CN108813139A - A kind of prawn physiological health enhancing feed addictive - Google Patents
A kind of prawn physiological health enhancing feed addictive Download PDFInfo
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- CN108813139A CN108813139A CN201810867155.3A CN201810867155A CN108813139A CN 108813139 A CN108813139 A CN 108813139A CN 201810867155 A CN201810867155 A CN 201810867155A CN 108813139 A CN108813139 A CN 108813139A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
- A23K20/126—Lactones
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A kind of prawn physiological health enhancing feed addictive, its components include vitamin C 0.03%, vitamin E 0.02%, bile acid 0.05%, andrographolide 0.1%, chlorogenic acids 0.1%, and preparation process is simple, and cost is relatively low.The reinforcing agent prawn culturing using it is rear have effects that improve prawn fat metabolism, antioxidant levels, the ability of immune defense and have promote prawn grow.The composite fortifier that the prawn physiological health reinforcing agent is made of Chinese herbal medicine, vitamin, bile acid, with the ability for improving prawn physiological health integral level, reduce the use of drug, meet the requirement of prawn culturing production, the present invention is not only that prawn healthy aquaculture provides technical support, also provides important leverage for prawn green cultivation.
Description
Technical field
The present invention relates to a kind of prawn physiological healths to enhance feed addictive, belongs to aquatic product matched feed additive technology neck
Domain.
Background technique
China's culture fishery flourishes in recent years, and shrimp culture industry occupies highly important in world aquaculture
Status, China is as the first big country of prawn culturing yield, it is necessary to ensure the healthy and stable development of prawn culturing industry, at present prawn
Aquaculture is to pursue the production model of high-yield and high-efficiency, and excessive protein and fat are added in feed, causes rouge in prawn body
Fat accumulation, metabolic disorder；It is excessive to use chemicals in order to control the generation of disease, cause the physiological health of prawn horizontal
Increasingly decline, disease is spread unchecked.Current main direction of studying is one-sided using some nutrients, microelement, Chinese herbal medicine etc.
Physiological function in a certain respect is improved, such as nutrition, immune, metabolism etc., has a single function, is unable to satisfy the normal physiology of prawn
Health is badly in need of a kind of reinforcing agent of comprehensive physiological health level, immune to improve prawn, ensures that prawn nutritional need promotes prawn
Healthy growth.
Summary of the invention a
In view of the above-mentioned problems, the purpose of the present invention is to provide a kind of prawn physiological healths to enhance feed addictive, by raising
Vitamin C, vitamin E, andrographolide, five kinds of bile acid, chlorogenic acid substances are added in material, enhance prawn immune defense energy
Power promotes prawn healthy growth, keeps the physiological health of prawn.
In order to achieve the above objectives, the specific technical solution that the present invention takes is：
A kind of prawn physiological health enhancing feed addictive, it is characterised in that based on mass fraction, it includes vitamin C 5%-
15%, vitamin E 5%-15%, bile acid 10%-30%, andrographolide 30%-50%, chlorogenic acid 0-40%.
The prawn physiological health enhances feed addictive, it is characterised in that vitamin C：Vitamin E：Bile acid：It wears
The mass ratio of heart lotus lactone is 3：2：5：10.
The prawn physiological health enhances feed addictive, it is characterised in that vitamin C：Vitamin E：Bile acid：Gallbladder
Juice acid：Andrographolide：The mass ratio of chlorogenic acid is 3：2：5：10：10.
The vitamin C, vitamin E are purchased from Hangzhou Ai Limai animal pharmaceutical estate Co., Ltd, and the bile acid is purchased from
Yu Longchang Dong Bao Co., Ltd.
The prawn physiological health enhances feed addictive, which is characterized in that the chlorogenic acid, andrographolide are logical
Cross following methods preparation：
The chlorogenic acid is after uniformly crushing sheet Cortex Eucommiae, with the ethyl acetate containing 0. 05 mol/L hydrochloric acid according to quality
Volume ratio is 1:10 mix, and leaching 2 hours is stirred at reflux in 65 DEG C of water-baths, repeat to extract 2 times, merging filtrate, rotation is steamed
Hair is to get chlorogenic acid；
The andrographolide is the effective component of Herba Andrographitis, extracting method be after Herba Andrographitis is uniformly crushed with 95% second
Alcohol is according to 1:10 mass volume ratio mixes, and decocts 2h, is repeated 1 times, merging filtrate, rotary evaporation is to get andrographolide.
