grass Symbiotic Model.
1. material prepares
Macrobrachium rosenbergii is collected from Chinese Nanning country Macrobrachium rosenbergii hatchery, and is transferred to the concrete of two standards
It sows in pond (2 × 2 × 1.5 meters), the polybag of oxygenation is added, and adapt to two weeks at room temperature before the experiments.In the laundering period
Between, three times a day (08：00,12：00 and 17：00) artemia nauplii living to shrimp seedling feeding, boiled chicken protein and commercially available
Organic granular particle (Guangzhou fishery), in one day the weight ratio of each feeding bait be 3: 3: 4.30% water is updated weekly,
And water oxygen content (DO) is maintained by 5.2 ± 0.3mg/L. hydrilla verticillata by aeration and develops limited public affairs purchased from Jiangsu aquatic products
Department.
After adaptation, (1.02 ± 0.11 centimetres of average length of 1,200 tail Macrobrachium rosenbergii；0.18 ± 0.01 gram of average weight) point
For 3 experimental groups and 1 control group (CG), every group is repeated 3 times (i.e. every group 100) duplication).By the shrimp seedling of 3 experimental groups with
Plant H.verticillata (hydrilla verticillata) symbiosis；CG postlarva is cultivated in the repetition natural pond of not plant.?
During experiment, water oxygen content (D0) maintains 5.0mg/L or more.Since to consume wheel blade black for the prawn in cogeneration system
Algae, their number of animals raised are lower than the standard number of the pellet of business preparation；That is 30% (D30) of standard bait, 50%
(D50) and 70% (D70).All groups are placed in the standard mud sump of 6 × 3 × 2.2 × 1.8 depth.During experiment in 20 weeks,
Shrimp in natural pond random feeding three times (08 daily：00,12：00 and 17：00), in one day each feeding bait weight ratio
It is 2: 3: 5.
The excessive bait of daily siphon before first time feeding, drying simultaneously save.Dead shrimp is taken out daily and is recorded.
It is 20 weeks during experiment, research low spot carries out in state key aquaculture base (Guangxi China Nanning).In this experiment, often
Surrounding measures growth parameter(s), and determines other parameters at the end of experiment.
2. sampling
Isosorbide-5-Nitrae on the day of sampling, the sample for randomly choosing 100 tail shrimps for 8,12,16 and 20 weeks from each repetition are used for
Measure growth parameter(s).At the end of feeding experiment, all Macrobrachium rosenbergiis are taken out, and are randomly choosed in 24 hours each duplicate
100 tail shrimps.20 tails are selected to measure the water content (WHC) of muscle, 20 tail body muscle samples (2 × 2 × 2cm) and at -60 DEG C
Lower storage, then to measure crude protein, thick lipid, ash content, moisture, amino acid and malonaldehyde (MDA).20 tail samples separately are taken,
Excision intersects fin muscle samples (2 × 2cm), fixes 3 days with 10% buffered formalin, is dehydrated, and paraffin embedding is thin for muscle
Born of the same parents' analysis；MDA of the 20 tail samples for measurement is horizontal and 20 tail shrimps are for measuring sapidity ingredient.
3. growth parameters
At the end of feeding experiment, measure shrimp weight and length and calculate to calculate growth parameter(s)：Weight gain (WG),
Length increases (LG), feeds than (FR), specific growth rate (SGR), efficiency of food conversion.(FCR) and protein efficiency ratio (PER).
WG (g)=final weight (g)-original body mass (g)
LG (cm)=long (cm)-first initial body of final body is long (cm)
FR (g d-1)=100 × [dry feed intake (g)/day]
SGR (%d-1)=100 × [(final weight (the g)-ln original body mass (g) of ln)/day]
FCR=dry feed intake (g)/weight in wet base increases (g)
PER=weight in wet base increases (g)/protein intake (g)
4. muscle composition analysis
Thick protein, thick lipid, moisture and crude ash content are measured according to AOAC (2003) method.Use Kjeldahl's method
Measure crude protein content.Thick lipid content is measured using Soxtherm (SOX 416Macro, Gerhardt, Germany).Pass through
Burning sample 6 hours determines content of ashes at 550 DEG C in Muffle furnace.According to AOAC (2003) and Funatsu etc., analysis
The amino acid of peptide (including oligopeptides) forms.Muscle samples are analyzed using High-Speed Automatic amino-acid analyzer：By sample 2.5mL mistake
Formic acid hydrolyzes 20 minutes at 55 DEG C, and injects in the unit with sodium exchange column and carry out methionine (Met) analysis.For MDA
Analysis refrigerates body flesh sample (5g) at -18 DEG C, and fine gtinding simultaneously thaws 12 hours.Using kit, (Nanjing bioengineering is ground
Study carefully institute, Nanning, China) measurement MDA level.