The prawn physiological health enhancing feed addictive is preparing the application in prawn culturing feed.
A kind of prawn feed containing prawn physiological health enhancing feed addictive, it is characterised in that the feed is on basis
It is obtained after addition vitamin C, vitamin E, bile acid, andrographolide in feed；And dimension is added in every kilogram of basal feed
Raw element C0.3g, vitamin E 0.2g, bile acid 0.5g, andrographolide 1g.
A kind of prawn feed containing prawn physiological health enhancing feed addictive, it is characterised in that the feed is on basis
It is obtained after addition vitamin C, vitamin E, bile acid, andrographolide, chlorogenic acid in feed；And in every kilogram of basal feed
Add vitamin C 0.3g, vitamin E 0.2g, bile acid 0.5g, andrographolide 1g, chlorogenic acid 1g.
The prawn feed, it is characterised in that the basal feed based on mass fraction, fish meal 34%, dregs of beans 20%,
Peanut meal 16.4%, wheat flour 22%, soybean lecithin 5%, multi-vitamins 0.6%, composite mineral matter 2%.
The advantages of the present invention：
（1）Improve litopenaeus vannamei haemocyte quantity.In prawn nonspecific immunity system, haemocyte plays important angle
Color is both the supplier of the executor of cellular immunity and humoral immunity.Feeding prawn physiological health enhancing feed addictive
14 days in, test group prawn haemocyte quantity is all remarkably higher than control group（P<0.05）, and control group is without significant difference.
（2）Improve litopenaeus vannamei haemocyte phagocytic activity.Haemocyte can be wrapped up by phagocytosis, destroyed and be eliminated
Foreign substance, therefore, haemocyte phagocytic activity can be used for measuring the ability that litopenaeus vannamei resists exotic invasive pathogen.?
In 21 days for feeding prawn physiological health enhancing feed addictive, test group prawn haemocyte phagocytic activity is all remarkably higher than control
Group（P<0.05）, and control group is without significant difference.
（3）Improve litopenaeus vannamei antibacterial activity.Antibacterial is the important indicator when Body fluid immunity, in external germ
When infecting body, antibacterial plays important protective effect.It is all in 21 days for feeding prawn physiological health enhancing feed addictive
Shore prawn Serum bactericidal vigor of receiving is significantly improved, significant difference between each test group and control group（P<0.05）, and control group
Without significant difference.
（4）Improve litopenaeus vannamei total antioxidant capacity.Total antioxidant capacity index embody body include enzyme and
The overall antioxidant activity of all antioxidant of non-enzyme.Enhance 21 days of feed addictive feeding prawn physiological health
Interior, test group prawn haemocyte phagocytic activity is all remarkably higher than control group（P<0.05）, and control group is without significant difference.
（5）Improve litopenaeus vannamei lipase activity.Lipase is the crucial enzyme of prawn body fat metabolism, influences body
The synthesis and metabolism of interior fat, play an important role in digestive system.Feeding prawn physiological health enhancing feed addition
In 21 days of agent, Serum of Penaeus vannamei lipase activity is significantly improved, significant difference between each test group and control group（P<
0.05）.
（6）Litopenaeus vannamei is improved to the resistivity of morbid vibrio.7d after injection Vibrio harveyi, control group prawn
Cumulative mortality be 69.15%, the cumulative mortality of 1 prawn of test group is 32.35%, the cumulative mortality of 2 prawn of test group
It is 20.15%, significant difference between test group and control group（P<0.05）.
Test demonstrates a kind of prawn physiological health enhancing feed addictive provided by the present invention and can be obviously promoted pair
Shrimp growth, enhances the immunity of prawn, improves the ability that prawn resists the disease；The metabolism for promoting fat mitigates the negative of hepatopancrease
Load, to promote prawn healthy growth.The reinforcing agent selects vitamin C, vitamin E, bile acid, andrographolide, chlorogenic acid
Five kinds of substances carry out scientific compatibility, are added in feed to qualitative, quantitative, are conducive to the stability and standard metaplasia that maintain drug effect
It produces, present invention preparation is simple, and cost is relatively low, can be improved the economic benefit of shrimp culture industry.
Detailed description of the invention
Fig. 1 is the result figure of prawn haemocyte quantity in embodiment 2.
Fig. 2 is the result figure of prawn haemocyte phagocytic activity in embodiment 2.
Fig. 3 is the result figure of prawn blood plasma antibacterial activity in embodiment 2.
Fig. 4 is the result figure of prawn hepatopancrease total antioxidant capacity in embodiment 2
Fig. 5 is the result figure of prawn lipase activity in embodiment 2.
Fig. 6 is the result figure of prawn cumulative mortality in embodiment 2.
Specific embodiment
Embodiment 1：
A kind of prawn physiological health enhances the preparation of feed addictive and breeding feed
After sheet Cortex Eucommiae is uniformly crushed, with ethyl acetate（Containing 0.05 mol/L hydrochloric acid）It is 1 according to mass volume ratio:8 mix,
It is stirred at reflux leaching 3 hours in 65 DEG C of water-baths, repeats to extract 2 times, merging filtrate isolates ethyl acetate, obtains chlorogenic acid.
It after Herba Andrographitis is uniformly crushed, sets in round-bottomed flask, adds 95% ethyl alcohol, flow back 1 hour, flow back 2 times altogether, merging filtrate is stood
Overnight, it filters and obtains andrographolide.
According to above-mentioned preparation method prepare respectively two kinds of various concentrations prawn physiological health enhancing feed addictive and
Corresponding breeding feed.Based on mass fraction, a kind of prawn physiological health enhancing feed addictive includes every kilogram of feed addition
Vitamin C 0.3g, vitamin E 0.2g, bile acid 0.5g, andrographolide 1g；Another prawn physiological health enhancing feed adds
Adding agent includes every kilogram of feed addition vitamin C 0.3g, vitamin E 0.2g, bile acid 0.5g, andrographolide 1g, chlorogenic acid
1g。
The content of said herbal medicine effective component can be measured according to method provided by existing literature.In Herba Andrographitis
The measuring method reference of ester content：HPLC method measures the content of andrographolide in Herba Andrographitis and its extract, in contemporary Chinese
Medicine, the 08th phase of volume 3 in 2001；The measuring method reference of chlorogenic acid content：Journal of Pharmaceutical
Analysis, the 04th phase of volume 1 in 2011.
Embodiment 2：
A kind of prawn physiological health enhancing feed addictive to litopenaeus vannamei non-specific immunity and lipase activity and
The influence of oxidation resistance
In this test, two kinds of prawn physiological healths that inventor prepares embodiment 1 enhance feed addictive, to its non-specificity
Immune indexes and lipase activity are measured, it is intended to be filtered out and be improved litopenaeus vannamei non-specific immune function and anti-oxidant
Ability and the physiological health reinforcing agent for promoting prawn fat metabolism.
1 materials and methods
1.1 material
Litopenaeus vannamei is used in test（Litopenaeus vannamei）It is purchased from Laoshan District sand mouth prawn culturing factory of Qingdao City, is put down
Equal weight is 7.35 ± 0.31 grams, and the ingredient of test basal feed is：Based on mass fraction, fish meal 34%, dregs of beans 20%, flower
The raw dregs of rice 16.4%, wheat flour 22%, soybean lecithin 5%, multivitamin 0.6%, composite mineral matter 2%.Through being sought in experiment calculation feed
It forms and is divided into：Based on mass fraction, crude protein 42.4%, crude fat 7.2%, ash content 8.4%.