5. flavor forms
Nucleic acid related compound is measured in efficient lipid chromatograph using built-in column, is walked using the detection of following methods
Suddenly：Buffer (0.05mol/L KH is loaded with the flow velocity of 0.8mL/min2P04, 0.05mol/L K2HP04, pH 6.85), it uses
Ultraviolet specrophotometer measures nucleic acid related compound in 254nm.
6. muscle quality
Slice is cut from each paraffin mass and is dyed with hematoxylin and eosin, the shooting of use research grade intelligence micrometron
The region epaxial (muscle region above vertebra).The region epaxial of four parts is randomly choosed, to use image procossing
Quantify the fiber area and density of white muscle with analysis software.Fibre density is expressed as fiber count/muscle area.
Measure the water content WHC of muscle：Two pieces of muscle masses are steamed to 5 minutes at 100 DEG C in the gauze to determine that steam damages
It loses.Other two block boils 5 minutes in 100 DEG C of boiling water to determine cooking loss.Then, sample is cooled down at room temperature, is used
Blotting paper wipes surface water, then weighs.Vapour losses and cooking loss are measured using Equivalent Calculation, as from initial sample weight
Measure the weight percent of loss.By two blocks with 5000 × g centrifugation 15 minutes to carry out centrifugation loss measurement, and at -20 DEG C
Storage 24 hours.Two blocks thaw at room temperature then to carry out freezing loss measurement.Calculate centrifugation loss and freezing loss simultaneously
It is expressed as the percentage of original specimen weight loss.
7. statistical analysis
Statistical analysis is carried out using SPSS 16.0.For two groups, mean expression ± standard deviation (SD).By unidirectional
ANOVA and compare treatment group using the subsequent Multiple range test that the test of the multiregion of Duncan carries out.Significant property level set is P
< 0.05.
8. result counts
Growth parameter(s) such as table 1 in 8.1 Macrobrachium rosenbergiis 20 weeks
Growth parameter(s) in 1 Macrobrachium rosenbergii of table 20 weeks
During culture one week, without significant difference in terms of LG, WG or PER between CG and three experimental group, but for 4
To 20 weeks culture periods, LG, WG and the PER of D70 is significant to be higher than value.In CG, D30 and D50.In FR between CG and three experimental group
And significant difference is detected in terms of SGR and between all groups during cultivating for 1 to 20 week.The SGR of D70 is significant to be higher than CG,
D30 and D50, but the FR of D70, D30 and D50 are significant lower than CG (P < 0.05).During cultivating for 1 to 4 week, CG and three test
It without significant difference in terms of FCR between group, but was 8 to 20 weeks for culture period, the FCR of D70 is significant to be higher than CG, D30 and D50
Value.Relative to CG, LG, WG, SGR, FCR and PER value rise 21.80%-50.01%, 31.32%- respectively in D70
72.34%, 21.84%-50.10%, 18.97%-32.22% and 16.92%-41.17%, the FR of D70 is lower than CG's
14.78%-27.63%.
Compare growth parameter(s), muscle composition, flesh between the prawn cultivated in prawn plant symbiosis system and natural pond
Meat flavor ingredient and muscle quality.In the prawn botanical system containing 70% protein bait, compared with the control group, Roche natural pond
The significant raising of the growth performance of shrimp.The feeding rate (FR) of the D70 prawn of culture 1-20 weeks is lower than CG prawn, but other growth indexes
It is above CG.Water plant is containing there are many nutrition and minerals, it may be possible to provide food source full of nutrition, while can also change
Kind water quality, it can be seen that hydrilla verticillata can promote the growth of Macrobrachium rosenbergii, while reduce bait dosage.D30 and D50 prawn
Growth parameter(s) be lower than CG and D70 prawn, although this shows that prawn plant symbiosis system can provide good habitat for it.
Prawn, it is still desirable to which a certain amount of protein maintains to grow.Therefore, when protein feed level is up to 70%, Roche natural pond
Shrimp shrimp grass cogeneration system seems beneficial to cultivating.