1.2 method
1.2.1 experimental design
After test is temporarily supported 7 days with litopenaeus vannamei, test is divided into 3 groups with prawn, every group sets 3 repetitions, and each repeating groups are put
Support 70 tail shrimps.Test group is divided into：Control group, test group 1, test group 2, control group feed basal feed, test 1 and 2 group of difference
Feed the basal feed of two kinds of enhancing feed addictives of physiological health containing prawn prepared by embodiment 1.Feed physiology containing prawn
After health enhancing feed addictive 28 days, every group takes 30 tail shrimps to carry out challenge test at random, with 10 tail shrimps for a repetition, each
3 repetitions of test group.Vibrio harveyi is pressed 1 × 106Cell/mL concentration is in the end intramuscular injection of prawn periproct（50 μ L/ tails）.
During test, feed daily 2 times, day feeding volume is the 5% of weight, respectively at 9:00,16：00 respectively feeds half.
1.2.2 testing index and method
It samples within the 0th, 7,14,21,28 day in on-test.Prawn is inserted directly into using No. 5 syringe needles and 1mL syringe of disinfection
Blood was collected by 3mm or so in carapace rear cardiocoelom, has sucked that the improved pre-cooling of 0.3mL is all to be received before drawing blood in syringe in advance
Shore prawn anti-coagulants（0.34M NaCl, 0.01M KCl, 0.01M EDTA-Na2, 0.01M HEPES, PH 7.45, infiltration
Pressure is 780 mOsm.kg thoroughly-1）, finally making the ratio of anti-coagulants and hemolymph is 1:1.A part of hemolymph sample carries out blood
The measurement of cell quantity and phagocytic rate, remaining hemolymph sample after 800g centrifugation 10min, take blue supernatant, as blood at 4 DEG C
Slurry samples save under the conditions of -80 DEG C, while taking prawn hepatic tissue, with liquid nitrogen grinding, save under the conditions of being put into -80 DEG C,
Lipase activity to be measured.
The measurement of haemocyte quantity is counted under an optical microscope using blood counting chamber；The measurement side of phagocytic activity
Method is operated according to the method for existing literature, is referred to：Immune responses and expression of immune-
related genes in swimming crab Portunus trituberculatus exposed to elevated
ambient ammonia-N stress. Comparative Biochemistry and Physiology Part A；Antibacterial
Vigor, Bacteriolytic activity measuring method according to existing literature method operate, be referred to：Insect immunity.
Purification and properties of three inducible bactericidal proteins from
hemolymph of immunized pupae of Hyalophora cecropia. European Journal of
Biochemistry；Total antioxidant capacity builds up biological Co., Ltd using Nanjing and produces kit measurement.The measurement of lipase
Method can refer to：Studies on the digestive lipases of milkfish.Aquaculture；Protease
Measuring method can refer to：The estimation of pepsin, trypsin, papain, and cathepsin
with hemoglobin..The Journal of general physiology。
2 results
2.1 survivals and growth
| Processing group | Rate of body weight gain（%） | Survival rate（%） | Specific growth rate（%） |
| Control group | 82.9.13 ±3.2a | 94.32±3.2a | 2.12±0.23a |
| Test group 1 | 90.4±4.3b | 95.18±2.1a | 2.41±0.62b |
| Test group 2 | 109.25±5.6b | 95.16±2.6a | 2.72±0.71b |
As seen from table：Feed 28 days of a kind of prawn physiological health enhancing feed addictive are fed, wherein litopenaeus vannamei increases weight
Rate and specific growth rate significantly improve, test group significant difference compared with the control group（P<0.05）, survival rate aspect 2 He of test group
1 phase of test group is above control group, and test group 1 and 2 difference of test group is not significant（P > 0.05）.Contain the above result shows that feeding
A kind of Feed Energy of prawn physiological health enhancing feed addictive improves survival rate, rate of body weight gain and the specific growth rate of prawn.
2.2 haemocyte quantity
From Fig. 1：Behind feed 21 days for feeding the enhancing feed addictive containing a kind of prawn physiological health, litopenaeus vannamei blood
Cell quantity is significantly increased, and after the test, compared with the control group, the haemocyte quantity of test group 1 and 2 increases separately 16.2%
With 18.5%, test group and control group significant difference（P<0.05）.But test group 2 is compared with test group 1, and addition chlorogenic acid fails
Further increase the quantity of haemocyte.
2.3 haemocyte phagocytic activity
As shown in Figure 2：Behind feed 7 days for feeding the enhancing feed addictive containing a kind of prawn physiological health, litopenaeus vannamei blood is thin
Born of the same parents' phagocytic rate starts to be significantly higher than control group, in 14 days to 21 days test groups 1 and 2 significant difference of test group（P<0.05）Show with
The extension of culturing time, chlorogenic acid be conducive to further promoted litopenaeus vannamei haemocyte phagocytic activity.After 21 days
The prawn haemocyte phagocytic activity of test group 1 and test group 2 is declined, and test group is significantly higher than control group always（P<
0.05）.
2.4 antibacterial activity
As shown in Figure 3：Behind feed 7 days for feeding the enhancing feed addictive containing a kind of prawn physiological health, litopenaeus vannamei antibacterial
Activity starts to increase, and reaches peak in the 21st day test group 1 and 2 Serum bactericidal activity of test group, with control group difference
Significantly（P<0.05）.From the 21st day to off-test, two test group blood antibacterial activities are declined slightly, but still are significantly higher than control
Group.Entire test period, test group 1 and 2 antibacterial activity difference of test group is not significant.It can obtain, add in feed based on the above results
Add vitamin C, vitamin E, bile acid, andrographolide that can improve Serum of Penaeus vannamei antibacterial activity, but adds chlorogenic acid
It cannot continue to enhance prawn Serum bactericidal activity.
2.5 total antioxidant capacity
As seen from Figure 4：Behind feed 7 days for feeding the enhancing feed addictive containing a kind of prawn physiological health, litopenaeus vannamei always resists
Oxidability starts to be significantly higher than control group, reaches peak, test group total antioxidation in 14 days to 21 days total antioxidant capacity
Ability is all remarkably higher than control group（P<0.05）, 2 significant difference of test group 1 and test group（P<0.05）, illustrate that test group 2 is added
Chlorogenic acid is conducive to improve prawn total antioxidant capacity.
2.6 lipase activity
From Fig. 5：Behind feed 7 days for feeding the enhancing feed addictive containing a kind of prawn physiological health, litopenaeus vannamei fat
Enzyme is significantly increased, and after the test, compared with the control group, the haemocyte quantity of test group 1 and 2 increases separately 9.2% and 10.5%,
Test group and control group significant difference（P<0.05）.But test group 2 is compared with test group 1, and addition chlorogenic acid fails further to increase
Lipase vigor.
2.7 cumulative mortality
As shown in Figure 6：Attack poison after 0.5 day each group prawn start dead, every group of death 1-2 tail occur, when by 1 day, control group pair
Shrimp adds up death toll and is apparently higher than each test group, and when by 3 days, each group death toll is reduced, and after 5 days, each group prawn death toll reduces
And it tends towards stability.At the end of challenge test, control group cumulative mortality is up to 69.15%, and 1 cumulative mortality of test group is
32.35%, 2 cumulative mortality 30.15% of test group, show to feed addition vitamin C, vitamin E, andrographolide, chlorogenic acid,
The Feed Energy of bile acid improves the anti-vibrios ability of litopenaeus vannamei, and the anti-vibrios ability of test group 2 is more preferable.
Claims (8)
1. a kind of prawn physiological health enhances feed addictive, which is characterized in that based on mass fraction, it includes vitamin C 5%-
15%, vitamin E 5%-15%, bile acid 10%-30%, andrographolide 30%-50%, chlorogenic acid 0-50%.
2. prawn physiological health as described in claim 1 enhances feed addictive, which is characterized in that vitamin C：Vitamin E：
Bile acid：The mass ratio of andrographolide is 3：2：5：10.