The muscle chemical composition such as table 2 of 8.2 Macrobrachium rosenbergiis 20 weeks, 20 weeks free amino acids of Macrobrachium rosenbergii composition such as table 3,
The amino acid composition such as table 4 of Macrobrachium rosenbergii the 20th week peptide (including oligopeptides).
2 Macrobrachium rosenbergii of table, 20 weeks muscle chemical composition (mg 100g-1, weight in wet base)
3 20 weeks free amino acids of Macrobrachium rosenbergii of table form (mg 100g-1, weight in wet base)
The amino acid of peptide (including oligopeptides) in 4 Macrobrachium rosenbergii of table the 20th week forms (mg 100g-1, weight in wet base)
Table 2 shows that the muscle moisture and coarse ash between each group do not have significant difference (P > 0.05), and the thick egg in D70
White level is significant to be higher than CG, D30 and D50 (P < 0.05)；The thick lipid level of D70 is significant to be lower than CG, D30 and D50 (P <
0.05).MDA in D30, D50 and D70 is horizontal significant lower than CG, and the value in D70 is minimum.Table 3 show, D30, D50 and
Glycine (Gly) and glutamic acid (Glu) content in the muscle samples of D70 are higher than CG (P < 0.05), the value highest in D70.
Total free amino acid concentration in D70 muscle samples is higher than CG, D30 and D50 (P < 0.05).Certain specific amino acids in shrimp
Content is higher, and D70 muscle samples Glutamic Acid (Gly), arginine (Arg), alanine (Ala) and proline (Pro) content are most
Height, but CG value is minimum (P < 0.05).Gly (32.92%), Arg (24.03%), Ala (9.43%) and Pro (7.59%) are accounted for
The 73.97% of total free amino acid in D70 muscle samples.Gly (29.92%), Arg (13.26%), Ala (17.67%) and
Pro (6.97%) constitutes 67.82% of total free amino acid in CG muscle samples.The ammonia of peptide of the display of table 4 including oligopeptides
Base acid composition.The total amino acid total content of muscle samples from CG, D30 and D50 is higher than D70, but the value highest in CG.Glu,
Gly, Arg, Pro and Ala are main components.This 5 kinds of amino acid there are concentration be higher than muscle samples in other amino acid, account for
The 72.16% of D70 muscle samples total amount, accounts for the 71.54% of CG total amount.
Biochemical composition is also the good index of shellfish physiological status, is easy to be commented can cultivate in biology
Estimate.Studies have shown that habitat condition will affect the biochemical composition of Macrobrachium rosenbergii：In the biochemical composition of Macrobrachium rosenbergii muscle, water
Point, protein, amino acid and content of fatty acid change with the difference of culture environment.In our current research, Macrobrachium rosenbergii exists
D70, D30 and D50 are significant higher relative to protein content in the muscle samples of CG, the significant reduction of lipid content.Show wheel blade
Black grass promotes the absorption and accumulation of protein in Macrobrachium rosenbergii.MDA participates in lipid-metabolism；High MDA content table in general fish
Bright high peroxidatic reaction of lipid, reflects tissue damage.In our current research, the MDA of the prawn muscle from D70, D30 and D50
Content is higher than CG.The observation result can reflect the lower lipid content of prawn in prawn plant symbiosis system.
Meanwhile shellfish muscle contains the free amino acid of high concentration, especially Gly, Pro, Arg, Glu and Ala.
These amino acid contents are higher than control pond in the muscle samples of D70, D50 and D30 prawn in shrimp grass symbiosis cultivation.This shows
The amino acid composition and content of Macrobrachium rosenbergii muscle depend on their habitat condition.
Flavor components composition such as table 5 in the 20th week muscle of 8.3 Macrobrachium rosenbergiis
Flavor components in muscle in 5 Macrobrachium rosenbergii of table the 20th week form (mg 100g-1, weight in wet base)
ADP, adenosine diphosphate (ADP)；AMP, adenylic acid；ATP, atriphos；CMP, cytidine monophosphate；GMP, one phosphorus of guanosine
Acid；Hx, hypoxanthine；HxR, inosine；IMP, inosine monophosphate.
The AMP of D70 muscle sample, guanosine monophosphate (GMP) and IMP content are higher than CG, D30 and D50 (P < 0.05), and
Atriphos (ATP), cytidine monophosphate (CMP), HxR and hypoxanthine (Hx) content are lower (P < 0.05).CG,D30,D50,D70
In muscle samples trimethylamine oxide content be respectively 4.33 ± 0.68,4.29 ± 0.72,4.48 ± 0.59,4.62 ±
0.71mg/100g, difference is without significant property (P > 0.05).The beet alkali content of D70 sample is significant to be higher than CG, D30 and D50 (P <
0.05)。
In fish, in prawn and shellfish muscle, there are a series of physiology function more than 100 nucleotide and related compound
Energy.The reason of some ingredients are delicate flavour senses, in particular, IMP and GMP stimulates delicate flavour and enhances the delicate flavour of glutamic acid.In this research
In, it is horizontal to be higher than CG for the content of delicate flavour nucleotide IMP and GMP in D70 prawn muscle.
8.4.8.3 the 20th week muscle quality of Macrobrachium rosenbergii composition such as table 6
Table 6 shows that the fiber area of D30, D50 and D70 are significant to be higher than CG, the fiber area highest (P < 0.05) of D70.
The fibre density of D30, D50 and D70 are significant to be lower than CG, and the value of D70 is minimum (P < 0.05).The fiber area ratio CG high of D70
48.78% (P < 0.05), but low 17.30% (the P < 0.05) of fibre density of D70.The boiling and boiling of D70 muscle samples are damaged
Lose points 19.03% and 10.84% (P < 0.05) not lower than CG, D30 and D50.Centrifugation loss or freezing loss are without significant between each group
Difference (P > 0.05).
Cooking loss and vapour losses reflect the comprehensive loss of liquid and solable matter in muscle, are mainly due to water
Loss.The vapour losses of Macrobrachium rosenbergii muscle in shrimp grass symbiotic cultivation system and cooking loss are lower than to be cultivated in natural pond
Prawn.These observation indicate that, high muscle water content in D70, D30 and D50 and provide the prawn plant of more preferable water quality
Cogeneration system is related.In addition, the consumption to hydrilla verticillata can promote the collagen synthesis in muscle, so as to improve the matter of muscle
Ground.
Muscle cell is the index of muscle quality.In our current research, relative to CG shrimp, Roche in D70, D30 and D50 shrimp
The meat fiber area of pond crayfish is significant higher, the significant reduction of density.Show that Macrobrachium rosenbergii generates in prawn plant symbiosis system
The muscle of high quality.
Above embodiment is not limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The variations, modifications, additions or substitutions that the technical staff in domain is made within the scope of technical solution of the present invention, also belong to this hair
Bright protection scope.
Claims (6)
1. a kind of Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method, which is characterized in that include the following steps：
Step 1: shrimp seed rearing：2 × 2 × 1.5 meters of concrete sowing ponds are built, shrimp seed of macrobrachium rosenbergii is selected, cultivates at room temperature
Adapt it to environment within two weeks；
Step 2:, with lime disinfection, selecting the wheel blade of stem length 20cm or more in 6 × 3 × 2.2 × 1.8 natural mud sump pond
Black algae, interval are planted in pond bottom, continue culture one week；
It is fed Step 3: the shrimp seedling after adapting in step 1 is moved in pond, launches organic granular feed, persistently fed 20 weeks
More than.
2. Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method according to claim 1, it is characterised in that：The step 1
In, breeding method is：Three times, feeding bait is that artemia nauplii, boiled chicken protein and organic granular living are raised to daily feeding
The mixture of material, day feeding volume account for the 6%~8% of shrimp body total amount；30% water is replaced weekly, and the plastics of oxygenation are added in pond
Bag, holding water oxygen content are 5.2 ± 0.3mg/L.
3. Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method according to claim 2, it is characterised in that：The step 1
In, the weight ratio of each feeding bait is 3: 3: 4 in one day.
4. Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method according to claim 3, it is characterised in that：The step 3
In, feeding method is：Three times, feeding bait is the pellet of crude protein content 36%~38%, day feeding volume to daily feeding
The 6%~7% of shrimp body total amount is accounted for, water oxygen content is kept to be greater than 5.0mg/L.
5. Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method according to claim 4, it is characterised in that：The step 3
In, the weight ratio of each feeding bait is 2: 3: 5 in one day.
6. Macrobrachium rosenbergii shrimp grass symbiosis ecological cultivation method described in any bar is improving Macrobrachium rosenbergii meat in claim 1-5
With the application in terms of nutrition.
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