3. prawn physiological health as described in claim 1 enhances feed addictive, which is characterized in that vitamin C：Vitamin E：
Bile acid：Andrographolide：The mass ratio of chlorogenic acid is 3：2：5：10：10.
4. prawn physiological health as described in any one of claims 1-3 enhances feed addictive, which is characterized in that described is green
Ortho acid, andrographolide are prepared by the following method：
The chlorogenic acid is after uniformly crushing sheet Cortex Eucommiae, with the ethyl acetate containing 0. 05 mol/L hydrochloric acid according to quality
Volume ratio is 1:10 mix, and leaching 2 hours is stirred at reflux in 65 DEG C of water-baths, repeat to extract 2 times, merging filtrate, rotation is steamed
Hair is to get chlorogenic acid；
The andrographolide is the effective component of Herba Andrographitis, extracting method be after Herba Andrographitis is uniformly crushed with 95% second
Alcohol is according to 1:10 mass volume ratio mixes, and decocts 2h, is repeated 1 times, merging filtrate, rotary evaporation is to get andrographolide.
5. a kind of described in any item prawn physiological health enhancing feed addictives of claim 1-3 are preparing prawn culturing feed
In application.
6. a kind of prawn physiological health enhances feed addictive, it is characterised in that the feed is to add vitamin in basal feed
C, it is obtained after vitamin E, bile acid, andrographolide；And vitamin C 0.3g, vitamin are added in every kilogram of basal feed
E0.2g, bile acid 0.5g, andrographolide 1g.
7. a kind of prawn feed containing prawn physiological health enhancing feed addictive, it is characterised in that the feed is raised on basis
It is obtained after addition vitamin C, vitamin E, bile acid, andrographolide, chlorogenic acid in material；And add in every kilogram of basal feed
Add vitamin C 0.3g, vitamin E 0.2g, bile acid 0.5g, andrographolide 1g, chlorogenic acid 1g.
8. prawn feed as claimed in claims 6 or 7, it is characterised in that the basal feed based on mass fraction, fish meal
34%, dregs of beans 20%, peanut meal 16.4%, wheat flour 22%, soybean lecithin 5%, multi-vitamins 0.6%, composite mineral matter 2%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998161A (en) * | 2021-04-08 | 2021-06-22 | 中国热带农业科学院热带生物技术研究所 | Chinese herbal medicine hepatopancreatic protective agent and preparation method and application thereof |
| CN115553403A (en) * | 2022-11-03 | 2023-01-03 | 上海海洋大学 | Litopenaeus vannamei meat quality improver and preparation method and application thereof |
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| CN104664174A (en) * | 2015-03-20 | 2015-06-03 | 珠海海龙生物科技有限公司 | Anti-stress health compound feed for jewfish |
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| CN106942521A (en) * | 2017-02-28 | 2017-07-14 | 中国海洋大学 | What a kind of property of medicine was stablized is used for prawn Immune-enhancing effect and sterilized Chinese herbal and crude drugs preparations and method |
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| CN103265509A (en) * | 2013-05-28 | 2013-08-28 | 江西中医学院 | Method for extracting active ingredients of andrographis paniculata in vacuum degree regulation and control auxiliary mode |
| CN104664174A (en) * | 2015-03-20 | 2015-06-03 | 珠海海龙生物科技有限公司 | Anti-stress health compound feed for jewfish |
| CN106551178A (en) * | 2016-10-25 | 2017-04-05 | 南宁海世界生物科技有限公司 | Prawn feed and preparation method thereof |
| CN106942521A (en) * | 2017-02-28 | 2017-07-14 | 中国海洋大学 | What a kind of property of medicine was stablized is used for prawn Immune-enhancing effect and sterilized Chinese herbal and crude drugs preparations and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112998161A (en) * | 2021-04-08 | 2021-06-22 | 中国热带农业科学院热带生物技术研究所 | Chinese herbal medicine hepatopancreatic protective agent and preparation method and application thereof |
| CN115553403A (en) * | 2022-11-03 | 2023-01-03 | 上海海洋大学 | Litopenaeus vannamei meat quality improver and preparation method and application thereof |
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Application publication date: 20181116 |